pycom07g20230

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002740
Physical Location & Seq
Forward (+)
22396944 .. 22404150
7207 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3012 bp
ATGAAGGAGGCCCTAACGCTTCCGAGCGTTGGGATTAATCCACAATTCATAACGTTTACACATGTTACTATGGAATCCGATAAGTACATATGCGTTCGGGAGACTTCGCCTCAGAACAGTATTGTTATTATCGATATGAGTATGCCGATGCAACCTCTGAGGAGGCCTATCACCGCGGACTCTGCACTTATGAATCCGAATTCCAAAATTCTTGCCTTGAAAGCTGGAGTCCAAGGTACTACTCAGGATCATCTTCAAATATTTAACATCGAGATGAAAGCAAAACTGAAATCACATCTTATGCCGGAGCAGATTGTTTTCTGGAAGTGGATAACCCCAAAGATGTTGGGTTTAGTCACTCAGACTACAGTATACCACTGGTCAATTGAAGGTGAATCTGAACCTGTAAAGGTGTTTGAGCGAACAGCTAATCTGGCTAACAATCAGATTATAAATTACCGCTGTGATCCTTCCGAGAAGTGGTTGGTTTTGATTGGAATTGCTCCTGGTGCACCAGAGAGGCCACAATTGGTGAAAGGAAATTTGCAACTCTTCTCCGTGGATCAGCAGCGCAGTCAAGCTCTTGAAGCACATGCTGCATCATTTGCCCAATACAAGGTCCCAGGGAATGAGAATTCTTCAACTCTTATTTCTTTTGCCACTAAGACCCTCAATGCTGGACAGATTACATCAAAGTTGCATGTTATTGAGCTCGGTGCCCAGCCAGGGAAACCATCTTTTACGAAGAAACAAGCCGATCTTTTCTTTCCTCCAGATTTTAATGATGATTTCCCAGTCGCCATGCAGATGTCTCACAAGTACAGCTTGATTTATGTAATTACAAAGCTTGGGCTACTATTTGTGTATGATTTAGAGACAGCTAGTGCCGTTTACAGAAATAGAATTAGTCCGGATCCAATTTTCTTGACAACGGAGGCTTCATCTGTTGGAGGGTTTTATGCGGTTAATAGGCGGGGCCAGGTGTTGTTGGCTACAATTAATGAGCAAACAATTGTGCCTTTTGTCAGCGGCCAGCTAAACAATTTGGAGCTTGCTGTCAATCTGGCTAAAAGAGGAAATCTTCCAGGTGCTGAGAATCTGGTTGTCCAGCGTTTTCAAGAACTGTTTGCCCAAACGAAGTACAAAGAAGCTGCTGAGCTTGCTGCGGAGTCCCCACAAGGAATTCTCCGCACACCTGATACTGTTGCTAAATTTCAGAGTGTTCCTGTCCAAGCTGGGCAAACACCACCTTTGTTGCAGTACTTTGGGACACTCTTGACAAGAGGGAAGCTCAACGCATTTGAATCATTGGAATTGTCACGTCTTGTGGTGAACCAGAATAAGAAGAACCTTTTGGAGAACTGGTTGGCCGAGGACAAACTCGAATGCAGTGAGGAACTGGGAGATCTTGTGAAGACTGTGGATAATGATCTGGCTTTGAAAATATATATTAAAGCCAGAGCAACTCCCAAAGTTGTTGCAGCTTTTGCTGAGCGAAGGGAGTTTGACAAAATACTGATCTACTCAAAGCAGGTTGGCTACACACCTGACTACCTGTTCCTTTTGCAAACAATTCTTCGAGCAGATCCTCAGGGTGCTGTTAATTTTGCATTAATGATGTCTCAAATGGAAGGTGGTTGTCCAGTTGATTACAACACTATAACAGATCTCTTCCTTCAGAGGAACCTTATTCGTGAGGCAACCGCCTTTCTATTGGATGTTTTAAAGCCAAATCTTCCAGAGCATGCATTCCTGCAAACAAAGGTCCTTGAGATCAATTTAGTGACTTTTCCTAATGTGGCCGATGCTATTTTAGCCAATGGAATGTTCAGTCATTATGACCGTCCTCGTATTGCCCAGCTATGCGAAAAAGCTGGTCTTTATGTGCGAGCTTTGCAACATTACTCAGAGTTGCCTGATATTAAACGTGTCATAGTGAATACACATGCAATAGAGCCACAGTCACTTGTGGAGTTTTTTGGCACTCTGTCGAAAGAATGGGCGTTGGAGTGCATGAAAGATCTTTTATTGGTCAACTTAAGAGGAAACCTTCAGATCATTGTGCAGGTTGCTAAGGAATATTCTGAGCAGTTGGGTGTTGATCAATGCATGAAGCTCTTTGAACAGTTCAAGTCATATGAAGGGTTGTACTTCTTCCTTGGTTCGTTTTTGAGCTCAAGCAATTTGTTTAGGGCTGATATCCACTTCAAGTACATTGAGGCAGCTGCTAAAACTGGACAAATCAAGGAGGTGGAGCGAGTTACAAGAGAATCAAACTTCTATGACGCTGAGAAGACAAAGAACTTCTTAATGGAAGCTAAGCTCCCCGATGCACGTCCATTAATTAATGTTTGTGATCGTTTTGGATTTGTTCCAGATCTCACCCACTACTTGTACACGAGCAATATGCTTCGTTACATTGAAGGTTACGTTCAGAAGGTGAACCCTGGGAATGCTCCATTAGTTGTGGGACAGTTGCTAGATGATGAGTGCCCTGAAGATTTTATCAAAGGCTTGATTCTTTCTGTTCGTTCGTTGCTTCCAGTGGAGCCCCTTGTGGAGGAATGTGAGAAGAGGAATCGCCTTCGTTTACTCACACAGTTCTTGGAGCATCTTGTGAGTGAGGGAAGCCAAGATGTACATGTGCACAATGCTTTGGGTAAAATCATCATTGATAGTGGCAATAATCCAGAGCACTTCCTTACCACAAACCCGTATTATGATTCACGTGTTGTTGGTAAATATTGTGAGAAACGTGATCCTACCCTAGCTGTTGTTGCTTACCGCCGTGGACAATGTGACGATGAACTTATTAATGTCACAAATAAGAACTCATTGTTCAAATTGCAAGCAAGGTATGTGGTTGAGAGGATGGATGAAGATCTTCCTGGGGGAAAGTTCTTGACCCTGAGAATGAGTACAGAAGACAGCTTATTGATCAAGTTGTTTCTACTGCCTTGCCCGAGAGCAAGAGCCCAGAACAAGTTTCTGCGGCTGTTAAGGCTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1004

Amino Acids

113.31

Weight (kDa)

6.54

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 11 - 46 2.6e-06 Clathrin propeller repeat
Clathrin-link PF09268 335 - 358 5.2e-09 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 360 - 425 2.1e-28 Clathrin-H-link
Clathrin PF00637 548 - 683 3.9e-25 Region in Clathrin and VPS
Clathrin PF00637 692 - 834 1.3e-24 Region in Clathrin and VPS
Clathrin PF00637 843 - 962 6.8e-29 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 452
Acc36I ACCTGC 2 cut(s) 1522, 2056
AccB1I GGYRCC 1 cut(s) 716
AccI GTMKAC 1 cut(s) 372
AccII CGCG 1 cut(s) 176
AccIII TCCGGA 1 cut(s) 910
AclI AACGTT 1 cut(s) 53
AclWI GGATC 7 cut(s) 255, 461, 570, 908, 921, 1580, 2753
AcoI YGGCCR 3 cut(s) 1030, 1368, 1800
AcsI RAATTY 6 cut(s) 199, 207, 541, 634, 1182, 1211
AcuI CTGAAG 3 cut(s) 1661, 2036, 2517
AcvI CACGTG 1 cut(s) 2729
AfiI CCNNNNNNNGG 5 cut(s) 29, 480, 616, 726, 2560
AflII CTTAAG 1 cut(s) 2038
AflIII ACRYGT 4 cut(s) 61, 1927, 2641, 2728
AjiI CACGTC 2 cut(s) 1322, 2336
