RLG00000029763

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005550
Physical Location & Seq
Reverse (-)
48736323 .. 48736776
454 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029763

Sequence Viewer

Length: 423 bp
ATGATGCTCTCAGATGGTAAGGATGGGGCTGACCGAGTGTTGGCCGTTGTTGAGGAGACAGATCCACTCGATCCCAATCGGATCCGTTCTGATCTGGGGTTGAGTGTGTTGGATGTTGGAATAGGCAGCATGGCTTGGCAGGGGATGTCAGGGGACAGGTTGACTGGAGGGATGAGGTGGAGGATTTGCGTCGTTGTGGCCATGGCGTCGTTCTGTGACTCTGGTGATTCGGTGTCTCCACTGGTACTTCGGTTTCTAGGGGGCAAATTGGGCGACAATCTGACTGTTATGGTGGCTGGGCTCTGGACGGAGATGGGGTCACGGCAGAGGCGCGGTGATGGTGGCGTTATGGTGGCGGCGGTGTTGAACCCTGCATATTCCTTGCTGGGCCTGGTGCTTCTTGGGCCTGGGCTTGGGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

14.65

Weight (kDa)

4.76

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 333
AciI CCGC 3 cut(s) 333, 356, 359
AclWI GGATC 4 cut(s) 56, 65, 76, 89
AcoI YGGCCR 2 cut(s) 42, 198
AcyI GRCGYC 1 cut(s) 206
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 2 cut(s) 40, 413
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 2 cut(s) 390, 406
Alw26I GTCTC 2 cut(s) 50, 240
AlwI GGATC 4 cut(s) 56, 65, 76, 89
AoxI GGCC 4 cut(s) 42, 198, 388, 404
ApeKI GCWGC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 333
AspS9I GGNCC 2 cut(s) 388, 404
AsuHPI GGTGA 2 cut(s) 236, 347
BaeI ACNNNNGTAYC 2 cut(s) 236, 269
BalI TGGCCA 1 cut(s) 200
BamHI GGATCC 1 cut(s) 81
BanII GRGCYC 2 cut(s) 303, 420
BbvI GCAGC 1 cut(s) 138
BccI CCATC 4 cut(s) 8, 17, 307, 332
BceAI ACGGC 2 cut(s) 29, 338
BciT130I CCWGG 2 cut(s) 392, 408
BcoDI GTCTC 2 cut(s) 50, 240
BfaI CTAG 2 cut(s) 257, 421
BisI GCNGC 2 cut(s) 127, 357
BlsI GCNGC 2 cut(s) 128, 358
Bme1390I CCNGG 2 cut(s) 392, 408
BmgT120I GGNCC 2 cut(s) 388, 404
BmiI GGNNCC 1 cut(s) 83
BmrFI CCNGG 2 cut(s) 392, 408
BpmI CTGGAG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 75
BsaHI GRCGYC 1 cut(s) 206
BsaJI CCNNGG 2 cut(s) 201, 407
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 413
Bse1I ACTGG 2 cut(s) 169, 246
Bse8I GATNNNNATC 1 cut(s) 75
BseBI CCWGG 2 cut(s) 392, 408
BseDI CCNNGG 2 cut(s) 201, 407
BseGI GGATG 4 cut(s) 28, 118, 150, 177
BseJI GATNNNNATC 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 40, 413
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 2 cut(s) 169, 246
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 1 cut(s) 138
BseYI CCCAGC 2 cut(s) 296, 385
Bsh1236I CGCG 1 cut(s) 333
BshFI GGCC 4 cut(s) 44, 200, 390, 406
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 2 cut(s) 40, 413
BsmAI GTCTC 2 cut(s) 50, 240
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 4 cut(s) 44, 200, 390, 406
Bsp1286I GDGCHC 2 cut(s) 303, 420
Bsp143I GATC 4 cut(s) 61, 70, 81, 91
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 3 cut(s) 333, 356, 359
BspANI GGCC 4 cut(s) 44, 200, 390, 406
BspCNI CTCAG 1 cut(s) 23
BspFNI CGCG 1 cut(s) 333
BspLI GGNNCC 1 cut(s) 83
BspPI GGATC 4 cut(s) 56, 65, 76, 89
BsrI ACTGG 2 cut(s) 169, 246
BssECI CCNNGG 2 cut(s) 201, 407
BssMI GATC 4 cut(s) 61, 70, 81, 91
BssNI GRCGYC 1 cut(s) 206
BssT1I CCWWGG 1 cut(s) 201
Bst2UI CCWGG 2 cut(s) 392, 408
Bst4CI ACNGT 1 cut(s) 286
BstACI GRCGYC 1 cut(s) 206
BstDEI CTNAG 1 cut(s) 10
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 4 cut(s) 28, 118, 150, 177
BstFNI CGCG 1 cut(s) 333
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 4 cut(s) 64, 73, 84, 94
BstMAI GTCTC 2 cut(s) 50, 240
BstMBI GATC 4 cut(s) 61, 70, 81, 91
BstMWI GCNNNNNNNGC 2 cut(s) 270, 403
BstNI CCWGG 2 cut(s) 392, 408
BstSCI CCNGG 2 cut(s) 390, 406
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 1 cut(s) 138
BstX2I RGATCY 2 cut(s) 61, 81
BstYI RGATCY 2 cut(s) 61, 81
BsuRI GGCC 4 cut(s) 44, 200, 390, 406
