pycom01g17210

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002740
Physical Location & Seq
Forward (+)
16582253 .. 16586859
4607 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3264 bp
ATGTTCAGTCATTATGACCGTCCTCGTATTGCCCAACTATGCGAAAAGGCTGGTCTTTATGTGCGAGCTTTGCAATACTCAGAGTTGCCTGATATTAAACGTGTCATAGTGAACACACATGCAATAGAGCCACAGTCACTTGTAGAGTTTTTTGGCACTCTATCAAAAGAATGGGCATTGGAGTGCATGAAAGATCTTTTATTGGTCAACTTAAGAGGAAACCTTCAGATAATAGTGCAGGTTGCTAAGGAATACTCTGAGCAGTTGGGAGTTGATCAGTGCATGAAGCTCTTTGAACAGTTCAAGTCATATGAAGGGTTGTATTTCTTTCTTGGTTCGTTTTTGAGCTCAAGTGAGGATCCTGATATCCACTTCAAGTACATTGAGGCAGCTGCTAAAACTGGACAAATCAAAGAGGTGGAGCGAGTTACTAGAGAATCGAACTTCTATGATGCTGAGAAGACGAAGAACTTCTTAATGGAAGCGAAGCTCCCCGATGCACGTCCATTGATTAATGTTTGTGATCGTTTTGGATTTGTTCCAGATCTCACCCACTACTTGTACACGAACAATATGCTTCGTTACATTGAAGGTTACGTTCAGAAGGTGAACCCTGGGAATGCTCCATTAGTTGTGGGACAGTTGCTAGATGATGAGTGCCCTGAAGATTTTATCAAAGGTTTAATTCTTTCTGTCCGTTCATTGCTTCCAGTGGAGCCCCTTGTGGAGGAATGTGAGAAAAGGAATCGCCTTCGTTTACTCACACAGTTCTTGGAGCATCTTGTGAGTGAGGGAAGCCAAGATGTACATGTTCACAATGCTTTGGGTAAAATTATCATTGATAGCGGCAATAATCCAGAACACTTCCTTACCACAAACCCATATTATGATTCTCGTGTTGTTGGTAAATATTGTGAGAAACGTGATCCTACCCTGGCTGTAGTTGCTTACCGCCGTGGACAATGTGACGATGAACTCATTAATGTCACAAATAAGAACTCCTTGTTCAAATTGCAAGCAAGGTATGTGGTTGAGAGGATGGATGAAGATCTTTGGGGAAAAGTTCTTGACCCTGAGAATGAGTACAGAAGACAGCTTATTGATCAAGTTGTTTCTACTGCCTTGCCCGAAAGCAAGAGCCCGGAACAAGTTTCTGCGGCTGTTAAGGCTTTCATGACTGCTGATTTGCCCCATGAGTTAATTGAGCTTCTTGAAAAGATTGTGCTTCAAAACTCTGCCTTCAGTGGGAACTTTAATCTGCAAAACCTGCTAATCTTGACCGCCATCAAGGCAGATCCATCAAGAGTTATGGATTATATTAATAGGCTTGATAATTTTGATGGACCTGCAGTTGGGGAAGTAGCTGTGGAAGCCCAACTGTTTGAGGAAGCGTTTGCAATCTTCAAGAAGTTTAACTTGAATGTCCAAGCTGTTAATGTCTTGCTGGATAACATCCGAAGCATTGATAGGGCTGTTGAGTTTGCGTTCCGTGTGGAAGAAGATGCTGTTTGGAGCCAGGTTGCCAAGGCTCAGCTCCGGGAGGGTCTGGTGAGCGATGCTATTGAGTCATTTATCCGTGCAGATGATGCCACCCAATTTTTGGATGTTATTCGCGCTTCTGAGGATGCAGATGTGTACCATGACTTGGTGAGGTATCTTCTGATGGTTAGGCAAAAGGTGAGAGAGCCCAAGGTGGACAGTGAGCTCATTTATGCATATGCAAAGATTGATAGGCTTGCTGACATTGAAGAATTTATTCTTATGCCCAATGTCGCTAATCTCCAAAATGTTGGTGATCGCCTATATGACGAGGCTCTCTATGAGGCTGCAAAGATAATATATGCCTTCATATCTAACTGGGCGAAGTTGGCTGTCACACTTGTGAAGCTCAAACAATTTCAAGGTGCTGTTGATGCAGCACGAAAAGCCAACAGTTCAAAGACATGGAAGGAGGTTTGCTTTGCTTGTGTTGATGCTGAGGAGTTCCGTTTAGCTCAGATTTGTGGTCTCAACATTATTATACAGGTGGATGATTTGGAAGAGGTCAGTGAATACTACCAGAATAGAGGATGCTTCAACGAGCTAATATCTCTAATGGAGAGTGGTTTGGGGTTAGAACGTGCTCACATGGGAATCTTTACTGAGTTGGGGGTTCTATATGCTAGATATCGCCCAGAGAAACTCATGGAGCACATCAAATTGTTCGCAAATAGGCTAAATATTCCTAAGCTTATAAGAGCATGTGATGAGCAGCAACATTGGAAGGAGCTGACCTATCTGTATATTCAATATGATGAGTTTGATAATGCTGCAACCACCATCATGAATCATTCCCCTGAAGCCTGGGATCACATGCAGTTCAAAGACGTGGCAGTTAAAGTTGCTAACGTGGAGCTCTATTATAAGGCTGTGCACTTCTACTTGCAAGAGCATCCGGACCTCATTAATGATATTCTAAATGTGCTTGCACTTCGTGTAGATCATACACGTGTTGTTGACATCATGCGGAAGGCTGGTCATTTGCTTCTTGTGAAGCCATACATGGTTGCAGTTCAGAGCAACAATGTCTCTGCTGTGAACGAGGCTTTGAACGAGATATATGTTGAGGAGGAAGACTATGAAAGATTGCGTGAATCCATTGATTTGCATGATAGCTTTGACCAAATAGGCCTTGCTCAAAAGATTGAGAAACATGAGCTTCTGGAAATGAGACGTGTTGCTGCATATATCTACAAGAAAGCTGGAAGGTGGAAGCAGTCCATTGCATTGTCAAAGAAAGACAAACTTTACAAAGATGCCATGGAGACAGCCTCACAATCTGGTGACCGTGAACTGGCAGAGGAGTTGCTTGTTTACTTCATTGAACAGGGAAAGAAAGAATGCTTTGCATCGTGCCTCTTTGTCTGTTACGATTTGATAAGGCCAGACATTGCCCTTGAACTTGCATGGATGAACAACATGATCGACTTTGCTTTCCCGTATCTGTTACAGTTTATCCGGGAATACACTGGCAAGGTTGATGAACTGGTTAAGGACAGAATTGAAGCTCAAAAGGAGGTCAAGGCTAAAGAACAGGAAGATAAAGAAGTTATTGCGCAGCAGAACATGTATGCTCAGTTGCTGCCTCTTGCTTTGCCTGCCCCACCAATGCCTGGCATGGGAGGCGGTATGGGAGGAGGGTATGCTCCACCACCACCTATGGGAGGAATGCCACCGATGGGCATGCCGCCTTTTGGCATGCCACCAATGGGCAGTAGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1088

Amino Acids

124.4

Weight (kDa)

5.04

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin PF00637 1 - 74 5.6e-09 Region in Clathrin and VPS
Clathrin PF00637 83 - 223 3.5e-27 Region in Clathrin and VPS
Clathrin PF00637 232 - 366 7e-33 Region in Clathrin and VPS
Clathrin PF00637 375 - 513 1.3e-31 Region in Clathrin and VPS
Clathrin PF00637 526 - 663 1.8e-30 Region in Clathrin and VPS
Clathrin PF00637 672 - 814 5.8e-28 Region in Clathrin and VPS
Clathrin PF00637 820 - 961 2e-32 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2234, 2403
