pycom15g20930

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003928
Physical Location & Seq
Forward (+)
15016240 .. 15019641
3402 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1620 bp
ATGGTTTGGCTGGATTTTACAGTCGGTTATCCAGTGGGCCCCACAGAGGATCTGAAGTATAATTTCATAGCTTTCCCATTCCAATTCAGGCGACCGTTCCATTACACTCCGCATTCAAAACCCAGCAAAAACCCCACCCGCTCTCTCTCTGTGACCATGACTGTCGCCACCGTAGCCACCACTCACACCAAGAAGCTGGTGAAGCTCAGCCGCTCGCTCTTCCGATGGGGATTCAACTCCTCCAAATGCAAAATGCAAGCGAAGATGGCGGTGGCTAGAATAAAGCTGCTGAGGAATAAGAGGCAGGTGGTGGTGAAGCAGATGAGGCGTGACATCGCCTTGCTCCTACAATCTGGTCAAGATGCCACTGCTCGAATCCGGGTTGAACATGTGATCAGAGAACAGAACGTTTTGGCTGCCAATGAATTCATTGAGCTCTTCTGTGAATTAGTAGTCTCTAGGCTTTCTATCATCGCAAAGCAAAGGGAGTGTCCACCTGATCTCAAGGAAGGGATTGCAAGTTTAATCTTTGCCTCCCCAAGGTGCTCTGAGATTCCTGAACTGATCACTCTAAGGAATATTTTTGAGAAAAAGTACGGTAAAGATTTTGTATCTGCGGCTGTTGATGTACGTCCCAGCTGTGGTGTGAATCGCATGCTGATTGACAAGCTCTCTGTTAGAACCCCTACGGGCGAAGTAAAGTTGAAACTCCTGAAGGAGATAGCCAAGGAATACCAGGTTGAATGGGATACATCGGAATCTGAACAGGAGCTTCTCAAGCCTCCAGAAGAGCTTATACAAGGACCACGCAATTTTGTTAGTGCTACCAGCATGCCTGTGCAGAGTGTGCAGCCTCAATCTGTTGAAACTAACAAGCCAGACACTAGGACTAGTGGTGGTGTAGAACGAAGAACAAGTGGTGGTGGAGAACGAAGAACAAGTGGTGGTGGAGAACGCAGAACAAGTAGTGGTGGTGAAAGAGGACATATGCAATTTGTAGACTCAGCATCAGCTGCCGAAGCAGCTGCAAAATCTGCCAGTGAGGCAATGGCTGCTGCACAAGTTGCTGCATATTTGGCCAACAATCAAGCAGCTCAATCATCTTCGAGTGTCAATACCGGATTCGGAACCCCTTCAGGAAATTCCACTGGCCGCTTTATGCCTGATTCTCCACCATTCGGGTCTCAGAATATGGTCAATAATGGATTTGGAACCCCTTCTGGCAATTCCACTAGCTTCTTTACGCCAGATTCTCCATCAGTTGGCTCTCAGAATAGCGATCACCAATCTAAAGCTCCTGGAGTTGATAGGTCTCACTCTTCAAGAGATGAGGAGGCGATGCACGGCCATGAAAATGAGAAGCAATTCATCTACAGGAGGTACAGCTACAATAACGCCCCATCAGTACATTCAGATATTAAGTTTGATGAATCGGACTGTGATGAAGAAGTTGAAATGGAGTCACCTCCCAGCGGCTTTCATCAAGCACCTGAGCGTCCTCCACCACAAGTACCTTCAGCTGCAAGGCAGGACTCGGTTCCTCGAGTTCATCCAAAGTTACCGGATTATGACGCACTTGCTGCTCGCTTTGACGCCCTCAAGTATCGGAAGTCATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

540

Amino Acids

59.54

Weight (kDa)

9.09

Isoelectric Point (pI)

56.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 84 - 248 1.5e-61 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 295
Acc36I ACCTGC 1 cut(s) 295
AccB7I CCANNNNNTGG 1 cut(s) 1262
AccBSI CCGCTC 2 cut(s) 141, 213
AccI GTMKAC 1 cut(s) 999
AciI CCGC 7 cut(s) 110, 139, 211, 269, 617, 1153, 1473