AjnI CCWGG 7 cut(s) 505, 622, 724, 978, 1084, 2446, 2887
AjuI GAANNNNNNNTTGG 4 cut(s) 1337, 1369, 1811, 1843
Alw21I GWGCWC 5 cut(s) 514, 714, 2177, 2649, 2697
Alw26I GTCTC 4 cut(s) 95, 816, 869, 1626
Alw44I GTGCAC 2 cut(s) 510, 2645
AlwI GGATC 7 cut(s) 255, 461, 570, 908, 921, 1580, 2753
AlwNI CAGNNNCTG 1 cut(s) 1091
Ama87I CYCGRG 1 cut(s) 2962
Aor13HI TCCGGA 1 cut(s) 910
AoxI GGCC 7 cut(s) 9, 164, 521, 976, 1030, 1368, 1800
ApaLI GTGCAC 2 cut(s) 510, 2645
ApeKI GCWGC 7 cut(s) 568, 596, 1151, 1163, 1481, 2222, 2225
ApoI RAATTY 6 cut(s) 199, 207, 541, 634, 1182, 1211
AseI ATTAAT 6 cut(s) 36, 999, 1613, 2342, 2346, 2814
Asp700I GAANNNNTTC 1 cut(s) 2883
AspLEI GCGC 1 cut(s) 573
AspS9I GGNCC 4 cut(s) 10, 619, 976, 1765
AsuHPI GGTGA 6 cut(s) 163, 404, 544, 1342, 2374, 2452
AvaI CYCGRG 1 cut(s) 2962
AvaII GGWCC 2 cut(s) 619, 1765
AxyI CCTNAGG 1 cut(s) 1590
BaeGI GKGCMC 4 cut(s) 514, 721, 2495, 2649
BamHI GGATCC 1 cut(s) 913
BanI GGYRCC 1 cut(s) 716
BanII GRGCYC 4 cut(s) 714, 2177, 2553, 2977
BauI CACGAG 1 cut(s) 2398
BbrPI CACGTG 1 cut(s) 2729
BbsI GAAGAC 3 cut(s) 1421, 2300, 2931
Bbv12I GWGCWC 5 cut(s) 514, 714, 2177, 2649, 2697
BbvI GCAGC 7 cut(s) 580, 583, 1138, 1150, 1493, 2212, 2234
BccI CCATC 2 cut(s) 742, 2866
BceAI ACGGC 2 cut(s) 872, 2772
BcgI CGANNNNNNTGC 2 cut(s) 2389, 2423
BciT130I CCWGG 7 cut(s) 507, 624, 726, 980, 1086, 2448, 2889
BclI TGATCA 2 cut(s) 2101, 2937
BcoDI GTCTC 4 cut(s) 95, 816, 869, 1626
BfaI CTAG 3 cut(s) 882, 2480, 2768
BfmI CTRYAG 1 cut(s) 366
BfrI CTTAAG 1 cut(s) 2038
BfuAI ACCTGC 2 cut(s) 1522, 2056
BglII AGATCT 5 cut(s) 1405, 1666, 2020, 2377, 2881
BisI GCNGC 9 cut(s) 569, 597, 1030, 1152, 1164, 1482, 2223, 2226, 2993
BlpI GCTNAGC 3 cut(s) 1155, 1491, 2319
BlsI GCNGC 9 cut(s) 570, 598, 1031, 1153, 1165, 1483, 2224, 2227, 2994
BmcAI AGTACT 1 cut(s) 1262
Bme1390I CCNGG 7 cut(s) 507, 624, 726, 980, 1086, 2448, 2889
Bme18I GGWCC 2 cut(s) 619, 1765
BmeT110I CYCGRG 1 cut(s) 2962
BmgBI CACGTC 2 cut(s) 1322, 2336
BmgT120I GGNCC 4 cut(s) 10, 619, 976, 1765
BmiI GGNNCC 6 cut(s) 621, 718, 915, 977, 1685, 2550
BmrFI CCNGG 7 cut(s) 507, 624, 726, 980, 1086, 2448, 2889
