pycom02g09440

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003928
Physical Location & Seq
Forward (+)
6530446 .. 6538085
7640 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1047 bp
ATGTTCATCGTCAGTGAAAAGGTTGGCTTGATTTTACACCGACTTCTGTCATTGGATTCTTCTCACACGATCCAGCTTTCTCCCACGCGTGTCAAACTCACAACGGCTATATTTTCCTTTTACAGGCCCAATTGCAGCGCAAAGGTCGGTCATCAAGTGGGCCCCGCAGAGGATCTGCTGTATAATTCCATATCTTGTTTCCCATTCCAATTCAGGCGACCGTTAAAAACCCAAAAAAAAAAAAAAAACCATCTTTCTCTCTCTGTGACCATGACGGTCGCCGCCGCAGCCACCACCCACACCAAGAAGCTGGTGAAGCTCGGCCGCTCTCTCTTCCGATGGGGATTCAACTCATCCAAATGCAAAATGCAAGCGAAGATGGCGGTGGCCAGAATAAAGCTGCTGAGGAACAAGAGGCAGGCGGTGGTGAAGCAGATGAGGCGTGACATCGCCTTACTCCTACAATCTGGTCAAGACGCCACTGCTCGAATCCGGGTTGAACATGTGATCAGGGAACAGAACATTTTGGCTGCCAACGAGTTCATTGAGCTCTTTTGTGAATTAGTAGTATCTAGACTTTCTATCATCGCAAAGCAAAGGGAGTGTCCGCCTGATCTCAAAGAAGGGATTGCTAGTTTAATCTTTGCCTCCCCCAGGTGCTCTGAGATTCCTGAACTAATTTCCCTAAGGAATATTTTTGAGAAAAAGTATGGTACAGATTTTGTATCTGCGGCTGTTGATGTACGTCCCAGCTGTGGTGTGAATCGCATGCTGATTGACAAGCTCTCTGTTAGAACCCCTACGGGTGAAGTAAAGTTGAAACTCCTCAAGGAGATAGCCAAGGAATACCAGGTTGAATGGGATACATCGGAATCTGAACAGGAACTTCTCAAGCCCCCAGAAGAGCTTATACAAGGACCATGCAATTTTGTTAGTGCTACCAGCATGCCTGTGCACAATGTGCAGCCTCAATCTGTTGAGACTAATAAGCCAGACACTAGTTCTTCACACAGAACAAGTGGTGGTGGAGAACGCAGAACCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

349

Amino Acids

39.11

Weight (kDa)

9.81

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 122 - 286 5.8e-62 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 327
AccII CGCG 1 cut(s) 88
AciI CCGC 8 cut(s) 165, 282, 285, 325, 383, 422, 608, 731
AclWI GGATC 2 cut(s) 64, 180
AcoI YGGCCR 2 cut(s) 322, 387
AcyI GRCGYC 1 cut(s) 477
AdeI CACNNNGTG 1 cut(s) 961
AfaI GTAC 2 cut(s) 715, 744
AfiI CCNNNNNNNGG 4 cut(s) 123, 169, 654, 755
AflIII ACRYGT 2 cut(s) 86, 502
AgsI TTSAA 4 cut(s) 349, 500, 820, 857
AhlI ACTAGT 1 cut(s) 998
AjnI CCWGG 2 cut(s) 653, 849
AluBI AGCT 8 cut(s) 76, 310, 319, 400, 550, 753, 784, 907
AluI AGCT 8 cut(s) 76, 310, 319, 400, 550, 753, 784, 907
Alw21I GWGCWC 3 cut(s) 552, 662, 957
Alw26I GTCTC 1 cut(s) 974
Alw44I GTGCAC 1 cut(s) 953
AlwI GGATC 2 cut(s) 64, 180
AoxI GGCC 4 cut(s) 125, 160, 322, 387
ApaI GGGCCC 1 cut(s) 164
ApaLI GTGCAC 1 cut(s) 953
ApeKI GCWGC 5 cut(s) 135, 287, 400, 530, 964
ArsI GACNNNNNNTTYG 2 cut(s) 589, 621
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 4 cut(s) 126, 160, 161, 917
