Rorug05G0462200
ERF Family

Ribosomal protein L34e superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002458
Physical Location & Seq
Forward (+)
63642179 .. 63670783
28605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0462200.1

Sequence Viewer

Length: 3552 bp
ATGGCCTCAAGCACCCAAACAGCATCCTCATCTCATCCTTCATCATCTGCTCCTCGGAGGAAGTACGATGTTTTTCTGAGTTTCCGGGGTGAAGATACTCGCAGGACTATTGTCCCCTTTTTATACCGGGAATTGCAAAACAGAGAAATTGAAACATTCATGGATGACACAGGGCTTAGCAGAGGGACGTCTATTGCTCCGACTCTGCTAACAGCAATCCAAGAATCCAGGTCAGCGATTGTTGTTCTCTCACCAAACTATGCGTATTCTAGATGGTGTTTGGACGAATTGACAATGATCATTCAGTGCATGGAATATAAGAGCACAACAGTCATCCCAATCTTTTATGATGTGGTTCCCTCTGATGTGCGACATCAGAAAGGGAGTTTTGAAAGAGCCTTCATTGAGCACCAAGAAAGGCTTGCGCAAGACGATGACTACCCAAATAAGTTGAACCAGTGGAGAGTTGCTTTAACAAAAGTTGCCGGTCTCTCTGGCTTGGAATCAAACAAATATAGTTCTGACTTAGAACTTGTCAAAGGCATTGTGGAAACTGTGTGCAGTAAACTACGACCTATTGATGCTGAGTGGGCATTGTCGTCTACCGGAGATTTTGTAGCATTCGAAGCAACAAGACGAGCCATGGACAAGGTAATGAGGGCACTTCAAGATGATGAGATCACTGTCATTGGTGTCTTTGGTATGGGAGGCGTTGGCAAGACAAGTATGGTGAAACATGTCGGTGCACAAGCCCGGAAAATTGGGCTCTTTAATCATGTGATTATGGCTGTCGTATCCCAATCACTTAATTTGAGAAATATTCAAGGCACACTGGCCGATCTGCTGGGACTAAAATTGTTGGAGGAGACAGAATTCGGTAGAGCGGTTAGATTGATGGAGAAAATAATGAAAGGAAATCGGATCCTTATAATCTTGGACGACATTTGGGAGAGAATAGATTTGTCTTCTATTGGAATACCGAGCCATGATGAGCTTCAAAAGTGCAGTTCCAAAATCTTATTCACTACAAGGAGATTGAATGTTTGCCATTCGATGGAGAGCCAAGCAAGCATTCCTCTTCCAGTCTTATCAGAAGATGATGCTTGGAACCTGTTTGTGAAAAAAGCAAGAAAGTCTTTTCATGAATCTACCAGCTTCTATGATGTAGCGAGGACGGTAGCTAAAGAATGTGCTGGTCTCCCAGTTGCATTGGTAGCGGTTGCAAGGGCACTTGGAGATAAAGACTTAGATGATTGGACGGAAGCAGCTCGACGACTAAAGGCTGCTCAACCTGTCAACTATGAAGATGAGAGAGATGTGCTCAGATGTATAAAGTTGAGCTATGATTACTTGAGACCTGATGATAACAACATTGCCAAGTCATTCTTCTTGCTTTGTTGTCTATTCCCTGAAGATTTTGATATCGAGGTAGAAGACTTGTTGGTGTATGGGATTGGAAAAGGATTATTTCAAGACGCTGACACAATCAAAGAAGCCAGAGCCAAAGCCCATTTGGTGGTAAAGTACCTGAAAGCTTCTAGCTTGCTTTTGGACAGTGAATACAGCGGATTTGTGAGGATGCACGACGTCATTCGAGATGTGGCCATATCAATTTCATCATCGGATGATGGCCCACGGTTTTTGGTTAAAGCTGGTTGTCAGTTAAAGAATTGGCCAGCAATTAATGTTGCACATGAAGACTACTCTGCAATATCGCTAATCAGGAATGAAATTCACAAGCTTCCTGAAAAGTTGGTATGTTCAAAACTCCATGTTTTATTACTACAAAGTAATGACAATATAGATGATATCCCAATCCCTTTTTTTCAAAGTTCCACTGAGTTGAGGGTCTTAGATCTTAGTGAGACTGGTATTTCGTTACTACCCTCATCATTAAGTCTCCTAACCAACCTTCAAGCTTTGTATTTAGATGATTGCAAGAGAATAGTTAATATCTCCATACTCGGAAAGCTGAAAAACCTTCAGATTCTCAGTATGAAGAACCTTGGAGGAATTCGTGATAAACAATTGCCGAAAAAAATTGGGTGCTTGACCAGTCTAAAGAAGTTGGATATCACCAATGCATGTTTTGAAACAGTTCCATCTAGATTGATATCAAATTTGAATGGATTAGAAGAATTGTACATGAAATGTGACTTTTGCAACTGGGGTAGAAGTATAGTTGAGGGTGGCTTATTTTCTTTTGCTTTTGGCAAGAATGCTAGCTTTGATGAGCTAACAGGATTATCACGTTTAAACAATTTGCAAGTTCACATATCTGATGTAGAATGCTTGCCTCGAAAGGTTGGGTTCATTCCGAATTGGGTCAACTTCAATATCTGTATCAATAGTAAAAAATATTTGGAACAGTCCTATTACGGACGCTTTTTTGAGAGGAAACAAACTGTCAAGTTGGATCCACGTCAACTTCTCTTTCGGTATGTCACTCTGAACACAAGCACGAAGAGTTTACCAGATTGGTTTATCAATGTCGTGACAGAGAGAGCAGAGATACTGGAGTACATTGGGTACGATGTCTCAGAGAACATTCTCCTACATTTCGGCCGTGGAAGATTACATTTCACCCGTGGAAGATTACATTTCGGCCGTCGAAGATTACATTTCGCCCGTCCAAGATTGCATTTCGACATTCTAGAGGAATACGACCTTGGGAGATTACATGTACTGAAGTGTCTTGCTGTAATTGAGTGCCGTGTATATGGAGGGTTGATGATGCCAGGAAGAAGGGTTCTAAATAAACCTGTGTTTGAGAGCTTGATAGATTTGTATCTGGAAGGGGTCCATGGCCTGCACTATATATGTCTTGTTAAGTTACCGCCTGGGTCTCTGTGCAATCTTAAGTTGTTAAATGTGAGAAATTGCCTGGATTTGATGGGGACACTTTTCGGTTCAGAATTGTTGCAGACGCTACCAAATCTGGAAATACTACATTGGGAGAACACGGAGGATGCGAGAGCAACCAGAATGGACCATGTGTTTGGATTTGTATTGCTGGAGCCAGAACAAATTGTCTTACCAAAACTGAGAGAGATAACATTATGTTATCTTGTGAACCTCATAAGCATATGCTATGGTCATCCTCCTAATGGAGTCTTCCGTAATCTTAACAGTCTGGCAATTCGGGGCTGCCACAAACTGAAATATCTGTTCGAGGACAATGCAGCTCAATCTCTTTTCCAATTGGAACTCCTTTGGGTGGAGGAGTGCTCTGACCTGGAAACGGTAATTGAATCAAGACAGGAAACAGAGATCAACAACATAGTTTTTCCAAAATTGAAGGAAATATATTTAAATAGTATTCCACATGTCAGAAGATTCTACAGACAAAAGACGGTTGTGTGCCCTTCATTGGAACACTTGTACGTGTACAATTGTCCCAGACTTTCAAACCTTGCTGCTTCTGACTTCCAAAGCAAGAACCACGTCCAAGCCAATAATGAAGGACATTTTGCAATCATATTTGAAAGATTGTTCCACCGCCAATTCAATCCAGAAGAGGTTCCTCAGTTCTTCTCCAAAACAGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1183

Amino Acids

134.69

Weight (kDa)

7.01

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 195 5.5e-49 TIR domain
TIR_2 PF13676 24 - 120 6.4e-15 TIR domain
NB-ARC PF00931 214 - 377 1.1e-30 NB-ARC domain
LRR_14 PF23598 572 - 725 4.2e-11 Leucine-rich repeat region
LRR_8 PF13855 589 - 646 5.9e-06 Leucine rich repeat
LRR_RPS2 PF23247 937 - 1076 1.9e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1036 - 1138 1.9e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 929
AatII GACGTC 2 cut(s) 191, 1590
Acc16I TGCGCA 1 cut(s) 426
AccB7I CCANNNNNTGG 2 cut(s) 1054, 1516
AccBSI CCGCTC 1 cut(s) 884
AccI GTMKAC 1 cut(s) 602
AciI CCGC 5 cut(s) 884, 1217, 1566, 2837, 3499
AclWI GGATC 4 cut(s) 916, 929, 2411, 2424
AcoI YGGCCR 5 cut(s) 834, 1602, 1673, 2563, 2605
AcsI RAATTY 4 cut(s) 872, 1731, 2013, 2119
AcuI CTGAAG 3 cut(s) 1431, 1967, 2708
AcyI GRCGYC 2 cut(s) 188, 1587