BmrI ACTGGG 2 cut(s) 788, 1409
BmsI GCATC 5 cut(s) 138, 608, 1795, 2320, 2620
BmuI ACTGGG 2 cut(s) 788, 1409
BpiI GAAGAC 3 cut(s) 1421, 2300, 2931
BplI GAGNNNNNCTC 2 cut(s) 1275, 1307
BpmI CTGGAG 2 cut(s) 246, 756
Bpu10I CCTNAGC 1 cut(s) 2073
Bpu1102I GCTNAGC 3 cut(s) 1155, 1491, 2319
BpuEI CTTGAG 2 cut(s) 1790, 2161
Bsa29I ATCGAT 1 cut(s) 132
BsaAI YACGTR 1 cut(s) 2729
BsaBI GATNNNNATC 2 cut(s) 1428, 2880
BsaWI WCCGGW 1 cut(s) 910
BsaXI ACNNNNNCTCC 4 cut(s) 1349, 1379, 1395, 1425
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 480, 616, 726, 2560
Bse1I ACTGG 7 cut(s) 383, 794, 1367, 1404, 1643, 2239, 2543
Bse21I CCTNAGG 1 cut(s) 1590
Bse8I GATNNNNATC 2 cut(s) 1428, 2880
BseAI TCCGGA 1 cut(s) 910
BseBI CCWGG 7 cut(s) 507, 624, 726, 980, 1086, 2448, 2889
BseCI ATCGAT 1 cut(s) 132
BseGI GGATG 3 cut(s) 1723, 2877, 2881
BseJI GATNNNNATC 2 cut(s) 1428, 2880
BseLI CCNNNNNNNGG 5 cut(s) 29, 480, 616, 726, 2560
BseNI ACTGG 7 cut(s) 383, 794, 1367, 1404, 1643, 2239, 2543
BseRI GAGGAG 1 cut(s) 175
BseSI GKGCMC 4 cut(s) 514, 721, 2495, 2649
BseXI GCAGC 7 cut(s) 580, 583, 1138, 1150, 1493, 2212, 2234
BseYI CCCAGC 3 cut(s) 720, 1235, 1857
BsgI GTGCAG 2 cut(s) 168, 2084
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 7 cut(s) 11, 166, 523, 978, 1032, 1370, 1802
BshNI GGYRCC 1 cut(s) 716
BshVI ATCGAT 1 cut(s) 132
BsiHKAI GWGCWC 5 cut(s) 514, 714, 2177, 2649, 2697
BsiHKCI CYCGRG 1 cut(s) 2962
BsiSI CCGG 2 cut(s) 305, 911
BslFI GGGAC 4 cut(s) 605, 1156, 1282, 2484
BslI CCNNNNNNNGG 5 cut(s) 29, 480, 616, 726, 2560
BsmAI GTCTC 4 cut(s) 95, 816, 869, 1626
BsmFI GGGAC 4 cut(s) 605, 1156, 1282, 2484
BsmI GAATGC 3 cut(s) 1391, 1748, 2458
BsnI GGCC 7 cut(s) 11, 166, 523, 978, 1032, 1370, 1802
BsoBI CYCGRG 1 cut(s) 2962
Bsp1286I GDGCHC 9 cut(s) 514, 714, 721, 2177, 2495, 2553, 2649, 2697, 2977
Bsp13I TCCGGA 1 cut(s) 910
Bsp1407I TGTACA 2 cut(s) 2394, 2638
Bsp1720I GCTNAGC 3 cut(s) 1155, 1491, 2319
BspANI GGCC 7 cut(s) 11, 166, 523, 978, 1032, 1370, 1802
BspDI ATCGAT 1 cut(s) 132
BspEI TCCGGA 1 cut(s) 910
BspFNI CGCG 1 cut(s) 176
BspHI TCATGA 1 cut(s) 3008
BspLI GGNNCC 6 cut(s) 621, 718, 915, 977, 1685, 2550
