pycom11g06300

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002278
Physical Location & Seq
Reverse (-)
4830068 .. 4831042
975 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
ATGGATTTTCTTTCCACTCCCAATGCAATAAGTTTAGCTGTAGTAGTTTTAGCTATACTGGCGATCAGATCCTATGCCAGGAAAAGATTGCAACGCTCTGAGAAGACAAAGAGATACCCTCCTGTTGTCACAACCTTCTTACACACCCTCGTCAACTTCTACAGGTTGCACGATTTTATCACTGAGTTTGCATACAAACACAAAACTTTCAGGGCGCTTAACTTGTTCGTAAATTACGTTGTCACCGCCGATCCTGCAAATATTGAATACATTCTCAAAACAAACTTCGCAAATTATGGCAGGGGGTTGTACAATTACGACATTTTGACAGATGCTTTGGGTAATGGGATCTTCGCTGTGGATGGTGAAAAGTGGATACATCAAAGGAAGTTATCTAGCTACGAAATATCAACCAAAGTAGCTAAAGACTTCAGCAATGGAGTTTTCAAAACCAAAGCAGTAAAGCTTGCTGGGATAATTTCTAAAGCGGCAACCTGCGACCAAGTAATAGAGATCCAAGATTTGATTATGAAAGCGGGGTTAGATTCAGTCGTCAAGATTCTGCTTGGAATGGAAGTAGATACCATGTATGGGACAAATGAAGAAGGCACCCGGTTTGCCCATGCTTTCGATGAAGCAAACAAACTTACCCTGTATCGTTTTGTTGATTTCTCCTGGAAAATCAAACGGTTCTTCAACATTGGGTCAGAAGCAAAGCTGCGGAATGATATTAAACTGATGGATCGATCTTATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

253

Amino Acids

28.84

Weight (kDa)

9.43

Isoelectric Point (pI)