Acc16I TGCGCA 1 cut(s) 3096
Acc36I ACCTGC 3 cut(s) 229, 1275, 1354
AccB7I CCANNNNNTGG 3 cut(s) 1600, 1645, 3152
AccII CGCG 1 cut(s) 1614
AccIII TCCGGA 1 cut(s) 2434
AciI CCGC 7 cut(s) 846, 952, 1157, 1281, 2506, 3165, 3227
AclWI GGATC 5 cut(s) 353, 366, 920, 1289, 2355
AcsI RAATTY 1 cut(s) 1751
AcuI CTGAAG 4 cut(s) 209, 684, 1225, 2358
AcvI CACGTG 1 cut(s) 2489
AdeI CACNNNGTG 1 cut(s) 2474
AfaI GTAC 5 cut(s) 380, 563, 807, 1085, 1637
AflII CTTAAG 1 cut(s) 211
AflIII ACRYGT 6 cut(s) 100, 808, 2486, 2488, 2713, 3105
AjiI CACGTC 3 cut(s) 503, 2368, 2714
AjnI CCWGG 5 cut(s) 613, 933, 1515, 2342, 3151
AleI CACNNNNGTG 1 cut(s) 1880
Alw21I GWGCWC 6 cut(s) 350, 1707, 2125, 2193, 2397, 2415
Alw26I GTCTC 4 cut(s) 2012, 2572, 2704, 2798
Alw44I GTGCAC 1 cut(s) 2411
AlwI GGATC 5 cut(s) 353, 366, 920, 1289, 2355
AlwNI CAGNNNCTG 1 cut(s) 3121
Aor13HI TCCGGA 1 cut(s) 2434
AoxI GGCC 2 cut(s) 2668, 2921
ApaLI GTGCAC 1 cut(s) 2411
ApeKI GCWGC 9 cut(s) 389, 392, 1826, 1916, 2251, 2309, 2720, 3097, 3121
ApoI RAATTY 1 cut(s) 1751
ArsI GACNNNNNNTTYG 2 cut(s) 1168, 1200
AseI ATTAAT 4 cut(s) 513, 981, 1320, 2445
Asp700I GAANNNNTTC 2 cut(s) 470, 1755
AspLEI GCGC 2 cut(s) 1616, 3097
AspS9I GGNCC 2 cut(s) 1343, 2437
AsuC2I CCSGG 3 cut(s) 1142, 1538, 2999
AsuHPI GGTGA 7 cut(s) 541, 619, 1561, 1660, 1690, 1805, 2834
AvaII GGWCC 2 cut(s) 1343, 2437
BaeGI GKGCMC 2 cut(s) 662, 2415
BamHI GGATCC 1 cut(s) 358
BanII GRGCYC 6 cut(s) 350, 720, 1142, 1689, 1707, 2397
BauI CACGAG 1 cut(s) 894
BbrPI CACGTG 1 cut(s) 2489
BbsI GAAGAC 3 cut(s) 467, 1096, 2619
Bbv12I GWGCWC 6 cut(s) 350, 1707, 2125, 2193, 2397, 2415
BbvCI CCTCAGC 1 cut(s) 1977
BbvI GCAGC 9 cut(s) 379, 401, 1813, 1928, 2263, 2296, 2707, 3108, 3109
BccI CCATC 7 cut(s) 1033, 1292, 1306, 1334, 1657, 2327, 3211
BceAI ACGGC 1 cut(s) 939
BcgI CGANNNNNNTGC 4 cut(s) 556, 590, 3205, 3239
BciT130I CCWGG 5 cut(s) 615, 935, 1517, 2344, 3153
BclI TGATCA 2 cut(s) 274, 1102
BcnI CCSGG 3 cut(s) 1142, 1538, 2999
BcoDI GTCTC 4 cut(s) 2012, 2572, 2704, 2798
BfaI CTAG 3 cut(s) 432, 647, 2163
