pycom10g19390

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000477
Physical Location & Seq
Reverse (-)
22496620 .. 22500830
4211 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2316 bp
ATGTCCTACGAATGGTATGGTGTTTTTTTAGGACTAGCTTTGGTCCTGTTTCTGATTGCTCCTTGTTTACTTCAGACGTATTGTTCAGTGGGAACGGTTCTACAGCTTGCATTTCAAAGTCTTGGTGTGGTCTATGGAGATTTGGGCACATCCCCTTTGTATGTTTTCAAAAATATATTTCCTGCAGGAATCGATGCCCCGGAAGATTTACTCGGAGCTTTATCGGTCATCATATATTCCCTCACTCTTATCGTACTCGTGAAGTATGTTCTTATTGTCTGTAGAGCGAATGACAACGGTCAAGGTGGAACATTTGCTCTTTATTCATTACTCTGTCGACATGCAAAAATAAAGACCGTTCCTAACCAAGACCAAAGTGATGAGGAGCTAACAACATTTAGCCGTTCTACATTTGGTGAGAAATCATTTTCTGAATGCACCAAGCGATGGTTGGAAGGACACGCAGTCATGCGGAACATACTTCTTATTCTTGTCCTGGTTGGCTCTTGCGCTGTGATAGGGGATGGGATTCTTACTCCAGCCATATCAGTTCTGTCAGCGGTCGGTGGGATCAATGTGGGACGGTCCCAGATTAGTAATGACGTAGTTGTACTTGTTTCTGTTGTTATACTGGTACTTTTGTTTATCGTTCAACAATTTGGTCCGGAGAAAGTTGGTTGGCTGTTTGCACCAGTTGTGCTCCTTTGGTTTCTCGTAATTGGAGGAATCGGCATCTTCAACATCTGGAAATATGACACTAGCGTCTTGAAAGCTTTTTCACCCATGTATGTGTATGTATACTTTAAGAAGGGAGGTATAACTGGGTGGGCATCGCTTGGTGGTATCGTGCTTTGTATAAACGGAACGGAAACACTTTTTGCTGATGTATCGCATTTCCCAGTCCTGTCCATACAGATTGCTTTCACTGTTGTTGTATTTCCTTGCCTTCTTTTAGCCTACTCTGGTCAAGCTGCCTACCTCATGAAAAACCGAGATCACGTAATAGATGCCTTTCATTACTCTATTCCAGAGCACATATATTGGCCTGTGTTTGCTGTTGCAACTGCTGCTGCTATTGTTGCAAGTCAGGCCATCATAACCGCCACTTTTTCATTAGTCAAGCAGGCCCTTGCGCTTGGCTGTTTCCCAAGAGTCAAAGTTGTATATACATCGAGGAACCGTCATCAAATATATATTCCGGAGATTAATTGGATTCTTATGATTCTCTGCATCTTAATAACAGTTCGATTTAGAAATCAAGCTGAAATTGGGAACGCTGCCGGGACGGCGGTTGTTATAGTCATGTTGGTAACCACATTTCTGATGATGTTAGTGATGATATTAGTTTGGCGCTGCCATTGGACTCTCGTACTTATCTTCAGTGTCTTAACATTTGTGGTGGAGGGCACTTACCTTTCGGCTGTACTCCCCAAGGTCAATCAAGGAGGCTGGGTTCCTCTTGTGCTTACACTGGCCTTCTTTATCGTCATGTATGGTTGGCATTACGGTACAGAAATACGCTATGACATTGAGATGCACAGCAAGGTGTCGATGGCATGGCTTCTTGGACTTGGTCCCAGTTTAGGATTGGTTCGGGTACCTGGGATAGGCCTTGTGTACTCTGAGATAGCGACTGGAGTCCCCCGCATCTTCTCTCACTTTATCACCAACCTGCCCGCCATCCATTCTGTTGTCGTGTTTGTCTGCGTGAAGTACCTTCCTGTCTGCACAGTCCCAGAAGAAGAACGGTTCCTTGTTAAGCGGATTGGACCCGAAAACTTCCACATGTTCCGTTGTGTTGTAAGATATGGCTACAAGGACGACCACAATAGGGACGACGAATTTGAGAAAAAGCTCTTTGACAATCTCTTCACATTCGTCCGGCTTGAGTCCCTGATGGAAGGATCTCCAGGCTCTGATGTATCTAGTATACTCAACCAGGACGCCATGTGGAACAACCACAGCAGCAGCATACCTTACTCGAATGCAGACCTGTCGATTACATCAGTAGACTCCATCGAACCTGATGATTCTCCGGTGCATATCGGCCAGAGTTTGGCCTACTCCTCTGTCCCGCCGAGCAAGCAGCTGGTGGAGCTCGACGGGATAGAGTTCTTGAACCGGTGTAGAGATGCAGGAGTGGTGCACATACTTGGGAACACTGTGGTACACGCTAGGGGGGGATCAAATTTTTTTAGGAAGGTGGCGATTGATTACCTGTATGCATTTCTTAGGAAGATTTGCAGAGGGAACAGTGTGTTGTTCAATGTTCCTCATGAGAGTCTCTTGAGCGTTGGACAGATTTTCTATGTGTGA

Protein Analysis

772

Amino Acids

85.4

Weight (kDa)

6.67

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 36 - 520 7.7e-156 K+ potassium transporter integral membrane domain
K_trans_C PF22776 534 - 768 3.3e-23 K+ potassium transporter C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 761
Acc36I ACCTGC 1 cut(s) 1680
Acc65I GGTACC 1 cut(s) 1597
AccB1I GGYRCC 1 cut(s) 1597
AccB7I CCANNNNNTGG 2 cut(s) 447, 2056
AccI GTMKAC 4 cut(s) 337, 798, 1930, 2010
AccIII TCCGGA 2 cut(s) 664, 1198
AciI CCGC 8 cut(s) 472, 560, 1101, 1289, 1645, 1677, 1762, 2075
AclWI GGATC 3 cut(s) 578, 1912, 2191
AcoI YGGCCR 1 cut(s) 2047
AcsI RAATTY 2 cut(s) 1841, 2188
AcuI CTGAAG 2 cut(s) 56, 1363
AcyI GRCGYC 1 cut(s) 1944
AfiI CCNNNNNNNGG 6 cut(s) 12, 447, 1583, 1607, 1831, 2056
AflIII ACRYGT 1 cut(s) 1785
AgeI ACCGGT 1 cut(s) 2121
AgsI TTSAA 7 cut(s) 116, 169, 653, 739, 769, 2119, 2266
AhdI GACNNNNNGTC 1 cut(s) 464
AjnI CCWGG 4 cut(s) 495, 1600, 1909, 1938
AloI GAACNNNNNNTCC 2 cut(s) 1437, 1469
Alw21I GWGCWC 4 cut(s) 702, 1035, 2100, 2148
Alw26I GTCTC 1 cut(s) 2288
Alw44I GTGCAC 1 cut(s) 2144
AlwI GGATC 3 cut(s) 578, 1912, 2191
AlwNI CAGNNNCTG 1 cut(s) 1916
Aor13HI TCCGGA 2 cut(s) 664, 1198
AoxI GGCC 7 cut(s) 1043, 1089, 1125, 1473, 1609, 2047, 2058
ApaLI GTGCAC 1 cut(s) 2144
ApeKI GCWGC 8 cut(s) 971, 1067, 1070, 1277, 1353, 1965, 1968, 2086
ApoI RAATTY 2 cut(s) 1841, 2188
ArsI GACNNNNNNTTYG 2 cut(s) 1826, 1858
AseI ATTAAT 1 cut(s) 1206
AsiGI ACCGGT 1 cut(s) 2121
Asp718I GGTACC 1 cut(s) 1597
AspLEI GCGC 3 cut(s) 512, 1135, 1353
AspS9I GGNCC 6 cut(s) 43, 585, 662, 1126, 1574, 1769
AsuC2I CCSGG 2 cut(s) 200, 1282
