pycom09g00650

Ribosomal RNA-processing protein 7 (RRP7)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002008
Physical Location & Seq
Forward (+)
485598 .. 488513
2916 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGAAGGACAAGAAGAAGACCCATAAGAGCAAGAAGGCTGCCCAGCCAAATTTGGAGATTGTTGAAAAGGATAAAATGTTAGGATCGGCAGAGATGAGAAAGAGAAAAAGGGCGAAGGAAAGCGATGCTGACTCTCAAAAGGTTGTCCAAGTCTCTGTTGAAGTAGATAATTCAGCCAAATCAGCCAAAGATAAGAAAAAGACAAAACTAACGAAGAAAAGGAAAATAAAGAATGCGAAGAATTATGTAGCTGTGGAAAGCGAGAATGATCACCCAAATACGGAGACTGATGGCGATTGTAATGAAATAAATGATGATGGAGTTGCCAATCAGAGCCGATTCGAAACTCAGGAAGAGATTGAAATTGGTGAAGTGTTCACTGAGGCTGGTAAGTCAAAGAAAGCTAAGAAAAAGAAAAAGAAGGATCGTAATCCATTAGAGTTTGTGAAGTCACTGGAAAAGGAAATAGAAGCTGGTCGGGAGATCTGCTCAAGAGGAATACCAAGCGTGTCCAAGAAAGCTTTGAAAAAGAAAAAGAGGGATCTTGATTCATCAAAGTCCACAAAGACAATGGAAACGGAAGTAGAAGCTGATCAGGCTGATGTTTACCTTATTTCTTCGGGGGATGAGGACTCTTCGAAAGGAATGAAAAAATGGGTTACGGAATACCATCAAAGTAGACCTGGATTGAAGGAACTGCAGCAAAGAATCGATCAGTTTATGGTTGAACATGATGAAAAACTGGAACAGGAAAAGAAAGAGAGAGAAGCCCGTGCTGCAGAGGGAGGATGGACAGTTGTAGTACATCATAAAGGAAGGAAGAAGACCACGGATGCTGAGAGTGGAATTACAGTAGGGTCTGTCGCCCAGGCTGCTTTGGAGGACAAGATGGCCAAGAAGAAACAAACTGAAGTTGTTGGGCTAGATTTTTACCGGTTCCAAAGGAAAGAAGCTCAGAGGAATGAAATTATGATGCTCCAGAGCAAGTTTGAGCAAGATAAAAAGCGGATACAGCAGTTAAGAGCGGCTAGGAAGTTTCGACCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
RRP7

Protein Analysis

350

Amino Acids

39.96

Weight (kDa)

