pycom111g01820

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0002827
Physical Location & Seq
Forward (+)
1112821 .. 1114809
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1302 bp
ATGCCTGTTTGTTTTTCTCATCACCTCTTTGTACACAGTATAAAGAAACACTCTTCCTCCATATATATAGTAAAGGATTTGGACCTCCCTCATGTATCCTGCTCTTGCGTTTTGAGTTGCAGTATGTTTCGTGTGGTGGAAATGCAGATGGCTAGATTCGTCGTTGCCGGTTGTTTCGCTATGTGTTTACTGAGTGTGGGAGGGGAAGGGGTGATAGACACATCGTCGCGTTGTGATTTTCCGGCAATTTACAACTTTGGAGACTCCAACTCAGATACTGGTGGTAACTCAGCTTCATTTTACCCCATGGTATCACCCTGTGGTGAAACTTTCTTCCACAGACCCGCCGGAAGGGGTTGTGACGGCCGTCTCATCATAGATTTTATTGCAAAGCATTTGGAGTTGCCATACTTGAGTCCATATTTGGACTCACTAGAGAGCAATTTCAGGCACGGTGCCAATTTTGCTAGCGGCGGATCTACCATCCGGCCTTACAAGGAAGCCATGTTTGAAAATGGTGTGAGCCCTTTTTATCTTGAAATCCAGATTGCTCAATACCAGCAGTTCAAGTCACGTACCACCACTCTTTCCAAACAAGCCAAGAAACAATCCGACAGATTCCCAATAGTCGATGACTTCTCAAGGGCGCTCTACACTTTCGATACAGGACAGAATGATCTAGCTGCTGGTTTTCGGACAAAGTTCAGCAGTGAACAATTTGAATCAGAAATCACAGACATGATTAACCAGCATGCTAATGCCGTACGAAATTTGTATGAACAAGGGGCAAGGACATTCTGGATACACAACACGGGTCCGATTGGCTGCTTGGGAGTGACCTTAAGCTACCTCCATAATCCGAACCCCGGCTTCATTGACAGCCAGGGTTGTGACAAGTTTCAGAACGACATGGCTAGGACGTTCAATAGGCAGCTGGAGCAGAAAGTGATTCAGCTAAGGAAGGAGCTCCCCCTTTCTGCCATAACATATGTCGATATCTTCGCTGCAAAGTATGAACTTCTCAGCAACGCAAAGAAGAACGGATTTTTGGATAAGGCAAGCATCTGCTGTGGATATCATGACGAAAACAACCATGTGTGGTGTGGGAATAAGGGCACCATAACTAATGGGACTCAAGTATATGCTGGTTCATGTAAGGATCCTTCCTTGTATATCAGCTGGGATGGAGTACACTACACCGAGGCTGCGAACCGTTGGATTGCAGATCGAATAGTCCACGGTTCGTTCACAGACCCGCCGGTTTCGATCAAAAGTTCATGTCATAGAACTCAACCTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

434

Amino Acids

48.41

Weight (kDa)

6.59

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 83 - 411 4.3e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 455, 1115
AccII CGCG 1 cut(s) 229
AciI CCGC 4 cut(s) 345, 471, 474, 1256
AclWI GGATC 3 cut(s) 484, 1154, 1167
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 1 cut(s) 769
AdeI CACNNNGTG 1 cut(s) 320
AfaI GTAC 4 cut(s) 33, 577, 765, 1191
AfiI CCNNNNNNNGG 2 cut(s) 351, 866
AflII CTTAAG 1 cut(s) 841
AgsI TTSAA 5 cut(s) 512, 539, 568, 722, 925
AhdI GACNNNNNGTC 1 cut(s) 223
AjnI CCWGG 1 cut(s) 882
AjuI GAANNNNNNNTTGG 2 cut(s) 1031, 1063
AluBI AGCT 7 cut(s) 293, 683, 846, 934, 955, 967, 1179
AluI AGCT 7 cut(s) 293, 683, 846, 934, 955, 967, 1179
Alw21I GWGCWC 1 cut(s) 969
Alw26I GTCTC 2 cut(s) 255, 374
AlwI GGATC 3 cut(s) 484, 1154, 1167
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 2 cut(s) 364, 488
ApeKI GCWGC 5 cut(s) 683, 825, 931, 1004, 1205