AsuHPI GGTGA 3 cut(s) 428, 771, 1657
AvaII GGWCC 5 cut(s) 43, 585, 662, 1574, 1769
BaeGI GKGCMC 3 cut(s) 149, 1409, 2148
BaeI ACNNNNGTAYC 2 cut(s) 626, 659
BanI GGYRCC 1 cut(s) 1597
BanII GRGCYC 1 cut(s) 2100
BauI CACGAG 1 cut(s) 257
Bbv12I GWGCWC 4 cut(s) 702, 1035, 2100, 2148
BbvI GCAGC 8 cut(s) 958, 1054, 1057, 1264, 1340, 1977, 1980, 2098
BccI CCATC 7 cut(s) 441, 518, 1100, 1546, 1688, 1891, 2024
BceAI ACGGC 2 cut(s) 387, 1302
BcgI CGANNNNNNTGC 2 cut(s) 1977, 2011
BciT130I CCWGG 4 cut(s) 497, 1602, 1911, 1940
BcnI CCSGG 2 cut(s) 200, 1282
BcoDI GTCTC 1 cut(s) 2288
BfaI CTAG 4 cut(s) 35, 759, 1926, 2175
BfmI CTRYAG 3 cut(s) 101, 183, 280
BfoI RGCGCY 1 cut(s) 1354
BfuAI ACCTGC 1 cut(s) 1680
BglI GCCNNNNNGGC 1 cut(s) 1286
BisI GCNGC 8 cut(s) 972, 1068, 1071, 1278, 1354, 1966, 1969, 2087
BlsI GCNGC 8 cut(s) 973, 1069, 1072, 1279, 1355, 1967, 1970, 2088
Bme1390I CCNGG 6 cut(s) 200, 497, 1282, 1602, 1911, 1940
Bme18I GGWCC 5 cut(s) 43, 585, 662, 1574, 1769
BmeRI GACNNNNNGTC 1 cut(s) 464
BmgT120I GGNCC 6 cut(s) 43, 585, 662, 1126, 1574, 1769
BmiI GGNNCC 7 cut(s) 587, 1178, 1455, 1576, 1599, 1751, 1771
BmrFI CCNGG 6 cut(s) 200, 497, 1282, 1602, 1911, 1940
BmrI ACTGGG 3 cut(s) 831, 893, 1572
BmsI GCATC 8 cut(s) 184, 741, 839, 997, 1239, 1524, 1656, 2122
BmuI ACTGGG 3 cut(s) 831, 893, 1572
BoxI GACNNNNGTC 2 cut(s) 297, 1637
BpmI CTGGAG 3 cut(s) 522, 1656, 1893
BpuEI CTTGAG 2 cut(s) 1907, 2308
BpuMI CCSGG 2 cut(s) 200, 1282
Bsa29I ATCGAT 1 cut(s) 192
BsaAI YACGTR 1 cut(s) 1000
BsaHI GRCGYC 1 cut(s) 1944
BsaJI CCNNGG 3 cut(s) 198, 1431, 1601
BsaWI WCCGGW 4 cut(s) 664, 1198, 2035, 2121
BsaXI ACNNNNNCTCC 2 cut(s) 1437, 1467
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 447, 1583, 1607, 1831, 2056
Bse118I RCCGGY 1 cut(s) 2121
Bse1I ACTGG 7 cut(s) 636, 692, 826, 899, 1476, 1578, 1639
BseAI TCCGGA 2 cut(s) 664, 1198
BseBI CCWGG 4 cut(s) 497, 1602, 1911, 1940
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 3 cut(s) 198, 1431, 1601
BseGI GGATG 3 cut(s) 149, 529, 1680
BseLI CCNNNNNNNGG 6 cut(s) 12, 447, 1583, 1607, 1831, 2056
BseMII CTCAG 1 cut(s) 1614
