Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0002185

Physical Location & Seq

Genomic Coordinates Reverse (-)

655010 .. 662985

Gene Structure 7976 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3198 bp

ATGGGAGTTCCGGCGTTCTACCGGTGGTTGGCGGAGAAGTACCCGCTCGTTATTGTCGACGTCGTGGAAGAAGAGCCCGTCGTGATAGATGACGTATCGATTCCGGTCGATACCAGTAAACCAAACCCTAATGGGTTGGAATACGATAATCTGTATCTCGACATGAACGGGATTATTCACCCCTGTTTCCACCCAGAAGATAGGCCTTCTCCCTCGACATTCGATGAGGTATTCCAGTGCATGTTTGATTATATTGACAGACTGTTTGTGATGGTTTGGCCTCGAAAGCTATTGTACATGGCTATTGATGGTGTTGCCCCCAGGGCAAAAATGAATCAACAGCGGTCTCGGCGATTTAGAGCTGCGAAAGATGCATCAGACGCGGCAGCTGAAGAAGAAAGGCTGCGTGAGGAGTTTGAAATGGAGGGGAGAAAGCTTCCTCCAAAACAACAGTCACAAGTTGTTGATTCCAATGTCATCACTCCTGGAACTCAATTTATGGCTGTTTTGTCAGTAGCATTGCAGTATTACATTCATCTCAGATTAAACAACGACCCAGGATGGAAAAATGTTAAGGTTATTCTTTCCGATGCAAATGTTCCAGGTGAAGGTGAACACAAAATTATGTCTTATGTTCGTCTTCAAAGAAATCTTCCTGGTTATGATCCAAATACACGCCATTGTCTCTATGGTTTGGATGCTGATTTGATTATGCTTGCTTTGGCTACCCATGAAATTCATTTTTCAATTCTTCGAGAGGTTGTATTCACCCCCGGACAGCAAGACAAATGCTTCATATGTGGTCAGGTGGGTCATTTAGCAGCAAATTGTGAAGGTAAGGTGAAACGGAAGGCTGGAGAGTTTGATGAGAAAGCTGATGCTGAGGCCGTTCCCAAGAAGCCGTACCAGTTTCTTAACATCTGGACTCTTAGAGAATATTTGGAATATGAGATGAGAATTCCCAATACCCAATTCGAGATTGATTTTGAGTGCATCGTGGATGATTTCATATTCATGTGTTTCTTCGTTGGCAACGATTTCTTACCACACATGCCCACGCTGGAAATTCGTGAGGGTGCAATCAATTTGCTTTTGGCTGTATACAAGAAAGAATTTAAGTCATTGGGTGGATACCTGACTGATGGAAGCAAGCCAAATTTGAGTCGGGTAGAGCATTTCATCCAAGCAGTTGGATCTTATGAAGATAAAATATTTCAGAAAAGAGCTCGATTGCATCAGCGTCAGGCAGACAGAATAAAGCGTGAAAAAGGGCAATTAAGAAGAGGAGATGATGCAGCGCCTCAAGTGCAACCAGATTCATTGGTTCCAGTTTCTCGGTTTCAGGGATCTCGTCTTGCTTCAGGTCATACAGCTTCACCCTATCAACAATCTGGAAAATCTAGTGATCAAGGAAATCCTAGAAAGGTTGCACGTTTGTCTTCAGAGATGTCAACTGTCGGTGCTGCTATTGTTGAAGCTGAGGACAGTCTTGAACTAGATGAACATGGAAACAAAGAAGACATGAAAGCAAAGCTGAAGGAGATTCTTCGTGAGAAATCTGATGCTTTTAACTCTGGAAATCCCAATGTAGACAAGGTTAAATTGGGAGAACGTGGATGGAAAGATAGGTATTATGAAGAGAAGTTTTCTGCAAAAACTCCTGAAGAACGTGATGCAATTCGAAAAGATGTTGTTTTGCGATACACTGAAGGTCTGTGTTGGGTGATGCACTATTATTATGAAGGTGTTTGCTCGTGGCAGTGGTTTTACCCCTATCACTATGCACCCTTTGCTTCTGATCTGAAGGACCTTGGTCAGCTAAATATAAGTTTTGATTTGGGCTCTCCTTTTAAACCGTTCAATCAGCTGTTGGGAGTTTTTCCTGCTGCAAGCTCCCATGCTCTTCCTGAGCAGTACAGGAAGTTGATGACTGATCCAAATTCACCTCTGATTGATTTTTACCCTATTGACTTTGAAGTGGATATGAATGGGAAGCGGTATGCATGGCAGGGTATTGCAAAGTTGCCTTTTATTGATGAAGTCCGCCTTCTAGGTGAGGTTGAAAAACTCGAACATACATTGACGGAGGAGGAGACAAGGAGAAATAGTGTGATGTTTGAGATGCTTTTTGTGACATCGTCTCATCCTCTATCTGTAAGCATATATTCTCTGGACAATCGATGTAAGCAGTTGACAGACAAAGAACGAGTTGAAGTTAAGGAGCAGATTGATCCCAAGTACAGTGAAGGGATGAACGGTTATTTGTCTCCATGTACTGGAGAGCCTTGCCCTCCGGTTTTCCGGTCTCCTGTTGAAAGTATGGAGGATATTGTGGACAACCAAGTAATATGTGCGATATACAGACTACCGGATGTACATAAGCATATAACTCAACCACCGGCAGGTGTTATATTCCCAAAGAAGACTGTTACAGTGGGAGATCTGAAGCCTTTACCTCCTTTATGGCATGAAGACACCGGGCGAAAGCCTTGGCATGAAGATTCTGGGCGGAAGCCATATGAAAATTCGAGTTCCAGGCAGAACCCAGGAGCTATTTCTGGAAGACAGCTTGGGGAATCGGCACATCGCCTTGTTGTTAATAGCTTACAGGCTAATGGGTATGGCAATCGGATGCATTCCCATCAACCATCTTATGCCGAGCCTCAGTATTATCCAGCACACCCTTCCTATGCAAATAACGGAGGCTATGATCAGGGACACTATAGAATGCCACCACCTGGTGCAATCCACCATCCTCAACATCAACACCGAGCTTCGAATATTAGTACATTCCCAAGTCACGGTGACCATGGGTATAATGTACCGCATTCTTCGGCAGCTGGCCATCGCTTTCAGAACAGGTCTCGTACCCAGCACCACGAAAGGAATTCTGGGCATGTATACTACGAATCAGGAAATCACCAGAACGATGGGCCAATCTACCCTGAAAGACCAATTCTACAAACTCCAGCGGGGGTCAGTGCTGCTCCTTATCCAGGTGGCTATAACAACTGTCAGAGTTACCAACCATCTGGAACGCCTAGCCATCATGGTATTGATGGCCGGGTTCCACCGGTCAGCCATAACCCGGGCAGAGGACAAAGCCGCCCTCAACAATCCAGAAACCAATACTCTGGATTGGACAGAAGAGGAAATAGGAGACCACCTCCACCCCAGGCTGGGTATGGTCACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000966 GO:0000967 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004534 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008409 GO:0009056 GO:0009057 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010586 GO:0010587 GO:0010604 GO:0010628 GO:0016070 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019222 GO:0019439 GO:0022613 GO:0034470 GO:0034471 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060147 GO:0060149 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0071025 GO:0071027 GO:0071029 GO:0071035 GO:0071046 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03008 ko03018 map03008 map03018

