pycom03g05230

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002740
Physical Location & Seq
Reverse (-)
4049227 .. 4059162
9936 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5301 bp
ATGCTTCAACTTACGTCTCCTCCTCTTCTGTATACATTCCCATACTACAAGATCCACACACACACACACTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCCCCCTCCCCCCTGCAAATCCACAAACAGAAGCTTCTGCTGTGCCACATTCTTCTCTGCTTATCGGGTCCTCCTATCGAAGCCCCGACCCAGGCACCATGGCCGCCGCTAACGCCCCCATGACCATGAAAGAGGCCCTCACGTTGCCTAGTATTGGGATCAATCCCCAATTCATTACGTTTACGCATGTGACTATGGAATCGGATAAGTACATTTGTGTTAGGGAGACATCACCGCAGAATAGCGTCGTTATCATCGATATGAACATGCCCATGCAGCCGTTGCGTCGACCCATTACCGCCGACTCGGCTTTGATGAATCCCAATTCCAGAATTCTTGCTCTAAAAGCTCAAGTCCAAGGAACTACTCAGGACCATTTACAAATATTTAACATCGAAATGAAAGCAAAGATGAAATCACATCAAATGCCTGAGCAGGTTGTCTTTTGGAAGTGGATCACTCCAAAGATGCTAGGTCTGGTCACTCAAACCTCAGTATATCATTGGTTAATTGAAGGTGATTCCGAGCCTACAAAGGTCTTTGAGCGGACAGCTAATTTGGCAAACAATCAAATAATCAACTACCGTTGTGATCCTTCGGAAAAGTGGTTGGTCTTAATTGGAATTGCTCCTGGTTCAGCAGAGAGGCCACAACTTGTCAAAGGAAATATGCAACTTTTTTCTGTGGAACAGAATCGTAGTCAGGCTCTTGAAGCACATGCTGCATCGTTTGCCACATTTAAAGTTCCAGGGAATGAGCATCCTTCAATTCTTATTTGTTTTGCCTCAAAGTCCTCCAATGCTGGACAGGTTACATCAAAGATGCATGTAATTGAGCTTGGTGCCCAGCCAGGAAAACCATCATATACTAAGAAGCAAGCAGATTTATTCTTTCCTCCAGATTTTGCTGACGACTTTCCAGTATCAATGCAGATATCGGAAAAGTATGGGTTGATATATGTTATCACAAAGCTTGGACTTTTATTTGTATATGACCTAGAGACAGCAACTGCAGTTTATAGAAATCGAATCAGTTCAGATCCTATATTTTTGACAGCTGAAGCTTCAACTGTTGGGGGGTTTTATGCAATCAATAGGCGAGGCCAGGTCTTACTTGCCACTATAAATGAACAAACAATTGTACCATTTGTTAGTGGTCAGTTAAACAATTTGGGGCTTGCAGTGAGTCTTGCGAAAAGAGGAAACCTTCCTGGTGCTGAGGACCTGGTTGTCCAGCGGTTCCAAGAATTGTTTTCTCAAACAAAGTATAAGGAGGCTGCAGAGCTTGCCGCAGAATCTCCAATGGGAATACTCCGTACTCCTGATACTGTTGCTAAATTTCAGAGTGTTCCTGTGCAAGCGGGGCAAACCCCACCTCTATTGCAGTATTTTGGAACTCTTTTAACTAAAGGAAAACTCAATGCTTATGAATCATTAGAACTGTCACGTTTAGTTGTAAATCAGAACAAAAAGAATCTTTTGGAGAATTGGCTAGCAGAGGACAAGCTAGAATGTAGTGAAGAGCTTGGAGATCTTGTGAAGACAGTGGATAATGACCTTGCTTTGAAAATATACATCAAAGCTCGAGCAACGCCCAAAGTTGTTGCAGCATTTGCTGAGAGAAGGGAATTTGACAAGATTTTGATATACTCAAAGCAGGTTGGATATACACCTGATTATTTGTTCCTTCTCCAAACAATTTTGCGGGCAGATCCCCAGGGGGCTGTTAATTTTGCTCTAATGATGTCTCAAATGGAGGGAGGTTGCCCCATTGACTACAATACCATCACCGATCTGTTCCTTCAGAGGAACTTGATCCGTGAAGCAACAGCCTTTTTGTTGGATGTGCTGAAGCCGAATCTACCTGAGCATGGATTCCTTCAGACAAAGGTTTTGGAAATCAATCTTGTGACTTTTCCAAATGTTGCGGATGCTATATTGGCCAATGGTATGTTCAGCCATTATGATCGATCACGCATTGGTCAACTTTGTGAAAAAGCTGGTTTGTATTTGCGTGCCCTACAGCACTACACTGAGTTGCCTGATATCAAACGTGTCATTGTGAACACACATGCAATTGACCCTCAGGCTCTTGTTGAGTTCTTTGGAACTCTTTCTGTAGAATGGGCTCTTGAGTGCATGAAAGATCTTCTGCTGGTCAATCTTAGGGGCAATCTTCAGATTATTGTACAGACTGCCAAAGAGTATTGCGAGCAATTAGGTGTAGATAAGTGTATAAAATTGTTTGAGCAGTTCAAGTCCTATGAGGGGCTGTACTTCTTCCTAGGCGCATATTTGAGCTCCAGCGAGGATCGATCTGATATTCATTTCAAGTACATTGAAGCAGCTGCAAAAACTGGACAGATTAAGGAGGTGGAGCGTGTGACACGGGAATCAAATTTTTATGACCCTGAAAAGGCTAAGAACTTTTTAATGGAAGCAAAGCTTCCAGATGCACGTCCTCTAATTAATGTCTGTGATCGTTTTGGCTTTGTTCCAGATCTCACCCATTATCTGTATATGAACAATATGCTTCGTTACATTGAAGGTTATGTCCAAAAGGTCAATCCAGGAAATGCTCCTTTAGTTGTAGGGCAGCTACTGGATGATGAATGTCCTGAAGATTTCATTAAAGGTCTTATTCTATCAGTTCGTTCTCTTCTACCGGTTGAGCCCCTTGTCGAGGAATGTGAAAAGAGGAATCGTCTTCGCTTGCTCACTCAATTTTTGGAGCATCTTGTGAGTGAGGGAAGCCAAGATGTACATGTCCACAATGCTCTGGGTAAAATCATCATAGATAGTGGCAACAATCCTGAGCATTTGCTCACAACTAACCCATACTATGATTCTCGCGTTGTGGGTAAATATTGTGAGAAAAGGGATCCTACGCTTGCTGTTGTTGCATACCGTAGAGGGCAATGTGACGATGAGCTCATTAATGTTACAAATAAAAATTCCTTGTTCAAACTCCAGGCCAGATATGTCGTGGAAAGAATGGATGATGACCTCTGGGCTAAGGTCCTTGATCCTGAAAATGAATATAAAAGGCAACTAATTGATCAAGTTGGATCTACTGCCTTGCCTGAAAGCAAGAGTCCTGAGCAAGTTTCGGCAACTGTTAAAGCATTTATGACTGCAGACCTTCCACACGAGTTGATTGAGCTTCTTGAAAAGATAGTGCTGCAAAATTCTGCTTTTAGTGGGAACTTCAATCTGCAGAATCTGTTGATTCTGACTGCCATCAAAGCTGATCCATCCAGAGTCATGGACTATATCAATAGATTAGACAACTTTGATGGACCCGCAGTTGGAGTAATGGCTGTAGAAGCTCAATTGTATGAAGAAGCATTTGCAATCTTCAAGAAGTTCAATTTGAATGTTGATGCTGTGAATGTCCTGTTGGATAACATTCAGAGCATTGACCGAGCTGTAGAGTTTGCATTCCGGGTTGAAGAAGATGCTGTTTGGAGCCAGGTGGCCAAGGCTCAATTGCGAGAGGGATTAGTGAGTGATGCCATCGAATCATTCATCCGTGCAGATGATGCCACCCACTTTTTGGATGTCATACGTGCTTCTGAGGATGCAGATGTATATCATGACTTGGTAAGGTACCTTCTGATGGTTAGGCAGAAGACCAAGGAGCCGAAGGTCGACAGTGAACTTATTTATGCATATGCTAAGATTGATCGGCTTGGTGATATTGAAGAATTCATTCTTATGCCAAATGTTGCCAATCTCCAAAATGTTGGGGATCGCTTGTTTGATGAGGCCCTATACGAAGCAGCCAAGATCATATTTGCATTCATTTCTAACTGGGGTAAACTAGCATGCACTCTTGTGAAGCTAAATCAGTTTCAAGGTGCCGTTGATGCAGCTCGGAAAGCTAACAGCTCAAAAACTTGGAAGTTTGTTTGCTTTGCATGCGTTGATGCTGAGGAATTCCGCTTAGCTCAGATTTGTGGACTTAACATCATCATTCAGGTGGATGATTTGGAAGAGGTCTGTGAGTATTACCAGAACAGGGGATGCTTTAACGAGCTCATTAGTCTCATGGAGAGCGGGCTAGGGTTGGAGCGTGCACATATGGGCATCTTTACGGAATTAGGAGTTTTATATGCAAGATACCGTCCTGAGAAGCTTATGGAACACATTAAACTGTTCTCTACCCGTCTCAACATTCCCAAGCTTATACGAGCTTGCGATGAACAGCAGCACTGGAAAGAATTAACATACTTATATATCCAATATGATGAGTTTGATAATGCTGCAACTACCATAATGAACCACTCCCCAGAAGCATGGGACCACATGCAATTCAAGGATGTTATTGTCAAAGTTGCAAGTGTTGAACTCTACTATAGGGCTGTGCACTTCTACTTAGAAGAACATCCTGAAGTTATTAATGATGTTCTTAATGTGATTGCACTTCGTGTCGACCACACCAGAGTGGTGGATATAATGAGAAAGGCTGGTCACATCCGCCTCATAAAGCCATACATGATTGCAGTTCAGAGCAACAATGTAACTGCTGTAAATGAAGCTCTAAATGAAATATACATAGAGGAGGAAGACTATGACAGGCTTCGTGAATCAATAGATTTGCACGACAGCTTTGATCAGATCGGCCTTGCTCAAAAGCTTGAGAAACATGAGCTTCTGGAGATGAGACGTGTCGCTGCATACATTTACAAGAAAGCGGGTAGATGGAAGCAATCCATTGCATTGTCAAAGAAAGACAATCATTACAAAGATTGCATGGAGACATGCTCGCAGTCTGGTGATCATGAACTTTCGGAGGAACTGCTTATCTATTTCATTGAGCAGGGAAAGAAAGAGTGTTTTGCTGCATGCCTATTTGTTTGTTATGATATGATCCGGCCAGATGTTGCTCTCGAGCTTGCGTGGATGAACAATATAATTGATTTTGCCTTACCATACCTCCTGCAGTTTATTCGAGAGTACACAGGAAAGGTTGATGAACTTATTAAAGACAAAATCGAAGCTCAAAACGAGGTGAAAGCCAAAGCGAAAGAGGAGAAGGAATTGGTTGCTCAGCAGAATATGTATGCTCAGTTGCTGCCTGCCTTGCCTGCACCACCGGGAATGGGAGGTTATGCTCCACCACCAATGCCGCCAATGGGAGGGATGGGGATGCCGCCAATGCCACCATTTGGAATGCCGCAAATGGGCCCCTCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1767

Amino Acids

199.81

Weight (kDa)

5.35

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 86 - 122 1.5e-06 Clathrin propeller repeat
Clathrin-link PF09268 410 - 433 7.8e-09 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 435 - 500 1.9e-27 Clathrin-H-link
Clathrin PF00637 623 - 758 1.4e-24 Region in Clathrin and VPS
Clathrin PF00637 767 - 908 3.2e-26 Region in Clathrin and VPS
Clathrin PF00637 917 - 1051 4.3e-32 Region in Clathrin and VPS
Clathrin PF00637 1060 - 1198 3.1e-32 Region in Clathrin and VPS
Clathrin PF00637 1211 - 1348 1.8e-28 Region in Clathrin and VPS
Clathrin PF00637 1357 - 1498 2.9e-27 Region in Clathrin and VPS
Clathrin PF00637 1505 - 1646 7e-31 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1328
Acc36I ACCTGC 2 cut(s) 526, 1747
Acc65I GGTACC 1 cut(s) 3708
AccB1I GGYRCC 4 cut(s) 194, 941, 3708, 3959
AccB7I CCANNNNNTGG 2 cut(s) 3655, 5270
AccBSI CCGCTC 2 cut(s) 646, 4158
AccI GTMKAC 4 cut(s) 32, 388, 3750, 4533
AccII CGCG 1 cut(s) 2952
AcoI YGGCCR 4 cut(s) 201, 2040, 3576, 4976
AcsI RAATTY 8 cut(s) 432, 1436, 1727, 2497, 3052, 3286, 3806, 4037
AcuI CTGAAG 7 cut(s) 1179, 1886, 1964, 1970, 2261, 2739, 4512
AdeI CACNNNGTG 1 cut(s) 4529
AfaI GTAC 9 cut(s) 311, 1242, 1417, 2289, 2375, 2435, 2862, 3710, 5060
AflIII ACRYGT 3 cut(s) 2152, 2863, 4768
AgeI ACCGGT 1 cut(s) 2764
AjiI CACGTC 2 cut(s) 2558, 4769
AjuI GAANNNNNNNTTGG 4 cut(s) 2036, 2068, 3482, 3514
AleI CACNNNNGTG 2 cut(s) 3935, 4544
Alw21I GWGCWC 5 cut(s) 2402, 3033, 4140, 4180, 4470
Alw26I GTCTC 8 cut(s) 21, 320, 1094, 1851, 4151, 4274, 4759, 4853
Alw44I GTGCAC 2 cut(s) 4176, 4466
AlwNI CAGNNNCTG 4 cut(s) 1109, 2701, 3322, 5176
Ama87I CYCGRG 2 cut(s) 1683, 4991