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 4 cut(s) 28, 118, 150, 177
BtsIMutI CAGTG 1 cut(s) 239
CfoI GCGC 1 cut(s) 333
Cfr13I GGNCC 2 cut(s) 388, 404
CseI GACGC 2 cut(s) 178, 195
Csp6I GTAC 1 cut(s) 245
CviAII CATG 2 cut(s) 130, 202
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 1 cut(s) 10
DpnI GATC 4 cut(s) 63, 72, 83, 93
DpnII GATC 4 cut(s) 61, 70, 81, 91
EaeI YGGCCR 2 cut(s) 42, 198
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 2 cut(s) 303, 420
EcoRII CCWGG 2 cut(s) 390, 406
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 2 cut(s) 303, 420
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 2 cut(s) 133, 205
FaiI YATR 5 cut(s) 131, 203, 290, 350, 376
FaqI GGGAC 1 cut(s) 167
FatI CATG 2 cut(s) 129, 201
Fnu4HI GCNGC 2 cut(s) 127, 357
FokI GGATG 4 cut(s) 35, 125, 157, 184
FriOI GRGCYC 2 cut(s) 303, 420
Fsp4HI GCNGC 2 cut(s) 127, 357
FspBI CTAG 2 cut(s) 257, 421
GlaI GCGC 1 cut(s) 332
GluI GCNGC 2 cut(s) 127, 357
GsaI CCCAGC 2 cut(s) 300, 389
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 4 cut(s) 44, 200, 390, 406
HgaI GACGC 2 cut(s) 178, 195
HhaI GCGC 1 cut(s) 333
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 2 cut(s) 133, 205
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HinfI GANTC 2 cut(s) 218, 227
HphI GGTGA 2 cut(s) 236, 347
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 4 cut(s) 13, 81, 91, 282
Hpy188III TCNNGA 1 cut(s) 304
Hpy8I GTNNAC 1 cut(s) 162
Hpy99I CGWCG 2 cut(s) 194, 211
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 1 cut(s) 374
HpyF10VI GCNNNNNNNGC 2 cut(s) 270, 403
HpyF3I CTNAG 1 cut(s) 10
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 2 cut(s) 133, 205
HspAI GCGC 1 cut(s) 331
Kzo9I GATC 4 cut(s) 61, 70, 81, 91
Lsp1109I GCAGC 1 cut(s) 138
MaeI CTAG 2 cut(s) 257, 421
MaeIII GTNAC 2 cut(s) 215, 318
MalI GATC 4 cut(s) 63, 72, 83, 93
MboI GATC 4 cut(s) 61, 70, 81, 91
MflI RGATCY 2 cut(s) 61, 81
MhlI GDGCHC 2 cut(s) 303, 420
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 1 cut(s) 266
MluNI TGGCCA 1 cut(s) 200
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 2 cut(s) 90, 97
MnlI CCTC 5 cut(s) 46, 161, 168, 174, 321
Mox20I TGGCCA 1 cut(s) 200
MscI TGGCCA 1 cut(s) 200
Msp20I TGGCCA 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 392, 408
MvaI CCWGG 2 cut(s) 392, 408
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 2 cut(s) 270, 403
NcoI CCATGG 1 cut(s) 201
NdeII GATC 4 cut(s) 61, 70, 81, 91
NlaIII CATG 2 cut(s) 133, 205
NlaIV GGNNCC 1 cut(s) 83
NmuCI GTSAC 2 cut(s) 215, 318
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 128, 358
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp6I CCWGG 2 cut(s) 390, 406
PspFI CCCAGC 2 cut(s) 296, 385
PspGI CCWGG 2 cut(s) 390, 406
PspN4I GGNNCC 1 cut(s) 83
PspPI GGNCC 2 cut(s) 388, 404
PsuI RGATCY 2 cut(s) 61, 81
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SatI GCNGC 2 cut(s) 127, 357
Sau3AI GATC 4 cut(s) 61, 70, 81, 91
Sau96I GGNCC 2 cut(s) 388, 404
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 392, 408
SduI GDGCHC 2 cut(s) 303, 420
SetI ASST 2 cut(s) 161, 179
Sse9I AATT 1 cut(s) 266
SsiI CCGC 3 cut(s) 333, 356, 359
SspMI CTAG 2 cut(s) 257, 421
StyD4I CCNGG 2 cut(s) 390, 406
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 1 cut(s) 69
TaqII GACCGA 1 cut(s) 48
TasI AATT 1 cut(s) 266
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 1 cut(s) 227
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 2 cut(s) 215, 318
TseI GCWGC 1 cut(s) 126
Tsp45I GTSAC 2 cut(s) 215, 318
TspGWI ACGGA 2 cut(s) 74, 323
TspRI CASTG 1 cut(s) 246
XspI CTAG 2 cut(s) 257, 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.