BspMI ACCTGC 2 cut(s) 1522, 2056
BspPI GGATC 7 cut(s) 255, 461, 570, 908, 921, 1580, 2753
BspT107I GGYRCC 1 cut(s) 716
BspTI CTTAAG 1 cut(s) 2038
BsrGI TGTACA 2 cut(s) 2394, 2638
BsrI ACTGG 7 cut(s) 383, 794, 1367, 1404, 1643, 2239, 2543
BssNAI GTATAC 1 cut(s) 373
BssSI CACGAG 1 cut(s) 2398
BssT1I CCWWGG 2 cut(s) 232, 2158
Bst1107I GTATAC 1 cut(s) 373
Bst2BI CACGAG 1 cut(s) 2398
Bst2UI CCWGG 7 cut(s) 507, 624, 726, 980, 1086, 2448, 2889
Bst6I CTCTTC 3 cut(s) 557, 1676, 2567
BstAFI CTTAAG 1 cut(s) 2038
BstAPI GCANNNNNTGC 3 cut(s) 596, 605, 1487
BstAUI TGTACA 2 cut(s) 2394, 2638
BstBAI YACGTR 1 cut(s) 2729
BstC8I GCNNGC 6 cut(s) 1034, 1053, 1161, 1746, 1890, 2850
BstDSI CCRYGG 3 cut(s) 174, 558, 2788
BstENI CCTNNNNNAGG 1 cut(s) 2558
BstF5I GGATG 3 cut(s) 1723, 2877, 2881
BstFNI CGCG 1 cut(s) 176
BstHHI GCGC 1 cut(s) 573
BstMAI GTCTC 4 cut(s) 95, 816, 869, 1626
BstNI CCWGG 7 cut(s) 507, 624, 726, 980, 1086, 2448, 2889
BstNSI RCATGY 6 cut(s) 65, 596, 704, 1748, 1949, 2645
BstSCI CCNGG 7 cut(s) 505, 622, 724, 978, 1084, 2446, 2887
BstSFI CTRYAG 1 cut(s) 366
BstSLI GKGCMC 4 cut(s) 514, 721, 2495, 2649
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 7 cut(s) 580, 583, 1138, 1150, 1493, 2212, 2234
BstV2I GAAGAC 3 cut(s) 1421, 2300, 2931
BstX2I RGATCY 7 cut(s) 913, 1405, 1585, 1666, 2020, 2377, 2881
BstYI RGATCY 7 cut(s) 913, 1405, 1585, 1666, 2020, 2377, 2881
BstZ17I GTATAC 1 cut(s) 373
Bsu15I ATCGAT 1 cut(s) 132
Bsu36I CCTNAGG 1 cut(s) 1590
BsuRI GGCC 7 cut(s) 11, 166, 523, 978, 1032, 1370, 1802
BsuTUI ATCGAT 1 cut(s) 132
BtgI CCRYGG 3 cut(s) 174, 558, 2788
BtrI CACGTC 2 cut(s) 1322, 2336
BtsCI GGATG 3 cut(s) 1723, 2877, 2881
BtsI GCAGTG 1 cut(s) 1396
BtsIMutI CAGTG 3 cut(s) 376, 1396, 2550
BveI ACCTGC 2 cut(s) 1522, 2056
Cac8I GCNNGC 6 cut(s) 1034, 1053, 1161, 1746, 1890, 2850
CaiI CAGNNNCTG 1 cut(s) 1091
CciI TCATGA 1 cut(s) 3008
CfoI GCGC 1 cut(s) 573
Cfr13I GGNCC 4 cut(s) 10, 619, 976, 1765
Cfr42I CCGCGG 1 cut(s) 177
ClaI ATCGAT 1 cut(s) 132
CseI GACGC 1 cut(s) 2294
CspCI CAANNNNNGTGG 4 cut(s) 1236, 1271, 2841, 2876
DraI TTTAAA 1 cut(s) 1725
EaeI YGGCCR 3 cut(s) 1030, 1368, 1800
