pycom11g18940

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002678
Physical Location & Seq
Reverse (-)
21177295 .. 21179328
2034 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1182 bp
ATGTCCCTCAAACCATCACACAGAAACATCACATGCCAAATTAAGTTTGAGGGAGTTTTTAGACACGATTCATTCATAACCATGGCTAAACATCACACTGATGTTTTGCCAATGGCTTCCTTTGTCGTCGTCTTCTCTCTCTTCACTACTAGTTTCATCGTCGATCTCTCGGAAGGTCAGATGGTTCCGGCCATTTACGTGTTTGGAGACTCGTTAGCGGACGTCGGTAACAACAATTACCTAAAAATTTCTCTAGTCAAGGCAGATTTTCCTCATAATGGGGTTGATTATCCTGACAAAGTAGCAACGGGGAGGTTTGGTAATGGCAAGAATTCTGCAGACTTTCTTGGGTTACCACCGTCGACACCATATCTGGCACTTGTATCCAAGTCCAACAGGCGAAAAGTGTCCTTTGTCGATGGAGCTAGCTTCGCCTCCGGAGGAGCTGGAATCTTCAATGGCTCGGATGAACAATTTGTAAGTCGTCAGTCAATACCTTTGTCATACCAAGTAAACTACTACTCAGAAGTACATCAAAACATAGGGTTACAGTTAGGATCCGAGGGCACCAAAGACCATCTTTCAAAATCTCTGTTTCTTATTGTGATTGGAAGCAATGACATCTTCGACTACATTAAAAAATCCAAGCTCCAAAATGAATACACACCGCATCAGTACGCGGATTTAATGGCGTCGAATCTAAAGGAACAATTACAGAGAATACATGCTCTGGGTGCCCAAAAATTTGTAGTTGTTGGGACTGGGCCGGTTGGATGCTGTCCGTCGCAAAGGGATTCAAATACTGAAGAGTGCAATGATGGCGCAAACTCATTGTCTGTCATGTACAATGAAGCCCTTAAGTCCATGTTGCAGCAATTGAAACCGGCATTGGGCATAAGCTACACCCTCTTCGACACTTACAGCGTCTTGACTAACATTGTACAAAACGCAGCTTCTTACGGATTTACAGAGGTTAAAACTGCTTGCTGTGGGATTGGGCAGCTTAACGCCAAGGGTCCTTGCCTTCCAGTTGCATCCTTGTGCTCCAACAGAAGGGACCATGTCTTCTGGGATTTTTACCATCCAACACAGGCAACTCATGGCATCATAGTGGATAAACTTTTCGATGGCCCTTCAGAATACTCATCCCCAATGACTGTGAAGGAGTTAATTGCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

394

Amino Acids

43.07

Weight (kDa)

6.45

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 65 - 373 4.4e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 225
AccB1I GGYRCC 2 cut(s) 566, 734
AccI GTMKAC 1 cut(s) 362
AccII CGCG 1 cut(s) 680
AccIII TCCGGA 1 cut(s) 437
AciI CCGC 3 cut(s) 218, 668, 680
AclWI GGATC 2 cut(s) 552, 565
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 3 cut(s) 246, 331, 743
AcuI CTGAAG 2 cut(s) 825, 1119
AcyI GRCGYC 2 cut(s) 222, 692
AfaI GTAC 4 cut(s) 531, 677, 845, 942
AfiI CCNNNNNNNGG 3 cut(s) 278, 890, 1053
AflII CTTAAG 1 cut(s) 857
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 4 cut(s) 457, 585, 798, 880
AhlI ACTAGT 1 cut(s) 149
AjuI GAANNNNNNNTTGG 2 cut(s) 872, 904
AluBI AGCT 7 cut(s) 425, 429, 446, 649, 900, 953, 1003
AluI AGCT 7 cut(s) 425, 429, 446, 649, 900, 953, 1003
Alw21I GWGCWC 1 cut(s) 1046
