pycom16g03630

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001162
Physical Location & Seq
Forward (+)
2246228 .. 2248639
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1122 bp
ATGGACTTTTCTCGCTCCCTCAGACTCCAAACTCTCGAGTTTCCTCTCAGATTCCAAACCCCAATCGACCACCTCCACCAAAACCCCACATTCTCGGTCCCCGGAGGAGCTCGCGGCCGGATTGTTTCAGCCTTGAGAAGGCCACCCATTGAAGGTTTGAGCGAGGAGCTGAACTCGATCGCCAGGCGGAACCTCGACTTTGCCCGCACTCGCCGGCAGGTTCGATCCGCTTTCGTTGAAGTTCAGGAACAGCTCGACCATGTCCTGTTTAAGTTTCCAACTGGGATCAGAACAGAGGAGTGGTACGAAAGGAATTCGAGGGGGGTGGAGATTTTCTGCAAAAGCTGGATGCCGAAACAAGGTGTTCGAATTAAAGGTATTGCCAGGCGAATTGCTGCAGCCGGGTACGCTGTATATGCCATGGACTATCCGGGTTTTGGTCTTTCAGAAGGATTGCATGGTTACGTCCCAAGCTTTGATCAATTAGCCGATGATGTGATCGAACAGTACACCAAGATTAAAGAAAGACCGGAGCTGAAAGGGTTGCCCCACTTTATATTGGGGCAGTCCATGGGTGGTGCCGTTACTCTCAAAGTGCACCTAAAGGAACCATATGCATGGGATGGAGTAGTTCTTGTGGCACCAATGTGTAAAATTGCAGAAGACGTAACTCCTCCAGCGGCAGCTCTGAAGATATTAACTCTCATGTCAAAAGTTATGCCGAAAGCAAAGCTTGTCCCACAGAAAGATTTAGCGGAGCTGGCCTTCAGAGACCTTCGGAAACGGGAAATGGCTGTCTACAATGTGATATGCTACAACGACAATGTGCGGTTGAAGACTGCTGTGGAACTGCTACAAGCTACTAAGGATATTGAGATGCAACTCGAGAAGGTATCAGCGCCACTCCTAGTTCTTCATGGAGCTGCAGACAAGGTGACTGATCCTCGGGTGAGCCAGTTTCTTTATGAGAAGGCCTCTAGCAAGGATAAAACCCTAAAACTCTACCCGGAAGGTTATCATTCCATTCTCGAAGGAGAACCCGATGACAGAATTCTTACCGTCCTTGACGATATTGTCACTTGGCTCGATTCCCGCGTTCCCCTTCATAGCTTTTCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

374

Amino Acids

42.23

Weight (kDa)

8.75

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 123 - 347 1.2e-62 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 127 - 346 4.6e-11 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 132 - 350 7.4e-11 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1073
Acc36I ACCTGC 1 cut(s) 208
AccB1I GGYRCC 2 cut(s) 578, 640
AccI GTMKAC 1 cut(s) 798
AccII CGCG 2 cut(s) 114, 1095
AciI CCGC 8 cut(s) 114, 187, 205, 228, 680, 755, 829, 1093
AclWI GGATC 3 cut(s) 219, 293, 935
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 2 cut(s) 313, 1050
AcuI CTGAAG 2 cut(s) 710, 751
AfaI GTAC 3 cut(s) 305, 407, 509
AfiI CCNNNNNNNGG 4 cut(s) 138, 152, 359, 437
AgsI TTSAA 3 cut(s) 152, 239, 835
AjnI CCWGG 2 cut(s) 182, 383
AleI CACNNNNGTG 1 cut(s) 646
Alw21I GWGCWC 2 cut(s) 112, 600
Alw26I GTCTC 1 cut(s) 765
Alw44I GTGCAC 1 cut(s) 596
AlwI GGATC 3 cut(s) 219, 293, 935
Ama87I CYCGRG 3 cut(s) 35, 884, 945
AoxI GGCC 4 cut(s) 115, 140, 762, 972
ApaLI GTGCAC 1 cut(s) 596
ApeKI GCWGC 4 cut(s) 395, 398, 683, 923
ApoI RAATTY 2 cut(s) 313, 1050
AspLEI GCGC 1 cut(s) 901
AspS9I GGNCC 1 cut(s) 97
AsuC2I CCSGG 4 cut(s) 102, 403, 432, 1007
AsuHPI GGTGA 2 cut(s) 946, 961
