pycom10g06750

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002770
Physical Location & Seq
Reverse (-)
7937262 .. 7940309
3048 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
ATGGAATTCGTTGCTGAACAAGGCAAACACGCCCATGGTGGCTCTTCCACTCTGATCCTGCCAGCTTTGTCAATTGGAAATGTGGGGCAGCTTGCGGTGGACCTTCTAGTTGCATCAATGAAAGCAGAGAGAGTTGGGTATTTGGACACTCCATTTGTGGTTCCTTGTGTCGGCAATGATGCCTATGGGCCTGTTCCTAATGGGGAGCTTGCTCTCCCTCTTGAAGCTTATGATTCATCTTCCAATGCAATGTCTCTAATCCAACAAAGGTCCCCTGTTGTAAAGGGAAGGATGATTGAATTTGCTAAATGTGTGGCTGATTATGTTGCTGCTAGTGGAAAGAAGAATGTTGTTGTGCTCTCGAGCTTGGATTTTGGGAGGTGGCAGAGAGTTGATATGTCAAGTGGTTTGCAGATGCATTACCTGTCTAGCACCAATTCAGATGGAACAGATGATTATTGTGAACAGCTTGGGTGGAAAAGGCTGCAGGAATATAACCCCACTCAGAAGGCCTGGAAGTATCTCAATACATTAGCTGAGGGCAATGCTGTGCCGGAGGACATCTTGCCCTTTGAAGGCGAACTAGAAGAAGAAGAATATTACTACCCGAGCTTGCCATTCGCGGCACTTTTTTCTTGTCTCAAGGCCAAAGGATTGAAGGTTACCTGCTTATTATGCTACTGCTCAGAAGGAGACAACATACCTGATGCTTTCCATTTGGCCGAGGCAGCATGCAAACTTCTGCAATTAAATCCCAGTAATTTCCATGGCAACAATGATAAATGGGTTATTCCATGTTCTTGGAAGACTCTGTATGGACCACCGGCCGATATGTCCCTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC2

Protein Analysis

282

Amino Acids

30.81

Weight (kDa)

4.84

Isoelectric Point (pI)