BfmI CTRYAG 2 cut(s) 939, 1347
BfrI CTTAAG 1 cut(s) 211
BfuAI ACCTGC 3 cut(s) 229, 1275, 1354
BglI GCCNNNNNGGC 1 cut(s) 1289
BglII AGATCT 3 cut(s) 193, 544, 1048
BlpI GCTNAGC 1 cut(s) 1530
Bme1390I CCNGG 8 cut(s) 615, 935, 1142, 1517, 1538, 2344, 2999, 3153
Bme18I GGWCC 2 cut(s) 1343, 2437
BmgBI CACGTC 3 cut(s) 503, 2368, 2714
BmgT120I GGNCC 2 cut(s) 1343, 2437
BmiI GGNNCC 3 cut(s) 360, 717, 1514
BmrFI CCNGG 8 cut(s) 615, 935, 1142, 1517, 1538, 2344, 2999, 3153
BmrI ACTGGG 1 cut(s) 1867
BmuI ACTGGG 1 cut(s) 1867
BpiI GAAGAC 3 cut(s) 467, 1096, 2619
BplI GAGNNNNNCTC 2 cut(s) 2795, 2827
Bpu10I CCTNAGC 3 cut(s) 246, 1977, 2226
Bpu1102I GCTNAGC 1 cut(s) 1530
BpuEI CTTGAG 1 cut(s) 334
BpuMI CCSGG 3 cut(s) 1142, 1538, 2999
BsaAI YACGTR 1 cut(s) 2489
BsaBI GATNNNNATC 1 cut(s) 1047
BsaI GGTCTC 1 cut(s) 2012
BsaJI CCNNGG 8 cut(s) 613, 614, 933, 955, 1524, 1689, 2343, 2799
BsaWI WCCGGW 1 cut(s) 2434
Bse1I ACTGG 6 cut(s) 406, 710, 1862, 2838, 3013, 3030
Bse3DI GCAATG 3 cut(s) 701, 2760, 2928
Bse8I GATNNNNATC 1 cut(s) 1047
BseAI TCCGGA 1 cut(s) 2434
BseBI CCWGG 5 cut(s) 615, 935, 1517, 2344, 3153
BseDI CCNNGG 8 cut(s) 613, 614, 933, 955, 1524, 1689, 2343, 2799
BseGI GGATG 9 cut(s) 1044, 1048, 1452, 1609, 1630, 2035, 2075, 2431, 2955
BseJI GATNNNNATC 1 cut(s) 1047
BseMI GCAATG 3 cut(s) 701, 2760, 2928
BseNI ACTGG 6 cut(s) 406, 710, 1862, 2838, 3013, 3030
BseRI GAGGAG 4 cut(s) 1994, 2621, 2855, 3189
BseSI GKGCMC 2 cut(s) 662, 2415
BseXI GCAGC 9 cut(s) 379, 401, 1813, 1928, 2263, 2296, 2707, 3108, 3109
BsgI GTGCAG 2 cut(s) 257, 1599
Bsh1236I CGCG 1 cut(s) 1614
BshFI GGCC 2 cut(s) 2670, 2923
BsiHKAI GWGCWC 6 cut(s) 350, 1707, 2125, 2193, 2397, 2415
BsiSI CCGG 4 cut(s) 1142, 1537, 2435, 2998
BslFI GGGAC 1 cut(s) 651
BsmAI GTCTC 4 cut(s) 2012, 2572, 2704, 2798
BsmBI CGTCTC 1 cut(s) 2704
BsmFI GGGAC 1 cut(s) 651
BsmI GAATGC 3 cut(s) 625, 2885, 3213
BsnI GGCC 2 cut(s) 2670, 2923
Bso31I GGTCTC 1 cut(s) 2012
Bsp13I TCCGGA 1 cut(s) 2434
Bsp1407I TGTACA 2 cut(s) 561, 805
Bsp1720I GCTNAGC 1 cut(s) 1530
Bsp19I CCATGG 1 cut(s) 2799
