pycom03g15020

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002678
Physical Location & Seq
Reverse (-)
16391668 .. 16392445
778 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGGTGTCGAATCTAAAGGAACAATTACAGAGAATACATGCTCTAGGCGCGCAAAAATTTGTAGTCATCGGGACCGGGCCGGTTGGATGCTGTCCGTCGCAAAGGAATTCAAGTACAGGAGAGTGCAATGATGGTGCAAACTCATTGTCTGTCATGTACAAGGAAGCCCTTAAGTCCATGTTGCAGCAATTGAAACCCGCGTTGGGCATAAGCTACACCCTCTTTGACACTTACACTATCTTGAATAACATCATTCAAAACCCCGCTTCTTACGGATTTACAGAGGTTAAAGCTGCATGCTGTGGGATTGGGAAGCGTAATGCCAAGGGTCCATGCACACCAATTTCATCTTTGTGCTCCAACAGGAGGGACCATGTCTTCTGGGATTTTTACCATCCTACACAGGCAACTCATGGCATCATAACGGATAAACTTTTCGATGGCCCTTCAGAATACTCATCCCCAATGACTGTGAAGGAGTTAATTGCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

165

Amino Acids

17.88

Weight (kDa)

8.47

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 2 - 144 2.5e-14 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 50, 200
AciI CCGC 2 cut(s) 198, 264
AcsI RAATTY 2 cut(s) 56, 106
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 2 cut(s) 115, 158
AfiI CCNNNNNNNGG 2 cut(s) 203, 366
AflII CTTAAG 1 cut(s) 170
AgsI TTSAA 4 cut(s) 111, 193, 244, 257
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AluBI AGCT 2 cut(s) 213, 293
AluI AGCT 2 cut(s) 213, 293
Alw21I GWGCWC 1 cut(s) 359
AoxI GGCC 2 cut(s) 77, 442
ApeKI GCWGC 2 cut(s) 184, 293
ApoI RAATTY 2 cut(s) 56, 106
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspLEI GCGC 2 cut(s) 50, 52
AspS9I GGNCC 5 cut(s) 72, 77, 329, 370, 443
AsuC2I CCSGG 1 cut(s) 76
AvaII GGWCC 3 cut(s) 72, 329, 370
BbsI GAAGAC 1 cut(s) 370
Bbv12I GWGCWC 1 cut(s) 359
BbvI GCAGC 2 cut(s) 196, 280
BccI CCATC 3 cut(s) 125, 402, 434
BcnI CCSGG 1 cut(s) 76
BfaI CTAG 1 cut(s) 44
BfrI CTTAAG 1 cut(s) 170
BisI GCNGC 2 cut(s) 185, 294
BlsI GCNGC 2 cut(s) 186, 295
Bme1390I CCNGG 1 cut(s) 76
Bme18I GGWCC 3 cut(s) 72, 329, 370
BmgT120I GGNCC 5 cut(s) 72, 77, 329, 370, 443
BmiI GGNNCC 3 cut(s) 73, 330, 371
BmrFI CCNGG 1 cut(s) 76
BmsI GCATC 2 cut(s) 77, 426
BpiI GAAGAC 1 cut(s) 370
BpuMI CCSGG 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 324
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 366
Bse118I RCCGGY 1 cut(s) 79
Bse3DI GCAATG 1 cut(s) 133
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 3 cut(s) 92, 394, 458
BseLI CCNNNNNNNGG 2 cut(s) 203, 366
BseMI GCAATG 1 cut(s) 133
BsePI GCGCGC 1 cut(s) 48
BseXI GCAGC 2 cut(s) 196, 280
Bsh1236I CGCG 2 cut(s) 50, 200
BshFI GGCC 2 cut(s) 79, 444
BsiHKAI GWGCWC 1 cut(s) 359
BsiSI CCGG 2 cut(s) 75, 80
BslFI GGGAC 2 cut(s) 85, 383
BslI CCNNNNNNNGG 2 cut(s) 203, 366
BsmFI GGGAC 2 cut(s) 85, 383
BsnI GGCC 2 cut(s) 79, 444
Bsp1286I GDGCHC 1 cut(s) 359
Bsp1407I TGTACA 1 cut(s) 156
BspACI CCGC 2 cut(s) 198, 264
BspANI GGCC 2 cut(s) 79, 444
BspFNI CGCG 2 cut(s) 50, 200
BspLI GGNNCC 3 cut(s) 73, 330, 371
BspTI CTTAAG 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 133
BsrFI RCCGGY 1 cut(s) 79
BsrGI TGTACA 1 cut(s) 156
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 324
BssHII GCGCGC 1 cut(s) 48
BssT1I CCWWGG 1 cut(s) 324
Bst4CI ACNGT 1 cut(s) 472
BstAFI CTTAAG 1 cut(s) 170
BstAUI TGTACA 1 cut(s) 156
BstC8I GCNNGC 2 cut(s) 50, 298
BstF5I GGATG 3 cut(s) 92, 394, 458
BstFNI CGCG 2 cut(s) 50, 200
BstHHI GCGC 2 cut(s) 50, 52
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNSI RCATGY 2 cut(s) 41, 300
BstSCI CCNGG 1 cut(s) 74
BstUI CGCG 2 cut(s) 50, 200
BstV1I GCAGC 2 cut(s) 196, 280
BstV2I GAAGAC 1 cut(s) 370
BsuRI GGCC 2 cut(s) 79, 444
BtsCI GGATG 3 cut(s) 92, 394, 458
Cac8I GCNNGC 2 cut(s) 50, 298