ApaI GGGCCC 1 cut(s) 5291
ApaLI GTGCAC 2 cut(s) 4176, 4466
ApoI RAATTY 8 cut(s) 432, 1436, 1727, 2497, 3052, 3286, 3806, 4037
ArsI GACNNNNNNTTYG 4 cut(s) 640, 672, 1975, 2007
AseI ATTAAT 3 cut(s) 2568, 3036, 4500
AsiGI ACCGGT 1 cut(s) 2764
Asp700I GAANNNNTTC 3 cut(s) 1132, 2212, 3810
Asp718I GGTACC 1 cut(s) 3708
AspA2I CCTAGG 1 cut(s) 2383
AspLEI GCGC 1 cut(s) 2390
AsuC2I CCSGG 2 cut(s) 3545, 5199
AsuHPI GGTGA 7 cut(s) 324, 629, 1879, 2596, 3806, 4889, 5125
AsuNHI GCTAGC 1 cut(s) 1591
AvaI CYCGRG 2 cut(s) 1683, 4991
AvaII GGWCC 6 cut(s) 168, 472, 1321, 3118, 3398, 4402
AvrII CCTAGG 1 cut(s) 2383
AxyI CCTNAGG 1 cut(s) 2184
BaeGI GKGCMC 5 cut(s) 946, 2119, 4180, 4470, 5291
BaeI ACNNNNGTAYC 2 cut(s) 1224, 1257
BalI TGGCCA 2 cut(s) 2042, 3578
BamHI GGATCC 1 cut(s) 2980
BanI GGYRCC 4 cut(s) 194, 941, 3708, 3959
BanII GRGCYC 6 cut(s) 2230, 2402, 2775, 3033, 4140, 5291
BarI GAAGNNNNNNTAC 2 cut(s) 3696, 3728
BauI CACGAG 1 cut(s) 3248
BbsI GAAGAC 4 cut(s) 1646, 2798, 3737, 4674
Bbv12I GWGCWC 5 cut(s) 2402, 3033, 4140, 4180, 4470
BbvCI CCTCAGC 2 cut(s) 1317, 4032
BccI CCATC 9 cut(s) 967, 1892, 3347, 3361, 3389, 3623, 3712, 4797, 5239
BceAI ACGGC 2 cut(s) 364, 3947
BcgI CGANNNNNNTGC 2 cut(s) 5033, 5067
BclI TGATCA 3 cut(s) 3157, 4714, 4879
BcnI CCSGG 2 cut(s) 3545, 5199
BcoDI GTCTC 8 cut(s) 21, 320, 1094, 1851, 4151, 4274, 4759, 4853
BfaI CTAG 8 cut(s) 249, 572, 1097, 1592, 1607, 2384, 3923, 4163
BfuAI ACCTGC 2 cut(s) 526, 1747
BglI GCCNNNNNGGC 1 cut(s) 407
BglII AGATCT 3 cut(s) 1630, 2245, 2599
BlnI CCTAGG 1 cut(s) 2383
BlpI GCTNAGC 2 cut(s) 4045, 5151
Bme18I GGWCC 6 cut(s) 168, 472, 1321, 3118, 3398, 4402
BmeT110I CYCGRG 2 cut(s) 1683, 4991
BmgBI CACGTC 2 cut(s) 2558, 4769
BmrI ACTGGG 1 cut(s) 3922
BmtI GCTAGC 1 cut(s) 1595
BmuI ACTGGG 1 cut(s) 3922
BpiI GAAGAC 4 cut(s) 1646, 2798, 3737, 4674
BplI GAGNNNNNCTC 2 cut(s) 4850, 4882
BpmI CTGGAG 4 cut(s) 981, 2386, 3053, 4778
Bpu10I CCTNAGC 7 cut(s) 531, 1317, 1965, 2913, 3114, 3198, 4032
Bpu1102I GCTNAGC 2 cut(s) 4045, 5151
BpuEI CTTGAG 3 cut(s) 435, 2252, 4760
BpuMI CCSGG 2 cut(s) 3545, 5199
Bsa29I ATCGAT 3 cut(s) 357, 2068, 2413
BsaAI YACGTR 1 cut(s) 3668
BsaBI GATNNNNATC 1 cut(s) 3690
BsaJI CCNNGG 9 cut(s) 190, 198, 457, 848, 1815, 1816, 2383, 3579, 3735
BsaWI WCCGGW 1 cut(s) 2764
BsaXI ACNNNNNCTCC 5 cut(s) 34, 1620, 1650, 5022, 5052
Bse118I RCCGGY 1 cut(s) 2764
Bse1I ACTGG 5 cut(s) 1019, 2461, 2706, 3917, 4319
Bse21I CCTNAGG 1 cut(s) 2184