Pfam Domains

Protein Families

XRN_N zf-CCHC

Protein Analysis

1066

Amino Acids

121.06

Weight (kDa)

6.43

Isoelectric Point (pI)

49.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
XRN_N	PF03159	1 - 253	4.3e-100	XRN 5'-3' exonuclease N-terminus
XRN_M	PF17846	327 - 814	1.5e-165	Xrn1 helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002185)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75660 AT5G42540 AT5G42540
fragaria_vesca	FvH4_2g38700 FvH4_2g38700 FvH4_5g21470
malus_domestica	MD00G1128500.v1.1 MD08G1014700.v1.1 MD15G1014000.v1.1
prunus_persica	Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1
pyrus_communis	pycom08g01310 pycom15g01210
rosa_chinensis	RchiOBHm_Chr5g0009961 RchiOBHm_Chr6g0302611
rosa_laevigata	RLG00000011157 RLG00000031714
rosa_multiflora	Rmu_sc0027322.1_g000001 Rmu_sc0027323.1_g000001 Rmu_sc0039467.1_g000001 Rmu_sc0039989.1_g000001 Rmu_sc0041627.1_g000001
rosa_roxburghii	Rroxscaffold_1G00066230 Rroxscaffold_7G00165520
rosa_rugosa	Rorug04G0447300 Rorug06G0315300 Rorug06G0315400
rosa_samantha	Rh5AG078800 Rh5BG073500 Rh5CG086100 Rh5DG074200 Rh5DG336700 Rh6AG428700 Rh6BG473800 Rh6CG441000 Rh6DG427700
rosa_wichuraiana	Rw6G037100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2393
AatII	GACGTC	1 cut(s)	63
Acc36I	ACCTGC	1 cut(s)	2393
AccB7I	CCANNNNNTGG	2 cut(s)	2276, 2738
AccBSI	CCGCTC	1 cut(s)	46
AccI	GTMKAC	4 cut(s)	57, 1101, 1590, 2901
AccII	CGCG	1 cut(s)	383
AclWI	GGATC	5 cut(s)	659, 1201, 1354, 1928, 2225
AcoI	YGGCCR	2 cut(s)	2842, 3061
AcsI	RAATTY	8 cut(s)	735, 957, 1065, 1112, 1156, 1939, 2524, 2887
AcuI	CTGAAG	8 cut(s)	411, 1344, 1425, 1556, 1683, 1728, 1823, 2465
AcyI	GRCGYC	1 cut(s)	60
AdeI	CACNNNGTG	1 cut(s)	2741
AgeI	ACCGGT	2 cut(s)	21, 3073
AjuI	GAANNNNNNNTTGG	2 cut(s)	956, 988
Alw21I	GWGCWC	1 cut(s)	1228
Alw26I	GTCTC	8 cut(s)	351, 689, 2087, 2145, 2271, 2310, 2868, 3154
AlwI	GGATC	5 cut(s)	659, 1201, 1354, 1928, 2225
Ama87I	CYCGRG	1 cut(s)	3088
AoxI	GGCC	6 cut(s)	203, 278, 885, 2842, 2933, 3061
ApeKI	GCWGC	9 cut(s)	362, 386, 403, 821, 1295, 1463, 1886, 2837, 2984
ApoI	RAATTY	8 cut(s)	735, 957, 1065, 1112, 1156, 1939, 2524, 2887
ArsI	GACNNNNNNTTYG	4 cut(s)	248, 280, 437, 469
AsiGI	ACCGGT	2 cut(s)	21, 3073
AspLEI	GCGC	1 cut(s)	1300
AspS9I	GGNCC	2 cut(s)	1807, 2933
AsuC2I	CCSGG	5 cut(s)	774, 2479, 3065, 3089, 3090