AsuII TTCGAA 1 cut(s) 367
AvaI CYCGRG 3 cut(s) 35, 884, 945
AvaII GGWCC 1 cut(s) 97
BaeGI GKGCMC 1 cut(s) 600
BanI GGYRCC 2 cut(s) 578, 640
BanII GRGCYC 1 cut(s) 112
BbsI GAAGAC 2 cut(s) 669, 842
Bbv12I GWGCWC 2 cut(s) 112, 600
BbvI GCAGC 4 cut(s) 382, 410, 695, 910
BccI CCATC 1 cut(s) 617
BceAI ACGGC 1 cut(s) 566
BciT130I CCWGG 2 cut(s) 184, 385
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 4 cut(s) 102, 403, 432, 1007
BcoDI GTCTC 1 cut(s) 765
BfaI CTAG 2 cut(s) 908, 978
BfmI CTRYAG 2 cut(s) 396, 924
BfoI RGCGCY 1 cut(s) 902
BfuAI ACCTGC 1 cut(s) 208
BisI GCNGC 6 cut(s) 115, 396, 399, 681, 684, 924
BlsI GCNGC 6 cut(s) 116, 397, 400, 682, 685, 925
Bme1390I CCNGG 6 cut(s) 102, 184, 385, 403, 432, 1007
Bme18I GGWCC 1 cut(s) 97
BmeT110I CYCGRG 3 cut(s) 35, 884, 945
BmgT120I GGNCC 1 cut(s) 97
BmiI GGNNCC 5 cut(s) 99, 191, 580, 609, 642
BmrFI CCNGG 6 cut(s) 102, 184, 385, 403, 432, 1007
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 2 cut(s) 339, 867
BmuI ACTGGG 1 cut(s) 291
BpiI GAAGAC 2 cut(s) 669, 842
BplI GAGNNNNNCTC 4 cut(s) 158, 190, 959, 991
BpmI CTGGAG 1 cut(s) 660
Bpu14I TTCGAA 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 154
BpuMI CCSGG 4 cut(s) 102, 403, 432, 1007
BsaI GGTCTC 1 cut(s) 765
BsaJI CCNNGG 4 cut(s) 100, 420, 570, 944
BsaWI WCCGGW 1 cut(s) 529
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 152, 359, 437
Bse118I RCCGGY 1 cut(s) 213
Bse1I ACTGG 2 cut(s) 286, 955
BseBI CCWGG 2 cut(s) 184, 385
BseDI CCNNGG 4 cut(s) 100, 420, 570, 944
BseGI GGATG 2 cut(s) 354, 628
BseLI CCNNNNNNNGG 4 cut(s) 138, 152, 359, 437
BseMII CTCAG 2 cut(s) 34, 61
BseNI ACTGG 2 cut(s) 286, 955
BseRI GAGGAG 4 cut(s) 120, 179, 311, 663
BseSI GKGCMC 1 cut(s) 600
BseX3I CGGCCG 1 cut(s) 115
BseXI GCAGC 4 cut(s) 382, 410, 695, 910
Bsh1236I CGCG 2 cut(s) 114, 1095
Bsh1285I CGRYCG 2 cut(s) 118, 180
BshFI GGCC 4 cut(s) 117, 142, 764, 974
BshNI GGYRCC 2 cut(s) 578, 640
BsiEI CGRYCG 2 cut(s) 118, 180
BsiHKAI GWGCWC 2 cut(s) 112, 600
BsiHKCI CYCGRG 3 cut(s) 35, 884, 945
BsiSI CCGG 7 cut(s) 102, 118, 214, 402, 431, 530, 1007
BslFI GGGAC 3 cut(s) 83, 452, 722
BslI CCNNNNNNNGG 4 cut(s) 138, 152, 359, 437
BsmAI GTCTC 1 cut(s) 765
BsmFI GGGAC 3 cut(s) 83, 452, 722
BsnI GGCC 4 cut(s) 117, 142, 764, 974
Bso31I GGTCTC 1 cut(s) 765
BsoBI CYCGRG 3 cut(s) 35, 884, 945
Bsp119I TTCGAA 1 cut(s) 367
Bsp1286I GDGCHC 2 cut(s) 112, 600
Bsp143I GATC 6 cut(s) 177, 224, 285, 478, 498, 940
Bsp19I CCATGG 2 cut(s) 420, 570
BspACI CCGC 8 cut(s) 114, 187, 205, 228, 680, 755, 829, 1093
BspANI GGCC 4 cut(s) 117, 142, 764, 974
BspCNI CTCAG 2 cut(s) 33, 60
