RLG00000014474

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002770
Physical Location & Seq
Reverse (-)
53606362 .. 53608489
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014474

Sequence Viewer

Length: 663 bp
ATGGAACTAATTCCCGAAGAAGGCACACACAACCACGCCGACTCCTCCACTCTAATCCTGCCGGCGCTGTCGATCGGAAATGTGGGCCAGCTCGCCGTGGATCTCTTAGTCACGTCGACGAAGGCTGAGAGAGTCGGGTTTCTGGATAGTCCGTATGTCCTTCCATGTGTTGGCAACGATGCATATGGCCCTGTTCCTCAAGGCCACCTTGCTCTTCCTCTTGAAGCTTATGAATCGTCTTCTAATGGAATGGCTTTAATCCAGCAAAGGTCACCTGTTGTTAAGTTTGAAATGGTAATTCTTACAGGGCAGAGAATTGATATGTCAAGTGGTTCCCAGATGCATTACCTGTCTAGCAGCAATGCCGATGGAAAAGATGATTACTGCGAACAGCTTGGGTCATGGAAGTATCTTAACACATTAGCTGATGGCAATGCCATGCCAGAGGACAGCTTACCTTTTGAAGGTGAATTGGAAGAAGAAGAATACTACTACCCAAGCTTGCCTTTTTCAGCTCTCTTTTCTTCCTTCAAGGCAAAAGGATTGAAGGTCACCTGTTTATTATGCTACTGCTCAGAAGGAGACAACACACCTGATGCTTTCCATTTGGCTGAAGCAGCATGCAAACTTCTGGGTGTGAGTCCCAGTAATTTCCATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC2

Protein Analysis

221

Amino Acids

23.77

Weight (kDa)