49.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAC2 PF09754 18 - 248 7.9e-25 PAC2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 674
AccII CGCG 1 cut(s) 623
AciI CCGC 2 cut(s) 95, 623
AclWI GGATC 1 cut(s) 49
AcoI YGGCCR 2 cut(s) 720, 825
AcsI RAATTY 2 cut(s) 5, 299
AfiI CCNNNNNNNGG 2 cut(s) 170, 575
AgsI TTSAA 4 cut(s) 224, 299, 575, 658
AjnI CCWGG 1 cut(s) 512
AluBI AGCT 8 cut(s) 65, 91, 208, 227, 366, 469, 536, 612
AluI AGCT 8 cut(s) 65, 91, 208, 227, 366, 469, 536, 612
Alw21I GWGCWC 1 cut(s) 360
Alw26I GTCTC 3 cut(s) 258, 644, 687
AlwI GGATC 1 cut(s) 49
Ama87I CYCGRG 2 cut(s) 361, 607
AoxI GGCC 5 cut(s) 188, 510, 645, 720, 825
ApeKI GCWGC 4 cut(s) 88, 329, 484, 728
ApoI RAATTY 2 cut(s) 5, 299
ArsI GACNNNNNNTTYG 2 cut(s) 137, 169
AspS9I GGNCC 4 cut(s) 100, 188, 270, 818
AvaI CYCGRG 2 cut(s) 361, 607
AvaII GGWCC 3 cut(s) 100, 270, 818
BbsI GAAGAC 1 cut(s) 812
Bbv12I GWGCWC 1 cut(s) 360
BbvCI CCTCAGC 1 cut(s) 537
BbvI GCAGC 4 cut(s) 100, 316, 471, 740
BccI CCATC 1 cut(s) 437
BciT130I CCWGG 1 cut(s) 514
BcoDI GTCTC 3 cut(s) 258, 644, 687
BfaI CTAG 4 cut(s) 107, 333, 429, 584
BfmI CTRYAG 1 cut(s) 485
BfuAI ACCTGC 1 cut(s) 674
BisI GCNGC 5 cut(s) 89, 330, 485, 624, 729
BlsI GCNGC 5 cut(s) 90, 331, 486, 625, 730
Bme1390I CCNGG 1 cut(s) 514
Bme18I GGWCC 3 cut(s) 100, 270, 818
BmeT110I CYCGRG 2 cut(s) 361, 607
BmgT120I GGNCC 4 cut(s) 100, 188, 270, 818
BmiI GGNNCC 2 cut(s) 162, 272
BmrFI CCNGG 1 cut(s) 514
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 4 cut(s) 122, 169, 405, 697
BmuI ACTGGG 1 cut(s) 750
BpiI GAAGAC 1 cut(s) 812
Bpu10I CCTNAGC 1 cut(s) 537
BpuEI CTTGAG 1 cut(s) 626
BsaJI CCNNGG 3 cut(s) 34, 723, 766
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 575
Bse118I RCCGGY 1 cut(s) 823
Bse1I ACTGG 1 cut(s) 756
Bse3DI GCAATG 3 cut(s) 181, 255, 550
BseBI CCWGG 1 cut(s) 514
BseDI CCNNGG 3 cut(s) 34, 723, 766
BseGI GGATG 1 cut(s) 297
BseLI CCNNNNNNNGG 2 cut(s) 170, 575
BseMI GCAATG 3 cut(s) 181, 255, 550
BseMII CTCAG 3 cut(s) 518, 528, 699
BseNI ACTGG 1 cut(s) 756
BseX3I CGGCCG 1 cut(s) 825
BseXI GCAGC 4 cut(s) 100, 316, 471, 740
Bsh1236I CGCG 1 cut(s) 623
Bsh1285I CGRYCG 1 cut(s) 828
BshFI GGCC 5 cut(s) 190, 512, 647, 722, 827
BsiEI CGRYCG 1 cut(s) 828
BsiHKAI GWGCWC 1 cut(s) 360
BsiHKCI CYCGRG 2 cut(s) 361, 607
BsiSI CCGG 2 cut(s) 554, 824
BslFI GGGAC 2 cut(s) 256, 820
BslI CCNNNNNNNGG 2 cut(s) 170, 575
BsmAI GTCTC 3 cut(s) 258, 644, 687
BsmFI GGGAC 2 cut(s) 256, 820
BsnI GGCC 5 cut(s) 190, 512, 647, 722, 827
BsoBI CYCGRG 2 cut(s) 361, 607
Bsp1286I GDGCHC 1 cut(s) 360
Bsp143I GATC 1 cut(s) 54
Bsp19I CCATGG 2 cut(s) 34, 766
BspACI CCGC 2 cut(s) 95, 623
BspANI GGCC 5 cut(s) 190, 512, 647, 722, 827
BspCNI CTCAG 3 cut(s) 517, 529, 698
BspFNI CGCG 1 cut(s) 623
BspLI GGNNCC 2 cut(s) 162, 272
