RLG00000014468

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002770
Physical Location & Seq
Reverse (-)
53547737 .. 53551854
4118 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014468

Sequence Viewer

Length: 936 bp
ATGGAATTCATTCCCGAAGAAGGCACACACAACCACGCCGACTCCTCCACTCTAATCCTGCCGGCGCTGTCGATCGGAAATGTGGGCCAGCTCGCCGTGGATCTCTTAGTCGCGTCGACGAAGGCTGAGAGAGTCGGGTTTCTGGATAGTCCGTATGTCCTTTCATGTGTTGGCAACGATGCATATGGGCCTGTTCCTCAAGGCCACCTTGCTCTTCCTCTTGAAGCTTATGAATCGGCTTCTAACGGAATGGCTTTAATCCAGCAAAGGTCACCTGTTGTTAAGGGTAGGATGATTGAGTTTGCTAAGAAGTTGGCTGATTATGTTGCTGCCAGTGGGAAGAAGCATGTGATTGTGCTTTCGAGCTTGGACTTTGGGAGGTGGCAGAGAATTGATATGTCAAGTGGTTCCCAGATGCATTACCTGTCTAGTAGCAATGCCGATGGAACAGATGATTACTGCGAAAAGCTTGGGTGGAAAAGACTTCAGGATTATAACCCCAATCAGAGGTCATGGAAGTATCTTAACACATTAGCTGATGGCAATGCCATGCCAGAGGACAGCTTACCTTTTGAAGGTGAATTGGAAGAAGAAGAATACTACTACCCAAGCTTGCCTTTTGCAGCTCTCTTTTCTTCCTTCAAGGCAAAAGGATTGAAGGTCACCTGTATATTATGCTACTGCTCAGAAGGAGACAACACACCTGATGCTTTCCATTTAGCTGAAGCAGCATGCAAACTTCTGGGTAAATTGAAGGCGTTTTCAAAGGCTGACAAGATTTGGGCAGATGGGAGGTCATTACCTGATCAGAAGCTCAATTTAGTGTCACCTCCAGCAATGTCGTTTTGTAATTCCTTGTTCCTCATTATCCGCTTTGAATACAAAGATGGGATCATGAGCCAAGATATATCTTTAGTGTTAGCTTATTCAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC2

Protein Analysis

312

Amino Acids

34.22

Weight (kDa)

5.12

Isoelectric Point (pI)