BspACI CCGC 7 cut(s) 846, 952, 1157, 1281, 2506, 3165, 3227
BspANI GGCC 2 cut(s) 2670, 2923
BspEI TCCGGA 1 cut(s) 2434
BspFNI CGCG 1 cut(s) 1614
BspHI TCATGA 2 cut(s) 1173, 2322
BspLI GGNNCC 3 cut(s) 360, 717, 1514
BspMAI CTGCAG 1 cut(s) 1351
BspMI ACCTGC 3 cut(s) 229, 1275, 1354
BspPI GGATC 5 cut(s) 353, 366, 920, 1289, 2355
BspTI CTTAAG 1 cut(s) 211
BspTNI GGTCTC 1 cut(s) 2012
BsrDI GCAATG 3 cut(s) 701, 2760, 2928
BsrGI TGTACA 2 cut(s) 561, 805
BsrI ACTGG 6 cut(s) 406, 710, 1862, 2838, 3013, 3030
BssECI CCNNGG 8 cut(s) 613, 614, 933, 955, 1524, 1689, 2343, 2799
BssSI CACGAG 1 cut(s) 894
BssT1I CCWWGG 3 cut(s) 1524, 1689, 2799
Bst2BI CACGAG 1 cut(s) 894
Bst2UI CCWGG 5 cut(s) 615, 935, 1517, 2344, 3153
Bst6I CTCTTC 1 cut(s) 2034
BstAFI CTTAAG 1 cut(s) 211
BstAPI GCANNNNNTGC 2 cut(s) 1267, 1586
BstAUI TGTACA 2 cut(s) 561, 805
BstBAI YACGTR 1 cut(s) 2489
BstC8I GCNNGC 7 cut(s) 66, 1017, 1737, 2466, 3138, 3224, 3239
BstDSI CCRYGG 2 cut(s) 955, 2799
BstEII GGTNACC 1 cut(s) 2822
BstENI CCTNNNNNAGG 2 cut(s) 725, 3201
BstF5I GGATG 9 cut(s) 1044, 1048, 1452, 1609, 1630, 2035, 2075, 2431, 2955
BstFNI CGCG 1 cut(s) 1614
BstHHI GCGC 2 cut(s) 1616, 3097
BstMAI GTCTC 4 cut(s) 2012, 2572, 2704, 2798
BstNI CCWGG 5 cut(s) 615, 935, 1517, 2344, 3153
BstNSI RCATGY 7 cut(s) 122, 812, 2244, 2356, 3109, 3226, 3241
BstPI GGTNACC 1 cut(s) 2822
BstSCI CCNGG 8 cut(s) 613, 933, 1140, 1515, 1536, 2342, 2997, 3151
BstSFI CTRYAG 2 cut(s) 939, 1347
BstSLI GKGCMC 2 cut(s) 662, 2415
BstUI CGCG 1 cut(s) 1614
BstV1I GCAGC 9 cut(s) 379, 401, 1813, 1928, 2263, 2296, 2707, 3108, 3109
BstV2I GAAGAC 3 cut(s) 467, 1096, 2619
BstX2I RGATCY 5 cut(s) 193, 358, 544, 1048, 1294
BstXI CCANNNNNNTGG 1 cut(s) 1790
BstYI RGATCY 5 cut(s) 193, 358, 544, 1048, 1294
BsuRI GGCC 2 cut(s) 2670, 2923
BtgI CCRYGG 2 cut(s) 955, 2799
BtgZI GCGATG 1 cut(s) 1569
BtrI CACGTC 3 cut(s) 503, 2368, 2714
BtsCI GGATG 9 cut(s) 1044, 1048, 1452, 1609, 1630, 2035, 2075, 2431, 2955
BtsIMutI CAGTG 6 cut(s) 284, 717, 1249, 1705, 2053, 3006
BveI ACCTGC 3 cut(s) 229, 1275, 1354