AsuII	TTCGAA	2 cut(s)	1681, 2777
AvaI	CYCGRG	1 cut(s)	3088
AvaII	GGWCC	1 cut(s)	1807
BalI	TGGCCA	1 cut(s)	2844
BanII	GRGCYC	3 cut(s)	78, 1228, 1844
BauI	CACGAG	1 cut(s)	1753
BbsI	GAAGAC	6 cut(s)	632, 1431, 1524, 2429, 2478, 2569
Bbv12I	GWGCWC	1 cut(s)	1228
BbvCI	CCTCAGC	2 cut(s)	882, 1479
BbvI	GCAGC	9 cut(s)	349, 390, 398, 833, 1307, 1450, 1873, 2849, 2971
BceAI	ACGGC	2 cut(s)	872, 886
BciVI	GTATCC	1 cut(s)	1124
BclI	TGATCA	2 cut(s)	1405, 2710
BcnI	CCSGG	5 cut(s)	774, 2479, 3065, 3089, 3090
BcoDI	GTCTC	8 cut(s)	351, 689, 2087, 2145, 2271, 2310, 2868, 3154
BfaI	CTAG	5 cut(s)	1401, 1419, 1496, 2051, 3042
BfmI	CTRYAG	1 cut(s)	2722
BfoI	RGCGCY	1 cut(s)	1301
BfuAI	ACCTGC	1 cut(s)	2393
BfuI	GTATCC	1 cut(s)	1124
BglI	GCCNNNNNGGC	1 cut(s)	323
BglII	AGATCT	1 cut(s)	2440
Bme18I	GGWCC	1 cut(s)	1807
BmeT110I	CYCGRG	1 cut(s)	3088
BmgT120I	GGNCC	2 cut(s)	1807, 2933
BmiI	GGNNCC	2 cut(s)	1326, 3069
BoxI	GACNNNNGTC	1 cut(s)	1812
BpiI	GAAGAC	6 cut(s)	632, 1431, 1524, 2429, 2478, 2569
BplI	GAGNNNNNCTC	2 cut(s)	3151, 3183
BpmI	CTGGAG	3 cut(s)	876, 2298, 2952
Bpu10I	CCTNAGC	3 cut(s)	882, 1479, 1908
Bpu14I	TTCGAA	2 cut(s)	1681, 2777
BpuEI	CTTGAG	1 cut(s)	1287
BpuMI	CCSGG	5 cut(s)	774, 2479, 3065, 3089, 3090
Bsa29I	ATCGAT	2 cut(s)	98, 2179
BsaHI	GRCGYC	1 cut(s)	60
BsaI	GGTCTC	4 cut(s)	351, 2310, 2868, 3154
BsaWI	WCCGGW	6 cut(s)	21, 103, 2293, 2301, 2368, 3073
BsaXI	ACNNNNNCTCC	2 cut(s)	2092, 2122
Bse118I	RCCGGY	3 cut(s)	21, 2398, 3073
Bse1I	ACTGG	5 cut(s)	114, 235, 907, 1328, 2281
Bse3DI	GCAATG	1 cut(s)	518
BseCI	ATCGAT	2 cut(s)	98, 2179
BseMI	GCAATG	1 cut(s)	518
BseMII	CTCAG	5 cut(s)	553, 873, 1470, 1899, 2678
BseNI	ACTGG	5 cut(s)	114, 235, 907, 1328, 2281
BseRI	GAGGAG	4 cut(s)	425, 1299, 2102, 2105
BseXI	GCAGC	9 cut(s)	349, 390, 398, 833, 1307, 1450, 1873, 2849, 2971
BseYI	CCCAGC	2 cut(s)	2871, 3179
Bsh1236I	CGCG	1 cut(s)	383
Bsh1285I	CGRYCG	1 cut(s)	108
BshFI	GGCC	6 cut(s)	205, 280, 887, 2844, 2935, 3063
BshTI	ACCGGT	2 cut(s)	21, 3073
BshVI	ATCGAT	2 cut(s)	98, 2179
BsiEI	CGRYCG	1 cut(s)	108
BsiHKAI	GWGCWC	1 cut(s)	1228
BsiHKCI	CYCGRG	1 cut(s)	3088
BslFI	GGGAC	1 cut(s)	2730
BsmAI	GTCTC	8 cut(s)	351, 689, 2087, 2145, 2271, 2310, 2868, 3154
BsmBI	CGTCTC	1 cut(s)	2145
BsmFI	GGGAC	1 cut(s)	2730
BsmI	GAATGC	3 cut(s)	2635, 2733, 2827
BsnI	GGCC	6 cut(s)	205, 280, 887, 2844, 2935, 3063