AclI AACGTT 1 cut(s) 408
AclWI GGATC 1 cut(s) 57
AcoI YGGCCR 3 cut(s) 1077, 1150, 1345
AcsI RAATTY 2 cut(s) 425, 1141
AcuI CTGAAG 4 cut(s) 74, 734, 1119, 1500
AcyI GRCGYC 1 cut(s) 1593
AfaI GTAC 5 cut(s) 596, 630, 1382, 1407, 1512
AfiI CCNNNNNNNGG 6 cut(s) 46, 540, 641, 1178, 1262, 1472
AflIII ACRYGT 1 cut(s) 388
AgsI TTSAA 8 cut(s) 117, 235, 386, 706, 743, 866, 1323, 1454
AhlI ACTAGT 1 cut(s) 890
AjnI CCWGG 2 cut(s) 735, 1297
Alw21I GWGCWC 2 cut(s) 438, 548
Alw26I GTCTC 3 cut(s) 460, 1188, 1317
AlwI GGATC 1 cut(s) 57
Ama87I CYCGRG 1 cut(s) 1542
AoxI GGCC 4 cut(s) 37, 1077, 1150, 1345
ApaI GGGCCC 1 cut(s) 41
ApoI RAATTY 2 cut(s) 425, 1141
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
Asp700I GAANNNNTTC 3 cut(s) 1132, 1216, 1364
AspS9I GGNCC 3 cut(s) 37, 38, 803
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 5 cut(s) 211, 325, 986, 1274, 1455
AvaI CYCGRG 1 cut(s) 1542
AvaII GGWCC 1 cut(s) 803
BaeGI GKGCMC 1 cut(s) 41
BalI TGGCCA 1 cut(s) 1079
BanII GRGCYC 2 cut(s) 41, 438
BarI GAAGNNNNNNTAC 2 cut(s) 780, 812
Bbv12I GWGCWC 2 cut(s) 438, 548
BbvCI CCTCAGC 1 cut(s) 290
BccI CCATC 4 cut(s) 219, 259, 1264, 1408
BceAI ACGGC 1 cut(s) 1360
BcgI CGANNNNNNTGC 2 cut(s) 1007, 1041
BciT130I CCWGG 2 cut(s) 737, 1299
BciVI GTATCC 1 cut(s) 742
BclI TGATCA 2 cut(s) 393, 564
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 3 cut(s) 460, 1188, 1317
BcuI ACTAGT 1 cut(s) 890
BfaI CTAG 6 cut(s) 276, 459, 885, 891, 1233, 1618
BfmI CTRYAG 1 cut(s) 1372
BfuAI ACCTGC 1 cut(s) 295
BfuI GTATCC 1 cut(s) 742
BglI GCCNNNNNGGC 1 cut(s) 1043
BlpI GCTNAGC 1 cut(s) 206
Bme1390I CCNGG 3 cut(s) 380, 737, 1299
Bme18I GGWCC 1 cut(s) 803
BmeT110I CYCGRG 1 cut(s) 1542
BmgT120I GGNCC 3 cut(s) 37, 38, 803
BmiI GGNNCC 5 cut(s) 39, 40, 1129, 1213, 1539
BmrFI CCNGG 3 cut(s) 380, 737, 1299
BmsI GCATC 3 cut(s) 352, 1016, 1329
BpmI CTGGAG 2 cut(s) 768, 1320
Bpu10I CCTNAGC 2 cut(s) 290, 1491
Bpu1102I GCTNAGC 1 cut(s) 206
BpuEI CTTGAG 3 cut(s) 488, 761, 1583
BpuMI CCSGG 1 cut(s) 380
BsaHI GRCGYC 1 cut(s) 1593
BsaI GGTCTC 2 cut(s) 1188, 1317
BsaJI CCNNGG 2 cut(s) 539, 726
BsaWI WCCGGW 2 cut(s) 1118, 1561
Bsc4I CCNNNNNNNGG 6 cut(s) 46, 540, 641, 1178, 1262, 1472
Bse1I ACTGG 3 cut(s) 32, 1038, 1153
Bse3DI GCAATG 1 cut(s) 1053
