RLG00000018640

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002770
Physical Location & Seq
Forward (+)
33156467 .. 33159916
3450 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018640

Sequence Viewer

Length: 564 bp
ATGGAATCCATTCCCGAAGAAGGCACACACAACCACGCCGACTTCCCCATTCTAATCTTCCTGCCGGCACTGTCGATCGGAAATGTGGGCCAGCTCGTCGTAGATCTCTTAGTCGCGTCGACAAAGGCGGAGAGAGTCGGGTTTCTGGATAGTCCGTATGTCCTTCCATGCGTTGGCAACGATGCATATGGGCCTGTTCCTCAAGGCCACCTTGCTCTTCTTCTTGAATTTTCAAATGGTAATTCTAAGCAGGGTAGGATGATTGAGTTTGCTAAAAAGTTGGCTGATTATGTTGCTGCCAGTGGGAAGAAGCATGTGATTGTGCTTTCGAGTACAGATAGCATTAATCTTGTCCTCTCTTTGCTTTTATTGAATCAGGCAAAAGGATTGAAGGTCACCTGTTTATTCTGCTACTGCTCAGAAGGAGACAACACCCCTGATGCTTTCCATTTGGCTGAAGCAGCATGCAAACTTATGGGCGTGAGTCCCAGTAATTTCCAGGTCTATCTTTTCAAAGTAAAATTAGAATCATTAGGTATCTTGTCACAGGACAGTTACGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC2

Protein Analysis

188

Amino Acids

20.09

Weight (kDa)

5.45

Isoelectric Point (pI)

