Rw6G009680

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002770
Physical Location & Seq
Forward (+)
16253723 .. 16258540
4818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G009680.1

Sequence Viewer

Length: 846 bp
ATGGAATTCATTCCCGAAGAAGGCACACACAACCACGCCGACGCCTCCACTCTAATCCTGCCGGCGTTGTCGATCGGAAATGTGGGCCAGCTCGCCGTGGACCTCTTAGTCGCGTCGACGAAGGCGGAGAGAGTCGGGTTTCTGGATAGTCCGTATGTCCTTCCATGTGTTGGCAACGATGCATATGGGCCTGTTCCTCAAGGCCACCTTGCTCTTCCTCTTGAAGCTTATGAATCGGCTTCTAATGGAATGGCTTTAATCCAGCAAAGGTCACCTGTTGTTAAGGGTAGGATGATTGAGTTTGCTAAGACGTTGGCTGATTATGTTGCTGCGAGTGGGAAGAAGCATGTGATTGTGCTTTCGAGCTTGGACTTTGGGAGGTGGCAGAGAATTGATATGTCAAGTGGTTCCCAGATGCATTACCTATCTAGTAGCAATGCCGATGGAACAGATGACTACTGCGAAAAGCTTGGGTGGAAAAGACTTCAGGATTATAACCCCAATCAGAGGTCATGGAAGTATCTTAACACATTAGCTGATGGCAATGCCATGCCAGAGGACAGCTTACCTTTTGAAGGTGAATTGGAAGAAGAAGAATACTACTACCCAAGCTTGCCTTTTGCAGCTCTCTTTTCTTCCTTCAAGGCAAAAGGATTGAAGGTCACCTGTATATTATGCTACTGCTCAGAAGGAGACAACACACCTGATGCTTTCCATTTAGCTGAAGCAGCATGCAAACTTCTGGGTGTGAGTCCCAGTAATTTCCATGGCAACACTGATAATTGGCTTATTCCAATTTCTTGGAAGACTGTGTATGGACCACCCGCAGATATGTCTCTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC2

Protein Analysis

281

Amino Acids

30.8

Weight (kDa)

4.83

Isoelectric Point (pI)