BseNI ACTGG 7 cut(s) 636, 692, 826, 899, 1476, 1578, 1639
BseRI GAGGAG 2 cut(s) 398, 2056
BseSI GKGCMC 3 cut(s) 149, 1409, 2148
BseXI GCAGC 8 cut(s) 958, 1054, 1057, 1264, 1340, 1977, 1980, 2098
BseYI CCCAGC 1 cut(s) 1449
BsgI GTGCAG 1 cut(s) 1711
Bsh1285I CGRYCG 1 cut(s) 564
BshFI GGCC 7 cut(s) 1045, 1091, 1127, 1475, 1611, 2049, 2060
BshNI GGYRCC 1 cut(s) 1597
BshTI ACCGGT 1 cut(s) 2121
BshVI ATCGAT 1 cut(s) 192
BsiEI CGRYCG 1 cut(s) 564
BsiHKAI GWGCWC 4 cut(s) 702, 1035, 2100, 2148
BsiSI CCGG 7 cut(s) 200, 665, 1199, 1281, 1882, 2036, 2122
BslFI GGGAC 9 cut(s) 571, 594, 1297, 1560, 1625, 1718, 1847, 1876, 2057
BslI CCNNNNNNNGG 6 cut(s) 12, 447, 1583, 1607, 1831, 2056
BsmAI GTCTC 1 cut(s) 2288
BsmFI GGGAC 9 cut(s) 571, 594, 1297, 1560, 1625, 1718, 1847, 1876, 2057
BsmI GAATGC 2 cut(s) 440, 1990
BsnI GGCC 7 cut(s) 1045, 1091, 1127, 1475, 1611, 2049, 2060
Bsp1286I GDGCHC 6 cut(s) 149, 702, 1035, 1409, 2100, 2148
Bsp13I TCCGGA 2 cut(s) 664, 1198
Bsp143I GATC 4 cut(s) 570, 994, 1904, 2183
BspACI CCGC 8 cut(s) 472, 560, 1101, 1289, 1645, 1677, 1762, 2075
BspANI GGCC 7 cut(s) 1045, 1091, 1127, 1475, 1611, 2049, 2060
BspCNI CTCAG 1 cut(s) 1615
BspDI ATCGAT 1 cut(s) 192
BspEI TCCGGA 2 cut(s) 664, 1198
BspHI TCATGA 2 cut(s) 981, 2275
BspLI GGNNCC 7 cut(s) 587, 1178, 1455, 1576, 1599, 1751, 1771
BspMAI CTGCAG 1 cut(s) 187
BspMI ACCTGC 1 cut(s) 1680
BspPI GGATC 3 cut(s) 578, 1912, 2191
BspT107I GGYRCC 1 cut(s) 1597
BsrFI RCCGGY 1 cut(s) 2121
BsrI ACTGG 7 cut(s) 636, 692, 826, 899, 1476, 1578, 1639
BssAI RCCGGY 1 cut(s) 2121
BssECI CCNNGG 3 cut(s) 198, 1431, 1601
BssMI GATC 4 cut(s) 570, 994, 1904, 2183
BssNAI GTATAC 2 cut(s) 799, 1931
BssNI GRCGYC 1 cut(s) 1944
BssSI CACGAG 1 cut(s) 257
BssT1I CCWWGG 1 cut(s) 1431
Bst1107I GTATAC 2 cut(s) 799, 1931
Bst2BI CACGAG 1 cut(s) 257
Bst2UI CCWGG 4 cut(s) 497, 1602, 1911, 1940
Bst6I CTCTTC 1 cut(s) 1874
BstACI GRCGYC 1 cut(s) 1944
BstAPI GCANNNNNTGC 1 cut(s) 1067
BstBAI YACGTR 1 cut(s) 1000
BstC8I GCNNGC 4 cut(s) 108, 1125, 1677, 2084
BstDEI CTNAG 2 cut(s) 1623, 2231
BstEII GGTNACC 1 cut(s) 1309