BspFNI CGCG 2 cut(s) 114, 1095
BspLI GGNNCC 5 cut(s) 99, 191, 580, 609, 642
BspMAI CTGCAG 2 cut(s) 400, 928
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 3 cut(s) 219, 293, 935
BspT104I TTCGAA 1 cut(s) 367
BspT107I GGYRCC 2 cut(s) 578, 640
BspTNI GGTCTC 1 cut(s) 765
BsrFI RCCGGY 1 cut(s) 213
BsrI ACTGG 2 cut(s) 286, 955
BssAI RCCGGY 1 cut(s) 213
BssECI CCNNGG 4 cut(s) 100, 420, 570, 944
BssMI GATC 6 cut(s) 177, 224, 285, 478, 498, 940
BssT1I CCWWGG 2 cut(s) 420, 570
Bst2UI CCWGG 2 cut(s) 184, 385
Bst4CI ACNGT 2 cut(s) 507, 1060
BstBI TTCGAA 1 cut(s) 367
BstC8I GCNNGC 4 cut(s) 112, 205, 215, 762
BstDEI CTNAG 3 cut(s) 20, 47, 864
BstDSI CCRYGG 2 cut(s) 420, 570
BstENI CCTNNNNNAGG 1 cut(s) 136
BstF5I GGATG 2 cut(s) 354, 628
BstFNI CGCG 2 cut(s) 114, 1095
BstH2I RGCGCY 1 cut(s) 902
BstHHI GCGC 1 cut(s) 901
BstKTI GATC 6 cut(s) 180, 227, 288, 481, 501, 943
BstMAI GTCTC 1 cut(s) 765
BstMBI GATC 6 cut(s) 177, 224, 285, 478, 498, 940
BstMCI CGRYCG 2 cut(s) 118, 180
BstMWI GCNNNNNNNGC 3 cut(s) 407, 416, 761
BstNI CCWGG 2 cut(s) 184, 385
BstSCI CCNGG 6 cut(s) 100, 182, 383, 401, 430, 1005
BstSFI CTRYAG 2 cut(s) 396, 924
BstSLI GKGCMC 1 cut(s) 600
BstUI CGCG 2 cut(s) 114, 1095
BstV1I GCAGC 4 cut(s) 382, 410, 695, 910
BstV2I GAAGAC 2 cut(s) 669, 842
BstXI CCANNNNNNTGG 1 cut(s) 618
BstZI CGGCCG 1 cut(s) 115
BsuRI GGCC 4 cut(s) 117, 142, 764, 974
BtgI CCRYGG 2 cut(s) 420, 570
BtsCI GGATG 2 cut(s) 354, 628
BveI ACCTGC 1 cut(s) 208
Cac8I GCNNGC 4 cut(s) 112, 205, 215, 762
CfoI GCGC 1 cut(s) 901
Cfr10I RCCGGY 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 97
Csp6I GTAC 3 cut(s) 304, 406, 508
CviAII CATG 7 cut(s) 260, 421, 458, 571, 618, 706, 917
CviQI GTAC 3 cut(s) 304, 406, 508
DdeI CTNAG 3 cut(s) 20, 47, 864
DpnI GATC 6 cut(s) 179, 226, 287, 480, 500, 942
DpnII GATC 6 cut(s) 177, 224, 285, 478, 498, 940
DrdI GACNNNNNNGTC 1 cut(s) 1073
DseDI GACNNNNNNGTC 1 cut(s) 1073
EaeI YGGCCR 1 cut(s) 115
EagI CGGCCG 1 cut(s) 115
EciI GGCGGA 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 110
EclXI CGGCCG 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 420, 570
Eco147I AGGCCT 1 cut(s) 974
Eco24I GRGCYC 1 cut(s) 112
Eco31I GGTCTC 1 cut(s) 765
Eco47I GGWCC 1 cut(s) 97
Eco52I CGGCCG 1 cut(s) 115
Eco53kI GAGCTC 1 cut(s) 110
Eco57I CTGAAG 2 cut(s) 710, 751
Eco88I CYCGRG 3 cut(s) 35, 884, 945
EcoICRI GAGCTC 1 cut(s) 110
EcoNI CCTNNNNNAGG 1 cut(s) 136
EcoRI GAATTC 2 cut(s) 313, 1050
EcoRII CCWGG 2 cut(s) 182, 383
EcoT14I CCWWGG 2 cut(s) 420, 570
EcoT22I ATGCAT 1 cut(s) 619
EcoT38I GRGCYC 1 cut(s) 112
ErhI CCWWGG 2 cut(s) 420, 570