9.54

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRP7 PF12923 225 - 349 1e-27 Ribosomal RNA-processing protein 7 (RRP7) C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1025
AccI GTMKAC 1 cut(s) 679
AciI CCGC 2 cut(s) 1006, 1025
AclWI GGATC 3 cut(s) 91, 432, 549
AcoI YGGCCR 1 cut(s) 891
AcsI RAATTY 1 cut(s) 49
AcuI CTGAAG 1 cut(s) 930
AfaI GTAC 1 cut(s) 804
AfiI CCNNNNNNNGG 1 cut(s) 280
AgeI ACCGGT 1 cut(s) 933
AgsI TTSAA 6 cut(s) 65, 161, 362, 526, 691, 728
AjnI CCWGG 2 cut(s) 682, 867
AluBI AGCT 6 cut(s) 251, 404, 473, 521, 590, 953
AluI AGCT 6 cut(s) 251, 404, 473, 521, 590, 953
Alw26I GTCTC 2 cut(s) 157, 278
AlwI GGATC 3 cut(s) 91, 432, 549
AoxI GGCC 1 cut(s) 891
ApeKI GCWGC 4 cut(s) 38, 700, 776, 872
ApoI RAATTY 1 cut(s) 49
AsiGI ACCGGT 1 cut(s) 933
AsuHPI GGTGA 2 cut(s) 263, 380
AsuII TTCGAA 2 cut(s) 342, 638
BaeI ACNNNNGTAYC 2 cut(s) 1001, 1034
BalI TGGCCA 1 cut(s) 893
BbsI GAAGAC 2 cut(s) 23, 830
BbvI GCAGC 4 cut(s) 25, 712, 763, 859
BccI CCATC 5 cut(s) 284, 311, 678, 783, 883
BciT130I CCWGG 2 cut(s) 684, 869
BciVI GTATCC 1 cut(s) 1002
BclI TGATCA 2 cut(s) 268, 592
BcoDI GTCTC 2 cut(s) 157, 278
BfaI CTAG 2 cut(s) 923, 1029
BfmI CTRYAG 2 cut(s) 698, 777
BfuI GTATCC 1 cut(s) 1002
BglII AGATCT 1 cut(s) 483
BisI GCNGC 5 cut(s) 39, 701, 777, 873, 1026
BlsI GCNGC 5 cut(s) 40, 702, 778, 874, 1027
Bme1390I CCNGG 2 cut(s) 684, 869
BmiI GGNNCC 1 cut(s) 938
BmrFI CCNGG 2 cut(s) 684, 869
BmsI GCATC 3 cut(s) 115, 823, 963
BpiI GAAGAC 2 cut(s) 23, 830
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpmI CTGGAG 1 cut(s) 962
Bpu14I TTCGAA 2 cut(s) 342, 638
BpuEI CTTGAG 1 cut(s) 475
Bsa29I ATCGAT 1 cut(s) 711
BsaBI GATNNNNATC 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 828, 867
BsaWI WCCGGW 1 cut(s) 933
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse118I RCCGGY 1 cut(s) 933
Bse1I ACTGG 2 cut(s) 459, 747
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 2 cut(s) 684, 869
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 2 cut(s) 828, 867
BseGI GGATG 3 cut(s) 631, 794, 838
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMII CTCAG 4 cut(s) 362, 372, 828, 968
BseNI ACTGG 2 cut(s) 459, 747
BseXI GCAGC 4 cut(s) 25, 712, 763, 859
BseYI CCCAGC 1 cut(s) 42
BshFI GGCC 1 cut(s) 893
BshTI ACCGGT 1 cut(s) 933
BshVI ATCGAT 1 cut(s) 711
BsiSI CCGG 1 cut(s) 934
BslI CCNNNNNNNGG 1 cut(s) 280
BsmAI GTCTC 2 cut(s) 157, 278
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 1 cut(s) 893
Bsp119I TTCGAA 2 cut(s) 342, 638
Bsp143I GATC 7 cut(s) 83, 268, 424, 483, 541, 592, 712
BspACI CCGC 2 cut(s) 1006, 1025
BspANI GGCC 1 cut(s) 893
BspCNI CTCAG 4 cut(s) 361, 373, 829, 967