Bse3DI GCAATG 2 cut(s) 3023, 4815
Bse8I GATNNNNATC 1 cut(s) 3690
BseCI ATCGAT 3 cut(s) 357, 2068, 2413
BseDI CCNNGG 9 cut(s) 190, 198, 457, 848, 1815, 1816, 2383, 3579, 3735
BseJI GATNNNNATC 1 cut(s) 3690
BseMI GCAATG 2 cut(s) 3023, 4815
BseNI ACTGG 5 cut(s) 1019, 2461, 2706, 3917, 4319
BseRI GAGGAG 4 cut(s) 9, 12, 4676, 5147
BseSI GKGCMC 5 cut(s) 946, 2119, 4180, 4470, 5291
BseYI CCCAGC 1 cut(s) 945
BsgI GTGCAG 2 cut(s) 3654, 5175
Bsh1236I CGCG 1 cut(s) 2952
BshNI GGYRCC 4 cut(s) 194, 941, 3708, 3959
BshTI ACCGGT 1 cut(s) 2764
BshVI ATCGAT 3 cut(s) 357, 2068, 2413
BsiHKAI GWGCWC 5 cut(s) 2402, 3033, 4140, 4180, 4470
BsiHKCI CYCGRG 2 cut(s) 1683, 4991
BsiSI CCGG 4 cut(s) 2765, 3544, 4975, 5198
BslFI GGGAC 1 cut(s) 4415
BsmAI GTCTC 8 cut(s) 21, 320, 1094, 1851, 4151, 4274, 4759, 4853
BsmBI CGTCTC 3 cut(s) 21, 4274, 4759
BsmFI GGGAC 1 cut(s) 4415
BsmI GAATGC 3 cut(s) 3539, 3899, 5280
BsoBI CYCGRG 2 cut(s) 1683, 4991
Bsp120I GGGCCC 1 cut(s) 5287
Bsp1407I TGTACA 2 cut(s) 2287, 2860
Bsp1720I GCTNAGC 2 cut(s) 4045, 5151
Bsp19I CCATGG 1 cut(s) 198
BspDI ATCGAT 3 cut(s) 357, 2068, 2413
BspFNI CGCG 1 cut(s) 2952
BspHI TCATGA 2 cut(s) 3694, 4882
BspMAI CTGCAG 5 cut(s) 1114, 1381, 3238, 3318, 5046
BspMI ACCTGC 2 cut(s) 526, 1747
BspOI GCTAGC 1 cut(s) 1595
BspQI GCTCTTC 1 cut(s) 1614
BspT107I GGYRCC 4 cut(s) 194, 941, 3708, 3959
BsrBI CCGCTC 2 cut(s) 646, 4158
BsrDI GCAATG 2 cut(s) 3023, 4815
BsrFI RCCGGY 1 cut(s) 2764
BsrGI TGTACA 2 cut(s) 2287, 2860
BsrI ACTGG 5 cut(s) 1019, 2461, 2706, 3917, 4319
BssAI RCCGGY 1 cut(s) 2764
BssECI CCNNGG 9 cut(s) 190, 198, 457, 848, 1815, 1816, 2383, 3579, 3735
BssNAI GTATAC 1 cut(s) 33
BssSI CACGAG 1 cut(s) 3248
BssT1I CCWWGG 5 cut(s) 198, 457, 2383, 3579, 3735
Bst1107I GTATAC 1 cut(s) 33
Bst2BI CACGAG 1 cut(s) 3248
Bst6I CTCTTC 4 cut(s) 30, 1614, 2763, 4089
BstAPI GCANNNNNTGC 6 cut(s) 382, 821, 830, 1385, 1712, 3641
BstAUI TGTACA 2 cut(s) 2287, 2860
BstBAI YACGTR 1 cut(s) 3668
BstDSI CCRYGG 1 cut(s) 198
BstENI CCTNNNNNAGG 1 cut(s) 2780
BstFNI CGCG 1 cut(s) 2952
BstHHI GCGC 1 cut(s) 2390
BstMAI GTCTC 8 cut(s) 21, 320, 1094, 1851, 4151, 4274, 4759, 4853
BstSLI GKGCMC 5 cut(s) 946, 2119, 4180, 4470, 5291
BstUI CGCG 1 cut(s) 2952
BstV2I GAAGAC 4 cut(s) 1646, 2798, 3737, 4674
BstX2I RGATCY 8 cut(s) 51, 1138, 1630, 1810, 2245, 2599, 2980, 3167
BstXI CCANNNNNNTGG 4 cut(s) 3364, 3845, 4398, 4549
BstYI RGATCY 8 cut(s) 51, 1138, 1630, 1810, 2245, 2599, 2980, 3167