AfaI GTAC 8 cut(s) 65, 1526, 2144, 2522, 2531, 2685, 3383, 3389
AfiI CCNNNNNNNGG 6 cut(s) 1054, 1516, 3107, 3217, 3241, 3370
AflII CTTAAG 1 cut(s) 2858
AflIII ACRYGT 4 cut(s) 736, 2680, 3325, 3384
AjiI CACGTC 2 cut(s) 2423, 3445
AjnI CCWGG 5 cut(s) 227, 2737, 2839, 2883, 3234
AjuI GAANNNNNNNTTGG 8 cut(s) 1004, 1036, 1056, 1088, 1370, 1402, 2652, 2684
AloI GAACNNNNNNTCC 2 cut(s) 2733, 2765
Alw21I GWGCWC 5 cut(s) 326, 411, 748, 1323, 3230
Alw26I GTCTC 8 cut(s) 494, 860, 1202, 1348, 1859, 1904, 2542, 2850
Alw44I GTGCAC 1 cut(s) 744
AlwI GGATC 4 cut(s) 916, 929, 2411, 2424
AoxI GGCC 8 cut(s) 3, 834, 1602, 1630, 1673, 2563, 2605, 2806
ApaLI GTGCAC 1 cut(s) 744
ApeKI GCWGC 5 cut(s) 1265, 1283, 3147, 3182, 3416
ApoI RAATTY 4 cut(s) 872, 1731, 2013, 2119
ArsI GACNNNNNNTTYG 4 cut(s) 1858, 1890, 2981, 3013
AseI ATTAAT 1 cut(s) 1683
Asp700I GAANNNNTTC 2 cut(s) 2097, 3519
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 3 cut(s) 1631, 2800, 2989
AsuC2I CCSGG 3 cut(s) 86, 128, 754
AsuHPI GGTGA 5 cut(s) 101, 243, 742, 2068, 2575
AsuII TTCGAA 1 cut(s) 624
AsuNHI GCTAGC 1 cut(s) 2222
AvaII GGWCC 2 cut(s) 2800, 2989
BaeGI GKGCMC 4 cut(s) 664, 748, 1231, 3365
BaeI ACNNNNGTAYC 2 cut(s) 2504, 2537
BalI TGGCCA 2 cut(s) 1604, 1675
BamHI GGATCC 2 cut(s) 921, 2416
BanII GRGCYC 1 cut(s) 768
BbsI GAAGAC 4 cut(s) 957, 1440, 1704, 3106
Bbv12I GWGCWC 5 cut(s) 326, 411, 748, 1323, 3230
BbvI GCAGC 5 cut(s) 1270, 1277, 3134, 3194, 3403
BccI CCATC 6 cut(s) 267, 889, 1048, 1622, 2110, 2887
BceAI ACGGC 3 cut(s) 2550, 2592, 2697
BcgI CGANNNNNNTGC 2 cut(s) 3161, 3195
BciT130I CCWGG 5 cut(s) 229, 2739, 2841, 2885, 3236
BciVI GTATCC 1 cut(s) 805
BclI TGATCA 1 cut(s) 297
BcnI CCSGG 3 cut(s) 86, 128, 754
BcoDI GTCTC 8 cut(s) 494, 860, 1202, 1348, 1859, 1904, 2542, 2850
BfaI CTAG 5 cut(s) 270, 1539, 2106, 2223, 2654
BfmI CTRYAG 1 cut(s) 3340
BfrI CTTAAG 1 cut(s) 2858
BfuI GTATCC 1 cut(s) 805
BglII AGATCT 1 cut(s) 1855
BisI GCNGC 5 cut(s) 1266, 1284, 3148, 3183, 3417
BlpI GCTNAGC 1 cut(s) 176
BlsI GCNGC 5 cut(s) 1267, 1285, 3149, 3184, 3418
Bme1390I CCNGG 8 cut(s) 86, 128, 229, 754, 2739, 2841, 2885, 3236
Bme18I GGWCC 2 cut(s) 2800, 2989
BmgBI CACGTC 2 cut(s) 2423, 3445
BmgT120I GGNCC 3 cut(s) 1631, 2800, 2989
BmiI GGNNCC 7 cut(s) 357, 923, 1109, 2418, 2801, 3018, 3522
BmrFI CCNGG 8 cut(s) 86, 128, 229, 754, 2739, 2841, 2885, 3236
BmrI ACTGGG 2 cut(s) 1196, 2176