ApoI RAATTY 1 cut(s) 769
AspLEI GCGC 1 cut(s) 649
AspS9I GGNCC 2 cut(s) 82, 815
AsuC2I CCSGG 1 cut(s) 867
AsuHPI GGTGA 4 cut(s) 14, 223, 306, 335
AsuNHI GCTAGC 1 cut(s) 467
AvaII GGWCC 2 cut(s) 82, 815
BaeGI GKGCMC 1 cut(s) 1118
BamHI GGATCC 1 cut(s) 1159
BanI GGYRCC 2 cut(s) 455, 1115
BanII GRGCYC 2 cut(s) 527, 969
BarI GAAGNNNNNNTAC 4 cut(s) 277, 309, 1147, 1179
Bbv12I GWGCWC 1 cut(s) 969
BbvI GCAGC 5 cut(s) 670, 812, 943, 991, 1192
BccI CCATC 3 cut(s) 142, 491, 1178
BceAI ACGGC 3 cut(s) 351, 379, 746
BciT130I CCWGG 1 cut(s) 884
BciVI GTATCC 2 cut(s) 106, 795
BcnI CCSGG 1 cut(s) 867
BcoDI GTCTC 2 cut(s) 255, 374
BfaI CTAG 5 cut(s) 153, 434, 468, 680, 915
BfoI RGCGCY 1 cut(s) 650
BfrI CTTAAG 1 cut(s) 841
BfuI GTATCC 2 cut(s) 106, 795
BisI GCNGC 6 cut(s) 472, 684, 826, 932, 1005, 1206
BlsI GCNGC 6 cut(s) 473, 685, 827, 933, 1006, 1207
Bme1390I CCNGG 2 cut(s) 867, 884
Bme18I GGWCC 2 cut(s) 82, 815
BmeRI GACNNNNNGTC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 82, 815
BmiI GGNNCC 4 cut(s) 457, 816, 1117, 1161
BmrFI CCNGG 2 cut(s) 867, 884
BmsI GCATC 1 cut(s) 1071
BmtI GCTAGC 1 cut(s) 471
BoxI GACNNNNGTC 1 cut(s) 366
BpmI CTGGAG 1 cut(s) 956
Bpu10I CCTNAGC 1 cut(s) 956
BpuEI CTTGAG 3 cut(s) 433, 625, 1119
BpuMI CCSGG 1 cut(s) 867
BsaAI YACGTR 1 cut(s) 575
BsaJI CCNNGG 5 cut(s) 306, 865, 883, 1200, 1237
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 2 cut(s) 351, 866
Bse118I RCCGGY 2 cut(s) 167, 1258
Bse1I ACTGG 1 cut(s) 283
BseBI CCWGG 1 cut(s) 884
BseDI CCNNGG 5 cut(s) 306, 865, 883, 1200, 1237
BseGI GGATG 2 cut(s) 483, 1189
BseLI CCNNNNNNNGG 2 cut(s) 351, 866
BseMII CTCAG 5 cut(s) 182, 285, 303, 1036, 1287
BseNI ACTGG 1 cut(s) 283
BseSI GKGCMC 1 cut(s) 1118
BseX3I CGGCCG 1 cut(s) 364
BseXI GCAGC 5 cut(s) 670, 812, 943, 991, 1192
BseYI CCCAGC 1 cut(s) 1179
Bsh1236I CGCG 1 cut(s) 229
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 2 cut(s) 366, 490
BshNI GGYRCC 2 cut(s) 455, 1115
BsiEI CGRYCG 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 969
BsiSI CCGG 6 cut(s) 168, 242, 348, 487, 867, 1259
BsiWI CGTACG 1 cut(s) 763
BslFI GGGAC 1 cut(s) 1144
BslI CCNNNNNNNGG 2 cut(s) 351, 866
BsmAI GTCTC 2 cut(s) 255, 374
BsmBI CGTCTC 1 cut(s) 374
BsmFI GGGAC 1 cut(s) 1144
BsnI GGCC 2 cut(s) 366, 490
Bsp1286I GDGCHC 3 cut(s) 527, 969, 1118
Bsp1407I TGTACA 1 cut(s) 31
Bsp143I GATC 5 cut(s) 476, 676, 1159, 1225, 1266
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 4 cut(s) 345, 471, 474, 1256
BspANI GGCC 2 cut(s) 366, 490