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 3 cut(s) 325, 439, 818
AvaII GGWCC 1 cut(s) 917
AxyI CCTNAGG 1 cut(s) 686
BaeGI GKGCMC 2 cut(s) 164, 957
BalI TGGCCA 1 cut(s) 389
BanII GRGCYC 2 cut(s) 164, 552
BarI GAAGNNNNNNTAC 2 cut(s) 894, 926
Bbv12I GWGCWC 3 cut(s) 552, 662, 957
BbvCI CCTCAGC 1 cut(s) 404
BbvI GCAGC 5 cut(s) 147, 299, 387, 517, 976
BccI CCATC 3 cut(s) 258, 333, 373
BceAI ACGGC 1 cut(s) 120
BciT130I CCWGG 2 cut(s) 655, 851
BciVI GTATCC 1 cut(s) 856
BclI TGATCA 1 cut(s) 507
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 1 cut(s) 974
BcuI ACTAGT 1 cut(s) 998
BfaI CTAG 3 cut(s) 573, 633, 999
BfuI GTATCC 1 cut(s) 856
BisI GCNGC 9 cut(s) 136, 282, 285, 288, 325, 401, 531, 732, 965
BlsI GCNGC 9 cut(s) 137, 283, 286, 289, 326, 402, 532, 733, 966
Bme1390I CCNGG 3 cut(s) 494, 655, 851
Bme18I GGWCC 1 cut(s) 917
BmgT120I GGNCC 4 cut(s) 126, 160, 161, 917
BmiI GGNNCC 2 cut(s) 162, 163
BmrFI CCNGG 3 cut(s) 494, 655, 851
BoxI GACNNNNGTC 1 cut(s) 46
Bpu10I CCTNAGC 1 cut(s) 404
BpuEI CTTGAG 2 cut(s) 812, 875
BpuMI CCSGG 1 cut(s) 494
BsaHI GRCGYC 1 cut(s) 477
BsaJI CCNNGG 2 cut(s) 653, 840
Bsc4I CCNNNNNNNGG 4 cut(s) 123, 169, 654, 755
Bse21I CCTNAGG 1 cut(s) 686
BseBI CCWGG 2 cut(s) 655, 851
BseDI CCNNGG 2 cut(s) 653, 840
BseGI GGATG 1 cut(s) 353
BseLI CCNNNNNNNGG 4 cut(s) 123, 169, 654, 755
BseMII CTCAG 2 cut(s) 395, 654
BseRI GAGGAG 1 cut(s) 815
BseSI GKGCMC 2 cut(s) 164, 957
BseX3I CGGCCG 1 cut(s) 322
BseXI GCAGC 5 cut(s) 147, 299, 387, 517, 976
BseYI CCCAGC 1 cut(s) 749
BsgI GTGCAG 1 cut(s) 983
Bsh1236I CGCG 1 cut(s) 88
Bsh1285I CGRYCG 3 cut(s) 221, 279, 325
BshFI GGCC 4 cut(s) 127, 162, 324, 389
BsiEI CGRYCG 3 cut(s) 221, 279, 325
BsiHKAI GWGCWC 3 cut(s) 552, 662, 957
BsiSI CCGG 1 cut(s) 493
BslFI GGGAC 1 cut(s) 732
BslI CCNNNNNNNGG 4 cut(s) 123, 169, 654, 755
BsmAI GTCTC 1 cut(s) 974
BsmFI GGGAC 1 cut(s) 732
BsnI GGCC 4 cut(s) 127, 162, 324, 389
Bsp120I GGGCCC 1 cut(s) 160
Bsp1286I GDGCHC 4 cut(s) 164, 552, 662, 957
Bsp143I GATC 4 cut(s) 69, 172, 507, 613
BspACI CCGC 8 cut(s) 165, 282, 285, 325, 383, 422, 608, 731
BspANI GGCC 4 cut(s) 127, 162, 324, 389
BspCNI CTCAG 2 cut(s) 396, 655
BspFNI CGCG 1 cut(s) 88
BspLI GGNNCC 2 cut(s) 162, 163
BspPI GGATC 2 cut(s) 64, 180
BspQI GCTCTTC 1 cut(s) 897
BsrBI CCGCTC 1 cut(s) 327
BssECI CCNNGG 2 cut(s) 653, 840