30.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAC2 PF09754 20 - 113 4.3e-14 PAC2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 173
AccI GTMKAC 1 cut(s) 119
AccII CGCG 1 cut(s) 116
AciI CCGC 1 cut(s) 128
AcsI RAATTY 1 cut(s) 227
AcuI CTGAAG 1 cut(s) 477
AfaI GTAC 1 cut(s) 334
AfiI CCNNNNNNNGG 2 cut(s) 20, 173
AgsI TTSAA 5 cut(s) 227, 234, 373, 391, 514
AjnI CCWGG 1 cut(s) 498
AluBI AGCT 1 cut(s) 94
AluI AGCT 1 cut(s) 94
Alw26I GTCTC 1 cut(s) 420
AoxI GGCC 3 cut(s) 88, 191, 205
ApeKI GCWGC 2 cut(s) 296, 461
ApoI RAATTY 1 cut(s) 227
AseI ATTAAT 1 cut(s) 345
Asp700I GAANNNNTTC 1 cut(s) 9
AspS9I GGNCC 2 cut(s) 88, 191
AsuHPI GGTGA 1 cut(s) 388
BbvI GCAGC 2 cut(s) 283, 473
BciT130I CCWGG 1 cut(s) 500
BcoDI GTCTC 1 cut(s) 420
BglII AGATCT 1 cut(s) 103
BisI GCNGC 2 cut(s) 297, 462
BlsI GCNGC 2 cut(s) 298, 463
Bme1390I CCNGG 1 cut(s) 500
BmgT120I GGNCC 2 cut(s) 88, 191
BmrFI CCNGG 1 cut(s) 500
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 2 cut(s) 172, 430
BmuI ACTGGG 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 186
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 173
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 2 cut(s) 300, 489
BseBI CCWGG 1 cut(s) 500
BseGI GGATG 1 cut(s) 264
BseLI CCNNNNNNNGG 2 cut(s) 20, 173
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 2 cut(s) 300, 489
BseXI GCAGC 2 cut(s) 283, 473
Bsh1236I CGCG 1 cut(s) 116
Bsh1285I CGRYCG 1 cut(s) 78
BshFI GGCC 3 cut(s) 90, 193, 207
BsiEI CGRYCG 1 cut(s) 78
BsiSI CCGG 1 cut(s) 65
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 2 cut(s) 20, 173
BsmAI GTCTC 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 471
BsnI GGCC 3 cut(s) 90, 193, 207
Bsp143I GATC 2 cut(s) 75, 103
BspACI CCGC 1 cut(s) 128
BspANI GGCC 3 cut(s) 90, 193, 207
BspCNI CTCAG 1 cut(s) 431
BspFNI CGCG 1 cut(s) 116
BspQI GCTCTTC 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 2 cut(s) 300, 489
BssAI RCCGGY 1 cut(s) 64
BssMI GATC 2 cut(s) 75, 103
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 2 cut(s) 72, 554
Bst6I CTCTTC 1 cut(s) 222
BstC8I GCNNGC 3 cut(s) 66, 92, 466
BstDEI CTNAG 3 cut(s) 109, 246, 418
BstEII GGTNACC 1 cut(s) 394
BstF5I GGATG 1 cut(s) 264
BstFNI CGCG 1 cut(s) 116
BstKTI GATC 2 cut(s) 78, 106
BstMAI GTCTC 1 cut(s) 420
BstMBI GATC 2 cut(s) 75, 103
BstMCI CGRYCG 1 cut(s) 78
BstMWI GCNNNNNNNGC 1 cut(s) 461
BstNI CCWGG 1 cut(s) 500
BstNSI RCATGY 2 cut(s) 317, 468
BstPI GGTNACC 1 cut(s) 394
BstSCI CCNGG 1 cut(s) 498
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 2 cut(s) 283, 473
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 3 cut(s) 90, 193, 207
BtsCI GGATG 1 cut(s) 264
BtsIMutI CAGTG 2 cut(s) 68, 307
Cac8I GCNNGC 3 cut(s) 66, 92, 466
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 2 cut(s) 88, 191
CseI GACGC 1 cut(s) 105
Csp6I GTAC 1 cut(s) 333
CviAII CATG 3 cut(s) 168, 314, 465
CviJI RGCY 6 cut(s) 90, 94, 193, 207, 284, 455
CviKI_1 RGCY 6 cut(s) 90, 94, 193, 207, 284, 455
CviQI GTAC 1 cut(s) 333
DdeI CTNAG 3 cut(s) 109, 246, 418
DpnI GATC 2 cut(s) 77, 105
DpnII GATC 2 cut(s) 75, 103
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
EciI GGCGGA 1 cut(s) 143
Eco57I CTGAAG 1 cut(s) 477
Eco91I GGTNACC 1 cut(s) 394
EcoO65I GGTNACC 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 498
EcoT22I ATGCAT 1 cut(s) 187
FaeI CATG 3 cut(s) 171, 317, 468
FaiI YATR 9 cut(s) 159, 169, 187, 189, 291, 315, 466, 476, 562
FalI AAGNNNNNCTT 2 cut(s) 195, 227