Cac8I GCNNGC 7 cut(s) 66, 1017, 1737, 2466, 3138, 3224, 3239
CaiI CAGNNNCTG 1 cut(s) 3121
CciI TCATGA 2 cut(s) 1173, 2322
CfoI GCGC 2 cut(s) 1616, 3097
Cfr13I GGNCC 2 cut(s) 1343, 2437
Csp6I GTAC 5 cut(s) 379, 562, 806, 1084, 1636
CspCI CAANNNNNGTGG 2 cut(s) 1008, 1043
CviQI GTAC 5 cut(s) 379, 562, 806, 1084, 1636
DraIII CACNNNGTG 1 cut(s) 2474
Eam1104I CTCTTC 1 cut(s) 2034
EarI CTCTTC 1 cut(s) 2034
Ecl136II GAGCTC 3 cut(s) 348, 1705, 2395
Eco130I CCWWGG 3 cut(s) 1524, 1689, 2799
Eco147I AGGCCT 1 cut(s) 2670
Eco24I GRGCYC 6 cut(s) 350, 720, 1142, 1689, 1707, 2397
Eco31I GGTCTC 1 cut(s) 2012
Eco32I GATATC 2 cut(s) 367, 2168
Eco47I GGWCC 2 cut(s) 1343, 2437
Eco53kI GAGCTC 3 cut(s) 348, 1705, 2395
Eco57I CTGAAG 4 cut(s) 209, 684, 1225, 2358
Eco72I CACGTG 1 cut(s) 2489
Eco91I GGTNACC 1 cut(s) 2822
EcoICRI GAGCTC 3 cut(s) 348, 1705, 2395
EcoNI CCTNNNNNAGG 2 cut(s) 725, 3201
EcoO65I GGTNACC 1 cut(s) 2822
EcoRII CCWGG 5 cut(s) 613, 933, 1515, 2342, 3151
EcoRV GATATC 2 cut(s) 367, 2168
EcoT14I CCWWGG 3 cut(s) 1524, 1689, 2799
EcoT22I ATGCAT 1 cut(s) 1717
EcoT38I GRGCYC 6 cut(s) 350, 720, 1142, 1689, 1707, 2397
ErhI CCWWGG 3 cut(s) 1524, 1689, 2799
Esp3I CGTCTC 1 cut(s) 2704
FalI AAGNNNNNCTT 8 cut(s) 458, 490, 986, 1018, 1400, 1432, 2769, 2801
FaqI GGGAC 1 cut(s) 651
FauNDI CATATG 2 cut(s) 310, 1717
FbaI TGATCA 2 cut(s) 274, 1102
FokI GGATG 9 cut(s) 1051, 1055, 1439, 1616, 1637, 2042, 2082, 2418, 2962
FriOI GRGCYC 6 cut(s) 350, 720, 1142, 1689, 1707, 2397
FspBI CTAG 3 cut(s) 432, 647, 2163
FspI TGCGCA 1 cut(s) 3096
GlaI GCGC 2 cut(s) 1615, 3096
HaeIII GGCC 2 cut(s) 2670, 2923
HapII CCGG 4 cut(s) 1142, 1537, 2435, 2998
HhaI GCGC 2 cut(s) 1616, 3097
Hin6I GCGC 2 cut(s) 1614, 3095
HinP1I GCGC 2 cut(s) 1614, 3095
HincII GTYRAC 2 cut(s) 208, 2497
HindII GTYRAC 2 cut(s) 208, 2497
HindIII AAGCTT 1 cut(s) 2228
HinfI GANTC 7 cut(s) 437, 745, 890, 1565, 2133, 2326, 2633
HpaII CCGG 4 cut(s) 1142, 1537, 2435, 2998
HphI GGTGA 7 cut(s) 541, 619, 1561, 1660, 1690, 1805, 2834
Hpy188I TCNGA 9 cut(s) 82, 228, 259, 603, 1457, 1621, 1662, 1998, 2556