FaeI CATG 7 cut(s) 263, 424, 461, 574, 621, 709, 920
FalI AAGNNNNNCTT 2 cut(s) 717, 749
FaqI GGGAC 3 cut(s) 83, 452, 722
FatI CATG 7 cut(s) 259, 420, 457, 570, 617, 705, 916
FauI CCCGC 2 cut(s) 212, 1100
FauNDI CATATG 1 cut(s) 613
FbaI TGATCA 1 cut(s) 478
FblI GTMKAC 1 cut(s) 798
Fnu4HI GCNGC 6 cut(s) 115, 396, 399, 681, 684, 924
FokI GGATG 2 cut(s) 361, 635
FriOI GRGCYC 1 cut(s) 112
Fsp4HI GCNGC 6 cut(s) 115, 396, 399, 681, 684, 924
FspBI CTAG 2 cut(s) 908, 978
GlaI GCGC 1 cut(s) 900
GluI GCNGC 6 cut(s) 115, 396, 399, 681, 684, 924
GsuI CTGGAG 1 cut(s) 660
HaeII RGCGCY 1 cut(s) 902
HaeIII GGCC 4 cut(s) 117, 142, 764, 974
HapII CCGG 7 cut(s) 102, 118, 214, 402, 431, 530, 1007
HhaI GCGC 1 cut(s) 901
Hin1II CATG 7 cut(s) 263, 424, 461, 574, 621, 709, 920
Hin6I GCGC 1 cut(s) 899
HinP1I GCGC 1 cut(s) 899
HindIII AAGCTT 2 cut(s) 472, 731
HinfI GANTC 3 cut(s) 24, 51, 1088
HpaII CCGG 7 cut(s) 102, 118, 214, 402, 431, 530, 1007
HphI GGTGA 2 cut(s) 946, 961
Hpy166II GTNNAC 3 cut(s) 510, 598, 799
Hpy188I TCNGA 8 cut(s) 23, 50, 290, 448, 690, 770, 780, 1121
Hpy188III TCNNGA 4 cut(s) 35, 245, 886, 1028
Hpy8I GTNNAC 3 cut(s) 510, 598, 799
HpyCH4III ACNGT 2 cut(s) 507, 1060
HpyCH4IV ACGT 2 cut(s) 465, 666
HpyCH4V TGCA 8 cut(s) 339, 398, 457, 598, 617, 659, 880, 926
HpyF10VI GCNNNNNNNGC 3 cut(s) 407, 416, 761
HpyF3I CTNAG 3 cut(s) 20, 47, 864
HpySE526I ACGT 2 cut(s) 465, 666
Hsp92II CATG 7 cut(s) 263, 424, 461, 574, 621, 709, 920
HspAI GCGC 1 cut(s) 899
KroI GCCGGC 1 cut(s) 213
KroNI GCCGGC 1 cut(s) 215
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 6 cut(s) 177, 224, 285, 478, 498, 940
LmnI GCTCC 6 cut(s) 20, 107, 166, 532, 757, 920
Lsp1109I GCAGC 4 cut(s) 382, 410, 695, 910
LweI GCATC 2 cut(s) 339, 867
MaeI CTAG 2 cut(s) 908, 978
MaeII ACGT 2 cut(s) 465, 666
MaeIII GTNAC 5 cut(s) 461, 583, 667, 934, 1075
MalI GATC 6 cut(s) 179, 226, 287, 480, 500, 942
MboI GATC 6 cut(s) 177, 224, 285, 478, 498, 940
MboII GAAGA 4 cut(s) 674, 703, 847, 905
MhlI GDGCHC 2 cut(s) 112, 600
MluCI AATT 6 cut(s) 313, 369, 390, 482, 654, 1050
MlyI GAGTC 1 cut(s) 18
MmeI TCCRAC 1 cut(s) 302
Mph1103I ATGCAT 1 cut(s) 619
MroNI GCCGGC 1 cut(s) 213
MseI TTAA 4 cut(s) 270, 372, 519, 698
MslI CAYNNNNRTG 2 cut(s) 616, 646
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 7 cut(s) 102, 118, 214, 402, 431, 530, 1007
MspR9I CCNGG 6 cut(s) 102, 184, 385, 403, 432, 1007
MvaI CCWGG 2 cut(s) 184, 385
MvnI CGCG 2 cut(s) 114, 1095
MwoI GCNNNNNNNGC 3 cut(s) 407, 416, 761
NaeI GCCGGC 1 cut(s) 215
NciI CCSGG 4 cut(s) 102, 403, 432, 1007
NcoI CCATGG 2 cut(s) 420, 570