50.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAC2 PF09754 18 - 250 3.4e-25 PAC2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
AasI GACNNNNNNGTC 1 cut(s) 107
AccB7I CCANNNNNTGG 1 cut(s) 170
AccI GTMKAC 1 cut(s) 116
AccII CGCG 1 cut(s) 113
AciI CCGC 2 cut(s) 125, 825
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 2 cut(s) 470, 744
AcyI GRCGYC 1 cut(s) 42
AfiI CCNNNNNNNGG 4 cut(s) 20, 170, 507, 575
AgsI TTSAA 4 cut(s) 224, 575, 643, 658
AluBI AGCT 9 cut(s) 91, 227, 366, 469, 536, 564, 612, 626, 722
AluI AGCT 9 cut(s) 91, 227, 366, 469, 536, 564, 612, 626, 722
Alw26I GTCTC 2 cut(s) 687, 840
AoxI GGCC 3 cut(s) 85, 188, 202
ApeKI GCWGC 3 cut(s) 329, 623, 728
ApoI RAATTY 1 cut(s) 5
Asp700I GAANNNNTTC 1 cut(s) 9
AspS9I GGNCC 4 cut(s) 85, 100, 188, 818
AsuHPI GGTGA 3 cut(s) 264, 590, 655
AvaII GGWCC 2 cut(s) 100, 818
BbsI GAAGAC 1 cut(s) 812
BbvI GCAGC 3 cut(s) 316, 635, 740
BccI CCATC 2 cut(s) 437, 533
BceAI ACGGC 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 687, 840
BfaI CTAG 1 cut(s) 429
BisI GCNGC 3 cut(s) 330, 624, 729
BlsI GCNGC 3 cut(s) 331, 625, 730
Bme18I GGWCC 2 cut(s) 100, 818
BmgT120I GGNCC 4 cut(s) 85, 100, 188, 818
BmiI GGNNCC 1 cut(s) 409
BmrI ACTGGG 1 cut(s) 750
BmsI GCATC 3 cut(s) 169, 405, 697
BmuI ACTGGG 1 cut(s) 750
BpiI GAAGAC 1 cut(s) 812
BpuEI CTTGAG 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 2 cut(s) 96, 766
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 170, 507, 575
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 756
Bse3DI GCAATG 2 cut(s) 442, 550
BseDI CCNNGG 2 cut(s) 96, 766
BseGI GGATG 1 cut(s) 297
BseLI CCNNNNNNNGG 4 cut(s) 20, 170, 507, 575
BseMI GCAATG 2 cut(s) 442, 550
BseMII CTCAG 1 cut(s) 699
BseNI ACTGG 1 cut(s) 756
BseXI GCAGC 3 cut(s) 316, 635, 740
Bsh1236I CGCG 1 cut(s) 113
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 3 cut(s) 87, 190, 204
BsiEI CGRYCG 1 cut(s) 75
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 1 cut(s) 738
BslI CCNNNNNNNGG 4 cut(s) 20, 170, 507, 575
BsmAI GTCTC 2 cut(s) 687, 840
BsmFI GGGAC 1 cut(s) 738
BsnI GGCC 3 cut(s) 87, 190, 204
Bsp143I GATC 1 cut(s) 72
Bsp19I CCATGG 1 cut(s) 766
BspACI CCGC 2 cut(s) 125, 825
BspANI GGCC 3 cut(s) 87, 190, 204
BspCNI CTCAG 1 cut(s) 698
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 409
BspQI GCTCTTC 1 cut(s) 219
BsrDI GCAATG 2 cut(s) 442, 550
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 1 cut(s) 756
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 2 cut(s) 96, 766
BssMI GATC 1 cut(s) 72
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 766
Bst4CI ACNGT 1 cut(s) 811
Bst6I CTCTTC 1 cut(s) 219
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 5 cut(s) 63, 89, 93, 614, 733
BstDEI CTNAG 3 cut(s) 106, 306, 685
BstDSI CCRYGG 2 cut(s) 96, 766
BstEII GGTNACC 2 cut(s) 270, 661
BstENI CCTNNNNNAGG 1 cut(s) 573
BstF5I GGATG 1 cut(s) 297
BstFNI CGCG 1 cut(s) 113
BstKTI GATC 1 cut(s) 75
BstMAI GTCTC 2 cut(s) 687, 840
BstMBI GATC 1 cut(s) 72
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 1 cut(s) 728
BstNSI RCATGY 2 cut(s) 350, 735
BstPI GGTNACC 2 cut(s) 270, 661
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 3 cut(s) 316, 635, 740
BstV2I GAAGAC 1 cut(s) 812
BstXI CCANNNNNNTGG 1 cut(s) 801
BsuRI GGCC 3 cut(s) 87, 190, 204
BtgI CCRYGG 2 cut(s) 96, 766
BtsCI GGATG 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 774
Cac8I GCNNGC 5 cut(s) 63, 89, 93, 614, 733
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 4 cut(s) 85, 100, 188, 818
CseI GACGC 2 cut(s) 50, 102
CviAII CATG 6 cut(s) 165, 347, 513, 550, 732, 767
DdeI CTNAG 3 cut(s) 106, 306, 685
DpnI GATC 1 cut(s) 74
DpnII GATC 1 cut(s) 72
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
EciI GGCGGA 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 766
Eco47I GGWCC 2 cut(s) 100, 818
Eco57I CTGAAG 2 cut(s) 470, 744
Eco91I GGTNACC 2 cut(s) 270, 661
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoO65I GGTNACC 2 cut(s) 270, 661
EcoRI GAATTC 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 766
EcoT22I ATGCAT 2 cut(s) 184, 420
ErhI CCWWGG 1 cut(s) 766
