pycom10g01730

Acylpyruvase FAHD1, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002365
Physical Location & Seq
Forward (+)
1823276 .. 1825164
1889 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 426 bp
ATGAACAGCGGCACGATCGATGTCTCTCACCCTCTGTTATCGTTGGACCATGAGGTGGCGGTGATCATCTCTCAGAAAGCTCGCGATGTTCCGCAATCCACCGCCATGGATTACGTTGCGGGTTATGCACTTGCTTTGGATATGACTGCCAGAGAAATTCAAGCTTCTGCTAAGATAAAATCTACAGAGATTAGAAAGAGAAAAAGGGCAAAGGAAAGCTATGGTGACTCTCGAAAGATGGTTCAAGTCTCTGTTGGAGGAGAGAATCAGACCATTGAGTGGGCTATACGACGAACAGGTGCTCTGAACATTGCCAAGGCATTAGATCACTGCAGCAGCGAATGTTGCCCATTGTACCATGACTTAAATGCATATATGGTTGTCTTTGATAAGAGTTGGCTTCATTTCAGTCCAGTAACTCTCTAA

Protein Analysis

142

Amino Acids

15.67

Weight (kDa)

7.75

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAA_hydrolase PF01557 16 - 88 2.8e-12 Fumarylacetoacetate (FAA) hydrolase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 55, 279
AccII CGCG 1 cut(s) 84
AciI CCGC 5 cut(s) 9, 59, 92, 102, 119
AcsI RAATTY 1 cut(s) 156
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 2 cut(s) 55, 279
AgsI TTSAA 2 cut(s) 161, 245
AluBI AGCT 3 cut(s) 80, 164, 219
AluI AGCT 3 cut(s) 80, 164, 219
Alw21I GWGCWC 1 cut(s) 304
Alw26I GTCTC 2 cut(s) 28, 253
ApeKI GCWGC 2 cut(s) 333, 336
ApoI RAATTY 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 46
AsuHPI GGTGA 3 cut(s) 20, 73, 236
AvaII GGWCC 1 cut(s) 46
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 2 cut(s) 345, 348
BccI CCATC 1 cut(s) 232
BclI TGATCA 1 cut(s) 63
BcoDI GTCTC 2 cut(s) 28, 253
BfmI CTRYAG 2 cut(s) 183, 331
BisI GCNGC 3 cut(s) 10, 334, 337
BlsI GCNGC 3 cut(s) 11, 335, 338
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 46
Bsa29I ATCGAT 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 105, 315
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 279
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 309
BseCI ATCGAT 1 cut(s) 18
BseDI CCNNGG 2 cut(s) 105, 315
BseLI CCNNNNNNNGG 2 cut(s) 55, 279
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 1 cut(s) 413
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 2 cut(s) 345, 348
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 18
BshVI ATCGAT 1 cut(s) 18
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 304
BslI CCNNNNNNNGG 2 cut(s) 55, 279
BsmAI GTCTC 2 cut(s) 28, 253
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 3 cut(s) 15, 63, 325
Bsp19I CCATGG 1 cut(s) 105
Bsp68I TCGCGA 1 cut(s) 84
BspACI CCGC 5 cut(s) 9, 59, 92, 102, 119
BspCNI CTCAG 1 cut(s) 85
BspDI ATCGAT 1 cut(s) 18
BspFNI CGCG 1 cut(s) 84
BspMAI CTGCAG 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 309
BsrI ACTGG 1 cut(s) 413
BssECI CCNNGG 2 cut(s) 105, 315
BssMI GATC 3 cut(s) 15, 63, 325
BssT1I CCWWGG 2 cut(s) 105, 315
BstC8I GCNNGC 1 cut(s) 82
BstDEI CTNAG 2 cut(s) 72, 171
BstDSI CCRYGG 1 cut(s) 105
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 3 cut(s) 18, 66, 328
BstMAI GTCTC 2 cut(s) 28, 253
BstMBI GATC 3 cut(s) 15, 63, 325
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 2 cut(s) 125, 345
BstSFI CTRYAG 2 cut(s) 183, 331
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 345, 348
BstXI CCANNNNNNTGG 1 cut(s) 106
Bsu15I ATCGAT 1 cut(s) 18
BsuTUI ATCGAT 1 cut(s) 18
BtgI CCRYGG 1 cut(s) 105
BtgZI GCGATG 1 cut(s) 99