31.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 54 - 232 2.7e-08 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 757
Acc36I ACCTGC 1 cut(s) 503
AccB1I GGYRCC 1 cut(s) 608
AciI CCGC 4 cut(s) 246, 488, 536, 721
AclWI GGATC 5 cut(s) 63, 245, 356, 508, 750
AcuI CTGAAG 1 cut(s) 415
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 2 cut(s) 78, 591
AgsI TTSAA 3 cut(s) 266, 448, 697
AjnI CCWGG 2 cut(s) 77, 674
AluBI AGCT 6 cut(s) 38, 53, 399, 422, 466, 718
AluI AGCT 6 cut(s) 38, 53, 399, 422, 466, 718
AlwI GGATC 5 cut(s) 63, 245, 356, 508, 750
ApeKI GCWGC 1 cut(s) 718
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
Asp700I GAANNNNTTC 1 cut(s) 270
AspLEI GCGC 1 cut(s) 217
AsuC2I CCSGG 1 cut(s) 613
AsuHPI GGTGA 2 cut(s) 235, 377
BanI GGYRCC 1 cut(s) 608
BbsI GAAGAC 1 cut(s) 110
BbvI GCAGC 1 cut(s) 705
BccI CCATC 2 cut(s) 356, 733
BciT130I CCWGG 2 cut(s) 79, 676
BciVI GTATCC 1 cut(s) 369
BcnI CCSGG 1 cut(s) 613
BfaI CTAG 1 cut(s) 396
BfmI CTRYAG 2 cut(s) 39, 160
BfoI RGCGCY 1 cut(s) 218
BfuAI ACCTGC 1 cut(s) 503
BfuI GTATCC 1 cut(s) 369
BisI GCNGC 2 cut(s) 489, 719
BlsI GCNGC 2 cut(s) 490, 720
Bme1390I CCNGG 3 cut(s) 79, 613, 676
BmiI GGNNCC 1 cut(s) 610
BmrFI CCNGG 3 cut(s) 79, 613, 676
BmsI GCATC 1 cut(s) 322
BpiI GAAGAC 1 cut(s) 110
BplI GAGNNNNNCTC 2 cut(s) 103, 135
BpuMI CCSGG 1 cut(s) 613
Bsa29I ATCGAT 1 cut(s) 745
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 591
Bse1I ACTGG 1 cut(s) 63
Bse3DI GCAATG 1 cut(s) 442
BseBI CCWGG 2 cut(s) 79, 676
BseCI ATCGAT 1 cut(s) 745
BseGI GGATG 1 cut(s) 367
BseLI CCNNNNNNNGG 2 cut(s) 78, 591
BseMI GCAATG 1 cut(s) 442
BseMII CTCAG 2 cut(s) 90, 174
BseNI ACTGG 1 cut(s) 63
BseXI GCAGC 1 cut(s) 705
BseYI CCCAGC 1 cut(s) 470
BshNI GGYRCC 1 cut(s) 608
BshVI ATCGAT 1 cut(s) 745
BsiSI CCGG 1 cut(s) 613
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 2 cut(s) 78, 591
BsmFI GGGAC 1 cut(s) 607
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 7 cut(s) 63, 68, 250, 348, 513, 742, 746
BspACI CCGC 4 cut(s) 246, 488, 536, 721
BspCNI CTCAG 2 cut(s) 91, 175
BspDI ATCGAT 1 cut(s) 745
BspLI GGNNCC 1 cut(s) 610
BspMI ACCTGC 1 cut(s) 503
BspPI GGATC 5 cut(s) 63, 245, 356, 508, 750
BspT107I GGYRCC 1 cut(s) 608
BsrDI GCAATG 1 cut(s) 442
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 1 cut(s) 63
BssMI GATC 7 cut(s) 63, 68, 250, 348, 513, 742, 746
Bst2UI CCWGG 2 cut(s) 79, 676
Bst4CI ACNGT 1 cut(s) 690
BstAUI TGTACA 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 468
BstDEI CTNAG 2 cut(s) 99, 183
BstENI CCTNNNNNAGG 1 cut(s) 76
BstF5I GGATG 1 cut(s) 367
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 7 cut(s) 66, 71, 253, 351, 516, 745, 749
BstMBI GATC 7 cut(s) 63, 68, 250, 348, 513, 742, 746
BstMWI GCNNNNNNNGC 2 cut(s) 59, 254
BstNI CCWGG 2 cut(s) 79, 676
BstSCI CCNGG 3 cut(s) 77, 611, 674
BstSFI CTRYAG 2 cut(s) 39, 160
BstV1I GCAGC 1 cut(s) 705
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 3 cut(s) 68, 348, 513
BstYI RGATCY 3 cut(s) 68, 348, 513
Bsu15I ATCGAT 1 cut(s) 745
BsuI GTATCC 1 cut(s) 369
BsuTUI ATCGAT 1 cut(s) 745
BtsCI GGATG 1 cut(s) 367
BtsIMutI CAGTG 1 cut(s) 180
BveI ACCTGC 1 cut(s) 503
Cac8I GCNNGC 1 cut(s) 468
CfoI GCGC 1 cut(s) 217
ClaI ATCGAT 1 cut(s) 745
Csp6I GTAC 1 cut(s) 310
CviAII CATG 2 cut(s) 586, 623
CviJI RGCY 6 cut(s) 38, 53, 399, 422, 466, 718
CviKI_1 RGCY 6 cut(s) 38, 53, 399, 422, 466, 718