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 2 cut(s) 552, 565
Aor13HI TCCGGA 1 cut(s) 437
AoxI GGCC 3 cut(s) 189, 764, 1129
ApeKI GCWGC 3 cut(s) 871, 950, 1000
ApoI RAATTY 3 cut(s) 246, 331, 743
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
AspLEI GCGC 1 cut(s) 824
AspS9I GGNCC 4 cut(s) 764, 1016, 1057, 1130
AsuNHI GCTAGC 1 cut(s) 425
AvaII GGWCC 2 cut(s) 1016, 1057
BaeGI GKGCMC 2 cut(s) 569, 739
BamHI GGATCC 1 cut(s) 557
BanI GGYRCC 2 cut(s) 566, 734
BbsI GAAGAC 2 cut(s) 124, 1057
Bbv12I GWGCWC 1 cut(s) 1046
BbvI GCAGC 3 cut(s) 883, 962, 1012
BccI CCATC 7 cut(s) 22, 175, 413, 585, 812, 1089, 1121
BciVI GTATCC 1 cut(s) 394
BcoDI GTCTC 1 cut(s) 201
BcuI ACTAGT 1 cut(s) 149
BfaI CTAG 3 cut(s) 150, 254, 426
BfmI CTRYAG 1 cut(s) 336
BfrI CTTAAG 1 cut(s) 857
BfuI GTATCC 1 cut(s) 394
BisI GCNGC 3 cut(s) 872, 951, 1001
BlsI GCNGC 3 cut(s) 873, 952, 1002
Bme18I GGWCC 2 cut(s) 1016, 1057
BmgT120I GGNCC 4 cut(s) 764, 1016, 1057, 1130
BmiI GGNNCC 6 cut(s) 186, 559, 568, 736, 1017, 1058
BmrI ACTGGG 1 cut(s) 771
BmsI GCATC 4 cut(s) 679, 764, 1043, 1113
BmtI GCTAGC 1 cut(s) 429
BmuI ACTGGG 1 cut(s) 771
BpiI GAAGAC 2 cut(s) 124, 1057
BsaAI YACGTR 1 cut(s) 199
BsaHI GRCGYC 2 cut(s) 222, 692
BsaJI CCNNGG 3 cut(s) 81, 561, 1011
BsaWI WCCGGW 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 890, 1053
Bse118I RCCGGY 2 cut(s) 766, 883
Bse1I ACTGG 2 cut(s) 766, 1028
Bse3DI GCAATG 2 cut(s) 622, 820
BseAI TCCGGA 1 cut(s) 437
BseDI CCNNGG 3 cut(s) 81, 561, 1011
BseGI GGATG 5 cut(s) 472, 779, 1034, 1081, 1145
BseLI CCNNNNNNNGG 3 cut(s) 278, 890, 1053
BseMI GCAATG 2 cut(s) 622, 820
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 2 cut(s) 766, 1028
BseRI GAGGAG 1 cut(s) 456
BseSI GKGCMC 2 cut(s) 569, 739
BseXI GCAGC 3 cut(s) 883, 962, 1012
Bsh1236I CGCG 1 cut(s) 680
BshFI GGCC 3 cut(s) 191, 766, 1131
BshNI GGYRCC 2 cut(s) 566, 734
BsiHKAI GWGCWC 1 cut(s) 1046
BsiSI CCGG 4 cut(s) 188, 438, 767, 884
BslFI GGGAC 2 cut(s) 772, 1070
BslI CCNNNNNNNGG 3 cut(s) 278, 890, 1053
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 2 cut(s) 772, 1070
BsnI GGCC 3 cut(s) 191, 766, 1131
Bsp1286I GDGCHC 3 cut(s) 569, 739, 1046
Bsp13I TCCGGA 1 cut(s) 437
Bsp1407I TGTACA 2 cut(s) 843, 940
Bsp143I GATC 2 cut(s) 163, 557
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 3 cut(s) 218, 668, 680
BspANI GGCC 3 cut(s) 191, 766, 1131