BspMAI CTGCAG 1 cut(s) 489
BspMI ACCTGC 1 cut(s) 674
BspPI GGATC 1 cut(s) 49
BspQI GCTCTTC 1 cut(s) 49
BsrDI GCAATG 3 cut(s) 181, 255, 550
BsrFI RCCGGY 1 cut(s) 823
BsrI ACTGG 1 cut(s) 756
BssAI RCCGGY 1 cut(s) 823
BssECI CCNNGG 3 cut(s) 34, 723, 766
BssMI GATC 1 cut(s) 54
BssT1I CCWWGG 2 cut(s) 34, 766
Bst2UI CCWGG 1 cut(s) 514
Bst6I CTCTTC 1 cut(s) 49
BstC8I GCNNGC 5 cut(s) 63, 93, 210, 614, 733
BstDEI CTNAG 3 cut(s) 504, 537, 685
BstDSI CCRYGG 2 cut(s) 34, 766
BstEII GGTNACC 1 cut(s) 661
BstF5I GGATG 1 cut(s) 297
BstFNI CGCG 1 cut(s) 623
BstKTI GATC 1 cut(s) 57
BstMAI GTCTC 3 cut(s) 258, 644, 687
BstMBI GATC 1 cut(s) 54
BstMCI CGRYCG 1 cut(s) 828
BstMWI GCNNNNNNNGC 2 cut(s) 675, 728
BstNI CCWGG 1 cut(s) 514
BstNSI RCATGY 1 cut(s) 735
BstPI GGTNACC 1 cut(s) 661
BstSCI CCNGG 1 cut(s) 512
BstSFI CTRYAG 1 cut(s) 485
BstUI CGCG 1 cut(s) 623
BstV1I GCAGC 4 cut(s) 100, 316, 471, 740
BstV2I GAAGAC 1 cut(s) 812
BstXI CCANNNNNNTGG 1 cut(s) 801
BstZI CGGCCG 1 cut(s) 825
BsuRI GGCC 5 cut(s) 190, 512, 647, 722, 827
BtgI CCRYGG 2 cut(s) 34, 766
BtsCI GGATG 1 cut(s) 297
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 5 cut(s) 63, 93, 210, 614, 733
Cfr10I RCCGGY 1 cut(s) 823
Cfr13I GGNCC 4 cut(s) 100, 188, 270, 818
CviAII CATG 4 cut(s) 35, 732, 767, 795
DdeI CTNAG 3 cut(s) 504, 537, 685
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
EaeI YGGCCR 2 cut(s) 720, 825
EagI CGGCCG 1 cut(s) 825
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
EclXI CGGCCG 1 cut(s) 825
Eco130I CCWWGG 2 cut(s) 34, 766
Eco147I AGGCCT 1 cut(s) 512
Eco47I GGWCC 3 cut(s) 100, 270, 818
Eco52I CGGCCG 1 cut(s) 825
Eco88I CYCGRG 2 cut(s) 361, 607
Eco91I GGTNACC 1 cut(s) 661
EcoO109I RGGNCCY 1 cut(s) 270
EcoO65I GGTNACC 1 cut(s) 661
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 512
EcoT14I CCWWGG 2 cut(s) 34, 766
EcoT22I ATGCAT 1 cut(s) 420
ErhI CCWWGG 2 cut(s) 34, 766
FaeI CATG 4 cut(s) 38, 735, 770, 798
FaqI GGGAC 2 cut(s) 256, 820
FatI CATG 4 cut(s) 34, 731, 766, 794
Fnu4HI GCNGC 5 cut(s) 89, 330, 485, 624, 729
FokI GGATG 1 cut(s) 304
Fsp4HI GCNGC 5 cut(s) 89, 330, 485, 624, 729
FspBI CTAG 4 cut(s) 107, 333, 429, 584
GluI GCNGC 5 cut(s) 89, 330, 485, 624, 729
HaeIII GGCC 5 cut(s) 190, 512, 647, 722, 827
HapII CCGG 2 cut(s) 554, 824
Hin1II CATG 4 cut(s) 38, 735, 770, 798
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 2 cut(s) 233, 808
HpaII CCGG 2 cut(s) 554, 824
Hpy166II GTNNAC 2 cut(s) 100, 464
Hpy188I TCNGA 4 cut(s) 54, 442, 507, 688
Hpy188III TCNNGA 2 cut(s) 221, 361
Hpy8I GTNNAC 2 cut(s) 100, 464
HpyAV CCTTC 6 cut(s) 113, 282, 502, 569, 652, 683
HpyCH4V TGCA 7 cut(s) 113, 248, 412, 418, 487, 735, 745
HpyF10VI GCNNNNNNNGC 2 cut(s) 675, 728
HpyF3I CTNAG 3 cut(s) 504, 537, 685
Hsp92II CATG 4 cut(s) 38, 735, 770, 798