BmsI GCATC 6 cut(s) 32, 571, 1090, 1569, 2724, 2959
BmtI GCTAGC 1 cut(s) 2226
BmuI ACTGGG 2 cut(s) 1196, 2176
BoxI GACNNNNGTC 1 cut(s) 110
BpiI GAAGAC 4 cut(s) 957, 1440, 1704, 3106
BplI GAGNNNNNCTC 4 cut(s) 1305, 1337, 3212, 3244
BpmI CTGGAG 2 cut(s) 2537, 3035
Bpu1102I GCTNAGC 1 cut(s) 176
Bpu14I TTCGAA 1 cut(s) 624
BpuEI CTTGAG 1 cut(s) 1372
BpuMI CCSGG 3 cut(s) 86, 128, 754
BsaAI YACGTR 1 cut(s) 3385
BsaBI GATNNNNATC 2 cut(s) 2113, 2787
BsaHI GRCGYC 2 cut(s) 188, 1587
BsaI GGTCTC 4 cut(s) 494, 1202, 1348, 2850
BsaWI WCCGGW 1 cut(s) 605
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bsc4I CCNNNNNNNGG 6 cut(s) 1054, 1516, 3107, 3217, 3241, 3370
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 8 cut(s) 457, 837, 1082, 1202, 1873, 2055, 2171, 2520
Bse3DI GCAATG 1 cut(s) 1371
Bse8I GATNNNNATC 2 cut(s) 2113, 2787
BseBI CCWGG 5 cut(s) 229, 2739, 2841, 2885, 3236
BseGI GGATG 8 cut(s) 23, 34, 169, 333, 1584, 1630, 2974, 3097
BseJI GATNNNNATC 2 cut(s) 2113, 2787
BseLI CCNNNNNNNGG 6 cut(s) 1054, 1516, 3107, 3217, 3241, 3370
BseMI GCAATG 1 cut(s) 1371
BseMII CTCAG 8 cut(s) 68, 576, 1336, 1830, 2005, 2553, 3035, 3539
BseNI ACTGG 8 cut(s) 457, 837, 1082, 1202, 1873, 2055, 2171, 2520
BseRI GAGGAG 3 cut(s) 42, 878, 3236
BseSI GKGCMC 4 cut(s) 664, 748, 1231, 3365
BseX3I CGGCCG 2 cut(s) 2563, 2605
BseXI GCAGC 5 cut(s) 1270, 1277, 3134, 3194, 3403
BseYI CCCAGC 1 cut(s) 844
BsgI GTGCAG 3 cut(s) 580, 1024, 2795
Bsh1285I CGRYCG 2 cut(s) 2566, 2608
BshFI GGCC 8 cut(s) 5, 836, 1604, 1632, 1675, 2565, 2607, 2808
BsiEI CGRYCG 2 cut(s) 2566, 2608
BsiHKAI GWGCWC 5 cut(s) 326, 411, 748, 1323, 3230
BsiSI CCGG 5 cut(s) 85, 127, 486, 606, 754
BslFI GGGAC 5 cut(s) 98, 199, 861, 2911, 3381
BslI CCNNNNNNNGG 6 cut(s) 1054, 1516, 3107, 3217, 3241, 3370
BsmAI GTCTC 8 cut(s) 494, 860, 1202, 1348, 1859, 1904, 2542, 2850
BsmFI GGGAC 5 cut(s) 98, 199, 861, 2911, 3381
BsmI GAATGC 4 cut(s) 620, 1071, 2224, 2294
BsnI GGCC 8 cut(s) 5, 836, 1604, 1632, 1675, 2565, 2607, 2808
Bso31I GGTCTC 4 cut(s) 494, 1202, 1348, 2850
Bsp119I TTCGAA 1 cut(s) 624
Bsp1286I GDGCHC 9 cut(s) 326, 411, 664, 748, 768, 1231, 1323, 3230, 3365
Bsp1407I TGTACA 2 cut(s) 2142, 3387
Bsp143I GATC 7 cut(s) 297, 678, 838, 921, 1855, 2416, 3270
Bsp1720I GCTNAGC 1 cut(s) 176
Bsp19I CCATGG 2 cut(s) 642, 2803
BspACI CCGC 5 cut(s) 884, 1217, 1566, 2837, 3499
BspANI GGCC 8 cut(s) 5, 836, 1604, 1632, 1675, 2565, 2607, 2808
