Rorug04G0153700
ERF Family

Ribosomal protein L34e superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002458
Physical Location & Seq
Forward (+)
26957150 .. 26957670
521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0153700.1

Sequence Viewer

Length: 381 bp
ATGGGTTTTCTCAGTAAAGTCTCCGAGCTCCGTGCTAGGGCTCGGAGTCATAAAAAGCTCAAGAAGAACAAGCAGCTACAGACTGTGGAGATAAAAGTGAAGATGGACTGCGAAGGTTGCGAGAGAAGAGTGAAGAAATCAGTGGAAGGCATGAAAGGAGTCACAAGTGTTGAAGTGGATCCCAAGCAAAGCAAGCTCACTGTTATTGGCTATGTGGACCCCAACAAGGTGTTGCATCGTGTCAGGCATCGAACGGGGAAGAAGGCGGATCTGTGGCCGTATGTACCATACGACGTCGTCAAGCATCCATGGGCTCCCGGGGCTTATGATAGGAAGGCACCACCTGGGTACGTGAGGAACTTGCTGAATGACCCTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

14.42

Weight (kDa)

9.97

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 32 - 87 4e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 297
AccB1I GGYRCC 1 cut(s) 337
AciI CCGC 1 cut(s) 266
AclWI GGATC 3 cut(s) 173, 186, 276
AcoI YGGCCR 1 cut(s) 275
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 2 cut(s) 285, 350
AfiI CCNNNNNNNGG 2 cut(s) 37, 226
AgsI TTSAA 1 cut(s) 173
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 4 cut(s) 28, 58, 76, 196
AluI AGCT 4 cut(s) 28, 58, 76, 196
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 25
AlwI GGATC 3 cut(s) 173, 186, 276
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 2 cut(s) 318, 319
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 217
BamHI GGATCC 1 cut(s) 178
BanI GGYRCC 1 cut(s) 337
BanII GRGCYC 3 cut(s) 30, 43, 316
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 85
BccI CCATC 1 cut(s) 97
BceAI ACGGC 1 cut(s) 262
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 1 cut(s) 345
BcnI CCSGG 2 cut(s) 318, 319
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 2 cut(s) 36, 379
BfmI CTRYAG 1 cut(s) 77
BisI GCNGC 1 cut(s) 74
BlsI GCNGC 1 cut(s) 75
Bme1390I CCNGG 3 cut(s) 318, 319, 345
Bme18I GGWCC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 4 cut(s) 180, 219, 315, 339
BmrFI CCNGG 3 cut(s) 318, 319, 345
BmsI GCATC 3 cut(s) 244, 256, 313
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 2 cut(s) 318, 319
BsaAI YACGTR 1 cut(s) 352
BsaHI GRCGYC 1 cut(s) 294
BsaJI CCNNGG 4 cut(s) 308, 317, 318, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 226
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 4 cut(s) 308, 317, 318, 344
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 2 cut(s) 37, 226
BseMII CTCAG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 85
BshFI GGCC 1 cut(s) 277
BshNI GGYRCC 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 37, 226
BsmAI GTCTC 1 cut(s) 25
BsnI GGCC 1 cut(s) 277
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 3 cut(s) 30, 43, 316
Bsp143I GATC 2 cut(s) 178, 268
Bsp19I CCATGG 1 cut(s) 308
BspACI CCGC 1 cut(s) 266
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 1 cut(s) 24
BspLI GGNNCC 4 cut(s) 180, 219, 315, 339
BspPI GGATC 3 cut(s) 173, 186, 276
BspT107I GGYRCC 1 cut(s) 337
BssECI CCNNGG 4 cut(s) 308, 317, 318, 344
BssMI GATC 2 cut(s) 178, 268
BssNI GRCGYC 1 cut(s) 294
BssT1I CCWWGG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 85, 202
Bst6I CTCTTC 1 cut(s) 121
BstACI GRCGYC 1 cut(s) 294
BstBAI YACGTR 1 cut(s) 352
BstC8I GCNNGC 1 cut(s) 194
BstDEI CTNAG 1 cut(s) 11
BstDSI CCRYGG 1 cut(s) 308
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 2 cut(s) 181, 271
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 2 cut(s) 178, 268
BstMWI GCNNNNNNNGC 3 cut(s) 117, 193, 320
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 3 cut(s) 316, 317, 343
BstSFI CTRYAG 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 85
BstX2I RGATCY 2 cut(s) 178, 268
BstYI RGATCY 2 cut(s) 178, 268
BsuRI GGCC 1 cut(s) 277
BtgI CCRYGG 1 cut(s) 308
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 2 cut(s) 147, 198
Cac8I GCNNGC 1 cut(s) 194
Cfr13I GGNCC 1 cut(s) 217