Kzo9I GATC 1 cut(s) 54
LguI GCTCTTC 1 cut(s) 49
LmnI GCTCC 1 cut(s) 205
Lsp1109I GCAGC 4 cut(s) 100, 316, 471, 740
LweI GCATC 4 cut(s) 122, 169, 405, 697
MaeI CTAG 4 cut(s) 107, 333, 429, 584
MaeIII GTNAC 1 cut(s) 661
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MboII GAAGA 7 cut(s) 36, 231, 355, 599, 602, 605, 817
MfeI CAATTG 1 cut(s) 72
MhlI GDGCHC 1 cut(s) 360
MluCI AATT 6 cut(s) 5, 72, 299, 436, 746, 760
MlyI GAGTC 1 cut(s) 802
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 5 cut(s) 228, 372, 532, 550, 718
Mph1103I ATGCAT 1 cut(s) 420
MseI TTAA 1 cut(s) 749
MslI CAYNNNNRTG 1 cut(s) 33
MspI CCGG 2 cut(s) 554, 824
MspR9I CCNGG 1 cut(s) 514
MunI CAATTG 1 cut(s) 72
MvaI CCWGG 1 cut(s) 514
MvnI CGCG 1 cut(s) 623
MwoI GCNNNNNNNGC 2 cut(s) 675, 728
NcoI CCATGG 2 cut(s) 34, 766
NdeII GATC 1 cut(s) 54
NlaIII CATG 4 cut(s) 38, 735, 770, 798
NlaIV GGNNCC 2 cut(s) 162, 272
NmeAIII GCCGAG 1 cut(s) 748
NsiI ATGCAT 1 cut(s) 420
NspI RCATGY 1 cut(s) 735
PaeI GCATGC 1 cut(s) 735
PaeR7I CTCGAG 1 cut(s) 361
PceI AGGCCT 1 cut(s) 512
PciSI GCTCTTC 1 cut(s) 49
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 5 cut(s) 90, 331, 486, 625, 730
PleI GAGTC 1 cut(s) 802
PpsI GAGTC 1 cut(s) 802
PpuMI RGGWCCY 1 cut(s) 270
Psp5II RGGWCCY 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 512
PspEI GGTNACC 1 cut(s) 661
PspGI CCWGG 1 cut(s) 512
PspN4I GGNNCC 2 cut(s) 162, 272
PspPI GGNCC 4 cut(s) 100, 188, 270, 818
PspPPI RGGWCCY 1 cut(s) 270
PstI CTGCAG 1 cut(s) 489
RseI CAYNNNNRTG 1 cut(s) 33
SapI GCTCTTC 1 cut(s) 49
SaqAI TTAA 1 cut(s) 749
SatI GCNGC 5 cut(s) 89, 330, 485, 624, 729
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 4 cut(s) 100, 188, 270, 818
SchI GAGTC 1 cut(s) 802
ScrFI CCNGG 1 cut(s) 514
SduI GDGCHC 1 cut(s) 360
SfaNI GCATC 4 cut(s) 122, 169, 405, 697
SfcI CTRYAG 1 cut(s) 485
Sfr274I CTCGAG 1 cut(s) 361
SinI GGWCC 3 cut(s) 100, 270, 818
SlaI CTCGAG 1 cut(s) 361
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 2 cut(s) 361, 641
SmoI CTYRAG 2 cut(s) 361, 641
SphI GCATGC 1 cut(s) 735
Sse9I AATT 6 cut(s) 5, 72, 299, 436, 746, 760
SseBI AGGCCT 1 cut(s) 512
SsiI CCGC 2 cut(s) 95, 623
SspI AATATT 1 cut(s) 599
SspMI CTAG 4 cut(s) 107, 333, 429, 584
StuI AGGCCT 1 cut(s) 512
StyD4I CCNGG 1 cut(s) 512
StyI CCWWGG 2 cut(s) 34, 766
TaqI TCGA 1 cut(s) 362
TasI AATT 6 cut(s) 5, 72, 299, 436, 746, 760
TauI GCSGC 1 cut(s) 626
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 1 cut(s) 749
Tru9I TTAA 1 cut(s) 749
TseI GCWGC 4 cut(s) 88, 329, 484, 728
TspDTI ATGAA 2 cut(s) 134, 225
VpaK11BI GGWCC 3 cut(s) 100, 270, 818
XapI RAATTY 2 cut(s) 5, 299
XceI RCATGY 1 cut(s) 735
XhoI CTCGAG 1 cut(s) 361
XspI CTAG 4 cut(s) 107, 333, 429, 584
Zsp2I ATGCAT 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.