BstENI CCTNNNNNAGG 1 cut(s) 1605
BstF5I GGATG 3 cut(s) 149, 529, 1680
BstH2I RGCGCY 1 cut(s) 1354
BstHHI GCGC 3 cut(s) 512, 1135, 1353
BstKTI GATC 4 cut(s) 573, 997, 1907, 2186
BstMAI GTCTC 1 cut(s) 2288
BstMBI GATC 4 cut(s) 570, 994, 1904, 2183
BstMCI CGRYCG 1 cut(s) 564
BstMWI GCNNNNNNNGC 6 cut(s) 1067, 1079, 1088, 1286, 2083, 2095
BstNI CCWGG 4 cut(s) 497, 1602, 1911, 1940
BstNSI RCATGY 2 cut(s) 344, 1789
BstPAI GACNNNNGTC 2 cut(s) 297, 1637
BstPI GGTNACC 1 cut(s) 1309
BstSCI CCNGG 6 cut(s) 198, 495, 1280, 1600, 1909, 1938
BstSFI CTRYAG 3 cut(s) 101, 183, 280
BstSLI GKGCMC 3 cut(s) 149, 1409, 2148
BstV1I GCAGC 8 cut(s) 958, 1054, 1057, 1264, 1340, 1977, 1980, 2098
BstX2I RGATCY 1 cut(s) 1904
BstYI RGATCY 1 cut(s) 1904
BstZ17I GTATAC 2 cut(s) 799, 1931
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 7 cut(s) 1045, 1091, 1127, 1475, 1611, 2049, 2060
BsuTUI ATCGAT 1 cut(s) 192
BtgZI GCGATG 2 cut(s) 460, 816
BtsCI GGATG 3 cut(s) 149, 529, 1680
BtsIMutI CAGTG 6 cut(s) 93, 924, 1387, 1469, 2160, 2260
BveI ACCTGC 1 cut(s) 1680
Cac8I GCNNGC 4 cut(s) 108, 1125, 1677, 2084
CaiI CAGNNNCTG 1 cut(s) 1916
CciI TCATGA 2 cut(s) 981, 2275
CfoI GCGC 3 cut(s) 512, 1135, 1353
Cfr10I RCCGGY 1 cut(s) 2121
Cfr13I GGNCC 6 cut(s) 43, 585, 662, 1126, 1574, 1769
ClaI ATCGAT 1 cut(s) 192
CseI GACGC 2 cut(s) 751, 1952
CspAI ACCGGT 1 cut(s) 2121
DdeI CTNAG 2 cut(s) 1623, 2231
DpnI GATC 4 cut(s) 572, 996, 1906, 2185
DpnII GATC 4 cut(s) 570, 994, 1904, 2183
DrdI GACNNNNNNGTC 1 cut(s) 761
DriI GACNNNNNGTC 1 cut(s) 464
DseDI GACNNNNNNGTC 1 cut(s) 761
EaeI YGGCCR 1 cut(s) 2047
Eam1104I CTCTTC 1 cut(s) 1874
Eam1105I GACNNNNNGTC 1 cut(s) 464
EarI CTCTTC 1 cut(s) 1874
Ecl136II GAGCTC 1 cut(s) 2098
Eco130I CCWWGG 1 cut(s) 1431
Eco147I AGGCCT 1 cut(s) 1611
Eco24I GRGCYC 1 cut(s) 2100
Eco47I GGWCC 5 cut(s) 43, 585, 662, 1574, 1769
Eco53kI GAGCTC 1 cut(s) 2098
Eco57I CTGAAG 2 cut(s) 56, 1363
Eco91I GGTNACC 1 cut(s) 1309
EcoICRI GAGCTC 1 cut(s) 2098
EcoNI CCTNNNNNAGG 1 cut(s) 1605
EcoO109I RGGNCCY 1 cut(s) 1126
EcoO65I GGTNACC 1 cut(s) 1309
EcoRII CCWGG 4 cut(s) 495, 1600, 1909, 1938