BspCNI CTCAG 8 cut(s) 69, 577, 1335, 1831, 2004, 2552, 3036, 3538
BspHI TCATGA 1 cut(s) 1141
BspLI GGNNCC 7 cut(s) 357, 923, 1109, 2418, 2801, 3018, 3522
BspOI GCTAGC 1 cut(s) 2226
BspPI GGATC 4 cut(s) 916, 929, 2411, 2424
BspT104I TTCGAA 1 cut(s) 624
BspTI CTTAAG 1 cut(s) 2858
BspTNI GGTCTC 4 cut(s) 494, 1202, 1348, 2850
BsrBI CCGCTC 1 cut(s) 884
BsrDI GCAATG 1 cut(s) 1371
BsrFI RCCGGY 1 cut(s) 485
BsrGI TGTACA 2 cut(s) 2142, 3387
BsrI ACTGG 8 cut(s) 457, 837, 1082, 1202, 1873, 2055, 2171, 2520
BssAI RCCGGY 1 cut(s) 485
BssMI GATC 7 cut(s) 297, 678, 838, 921, 1855, 2416, 3270
BssNI GRCGYC 2 cut(s) 188, 1587
BssT1I CCWWGG 4 cut(s) 642, 2005, 2668, 2803
Bst2UI CCWGG 5 cut(s) 229, 2739, 2841, 2885, 3236
Bst6I CTCTTC 3 cut(s) 1083, 2459, 3510
BstACI GRCGYC 2 cut(s) 188, 1587
BstAFI CTTAAG 1 cut(s) 2858
BstAUI TGTACA 2 cut(s) 2142, 3387
BstBAI YACGTR 1 cut(s) 3385
BstBI TTCGAA 1 cut(s) 624
BstC8I GCNNGC 7 cut(s) 423, 1069, 1544, 1677, 2224, 2294, 2810
BstDSI CCRYGG 5 cut(s) 642, 1634, 2566, 2587, 2803
BstF5I GGATG 8 cut(s) 23, 34, 169, 333, 1584, 1630, 2974, 3097
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 7 cut(s) 300, 681, 841, 924, 1858, 2419, 3273
BstMAI GTCTC 8 cut(s) 494, 860, 1202, 1348, 1859, 1904, 2542, 2850
BstMBI GATC 7 cut(s) 297, 678, 838, 921, 1855, 2416, 3270
BstMCI CGRYCG 2 cut(s) 2566, 2608
BstMWI GCNNNNNNNGC 4 cut(s) 590, 626, 1068, 1214
BstNI CCWGG 5 cut(s) 229, 2739, 2841, 2885, 3236
BstNSI RCATGY 4 cut(s) 740, 2088, 2684, 3329
BstPAI GACNNNNGTC 1 cut(s) 110
BstSCI CCNGG 8 cut(s) 84, 126, 227, 752, 2737, 2839, 2883, 3234
BstSFI CTRYAG 1 cut(s) 3340
BstSLI GKGCMC 4 cut(s) 664, 748, 1231, 3365
BstV1I GCAGC 5 cut(s) 1270, 1277, 3134, 3194, 3403
BstV2I GAAGAC 4 cut(s) 957, 1440, 1704, 3106
BstX2I RGATCY 3 cut(s) 921, 1855, 2416
BstXI CCANNNNNNTGG 1 cut(s) 2999
BstYI RGATCY 3 cut(s) 921, 1855, 2416
BstZI CGGCCG 2 cut(s) 2563, 2605
BsuI GTATCC 1 cut(s) 805
BsuRI GGCC 8 cut(s) 5, 836, 1604, 1632, 1675, 2565, 2607, 2808
BtgI CCRYGG 5 cut(s) 642, 1634, 2566, 2587, 2803
BtrI CACGTC 2 cut(s) 2423, 3445
BtsCI GGATG 8 cut(s) 23, 34, 169, 333, 1584, 1630, 2974, 3097
BtsIMutI CAGTG 6 cut(s) 311, 464, 681, 830, 1561, 1836
Cac8I GCNNGC 7 cut(s) 423, 1069, 1544, 1677, 2224, 2294, 2810
CciI TCATGA 1 cut(s) 1141
CfoI GCGC 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 485
Cfr13I GGNCC 3 cut(s) 1631, 2800, 2989