Eam1104I CTCTTC 3 cut(s) 557, 1676, 2567
EarI CTCTTC 3 cut(s) 557, 1676, 2567
Ecl136II GAGCTC 2 cut(s) 712, 2175
Eco130I CCWWGG 2 cut(s) 232, 2158
Eco147I AGGCCT 1 cut(s) 166
Eco24I GRGCYC 4 cut(s) 714, 2177, 2553, 2977
Eco32I GATATC 1 cut(s) 2200
Eco47I GGWCC 2 cut(s) 619, 1765
Eco53kI GAGCTC 2 cut(s) 712, 2175
Eco57I CTGAAG 3 cut(s) 1661, 2036, 2517
Eco72I CACGTG 1 cut(s) 2729
Eco81I CCTNAGG 1 cut(s) 1590
Eco88I CYCGRG 1 cut(s) 2962
EcoICRI GAGCTC 2 cut(s) 712, 2175
EcoNI CCTNNNNNAGG 1 cut(s) 2558
EcoO109I RGGNCCY 3 cut(s) 10, 619, 1765
EcoRI GAATTC 3 cut(s) 199, 634, 1182
EcoRII CCWGG 7 cut(s) 505, 622, 724, 978, 1084, 2446, 2887
EcoRV GATATC 1 cut(s) 2200
EcoT14I CCWWGG 2 cut(s) 232, 2158
EcoT22I ATGCAT 2 cut(s) 1750, 2111
EcoT38I GRGCYC 4 cut(s) 714, 2177, 2553, 2977
ErhI CCWWGG 2 cut(s) 232, 2158
FalI AAGNNNNNCTT 8 cut(s) 744, 776, 809, 841, 1863, 1895, 2291, 2323
FaqI GGGAC 4 cut(s) 605, 1156, 1282, 2484
FauI CCCGC 1 cut(s) 966
FauNDI CATATG 2 cut(s) 89, 2137
FbaI TGATCA 2 cut(s) 2101, 2937
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 9 cut(s) 569, 597, 1030, 1152, 1164, 1482, 2223, 2226, 2993
FokI GGATG 3 cut(s) 1730, 2884, 2888
FriOI GRGCYC 4 cut(s) 714, 2177, 2553, 2977
Fsp4HI GCNGC 9 cut(s) 569, 597, 1030, 1152, 1164, 1482, 2223, 2226, 2993
FspBI CTAG 3 cut(s) 882, 2480, 2768
GlaI GCGC 1 cut(s) 572
GluI GCNGC 9 cut(s) 569, 597, 1030, 1152, 1164, 1482, 2223, 2226, 2993
GsaI CCCAGC 3 cut(s) 724, 1239, 1861
GsuI CTGGAG 2 cut(s) 246, 756
HaeIII GGCC 7 cut(s) 11, 166, 523, 978, 1032, 1370, 1802
HapII CCGG 2 cut(s) 305, 911
HgaI GACGC 1 cut(s) 2294
HhaI GCGC 1 cut(s) 573
Hin6I GCGC 1 cut(s) 571
HinP1I GCGC 1 cut(s) 571
HincII GTYRAC 1 cut(s) 2035
HindII GTYRAC 1 cut(s) 2035
HindIII AAGCTT 1 cut(s) 845
HpaII CCGG 2 cut(s) 305, 911
HphI GGTGA 6 cut(s) 163, 404, 544, 1342, 2374, 2452
HpyCH4IV ACGT 7 cut(s) 53, 1321, 1927, 2335, 2430, 2728, 2755
HpySE526I ACGT 7 cut(s) 53, 1321, 1927, 2335, 2430, 2728, 2755
HspAI GCGC 1 cut(s) 571
Kpn2I TCCGGA 1 cut(s) 910
Ksp22I TGATCA 2 cut(s) 2101, 2937
KspI CCGCGG 1 cut(s) 177
LmnI GCTCC 8 cut(s) 307, 508, 1048, 2254, 2328, 2461, 2548, 2608