FaqI GGGAC 1 cut(s) 471
FatI CATG 3 cut(s) 167, 313, 464
FauNDI CATATG 1 cut(s) 187
FblI GTMKAC 1 cut(s) 119
Fnu4HI GCNGC 2 cut(s) 297, 462
FokI GGATG 1 cut(s) 271
Fsp4HI GCNGC 2 cut(s) 297, 462
GluI GCNGC 2 cut(s) 297, 462
HaeIII GGCC 3 cut(s) 90, 193, 207
HapII CCGG 1 cut(s) 65
HgaI GACGC 1 cut(s) 105
Hin1II CATG 3 cut(s) 171, 317, 468
HincII GTYRAC 1 cut(s) 120
HindII GTYRAC 1 cut(s) 120
HinfI GANTC 5 cut(s) 5, 135, 373, 484, 527
HpaII CCGG 1 cut(s) 65
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 1 cut(s) 120
Hpy188I TCNGA 2 cut(s) 80, 421
Hpy188III TCNNGA 3 cut(s) 14, 146, 224
Hpy8I GTNNAC 1 cut(s) 120
Hpy99I CGWCG 2 cut(s) 101, 121
HpyAV CCTTC 4 cut(s) 14, 173, 385, 416
HpyCH4III ACNGT 2 cut(s) 72, 554
HpyCH4V TGCA 2 cut(s) 185, 468
HpyF10VI GCNNNNNNNGC 1 cut(s) 461
HpyF3I CTNAG 3 cut(s) 109, 246, 418
Hsp92II CATG 3 cut(s) 171, 317, 468
KroI GCCGGC 1 cut(s) 64
KroNI GCCGGC 1 cut(s) 66
Kzo9I GATC 2 cut(s) 75, 103
LguI GCTCTTC 1 cut(s) 222
Lsp1109I GCAGC 2 cut(s) 283, 473
LweI GCATC 2 cut(s) 172, 430
MaeIII GTNAC 3 cut(s) 394, 543, 554
MalI GATC 2 cut(s) 77, 105
MboI GATC 2 cut(s) 75, 103
MboII GAAGA 5 cut(s) 29, 49, 209, 212, 319
MflI RGATCY 1 cut(s) 103
MluCI AATT 4 cut(s) 227, 241, 493, 521
MlyI GAGTC 2 cut(s) 144, 493
MnlI CCTC 2 cut(s) 210, 365
Mph1103I ATGCAT 1 cut(s) 187
MroNI GCCGGC 1 cut(s) 64
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 1 cut(s) 345
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 500
MvaI CCWGG 1 cut(s) 500
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 1 cut(s) 461
NaeI GCCGGC 1 cut(s) 66
NdeI CATATG 1 cut(s) 187
NdeII GATC 2 cut(s) 75, 103
NgoMIV GCCGGC 1 cut(s) 64
NlaIII CATG 3 cut(s) 171, 317, 468
NmuCI GTSAC 2 cut(s) 394, 543
NsiI ATGCAT 1 cut(s) 187
NspI RCATGY 2 cut(s) 317, 468
PaeI GCATGC 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 222
PdiI GCCGGC 1 cut(s) 66
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 3 cut(s) 5, 373, 527
PflMI CCANNNNNTGG 1 cut(s) 173
PkrI GCNGC 2 cut(s) 298, 463
Ple19I CGATCG 1 cut(s) 78
PleI GAGTC 2 cut(s) 143, 492
PpsI GAGTC 2 cut(s) 143, 492
PshBI ATTAAT 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 498
PspEI GGTNACC 1 cut(s) 394
PspGI CCWGG 1 cut(s) 498
PspPI GGNCC 2 cut(s) 88, 191
PsuI RGATCY 1 cut(s) 103
PvuI CGATCG 1 cut(s) 78
RsaI GTAC 1 cut(s) 334
RsaNI GTAC 1 cut(s) 333
SalI GTCGAC 1 cut(s) 118
SapI GCTCTTC 1 cut(s) 222
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 2 cut(s) 297, 462
Sau3AI GATC 2 cut(s) 75, 103
Sau96I GGNCC 2 cut(s) 88, 191
SchI GAGTC 2 cut(s) 144, 493
ScrFI CCNGG 1 cut(s) 500
SetI ASST 6 cut(s) 96, 213, 396, 401, 504, 538
SfaNI GCATC 2 cut(s) 172, 430
SmlI CTYRAG 1 cut(s) 201
SmoI CTYRAG 1 cut(s) 201
SphI GCATGC 1 cut(s) 468
Sse9I AATT 4 cut(s) 227, 241, 493, 521
SsiI CCGC 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 498
TaaI ACNGT 2 cut(s) 72, 554
TaqI TCGA 3 cut(s) 74, 119, 329
TasI AATT 4 cut(s) 227, 241, 493, 521
TatI WGTACW 1 cut(s) 332
TfiI GAWTC 3 cut(s) 5, 373, 527
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 2 cut(s) 75, 307
TseFI GTSAC 2 cut(s) 394, 543
TseI GCWGC 2 cut(s) 296, 461
Tsp45I GTSAC 2 cut(s) 394, 543
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 2 cut(s) 75, 307
Van91I CCANNNNNTGG 1 cut(s) 173
VspI ATTAAT 1 cut(s) 345
XapI RAATTY 1 cut(s) 227
XceI RCATGY 2 cut(s) 317, 468
XmiI GTMKAC 1 cut(s) 119
XmnI GAANNNNTTC 1 cut(s) 9
Zsp2I ATGCAT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.