BstZ17I GTATAC 1 cut(s) 33
Bsu15I ATCGAT 3 cut(s) 357, 2068, 2413
Bsu36I CCTNAGG 1 cut(s) 2184
BsuTUI ATCGAT 3 cut(s) 357, 2068, 2413
BtgI CCRYGG 1 cut(s) 198
BtgZI GCGATG 1 cut(s) 4314
BtrI CACGTC 2 cut(s) 2558, 4769
BtsI GCAGTG 1 cut(s) 1287
BtsIMutI CAGTG 5 cut(s) 1287, 1650, 2130, 3760, 4312
BveI ACCTGC 2 cut(s) 526, 1747
CaiI CAGNNNCTG 4 cut(s) 1109, 2701, 3322, 5176
CciI TCATGA 2 cut(s) 3694, 4882
CfoI GCGC 1 cut(s) 2390
Cfr10I RCCGGY 1 cut(s) 2764
ClaI ATCGAT 3 cut(s) 357, 2068, 2413
CseI GACGC 2 cut(s) 334, 374
CsiI ACCWGGT 1 cut(s) 1323
Csp6I GTAC 9 cut(s) 310, 1241, 1416, 2288, 2374, 2434, 2861, 3709, 5059
CspAI ACCGGT 1 cut(s) 2764
CspCI CAANNNNNGTGG 4 cut(s) 1461, 1496, 3234, 3269
CviQI GTAC 9 cut(s) 310, 1241, 1416, 2288, 2374, 2434, 2861, 3709, 5059
DraI TTTAAA 1 cut(s) 841
DraIII CACNNNGTG 1 cut(s) 4529
DrdI GACNNNNNNGTC 1 cut(s) 1328
DseDI GACNNNNNNGTC 1 cut(s) 1328
EaeI YGGCCR 4 cut(s) 201, 2040, 3576, 4976
Eam1104I CTCTTC 4 cut(s) 30, 1614, 2763, 4089
EarI CTCTTC 4 cut(s) 30, 1614, 2763, 4089
EciI GGCGGA 1 cut(s) 4568
Ecl136II GAGCTC 3 cut(s) 2400, 3031, 4138
Eco130I CCWWGG 5 cut(s) 198, 457, 2383, 3579, 3735
Eco24I GRGCYC 6 cut(s) 2230, 2402, 2775, 3033, 4140, 5291
Eco32I GATATC 2 cut(s) 1035, 2146
Eco47I GGWCC 6 cut(s) 168, 472, 1321, 3118, 3398, 4402
Eco53kI GAGCTC 3 cut(s) 2400, 3031, 4138
Eco57I CTGAAG 7 cut(s) 1179, 1886, 1964, 1970, 2261, 2739, 4512
Eco81I CCTNAGG 1 cut(s) 2184
Eco88I CYCGRG 2 cut(s) 1683, 4991
EcoICRI GAGCTC 3 cut(s) 2400, 3031, 4138
EcoNI CCTNNNNNAGG 1 cut(s) 2780
EcoO109I RGGNCCY 6 cut(s) 168, 235, 1321, 3118, 3868, 5288
EcoRI GAATTC 3 cut(s) 432, 3806, 4037
EcoRV GATATC 2 cut(s) 1035, 2146
EcoT14I CCWWGG 5 cut(s) 198, 457, 2383, 3579, 3735
EcoT22I ATGCAT 2 cut(s) 927, 3772
EcoT38I GRGCYC 6 cut(s) 2230, 2402, 2775, 3033, 4140, 5291
ErhI CCWWGG 5 cut(s) 198, 457, 2383, 3579, 3735
Esp3I CGTCTC 3 cut(s) 21, 4274, 4759
FalI AAGNNNNNCTT 2 cut(s) 2529, 2561
FaqI GGGAC 1 cut(s) 4415
FauI CCCGC 5 cut(s) 1453, 1797, 3409, 4151, 4789
FauNDI CATATG 2 cut(s) 3772, 4182
FbaI TGATCA 3 cut(s) 3157, 4714, 4879
FblI GTMKAC 4 cut(s) 32, 388, 3750, 4533
FriOI GRGCYC 6 cut(s) 2230, 2402, 2775, 3033, 4140, 5291
FspBI CTAG 8 cut(s) 249, 572, 1097, 1592, 1607, 2384, 3923, 4163
GlaI GCGC 1 cut(s) 2389
GsaI CCCAGC 1 cut(s) 949
GsuI CTGGAG 4 cut(s) 981, 2386, 3053, 4778
HapII CCGG 4 cut(s) 2765, 3544, 4975, 5198
HgaI GACGC 2 cut(s) 334, 374