BssMI GATC 4 cut(s) 69, 172, 507, 613
BssNI GRCGYC 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 840
Bst2UI CCWGG 2 cut(s) 655, 851
Bst4CI ACNGT 2 cut(s) 222, 277
Bst6I CTCTTC 2 cut(s) 338, 897
BstACI GRCGYC 1 cut(s) 477
BstAPI GCANNNNNTGC 1 cut(s) 961
BstC8I GCNNGC 4 cut(s) 372, 420, 770, 947
BstDEI CTNAG 3 cut(s) 404, 663, 686
BstENI CCTNNNNNAGG 2 cut(s) 121, 652
BstF5I GGATG 1 cut(s) 353
BstFNI CGCG 1 cut(s) 88
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 4 cut(s) 72, 175, 510, 616
BstMAI GTCTC 1 cut(s) 974
BstMBI GATC 4 cut(s) 69, 172, 507, 613
BstMCI CGRYCG 3 cut(s) 221, 279, 325
BstMWI GCNNNNNNNGC 5 cut(s) 287, 316, 380, 439, 961
BstNI CCWGG 2 cut(s) 655, 851
BstNSI RCATGY 3 cut(s) 506, 772, 949
BstPAI GACNNNNGTC 1 cut(s) 46
BstSCI CCNGG 3 cut(s) 492, 653, 849
BstSLI GKGCMC 2 cut(s) 164, 957
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 5 cut(s) 147, 299, 387, 517, 976
BstX2I RGATCY 1 cut(s) 172
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 1 cut(s) 172
BstZI CGGCCG 1 cut(s) 322
Bsu36I CCTNAGG 1 cut(s) 686
BsuI GTATCC 1 cut(s) 856
BsuRI GGCC 4 cut(s) 127, 162, 324, 389
BtgZI GCGATG 2 cut(s) 433, 571
BtsCI GGATG 1 cut(s) 353
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 2 cut(s) 19, 480
Cac8I GCNNGC 4 cut(s) 372, 420, 770, 947
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 4 cut(s) 126, 160, 161, 917
CseI GACGC 1 cut(s) 485
CsiI ACCWGGT 1 cut(s) 849
Csp6I GTAC 2 cut(s) 714, 743
CviAII CATG 5 cut(s) 271, 503, 769, 921, 946
CviQI GTAC 2 cut(s) 714, 743
DdeI CTNAG 3 cut(s) 404, 663, 686
DpnI GATC 4 cut(s) 71, 174, 509, 615
DpnII GATC 4 cut(s) 69, 172, 507, 613
DraIII CACNNNGTG 1 cut(s) 961
EaeI YGGCCR 2 cut(s) 322, 387
EagI CGGCCG 1 cut(s) 322
Eam1104I CTCTTC 2 cut(s) 338, 897
EarI CTCTTC 2 cut(s) 338, 897
EciI GGCGGA 1 cut(s) 597
Ecl136II GAGCTC 1 cut(s) 550
EclXI CGGCCG 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 840
Eco24I GRGCYC 2 cut(s) 164, 552
Eco47I GGWCC 1 cut(s) 917
Eco52I CGGCCG 1 cut(s) 322
Eco53kI GAGCTC 1 cut(s) 550
Eco81I CCTNAGG 1 cut(s) 686
EcoICRI GAGCTC 1 cut(s) 550
EcoNI CCTNNNNNAGG 2 cut(s) 121, 652
EcoO109I RGGNCCY 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 653, 849
EcoT14I CCWWGG 1 cut(s) 840
EcoT38I GRGCYC 2 cut(s) 164, 552
ErhI CCWWGG 1 cut(s) 840
FaeI CATG 5 cut(s) 274, 506, 772, 924, 949