BspCNI CTCAG 1 cut(s) 536
BspEI TCCGGA 1 cut(s) 437
BspFNI CGCG 1 cut(s) 680
BspLI GGNNCC 6 cut(s) 186, 559, 568, 736, 1017, 1058
BspMAI CTGCAG 1 cut(s) 340
BspOI GCTAGC 1 cut(s) 429
BspPI GGATC 2 cut(s) 552, 565
BspT107I GGYRCC 2 cut(s) 566, 734
BspTI CTTAAG 1 cut(s) 857
BsrDI GCAATG 2 cut(s) 622, 820
BsrFI RCCGGY 2 cut(s) 766, 883
BsrGI TGTACA 2 cut(s) 843, 940
BsrI ACTGG 2 cut(s) 766, 1028
BssAI RCCGGY 2 cut(s) 766, 883
BssECI CCNNGG 3 cut(s) 81, 561, 1011
BssMI GATC 2 cut(s) 163, 557
BssNI GRCGYC 2 cut(s) 222, 692
BssT1I CCWWGG 2 cut(s) 81, 1011
Bst4CI ACNGT 3 cut(s) 360, 552, 1159
Bst6I CTCTTC 3 cut(s) 146, 801, 914
BstACI GRCGYC 2 cut(s) 222, 692
BstAFI CTTAAG 1 cut(s) 857
BstAUI TGTACA 2 cut(s) 843, 940
BstBAI YACGTR 1 cut(s) 199
BstC8I GCNNGC 2 cut(s) 427, 985
BstDEI CTNAG 1 cut(s) 523
BstDSI CCRYGG 1 cut(s) 81
BstEII GGTNACC 1 cut(s) 351
BstF5I GGATG 5 cut(s) 472, 779, 1034, 1081, 1145
BstFNI CGCG 1 cut(s) 680
BstHHI GCGC 1 cut(s) 824
BstKTI GATC 2 cut(s) 166, 560
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 2 cut(s) 163, 557
BstMWI GCNNNNNNNGC 3 cut(s) 431, 734, 819
BstNSI RCATGY 2 cut(s) 36, 728
BstPI GGTNACC 1 cut(s) 351
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 2 cut(s) 569, 739
BstUI CGCG 1 cut(s) 680
BstV1I GCAGC 3 cut(s) 883, 962, 1012
BstV2I GAAGAC 2 cut(s) 124, 1057
BstX2I RGATCY 1 cut(s) 557
BstYI RGATCY 1 cut(s) 557
BsuI GTATCC 1 cut(s) 394
BsuRI GGCC 3 cut(s) 191, 766, 1131
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 5 cut(s) 472, 779, 1034, 1081, 1145
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 2 cut(s) 427, 985
CfoI GCGC 1 cut(s) 824
Cfr10I RCCGGY 2 cut(s) 766, 883
Cfr13I GGNCC 4 cut(s) 764, 1016, 1057, 1130
CseI GACGC 2 cut(s) 681, 913
Csp6I GTAC 4 cut(s) 530, 676, 844, 941
CviAII CATG 7 cut(s) 33, 82, 725, 841, 865, 1061, 1100
CviQI GTAC 4 cut(s) 530, 676, 844, 941
DdeI CTNAG 1 cut(s) 523
DpnI GATC 2 cut(s) 165, 559
DpnII GATC 2 cut(s) 163, 557
EaeI YGGCCR 1 cut(s) 189
Eam1104I CTCTTC 3 cut(s) 146, 801, 914
EarI CTCTTC 3 cut(s) 146, 801, 914
Eco130I CCWWGG 2 cut(s) 81, 1011
Eco47I GGWCC 2 cut(s) 1016, 1057
Eco57I CTGAAG 2 cut(s) 825, 1119
Eco91I GGTNACC 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 1016
EcoO65I GGTNACC 1 cut(s) 351
EcoRI GAATTC 1 cut(s) 331
EcoT14I CCWWGG 2 cut(s) 81, 1011
ErhI CCWWGG 2 cut(s) 81, 1011