HhaI GCGC 1 cut(s) 2390
Hin6I GCGC 1 cut(s) 2388
HinP1I GCGC 1 cut(s) 2388
HincII GTYRAC 4 cut(s) 389, 2084, 3751, 4534
HindII GTYRAC 4 cut(s) 389, 2084, 3751, 4534
HindIII AAGCTT 7 cut(s) 132, 1070, 1161, 2543, 4235, 4283, 4736
HpaII CCGG 4 cut(s) 2765, 3544, 4975, 5198
HphI GGTGA 7 cut(s) 324, 629, 1879, 2596, 3806, 4889, 5125
Hpy99I CGWCG 2 cut(s) 350, 390
HpyCH4IV ACGT 8 cut(s) 14, 242, 278, 1546, 2152, 2557, 3667, 4768
HpySE526I ACGT 8 cut(s) 14, 242, 278, 1546, 2152, 2557, 3667, 4768
HspAI GCGC 1 cut(s) 2388
KpnI GGTACC 1 cut(s) 3712
Ksp22I TGATCA 3 cut(s) 3157, 4714, 4879
LguI GCTCTTC 1 cut(s) 1614
LmnI GCTCC 9 cut(s) 733, 2405, 2476, 2683, 2830, 3567, 3739, 4171, 5221
MabI ACCWGGT 1 cut(s) 1323
MaeI CTAG 8 cut(s) 249, 572, 1097, 1592, 1607, 2384, 3923, 4163
MaeII ACGT 8 cut(s) 14, 242, 278, 1546, 2152, 2557, 3667, 4768
MbiI CCGCTC 2 cut(s) 646, 4158
MfeI CAATTG 4 cut(s) 1236, 2175, 3431, 3587
MflI RGATCY 8 cut(s) 51, 1138, 1630, 1810, 2245, 2599, 2980, 3167
MlsI TGGCCA 2 cut(s) 2042, 3578
MluNI TGGCCA 2 cut(s) 2042, 3578
MlyI GAGTC 4 cut(s) 398, 1294, 3202, 3369
MmeI TCCRAC 6 cut(s) 1741, 1920, 3145, 3388, 3480, 4149
Mox20I TGGCCA 2 cut(s) 2042, 3578
Mph1103I ATGCAT 2 cut(s) 927, 3772
MroXI GAANNNNTTC 3 cut(s) 1132, 2212, 3810
MscI TGGCCA 2 cut(s) 2042, 3578
MslI CAYNNNNRTG 8 cut(s) 224, 359, 371, 497, 3815, 3935, 4079, 4544
Msp20I TGGCCA 2 cut(s) 2042, 3578
MspA1I CMGCKG 3 cut(s) 1157, 1336, 2447
MspI CCGG 4 cut(s) 2765, 3544, 4975, 5198
MunI CAATTG 4 cut(s) 1236, 2175, 3431, 3587
Mva1269I GAATGC 3 cut(s) 3539, 3899, 5280
MvnI CGCG 1 cut(s) 2952
NciI CCSGG 2 cut(s) 3545, 5199
NcoI CCATGG 1 cut(s) 198
NdeI CATATG 2 cut(s) 3772, 4182
NheI GCTAGC 1 cut(s) 1591
NmeAIII GCCGAG 1 cut(s) 386
NmuCI GTSAC 7 cut(s) 289, 580, 1542, 2008, 2482, 3020, 4571
NsiI ATGCAT 2 cut(s) 927, 3772
OliI CACNNNNGTG 2 cut(s) 3935, 4544
PaeI GCATGC 3 cut(s) 3930, 4023, 4950
PaeR7I CTCGAG 2 cut(s) 1683, 4991
PagI TCATGA 2 cut(s) 3694, 4882
PasI CCCWGGG 1 cut(s) 1816
PciI ACATGT 1 cut(s) 2863
PciSI GCTCTTC 1 cut(s) 1614
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 3 cut(s) 3539, 3899, 5280
PdmI GAANNNNTTC 3 cut(s) 1132, 2212, 3810
PflMI CCANNNNNTGG 2 cut(s) 3655, 5270
PfoI TCCNGGA 1 cut(s) 2668
PinAI ACCGGT 1 cut(s) 2764
PleI GAGTC 4 cut(s) 398, 1293, 3201, 3368
PpsI GAGTC 4 cut(s) 398, 1293, 3201, 3368
Ppu21I YACGTR 1 cut(s) 3668
PpuMI RGGWCCY 3 cut(s) 168, 1321, 3118