Bso31I	GGTCTC	4 cut(s)	351, 2310, 2868, 3154
BsoBI	CYCGRG	1 cut(s)	3088
Bsp119I	TTCGAA	2 cut(s)	1681, 2777
Bsp1286I	GDGCHC	3 cut(s)	78, 1228, 1844
Bsp1407I	TGTACA	2 cut(s)	294, 2374
Bsp143I	GATC	9 cut(s)	664, 1193, 1346, 1405, 1798, 1933, 2230, 2440, 2710
Bsp19I	CCATGG	1 cut(s)	2809
BspANI	GGCC	6 cut(s)	205, 280, 887, 2844, 2935, 3063
BspCNI	CTCAG	5 cut(s)	552, 874, 1471, 1900, 2677
BspDI	ATCGAT	2 cut(s)	98, 2179
BspFNI	CGCG	1 cut(s)	383
BspLI	GGNNCC	2 cut(s)	1326, 3069
BspMI	ACCTGC	1 cut(s)	2393
BspPI	GGATC	5 cut(s)	659, 1201, 1354, 1928, 2225
BspQI	GCTCTTC	2 cut(s)	66, 1908
BspT104I	TTCGAA	2 cut(s)	1681, 2777
BspTNI	GGTCTC	4 cut(s)	351, 2310, 2868, 3154
BsrBI	CCGCTC	1 cut(s)	46
BsrDI	GCAATG	1 cut(s)	518
BsrFI	RCCGGY	3 cut(s)	21, 2398, 3073
BsrGI	TGTACA	2 cut(s)	294, 2374
BsrI	ACTGG	5 cut(s)	114, 235, 907, 1328, 2281
BssAI	RCCGGY	3 cut(s)	21, 2398, 3073
BssMI	GATC	9 cut(s)	664, 1193, 1346, 1405, 1798, 1933, 2230, 2440, 2710
BssNAI	GTATAC	2 cut(s)	1102, 2902
BssNI	GRCGYC	1 cut(s)	60
BssSI	CACGAG	1 cut(s)	1753
BssT1I	CCWWGG	3 cut(s)	1810, 2489, 2809
Bst1107I	GTATAC	2 cut(s)	1102, 2902
Bst2BI	CACGAG	1 cut(s)	1753
Bst6I	CTCTTC	5 cut(s)	66, 1276, 1632, 1908, 3142
BstACI	GRCGYC	1 cut(s)	60
BstAPI	GCANNNNNTGC	1 cut(s)	1790
BstAUI	TGTACA	2 cut(s)	294, 2374
BstBI	TTCGAA	2 cut(s)	1681, 2777
BstC8I	GCNNGC	4 cut(s)	717, 1151, 1891, 2842
BstDEI	CTNAG	6 cut(s)	539, 882, 929, 1479, 1908, 2664
BstDSI	CCRYGG	1 cut(s)	2809
BstEII	GGTNACC	1 cut(s)	2804
BstENI	CCTNNNNNAGG	1 cut(s)	2994
BstFNI	CGCG	1 cut(s)	383
BstH2I	RGCGCY	1 cut(s)	1301
BstHHI	GCGC	1 cut(s)	1300
BstKTI	GATC	9 cut(s)	667, 1196, 1349, 1408, 1801, 1936, 2233, 2443, 2713
BstMAI	GTCTC	8 cut(s)	351, 689, 2087, 2145, 2271, 2310, 2868, 3154
BstMBI	GATC	9 cut(s)	664, 1193, 1346, 1405, 1798, 1933, 2230, 2440, 2710
BstMCI	CGRYCG	1 cut(s)	108
BstMWI	GCNNNNNNNGC	7 cut(s)	286, 323, 349, 371, 380, 1306, 1790
BstNSI	RCATGY	3 cut(s)	244, 1054, 2900
BstPAI	GACNNNNGTC	1 cut(s)	1812
BstPI	GGTNACC	1 cut(s)	2804
BstSFI	CTRYAG	1 cut(s)	2722
BstUI	CGCG	1 cut(s)	383
BstV1I	GCAGC	9 cut(s)	349, 390, 398, 833, 1307, 1450, 1873, 2849, 2971
BstV2I	GAAGAC	6 cut(s)	632, 1431, 1524, 2429, 2478, 2569
BstX2I	RGATCY	3 cut(s)	1193, 1346, 2440
BstXI	CCANNNNNNTGG	4 cut(s)	1190, 2930, 3032, 3134
BstYI	RGATCY	3 cut(s)	1193, 1346, 2440
BstZ17I	GTATAC	2 cut(s)	1102, 2902