HpyCH4IV ACGT 9 cut(s) 100, 502, 597, 922, 2119, 2367, 2388, 2488, 2713
HpySE526I ACGT 9 cut(s) 100, 502, 597, 922, 2119, 2367, 2388, 2488, 2713
HspAI GCGC 2 cut(s) 1614, 3095
Kpn2I TCCGGA 1 cut(s) 2434
Ksp22I TGATCA 2 cut(s) 274, 1102
Lsp1109I GCAGC 9 cut(s) 379, 401, 1813, 1928, 2263, 2296, 2707, 3108, 3109
MaeI CTAG 3 cut(s) 432, 647, 2163
MaeII ACGT 9 cut(s) 100, 502, 597, 922, 2119, 2367, 2388, 2488, 2713
MflI RGATCY 5 cut(s) 193, 358, 544, 1048, 1294
MlyI GAGTC 1 cut(s) 1574
Mph1103I ATGCAT 1 cut(s) 1717
MroI TCCGGA 1 cut(s) 2434
MroXI GAANNNNTTC 2 cut(s) 470, 1755
MslI CAYNNNNRTG 4 cut(s) 181, 1880, 2321, 2487
MspA1I CMGCKG 1 cut(s) 392
MspCI CTTAAG 1 cut(s) 211
MspI CCGG 4 cut(s) 1142, 1537, 2435, 2998
MspR9I CCNGG 8 cut(s) 615, 935, 1142, 1517, 1538, 2344, 2999, 3153
Mva1269I GAATGC 3 cut(s) 625, 2885, 3213
MvaI CCWGG 5 cut(s) 615, 935, 1517, 2344, 3153
MvnI CGCG 1 cut(s) 1614
NciI CCSGG 3 cut(s) 1142, 1538, 2999
NcoI CCATGG 1 cut(s) 2799
NdeI CATATG 2 cut(s) 310, 1717
NlaIV GGNNCC 3 cut(s) 360, 717, 1514
NmuCI GTSAC 5 cut(s) 135, 965, 985, 1873, 2822
NsbI TGCGCA 1 cut(s) 3096
NsiI ATGCAT 1 cut(s) 1717
NspI RCATGY 7 cut(s) 122, 812, 2244, 2356, 3109, 3226, 3241
OliI CACNNNNGTG 1 cut(s) 1880
PaeI GCATGC 2 cut(s) 3226, 3241
PagI TCATGA 2 cut(s) 1173, 2322
PasI CCCWGGG 1 cut(s) 614
PceI AGGCCT 1 cut(s) 2670
PciI ACATGT 2 cut(s) 808, 3105
PctI GAATGC 3 cut(s) 625, 2885, 3213
PdmI GAANNNNTTC 2 cut(s) 470, 1755
PfeI GAWTC 6 cut(s) 437, 745, 890, 2133, 2326, 2633
PflMI CCANNNNNTGG 3 cut(s) 1600, 1645, 3152
PfoI TCCNGGA 2 cut(s) 1536, 2997
PleI GAGTC 1 cut(s) 1573
PmaCI CACGTG 1 cut(s) 2489
PmlI CACGTG 1 cut(s) 2489
PpsI GAGTC 1 cut(s) 1573
Ppu21I YACGTR 1 cut(s) 2489
PscI ACATGT 2 cut(s) 808, 3105
PshBI ATTAAT 4 cut(s) 513, 981, 1320, 2445
PsiI TTATAA 2 cut(s) 2234, 2403
Psp124BI GAGCTC 3 cut(s) 350, 1707, 2397
Psp6I CCWGG 5 cut(s) 613, 933, 1515, 2342, 3151
PspCI CACGTG 1 cut(s) 2489
PspEI GGTNACC 1 cut(s) 2822
PspGI CCWGG 5 cut(s) 613, 933, 1515, 2342, 3151
PspN4I GGNNCC 3 cut(s) 360, 717, 1514