Lsp1109I GCAGC 7 cut(s) 580, 583, 1138, 1150, 1493, 2212, 2234
LweI GCATC 5 cut(s) 138, 608, 1795, 2320, 2620
MaeI CTAG 3 cut(s) 882, 2480, 2768
MaeII ACGT 7 cut(s) 53, 1321, 1927, 2335, 2430, 2728, 2755
MfeI CAATTG 3 cut(s) 384, 527, 1011
MflI RGATCY 7 cut(s) 913, 1405, 1585, 1666, 2020, 2377, 2881
MhlI GDGCHC 9 cut(s) 514, 714, 721, 2177, 2495, 2553, 2649, 2697, 2977
MlyI GAGTC 3 cut(s) 173, 237, 1178
MmeI TCCRAC 2 cut(s) 928, 1986
Mph1103I ATGCAT 2 cut(s) 1750, 2111
MroI TCCGGA 1 cut(s) 910
MroXI GAANNNNTTC 1 cut(s) 2883
MslI CAYNNNNRTG 2 cut(s) 272, 806
MspA1I CMGCKG 4 cut(s) 176, 462, 1029, 2225
MspCI CTTAAG 1 cut(s) 2038
MspI CCGG 2 cut(s) 305, 911
MspR9I CCNGG 7 cut(s) 507, 624, 726, 980, 1086, 2448, 2889
MunI CAATTG 3 cut(s) 384, 527, 1011
Mva1269I GAATGC 3 cut(s) 1391, 1748, 2458
MvaI CCWGG 7 cut(s) 507, 624, 726, 980, 1086, 2448, 2889
MvnI CGCG 1 cut(s) 176
NdeI CATATG 2 cut(s) 89, 2137
NlaIV GGNNCC 6 cut(s) 621, 718, 915, 977, 1685, 2550
NmeAIII GCCGAG 1 cut(s) 1396
NmuCI GTSAC 6 cut(s) 355, 1317, 1783, 1962, 2798, 2818
NsiI ATGCAT 2 cut(s) 1750, 2111
NspI RCATGY 6 cut(s) 65, 596, 704, 1748, 1949, 2645
PacI TTAATTAA 1 cut(s) 2346
PaeI GCATGC 1 cut(s) 1748
PagI TCATGA 1 cut(s) 3008
PasI CCCWGGG 2 cut(s) 623, 2447
PceI AGGCCT 1 cut(s) 166
PciI ACATGT 2 cut(s) 61, 2641
PctI GAATGC 3 cut(s) 1391, 1748, 2458
PdmI GAANNNNTTC 1 cut(s) 2883
PfeI GAWTC 9 cut(s) 74, 193, 395, 1096, 1304, 2270, 2518, 2578, 2723
PkrI GCNGC 9 cut(s) 570, 598, 1031, 1153, 1165, 1483, 2224, 2227, 2994
PleI GAGTC 3 cut(s) 173, 236, 1177
PmaCI CACGTG 1 cut(s) 2729
PmlI CACGTG 1 cut(s) 2729
PpsI GAGTC 3 cut(s) 173, 236, 1177
Ppu21I YACGTR 1 cut(s) 2729
PpuMI RGGWCCY 2 cut(s) 619, 1765
PscI ACATGT 2 cut(s) 61, 2641
PshBI ATTAAT 6 cut(s) 36, 999, 1613, 2342, 2346, 2814
PsiI TTATAA 1 cut(s) 452
Psp124BI GAGCTC 2 cut(s) 714, 2177
Psp1406I AACGTT 1 cut(s) 53
Psp5II RGGWCCY 2 cut(s) 619, 1765
Psp6I CCWGG 7 cut(s) 505, 622, 724, 978, 1084, 2446, 2887
PspCI CACGTG 1 cut(s) 2729
PspFI CCCAGC 3 cut(s) 720, 1235, 1857
PspGI CCWGG 7 cut(s) 505, 622, 724, 978, 1084, 2446, 2887
PspN4I GGNNCC 6 cut(s) 621, 718, 915, 977, 1685, 2550
PspPI GGNCC 4 cut(s) 10, 619, 976, 1765