FaeI CATG 7 cut(s) 36, 85, 728, 844, 868, 1064, 1103
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FaqI GGGAC 2 cut(s) 772, 1070
FatI CATG 7 cut(s) 32, 81, 724, 840, 864, 1060, 1099
FblI GTMKAC 1 cut(s) 362
Fnu4HI GCNGC 3 cut(s) 872, 951, 1001
FokI GGATG 5 cut(s) 479, 786, 1021, 1068, 1132
Fsp4HI GCNGC 3 cut(s) 872, 951, 1001
FspBI CTAG 3 cut(s) 150, 254, 426
GlaI GCGC 1 cut(s) 823
GluI GCNGC 3 cut(s) 872, 951, 1001
HaeIII GGCC 3 cut(s) 191, 766, 1131
HapII CCGG 4 cut(s) 188, 438, 767, 884
HgaI GACGC 2 cut(s) 681, 913
HhaI GCGC 1 cut(s) 824
Hin1I GRCGYC 2 cut(s) 222, 692
Hin1II CATG 7 cut(s) 36, 85, 728, 844, 868, 1064, 1103
Hin6I GCGC 1 cut(s) 822
HinP1I GCGC 1 cut(s) 822
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
HinfI GANTC 5 cut(s) 68, 209, 450, 697, 794
HpaII CCGG 4 cut(s) 188, 438, 767, 884
Hpy166II GTNNAC 2 cut(s) 363, 514
Hpy188I TCNGA 6 cut(s) 172, 180, 466, 526, 562, 1138
Hpy188III TCNNGA 3 cut(s) 293, 438, 928
Hpy8I GTNNAC 2 cut(s) 363, 514
Hpy99I CGWCG 6 cut(s) 131, 164, 227, 364, 697, 787
HpyAV CCTTC 5 cut(s) 167, 1034, 1047, 1143, 1156
HpyCH4III ACNGT 3 cut(s) 360, 552, 1159
HpyCH4IV ACGT 2 cut(s) 198, 222
HpyCH4V TGCA 4 cut(s) 338, 813, 871, 1034
HpyF10VI GCNNNNNNNGC 3 cut(s) 431, 734, 819
HpyF3I CTNAG 1 cut(s) 523
HpySE526I ACGT 2 cut(s) 198, 222
Hsp92I GRCGYC 2 cut(s) 222, 692
Hsp92II CATG 7 cut(s) 36, 85, 728, 844, 868, 1064, 1103
HspAI GCGC 1 cut(s) 822
Kpn2I TCCGGA 1 cut(s) 437
Kzo9I GATC 2 cut(s) 163, 557
LmnI GCTCC 4 cut(s) 422, 443, 654, 1049
Lsp1109I GCAGC 3 cut(s) 883, 962, 1012
LweI GCATC 4 cut(s) 679, 764, 1043, 1113
MaeI CTAG 3 cut(s) 150, 254, 426
MaeII ACGT 2 cut(s) 198, 222
MaeIII GTNAC 3 cut(s) 227, 351, 546
MalI GATC 2 cut(s) 165, 559
MboI GATC 2 cut(s) 163, 557
MboII GAAGA 7 cut(s) 124, 133, 445, 616, 818, 901, 1057
MfeI CAATTG 1 cut(s) 875
MflI RGATCY 1 cut(s) 557
MhlI GDGCHC 3 cut(s) 569, 739, 1046
MluCI AATT 9 cut(s) 39, 235, 246, 331, 473, 710, 743, 875, 1170
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 4 cut(s) 417, 751, 1071, 1109
MnlI CCTC 9 cut(s) 17, 43, 282, 306, 434, 445, 556, 917, 964
MroI TCCGGA 1 cut(s) 437
MseI TTAA 7 cut(s) 42, 636, 686, 858, 975, 1005, 1169
MslI CAYNNNNRTG 5 cut(s) 80, 99, 197, 1039, 1109
MspCI CTTAAG 1 cut(s) 857
MspI CCGG 4 cut(s) 188, 438, 767, 884
MunI CAATTG 1 cut(s) 875