BspCNI CTCAG 5 cut(s) 183, 284, 302, 1035, 1288
BspFNI CGCG 1 cut(s) 229
BspHI TCATGA 1 cut(s) 1078
BspLI GGNNCC 4 cut(s) 457, 816, 1117, 1161
BspOI GCTAGC 1 cut(s) 471
BspPI GGATC 3 cut(s) 484, 1154, 1167
BspT107I GGYRCC 2 cut(s) 455, 1115
BspTI CTTAAG 1 cut(s) 841
BsrFI RCCGGY 2 cut(s) 167, 1258
BsrGI TGTACA 1 cut(s) 31
BsrI ACTGG 1 cut(s) 283
BssAI RCCGGY 2 cut(s) 167, 1258
BssECI CCNNGG 5 cut(s) 306, 865, 883, 1200, 1237
BssMI GATC 5 cut(s) 476, 676, 1159, 1225, 1266
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 884
Bst4CI ACNGT 4 cut(s) 38, 455, 1214, 1241
Bst6I CTCTTC 1 cut(s) 58
BstAFI CTTAAG 1 cut(s) 841
BstAUI TGTACA 1 cut(s) 31
BstBAI YACGTR 1 cut(s) 575
BstC8I GCNNGC 3 cut(s) 469, 753, 1060
BstDEI CTNAG 6 cut(s) 191, 271, 289, 956, 1022, 1296
BstDSI CCRYGG 2 cut(s) 306, 1237
BstF5I GGATG 2 cut(s) 483, 1189
BstFNI CGCG 1 cut(s) 229
BstH2I RGCGCY 1 cut(s) 650
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 5 cut(s) 479, 679, 1162, 1228, 1269
BstMAI GTCTC 2 cut(s) 255, 374
BstMBI GATC 5 cut(s) 476, 676, 1159, 1225, 1266
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 2 cut(s) 464, 937
BstNI CCWGG 1 cut(s) 884
BstNSI RCATGY 1 cut(s) 755
BstPAI GACNNNNGTC 1 cut(s) 366
BstSCI CCNGG 2 cut(s) 865, 882
BstSLI GKGCMC 1 cut(s) 1118
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 5 cut(s) 670, 812, 943, 991, 1192
BstX2I RGATCY 2 cut(s) 476, 1159
BstYI RGATCY 2 cut(s) 476, 1159
BstZI CGGCCG 1 cut(s) 364
BsuI GTATCC 2 cut(s) 106, 795
BsuRI GGCC 2 cut(s) 366, 490
BtgI CCRYGG 2 cut(s) 306, 1237
BtsCI GGATG 2 cut(s) 483, 1189
BtsI GCAGTG 1 cut(s) 715
BtsIMutI CAGTG 1 cut(s) 715
Cac8I GCNNGC 3 cut(s) 469, 753, 1060
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 1 cut(s) 1078
CfoI GCGC 1 cut(s) 649
Cfr10I RCCGGY 2 cut(s) 167, 1258
Cfr13I GGNCC 2 cut(s) 82, 815
Csp6I GTAC 4 cut(s) 32, 576, 764, 1190
CviQI GTAC 4 cut(s) 32, 576, 764, 1190
DdeI CTNAG 6 cut(s) 191, 271, 289, 956, 1022, 1296
DpnI GATC 5 cut(s) 478, 678, 1161, 1227, 1268
DpnII GATC 5 cut(s) 476, 676, 1159, 1225, 1266
DraIII CACNNNGTG 1 cut(s) 320
DriI GACNNNNNGTC 1 cut(s) 223
EaeI YGGCCR 1 cut(s) 364
EagI CGGCCG 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 58
Eam1105I GACNNNNNGTC 1 cut(s) 223
EarI CTCTTC 1 cut(s) 58
EciI GGCGGA 1 cut(s) 489
Ecl136II GAGCTC 1 cut(s) 967
EclXI CGGCCG 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 2 cut(s) 527, 969
Eco32I GATATC 2 cut(s) 997, 1076
Eco47I GGWCC 2 cut(s) 82, 815
Eco52I CGGCCG 1 cut(s) 364
Eco53kI GAGCTC 1 cut(s) 967