Bsu15I	ATCGAT	2 cut(s)	98, 2179
BsuI	GTATCC	1 cut(s)	1124
BsuRI	GGCC	6 cut(s)	205, 280, 887, 2844, 2935, 3063
BsuTUI	ATCGAT	2 cut(s)	98, 2179
BtgI	CCRYGG	1 cut(s)	2809
BtgZI	GCGATG	2 cut(s)	2570, 2831
BtsI	GCAGTG	1 cut(s)	1766
BtsIMutI	CAGTG	6 cut(s)	242, 1704, 1766, 2248, 2439, 2986
BveI	ACCTGC	1 cut(s)	2393
Cac8I	GCNNGC	4 cut(s)	717, 1151, 1891, 2842
CfoI	GCGC	1 cut(s)	1300
Cfr10I	RCCGGY	3 cut(s)	21, 2398, 3073
Cfr13I	GGNCC	2 cut(s)	1807, 2933
Cfr9I	CCCGGG	1 cut(s)	3088
ClaI	ATCGAT	2 cut(s)	98, 2179
CseI	GACGC	2 cut(s)	389, 1229
CsiI	ACCWGGT	1 cut(s)	2737
CspAI	ACCGGT	2 cut(s)	21, 3073
DdeI	CTNAG	6 cut(s)	539, 882, 929, 1479, 1908, 2664
DpnI	GATC	9 cut(s)	666, 1195, 1348, 1407, 1800, 1935, 2232, 2442, 2712
DpnII	GATC	9 cut(s)	664, 1193, 1346, 1405, 1798, 1933, 2230, 2440, 2710
DraI	TTTAAA	1 cut(s)	1852
DraIII	CACNNNGTG	1 cut(s)	2741
EaeI	YGGCCR	2 cut(s)	2842, 3061
Eam1104I	CTCTTC	5 cut(s)	66, 1276, 1632, 1908, 3142
EarI	CTCTTC	5 cut(s)	66, 1276, 1632, 1908, 3142
EciI	GGCGGA	3 cut(s)	47, 2033, 2524
Ecl136II	GAGCTC	1 cut(s)	1226
Eco130I	CCWWGG	3 cut(s)	1810, 2489, 2809
Eco147I	AGGCCT	1 cut(s)	205
Eco24I	GRGCYC	3 cut(s)	78, 1228, 1844
Eco31I	GGTCTC	4 cut(s)	351, 2310, 2868, 3154
Eco47I	GGWCC	1 cut(s)	1807
Eco53kI	GAGCTC	1 cut(s)	1226
Eco57I	CTGAAG	8 cut(s)	411, 1344, 1425, 1556, 1683, 1728, 1823, 2465
Eco88I	CYCGRG	1 cut(s)	3088
Eco91I	GGTNACC	1 cut(s)	2804
EcoICRI	GAGCTC	1 cut(s)	1226
EcoNI	CCTNNNNNAGG	1 cut(s)	2994
EcoO109I	RGGNCCY	1 cut(s)	1807
EcoO65I	GGTNACC	1 cut(s)	2804
EcoRI	GAATTC	2 cut(s)	957, 2887
EcoT14I	CCWWGG	3 cut(s)	1810, 2489, 2809
EcoT22I	ATGCAT	3 cut(s)	376, 2005, 2637
EcoT38I	GRGCYC	3 cut(s)	78, 1228, 1844
ErhI	CCWWGG	3 cut(s)	1810, 2489, 2809
Esp3I	CGTCTC	1 cut(s)	2145
FalI	AAGNNNNNCTT	2 cut(s)	2031, 2063
FaqI	GGGAC	1 cut(s)	2730
FauI	CCCGC	2 cut(s)	51, 2965
FauNDI	CATATG	2 cut(s)	797, 2518
FbaI	TGATCA	2 cut(s)	1405, 2710
FblI	GTMKAC	4 cut(s)	57, 1101, 1590, 2901
FriOI	GRGCYC	3 cut(s)	78, 1228, 1844
FspBI	CTAG	5 cut(s)	1401, 1419, 1496, 2051, 3042
GlaI	GCGC	1 cut(s)	1299
GsaI	CCCAGC	2 cut(s)	2875, 3183
GsuI	CTGGAG	3 cut(s)	876, 2298, 2952
HaeII	RGCGCY	1 cut(s)	1301
HaeIII	GGCC	6 cut(s)	205, 280, 887, 2844, 2935, 3063
HgaI	GACGC	2 cut(s)	389, 1229
HhaI	GCGC	1 cut(s)	1300
Hin1I	GRCGYC	1 cut(s)	60
Hin6I	GCGC	1 cut(s)	1298
HinP1I	GCGC	1 cut(s)	1298