MvnI CGCG 1 cut(s) 680
MwoI GCNNNNNNNGC 3 cut(s) 431, 734, 819
NcoI CCATGG 1 cut(s) 81
NdeII GATC 2 cut(s) 163, 557
NheI GCTAGC 1 cut(s) 425
NlaIII CATG 7 cut(s) 36, 85, 728, 844, 868, 1064, 1103
NlaIV GGNNCC 6 cut(s) 186, 559, 568, 736, 1017, 1058
NspI RCATGY 2 cut(s) 36, 728
PfeI GAWTC 4 cut(s) 68, 450, 697, 794
PflFI GACNNNGTC 1 cut(s) 1061
PkrI GCNGC 3 cut(s) 873, 952, 1002
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
Ppu21I YACGTR 1 cut(s) 199
PpuMI RGGWCCY 1 cut(s) 1016
Psp5II RGGWCCY 1 cut(s) 1016
PspEI GGTNACC 1 cut(s) 351
PspN4I GGNNCC 6 cut(s) 186, 559, 568, 736, 1017, 1058
PspPI GGNCC 4 cut(s) 764, 1016, 1057, 1130
PspPPI RGGWCCY 1 cut(s) 1016
PstI CTGCAG 1 cut(s) 340
PsuI RGATCY 1 cut(s) 557
PsyI GACNNNGTC 1 cut(s) 1061
RsaI GTAC 4 cut(s) 531, 677, 845, 942
RsaNI GTAC 4 cut(s) 530, 676, 844, 941
RseI CAYNNNNRTG 5 cut(s) 80, 99, 197, 1039, 1109
SalI GTCGAC 1 cut(s) 361
SaqAI TTAA 7 cut(s) 42, 636, 686, 858, 975, 1005, 1169
SatI GCNGC 3 cut(s) 872, 951, 1001
Sau3AI GATC 2 cut(s) 163, 557
Sau96I GGNCC 4 cut(s) 764, 1016, 1057, 1130
SchI GAGTC 1 cut(s) 203
SduI GDGCHC 3 cut(s) 569, 739, 1046
SfaNI GCATC 4 cut(s) 679, 764, 1043, 1113
SfcI CTRYAG 1 cut(s) 336
SinI GGWCC 2 cut(s) 1016, 1057
SmiMI CAYNNNNRTG 5 cut(s) 80, 99, 197, 1039, 1109
SmlI CTYRAG 1 cut(s) 857
SmoI CTYRAG 1 cut(s) 857
SpeI ACTAGT 1 cut(s) 149
Sse9I AATT 9 cut(s) 39, 235, 246, 331, 473, 710, 743, 875, 1170
SsiI CCGC 3 cut(s) 218, 668, 680
SspMI CTAG 3 cut(s) 150, 254, 426
StyI CCWWGG 2 cut(s) 81, 1011
TaaI ACNGT 3 cut(s) 360, 552, 1159
TaiI ACGT 2 cut(s) 201, 225
TaqI TCGA 7 cut(s) 162, 362, 417, 627, 695, 912, 1125
TasI AATT 9 cut(s) 39, 235, 246, 331, 473, 710, 743, 875, 1170
TatI WGTACW 3 cut(s) 529, 843, 940
TfiI GAWTC 4 cut(s) 68, 450, 697, 794
Tru1I TTAA 7 cut(s) 42, 636, 686, 858, 975, 1005, 1169
Tru9I TTAA 7 cut(s) 42, 636, 686, 858, 975, 1005, 1169
TscAI CASTG 1 cut(s) 103
TseI GCWGC 3 cut(s) 871, 950, 1000
TspDTI ATGAA 6 cut(s) 60, 64, 145, 483, 672, 864
TspGWI ACGGA 2 cut(s) 771, 975
TspRI CASTG 1 cut(s) 103
Tth111I GACNNNGTC 1 cut(s) 1061
Vha464I CTTAAG 1 cut(s) 857
VpaK11BI GGWCC 2 cut(s) 1016, 1057
XapI RAATTY 3 cut(s) 246, 331, 743
XceI RCATGY 2 cut(s) 36, 728
XmiI GTMKAC 1 cut(s) 362
XspI CTAG 3 cut(s) 150, 254, 426
ZraI GACGTC 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.