EcoICRI GAGCTC 1 cut(s) 967
EcoRII CCWGG 1 cut(s) 882
EcoRV GATATC 2 cut(s) 997, 1076
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 2 cut(s) 527, 969
ErhI CCWWGG 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 374
FaqI GGGAC 1 cut(s) 1144
FauI CCCGC 2 cut(s) 352, 1263
FauNDI CATATG 1 cut(s) 988
Fnu4HI GCNGC 6 cut(s) 472, 684, 826, 932, 1005, 1206
FokI GGATG 2 cut(s) 470, 1196
FriOI GRGCYC 2 cut(s) 527, 969
Fsp4HI GCNGC 6 cut(s) 472, 684, 826, 932, 1005, 1206
FspBI CTAG 5 cut(s) 153, 434, 468, 680, 915
GlaI GCGC 1 cut(s) 648
GluI GCNGC 6 cut(s) 472, 684, 826, 932, 1005, 1206
GsaI CCCAGC 1 cut(s) 1183
GsuI CTGGAG 1 cut(s) 956
HaeII RGCGCY 1 cut(s) 650
HaeIII GGCC 2 cut(s) 366, 490
HapII CCGG 6 cut(s) 168, 242, 348, 487, 867, 1259
HhaI GCGC 1 cut(s) 649
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HinfI GANTC 8 cut(s) 156, 263, 415, 428, 618, 722, 949, 1132
HpaII CCGG 6 cut(s) 168, 242, 348, 487, 867, 1259
HphI GGTGA 4 cut(s) 14, 223, 306, 335
Hpy166II GTNNAC 6 cut(s) 34, 188, 713, 1192, 1237, 1248
Hpy188I TCNGA 7 cut(s) 274, 613, 696, 727, 819, 861, 903
Hpy188III TCNNGA 4 cut(s) 536, 544, 799, 1079
Hpy8I GTNNAC 6 cut(s) 34, 188, 713, 1192, 1237, 1248
Hpy99I CGWCG 2 cut(s) 164, 229
HpyAV CCTTC 4 cut(s) 200, 345, 955, 1173
HpyCH4III ACNGT 4 cut(s) 38, 455, 1214, 1241
HpyCH4IV ACGT 2 cut(s) 574, 920
HpyCH4V TGCA 5 cut(s) 120, 145, 389, 1007, 1223
HpyF10VI GCNNNNNNNGC 2 cut(s) 464, 937
HpyF3I CTNAG 6 cut(s) 191, 271, 289, 956, 1022, 1296
HpySE526I ACGT 2 cut(s) 574, 920
HspAI GCGC 1 cut(s) 647
Kzo9I GATC 5 cut(s) 476, 676, 1159, 1225, 1266
LmnI GCTCC 3 cut(s) 937, 964, 972
Lsp1109I GCAGC 5 cut(s) 670, 812, 943, 991, 1192
LweI GCATC 1 cut(s) 1071
MaeI CTAG 5 cut(s) 153, 434, 468, 680, 915
MaeII ACGT 2 cut(s) 574, 920
MaeIII GTNAC 5 cut(s) 284, 359, 570, 835, 890
MalI GATC 5 cut(s) 478, 678, 1161, 1227, 1268
MboI GATC 5 cut(s) 476, 676, 1159, 1225, 1266
MboII GAAGA 4 cut(s) 45, 325, 991, 1048
MflI RGATCY 2 cut(s) 476, 1159
MhlI GDGCHC 3 cut(s) 527, 969, 1118
MluCI AATT 5 cut(s) 246, 442, 460, 716, 769
MlyI GAGTC 4 cut(s) 257, 422, 424, 1126
MmeI TCCRAC 3 cut(s) 291, 636, 1196
MnlI CCTC 7 cut(s) 35, 67, 95, 99, 194, 860, 1195
MseI TTAA 2 cut(s) 744, 842
MslI CAYNNNNRTG 2 cut(s) 737, 756
MspA1I CMGCKG 2 cut(s) 934, 1179
MspCI CTTAAG 1 cut(s) 841
MspI CCGG 6 cut(s) 168, 242, 348, 487, 867, 1259
MspR9I CCNGG 2 cut(s) 867, 884
MvaI CCWGG 1 cut(s) 884
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 2 cut(s) 464, 937