NdeI CATATG 1 cut(s) 613
NdeII GATC 6 cut(s) 177, 224, 285, 478, 498, 940
NgoMIV GCCGGC 1 cut(s) 213
NlaIII CATG 7 cut(s) 263, 424, 461, 574, 621, 709, 920
NlaIV GGNNCC 5 cut(s) 99, 191, 580, 609, 642
NmuCI GTSAC 2 cut(s) 934, 1075
NsiI ATGCAT 1 cut(s) 619
NspV TTCGAA 1 cut(s) 367
OliI CACNNNNGTG 1 cut(s) 646
PaeR7I CTCGAG 2 cut(s) 35, 884
PceI AGGCCT 1 cut(s) 974
PcsI WCGNNNNNNNCGW 1 cut(s) 1092
PdiI GCCGGC 1 cut(s) 215
PfeI GAWTC 2 cut(s) 51, 1088
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 6 cut(s) 116, 397, 400, 682, 685, 925
Ple19I CGATCG 1 cut(s) 180
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
Psp124BI GAGCTC 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 182, 383
PspGI CCWGG 2 cut(s) 182, 383
PspN4I GGNNCC 5 cut(s) 99, 191, 580, 609, 642
PspPI GGNCC 1 cut(s) 97
PstI CTGCAG 2 cut(s) 400, 928
PsyI GACNNNGTC 1 cut(s) 260
PvuI CGATCG 1 cut(s) 180
RsaI GTAC 3 cut(s) 305, 407, 509
RsaNI GTAC 3 cut(s) 304, 406, 508
RseI CAYNNNNRTG 2 cut(s) 616, 646
SacI GAGCTC 1 cut(s) 112
SaqAI TTAA 4 cut(s) 270, 372, 519, 698
SatI GCNGC 6 cut(s) 115, 396, 399, 681, 684, 924
Sau3AI GATC 6 cut(s) 177, 224, 285, 478, 498, 940
Sau96I GGNCC 1 cut(s) 97
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 6 cut(s) 102, 184, 385, 403, 432, 1007
SduI GDGCHC 2 cut(s) 112, 600
SfaNI GCATC 2 cut(s) 339, 867
SfcI CTRYAG 2 cut(s) 396, 924
Sfr274I CTCGAG 2 cut(s) 35, 884
SfuI TTCGAA 1 cut(s) 367
SinI GGWCC 1 cut(s) 97
SlaI CTCGAG 2 cut(s) 35, 884
SmiMI CAYNNNNRTG 2 cut(s) 616, 646
SmlI CTYRAG 3 cut(s) 35, 133, 884
SmoI CTYRAG 3 cut(s) 35, 133, 884
Sse9I AATT 6 cut(s) 313, 369, 390, 482, 654, 1050
SseBI AGGCCT 1 cut(s) 974
SsiI CCGC 8 cut(s) 114, 187, 205, 228, 680, 755, 829, 1093
SspMI CTAG 2 cut(s) 908, 978
SstI GAGCTC 1 cut(s) 112
StuI AGGCCT 1 cut(s) 974
StyD4I CCNGG 6 cut(s) 100, 182, 383, 401, 430, 1005
StyI CCWWGG 2 cut(s) 420, 570
TaaI ACNGT 2 cut(s) 507, 1060
TaiI ACGT 2 cut(s) 468, 669
TaqII GACCGA 1 cut(s) 85
TasI AATT 6 cut(s) 313, 369, 390, 482, 654, 1050
TatI WGTACW 1 cut(s) 507
TauI GCSGC 2 cut(s) 117, 683
TfiI GAWTC 2 cut(s) 51, 1088
Tru1I TTAA 4 cut(s) 270, 372, 519, 698
Tru9I TTAA 4 cut(s) 270, 372, 519, 698
TseFI GTSAC 2 cut(s) 934, 1075
TseI GCWGC 4 cut(s) 395, 398, 683, 923
Tsp45I GTSAC 2 cut(s) 934, 1075
TspDTI ATGAA 2 cut(s) 905, 1094
Tth111I GACNNNGTC 1 cut(s) 260
VneI GTGCAC 1 cut(s) 596
VpaK11BI GGWCC 1 cut(s) 97
XagI CCTNNNNNAGG 1 cut(s) 136
XapI RAATTY 2 cut(s) 313, 1050
XhoI CTCGAG 2 cut(s) 35, 884
XmiI GTMKAC 1 cut(s) 798
XspI CTAG 2 cut(s) 908, 978
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.