pycom13g16870

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005550
Physical Location & Seq
Reverse (-)
12578657 .. 12580859
2203 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
ATGGCCACCTCAGCTTCCTTTTATTTTCTCGCCGTCCTCTTTGCATTCTTCTCAGTGAAAACAATGGCGACAATTGTCAAGGCCGACAAAGTGTGTTATTTGGCGACGATCGGCGGGGGTTGTCCTGATGAGTACCAGTGTGCCCTGACATGCAGTACTTGTTACCGCGGCGTTGGCAAGGTCGCATCATATTGCAGAGCTGGTGGTGGAGGGTTTCCATATGATAGTTGTGTTTGTGCGATGTCGGACGGTGCTCCATGCAGTCCTTCTGGTCCGCCCAAGTGTCCAAACTGGCCTCGACCTCATTCTTCAACCAATCTTATTGAAATCATGATAACACAAAAAATAAAAAGTAAATATAACTCCAATTTCGTAGCCCAAAGGGCACAGAACAACAGTGCGTCCCAGCTCCAAAGCAATCAAAATCCCAGAGATCGAACCTCCAAAACCCACCAGATCAAACAAGCAATGGAGTCCATCTTCGGCTTGGTAGGCGATGGCTTTGCGGTCGTGGCGGCGGACACTTCAGCCGTGCATAGCATACTGGTGCACAAGTCCAACGAGGACAAGATCATGGTGCTCGACTCCCACAAGCTCGTCGCCGCCAGCGGCGAGCCCGGCGACAGGGTTCAGTTTACGGAGTACATACAGAAGAACGTGGCTTTGTACCAGTTCCGGAATGGGATTCCGTTGACGACCGCTGCTGCTGCCAATTTCACCCGCGGCGAACTTGCCACCGCCTTGCGAAAGGTATGGGCTTTTTCTTGA

Protein Analysis

256

Amino Acids

27.48

Weight (kDa)

8.98

Isoelectric Point (pI)