FaeI CATG 6 cut(s) 168, 350, 516, 553, 735, 770
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 601, 633
FaqI GGGAC 1 cut(s) 738
FatI CATG 6 cut(s) 164, 346, 512, 549, 731, 766
FauI CCCGC 1 cut(s) 832
FauNDI CATATG 1 cut(s) 184
FblI GTMKAC 1 cut(s) 116
Fnu4HI GCNGC 3 cut(s) 330, 624, 729
FokI GGATG 1 cut(s) 304
Fsp4HI GCNGC 3 cut(s) 330, 624, 729
FspBI CTAG 1 cut(s) 429
GluI GCNGC 3 cut(s) 330, 624, 729
HaeIII GGCC 3 cut(s) 87, 190, 204
HapII CCGG 1 cut(s) 62
HgaI GACGC 2 cut(s) 50, 102
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 6 cut(s) 168, 350, 516, 553, 735, 770
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HindIII AAGCTT 3 cut(s) 225, 467, 610
HinfI GANTC 3 cut(s) 132, 233, 751
HpaII CCGG 1 cut(s) 62
HphI GGTGA 3 cut(s) 264, 590, 655
Hpy166II GTNNAC 2 cut(s) 100, 117
Hpy188I TCNGA 3 cut(s) 77, 507, 688
Hpy188III TCNNGA 4 cut(s) 14, 143, 221, 488
Hpy8I GTNNAC 2 cut(s) 100, 117
Hpy99I CGWCG 3 cut(s) 44, 118, 121
HpyAV CCTTC 7 cut(s) 14, 115, 170, 569, 649, 652, 683
HpyCH4III ACNGT 1 cut(s) 811
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 4 cut(s) 182, 418, 623, 735
HpyF10VI GCNNNNNNNGC 1 cut(s) 728
HpyF3I CTNAG 3 cut(s) 106, 306, 685
HpySE526I ACGT 1 cut(s) 311
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 6 cut(s) 168, 350, 516, 553, 735, 770
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Kzo9I GATC 1 cut(s) 72
LguI GCTCTTC 1 cut(s) 219
Lsp1109I GCAGC 3 cut(s) 316, 635, 740
LweI GCATC 3 cut(s) 169, 405, 697
MaeI CTAG 1 cut(s) 429
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 2 cut(s) 270, 661
MalI GATC 1 cut(s) 74
MboI GATC 1 cut(s) 72
MboII GAAGA 8 cut(s) 29, 206, 352, 599, 602, 605, 627, 817
MluCI AATT 6 cut(s) 5, 390, 581, 760, 781, 795
MlyI GAGTC 2 cut(s) 141, 760
MnlI CCTC 7 cut(s) 55, 113, 207, 228, 372, 501, 550
Mph1103I ATGCAT 2 cut(s) 184, 420
MroNI GCCGGC 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 4 cut(s) 257, 282, 525, 844
MspI CCGG 1 cut(s) 62
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 728
NaeI GCCGGC 1 cut(s) 63
NcoI CCATGG 1 cut(s) 766
NdeI CATATG 1 cut(s) 184
NdeII GATC 1 cut(s) 72
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 6 cut(s) 168, 350, 516, 553, 735, 770
NlaIV GGNNCC 1 cut(s) 409
NmuCI GTSAC 2 cut(s) 270, 661
NsiI ATGCAT 2 cut(s) 184, 420
NspI RCATGY 2 cut(s) 350, 735
PaeI GCATGC 1 cut(s) 735
PciSI GCTCTTC 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 1 cut(s) 233
PflMI CCANNNNNTGG 1 cut(s) 170
PkrI GCNGC 3 cut(s) 331, 625, 730
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 2 cut(s) 140, 759
PpsI GAGTC 2 cut(s) 140, 759
PsiI TTATAA 1 cut(s) 495
PspEI GGTNACC 2 cut(s) 270, 661
PspN4I GGNNCC 1 cut(s) 409
PspPI GGNCC 4 cut(s) 85, 100, 188, 818
PvuI CGATCG 1 cut(s) 75
SalI GTCGAC 1 cut(s) 115
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 4 cut(s) 257, 282, 525, 844
SatI GCNGC 3 cut(s) 330, 624, 729
Sau3AI GATC 1 cut(s) 72
Sau96I GGNCC 4 cut(s) 85, 100, 188, 818
SchI GAGTC 2 cut(s) 141, 760
SfaNI GCATC 3 cut(s) 169, 405, 697
SgrDI CGTCGACG 1 cut(s) 115
SinI GGWCC 2 cut(s) 100, 818
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
SphI GCATGC 1 cut(s) 735
Sse9I AATT 6 cut(s) 5, 390, 581, 760, 781, 795
SsiI CCGC 2 cut(s) 125, 825
SspMI CTAG 1 cut(s) 429
StyI CCWWGG 1 cut(s) 766
TaaI ACNGT 1 cut(s) 811
TaiI ACGT 1 cut(s) 314
TaqI TCGA 3 cut(s) 71, 116, 362
TasI AATT 6 cut(s) 5, 390, 581, 760, 781, 795
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 4 cut(s) 257, 282, 525, 844
Tru9I TTAA 4 cut(s) 257, 282, 525, 844
TscAI CASTG 1 cut(s) 781
TseFI GTSAC 2 cut(s) 270, 661
TseI GCWGC 3 cut(s) 329, 623, 728
Tsp45I GTSAC 2 cut(s) 270, 661
TspDTI ATGAA 1 cut(s) 246
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 1 cut(s) 781
Van91I CCANNNNNTGG 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 100, 818
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 1 cut(s) 5
XceI RCATGY 2 cut(s) 350, 735
XmiI GTMKAC 1 cut(s) 116
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 1 cut(s) 429
Zsp2I ATGCAT 2 cut(s) 184, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.