PscI ACATGT 1 cut(s) 2863
PshBI ATTAAT 3 cut(s) 2568, 3036, 4500
Psp124BI GAGCTC 3 cut(s) 2402, 3033, 4140
Psp5II RGGWCCY 3 cut(s) 168, 1321, 3118
PspFI CCCAGC 1 cut(s) 945
PspOMI GGGCCC 1 cut(s) 5287
PspPPI RGGWCCY 3 cut(s) 168, 1321, 3118
PspXI VCTCGAGB 1 cut(s) 1683
PstI CTGCAG 5 cut(s) 1114, 1381, 3238, 3318, 5046
PstNI CAGNNNCTG 4 cut(s) 1109, 2701, 3322, 5176
PsuI RGATCY 8 cut(s) 51, 1138, 1630, 1810, 2245, 2599, 2980, 3167
PvuII CAGCTG 2 cut(s) 1157, 2447
RsaI GTAC 9 cut(s) 311, 1242, 1417, 2289, 2375, 2435, 2862, 3710, 5060
RsaNI GTAC 9 cut(s) 310, 1241, 1416, 2288, 2374, 2434, 2861, 3709, 5059
RseI CAYNNNNRTG 8 cut(s) 224, 359, 371, 497, 3815, 3935, 4079, 4544
SacI GAGCTC 3 cut(s) 2402, 3033, 4140
SalI GTCGAC 3 cut(s) 387, 3749, 4532
SapI GCTCTTC 1 cut(s) 1614
SchI GAGTC 4 cut(s) 398, 1294, 3202, 3369
SexAI ACCWGGT 1 cut(s) 1323
Sfr274I CTCGAG 2 cut(s) 1683, 4991
SinI GGWCC 6 cut(s) 168, 472, 1321, 3118, 3398, 4402
SlaI CTCGAG 2 cut(s) 1683, 4991
SmiMI CAYNNNNRTG 8 cut(s) 224, 359, 371, 497, 3815, 3935, 4079, 4544
SmlI CTYRAG 5 cut(s) 450, 1683, 2231, 4739, 4991
SmoI CTYRAG 5 cut(s) 450, 1683, 2231, 4739, 4991
SphI GCATGC 3 cut(s) 3930, 4023, 4950
SspI AATATT 2 cut(s) 486, 2966
SspMI CTAG 8 cut(s) 249, 572, 1097, 1592, 1607, 2384, 3923, 4163
SstI GAGCTC 3 cut(s) 2402, 3033, 4140
StyI CCWWGG 5 cut(s) 198, 457, 2383, 3579, 3735
TaiI ACGT 8 cut(s) 17, 245, 281, 1549, 2155, 2560, 3670, 4771
TaqII GACCGA 1 cut(s) 3537
TatI WGTACW 6 cut(s) 309, 2287, 2373, 2433, 2860, 5058
TauI GCSGC 6 cut(s) 206, 209, 1391, 5233, 5257, 5281
TscAI CASTG 5 cut(s) 1287, 1650, 2137, 3760, 4319
TseFI GTSAC 7 cut(s) 289, 580, 1542, 2008, 2482, 3020, 4571
Tsp45I GTSAC 7 cut(s) 289, 580, 1542, 2008, 2482, 3020, 4571
TspGWI ACGGA 4 cut(s) 1403, 1907, 3620, 4211
TspRI CASTG 5 cut(s) 1287, 1650, 2137, 3760, 4319
Van91I CCANNNNNTGG 2 cut(s) 3655, 5270
VneI GTGCAC 2 cut(s) 4176, 4466
VpaK11BI GGWCC 6 cut(s) 168, 472, 1321, 3118, 3398, 4402
VspI ATTAAT 3 cut(s) 2568, 3036, 4500
XagI CCTNNNNNAGG 1 cut(s) 2780
XapI RAATTY 8 cut(s) 432, 1436, 1727, 2497, 3052, 3286, 3806, 4037
XcmI CCANNNNNNNNNTGG 2 cut(s) 3082, 3652
XhoI CTCGAG 2 cut(s) 1683, 4991
XmaJI CCTAGG 1 cut(s) 2383
XmiI GTMKAC 4 cut(s) 32, 388, 3750, 4533
XmnI GAANNNNTTC 3 cut(s) 1132, 2212, 3810
XspI CTAG 8 cut(s) 249, 572, 1097, 1592, 1607, 2384, 3923, 4163
Zsp2I ATGCAT 2 cut(s) 927, 3772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.