51.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAC2 PF09754 18 - 249 5.4e-24 PAC2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
AccI GTMKAC 1 cut(s) 116
AccII CGCG 1 cut(s) 113
AciI CCGC 1 cut(s) 871
AclWI GGATC 2 cut(s) 108, 899
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 2 cut(s) 470, 744
AfiI CCNNNNNNNGG 3 cut(s) 20, 507, 575
AgsI TTSAA 7 cut(s) 224, 575, 643, 658, 754, 765, 878
Alw26I GTCTC 1 cut(s) 687
AlwI GGATC 2 cut(s) 108, 899
AoxI GGCC 3 cut(s) 85, 188, 202
ApeKI GCWGC 3 cut(s) 329, 623, 728
ApoI RAATTY 1 cut(s) 5
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 2 cut(s) 85, 188
AsuHPI GGTGA 4 cut(s) 264, 590, 655, 819
BbvI GCAGC 3 cut(s) 316, 635, 740
BccI CCATC 4 cut(s) 437, 533, 782, 881
BceAI ACGGC 1 cut(s) 80
BclI TGATCA 1 cut(s) 805
BcoDI GTCTC 1 cut(s) 687
BfaI CTAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 68
BisI GCNGC 3 cut(s) 330, 624, 729
BlsI GCNGC 3 cut(s) 331, 625, 730
BmgT120I GGNCC 2 cut(s) 85, 188
BmiI GGNNCC 1 cut(s) 409
BmsI GCATC 3 cut(s) 169, 405, 697
BpmI CTGGAG 1 cut(s) 816
BpuEI CTTGAG 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 96
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 507, 575
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 333
Bse3DI GCAATG 3 cut(s) 442, 550, 843
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 1 cut(s) 297
BseLI CCNNNNNNNGG 3 cut(s) 20, 507, 575
BseMI GCAATG 3 cut(s) 442, 550, 843
BseMII CTCAG 2 cut(s) 117, 699
BseNI ACTGG 1 cut(s) 333
BseRI GAGGAG 1 cut(s) 34
BseXI GCAGC 3 cut(s) 316, 635, 740
Bsh1236I CGCG 1 cut(s) 113
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 3 cut(s) 87, 190, 204
BsiEI CGRYCG 1 cut(s) 75
BsiSI CCGG 1 cut(s) 62
BslI CCNNNNNNNGG 3 cut(s) 20, 507, 575
BsmAI GTCTC 1 cut(s) 687
BsnI GGCC 3 cut(s) 87, 190, 204
Bsp143I GATC 4 cut(s) 72, 100, 805, 891
BspACI CCGC 1 cut(s) 871
BspANI GGCC 3 cut(s) 87, 190, 204
BspCNI CTCAG 2 cut(s) 118, 698
BspFNI CGCG 1 cut(s) 113
BspHI TCATGA 1 cut(s) 894
BspLI GGNNCC 1 cut(s) 409
BspPI GGATC 2 cut(s) 108, 899
BspQI GCTCTTC 1 cut(s) 219
BsrDI GCAATG 3 cut(s) 442, 550, 843
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 4 cut(s) 72, 100, 805, 891
Bst6I CTCTTC 1 cut(s) 219
BstC8I GCNNGC 5 cut(s) 63, 89, 93, 614, 733
BstDEI CTNAG 4 cut(s) 106, 126, 306, 685
BstDSI CCRYGG 1 cut(s) 96
BstEII GGTNACC 2 cut(s) 270, 661
BstENI CCTNNNNNAGG 1 cut(s) 573
BstF5I GGATG 1 cut(s) 297
BstFNI CGCG 1 cut(s) 113
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 4 cut(s) 75, 103, 808, 894
BstMAI GTCTC 1 cut(s) 687
BstMBI GATC 4 cut(s) 72, 100, 805, 891
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 1 cut(s) 728
BstNSI RCATGY 2 cut(s) 350, 735
BstPI GGTNACC 2 cut(s) 270, 661
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 3 cut(s) 316, 635, 740
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 3 cut(s) 87, 190, 204
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 297
BtsIMutI CAGTG 2 cut(s) 340, 936
Cac8I GCNNGC 5 cut(s) 63, 89, 93, 614, 733
CciI TCATGA 1 cut(s) 894
CfoI GCGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 2 cut(s) 85, 188
CseI GACGC 1 cut(s) 102
CviAII CATG 6 cut(s) 165, 347, 513, 550, 732, 895
DdeI CTNAG 4 cut(s) 106, 126, 306, 685
DpnI GATC 4 cut(s) 74, 102, 807, 893
DpnII GATC 4 cut(s) 72, 100, 805, 891
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco57I CTGAAG 2 cut(s) 470, 744
Eco91I GGTNACC 2 cut(s) 270, 661
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoO65I GGTNACC 2 cut(s) 270, 661
EcoRI GAATTC 1 cut(s) 5
EcoT22I ATGCAT 2 cut(s) 184, 420
FaeI CATG 6 cut(s) 168, 350, 516, 553, 735, 898
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 601, 633
FatI CATG 6 cut(s) 164, 346, 512, 549, 731, 894
