pycom16g13970

epoxide hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000795
Physical Location & Seq
Forward (+)
10012863 .. 10013384
522 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 522 bp
ATGGAGTCGGAGGGAATAGAGCACCGAACGGTCCATGTGAACGGCATCAACATGCACGTCGCCGAGAAAGGGAATGGCCCACTCATCCTCTTCATCCACGGCTTTCCCGATCTCTGGTACTGCTGGCGCCACCAAATCCTCACCCTCTCCGCCCTCGGCTACCGCGCCGTCGCCCCCGACCTCCGAGGGTATGGCGACACCGACGCCCCCGTCTCCCCCTCCAGCTACACCTGCTTCCACATGGTTGGAGACCTCGTGGCGCTCCTGGACGCCATCGCACCCGATCAGGAGCAGGTGTTCGTGGTAGCCCACGACTGGGGCGCCATCATCGCTTGGTACCTCTGCCTGTTCCGGCCCGACCGGGTCAAAGCCTTGGTCAGCTTGAGCGTGCAGTTTCGGCCGCGAAACCCTCAGAGGAAGTTCATCGAAATGGTTCAAGCTGCTTATGGCGATGACTATTACATGTGCAGATTTCAGGTTTGGTACTTTTTATTTATTATTATTATTTTTGTAATTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

174

Amino Acids

19.93

Weight (kDa)