BspDI ATCGAT 1 cut(s) 711
BspLI GGNNCC 1 cut(s) 938
BspMAI CTGCAG 2 cut(s) 702, 781
BspPI GGATC 3 cut(s) 91, 432, 549
BspT104I TTCGAA 2 cut(s) 342, 638
BsrBI CCGCTC 1 cut(s) 1025
BsrFI RCCGGY 1 cut(s) 933
BsrI ACTGG 2 cut(s) 459, 747
BssAI RCCGGY 1 cut(s) 933
BssECI CCNNGG 2 cut(s) 828, 867
BssMI GATC 7 cut(s) 83, 268, 424, 483, 541, 592, 712
Bst2UI CCWGG 2 cut(s) 684, 869
Bst4CI ACNGT 2 cut(s) 796, 853
Bst6I CTCTTC 2 cut(s) 348, 640
BstBI TTCGAA 2 cut(s) 342, 638
BstDEI CTNAG 5 cut(s) 348, 381, 405, 837, 954
BstDSI CCRYGG 1 cut(s) 828
BstF5I GGATG 3 cut(s) 631, 794, 838
BstKTI GATC 7 cut(s) 86, 271, 427, 486, 544, 595, 715
BstMAI GTCTC 2 cut(s) 157, 278
BstMBI GATC 7 cut(s) 83, 268, 424, 483, 541, 592, 712
BstMWI GCNNNNNNNGC 5 cut(s) 182, 596, 776, 872, 1012
BstNI CCWGG 2 cut(s) 684, 869
BstSCI CCNGG 2 cut(s) 682, 867
BstSFI CTRYAG 2 cut(s) 698, 777
BstV1I GCAGC 4 cut(s) 25, 712, 763, 859
BstV2I GAAGAC 2 cut(s) 23, 830
BstX2I RGATCY 2 cut(s) 483, 541
BstYI RGATCY 2 cut(s) 483, 541
Bsu15I ATCGAT 1 cut(s) 711
BsuI GTATCC 1 cut(s) 1002
BsuRI GGCC 1 cut(s) 893
BsuTUI ATCGAT 1 cut(s) 711
BtgI CCRYGG 1 cut(s) 828
BtgZI GCGATG 1 cut(s) 138
BtsCI GGATG 3 cut(s) 631, 794, 838
BtsIMutI CAGTG 2 cut(s) 378, 452
Cfr10I RCCGGY 1 cut(s) 933
ClaI ATCGAT 1 cut(s) 711
Csp6I GTAC 1 cut(s) 803
CspAI ACCGGT 1 cut(s) 933
CviAII CATG 1 cut(s) 731
CviQI GTAC 1 cut(s) 803
DdeI CTNAG 5 cut(s) 348, 381, 405, 837, 954
DpnI GATC 7 cut(s) 85, 270, 426, 485, 543, 594, 714
DpnII GATC 7 cut(s) 83, 268, 424, 483, 541, 592, 712
EaeI YGGCCR 1 cut(s) 891
Eam1104I CTCTTC 2 cut(s) 348, 640
EarI CTCTTC 2 cut(s) 348, 640
Eco57I CTGAAG 1 cut(s) 930
EcoRII CCWGG 2 cut(s) 682, 867
FaeI CATG 1 cut(s) 734
FaiI YATR 6 cut(s) 24, 246, 722, 732, 810, 971
FatI CATG 1 cut(s) 730
FbaI TGATCA 2 cut(s) 268, 592
FblI GTMKAC 1 cut(s) 679
Fnu4HI GCNGC 5 cut(s) 39, 701, 777, 873, 1026
FokI GGATG 3 cut(s) 638, 801, 845
Fsp4HI GCNGC 5 cut(s) 39, 701, 777, 873, 1026
FspBI CTAG 2 cut(s) 923, 1029
GluI GCNGC 5 cut(s) 39, 701, 777, 873, 1026
GsaI CCCAGC 1 cut(s) 46
GsuI CTGGAG 1 cut(s) 962
HaeIII GGCC 1 cut(s) 893
HapII CCGG 1 cut(s) 934
Hin1II CATG 1 cut(s) 734
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 5 cut(s) 131, 339, 548, 632, 708
HpaII CCGG 1 cut(s) 934
HphI GGTGA 2 cut(s) 263, 380
Hpy166II GTNNAC 4 cut(s) 378, 561, 607, 680
Hpy188I TCNGA 2 cut(s) 333, 957
Hpy188III TCNNGA 5 cut(s) 350, 479, 492, 545, 979
Hpy8I GTNNAC 4 cut(s) 378, 561, 607, 680
HpyAV CCTTC 5 cut(s) 28, 109, 415, 685, 810
HpyCH4III ACNGT 2 cut(s) 796, 853
HpyCH4V TGCA 2 cut(s) 700, 779
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 596, 776, 872, 1012