EcoT14I CCWWGG 1 cut(s) 1431
EcoT22I ATGCAT 1 cut(s) 2227
EcoT38I GRGCYC 1 cut(s) 2100
ErhI CCWWGG 1 cut(s) 1431
FaqI GGGAC 9 cut(s) 571, 594, 1297, 1560, 1625, 1718, 1847, 1876, 2057
FauI CCCGC 3 cut(s) 1652, 1684, 2082
FblI GTMKAC 4 cut(s) 337, 798, 1930, 2010
Fnu4HI GCNGC 8 cut(s) 972, 1068, 1071, 1278, 1354, 1966, 1969, 2087
FokI GGATG 3 cut(s) 136, 536, 1667
FriOI GRGCYC 1 cut(s) 2100
Fsp4HI GCNGC 8 cut(s) 972, 1068, 1071, 1278, 1354, 1966, 1969, 2087
FspBI CTAG 4 cut(s) 35, 759, 1926, 2175
GlaI GCGC 3 cut(s) 511, 1134, 1352
GluI GCNGC 8 cut(s) 972, 1068, 1071, 1278, 1354, 1966, 1969, 2087
GsaI CCCAGC 1 cut(s) 1453
GsuI CTGGAG 3 cut(s) 522, 1656, 1893
HaeII RGCGCY 1 cut(s) 1354
HaeIII GGCC 7 cut(s) 1045, 1091, 1127, 1475, 1611, 2049, 2060
HapII CCGG 7 cut(s) 200, 665, 1199, 1281, 1882, 2036, 2122
HgaI GACGC 2 cut(s) 751, 1952
HhaI GCGC 3 cut(s) 512, 1135, 1353
Hin1I GRCGYC 1 cut(s) 1944
Hin6I GCGC 3 cut(s) 510, 1133, 1351
HinP1I GCGC 3 cut(s) 510, 1133, 1351
HincII GTYRAC 1 cut(s) 338
HindII GTYRAC 1 cut(s) 338
HindIII AAGCTT 1 cut(s) 771
HpaII CCGG 7 cut(s) 200, 665, 1199, 1281, 1882, 2036, 2122
HphI GGTGA 3 cut(s) 428, 771, 1657
Hpy166II GTNNAC 8 cut(s) 68, 338, 799, 1618, 1931, 2011, 2146, 2170
Hpy188I TCNGA 7 cut(s) 54, 75, 215, 433, 1323, 1624, 1918
Hpy8I GTNNAC 8 cut(s) 68, 338, 799, 1618, 1931, 2011, 2146, 2170
Hpy99I CGWCG 2 cut(s) 1841, 2105
HpyAV CCTTC 7 cut(s) 449, 802, 956, 1486, 1727, 1895, 2194
HpyCH4IV ACGT 3 cut(s) 77, 603, 999
HpyF10VI GCNNNNNNNGC 6 cut(s) 1067, 1079, 1088, 1286, 2083, 2095
HpyF3I CTNAG 2 cut(s) 1623, 2231
HpySE526I ACGT 3 cut(s) 77, 603, 999
Hsp92I GRCGYC 1 cut(s) 1944
HspAI GCGC 3 cut(s) 510, 1133, 1351
Kpn2I TCCGGA 2 cut(s) 664, 1198
KpnI GGTACC 1 cut(s) 1601
Kzo9I GATC 4 cut(s) 570, 994, 1904, 2183
LmnI GCTCC 5 cut(s) 64, 215, 385, 705, 2095
Lsp1109I GCAGC 8 cut(s) 958, 1054, 1057, 1264, 1340, 1977, 1980, 2098
LweI GCATC 8 cut(s) 184, 741, 839, 997, 1239, 1524, 1656, 2122
MaeI CTAG 4 cut(s) 35, 759, 1926, 2175
MaeII ACGT 3 cut(s) 77, 603, 999
MaeIII GTNAC 1 cut(s) 1309
MalI GATC 4 cut(s) 572, 996, 1906, 2185
