pycom13g15740
ERF Family

Ribosomal protein L34e superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002458
Physical Location & Seq
Reverse (-)
11479999 .. 11482540
2542 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g15740.2

Sequence Viewer

Length: 714 bp
ATGCGCCATTTTCTGTTCGCCACCACCACCATCATGCCCTCCTCATCCTCCTCCTTCTCAGCCGCCGCCACGTCATCCTCCTCCTTCTCCCCAGCCCCCAAACCCAAATCCTCAAAACCTAACTGTAACTCAAACCCTAATTGCACCCTCCTCTGCAAGCACTCGCCCTCGGCGACCCTCGACCTCCTTATCCTCATCCTCGTCCTCTTCTCCGGGACCTTCCTCATCACCTCCTACTTCTCCTACATCTTCAACTCCCTCTCGCTTCTCGTCTCTCACTCCAACCTCCACCTCCTCCTCCACCACATTCCCGTCCCCTACGTCGTCGGCTTCGTCGCCTTCTTCGCCGCCGCGCTGTTCGTAGTCGAGTTCTGCTGCGGCATTAGATCGAAGAAGTGCGATAGGCCTGGGTGCAAGGGCTTGAAGAAGGCTATGGAATTCGATTTGCAATTGCAATCGGAGGAGTGTGTCAAGTCCGGGTCTAAAGATATCGATAGGCTGCCGTGGAAGGGCGGCAGCGAGGGCAATCCCGATTACGAGTGTCTCAGGACTGAGCTGAGGCGGATGGCGCCGACCAATGGCCGTGCCGTTCTGCTGTTTCGGGCTCGATGCGGATGCCCGGTGGCCAAGCTCGAAGGCTGGGGTCCCAAGAGAAAACGTCGGCATAAGAAGGCTCTGGCCGGTATGGCTTTTAATGGAGGAGATCATCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

26.04

Weight (kDa)

9.61

Isoelectric Point (pI)