HpyF3I CTNAG 5 cut(s) 348, 381, 405, 837, 954
Hsp92II CATG 1 cut(s) 734
Ksp22I TGATCA 2 cut(s) 268, 592
Kzo9I GATC 7 cut(s) 83, 268, 424, 483, 541, 592, 712
LmnI GCTCC 1 cut(s) 981
Lsp1109I GCAGC 4 cut(s) 25, 712, 763, 859
LweI GCATC 3 cut(s) 115, 823, 963
MaeI CTAG 2 cut(s) 923, 1029
MaeIII GTNAC 2 cut(s) 450, 658
MalI GATC 7 cut(s) 85, 270, 426, 485, 543, 594, 714
MbiI CCGCTC 1 cut(s) 1025
MboI GATC 7 cut(s) 83, 268, 424, 483, 541, 592, 712
MflI RGATCY 2 cut(s) 483, 541
MlsI TGGCCA 1 cut(s) 893
MluCI AATT 6 cut(s) 49, 169, 241, 363, 846, 966
MluNI TGGCCA 1 cut(s) 893
MlyI GAGTC 2 cut(s) 125, 626
MnlI CCTC 8 cut(s) 376, 488, 531, 622, 775, 779, 874, 951
Mox20I TGGCCA 1 cut(s) 893
MscI TGGCCA 1 cut(s) 893
MseI TTAA 1 cut(s) 1019
Msp20I TGGCCA 1 cut(s) 893
MspI CCGG 1 cut(s) 934
MspR9I CCNGG 2 cut(s) 684, 869
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 2 cut(s) 684, 869
MwoI GCNNNNNNNGC 5 cut(s) 182, 596, 776, 872, 1012
NdeII GATC 7 cut(s) 83, 268, 424, 483, 541, 592, 712
NlaIII CATG 1 cut(s) 734
NlaIV GGNNCC 1 cut(s) 938
NmuCI GTSAC 1 cut(s) 450
NspV TTCGAA 2 cut(s) 342, 638
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 3 cut(s) 339, 548, 708
PinAI ACCGGT 1 cut(s) 933
PkrI GCNGC 5 cut(s) 40, 702, 778, 874, 1027
PleI GAGTC 2 cut(s) 125, 626
PpsI GAGTC 2 cut(s) 125, 626
Psp6I CCWGG 2 cut(s) 682, 867
PspFI CCCAGC 1 cut(s) 42
PspGI CCWGG 2 cut(s) 682, 867
PspN4I GGNNCC 1 cut(s) 938
PstI CTGCAG 2 cut(s) 702, 781
PsuI RGATCY 2 cut(s) 483, 541
RsaI GTAC 1 cut(s) 804
RsaNI GTAC 1 cut(s) 803
SaqAI TTAA 1 cut(s) 1019
SatI GCNGC 5 cut(s) 39, 701, 777, 873, 1026
Sau3AI GATC 7 cut(s) 83, 268, 424, 483, 541, 592, 712
SchI GAGTC 2 cut(s) 125, 626
ScrFI CCNGG 2 cut(s) 684, 869
SfaNI GCATC 3 cut(s) 115, 823, 963
SfcI CTRYAG 2 cut(s) 698, 777
SfuI TTCGAA 2 cut(s) 342, 638
SmlI CTYRAG 1 cut(s) 490
SmoI CTYRAG 1 cut(s) 490
Sse9I AATT 6 cut(s) 49, 169, 241, 363, 846, 966
SsiI CCGC 2 cut(s) 1006, 1025
SspMI CTAG 2 cut(s) 923, 1029
StyD4I CCNGG 2 cut(s) 682, 867
TaaI ACNGT 2 cut(s) 796, 853
TaqI TCGA 4 cut(s) 342, 638, 711, 1039
TasI AATT 6 cut(s) 49, 169, 241, 363, 846, 966
TatI WGTACW 1 cut(s) 802
TauI GCSGC 1 cut(s) 1028
TfiI GAWTC 3 cut(s) 339, 548, 708
Tru1I TTAA 1 cut(s) 1019
Tru9I TTAA 1 cut(s) 1019
TscAI CASTG 2 cut(s) 385, 459
TseFI GTSAC 1 cut(s) 450
TseI GCWGC 4 cut(s) 38, 700, 776, 872
Tsp45I GTSAC 1 cut(s) 450
TspDTI ATGAA 6 cut(s) 17, 318, 540, 662, 750, 978
TspGWI ACGGA 4 cut(s) 296, 593, 677, 845
TspRI CASTG 2 cut(s) 385, 459
XapI RAATTY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 568
XmiI GTMKAC 1 cut(s) 679
XspI CTAG 2 cut(s) 923, 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.