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 2 cut(s) 99, 183
DpnI GATC 7 cut(s) 65, 70, 252, 350, 515, 744, 748
DpnII GATC 7 cut(s) 63, 68, 250, 348, 513, 742, 746
Eco57I CTGAAG 1 cut(s) 415
EcoNI CCTNNNNNAGG 1 cut(s) 76
EcoRII CCWGG 2 cut(s) 77, 674
FaeI CATG 2 cut(s) 589, 626
FaiI YATR 9 cut(s) 56, 75, 193, 297, 530, 587, 591, 624, 757
FaqI GGGAC 1 cut(s) 607
FatI CATG 2 cut(s) 585, 622
FauI CCCGC 1 cut(s) 529
Fnu4HI GCNGC 2 cut(s) 489, 719
FokI GGATG 1 cut(s) 374
Fsp4HI GCNGC 2 cut(s) 489, 719
FspBI CTAG 1 cut(s) 396
GlaI GCGC 1 cut(s) 216
GluI GCNGC 2 cut(s) 489, 719
GsaI CCCAGC 1 cut(s) 474
HaeII RGCGCY 1 cut(s) 218
HapII CCGG 1 cut(s) 613
HhaI GCGC 1 cut(s) 217
Hin1II CATG 2 cut(s) 589, 626
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 2 cut(s) 545, 559
HpaII CCGG 1 cut(s) 613
HphI GGTGA 2 cut(s) 235, 377
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 3 cut(s) 68, 100, 709
Hpy188III TCNNGA 1 cut(s) 556
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 2 cut(s) 145, 599
HpyCH4III ACNGT 1 cut(s) 690
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 5 cut(s) 26, 91, 169, 191, 257
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 254
HpyF3I CTNAG 2 cut(s) 99, 183
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 2 cut(s) 589, 626
HspAI GCGC 1 cut(s) 215
Kzo9I GATC 7 cut(s) 63, 68, 250, 348, 513, 742, 746
Lsp1109I GCAGC 1 cut(s) 705
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 396
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 127, 241
MalI GATC 7 cut(s) 65, 70, 252, 350, 515, 744, 748
MboI GATC 7 cut(s) 63, 68, 250, 348, 513, 742, 746
MboII GAAGA 4 cut(s) 115, 343, 614, 685
MflI RGATCY 3 cut(s) 68, 348, 513
MluCI AATT 4 cut(s) 232, 292, 313, 477
MnlI CCTC 2 cut(s) 129, 158
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 2 cut(s) 219, 732
MspI CCGG 1 cut(s) 613
MspR9I CCNGG 3 cut(s) 79, 613, 676
MvaI CCWGG 2 cut(s) 79, 676
MwoI GCNNNNNNNGC 2 cut(s) 59, 254
NciI CCSGG 1 cut(s) 613
NdeII GATC 7 cut(s) 63, 68, 250, 348, 513, 742, 746
NlaIII CATG 2 cut(s) 589, 626
NlaIV GGNNCC 1 cut(s) 610
NmuCI GTSAC 2 cut(s) 127, 241
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 2 cut(s) 545, 559
PfoI TCCNGGA 1 cut(s) 674
PkrI GCNGC 2 cut(s) 490, 720
PsiI TTATAA 1 cut(s) 757
Psp6I CCWGG 2 cut(s) 77, 674
PspFI CCCAGC 1 cut(s) 470
PspGI CCWGG 2 cut(s) 77, 674
PspN4I GGNNCC 1 cut(s) 610
PsuI RGATCY 3 cut(s) 68, 348, 513
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
SaqAI TTAA 2 cut(s) 219, 732
SatI GCNGC 2 cut(s) 489, 719
Sau3AI GATC 7 cut(s) 63, 68, 250, 348, 513, 742, 746
ScrFI CCNGG 3 cut(s) 79, 613, 676
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 2 cut(s) 39, 160
Sse9I AATT 4 cut(s) 232, 292, 313, 477
SsiI CCGC 4 cut(s) 246, 488, 536, 721
SspI AATATT 1 cut(s) 262
SspMI CTAG 1 cut(s) 396
StyD4I CCNGG 3 cut(s) 77, 611, 674
TaaI ACNGT 1 cut(s) 690
TaiI ACGT 1 cut(s) 240
TaqI TCGA 2 cut(s) 630, 745
TasI AATT 4 cut(s) 232, 292, 313, 477
TatI WGTACW 1 cut(s) 309
TauI GCSGC 1 cut(s) 491
TfiI GAWTC 2 cut(s) 545, 559
Tru1I TTAA 2 cut(s) 219, 732
Tru9I TTAA 2 cut(s) 219, 732
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 2 cut(s) 127, 241
TseI GCWGC 1 cut(s) 718
Tsp45I GTSAC 2 cut(s) 127, 241
TspDTI ATGAA 3 cut(s) 545, 615, 648
TspRI CASTG 1 cut(s) 187
XagI CCTNNNNNAGG 1 cut(s) 76
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.