57.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 568
AccII CGCG 1 cut(s) 353
AciI CCGC 8 cut(s) 63, 66, 348, 351, 378, 513, 562, 612
AcoI YGGCCR 3 cut(s) 580, 624, 678
AcsI RAATTY 1 cut(s) 437
AcyI GRCGYC 1 cut(s) 569
AfiI CCNNNNNNNGG 2 cut(s) 509, 578
AgsI TTSAA 2 cut(s) 253, 424
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 1 cut(s) 406
AluBI AGCT 2 cut(s) 556, 631
AluI AGCT 2 cut(s) 556, 631
Alw26I GTCTC 2 cut(s) 277, 548
AoxI GGCC 4 cut(s) 404, 580, 624, 678
ApeKI GCWGC 3 cut(s) 375, 499, 516
ApoI RAATTY 1 cut(s) 437
AspLEI GCGC 3 cut(s) 6, 355, 571
AspS9I GGNCC 2 cut(s) 216, 644
AsuC2I CCSGG 3 cut(s) 214, 478, 620
AsuHPI GGTGA 1 cut(s) 220
AvaII GGWCC 2 cut(s) 216, 644
BalI TGGCCA 1 cut(s) 626
BanI GGYRCC 1 cut(s) 568
BanII GRGCYC 1 cut(s) 607
BbvCI CCTCAGC 1 cut(s) 557
BbvI GCAGC 3 cut(s) 362, 486, 528
BccI CCATC 2 cut(s) 38, 559
BceAI ACGGC 3 cut(s) 487, 567, 572
BcgI CGANNNNNNTGC 2 cut(s) 597, 631
BciT130I CCWGG 1 cut(s) 408
BcnI CCSGG 3 cut(s) 214, 478, 620
BcoDI GTCTC 2 cut(s) 277, 548
BfoI RGCGCY 1 cut(s) 572
BglI GCCNNNNNGGC 1 cut(s) 686
BisI GCNGC 9 cut(s) 63, 66, 348, 351, 376, 379, 500, 514, 517
BlsI GCNGC 9 cut(s) 64, 67, 349, 352, 377, 380, 501, 515, 518
Bme1390I CCNGG 4 cut(s) 214, 408, 478, 620
Bme18I GGWCC 2 cut(s) 216, 644
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 2 cut(s) 216, 644
BmiI GGNNCC 4 cut(s) 217, 570, 645, 646
BmrFI CCNGG 4 cut(s) 214, 408, 478, 620
BmsI GCATC 2 cut(s) 599, 605
Bpu10I CCTNAGC 1 cut(s) 557
BpuMI CCSGG 3 cut(s) 214, 478, 620
Bsa29I ATCGAT 1 cut(s) 492
BsaHI GRCGYC 1 cut(s) 569
BsaJI CCNNGG 3 cut(s) 168, 407, 503
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 578
Bse118I RCCGGY 1 cut(s) 680
BseBI CCWGG 1 cut(s) 408
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 3 cut(s) 168, 407, 503
BseGI GGATG 5 cut(s) 44, 74, 195, 570, 620
BseLI CCNNNNNNNGG 2 cut(s) 509, 578
BseMII CTCAG 4 cut(s) 72, 543, 548, 559
BseRI GAGGAG 8 cut(s) 31, 40, 70, 140, 284, 287, 476, 714
BseXI GCAGC 3 cut(s) 362, 486, 528
BseYI CCCAGC 2 cut(s) 91, 639
Bsh1236I CGCG 1 cut(s) 353
BshFI GGCC 4 cut(s) 406, 582, 626, 680
BshNI GGYRCC 1 cut(s) 568
BshVI ATCGAT 1 cut(s) 492
BsiSI CCGG 4 cut(s) 213, 477, 620, 681
BslFI GGGAC 3 cut(s) 229, 299, 630
BslI CCNNNNNNNGG 2 cut(s) 509, 578
BsmAI GTCTC 2 cut(s) 277, 548
BsmBI CGTCTC 1 cut(s) 277
BsmFI GGGAC 3 cut(s) 229, 299, 630
BsnI GGCC 4 cut(s) 406, 582, 626, 680
Bsp1286I GDGCHC 1 cut(s) 607
Bsp143I GATC 2 cut(s) 386, 703
BspACI CCGC 8 cut(s) 63, 66, 348, 351, 378, 513, 562, 612
BspANI GGCC 4 cut(s) 406, 582, 626, 680
BspCNI CTCAG 4 cut(s) 71, 544, 549, 558
BspDI ATCGAT 1 cut(s) 492
BspFNI CGCG 1 cut(s) 353
BspLI GGNNCC 4 cut(s) 217, 570, 645, 646
BspT107I GGYRCC 1 cut(s) 568