PspPPI RGGWCCY 2 cut(s) 619, 1765
PstNI CAGNNNCTG 1 cut(s) 1091
PsuI RGATCY 7 cut(s) 913, 1405, 1585, 1666, 2020, 2377, 2881
PvuII CAGCTG 1 cut(s) 2225
RseI CAYNNNNRTG 2 cut(s) 272, 806
SacI GAGCTC 2 cut(s) 714, 2177
SacII CCGCGG 1 cut(s) 177
SatI GCNGC 9 cut(s) 569, 597, 1030, 1152, 1164, 1482, 2223, 2226, 2993
Sau96I GGNCC 4 cut(s) 10, 619, 976, 1765
ScaI AGTACT 1 cut(s) 1262
SchI GAGTC 3 cut(s) 173, 237, 1178
ScrFI CCNGG 7 cut(s) 507, 624, 726, 980, 1086, 2448, 2889
SduI GDGCHC 9 cut(s) 514, 714, 721, 2177, 2495, 2553, 2649, 2697, 2977
SfaNI GCATC 5 cut(s) 138, 608, 1795, 2320, 2620
SfcI CTRYAG 1 cut(s) 366
Sfr303I CCGCGG 1 cut(s) 177
SgrBI CCGCGG 1 cut(s) 177
SinI GGWCC 2 cut(s) 619, 1765
SmiMI CAYNNNNRTG 2 cut(s) 272, 806
SmlI CTYRAG 3 cut(s) 1769, 2038, 2176
SmoI CTYRAG 3 cut(s) 1769, 2038, 2176
SphI GCATGC 1 cut(s) 1748
SseBI AGGCCT 1 cut(s) 166
SspI AATATT 3 cut(s) 261, 2081, 2744
SspMI CTAG 3 cut(s) 882, 2480, 2768
SstI GAGCTC 2 cut(s) 714, 2177
StuI AGGCCT 1 cut(s) 166
StyD4I CCNGG 7 cut(s) 505, 622, 724, 978, 1084, 2446, 2887
StyI CCWWGG 2 cut(s) 232, 2158
TaiI ACGT 7 cut(s) 56, 1324, 1930, 2338, 2433, 2731, 2758
TaqI TCGA 5 cut(s) 132, 270, 1383, 1579, 1991
TatI WGTACW 9 cut(s) 84, 819, 1140, 1260, 2148, 2211, 2394, 2638, 2918
TauI GCSGC 2 cut(s) 1032, 2995
TfiI GAWTC 9 cut(s) 74, 193, 395, 1096, 1304, 2270, 2518, 2578, 2723
TscAI CASTG 3 cut(s) 383, 1396, 2550
TseFI GTSAC 6 cut(s) 355, 1317, 1783, 1962, 2798, 2818
TseI GCWGC 7 cut(s) 568, 596, 1151, 1163, 1481, 2222, 2225
Tsp45I GTSAC 6 cut(s) 355, 1317, 1783, 1962, 2798, 2818
TspGWI ACGGA 2 cut(s) 547, 947
TspRI CASTG 3 cut(s) 383, 1396, 2550
Vha464I CTTAAG 1 cut(s) 2038
VneI GTGCAC 2 cut(s) 510, 2645
VpaK11BI GGWCC 2 cut(s) 619, 1765
VspI ATTAAT 6 cut(s) 36, 999, 1613, 2342, 2346, 2814
XagI CCTNNNNNAGG 1 cut(s) 2558
XapI RAATTY 6 cut(s) 199, 207, 541, 634, 1182, 1211
XceI RCATGY 6 cut(s) 65, 596, 704, 1748, 1949, 2645
XmiI GTMKAC 1 cut(s) 372
XmnI GAANNNNTTC 1 cut(s) 2883
XspI CTAG 3 cut(s) 882, 2480, 2768
ZrmI AGTACT 1 cut(s) 1262
Zsp2I ATGCAT 2 cut(s) 1750, 2111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.