4.44

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAC2 PF09754 18 - 213 6.8e-17 PAC2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 170
AccI GTMKAC 1 cut(s) 116
AclWI GGATC 1 cut(s) 108
AcuI CTGAAG 1 cut(s) 633
AfiI CCNNNNNNNGG 3 cut(s) 20, 170, 464
AgsI TTSAA 5 cut(s) 224, 290, 464, 532, 547
AjiI CACGTC 1 cut(s) 114
AluBI AGCT 7 cut(s) 91, 227, 394, 425, 453, 501, 515
AluI AGCT 7 cut(s) 91, 227, 394, 425, 453, 501, 515
Alw26I GTCTC 1 cut(s) 576
AlwI GGATC 1 cut(s) 108
AoxI GGCC 3 cut(s) 85, 187, 202
ApeKI GCWGC 2 cut(s) 357, 617
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 2 cut(s) 85, 188
AsuHPI GGTGA 3 cut(s) 264, 479, 544
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 369, 629
BccI CCATC 2 cut(s) 362, 422
BceAI ACGGC 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 576
BfaI CTAG 1 cut(s) 354
BfoI RGCGCY 1 cut(s) 68
BisI GCNGC 2 cut(s) 358, 618
BlsI GCNGC 2 cut(s) 359, 619
BmgBI CACGTC 1 cut(s) 114
BmgT120I GGNCC 2 cut(s) 85, 188
BmiI GGNNCC 1 cut(s) 334
BmrI ACTGGG 1 cut(s) 639
BmsI GCATC 3 cut(s) 169, 330, 586
BmuI ACTGGG 1 cut(s) 639
BpiI GAAGAC 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 96, 655
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 170, 464
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 645
Bse3DI GCAATG 2 cut(s) 367, 439
BseDI CCNNGG 2 cut(s) 96, 655
BseLI CCNNNNNNNGG 3 cut(s) 20, 170, 464
BseMI GCAATG 2 cut(s) 367, 439
BseMII CTCAG 2 cut(s) 117, 588
BseNI ACTGG 1 cut(s) 645
BseRI GAGGAG 1 cut(s) 34
BseXI GCAGC 2 cut(s) 369, 629
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 3 cut(s) 87, 189, 204
BsiEI CGRYCG 1 cut(s) 75
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 1 cut(s) 627
BslI CCNNNNNNNGG 3 cut(s) 20, 170, 464
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 1 cut(s) 627
BsnI GGCC 3 cut(s) 87, 189, 204
Bsp143I GATC 2 cut(s) 72, 100
Bsp19I CCATGG 1 cut(s) 655
BspANI GGCC 3 cut(s) 87, 189, 204
BspCNI CTCAG 2 cut(s) 118, 587
BspLI GGNNCC 1 cut(s) 334
BspPI GGATC 1 cut(s) 108
BspQI GCTCTTC 1 cut(s) 219
BsrDI GCAATG 2 cut(s) 367, 439
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 1 cut(s) 645
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 2 cut(s) 96, 655
BssMI GATC 2 cut(s) 72, 100
BssT1I CCWWGG 1 cut(s) 655
Bst6I CTCTTC 1 cut(s) 219
BstC8I GCNNGC 5 cut(s) 63, 89, 93, 503, 622
BstDEI CTNAG 3 cut(s) 106, 126, 574
BstDSI CCRYGG 2 cut(s) 96, 655
BstEII GGTNACC 2 cut(s) 270, 550
BstENI CCTNNNNNAGG 1 cut(s) 462
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 2 cut(s) 75, 103
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 2 cut(s) 72, 100
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 1 cut(s) 617
BstNSI RCATGY 1 cut(s) 624
BstPI GGTNACC 2 cut(s) 270, 550
BstV1I GCAGC 2 cut(s) 369, 629
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 3 cut(s) 87, 189, 204
BtgI CCRYGG 2 cut(s) 96, 655
BtrI CACGTC 1 cut(s) 114
Cac8I GCNNGC 5 cut(s) 63, 89, 93, 503, 622
CfoI GCGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 2 cut(s) 85, 188
CviAII CATG 5 cut(s) 165, 402, 439, 621, 656
DdeI CTNAG 3 cut(s) 106, 126, 574
DpnI GATC 2 cut(s) 74, 102
DpnII GATC 2 cut(s) 72, 100
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 655
Eco57I CTGAAG 1 cut(s) 633
Eco91I GGTNACC 2 cut(s) 270, 550
EcoNI CCTNNNNNAGG 1 cut(s) 462
EcoO65I GGTNACC 2 cut(s) 270, 550
EcoT14I CCWWGG 1 cut(s) 655
EcoT22I ATGCAT 2 cut(s) 184, 345
ErhI CCWWGG 1 cut(s) 655
FaeI CATG 5 cut(s) 168, 405, 442, 624, 659
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 490, 522