BsrFI RCCGGY 1 cut(s) 680
BssAI RCCGGY 1 cut(s) 680
BssECI CCNNGG 3 cut(s) 168, 407, 503
BssMI GATC 2 cut(s) 386, 703
BssNI GRCGYC 1 cut(s) 569
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 1 cut(s) 125
Bst6I CTCTTC 1 cut(s) 212
BstACI GRCGYC 1 cut(s) 569
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 4 cut(s) 58, 545, 552, 557
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 5 cut(s) 44, 74, 195, 570, 620
BstFNI CGCG 1 cut(s) 353
BstH2I RGCGCY 1 cut(s) 572
BstHHI GCGC 3 cut(s) 6, 355, 571
BstKTI GATC 2 cut(s) 389, 706
BstMAI GTCTC 2 cut(s) 277, 548
BstMBI GATC 2 cut(s) 386, 703
BstMWI GCNNNNNNNGC 4 cut(s) 344, 522, 568, 686
BstNI CCWGG 1 cut(s) 408
BstSCI CCNGG 4 cut(s) 212, 406, 476, 618
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 3 cut(s) 362, 486, 528
Bsu15I ATCGAT 1 cut(s) 492
BsuRI GGCC 4 cut(s) 406, 582, 626, 680
BsuTUI ATCGAT 1 cut(s) 492
BtgI CCRYGG 1 cut(s) 503
BtgZI GCGATG 1 cut(s) 692
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 5 cut(s) 44, 74, 195, 570, 620
Cac8I GCNNGC 1 cut(s) 158
CfoI GCGC 3 cut(s) 6, 355, 571
Cfr10I RCCGGY 1 cut(s) 680
Cfr13I GGNCC 2 cut(s) 216, 644
ClaI ATCGAT 1 cut(s) 492
CviAII CATG 1 cut(s) 34
DdeI CTNAG 4 cut(s) 58, 545, 552, 557
DinI GGCGCC 1 cut(s) 570
DpnI GATC 2 cut(s) 388, 705
DpnII GATC 2 cut(s) 386, 703
EaeI YGGCCR 3 cut(s) 580, 624, 678
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
EciI GGCGGA 1 cut(s) 577
Eco147I AGGCCT 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 607
Eco32I GATATC 1 cut(s) 490
Eco47I GGWCC 2 cut(s) 216, 644
EcoO109I RGGNCCY 2 cut(s) 216, 644
EcoRI GAATTC 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 406
EcoRV GATATC 1 cut(s) 490
EcoT38I GRGCYC 1 cut(s) 607
EgeI GGCGCC 1 cut(s) 570
EheI GGCGCC 1 cut(s) 570
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 1 cut(s) 37
FaiI YATR 4 cut(s) 35, 434, 666, 686
FaqI GGGAC 3 cut(s) 229, 299, 630
FatI CATG 1 cut(s) 33
Fnu4HI GCNGC 9 cut(s) 63, 66, 348, 351, 376, 379, 500, 514, 517
FokI GGATG 5 cut(s) 31, 61, 182, 577, 627
FriOI GRGCYC 1 cut(s) 607
Fsp4HI GCNGC 9 cut(s) 63, 66, 348, 351, 376, 379, 500, 514, 517
GlaI GCGC 3 cut(s) 5, 354, 570
GluI GCNGC 9 cut(s) 63, 66, 348, 351, 376, 379, 500, 514, 517
GsaI CCCAGC 2 cut(s) 95, 643
HaeII RGCGCY 1 cut(s) 572
HaeIII GGCC 4 cut(s) 406, 582, 626, 680
HapII CCGG 4 cut(s) 213, 477, 620, 681
HhaI GCGC 3 cut(s) 6, 355, 571
Hin1I GRCGYC 1 cut(s) 569
Hin1II CATG 1 cut(s) 37
Hin6I GCGC 3 cut(s) 4, 353, 569
HinP1I GCGC 3 cut(s) 4, 353, 569
HpaII CCGG 4 cut(s) 213, 477, 620, 681
HphI GGTGA 1 cut(s) 220
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 2 cut(s) 530, 547
Hpy99I CGWCG 4 cut(s) 326, 329, 338, 663
HpyAV CCTTC 8 cut(s) 64, 94, 229, 349, 421, 502, 629, 664