PspPI GGNCC 2 cut(s) 1343, 2437
PstI CTGCAG 1 cut(s) 1351
PstNI CAGNNNCTG 1 cut(s) 3121
PsuI RGATCY 5 cut(s) 193, 358, 544, 1048, 1294
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 5 cut(s) 380, 563, 807, 1085, 1637
RsaNI GTAC 5 cut(s) 379, 562, 806, 1084, 1636
RseI CAYNNNNRTG 4 cut(s) 181, 1880, 2321, 2487
SacI GAGCTC 3 cut(s) 350, 1707, 2397
Sau96I GGNCC 2 cut(s) 1343, 2437
SchI GAGTC 1 cut(s) 1574
ScrFI CCNGG 8 cut(s) 615, 935, 1142, 1517, 1538, 2344, 2999, 3153
SfcI CTRYAG 2 cut(s) 939, 1347
SinI GGWCC 2 cut(s) 1343, 2437
SmiMI CAYNNNNRTG 4 cut(s) 181, 1880, 2321, 2487
SmlI CTYRAG 2 cut(s) 211, 349
SmoI CTYRAG 2 cut(s) 211, 349
SphI GCATGC 2 cut(s) 3226, 3241
SseBI AGGCCT 1 cut(s) 2670
SsiI CCGC 7 cut(s) 846, 952, 1157, 1281, 2506, 3165, 3227
SspI AATATT 2 cut(s) 911, 2221
SspMI CTAG 3 cut(s) 432, 647, 2163
SstI GAGCTC 3 cut(s) 350, 1707, 2397
StuI AGGCCT 1 cut(s) 2670
StyD4I CCNGG 8 cut(s) 613, 933, 1140, 1515, 1536, 2342, 2997, 3151
StyI CCWWGG 3 cut(s) 1524, 1689, 2799
TaiI ACGT 9 cut(s) 103, 505, 600, 925, 2122, 2370, 2391, 2491, 2716
TaqI TCGA 2 cut(s) 440, 2964
TatI WGTACW 4 cut(s) 378, 561, 805, 1083
TauI GCSGC 3 cut(s) 849, 1160, 3229
TfiI GAWTC 6 cut(s) 437, 745, 890, 2133, 2326, 2633
TscAI CASTG 6 cut(s) 284, 717, 1249, 1705, 2053, 3013
TseFI GTSAC 5 cut(s) 135, 965, 985, 1873, 2822
TseI GCWGC 9 cut(s) 389, 392, 1826, 1916, 2251, 2309, 2720, 3097, 3121
Tsp45I GTSAC 5 cut(s) 135, 965, 985, 1873, 2822
TspGWI ACGGA 4 cut(s) 686, 1478, 1565, 1976
TspRI CASTG 6 cut(s) 284, 717, 1249, 1705, 2053, 3013
Van91I CCANNNNNTGG 3 cut(s) 1600, 1645, 3152
Vha464I CTTAAG 1 cut(s) 211
VneI GTGCAC 1 cut(s) 2411
VpaK11BI GGWCC 2 cut(s) 1343, 2437
VspI ATTAAT 4 cut(s) 513, 981, 1320, 2445
XagI CCTNNNNNAGG 2 cut(s) 725, 3201
XapI RAATTY 1 cut(s) 1751
XceI RCATGY 7 cut(s) 122, 812, 2244, 2356, 3109, 3226, 3241
XcmI CCANNNNNNNNNTGG 1 cut(s) 1597
XmnI GAANNNNTTC 2 cut(s) 470, 1755
XspI CTAG 3 cut(s) 432, 647, 2163
Zsp2I ATGCAT 1 cut(s) 1717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.