BseBI CCWGG 2 cut(s) 737, 1299
BseDI CCNNGG 2 cut(s) 539, 726
BseGI GGATG 2 cut(s) 1549, 1613
BseLI CCNNNNNNNGG 6 cut(s) 46, 540, 641, 1178, 1262, 1472
BseMI GCAATG 1 cut(s) 1053
BseMII CTCAG 7 cut(s) 220, 281, 540, 1017, 1199, 1283, 1482
BseNI ACTGG 3 cut(s) 32, 1038, 1153
BseRI GAGGAG 2 cut(s) 229, 1346
BseSI GKGCMC 1 cut(s) 41
BseYI CCCAGC 3 cut(s) 122, 635, 1469
BsgI GTGCAG 3 cut(s) 860, 869, 1041
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 4 cut(s) 39, 1079, 1152, 1347
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 2 cut(s) 438, 548
BsiHKCI CYCGRG 1 cut(s) 1542
BsiSI CCGG 3 cut(s) 379, 1119, 1562
BslFI GGGAC 1 cut(s) 618
BslI CCNNNNNNNGG 6 cut(s) 46, 540, 641, 1178, 1262, 1472
BsmAI GTCTC 3 cut(s) 460, 1188, 1317
BsmFI GGGAC 1 cut(s) 618
BsmI GAATGC 1 cut(s) 112
BsnI GGCC 4 cut(s) 39, 1079, 1152, 1347
Bso31I GGTCTC 2 cut(s) 1188, 1317
BsoBI CYCGRG 1 cut(s) 1542
Bsp120I GGGCCC 1 cut(s) 37
Bsp1286I GDGCHC 3 cut(s) 41, 438, 548
Bsp143I GATC 5 cut(s) 49, 393, 499, 564, 1279
Bsp1720I GCTNAGC 1 cut(s) 206
BspACI CCGC 7 cut(s) 110, 139, 211, 269, 617, 1153, 1473
BspANI GGCC 4 cut(s) 39, 1079, 1152, 1347
BspCNI CTCAG 7 cut(s) 219, 282, 541, 1016, 1198, 1282, 1483
BspLI GGNNCC 5 cut(s) 39, 40, 1129, 1213, 1539
BspMI ACCTGC 1 cut(s) 295
BspPI GGATC 1 cut(s) 57
BspQI GCTCTTC 3 cut(s) 224, 443, 783
BspTNI GGTCTC 2 cut(s) 1188, 1317
BsrBI CCGCTC 2 cut(s) 141, 213
BsrDI GCAATG 1 cut(s) 1053
BsrI ACTGG 3 cut(s) 32, 1038, 1153
BssECI CCNNGG 2 cut(s) 539, 726
BssMI GATC 5 cut(s) 49, 393, 499, 564, 1279
BssNI GRCGYC 1 cut(s) 1593
BssT1I CCWWGG 2 cut(s) 539, 726
Bst2UI CCWGG 2 cut(s) 737, 1299
Bst4CI ACNGT 6 cut(s) 22, 96, 163, 172, 599, 1439
Bst6I CTCTTC 4 cut(s) 224, 443, 783, 1324
BstACI GRCGYC 1 cut(s) 1593
BstAPI GCANNNNNTGC 6 cut(s) 847, 1013, 1034, 1052, 1064, 1580
BstC8I GCNNGC 5 cut(s) 215, 258, 656, 833, 1585
BstDEI CTNAG 8 cut(s) 206, 290, 549, 572, 1003, 1185, 1269, 1491
BstENI CCTNNNNNAGG 1 cut(s) 538
BstF5I GGATG 2 cut(s) 1549, 1613
BstKTI GATC 5 cut(s) 52, 396, 502, 567, 1282
BstMAI GTCTC 3 cut(s) 460, 1188, 1317
BstMBI GATC 5 cut(s) 49, 393, 499, 564, 1279
BstMCI CGRYCG 1 cut(s) 95
BstNI CCWGG 2 cut(s) 737, 1299
BstNSI RCATGY 3 cut(s) 392, 658, 835
BstSCI CCNGG 3 cut(s) 378, 735, 1297