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 1 cut(s) 732
FatI CATG 5 cut(s) 270, 502, 768, 920, 945
FauI CCCGC 1 cut(s) 172
FbaI TGATCA 1 cut(s) 507
Fnu4HI GCNGC 9 cut(s) 136, 282, 285, 288, 325, 401, 531, 732, 965
FokI GGATG 1 cut(s) 340
FriOI GRGCYC 2 cut(s) 164, 552
Fsp4HI GCNGC 9 cut(s) 136, 282, 285, 288, 325, 401, 531, 732, 965
FspBI CTAG 3 cut(s) 573, 633, 999
GlaI GCGC 1 cut(s) 139
GluI GCNGC 9 cut(s) 136, 282, 285, 288, 325, 401, 531, 732, 965
GsaI CCCAGC 1 cut(s) 753
HaeIII GGCC 4 cut(s) 127, 162, 324, 389
HapII CCGG 1 cut(s) 493
HgaI GACGC 1 cut(s) 485
HhaI GCGC 1 cut(s) 140
Hin1I GRCGYC 1 cut(s) 477
Hin1II CATG 5 cut(s) 274, 506, 772, 924, 949
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HinfI GANTC 6 cut(s) 56, 345, 489, 667, 763, 872
HpaII CCGG 1 cut(s) 493
HphI GGTGA 3 cut(s) 325, 439, 818
Hpy166II GTNNAC 1 cut(s) 955
Hpy188I TCNGA 4 cut(s) 338, 664, 871, 877
Hpy188III TCNNGA 3 cut(s) 473, 573, 671
Hpy8I GTNNAC 1 cut(s) 955
HpyAV CCTTC 1 cut(s) 617
HpyCH4III ACNGT 2 cut(s) 222, 277
HpyCH4IV ACGT 1 cut(s) 745
HpyCH4V TGCA 6 cut(s) 135, 363, 370, 924, 955, 964
HpyF10VI GCNNNNNNNGC 5 cut(s) 287, 316, 380, 439, 961
HpyF3I CTNAG 3 cut(s) 404, 663, 686
HpySE526I ACGT 1 cut(s) 745
Hsp92I GRCGYC 1 cut(s) 477
Hsp92II CATG 5 cut(s) 274, 506, 772, 924, 949
HspAI GCGC 1 cut(s) 138
Ksp22I TGATCA 1 cut(s) 507
Kzo9I GATC 4 cut(s) 69, 172, 507, 613
LguI GCTCTTC 1 cut(s) 897
Lsp1109I GCAGC 5 cut(s) 147, 299, 387, 517, 976
MabI ACCWGGT 1 cut(s) 849
MaeI CTAG 3 cut(s) 573, 633, 999
MaeII ACGT 1 cut(s) 745
MaeIII GTNAC 2 cut(s) 265, 443
MalI GATC 4 cut(s) 71, 174, 509, 615
MbiI CCGCTC 1 cut(s) 327
MboI GATC 4 cut(s) 69, 172, 507, 613
MboII GAAGA 5 cut(s) 51, 325, 388, 914, 996
MfeI CAATTG 1 cut(s) 130
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 4 cut(s) 164, 552, 662, 957
MlsI TGGCCA 1 cut(s) 389
MluCI AATT 6 cut(s) 130, 184, 209, 560, 678, 925
MluI ACGCGT 1 cut(s) 86
MluNI TGGCCA 1 cut(s) 389
MnlI CCTC 7 cut(s) 163, 399, 408, 432, 658, 836, 978
Mox20I TGGCCA 1 cut(s) 389
MscI TGGCCA 1 cut(s) 389
MseI TTAA 2 cut(s) 224, 638
MslI CAYNNNNRTG 2 cut(s) 358, 950
Msp20I TGGCCA 1 cut(s) 389
MspA1I CMGCKG 1 cut(s) 753
MspI CCGG 1 cut(s) 493
MspR9I CCNGG 3 cut(s) 494, 655, 851
MunI CAATTG 1 cut(s) 130
MvaI CCWGG 2 cut(s) 655, 851
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 5 cut(s) 287, 316, 380, 439, 961