HincII	GTYRAC	3 cut(s)	58, 1452, 2193
HindII	GTYRAC	3 cut(s)	58, 1452, 2193
HindIII	AAGCTT	1 cut(s)	434
Hpy166II	GTNNAC	9 cut(s)	58, 119, 614, 1102, 1452, 1591, 2193, 2335, 2902
Hpy8I	GTNNAC	9 cut(s)	58, 119, 614, 1102, 1452, 1591, 2193, 2335, 2902
Hpy99I	CGWCG	3 cut(s)	62, 65, 83
HpyCH4IV	ACGT	5 cut(s)	60, 93, 1432, 1612, 1669
HpyF10VI	GCNNNNNNNGC	7 cut(s)	286, 323, 349, 371, 380, 1306, 1790
HpyF3I	CTNAG	6 cut(s)	539, 882, 929, 1479, 1908, 2664
HpySE526I	ACGT	5 cut(s)	60, 93, 1432, 1612, 1669
Hsp92I	GRCGYC	1 cut(s)	60
HspAI	GCGC	1 cut(s)	1298
Ksp22I	TGATCA	2 cut(s)	1405, 2710
Kzo9I	GATC	9 cut(s)	664, 1193, 1346, 1405, 1798, 1933, 2230, 2440, 2710
LguI	GCTCTTC	2 cut(s)	66, 1908
LmnI	GCTCC	4 cut(s)	1898, 2221, 2549, 2992
Lsp1109I	GCAGC	9 cut(s)	349, 390, 398, 833, 1307, 1450, 1873, 2849, 2971
MabI	ACCWGGT	1 cut(s)	2737
MaeI	CTAG	5 cut(s)	1401, 1419, 1496, 2051, 3042
MaeII	ACGT	5 cut(s)	60, 93, 1432, 1612, 1669
MaeIII	GTNAC	7 cut(s)	453, 2131, 2428, 2798, 2804, 3020, 3188
MalI	GATC	9 cut(s)	666, 1195, 1348, 1407, 1800, 1935, 2232, 2442, 2712
MbiI	CCGCTC	1 cut(s)	46
MboI	GATC	9 cut(s)	664, 1193, 1346, 1405, 1798, 1933, 2230, 2440, 2710
MflI	RGATCY	3 cut(s)	1193, 1346, 2440
MhlI	GDGCHC	3 cut(s)	78, 1228, 1844
MlsI	TGGCCA	1 cut(s)	2844
MluNI	TGGCCA	1 cut(s)	2844
MlyI	GAGTC	2 cut(s)	919, 1171
MmeI	TCCRAC	2 cut(s)	117, 1171
Mox20I	TGGCCA	1 cut(s)	2844
Mph1103I	ATGCAT	3 cut(s)	376, 2005, 2637
MscI	TGGCCA	1 cut(s)	2844
MslI	CAYNNNNRTG	1 cut(s)	1013
Msp20I	TGGCCA	1 cut(s)	2844
MspA1I	CMGCKG	5 cut(s)	343, 389, 1867, 2840, 2972
Mva1269I	GAATGC	3 cut(s)	2635, 2733, 2827
MvnI	CGCG	1 cut(s)	383
MwoI	GCNNNNNNNGC	7 cut(s)	286, 323, 349, 371, 380, 1306, 1790
NciI	CCSGG	5 cut(s)	774, 2479, 3065, 3089, 3090
NcoI	CCATGG	1 cut(s)	2809
NdeI	CATATG	2 cut(s)	797, 2518
NdeII	GATC	9 cut(s)	664, 1193, 1346, 1405, 1798, 1933, 2230, 2440, 2710
NlaIV	GGNNCC	2 cut(s)	1326, 3069
NmeAIII	GCCGAG	2 cut(s)	328, 2683
NmuCI	GTSAC	5 cut(s)	453, 2131, 2798, 2804, 3188
NsiI	ATGCAT	3 cut(s)	376, 2005, 2637
NspI	RCATGY	3 cut(s)	244, 1054, 2900
NspV	TTCGAA	2 cut(s)	1681, 2777
PaqCI	CACCTGC	1 cut(s)	2393
PasI	CCCWGGG	1 cut(s)	321
PceI	AGGCCT	1 cut(s)	205
PciSI	GCTCTTC	2 cut(s)	66, 1908
PcsI	WCGNNNNNNNCGW	2 cut(s)	54, 1032
PctI	GAATGC	3 cut(s)	2635, 2733, 2827
PfeI	GAWTC	8 cut(s)	100, 334, 467, 1316, 1543, 2501, 2576, 2909
