pycom10g14210

Cyclin-dependent kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002441
Physical Location & Seq
Reverse (-)
17581229 .. 17583307
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
ATGGGGAAATACATGAAGAAATCGAAAATTTCCGGCGACGTAGCCGTGATGGAGGTCTCCATTTCGCCGCAGTCCTCTCTCGGCGTTCGGACCAGAGCCAAAACCCTAGCCCTCCAAAAGCTCCAGCAGCAGCAGCAGCAGCAGAAGCAAGAGAATCAAGATCAATCCCCCGCCGGAGAATCGGACTCAGATAGCTCCTCCCTGTACTACCTCCAGCTGAGGAGCCGCCGTCTAGAGAAGCCCCCGTTGCGAAACGACGCCGTCAAGAAGCAGTGCCAGAGCGGCGGAATTAATGAGCCCTCGGAGAAGCCCAGAGGAGGCTGCTGCGGGGAAAGCCGACGGCGGCCGGTGGAATCGAGGCCCAAATCCAGGCTGAGCGTGGGCTCGTCGGTGGAGTTGGACTGGGCTGGACCCGTTGAGAGGAAAGAGAAGGAAGGGGAGGAGGGGTGTTTTGGTAATTTCGGAAGTGGAGCAGAGTATGGTAACGATTTGGGGGTCGAGGCTTCGTTTGGGGAAAACAACTTGGATTTTGAAGCCAGAGACAGGAGCACTAGAGAAACCACTCCGTGCAGCTTCATAAGGGAGTCAGACACAATAGGAACCCCCGGTTCCACCACAAGGCGGACGAGTTCTATTTCAACTCACCGGAGAGTTAGAAACGATGTGCAAAGAAATATCCCAACCACGCTTGAGATGGAGGAGTTCTTTGCCCGACATGAGGAGGAGCAACAGAAAATCTTCATTGAGAAGTACAACTTTGACATCATCAACGATTTACCCCTTCCCGGAAGGTATGAATGGGAGCAAGTGATTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDI

Protein Analysis

273

Amino Acids

30.7

Weight (kDa)

6.28

Isoelectric Point (pI)

67.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDI PF02234 224 - 270 4e-19 Cyclin-dependent kinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 282
AciI CCGC 8 cut(s) 68, 171, 226, 282, 285, 327, 343, 622
AcoI YGGCCR 1 cut(s) 344
AcsI RAATTY 1 cut(s) 27
AcyI GRCGYC 1 cut(s) 258
AdeI CACNNNGTG 1 cut(s) 567
AfaI GTAC 2 cut(s) 206, 752
AfiI CCNNNNNNNGG 8 cut(s) 317, 369, 420, 543, 618, 621, 718, 785
AgsI TTSAA 2 cut(s) 533, 639
AjnI CCWGG 1 cut(s) 368
AluBI AGCT 4 cut(s) 121, 195, 217, 573
AluI AGCT 4 cut(s) 121, 195, 217, 573
Alw21I GWGCWC 1 cut(s) 551
Alw26I GTCTC 2 cut(s) 61, 534
AoxI GGCC 2 cut(s) 344, 359
ApeKI GCWGC 8 cut(s) 127, 130, 133, 136, 139, 321, 324, 570
ApoI RAATTY 1 cut(s) 27
AseI ATTAAT 1 cut(s) 291
Asp700I GAANNNNTTC 1 cut(s) 737
AspS9I GGNCC 3 cut(s) 90, 360, 410
AsuC2I CCSGG 2 cut(s) 606, 786
AsuHPI GGTGA 1 cut(s) 635
AvaII GGWCC 2 cut(s) 90, 410
BanII GRGCYC 2 cut(s) 300, 386
Bbv12I GWGCWC 1 cut(s) 551
BbvCI CCTCAGC 1 cut(s) 218
BbvI GCAGC 8 cut(s) 139, 142, 145, 148, 151, 308, 311, 582
BccI CCATC 2 cut(s) 43, 688
BceAI ACGGC 4 cut(s) 29, 213, 245, 356
BciT130I CCWGG 1 cut(s) 370
BcnI CCSGG 2 cut(s) 606, 786
BcoDI GTCTC 2 cut(s) 61, 534
BfaI CTAG 3 cut(s) 107, 233, 552
BglI GCCNNNNNGGC 1 cut(s) 282
BlpI GCTNAGC 1 cut(s) 374
Bme1390I CCNGG 3 cut(s) 370, 606, 786
Bme18I GGWCC 2 cut(s) 90, 410
BmgT120I GGNCC 3 cut(s) 90, 360, 410
BmiI GGNNCC 4 cut(s) 224, 412, 601, 610
BmrFI CCNGG 3 cut(s) 370, 606, 786
BmrI ACTGGG 1 cut(s) 412
BmuI ACTGGG 1 cut(s) 412
BpmI CTGGAG 2 cut(s) 107, 197
Bpu10I CCTNAGC 1 cut(s) 218
Bpu1102I GCTNAGC 1 cut(s) 374
BpuEI CTTGAG 1 cut(s) 710
BpuMI CCSGG 2 cut(s) 606, 786
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 2 cut(s) 300, 604
BsaWI WCCGGW 1 cut(s) 645
BsaXI ACNNNNNCTCC 4 cut(s) 214, 244, 431, 461
Bsc4I CCNNNNNNNGG 8 cut(s) 317, 369, 420, 543, 618, 621, 718, 785
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 1 cut(s) 407
BseBI CCWGG 1 cut(s) 370
BseDI CCNNGG 2 cut(s) 300, 604
BseLI CCNNNNNNNGG 8 cut(s) 317, 369, 420, 543, 618, 621, 718, 785
BseMII CTCAG 3 cut(s) 201, 209, 365
BseNI ACTGG 1 cut(s) 407
BseRI GAGGAG 7 cut(s) 187, 235, 330, 455, 713, 734, 737
BseX3I CGGCCG 1 cut(s) 344
BseXI GCAGC 8 cut(s) 139, 142, 145, 148, 151, 308, 311, 582
BsgI GTGCAG 1 cut(s) 589
Bsh1285I CGRYCG 1 cut(s) 347
BshFI GGCC 2 cut(s) 346, 361
BsiEI CGRYCG 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 551
BsiSI CCGG 6 cut(s) 33, 174, 347, 606, 646, 786
BslI CCNNNNNNNGG 8 cut(s) 317, 369, 420, 543, 618, 621, 718, 785
BsmAI GTCTC 2 cut(s) 61, 534
BsnI GGCC 2 cut(s) 346, 361
Bso31I GGTCTC 1 cut(s) 61
Bsp1286I GDGCHC 3 cut(s) 300, 386, 551
Bsp143I GATC 1 cut(s) 160
Bsp1720I GCTNAGC 1 cut(s) 374
BspACI CCGC 8 cut(s) 68, 171, 226, 282, 285, 327, 343, 622
BspANI GGCC 2 cut(s) 346, 361
BspCNI CTCAG 3 cut(s) 200, 210, 366
BspLI GGNNCC 4 cut(s) 224, 412, 601, 610
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 1 cut(s) 282
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 300, 604
BssMI GATC 1 cut(s) 160
BssNI GRCGYC 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 370
BstACI GRCGYC 1 cut(s) 258
BstDEI CTNAG 3 cut(s) 187, 218, 374
BstKTI GATC 1 cut(s) 163
BstMAI GTCTC 2 cut(s) 61, 534
BstMBI GATC 1 cut(s) 160
BstMCI CGRYCG 1 cut(s) 347
BstMWI GCNNNNNNNGC 8 cut(s) 127, 133, 136, 139, 145, 247, 282, 333
BstNI CCWGG 1 cut(s) 370
BstSCI CCNGG 3 cut(s) 368, 604, 784
BstV1I GCAGC 8 cut(s) 139, 142, 145, 148, 151, 308, 311, 582
BstZI CGGCCG 1 cut(s) 344
BsuRI GGCC 2 cut(s) 346, 361
BtsI GCAGTG 1 cut(s) 278
BtsIMutI CAGTG 1 cut(s) 278
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 3 cut(s) 90, 360, 410
CseI GACGC 1 cut(s) 266
Csp6I GTAC 2 cut(s) 205, 751
CviAII CATG 2 cut(s) 13, 716
CviQI GTAC 2 cut(s) 205, 751
DdeI CTNAG 3 cut(s) 187, 218, 374
DpnI GATC 1 cut(s) 162
DpnII GATC 1 cut(s) 160
DraIII CACNNNGTG 1 cut(s) 567