NciI CCSGG 1 cut(s) 867
NcoI CCATGG 1 cut(s) 306
NdeI CATATG 1 cut(s) 988
NdeII GATC 5 cut(s) 476, 676, 1159, 1225, 1266
NheI GCTAGC 1 cut(s) 467
NlaIV GGNNCC 4 cut(s) 457, 816, 1117, 1161
NmuCI GTSAC 4 cut(s) 359, 570, 835, 890
NspI RCATGY 1 cut(s) 755
PaeI GCATGC 1 cut(s) 755
PagI TCATGA 1 cut(s) 1078
PfeI GAWTC 4 cut(s) 156, 618, 722, 949
Pfl23II CGTACG 1 cut(s) 763
PkrI GCNGC 6 cut(s) 473, 685, 827, 933, 1006, 1207
PleI GAGTC 4 cut(s) 257, 422, 423, 1126
PpsI GAGTC 4 cut(s) 257, 422, 423, 1126
Ppu21I YACGTR 1 cut(s) 575
PshAI GACNNNNGTC 1 cut(s) 366
Psp124BI GAGCTC 1 cut(s) 969
Psp6I CCWGG 1 cut(s) 882
PspFI CCCAGC 1 cut(s) 1179
PspGI CCWGG 1 cut(s) 882
PspLI CGTACG 1 cut(s) 763
PspN4I GGNNCC 4 cut(s) 457, 816, 1117, 1161
PspPI GGNCC 2 cut(s) 82, 815
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 2 cut(s) 476, 1159
PvuII CAGCTG 2 cut(s) 934, 1179
RsaI GTAC 4 cut(s) 33, 577, 765, 1191
RsaNI GTAC 4 cut(s) 32, 576, 764, 1190
RseI CAYNNNNRTG 2 cut(s) 737, 756
SacI GAGCTC 1 cut(s) 969
SaqAI TTAA 2 cut(s) 744, 842
SatI GCNGC 6 cut(s) 472, 684, 826, 932, 1005, 1206
Sau3AI GATC 5 cut(s) 476, 676, 1159, 1225, 1266
Sau96I GGNCC 2 cut(s) 82, 815
SchI GAGTC 4 cut(s) 257, 422, 424, 1126
ScrFI CCNGG 2 cut(s) 867, 884
SduI GDGCHC 3 cut(s) 527, 969, 1118
SfaNI GCATC 1 cut(s) 1071
SinI GGWCC 2 cut(s) 82, 815
SmiMI CAYNNNNRTG 2 cut(s) 737, 756
SmlI CTYRAG 4 cut(s) 412, 640, 841, 1134
SmoI CTYRAG 4 cut(s) 412, 640, 841, 1134
SphI GCATGC 1 cut(s) 755
Sse9I AATT 5 cut(s) 246, 442, 460, 716, 769
SsiI CCGC 4 cut(s) 345, 471, 474, 1256
SspMI CTAG 5 cut(s) 153, 434, 468, 680, 915
SstI GAGCTC 1 cut(s) 969
StyD4I CCNGG 2 cut(s) 865, 882
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 4 cut(s) 38, 455, 1214, 1241
TaiI ACGT 2 cut(s) 577, 923
TaqI TCGA 5 cut(s) 630, 660, 993, 1228, 1265
TasI AATT 5 cut(s) 246, 442, 460, 716, 769
TatI WGTACW 2 cut(s) 31, 1189
TauI GCSGC 1 cut(s) 474
TfiI GAWTC 4 cut(s) 156, 618, 722, 949
Tru1I TTAA 2 cut(s) 744, 842
Tru9I TTAA 2 cut(s) 744, 842
TscAI CASTG 1 cut(s) 715
TseFI GTSAC 4 cut(s) 359, 570, 835, 890
TseI GCWGC 5 cut(s) 683, 825, 931, 1004, 1205
Tsp45I GTSAC 4 cut(s) 359, 570, 835, 890
TspDTI ATGAA 6 cut(s) 285, 792, 862, 1029, 1140, 1266
TspGWI ACGGA 1 cut(s) 1056
TspRI CASTG 1 cut(s) 715
Vha464I CTTAAG 1 cut(s) 841
VpaK11BI GGWCC 2 cut(s) 82, 815
XapI RAATTY 1 cut(s) 769
XceI RCATGY 1 cut(s) 755
XcmI CCANNNNNNNNNTGG 1 cut(s) 1100
XspI CTAG 5 cut(s) 153, 434, 468, 680, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.