MboI GATC 4 cut(s) 570, 994, 1904, 2183
MboII GAAGA 8 cut(s) 215, 727, 1369, 1642, 1751, 1754, 1861, 2248
MflI RGATCY 1 cut(s) 1904
MhlI GDGCHC 6 cut(s) 149, 702, 1035, 1409, 2100, 2148
MluCI AATT 6 cut(s) 656, 717, 1207, 1266, 1841, 2188
MlyI GAGTC 6 cut(s) 1161, 1357, 1647, 1898, 2006, 2290
MmeI TCCRAC 2 cut(s) 432, 2275
Mph1103I ATGCAT 1 cut(s) 2227
MroI TCCGGA 2 cut(s) 664, 1198
MseI TTAA 5 cut(s) 804, 1206, 1235, 1388, 1758
MslI CAYNNNNRTG 2 cut(s) 788, 1532
MspA1I CMGCKG 2 cut(s) 560, 2089
MspI CCGG 7 cut(s) 200, 665, 1199, 1281, 1882, 2036, 2122
MspR9I CCNGG 6 cut(s) 200, 497, 1282, 1602, 1911, 1940
Mva1269I GAATGC 2 cut(s) 440, 1990
MvaI CCWGG 4 cut(s) 497, 1602, 1911, 1940
MwoI GCNNNNNNNGC 6 cut(s) 1067, 1079, 1088, 1286, 2083, 2095
NciI CCSGG 2 cut(s) 200, 1282
NdeII GATC 4 cut(s) 570, 994, 1904, 2183
NlaIV GGNNCC 7 cut(s) 587, 1178, 1455, 1576, 1599, 1751, 1771
NmeAIII GCCGAG 1 cut(s) 2103
NsiI ATGCAT 1 cut(s) 2227
NspI RCATGY 2 cut(s) 344, 1789
PagI TCATGA 2 cut(s) 981, 2275
PceI AGGCCT 1 cut(s) 1611
PciI ACATGT 1 cut(s) 1785
PctI GAATGC 2 cut(s) 440, 1990
PfeI GAWTC 6 cut(s) 189, 529, 726, 1213, 1222, 2030
PflFI GACNNNGTC 1 cut(s) 1572
PflMI CCANNNNNTGG 2 cut(s) 447, 2056
PinAI ACCGGT 1 cut(s) 2121
PkrI GCNGC 8 cut(s) 973, 1069, 1072, 1279, 1355, 1967, 1970, 2088
PleI GAGTC 6 cut(s) 1160, 1357, 1646, 1897, 2006, 2289
PpsI GAGTC 6 cut(s) 1160, 1357, 1646, 1897, 2006, 2289
Ppu21I YACGTR 1 cut(s) 1000
PscI ACATGT 1 cut(s) 1785
PshAI GACNNNNGTC 2 cut(s) 297, 1637
PshBI ATTAAT 1 cut(s) 1206
Psp124BI GAGCTC 1 cut(s) 2100
Psp6I CCWGG 4 cut(s) 495, 1600, 1909, 1938
PspEI GGTNACC 1 cut(s) 1309
PspFI CCCAGC 1 cut(s) 1449
PspGI CCWGG 4 cut(s) 495, 1600, 1909, 1938
PspN4I GGNNCC 7 cut(s) 587, 1178, 1455, 1576, 1599, 1751, 1771
PspPI GGNCC 6 cut(s) 43, 585, 662, 1126, 1574, 1769
PstI CTGCAG 1 cut(s) 187
PstNI CAGNNNCTG 1 cut(s) 1916
PsuI RGATCY 1 cut(s) 1904
PsyI GACNNNGTC 1 cut(s) 1572
PvuII CAGCTG 1 cut(s) 2089
RseI CAYNNNNRTG 2 cut(s) 788, 1532
SacI GAGCTC 1 cut(s) 2100
SalI GTCGAC 1 cut(s) 336
SaqAI TTAA 5 cut(s) 804, 1206, 1235, 1388, 1758