CfoI GCGC 2 cut(s) 50, 52
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 5 cut(s) 72, 77, 329, 370, 443
Csp6I GTAC 2 cut(s) 114, 157
CviAII CATG 7 cut(s) 38, 154, 178, 297, 333, 374, 413
CviJI RGCY 5 cut(s) 79, 167, 213, 293, 444
CviKI_1 RGCY 5 cut(s) 79, 167, 213, 293, 444
CviQI GTAC 2 cut(s) 114, 157
Eco130I CCWWGG 1 cut(s) 324
Eco47I GGWCC 3 cut(s) 72, 329, 370
Eco57I CTGAAG 1 cut(s) 432
EcoRI GAATTC 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 7 cut(s) 41, 157, 181, 300, 336, 377, 416
FaqI GGGAC 2 cut(s) 85, 383
FatI CATG 7 cut(s) 37, 153, 177, 296, 332, 373, 412
FauI CCCGC 2 cut(s) 205, 271
Fnu4HI GCNGC 2 cut(s) 185, 294
FokI GGATG 3 cut(s) 99, 381, 445
Fsp4HI GCNGC 2 cut(s) 185, 294
FspBI CTAG 1 cut(s) 44
GlaI GCGC 2 cut(s) 49, 51
GluI GCNGC 2 cut(s) 185, 294
HaeIII GGCC 2 cut(s) 79, 444
HapII CCGG 2 cut(s) 75, 80
HhaI GCGC 2 cut(s) 50, 52
Hin1II CATG 7 cut(s) 41, 157, 181, 300, 336, 377, 416
Hin6I GCGC 2 cut(s) 48, 50
HinP1I GCGC 2 cut(s) 48, 50
HinfI GANTC 1 cut(s) 10
HpaII CCGG 2 cut(s) 75, 80
Hpy188I TCNGA 1 cut(s) 451
Hpy188III TCNNGA 2 cut(s) 70, 241
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 2 cut(s) 456, 469
HpyCH4III ACNGT 1 cut(s) 472
HpyCH4V TGCA 5 cut(s) 126, 137, 184, 296, 336
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
Hsp92II CATG 7 cut(s) 41, 157, 181, 300, 336, 377, 416
HspAI GCGC 2 cut(s) 48, 50
LmnI GCTCC 1 cut(s) 362
LpnPI CCDG 6 cut(s) 88, 93, 102, 349, 367, 389
Lsp1109I GCAGC 2 cut(s) 196, 280
LweI GCATC 2 cut(s) 77, 426
MaeI CTAG 1 cut(s) 44
MboII GAAGA 1 cut(s) 370
MfeI CAATTG 1 cut(s) 188
MhlI GDGCHC 1 cut(s) 359
MluCI AATT 6 cut(s) 23, 56, 106, 188, 342, 483
MmeI TCCRAC 2 cut(s) 64, 384
MnlI CCTC 3 cut(s) 230, 277, 360
MseI TTAA 3 cut(s) 171, 288, 482
MslI CAYNNNNRTG 1 cut(s) 352
MspCI CTTAAG 1 cut(s) 170
MspI CCGG 2 cut(s) 75, 80
MspR9I CCNGG 1 cut(s) 76
MunI CAATTG 1 cut(s) 188
MvnI CGCG 2 cut(s) 50, 200
MwoI GCNNNNNNNGC 1 cut(s) 47
NciI CCSGG 1 cut(s) 76
NlaIII CATG 7 cut(s) 41, 157, 181, 300, 336, 377, 416
NlaIV GGNNCC 3 cut(s) 73, 330, 371
NspI RCATGY 2 cut(s) 41, 300
PaeI GCATGC 1 cut(s) 300
PauI GCGCGC 1 cut(s) 48
PfeI GAWTC 1 cut(s) 10
PflFI GACNNNGTC 1 cut(s) 374
PkrI GCNGC 2 cut(s) 186, 295
PspN4I GGNNCC 3 cut(s) 73, 330, 371
PspPI GGNCC 5 cut(s) 72, 77, 329, 370, 443
PsyI GACNNNGTC 1 cut(s) 374
PteI GCGCGC 1 cut(s) 48
RsaI GTAC 2 cut(s) 115, 158
RsaNI GTAC 2 cut(s) 114, 157
RseI CAYNNNNRTG 1 cut(s) 352
SaqAI TTAA 3 cut(s) 171, 288, 482
SatI GCNGC 2 cut(s) 185, 294
Sau96I GGNCC 5 cut(s) 72, 77, 329, 370, 443
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 1 cut(s) 359
SetI ASST 3 cut(s) 215, 288, 295
SfaNI GCATC 2 cut(s) 77, 426
SinI GGWCC 3 cut(s) 72, 329, 370
SmiMI CAYNNNNRTG 1 cut(s) 352
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
SphI GCATGC 1 cut(s) 300
Sse9I AATT 6 cut(s) 23, 56, 106, 188, 342, 483
SsiI CCGC 2 cut(s) 198, 264
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 74
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 472
TaqI TCGA 2 cut(s) 8, 438
TasI AATT 6 cut(s) 23, 56, 106, 188, 342, 483
TatI WGTACW 2 cut(s) 113, 156
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 3 cut(s) 171, 288, 482
Tru9I TTAA 3 cut(s) 171, 288, 482
TseI GCWGC 2 cut(s) 184, 293
TspDTI ATGAA 1 cut(s) 336
TspGWI ACGGA 3 cut(s) 84, 288, 440
Tth111I GACNNNGTC 1 cut(s) 374
Vha464I CTTAAG 1 cut(s) 170
VpaK11BI GGWCC 3 cut(s) 72, 329, 370
XapI RAATTY 2 cut(s) 56, 106
XceI RCATGY 2 cut(s) 41, 300
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.