CseI GACGC 3 cut(s) 1484, 2391, 2935
Csp6I GTAC 8 cut(s) 64, 1525, 2143, 2521, 2530, 2684, 3382, 3388
CspCI CAANNNNNGTGG 2 cut(s) 1623, 1658
CviQI GTAC 8 cut(s) 64, 1525, 2143, 2521, 2530, 2684, 3382, 3388
DpnI GATC 7 cut(s) 299, 680, 840, 923, 1857, 2418, 3272
DpnII GATC 7 cut(s) 297, 678, 838, 921, 1855, 2416, 3270
DraI TTTAAA 2 cut(s) 2256, 3312
EaeI YGGCCR 5 cut(s) 834, 1602, 1673, 2563, 2605
EagI CGGCCG 2 cut(s) 2563, 2605
Eam1104I CTCTTC 3 cut(s) 1083, 2459, 3510
EarI CTCTTC 3 cut(s) 1083, 2459, 3510
EclXI CGGCCG 2 cut(s) 2563, 2605
Eco130I CCWWGG 4 cut(s) 642, 2005, 2668, 2803
Eco24I GRGCYC 1 cut(s) 768
Eco31I GGTCTC 4 cut(s) 494, 1202, 1348, 2850
Eco32I GATATC 4 cut(s) 1423, 1810, 2074, 2115
Eco47I GGWCC 2 cut(s) 2800, 2989
Eco52I CGGCCG 2 cut(s) 2563, 2605
Eco57I CTGAAG 3 cut(s) 1431, 1967, 2708
EcoRI GAATTC 2 cut(s) 872, 2013
EcoRII CCWGG 5 cut(s) 227, 2737, 2839, 2883, 3234
EcoRV GATATC 4 cut(s) 1423, 1810, 2074, 2115
EcoT14I CCWWGG 4 cut(s) 642, 2005, 2668, 2803
EcoT22I ATGCAT 1 cut(s) 2086
EcoT38I GRGCYC 1 cut(s) 768
ErhI CCWWGG 4 cut(s) 642, 2005, 2668, 2803
FalI AAGNNNNNCTT 6 cut(s) 405, 437, 1120, 1152, 1370, 1402
FaqI GGGAC 5 cut(s) 98, 199, 861, 2911, 3381
FauNDI CATATG 1 cut(s) 3086
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 1 cut(s) 602
Fnu4HI GCNGC 5 cut(s) 1266, 1284, 3148, 3183, 3417
FokI GGATG 8 cut(s) 10, 21, 176, 320, 1591, 1637, 2981, 3084
FriOI GRGCYC 1 cut(s) 768
Fsp4HI GCNGC 5 cut(s) 1266, 1284, 3148, 3183, 3417
FspBI CTAG 5 cut(s) 270, 1539, 2106, 2223, 2654
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 1 cut(s) 426
GluI GCNGC 5 cut(s) 1266, 1284, 3148, 3183, 3417
GsaI CCCAGC 1 cut(s) 848
GsuI CTGGAG 2 cut(s) 2537, 3035
HaeIII GGCC 8 cut(s) 5, 836, 1604, 1632, 1675, 2565, 2607, 2808
HapII CCGG 5 cut(s) 85, 127, 486, 606, 754
HgaI GACGC 3 cut(s) 1484, 2391, 2935
HhaI GCGC 1 cut(s) 427
Hin1I GRCGYC 2 cut(s) 188, 1587
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HincII GTYRAC 3 cut(s) 1297, 2329, 2426
HindII GTYRAC 3 cut(s) 1297, 2329, 2426
HindIII AAGCTT 3 cut(s) 1533, 1739, 1917
HinfI GANTC 8 cut(s) 202, 224, 503, 1145, 1987, 3111, 3251, 3336
HpaII CCGG 5 cut(s) 85, 127, 486, 606, 754
HphI GGTGA 5 cut(s) 101, 243, 742, 2068, 2575
Hpy99I CGWCG 3 cut(s) 1275, 1589, 2613
HpyAV CCTTC 9 cut(s) 48, 409, 1922, 1991, 2739, 2789, 3292, 3375, 3455
HpyCH4IV ACGT 6 cut(s) 188, 1587, 2251, 2422, 3384, 3444