BstSFI CTRYAG 1 cut(s) 1372
BstSLI GKGCMC 1 cut(s) 41
BstX2I RGATCY 1 cut(s) 49
BstXI CCANNNNNNTGG 1 cut(s) 196
BstYI RGATCY 1 cut(s) 49
BsuI GTATCC 1 cut(s) 742
BsuRI GGCC 4 cut(s) 39, 1079, 1152, 1347
BtgZI GCGATG 3 cut(s) 319, 457, 1352
BtsCI GGATG 2 cut(s) 1549, 1613
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 4 cut(s) 39, 366, 1045, 1146
BveI ACCTGC 1 cut(s) 295
Cac8I GCNNGC 5 cut(s) 215, 258, 656, 833, 1585
Cfr13I GGNCC 3 cut(s) 37, 38, 803
CseI GACGC 3 cut(s) 1484, 1580, 1601
CsiI ACCWGGT 1 cut(s) 735
Csp6I GTAC 5 cut(s) 595, 629, 1381, 1406, 1511
CviAII CATG 5 cut(s) 157, 389, 655, 832, 1349
CviQI GTAC 5 cut(s) 595, 629, 1381, 1406, 1511
DdeI CTNAG 8 cut(s) 206, 290, 549, 572, 1003, 1185, 1269, 1491
DpnI GATC 5 cut(s) 51, 395, 501, 566, 1281
DpnII GATC 5 cut(s) 49, 393, 499, 564, 1279
EaeI YGGCCR 3 cut(s) 1077, 1150, 1345
Eam1104I CTCTTC 4 cut(s) 224, 443, 783, 1324
EarI CTCTTC 4 cut(s) 224, 443, 783, 1324
Ecl136II GAGCTC 1 cut(s) 436
Eco130I CCWWGG 2 cut(s) 539, 726
Eco24I GRGCYC 2 cut(s) 41, 438
Eco31I GGTCTC 2 cut(s) 1188, 1317
Eco47I GGWCC 1 cut(s) 803
Eco53kI GAGCTC 1 cut(s) 436
Eco57I CTGAAG 4 cut(s) 74, 734, 1119, 1500
Eco88I CYCGRG 1 cut(s) 1542
EcoICRI GAGCTC 1 cut(s) 436
EcoNI CCTNNNNNAGG 1 cut(s) 538
EcoO109I RGGNCCY 1 cut(s) 38
EcoRI GAATTC 1 cut(s) 425
EcoRII CCWGG 2 cut(s) 735, 1297
EcoT14I CCWWGG 2 cut(s) 539, 726
EcoT38I GRGCYC 2 cut(s) 41, 438
ErhI CCWWGG 2 cut(s) 539, 726
FaeI CATG 5 cut(s) 160, 392, 658, 835, 1352
FaqI GGGAC 1 cut(s) 618
FatI CATG 5 cut(s) 156, 388, 654, 831, 1348
FauI CCCGC 1 cut(s) 146
FauNDI CATATG 1 cut(s) 987
FbaI TGATCA 2 cut(s) 393, 564
FblI GTMKAC 1 cut(s) 999
FokI GGATG 2 cut(s) 1536, 1600
FriOI GRGCYC 2 cut(s) 41, 438
FspBI CTAG 6 cut(s) 276, 459, 885, 891, 1233, 1618
GsaI CCCAGC 3 cut(s) 126, 639, 1473
GsuI CTGGAG 2 cut(s) 768, 1320
HaeIII GGCC 4 cut(s) 39, 1079, 1152, 1347
HapII CCGG 3 cut(s) 379, 1119, 1562
HgaI GACGC 3 cut(s) 1484, 1580, 1601
Hin1I GRCGYC 1 cut(s) 1593
Hin1II CATG 5 cut(s) 160, 392, 658, 835, 1352
HpaII CCGG 3 cut(s) 379, 1119, 1562
HphI GGTGA 5 cut(s) 211, 325, 986, 1274, 1455
Hpy166II GTNNAC 2 cut(s) 494, 1000
Hpy188III TCNNGA 6 cut(s) 359, 557, 712, 785, 1137, 1323
Hpy8I GTNNAC 2 cut(s) 494, 1000
HpyAV CCTTC 5 cut(s) 503, 709, 1143, 1227, 1524