FauNDI CATATG 1 cut(s) 184
FbaI TGATCA 1 cut(s) 805
FblI GTMKAC 1 cut(s) 116
Fnu4HI GCNGC 3 cut(s) 330, 624, 729
FokI GGATG 1 cut(s) 304
Fsp4HI GCNGC 3 cut(s) 330, 624, 729
FspBI CTAG 1 cut(s) 429
GlaI GCGC 1 cut(s) 66
GluI GCNGC 3 cut(s) 330, 624, 729
GsuI CTGGAG 1 cut(s) 816
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 3 cut(s) 87, 190, 204
HapII CCGG 1 cut(s) 62
HgaI GACGC 1 cut(s) 102
HhaI GCGC 1 cut(s) 67
Hin1II CATG 6 cut(s) 168, 350, 516, 553, 735, 898
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HindIII AAGCTT 3 cut(s) 225, 467, 610
HinfI GANTC 3 cut(s) 41, 132, 233
HpaII CCGG 1 cut(s) 62
HphI GGTGA 4 cut(s) 264, 590, 655, 819
Hpy166II GTNNAC 1 cut(s) 117
Hpy188I TCNGA 4 cut(s) 77, 507, 688, 810
Hpy188III TCNNGA 5 cut(s) 14, 143, 221, 488, 895
Hpy8I GTNNAC 1 cut(s) 117
Hpy99I CGWCG 2 cut(s) 118, 121
HpyAV CCTTC 7 cut(s) 14, 115, 569, 649, 652, 683, 748
HpyCH4V TGCA 4 cut(s) 182, 418, 623, 735
HpyF10VI GCNNNNNNNGC 1 cut(s) 728
HpyF3I CTNAG 4 cut(s) 106, 126, 306, 685
Hsp92II CATG 6 cut(s) 168, 350, 516, 553, 735, 898
HspAI GCGC 1 cut(s) 65
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 4 cut(s) 72, 100, 805, 891
LguI GCTCTTC 1 cut(s) 219
Lsp1109I GCAGC 3 cut(s) 316, 635, 740
LweI GCATC 3 cut(s) 169, 405, 697
MaeI CTAG 1 cut(s) 429
MaeIII GTNAC 3 cut(s) 270, 661, 825
MalI GATC 4 cut(s) 74, 102, 807, 893
MboI GATC 4 cut(s) 72, 100, 805, 891
MboII GAAGA 7 cut(s) 29, 206, 352, 599, 602, 605, 627
MflI RGATCY 1 cut(s) 100
MluCI AATT 6 cut(s) 5, 390, 581, 749, 817, 850
MlyI GAGTC 2 cut(s) 35, 141
MnlI CCTC 9 cut(s) 55, 207, 228, 372, 501, 550, 786, 840, 872
Mph1103I ATGCAT 2 cut(s) 184, 420
MroNI GCCGGC 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 3 cut(s) 257, 282, 525
MspI CCGG 1 cut(s) 62
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 728
NaeI GCCGGC 1 cut(s) 63
NdeI CATATG 1 cut(s) 184
NdeII GATC 4 cut(s) 72, 100, 805, 891
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 6 cut(s) 168, 350, 516, 553, 735, 898
NlaIV GGNNCC 1 cut(s) 409
NmuCI GTSAC 3 cut(s) 270, 661, 825
NsiI ATGCAT 2 cut(s) 184, 420
NspI RCATGY 2 cut(s) 350, 735
PaeI GCATGC 1 cut(s) 735
PagI TCATGA 1 cut(s) 894
PciSI GCTCTTC 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 3 cut(s) 331, 625, 730
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 2 cut(s) 35, 140
PpsI GAGTC 2 cut(s) 35, 140
PsiI TTATAA 1 cut(s) 495
PspEI GGTNACC 2 cut(s) 270, 661
PspN4I GGNNCC 1 cut(s) 409
PspPI GGNCC 2 cut(s) 85, 188
PsuI RGATCY 1 cut(s) 100
PvuI CGATCG 1 cut(s) 75
SalI GTCGAC 1 cut(s) 115
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 3 cut(s) 257, 282, 525
SatI GCNGC 3 cut(s) 330, 624, 729
Sau3AI GATC 4 cut(s) 72, 100, 805, 891
Sau96I GGNCC 2 cut(s) 85, 188
SchI GAGTC 2 cut(s) 35, 141
SfaNI GCATC 3 cut(s) 169, 405, 697
SgrDI CGTCGACG 1 cut(s) 115
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
SphI GCATGC 1 cut(s) 735
Sse9I AATT 6 cut(s) 5, 390, 581, 749, 817, 850
SsiI CCGC 1 cut(s) 871
SspMI CTAG 1 cut(s) 429
TaqI TCGA 3 cut(s) 71, 116, 362
TasI AATT 6 cut(s) 5, 390, 581, 749, 817, 850
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 3 cut(s) 257, 282, 525
Tru9I TTAA 3 cut(s) 257, 282, 525
TscAI CASTG 2 cut(s) 340, 936
TseFI GTSAC 3 cut(s) 270, 661, 825
TseI GCWGC 3 cut(s) 329, 623, 728
Tsp45I GTSAC 3 cut(s) 270, 661, 825
TspDTI ATGAA 2 cut(s) 153, 246
TspGWI ACGGA 2 cut(s) 141, 261
TspRI CASTG 2 cut(s) 340, 936
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 1 cut(s) 5
XceI RCATGY 2 cut(s) 350, 735
XmiI GTMKAC 1 cut(s) 116
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 1 cut(s) 429
Zsp2I ATGCAT 2 cut(s) 184, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.