SatI GCNGC 8 cut(s) 972, 1068, 1071, 1278, 1354, 1966, 1969, 2087
Sau3AI GATC 4 cut(s) 570, 994, 1904, 2183
Sau96I GGNCC 6 cut(s) 43, 585, 662, 1126, 1574, 1769
SbfI CCTGCAGG 1 cut(s) 187
SchI GAGTC 6 cut(s) 1161, 1357, 1647, 1898, 2006, 2290
ScrFI CCNGG 6 cut(s) 200, 497, 1282, 1602, 1911, 1940
SdaI CCTGCAGG 1 cut(s) 187
SduI GDGCHC 6 cut(s) 149, 702, 1035, 1409, 2100, 2148
SfaNI GCATC 8 cut(s) 184, 741, 839, 997, 1239, 1524, 1656, 2122
SfcI CTRYAG 3 cut(s) 101, 183, 280
SinI GGWCC 5 cut(s) 43, 585, 662, 1574, 1769
SmiMI CAYNNNNRTG 2 cut(s) 788, 1532
SmlI CTYRAG 2 cut(s) 1886, 2287
SmoI CTYRAG 2 cut(s) 1886, 2287
Sse8387I CCTGCAGG 1 cut(s) 187
Sse9I AATT 6 cut(s) 656, 717, 1207, 1266, 1841, 2188
SseBI AGGCCT 1 cut(s) 1611
SsiI CCGC 8 cut(s) 472, 560, 1101, 1289, 1645, 1677, 1762, 2075
SspMI CTAG 4 cut(s) 35, 759, 1926, 2175
SstI GAGCTC 1 cut(s) 2100
StuI AGGCCT 1 cut(s) 1611
StyD4I CCNGG 6 cut(s) 198, 495, 1280, 1600, 1909, 1938
StyI CCWWGG 1 cut(s) 1431
TaiI ACGT 3 cut(s) 80, 606, 1002
TaqI TCGA 9 cut(s) 192, 337, 1172, 1246, 1550, 1982, 1997, 2019, 2100
TaqII GACCGA 1 cut(s) 214
TasI AATT 6 cut(s) 656, 717, 1207, 1266, 1841, 2188
TatI WGTACW 3 cut(s) 610, 1423, 1617
TfiI GAWTC 6 cut(s) 189, 529, 726, 1213, 1222, 2030
Tru1I TTAA 5 cut(s) 804, 1206, 1235, 1388, 1758
Tru9I TTAA 5 cut(s) 804, 1206, 1235, 1388, 1758
TscAI CASTG 6 cut(s) 93, 931, 1387, 1476, 2167, 2260
TseI GCWGC 8 cut(s) 971, 1067, 1070, 1277, 1353, 1965, 1968, 2086
TspDTI ATGAA 4 cut(s) 315, 998, 1004, 1101
TspGWI ACGGA 3 cut(s) 876, 881, 1781
TspRI CASTG 6 cut(s) 93, 931, 1387, 1476, 2167, 2260
Tth111I GACNNNGTC 1 cut(s) 1572
Van91I CCANNNNNTGG 2 cut(s) 447, 2056
VneI GTGCAC 1 cut(s) 2144
VpaK11BI GGWCC 5 cut(s) 43, 585, 662, 1574, 1769
VspI ATTAAT 1 cut(s) 1206
XagI CCTNNNNNAGG 1 cut(s) 1605
XapI RAATTY 2 cut(s) 1841, 2188
XceI RCATGY 2 cut(s) 344, 1789
XcmI CCANNNNNNNNNTGG 2 cut(s) 448, 1585
XmiI GTMKAC 4 cut(s) 337, 798, 1930, 2010
XspI CTAG 4 cut(s) 35, 759, 1926, 2175
Zsp2I ATGCAT 1 cut(s) 2227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.