HpyF10VI GCNNNNNNNGC 4 cut(s) 590, 626, 1068, 1214
HpySE526I ACGT 6 cut(s) 188, 1587, 2251, 2422, 3384, 3444
Hsp92I GRCGYC 2 cut(s) 188, 1587
HspAI GCGC 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 7 cut(s) 297, 678, 838, 921, 1855, 2416, 3270
LmnI GCTCC 3 cut(s) 55, 202, 3016
Lsp1109I GCAGC 5 cut(s) 1270, 1277, 3134, 3194, 3403
LweI GCATC 6 cut(s) 32, 571, 1090, 1569, 2724, 2959
MaeI CTAG 5 cut(s) 270, 1539, 2106, 2223, 2654
MaeII ACGT 6 cut(s) 188, 1587, 2251, 2422, 3384, 3444
MaeIII GTNAC 5 cut(s) 1878, 2153, 2443, 2494, 2832
MalI GATC 7 cut(s) 299, 680, 840, 923, 1857, 2418, 3272
MbiI CCGCTC 1 cut(s) 884
MboI GATC 7 cut(s) 297, 678, 838, 921, 1855, 2416, 3270
MfeI CAATTG 3 cut(s) 2027, 3200, 3391
MflI RGATCY 3 cut(s) 921, 1855, 2416
MhlI GDGCHC 9 cut(s) 326, 411, 664, 748, 768, 1231, 1323, 3230, 3365
MlsI TGGCCA 2 cut(s) 1604, 1675
MluNI TGGCCA 2 cut(s) 1604, 1675
MlyI GAGTC 2 cut(s) 196, 3120
MmeI TCCRAC 4 cut(s) 224, 840, 2049, 2394
Mox20I TGGCCA 2 cut(s) 1604, 1675
Mph1103I ATGCAT 1 cut(s) 2086
MroXI GAANNNNTTC 2 cut(s) 2097, 3519
MscI TGGCCA 2 cut(s) 1604, 1675
MslI CAYNNNNRTG 1 cut(s) 741
Msp20I TGGCCA 2 cut(s) 1604, 1675
MspA1I CMGCKG 1 cut(s) 1566
MspCI CTTAAG 1 cut(s) 2858
MspI CCGG 5 cut(s) 85, 127, 486, 606, 754
MspR9I CCNGG 8 cut(s) 86, 128, 229, 754, 2739, 2841, 2885, 3236
MssI GTTTAAAC 1 cut(s) 2256
MunI CAATTG 3 cut(s) 2027, 3200, 3391
Mva1269I GAATGC 4 cut(s) 620, 1071, 2224, 2294
MvaI CCWGG 5 cut(s) 229, 2739, 2841, 2885, 3236
MwoI GCNNNNNNNGC 4 cut(s) 590, 626, 1068, 1214
NciI CCSGG 3 cut(s) 86, 128, 754
NcoI CCATGG 2 cut(s) 642, 2803
NdeI CATATG 1 cut(s) 3086
NdeII GATC 7 cut(s) 297, 678, 838, 921, 1855, 2416, 3270
NheI GCTAGC 1 cut(s) 2222
NlaIV GGNNCC 7 cut(s) 357, 923, 1109, 2418, 2801, 3018, 3522
NmuCI GTSAC 3 cut(s) 2153, 2443, 2494
NsbI TGCGCA 1 cut(s) 426
NsiI ATGCAT 1 cut(s) 2086
NspI RCATGY 4 cut(s) 740, 2088, 2684, 3329
NspV TTCGAA 1 cut(s) 624
PagI TCATGA 1 cut(s) 1141
PciI ACATGT 3 cut(s) 736, 2680, 3325
PcsI WCGNNNNNNNCGW 1 cut(s) 2969
PctI GAATGC 4 cut(s) 620, 1071, 2224, 2294
PdmI GAANNNNTTC 2 cut(s) 2097, 3519
PfeI GAWTC 6 cut(s) 224, 503, 1145, 1987, 3251, 3336
PflMI CCANNNNNTGG 2 cut(s) 1054, 1516
PkrI GCNGC 5 cut(s) 1267, 1285, 3149, 3184, 3418
PleI GAGTC 2 cut(s) 196, 3119
PmeI GTTTAAAC 1 cut(s) 2256
PpsI GAGTC 2 cut(s) 196, 3119
Ppu21I YACGTR 1 cut(s) 3385
PscI ACATGT 3 cut(s) 736, 2680, 3325