NciI CCSGG 1 cut(s) 494
NdeII GATC 4 cut(s) 69, 172, 507, 613
NlaIII CATG 5 cut(s) 274, 506, 772, 924, 949
NlaIV GGNNCC 2 cut(s) 162, 163
NmeAIII GCCGAG 1 cut(s) 300
NmuCI GTSAC 2 cut(s) 265, 443
NspI RCATGY 3 cut(s) 506, 772, 949
PaeI GCATGC 2 cut(s) 772, 949
PciI ACATGT 1 cut(s) 502
PciSI GCTCTTC 1 cut(s) 897
PfeI GAWTC 6 cut(s) 56, 345, 489, 667, 763, 872
PkrI GCNGC 9 cut(s) 137, 283, 286, 289, 326, 402, 532, 733, 966
PscI ACATGT 1 cut(s) 502
PshAI GACNNNNGTC 1 cut(s) 46
Psp124BI GAGCTC 1 cut(s) 552
Psp6I CCWGG 2 cut(s) 653, 849
PspFI CCCAGC 1 cut(s) 749
PspGI CCWGG 2 cut(s) 653, 849
PspN4I GGNNCC 2 cut(s) 162, 163
PspOMI GGGCCC 1 cut(s) 160
PspPI GGNCC 4 cut(s) 126, 160, 161, 917
PsuI RGATCY 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 753
RsaI GTAC 2 cut(s) 715, 744
RsaNI GTAC 2 cut(s) 714, 743
RseI CAYNNNNRTG 2 cut(s) 358, 950
SacI GAGCTC 1 cut(s) 552
SapI GCTCTTC 1 cut(s) 897
SaqAI TTAA 2 cut(s) 224, 638
SatI GCNGC 9 cut(s) 136, 282, 285, 288, 325, 401, 531, 732, 965
Sau3AI GATC 4 cut(s) 69, 172, 507, 613
Sau96I GGNCC 4 cut(s) 126, 160, 161, 917
ScrFI CCNGG 3 cut(s) 494, 655, 851
SduI GDGCHC 4 cut(s) 164, 552, 662, 957
SexAI ACCWGGT 1 cut(s) 849
SinI GGWCC 1 cut(s) 917
SmiMI CAYNNNNRTG 2 cut(s) 358, 950
SmlI CTYRAG 2 cut(s) 827, 890
SmoI CTYRAG 2 cut(s) 827, 890
SpeI ACTAGT 1 cut(s) 998
SphI GCATGC 2 cut(s) 772, 949
Sse9I AATT 6 cut(s) 130, 184, 209, 560, 678, 925
SsiI CCGC 8 cut(s) 165, 282, 285, 325, 383, 422, 608, 731
SspI AATATT 1 cut(s) 694
SspMI CTAG 3 cut(s) 573, 633, 999
SstI GAGCTC 1 cut(s) 552
StyD4I CCNGG 3 cut(s) 492, 653, 849
StyI CCWWGG 1 cut(s) 840
TaaI ACNGT 2 cut(s) 222, 277
TaiI ACGT 1 cut(s) 748
TaqI TCGA 1 cut(s) 487
TaqII GACCGA 1 cut(s) 137
TasI AATT 6 cut(s) 130, 184, 209, 560, 678, 925
TauI GCSGC 4 cut(s) 284, 287, 327, 734
TfiI GAWTC 6 cut(s) 56, 345, 489, 667, 763, 872
Tru1I TTAA 2 cut(s) 224, 638
Tru9I TTAA 2 cut(s) 224, 638
TscAI CASTG 2 cut(s) 19, 487
TseFI GTSAC 2 cut(s) 265, 443
TseI GCWGC 5 cut(s) 135, 287, 400, 530, 964
Tsp45I GTSAC 2 cut(s) 265, 443
TspDTI ATGAA 1 cut(s) 532
TspRI CASTG 2 cut(s) 19, 487
VneI GTGCAC 1 cut(s) 953
VpaK11BI GGWCC 1 cut(s) 917
XagI CCTNNNNNAGG 2 cut(s) 121, 652
XbaI TCTAGA 1 cut(s) 572
XceI RCATGY 3 cut(s) 506, 772, 949
XspI CTAG 3 cut(s) 573, 633, 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.