26.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 159 - 245 7e-16 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 168, 723
AccIII TCCGGA 1 cut(s) 675
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 510
AfaI GTAC 4 cut(s) 134, 157, 644, 668
AfiI CCNNNNNNNGG 1 cut(s) 624
AgsI TTSAA 2 cut(s) 312, 326
AluBI AGCT 4 cut(s) 14, 200, 409, 595
AluI AGCT 4 cut(s) 14, 200, 409, 595
Alw21I GWGCWC 3 cut(s) 256, 552, 582
Alw44I GTGCAC 1 cut(s) 548
Aor13HI TCCGGA 1 cut(s) 675
AoxI GGCC 3 cut(s) 3, 81, 293
ApaLI GTGCAC 1 cut(s) 548
ApeKI GCWGC 3 cut(s) 701, 704, 707
AspS9I GGNCC 1 cut(s) 272
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 1 cut(s) 709
AvaII GGWCC 1 cut(s) 272
BaeGI GKGCMC 3 cut(s) 145, 388, 552
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 618
Bbv12I GWGCWC 3 cut(s) 256, 552, 582
BbvCI CCTCAGC 1 cut(s) 10
BbvI GCAGC 3 cut(s) 688, 691, 694
BccI CCATC 2 cut(s) 485, 491
BceAI ACGGC 2 cut(s) 17, 515
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BcnI CCSGG 1 cut(s) 618
BglI GCCNNNNNGGC 1 cut(s) 383
BisI GCNGC 8 cut(s) 169, 516, 603, 610, 702, 705, 708, 724
BlsI GCNGC 8 cut(s) 170, 517, 604, 611, 703, 706, 709, 725
BmcAI AGTACT 1 cut(s) 157
Bme1390I CCNGG 1 cut(s) 618
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 272
BmrFI CCNGG 1 cut(s) 618
BmsI GCATC 1 cut(s) 194
BoxI GACNNNNGTC 1 cut(s) 74
Bpu10I CCTNAGC 1 cut(s) 10
BpuMI CCSGG 1 cut(s) 618
BsaJI CCNNGG 2 cut(s) 166, 721
BsaWI WCCGGW 1 cut(s) 675
Bsc4I CCNNNNNNNGG 1 cut(s) 624
Bse1I ACTGG 4 cut(s) 136, 296, 549, 670
Bse3DI GCAATG 1 cut(s) 474
BseAI TCCGGA 1 cut(s) 675
BseDI CCNNGG 2 cut(s) 166, 721
BseLI CCNNNNNNNGG 1 cut(s) 624
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 2 cut(s) 24, 66
BseNI ACTGG 4 cut(s) 136, 296, 549, 670
BseSI GKGCMC 3 cut(s) 145, 388, 552
BseXI GCAGC 3 cut(s) 688, 691, 694
BseYI CCCAGC 1 cut(s) 405
Bsh1236I CGCG 2 cut(s) 168, 723
Bsh1285I CGRYCG 3 cut(s) 111, 510, 699
BshFI GGCC 3 cut(s) 5, 83, 295
BsiEI CGRYCG 3 cut(s) 111, 510, 699
BsiHKAI GWGCWC 3 cut(s) 256, 552, 582
BsiSI CCGG 2 cut(s) 618, 676
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 1 cut(s) 624
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 1 cut(s) 44
BsnI GGCC 3 cut(s) 5, 83, 295
Bsp1286I GDGCHC 6 cut(s) 145, 256, 388, 552, 582, 618
Bsp13I TCCGGA 1 cut(s) 675
Bsp143I GATC 4 cut(s) 108, 433, 456, 570
BspANI GGCC 3 cut(s) 5, 83, 295
BspCNI CTCAG 2 cut(s) 23, 65
BspEI TCCGGA 1 cut(s) 675
BspFNI CGCG 2 cut(s) 168, 723
BspHI TCATGA 1 cut(s) 330
BsrDI GCAATG 1 cut(s) 474
BsrI ACTGG 4 cut(s) 136, 296, 549, 670
BssECI CCNNGG 2 cut(s) 166, 721
BssMI GATC 4 cut(s) 108, 433, 456, 570
Bst4CI ACNGT 2 cut(s) 251, 398
BstC8I GCNNGC 2 cut(s) 607, 614
BstDEI CTNAG 2 cut(s) 10, 52
BstDSI CCRYGG 2 cut(s) 166, 721
BstFNI CGCG 2 cut(s) 168, 723
BstKTI GATC 4 cut(s) 111, 436, 459, 573
BstMBI GATC 4 cut(s) 108, 433, 456, 570
BstMCI CGRYCG 3 cut(s) 111, 510, 699
BstMWI GCNNNNNNNGC 7 cut(s) 11, 174, 383, 492, 512, 618, 707
BstNSI RCATGY 1 cut(s) 153
BstPAI GACNNNNGTC 1 cut(s) 74
BstSCI CCNGG 1 cut(s) 616
BstSLI GKGCMC 3 cut(s) 145, 388, 552
BstUI CGCG 2 cut(s) 168, 723
BstV1I GCAGC 3 cut(s) 688, 691, 694
BsuRI GGCC 3 cut(s) 5, 83, 295
BtgI CCRYGG 2 cut(s) 166, 721
BtgZI GCGATG 2 cut(s) 254, 510
BtsIMutI CAGTG 3 cut(s) 60, 143, 403
Cac8I GCNNGC 2 cut(s) 607, 614
CciI TCATGA 1 cut(s) 330
Cfr13I GGNCC 1 cut(s) 272
Cfr42I CCGCGG 2 cut(s) 169, 724
CseI GACGC 1 cut(s) 390
Csp6I GTAC 4 cut(s) 133, 156, 643, 667
CviAII CATG 4 cut(s) 150, 258, 331, 574
CviQI GTAC 4 cut(s) 133, 156, 643, 667
DdeI CTNAG 2 cut(s) 10, 52
DpnI GATC 4 cut(s) 110, 435, 458, 572
DpnII GATC 4 cut(s) 108, 433, 456, 570
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 2 cut(s) 264, 533
Eco24I GRGCYC 1 cut(s) 618
Eco47I GGWCC 1 cut(s) 272
Eco57I CTGAAG 1 cut(s) 510
EcoT38I GRGCYC 1 cut(s) 618