PshAI GACNNNNGTC 1 cut(s) 110
PshBI ATTAAT 1 cut(s) 1683
PsiI TTATAA 1 cut(s) 929
Psp6I CCWGG 5 cut(s) 227, 2737, 2839, 2883, 3234
PspFI CCCAGC 1 cut(s) 844
PspGI CCWGG 5 cut(s) 227, 2737, 2839, 2883, 3234
PspN4I GGNNCC 7 cut(s) 357, 923, 1109, 2418, 2801, 3018, 3522
PspPI GGNCC 3 cut(s) 1631, 2800, 2989
PsuI RGATCY 3 cut(s) 921, 1855, 2416
RsaI GTAC 8 cut(s) 65, 1526, 2144, 2522, 2531, 2685, 3383, 3389
RsaNI GTAC 8 cut(s) 64, 1525, 2143, 2521, 2530, 2684, 3382, 3388
RseI CAYNNNNRTG 1 cut(s) 741
SatI GCNGC 5 cut(s) 1266, 1284, 3148, 3183, 3417
Sau3AI GATC 7 cut(s) 297, 678, 838, 921, 1855, 2416, 3270
Sau96I GGNCC 3 cut(s) 1631, 2800, 2989
SchI GAGTC 2 cut(s) 196, 3120
ScrFI CCNGG 8 cut(s) 86, 128, 229, 754, 2739, 2841, 2885, 3236
SduI GDGCHC 9 cut(s) 326, 411, 664, 748, 768, 1231, 1323, 3230, 3365
SfaNI GCATC 6 cut(s) 32, 571, 1090, 1569, 2724, 2959
SfcI CTRYAG 1 cut(s) 3340
SfuI TTCGAA 1 cut(s) 624
SinI GGWCC 2 cut(s) 2800, 2989
SmiI ATTTAAAT 1 cut(s) 3312
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 3 cut(s) 7, 1351, 2858
SmoI CTYRAG 3 cut(s) 7, 1351, 2858
SsiI CCGC 5 cut(s) 884, 1217, 1566, 2837, 3499
SspI AATATT 2 cut(s) 820, 2360
SspMI CTAG 5 cut(s) 270, 1539, 2106, 2223, 2654
StyD4I CCNGG 8 cut(s) 84, 126, 227, 752, 2737, 2839, 2883, 3234
StyI CCWWGG 4 cut(s) 642, 2005, 2668, 2803
SwaI ATTTAAAT 1 cut(s) 3312
TaiI ACGT 6 cut(s) 191, 1590, 2254, 2425, 3387, 3447
TaqI TCGA 9 cut(s) 624, 1052, 1270, 1425, 1594, 2299, 2611, 2646, 3171
TatI WGTACW 4 cut(s) 2142, 2520, 2683, 3387
TfiI GAWTC 6 cut(s) 224, 503, 1145, 1987, 3251, 3336
TscAI CASTG 6 cut(s) 311, 464, 688, 837, 1561, 1843
TseFI GTSAC 3 cut(s) 2153, 2443, 2494
TseI GCWGC 5 cut(s) 1265, 1283, 3147, 3182, 3416
Tsp45I GTSAC 3 cut(s) 2153, 2443, 2494
TspGWI ACGGA 4 cut(s) 1274, 2394, 2978, 3107
TspRI CASTG 6 cut(s) 311, 464, 688, 837, 1561, 1843
Van91I CCANNNNNTGG 2 cut(s) 1054, 1516
Vha464I CTTAAG 1 cut(s) 2858
VneI GTGCAC 1 cut(s) 744
VpaK11BI GGWCC 2 cut(s) 2800, 2989
VspI ATTAAT 1 cut(s) 1683
XapI RAATTY 4 cut(s) 872, 1731, 2013, 2119
XbaI TCTAGA 3 cut(s) 269, 2105, 2653
XceI RCATGY 4 cut(s) 740, 2088, 2684, 3329
XcmI CCANNNNNNNNNTGG 1 cut(s) 1510
XmiI GTMKAC 1 cut(s) 602
XmnI GAANNNNTTC 2 cut(s) 2097, 3519
XspI CTAG 5 cut(s) 270, 1539, 2106, 2223, 2654
ZraI GACGTC 2 cut(s) 189, 1588
Zsp2I ATGCAT 1 cut(s) 2086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.