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 1 cut(s) 328
BtuMI TCGCGA 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 46
ClaI ATCGAT 1 cut(s) 18
Csp6I GTAC 1 cut(s) 355
CviAII CATG 3 cut(s) 50, 106, 359
CviJI RGCY 5 cut(s) 80, 164, 219, 284, 400
CviKI_1 RGCY 5 cut(s) 80, 164, 219, 284, 400
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 2 cut(s) 72, 171
DpnI GATC 3 cut(s) 17, 65, 327
DpnII GATC 3 cut(s) 15, 63, 325
Eco130I CCWWGG 2 cut(s) 105, 315
Eco47I GGWCC 1 cut(s) 46
EcoT14I CCWWGG 2 cut(s) 105, 315
EcoT22I ATGCAT 1 cut(s) 373
ErhI CCWWGG 2 cut(s) 105, 315
FaeI CATG 3 cut(s) 53, 109, 362
FatI CATG 3 cut(s) 49, 105, 358
FauI CCCGC 1 cut(s) 112
FbaI TGATCA 1 cut(s) 63
Fnu4HI GCNGC 3 cut(s) 10, 334, 337
Fsp4HI GCNGC 3 cut(s) 10, 334, 337
GluI GCNGC 3 cut(s) 10, 334, 337
Hin1II CATG 3 cut(s) 53, 109, 362
HindIII AAGCTT 1 cut(s) 162
HinfI GANTC 2 cut(s) 227, 265
HphI GGTGA 3 cut(s) 20, 73, 236
Hpy188I TCNGA 3 cut(s) 75, 270, 306
Hpy188III TCNNGA 2 cut(s) 83, 231
Hpy99I CGWCG 1 cut(s) 294
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 3 cut(s) 128, 333, 371
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 345
HpyF3I CTNAG 2 cut(s) 72, 171
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 3 cut(s) 53, 109, 362
Ksp22I TGATCA 1 cut(s) 63
Kzo9I GATC 3 cut(s) 15, 63, 325
LpnPI CCDG 2 cut(s) 163, 282
Lsp1109I GCAGC 2 cut(s) 345, 348
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 2 cut(s) 224, 415
MalI GATC 3 cut(s) 17, 65, 327
MboI GATC 3 cut(s) 15, 63, 325
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 1 cut(s) 156
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 2 cut(s) 24, 235
MnlI CCTC 3 cut(s) 42, 46, 251
Mph1103I ATGCAT 1 cut(s) 373
MseI TTAA 1 cut(s) 365
MslI CAYNNNNRTG 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 9
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 2 cut(s) 125, 345
NcoI CCATGG 1 cut(s) 105
NdeII GATC 3 cut(s) 15, 63, 325
NlaIII CATG 3 cut(s) 53, 109, 362
NmuCI GTSAC 1 cut(s) 224
NruI TCGCGA 1 cut(s) 84
NsiI ATGCAT 1 cut(s) 373
PfeI GAWTC 1 cut(s) 265
PflMI CCANNNNNTGG 2 cut(s) 55, 279
PkrI GCNGC 3 cut(s) 11, 335, 338
Ple19I CGATCG 1 cut(s) 18
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PspPI GGNCC 1 cut(s) 46
PstI CTGCAG 1 cut(s) 335
PvuI CGATCG 1 cut(s) 18
RruI TCGCGA 1 cut(s) 84
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 1 cut(s) 365
SatI GCNGC 3 cut(s) 10, 334, 337
Sau3AI GATC 3 cut(s) 15, 63, 325
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 221
SduI GDGCHC 1 cut(s) 304
SetI ASST 6 cut(s) 57, 82, 117, 166, 221, 301
SfcI CTRYAG 2 cut(s) 183, 331
SinI GGWCC 1 cut(s) 46
SmiMI CAYNNNNRTG 1 cut(s) 104
Sse9I AATT 1 cut(s) 156
SsiI CCGC 5 cut(s) 9, 59, 92, 102, 119
StyI CCWWGG 2 cut(s) 105, 315
TaiI ACGT 1 cut(s) 117
TaqI TCGA 2 cut(s) 18, 232
TasI AATT 1 cut(s) 156
TauI GCSGC 1 cut(s) 12
TfiI GAWTC 1 cut(s) 265
Tru1I TTAA 1 cut(s) 365
Tru9I TTAA 1 cut(s) 365
TscAI CASTG 1 cut(s) 335
TseFI GTSAC 1 cut(s) 224
TseI GCWGC 2 cut(s) 333, 336
Tsp45I GTSAC 1 cut(s) 224
TspDTI ATGAA 2 cut(s) 17, 392
TspRI CASTG 1 cut(s) 335
Van91I CCANNNNNTGG 2 cut(s) 55, 279
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 1 cut(s) 156
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.