PflFI	GACNNNGTC	1 cut(s)	2137
PflMI	CCANNNNNTGG	2 cut(s)	2276, 2738
PfoI	TCCNGGA	1 cut(s)	484
PinAI	ACCGGT	2 cut(s)	21, 3073
PleI	GAGTC	2 cut(s)	919, 1170
PpsI	GAGTC	2 cut(s)	919, 1170
PpuMI	RGGWCCY	1 cut(s)	1807
PshAI	GACNNNNGTC	1 cut(s)	1812
Psp124BI	GAGCTC	1 cut(s)	1228
Psp5II	RGGWCCY	1 cut(s)	1807
PspEI	GGTNACC	1 cut(s)	2804
PspFI	CCCAGC	2 cut(s)	2871, 3179
PspN4I	GGNNCC	2 cut(s)	1326, 3069
PspPI	GGNCC	2 cut(s)	1807, 2933
PspPPI	RGGWCCY	1 cut(s)	1807
PsuI	RGATCY	3 cut(s)	1193, 1346, 2440
PsyI	GACNNNGTC	1 cut(s)	2137
PvuII	CAGCTG	3 cut(s)	389, 1867, 2840
RseI	CAYNNNNRTG	1 cut(s)	1013
SacI	GAGCTC	1 cut(s)	1228
SalI	GTCGAC	1 cut(s)	56
SapI	GCTCTTC	2 cut(s)	66, 1908
Sau3AI	GATC	9 cut(s)	664, 1193, 1346, 1405, 1798, 1933, 2230, 2440, 2710
Sau96I	GGNCC	2 cut(s)	1807, 2933
SchI	GAGTC	2 cut(s)	919, 1171
SduI	GDGCHC	3 cut(s)	78, 1228, 1844
SexAI	ACCWGGT	1 cut(s)	2737
SfcI	CTRYAG	1 cut(s)	2722
SfuI	TTCGAA	2 cut(s)	1681, 2777
SinI	GGWCC	1 cut(s)	1807
SmaI	CCCGGG	1 cut(s)	3090
SmiMI	CAYNNNNRTG	1 cut(s)	1013
SmlI	CTYRAG	1 cut(s)	1302
SmoI	CTYRAG	1 cut(s)	1302
SseBI	AGGCCT	1 cut(s)	205
SspI	AATATT	3 cut(s)	938, 1212, 2782
SspMI	CTAG	5 cut(s)	1401, 1419, 1496, 2051, 3042
SstI	GAGCTC	1 cut(s)	1228
StuI	AGGCCT	1 cut(s)	205
StyI	CCWWGG	3 cut(s)	1810, 2489, 2809
TaiI	ACGT	5 cut(s)	63, 96, 1435, 1615, 1672
TatI	WGTACW	6 cut(s)	294, 1914, 2238, 2273, 2374, 2786
TauI	GCSGC	2 cut(s)	386, 3108
TfiI	GAWTC	8 cut(s)	100, 334, 467, 1316, 1543, 2501, 2576, 2909
TscAI	CASTG	6 cut(s)	242, 1711, 1766, 2248, 2439, 2986
TseFI	GTSAC	5 cut(s)	453, 2131, 2798, 2804, 3188
TseI	GCWGC	9 cut(s)	362, 386, 403, 821, 1295, 1463, 1886, 2837, 2984
Tsp45I	GTSAC	5 cut(s)	453, 2131, 2798, 2804, 3188
TspGWI	ACGGA	3 cut(s)	862, 2099, 2715
TspMI	CCCGGG	1 cut(s)	3088
TspRI	CASTG	6 cut(s)	242, 1711, 1766, 2248, 2439, 2986
Tth111I	GACNNNGTC	1 cut(s)	2137
Van91I	CCANNNNNTGG	2 cut(s)	2276, 2738
VpaK11BI	GGWCC	1 cut(s)	1807
XagI	CCTNNNNNAGG	1 cut(s)	2994
XapI	RAATTY	8 cut(s)	735, 957, 1065, 1112, 1156, 1939, 2524, 2887
XceI	RCATGY	3 cut(s)	244, 1054, 2900
XcmI	CCANNNNNNNNNTGG	2 cut(s)	686, 3182
XmaI	CCCGGG	1 cut(s)	3088
XmiI	GTMKAC	4 cut(s)	57, 1101, 1590, 2901
XspI	CTAG	5 cut(s)	1401, 1419, 1496, 2051, 3042
ZraI	GACGTC	1 cut(s)	61
Zsp2I	ATGCAT	3 cut(s)	376, 2005, 2637

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.