FaqI GGGAC 1 cut(s) 627
FatI CATG 5 cut(s) 164, 401, 438, 620, 655
FauNDI CATATG 1 cut(s) 184
FblI GTMKAC 1 cut(s) 116
Fnu4HI GCNGC 2 cut(s) 358, 618
Fsp4HI GCNGC 2 cut(s) 358, 618
FspBI CTAG 1 cut(s) 354
GlaI GCGC 1 cut(s) 66
GluI GCNGC 2 cut(s) 358, 618
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 3 cut(s) 87, 189, 204
HapII CCGG 1 cut(s) 62
HhaI GCGC 1 cut(s) 67
Hin1II CATG 5 cut(s) 168, 405, 442, 624, 659
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HindIII AAGCTT 2 cut(s) 225, 499
HinfI GANTC 4 cut(s) 41, 132, 233, 640
HpaII CCGG 1 cut(s) 62
HphI GGTGA 3 cut(s) 264, 479, 544
Hpy166II GTNNAC 1 cut(s) 117
Hpy188I TCNGA 2 cut(s) 77, 577
Hpy188III TCNNGA 3 cut(s) 14, 143, 221
Hpy8I GTNNAC 1 cut(s) 117
Hpy99I CGWCG 2 cut(s) 118, 121
HpyAV CCTTC 7 cut(s) 14, 115, 170, 458, 538, 541, 572
HpyCH4IV ACGT 1 cut(s) 113
HpyCH4V TGCA 3 cut(s) 182, 343, 624
HpyF10VI GCNNNNNNNGC 1 cut(s) 617
HpyF3I CTNAG 3 cut(s) 106, 126, 574
HpySE526I ACGT 1 cut(s) 113
Hsp92II CATG 5 cut(s) 168, 405, 442, 624, 659
HspAI GCGC 1 cut(s) 65
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Kzo9I GATC 2 cut(s) 72, 100
LguI GCTCTTC 1 cut(s) 219
Lsp1109I GCAGC 2 cut(s) 369, 629
LweI GCATC 3 cut(s) 169, 330, 586
MaeI CTAG 1 cut(s) 354
MaeII ACGT 1 cut(s) 113
MaeIII GTNAC 3 cut(s) 109, 270, 550
MalI GATC 2 cut(s) 74, 102
MboI GATC 2 cut(s) 72, 100
MboII GAAGA 7 cut(s) 29, 206, 231, 488, 491, 494, 516
MflI RGATCY 1 cut(s) 100
MluCI AATT 5 cut(s) 9, 297, 315, 470, 649
MlyI GAGTC 3 cut(s) 35, 141, 649
MnlI CCTC 4 cut(s) 55, 207, 228, 439
Mph1103I ATGCAT 2 cut(s) 184, 345
MroNI GCCGGC 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 3 cut(s) 257, 282, 414
MspI CCGG 1 cut(s) 62
MwoI GCNNNNNNNGC 1 cut(s) 617
NaeI GCCGGC 1 cut(s) 63
NcoI CCATGG 1 cut(s) 655
NdeI CATATG 1 cut(s) 184
NdeII GATC 2 cut(s) 72, 100
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 5 cut(s) 168, 405, 442, 624, 659
NlaIV GGNNCC 1 cut(s) 334
NmuCI GTSAC 3 cut(s) 109, 270, 550
NsiI ATGCAT 2 cut(s) 184, 345
NspI RCATGY 1 cut(s) 624
PaeI GCATGC 1 cut(s) 624
PciSI GCTCTTC 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 1 cut(s) 233
PflMI CCANNNNNTGG 1 cut(s) 170
PkrI GCNGC 2 cut(s) 359, 619
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 3 cut(s) 35, 140, 648
PpsI GAGTC 3 cut(s) 35, 140, 648
PspEI GGTNACC 2 cut(s) 270, 550
PspN4I GGNNCC 1 cut(s) 334
PspPI GGNCC 2 cut(s) 85, 188
PsuI RGATCY 1 cut(s) 100
PvuI CGATCG 1 cut(s) 75
SalI GTCGAC 1 cut(s) 115
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 3 cut(s) 257, 282, 414
SatI GCNGC 2 cut(s) 358, 618
Sau3AI GATC 2 cut(s) 72, 100
Sau96I GGNCC 2 cut(s) 85, 188
SchI GAGTC 3 cut(s) 35, 141, 649
SfaNI GCATC 3 cut(s) 169, 330, 586
SgrDI CGTCGACG 1 cut(s) 115
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
SphI GCATGC 1 cut(s) 624
Sse9I AATT 5 cut(s) 9, 297, 315, 470, 649
SspMI CTAG 1 cut(s) 354
StyI CCWWGG 1 cut(s) 655
TaiI ACGT 1 cut(s) 116
TaqI TCGA 2 cut(s) 71, 116
TasI AATT 5 cut(s) 9, 297, 315, 470, 649
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 3 cut(s) 257, 282, 414
Tru9I TTAA 3 cut(s) 257, 282, 414
TseFI GTSAC 3 cut(s) 109, 270, 550
TseI GCWGC 2 cut(s) 357, 617
Tsp45I GTSAC 3 cut(s) 109, 270, 550
TspDTI ATGAA 1 cut(s) 246
TspGWI ACGGA 1 cut(s) 141
Van91I CCANNNNNTGG 1 cut(s) 170
XagI CCTNNNNNAGG 1 cut(s) 462
XceI RCATGY 1 cut(s) 624
XmiI GTMKAC 1 cut(s) 116
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 1 cut(s) 354
Zsp2I ATGCAT 2 cut(s) 184, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.