EaeI YGGCCR 1 cut(s) 344
EagI CGGCCG 1 cut(s) 344
EciI GGCGGA 2 cut(s) 300, 637
EclXI CGGCCG 1 cut(s) 344
Eco24I GRGCYC 2 cut(s) 300, 386
Eco31I GGTCTC 1 cut(s) 61
Eco47I GGWCC 2 cut(s) 90, 410
Eco52I CGGCCG 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 368
EcoT38I GRGCYC 2 cut(s) 300, 386
FaeI CATG 2 cut(s) 16, 719
FaiI YATR 5 cut(s) 14, 480, 578, 717, 795
FalI AAGNNNNNCTT 2 cut(s) 740, 772
FatI CATG 2 cut(s) 12, 715
FauI CCCGC 2 cut(s) 178, 320
FriOI GRGCYC 2 cut(s) 300, 386
FspBI CTAG 3 cut(s) 107, 233, 552
GsuI CTGGAG 2 cut(s) 107, 197
HaeIII GGCC 2 cut(s) 346, 361
HapII CCGG 6 cut(s) 33, 174, 347, 606, 646, 786
HgaI GACGC 1 cut(s) 266
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 2 cut(s) 16, 719
HinfI GANTC 6 cut(s) 154, 179, 185, 353, 584, 811
HpaII CCGG 6 cut(s) 33, 174, 347, 606, 646, 786
HphI GGTGA 1 cut(s) 635
Hpy188I TCNGA 6 cut(s) 90, 184, 190, 304, 464, 589
Hpy188III TCNNGA 3 cut(s) 158, 233, 265
Hpy99I CGWCG 4 cut(s) 41, 260, 342, 391
HpyAV CCTTC 4 cut(s) 424, 428, 783, 791
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 2 cut(s) 570, 667
HpyF10VI GCNNNNNNNGC 8 cut(s) 127, 133, 136, 139, 145, 247, 282, 333
HpyF3I CTNAG 3 cut(s) 187, 218, 374
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 2 cut(s) 16, 719
Kzo9I GATC 1 cut(s) 160
LmnI GCTCC 7 cut(s) 126, 200, 222, 470, 546, 724, 802
Lsp1109I GCAGC 8 cut(s) 139, 142, 145, 148, 151, 308, 311, 582
MaeI CTAG 3 cut(s) 107, 233, 552
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 482
MalI GATC 1 cut(s) 162
MbiI CCGCTC 1 cut(s) 282
MboI GATC 1 cut(s) 160
MboII GAAGA 2 cut(s) 28, 730
MhlI GDGCHC 3 cut(s) 300, 386, 551
MluCI AATT 3 cut(s) 27, 288, 457
MlyI GAGTC 2 cut(s) 179, 593
MmeI TCCRAC 1 cut(s) 378
MroXI GAANNNNTTC 1 cut(s) 737
MseI TTAA 1 cut(s) 291
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 6 cut(s) 33, 174, 347, 606, 646, 786
MspR9I CCNGG 3 cut(s) 370, 606, 786
MvaI CCWGG 1 cut(s) 370
MwoI GCNNNNNNNGC 8 cut(s) 127, 133, 136, 139, 145, 247, 282, 333
NciI CCSGG 2 cut(s) 606, 786
NdeII GATC 1 cut(s) 160
NlaIII CATG 2 cut(s) 16, 719
NlaIV GGNNCC 4 cut(s) 224, 412, 601, 610
NmeAIII GCCGAG 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 737
PfeI GAWTC 4 cut(s) 154, 179, 353, 811
PflFI GACNNNGTC 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 784
PleI GAGTC 2 cut(s) 179, 592
PpsI GAGTC 2 cut(s) 179, 592
PshBI ATTAAT 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 368
PspGI CCWGG 1 cut(s) 368
PspN4I GGNNCC 4 cut(s) 224, 412, 601, 610
PspPI GGNCC 3 cut(s) 90, 360, 410
PsyI GACNNNGTC 1 cut(s) 260
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 2 cut(s) 206, 752
RsaNI GTAC 2 cut(s) 205, 751
SaqAI TTAA 1 cut(s) 291
Sau3AI GATC 1 cut(s) 160
Sau96I GGNCC 3 cut(s) 90, 360, 410
SchI GAGTC 2 cut(s) 179, 593
ScrFI CCNGG 3 cut(s) 370, 606, 786
SduI GDGCHC 3 cut(s) 300, 386, 551
SetI ASST 8 cut(s) 42, 57, 123, 197, 213, 219, 575, 794
SinI GGWCC 2 cut(s) 90, 410
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
Sse9I AATT 3 cut(s) 27, 288, 457
SsiI CCGC 8 cut(s) 68, 171, 226, 282, 285, 327, 343, 622
SspMI CTAG 3 cut(s) 107, 233, 552
StyD4I CCNGG 3 cut(s) 368, 604, 784
TaiI ACGT 1 cut(s) 42
TaqI TCGA 3 cut(s) 23, 356, 498
TasI AATT 3 cut(s) 27, 288, 457
TatI WGTACW 2 cut(s) 204, 750
TauI GCSGC 4 cut(s) 70, 228, 285, 346
TfiI GAWTC 4 cut(s) 154, 179, 353, 811
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 1 cut(s) 278
TseI GCWGC 8 cut(s) 127, 130, 133, 136, 139, 321, 324, 570
TspDTI ATGAA 4 cut(s) 29, 565, 730, 810
TspGWI ACGGA 1 cut(s) 555
TspRI CASTG 1 cut(s) 278
Tth111I GACNNNGTC 1 cut(s) 260
VpaK11BI GGWCC 2 cut(s) 90, 410
VspI ATTAAT 1 cut(s) 291
XapI RAATTY 1 cut(s) 27
XbaI TCTAGA 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 2 cut(s) 376, 691
XmnI GAANNNNTTC 1 cut(s) 737
XspI CTAG 3 cut(s) 107, 233, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.