HpyCH4III ACNGT 6 cut(s) 22, 96, 163, 172, 599, 1439
HpyCH4IV ACGT 2 cut(s) 408, 631
HpyF3I CTNAG 8 cut(s) 206, 290, 549, 572, 1003, 1185, 1269, 1491
HpySE526I ACGT 2 cut(s) 408, 631
Hsp92I GRCGYC 1 cut(s) 1593
Hsp92II CATG 5 cut(s) 160, 392, 658, 835, 1352
Ksp22I TGATCA 2 cut(s) 393, 564
Kzo9I GATC 5 cut(s) 49, 393, 499, 564, 1279
LguI GCTCTTC 3 cut(s) 224, 443, 783
LmnI GCTCC 3 cut(s) 348, 769, 1300
LweI GCATC 3 cut(s) 352, 1016, 1329
MabI ACCWGGT 1 cut(s) 735
MaeI CTAG 6 cut(s) 276, 459, 885, 891, 1233, 1618
MaeII ACGT 2 cut(s) 408, 631
MaeIII GTNAC 4 cut(s) 151, 329, 1461, 1557
MalI GATC 5 cut(s) 51, 395, 501, 566, 1281
MbiI CCGCTC 2 cut(s) 141, 213
MboI GATC 5 cut(s) 49, 393, 499, 564, 1279
MboII GAAGA 9 cut(s) 211, 274, 430, 800, 921, 945, 1095, 1311, 1457
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 3 cut(s) 41, 438, 548
MlsI TGGCCA 1 cut(s) 1079
MluCI AATT 9 cut(s) 61, 83, 425, 446, 811, 992, 1141, 1225, 1364
MluNI TGGCCA 1 cut(s) 1079
MlyI GAGTC 3 cut(s) 995, 1469, 1526
Mox20I TGGCCA 1 cut(s) 1079
MroXI GAANNNNTTC 3 cut(s) 1132, 1216, 1364
MscI TGGCCA 1 cut(s) 1079
MseI TTAA 2 cut(s) 524, 1419
MslI CAYNNNNRTG 3 cut(s) 836, 1347, 1353
Msp20I TGGCCA 1 cut(s) 1079
MspA1I CMGCKG 5 cut(s) 639, 1013, 1025, 1473, 1520
MspI CCGG 3 cut(s) 379, 1119, 1562
MspR9I CCNGG 3 cut(s) 380, 737, 1299
Mva1269I GAATGC 1 cut(s) 112
MvaI CCWGG 2 cut(s) 737, 1299
NciI CCSGG 1 cut(s) 380
NdeI CATATG 1 cut(s) 987
NdeII GATC 5 cut(s) 49, 393, 499, 564, 1279
NlaIII CATG 5 cut(s) 160, 392, 658, 835, 1352
NlaIV GGNNCC 5 cut(s) 39, 40, 1129, 1213, 1539
NmuCI GTSAC 3 cut(s) 151, 329, 1461
NspI RCATGY 3 cut(s) 392, 658, 835
PaeI GCATGC 2 cut(s) 658, 835
PaeR7I CTCGAG 1 cut(s) 1542
PaqCI CACCTGC 1 cut(s) 295
PciI ACATGT 1 cut(s) 388
PciSI GCTCTTC 3 cut(s) 224, 443, 783
PctI GAATGC 1 cut(s) 112
PdmI GAANNNNTTC 3 cut(s) 1132, 1216, 1364
PfeI GAWTC 9 cut(s) 231, 375, 553, 649, 758, 1122, 1166, 1250, 1430
PflMI CCANNNNNTGG 1 cut(s) 1262
PfoI TCCNGGA 1 cut(s) 1297
PleI GAGTC 3 cut(s) 995, 1468, 1526
PpsI GAGTC 3 cut(s) 995, 1468, 1526
PscI ACATGT 1 cut(s) 388
Psp124BI GAGCTC 1 cut(s) 438
Psp1406I AACGTT 1 cut(s) 408
Psp6I CCWGG 2 cut(s) 735, 1297
PspFI CCCAGC 3 cut(s) 122, 635, 1469
PspGI CCWGG 2 cut(s) 735, 1297