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 3 cut(s) 71, 321, 658
HpyCH4V TGCA 5 cut(s) 144, 156, 414, 448, 454
HpyF10VI GCNNNNNNNGC 4 cut(s) 344, 522, 568, 686
HpyF3I CTNAG 4 cut(s) 58, 545, 552, 557
HpySE526I ACGT 3 cut(s) 71, 321, 658
Hsp92I GRCGYC 1 cut(s) 569
Hsp92II CATG 1 cut(s) 37
HspAI GCGC 3 cut(s) 4, 353, 569
KasI GGCGCC 1 cut(s) 568
KflI GGGWCCC 1 cut(s) 644
Kzo9I GATC 2 cut(s) 386, 703
Lsp1109I GCAGC 3 cut(s) 362, 486, 528
LweI GCATC 2 cut(s) 599, 605
MaeII ACGT 3 cut(s) 71, 321, 658
MaeIII GTNAC 1 cut(s) 125
MalI GATC 2 cut(s) 388, 705
MboI GATC 2 cut(s) 386, 703
MboII GAAGA 5 cut(s) 199, 241, 334, 403, 436
MfeI CAATTG 1 cut(s) 449
MhlI GDGCHC 1 cut(s) 607
MlsI TGGCCA 1 cut(s) 626
MluCI AATT 3 cut(s) 139, 437, 449
MluNI TGGCCA 1 cut(s) 626
Mly113I GGCGCC 1 cut(s) 569
MmeI TCCRAC 1 cut(s) 306
Mox20I TGGCCA 1 cut(s) 626
MscI TGGCCA 1 cut(s) 626
MseI TTAA 1 cut(s) 693
MslI CAYNNNNRTG 1 cut(s) 32
Msp20I TGGCCA 1 cut(s) 626
MspI CCGG 4 cut(s) 213, 477, 620, 681
MspR9I CCNGG 4 cut(s) 214, 408, 478, 620
MunI CAATTG 1 cut(s) 449
MvaI CCWGG 1 cut(s) 408
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 4 cut(s) 344, 522, 568, 686
NarI GGCGCC 1 cut(s) 569
NciI CCSGG 3 cut(s) 214, 478, 620
NdeII GATC 2 cut(s) 386, 703
NlaIII CATG 1 cut(s) 37
NlaIV GGNNCC 4 cut(s) 217, 570, 645, 646
NmeAIII GCCGAG 1 cut(s) 149
PceI AGGCCT 1 cut(s) 406
PcsI WCGNNNNNNNCGW 2 cut(s) 170, 330
PfoI TCCNGGA 1 cut(s) 212
PkrI GCNGC 9 cut(s) 64, 67, 349, 352, 377, 380, 501, 515, 518
PluTI GGCGCC 1 cut(s) 572
PpuMI RGGWCCY 2 cut(s) 216, 644
Psp5II RGGWCCY 2 cut(s) 216, 644
Psp6I CCWGG 1 cut(s) 406
PspFI CCCAGC 2 cut(s) 91, 639
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 4 cut(s) 217, 570, 645, 646
PspPI GGNCC 2 cut(s) 216, 644
PspPPI RGGWCCY 2 cut(s) 216, 644
RseI CAYNNNNRTG 1 cut(s) 32
SaqAI TTAA 1 cut(s) 693
SatI GCNGC 9 cut(s) 63, 66, 348, 351, 376, 379, 500, 514, 517
Sau3AI GATC 2 cut(s) 386, 703
Sau96I GGNCC 2 cut(s) 216, 644
ScrFI CCNGG 4 cut(s) 214, 408, 478, 620
SduI GDGCHC 1 cut(s) 607
SfaNI GCATC 2 cut(s) 599, 605
SfoI GGCGCC 1 cut(s) 570
SinI GGWCC 2 cut(s) 216, 644
SmiMI CAYNNNNRTG 1 cut(s) 32
Sse9I AATT 3 cut(s) 139, 437, 449
SseBI AGGCCT 1 cut(s) 406
SsiI CCGC 8 cut(s) 63, 66, 348, 351, 378, 513, 562, 612
SspDI GGCGCC 1 cut(s) 568
StuI AGGCCT 1 cut(s) 406
StyD4I CCNGG 4 cut(s) 212, 406, 476, 618
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 3 cut(s) 74, 324, 661
TaqI TCGA 7 cut(s) 180, 366, 389, 441, 492, 607, 633
TasI AATT 3 cut(s) 139, 437, 449
TauI GCSGC 6 cut(s) 65, 68, 350, 353, 381, 516
Tru1I TTAA 1 cut(s) 693
Tru9I TTAA 1 cut(s) 693
TseI GCWGC 3 cut(s) 375, 499, 516
VpaK11BI GGWCC 2 cut(s) 216, 644
XapI RAATTY 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.