FaeI CATG 4 cut(s) 153, 261, 334, 577
FaqI GGGAC 1 cut(s) 388
FatI CATG 4 cut(s) 149, 257, 330, 573
FauI CCCGC 2 cut(s) 107, 728
FauNDI CATATG 1 cut(s) 220
Fnu4HI GCNGC 8 cut(s) 169, 516, 603, 610, 702, 705, 708, 724
FriOI GRGCYC 1 cut(s) 618
Fsp4HI GCNGC 8 cut(s) 169, 516, 603, 610, 702, 705, 708, 724
GluI GCNGC 8 cut(s) 169, 516, 603, 610, 702, 705, 708, 724
GsaI CCCAGC 1 cut(s) 409
HaeIII GGCC 3 cut(s) 5, 83, 295
HapII CCGG 2 cut(s) 618, 676
HgaI GACGC 1 cut(s) 390
Hin1II CATG 4 cut(s) 153, 261, 334, 577
HincII GTYRAC 1 cut(s) 693
HindII GTYRAC 1 cut(s) 693
HinfI GANTC 3 cut(s) 473, 584, 685
HpaII CCGG 2 cut(s) 618, 676
HphI GGTGA 1 cut(s) 709
Hpy166II GTNNAC 3 cut(s) 550, 636, 693
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 4 cut(s) 125, 331, 676, 765
Hpy8I GTNNAC 3 cut(s) 550, 636, 693
Hpy99I CGWCG 2 cut(s) 109, 602
HpyAV CCTTC 1 cut(s) 276
HpyCH4III ACNGT 2 cut(s) 251, 398
HpyCH4IV ACGT 1 cut(s) 657
HpyCH4V TGCA 6 cut(s) 44, 153, 195, 261, 535, 550
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 174, 383, 492, 512, 618, 707
HpyF3I CTNAG 2 cut(s) 10, 52
HpySE526I ACGT 1 cut(s) 657
Hsp92II CATG 4 cut(s) 153, 261, 334, 577
Kpn2I TCCGGA 1 cut(s) 675
KspI CCGCGG 2 cut(s) 169, 724
Kzo9I GATC 4 cut(s) 108, 433, 456, 570
LmnI GCTCC 2 cut(s) 259, 414
Lsp1109I GCAGC 3 cut(s) 688, 691, 694
LweI GCATC 1 cut(s) 194
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 1 cut(s) 161
MalI GATC 4 cut(s) 110, 435, 458, 572
MboI GATC 4 cut(s) 108, 433, 456, 570
MboII GAAGA 4 cut(s) 40, 300, 472, 664
MfeI CAATTG 1 cut(s) 72
MhlI GDGCHC 6 cut(s) 145, 256, 388, 552, 582, 618
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 72, 367, 712
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 482, 578
MmeI TCCRAC 2 cut(s) 225, 582
MnlI CCTC 7 cut(s) 19, 47, 203, 306, 312, 451, 556
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 675
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 545
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 4 cut(s) 168, 609, 701, 723
MspI CCGG 2 cut(s) 618, 676
MspR9I CCNGG 1 cut(s) 618
MunI CAATTG 1 cut(s) 72
Mva1269I GAATGC 1 cut(s) 44
MvnI CGCG 2 cut(s) 168, 723
MwoI GCNNNNNNNGC 7 cut(s) 11, 174, 383, 492, 512, 618, 707
NciI CCSGG 1 cut(s) 618
NdeI CATATG 1 cut(s) 220
NdeII GATC 4 cut(s) 108, 433, 456, 570
NlaIII CATG 4 cut(s) 153, 261, 334, 577
NspI RCATGY 1 cut(s) 153
PagI TCATGA 1 cut(s) 330
PctI GAATGC 1 cut(s) 44
PfeI GAWTC 1 cut(s) 685
PkrI GCNGC 8 cut(s) 170, 517, 604, 611, 703, 706, 709, 725
Ple19I CGATCG 1 cut(s) 111
PleI GAGTC 2 cut(s) 481, 578
PpsI GAGTC 2 cut(s) 481, 578
PshAI GACNNNNGTC 1 cut(s) 74
PspFI CCCAGC 1 cut(s) 405
PspPI GGNCC 1 cut(s) 272
PvuI CGATCG 1 cut(s) 111
RsaI GTAC 4 cut(s) 134, 157, 644, 668
RsaNI GTAC 4 cut(s) 133, 156, 643, 667
RseI CAYNNNNRTG 1 cut(s) 545
SacII CCGCGG 2 cut(s) 169, 724
SatI GCNGC 8 cut(s) 169, 516, 603, 610, 702, 705, 708, 724
Sau3AI GATC 4 cut(s) 108, 433, 456, 570
Sau96I GGNCC 1 cut(s) 272
ScaI AGTACT 1 cut(s) 157
SchI GAGTC 2 cut(s) 482, 578
ScrFI CCNGG 1 cut(s) 618
SduI GDGCHC 6 cut(s) 145, 256, 388, 552, 582, 618
SfaNI GCATC 1 cut(s) 194
Sfr303I CCGCGG 2 cut(s) 169, 724
SgrBI CCGCGG 2 cut(s) 169, 724
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 545
Sse9I AATT 3 cut(s) 72, 367, 712
StyD4I CCNGG 1 cut(s) 616
TaaI ACNGT 2 cut(s) 251, 398
TaiI ACGT 1 cut(s) 660
TaqI TCGA 3 cut(s) 298, 436, 582
TasI AATT 3 cut(s) 72, 367, 712
TatI WGTACW 2 cut(s) 155, 642
TauI GCSGC 5 cut(s) 171, 518, 605, 612, 726
TfiI GAWTC 1 cut(s) 685
TscAI CASTG 3 cut(s) 60, 143, 403
TseI GCWGC 3 cut(s) 701, 704, 707
TspGWI ACGGA 2 cut(s) 653, 678
TspRI CASTG 3 cut(s) 60, 143, 403
VneI GTGCAC 1 cut(s) 548
VpaK11BI GGWCC 1 cut(s) 272
XceI RCATGY 1 cut(s) 153
XcmI CCANNNNNNNNNTGG 2 cut(s) 484, 677
ZrmI AGTACT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.