PspN4I GGNNCC 5 cut(s) 39, 40, 1129, 1213, 1539
PspOMI GGGCCC 1 cut(s) 37
PspPI GGNCC 3 cut(s) 37, 38, 803
PspXI VCTCGAGB 1 cut(s) 1542
PsuI RGATCY 1 cut(s) 49
PvuII CAGCTG 4 cut(s) 639, 1013, 1025, 1520
RsaI GTAC 5 cut(s) 596, 630, 1382, 1407, 1512
RsaNI GTAC 5 cut(s) 595, 629, 1381, 1406, 1511
RseI CAYNNNNRTG 3 cut(s) 836, 1347, 1353
SacI GAGCTC 1 cut(s) 438
SapI GCTCTTC 3 cut(s) 224, 443, 783
SaqAI TTAA 2 cut(s) 524, 1419
Sau3AI GATC 5 cut(s) 49, 393, 499, 564, 1279
Sau96I GGNCC 3 cut(s) 37, 38, 803
SchI GAGTC 3 cut(s) 995, 1469, 1526
ScrFI CCNGG 3 cut(s) 380, 737, 1299
SduI GDGCHC 3 cut(s) 41, 438, 548
SexAI ACCWGGT 1 cut(s) 735
SfaNI GCATC 3 cut(s) 352, 1016, 1329
SfcI CTRYAG 1 cut(s) 1372
Sfr274I CTCGAG 1 cut(s) 1542
SinI GGWCC 1 cut(s) 803
SlaI CTCGAG 1 cut(s) 1542
SmiMI CAYNNNNRTG 3 cut(s) 836, 1347, 1353
SmlI CTYRAG 4 cut(s) 503, 776, 1542, 1598
SmoI CTYRAG 4 cut(s) 503, 776, 1542, 1598
SpeI ACTAGT 1 cut(s) 890
SphI GCATGC 2 cut(s) 658, 835
Sse9I AATT 9 cut(s) 61, 83, 425, 446, 811, 992, 1141, 1225, 1364
SsiI CCGC 7 cut(s) 110, 139, 211, 269, 617, 1153, 1473
SspI AATATT 1 cut(s) 580
SspMI CTAG 6 cut(s) 276, 459, 885, 891, 1233, 1618
SstI GAGCTC 1 cut(s) 438
StyD4I CCNGG 3 cut(s) 378, 735, 1297
StyI CCWWGG 2 cut(s) 539, 726
TaaI ACNGT 6 cut(s) 22, 96, 163, 172, 599, 1439
TaiI ACGT 2 cut(s) 411, 634
TaqI TCGA 3 cut(s) 373, 1106, 1543
TasI AATT 9 cut(s) 61, 83, 425, 446, 811, 992, 1141, 1225, 1364
TatI WGTACW 1 cut(s) 1405
TauI GCSGC 4 cut(s) 213, 620, 1155, 1476
TfiI GAWTC 9 cut(s) 231, 375, 553, 649, 758, 1122, 1166, 1250, 1430
Tru1I TTAA 2 cut(s) 524, 1419
Tru9I TTAA 2 cut(s) 524, 1419
TscAI CASTG 4 cut(s) 39, 373, 1045, 1153
TseFI GTSAC 3 cut(s) 151, 329, 1461
Tsp45I GTSAC 3 cut(s) 151, 329, 1461
TspDTI ATGAA 9 cut(s) 55, 418, 438, 1357, 1365, 1443, 1458, 1469, 1538
TspRI CASTG 4 cut(s) 39, 373, 1045, 1153
Van91I CCANNNNNTGG 1 cut(s) 1262
VpaK11BI GGWCC 1 cut(s) 803
XagI CCTNNNNNAGG 1 cut(s) 538
XapI RAATTY 2 cut(s) 425, 1141
XceI RCATGY 3 cut(s) 392, 658, 835
XcmI CCANNNNNNNNNTGG 1 cut(s) 1045
XhoI CTCGAG 1 cut(s) 1542
XmiI GTMKAC 1 cut(s) 999
XmnI GAANNNNTTC 3 cut(s) 1132, 1216, 1364
XspI CTAG 6 cut(s) 276, 459, 885, 891, 1233, 1618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.