Cfr9I CCCGGG 1 cut(s) 317
Csp6I GTAC 2 cut(s) 284, 349
CviAII CATG 2 cut(s) 151, 309
CviJI RGCY 9 cut(s) 28, 41, 58, 76, 196, 210, 277, 314, 323
CviKI_1 RGCY 9 cut(s) 28, 41, 58, 76, 196, 210, 277, 314, 323
CviQI GTAC 2 cut(s) 284, 349
DdeI CTNAG 1 cut(s) 11
DpnI GATC 2 cut(s) 180, 270
DpnII GATC 2 cut(s) 178, 268
EaeI YGGCCR 1 cut(s) 275
Eam1104I CTCTTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 121
EciI GGCGGA 1 cut(s) 281
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 308
Eco24I GRGCYC 3 cut(s) 30, 43, 316
Eco47I GGWCC 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 28
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 343
EcoT14I CCWWGG 1 cut(s) 308
EcoT38I GRGCYC 3 cut(s) 30, 43, 316
ErhI CCWWGG 1 cut(s) 308
FaeI CATG 2 cut(s) 154, 312
FaiI YATR 7 cut(s) 51, 152, 213, 282, 289, 310, 327
FatI CATG 2 cut(s) 150, 308
Fnu4HI GCNGC 1 cut(s) 74
FokI GGATG 1 cut(s) 291
FriOI GRGCYC 3 cut(s) 30, 43, 316
Fsp4HI GCNGC 1 cut(s) 74
FspBI CTAG 2 cut(s) 36, 379
GluI GCNGC 1 cut(s) 74
HaeIII GGCC 1 cut(s) 277
HapII CCGG 1 cut(s) 318
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 2 cut(s) 154, 312
HinfI GANTC 2 cut(s) 46, 159
HpaII CCGG 1 cut(s) 318
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 2 cut(s) 25, 45
Hpy188III TCNNGA 1 cut(s) 61
Hpy8I GTNNAC 1 cut(s) 217
Hpy99I CGWCG 2 cut(s) 296, 299
HpyAV CCTTC 4 cut(s) 107, 140, 256, 328
HpyCH4III ACNGT 2 cut(s) 85, 202
HpyCH4IV ACGT 2 cut(s) 294, 351
HpyCH4V TGCA 1 cut(s) 235
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 193, 320
HpyF3I CTNAG 1 cut(s) 11
HpySE526I ACGT 2 cut(s) 294, 351
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 2 cut(s) 154, 312
Kzo9I GATC 2 cut(s) 178, 268
LmnI GCTCC 2 cut(s) 33, 319
LpnPI CCDG 4 cut(s) 229, 330, 331, 357
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 3 cut(s) 244, 256, 313
MaeI CTAG 2 cut(s) 36, 379
MaeII ACGT 2 cut(s) 294, 351
MaeIII GTNAC 1 cut(s) 160
MalI GATC 2 cut(s) 180, 270
MboI GATC 2 cut(s) 178, 268
MboII GAAGA 5 cut(s) 76, 112, 138, 145, 271
MflI RGATCY 2 cut(s) 178, 268
MhlI GDGCHC 3 cut(s) 30, 43, 316
MlyI GAGTC 2 cut(s) 55, 168
MnlI CCTC 1 cut(s) 348
MspI CCGG 1 cut(s) 318
MspR9I CCNGG 3 cut(s) 318, 319, 345
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 3 cut(s) 117, 193, 320
NciI CCSGG 2 cut(s) 318, 319
NcoI CCATGG 1 cut(s) 308
NdeII GATC 2 cut(s) 178, 268
NlaIII CATG 2 cut(s) 154, 312
NlaIV GGNNCC 4 cut(s) 180, 219, 315, 339
NmuCI GTSAC 1 cut(s) 160
PflFI GACNNNGTC 1 cut(s) 296
PkrI GCNGC 1 cut(s) 75
PleI GAGTC 2 cut(s) 54, 167
PpsI GAGTC 2 cut(s) 54, 167
Ppu21I YACGTR 1 cut(s) 352
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 4 cut(s) 180, 219, 315, 339
PspPI GGNCC 1 cut(s) 217
PsuI RGATCY 2 cut(s) 178, 268
PsyI GACNNNGTC 1 cut(s) 296
RsaI GTAC 2 cut(s) 285, 350
RsaNI GTAC 2 cut(s) 284, 349
SacI GAGCTC 1 cut(s) 30
SatI GCNGC 1 cut(s) 74
Sau3AI GATC 2 cut(s) 178, 268
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 2 cut(s) 55, 168
ScrFI CCNGG 3 cut(s) 318, 319, 345
SduI GDGCHC 3 cut(s) 30, 43, 316
SetI ASST 9 cut(s) 30, 60, 78, 118, 198, 231, 297, 346, 354
SfaNI GCATC 3 cut(s) 244, 256, 313
SfcI CTRYAG 1 cut(s) 77
SinI GGWCC 1 cut(s) 217
SmaI CCCGGG 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
SsiI CCGC 1 cut(s) 266
SspMI CTAG 2 cut(s) 36, 379
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 316, 317, 343
StyI CCWWGG 1 cut(s) 308
TaaI ACNGT 2 cut(s) 85, 202
TaiI ACGT 2 cut(s) 297, 354
TaqI TCGA 1 cut(s) 250
TscAI CASTG 2 cut(s) 147, 205
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 1 cut(s) 73
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 1 cut(s) 167
TspGWI ACGGA 1 cut(s) 20
TspMI CCCGGG 1 cut(s) 317
TspRI CASTG 2 cut(s) 147, 205
Tth111I GACNNNGTC 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 217
XmaI CCCGGG 1 cut(s) 317
XspI CTAG 2 cut(s) 36, 379
ZraI GACGTC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.