5.92

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 27 - 135 1.3e-22 alpha/beta hydrolase fold
Hydrolase_4 PF12146 28 - 145 1.5e-11 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 29 - 151 8.2e-17 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 239, 283
AasI GACNNNNNNGTC 1 cut(s) 209
Acc36I ACCTGC 2 cut(s) 239, 283
Acc65I GGTACC 1 cut(s) 336
AccB1I GGYRCC 3 cut(s) 126, 320, 336
AccII CGCG 2 cut(s) 165, 403
AciI CCGC 3 cut(s) 150, 163, 401
AcoI YGGCCR 1 cut(s) 398
AcyI GRCGYC 4 cut(s) 127, 204, 270, 321
AfaI GTAC 3 cut(s) 119, 338, 485
AfiI CCNNNNNNNGG 4 cut(s) 69, 114, 315, 316
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 1 cut(s) 437
AjiI CACGTC 1 cut(s) 58
AjnI CCWGG 1 cut(s) 264
AloI GAACNNNNNNTCC 2 cut(s) 281, 313
AluBI AGCT 3 cut(s) 225, 381, 440
AluI AGCT 3 cut(s) 225, 381, 440
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 2 cut(s) 217, 243
AoxI GGCC 3 cut(s) 76, 353, 398
ApeKI GCWGC 1 cut(s) 440
ArsI GACNNNNNNTTYG 2 cut(s) 360, 392
Asp700I GAANNNNTTC 1 cut(s) 432
Asp718I GGTACC 1 cut(s) 336
AspLEI GCGC 4 cut(s) 129, 167, 262, 323
AspS9I GGNCC 3 cut(s) 31, 77, 354
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 1 cut(s) 133
AvaII GGWCC 1 cut(s) 31
BanI GGYRCC 3 cut(s) 126, 320, 336
BauI CACGAG 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 1 cut(s) 427
BccI CCATC 2 cut(s) 281, 332
BceAI ACGGC 3 cut(s) 58, 115, 152
BciT130I CCWGG 1 cut(s) 266
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 2 cut(s) 217, 243
BfoI RGCGCY 3 cut(s) 130, 263, 324
BfuAI ACCTGC 2 cut(s) 239, 283
BisI GCNGC 2 cut(s) 401, 441
BlsI GCNGC 2 cut(s) 402, 442
Bme1390I CCNGG 2 cut(s) 266, 362
Bme18I GGWCC 1 cut(s) 31
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 3 cut(s) 31, 77, 354
BmiI GGNNCC 3 cut(s) 128, 322, 338
BmrFI CCNGG 2 cut(s) 266, 362
BmrI ACTGGG 1 cut(s) 325
BmsI GCATC 1 cut(s) 54
BmuI ACTGGG 1 cut(s) 325
BpmI CTGGAG 1 cut(s) 205
BpuEI CTTGAG 1 cut(s) 403
BpuMI CCSGG 1 cut(s) 362
BsaHI GRCGYC 4 cut(s) 127, 204, 270, 321
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 4 cut(s) 97, 154, 184, 372
BsaXI ACNNNNNCTCC 4 cut(s) 240, 270, 281, 311
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 114, 315, 316
Bse1I ACTGG 1 cut(s) 320
BseBI CCWGG 1 cut(s) 266
BseDI CCNNGG 4 cut(s) 97, 154, 184, 372
BseGI GGATG 2 cut(s) 84, 93
BseLI CCNNNNNNNGG 4 cut(s) 69, 114, 315, 316
BseMII CTCAG 1 cut(s) 425
BseNI ACTGG 1 cut(s) 320
BseX3I CGGCCG 1 cut(s) 398
BseXI GCAGC 1 cut(s) 427
BsgI GTGCAG 2 cut(s) 410, 487
Bsh1236I CGCG 2 cut(s) 165, 403
Bsh1285I CGRYCG 2 cut(s) 361, 401
BshFI GGCC 3 cut(s) 78, 355, 400
BshNI GGYRCC 3 cut(s) 126, 320, 336
BsiEI CGRYCG 2 cut(s) 361, 401
BsiHKAI GWGCWC 1 cut(s) 24
BsiSI CCGG 2 cut(s) 352, 361
BslI CCNNNNNNNGG 4 cut(s) 69, 114, 315, 316
BsmAI GTCTC 2 cut(s) 217, 243
BsmBI CGTCTC 1 cut(s) 217
BsnI GGCC 3 cut(s) 78, 355, 400
Bso31I GGTCTC 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 24
Bsp143I GATC 2 cut(s) 109, 283
BspACI CCGC 3 cut(s) 150, 163, 401
BspANI GGCC 3 cut(s) 78, 355, 400
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 2 cut(s) 165, 403
BspLI GGNNCC 3 cut(s) 128, 322, 338
BspMI ACCTGC 2 cut(s) 239, 283
BspT107I GGYRCC 3 cut(s) 126, 320, 336
BspTNI GGTCTC 1 cut(s) 243
BsrI ACTGG 1 cut(s) 320
BssECI CCNNGG 4 cut(s) 97, 154, 184, 372
BssMI GATC 2 cut(s) 109, 283
BssNI GRCGYC 4 cut(s) 127, 204, 270, 321
BssSI CACGAG 1 cut(s) 254
BssT1I CCWWGG 1 cut(s) 372
Bst2BI CACGAG 1 cut(s) 254
Bst2UI CCWGG 1 cut(s) 266
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 95
BstACI GRCGYC 4 cut(s) 127, 204, 270, 321
BstC8I GCNNGC 2 cut(s) 125, 389
BstDEI CTNAG 1 cut(s) 411
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 2 cut(s) 84, 93
BstFNI CGCG 2 cut(s) 165, 403
BstH2I RGCGCY 3 cut(s) 130, 263, 324
BstHHI GCGC 4 cut(s) 129, 167, 262, 323
BstKTI GATC 2 cut(s) 112, 286
BstMAI GTCTC 2 cut(s) 217, 243
BstMBI GATC 2 cut(s) 109, 283
BstMCI CGRYCG 2 cut(s) 361, 401
BstMWI GCNNNNNNNGC 3 cut(s) 231, 329, 397
BstNI CCWGG 1 cut(s) 266
BstNSI RCATGY 2 cut(s) 55, 466
BstSCI CCNGG 2 cut(s) 264, 360
BstUI CGCG 2 cut(s) 165, 403
BstV1I GCAGC 1 cut(s) 427
BstXI CCANNNNNNTGG 1 cut(s) 245
BstZI CGGCCG 1 cut(s) 398
BsuRI GGCC 3 cut(s) 78, 355, 400
BtgI CCRYGG 1 cut(s) 97
BtgZI GCGATG 3 cut(s) 259, 313, 465
BtrI CACGTC 1 cut(s) 58
BtsCI GGATG 2 cut(s) 84, 93
BveI ACCTGC 2 cut(s) 239, 283
Cac8I GCNNGC 2 cut(s) 125, 389
CfoI GCGC 4 cut(s) 129, 167, 262, 323
Cfr13I GGNCC 3 cut(s) 31, 77, 354
CseI GACGC 2 cut(s) 212, 278
Csp6I GTAC 3 cut(s) 118, 337, 484
CviAII CATG 4 cut(s) 35, 52, 241, 463
CviQI GTAC 3 cut(s) 118, 337, 484
DdeI CTNAG 1 cut(s) 411
DinI GGCGCC 2 cut(s) 128, 322
DpnI GATC 2 cut(s) 111, 285
DpnII GATC 2 cut(s) 109, 283
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 1 cut(s) 398
EagI CGGCCG 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
EciI GGCGGA 1 cut(s) 139
EclXI CGGCCG 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 31
Eco52I CGGCCG 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 372
EgeI GGCGCC 2 cut(s) 128, 322
EheI GGCGCC 2 cut(s) 128, 322
ErhI CCWWGG 1 cut(s) 372
Esp3I CGTCTC 1 cut(s) 217
FaeI CATG 4 cut(s) 38, 55, 244, 466
FaiI YATR 6 cut(s) 36, 53, 192, 242, 447, 464
FatI CATG 4 cut(s) 34, 51, 240, 462
Fnu4HI GCNGC 2 cut(s) 401, 441
FokI GGATG 2 cut(s) 71, 80
Fsp4HI GCNGC 2 cut(s) 401, 441
GlaI GCGC 4 cut(s) 128, 166, 261, 322
GluI GCNGC 2 cut(s) 401, 441
GsuI CTGGAG 1 cut(s) 205
HaeII RGCGCY 3 cut(s) 130, 263, 324
HaeIII GGCC 3 cut(s) 78, 355, 400
HapII CCGG 2 cut(s) 352, 361
HgaI GACGC 2 cut(s) 212, 278
HhaI GCGC 4 cut(s) 129, 167, 262, 323
Hin1I GRCGYC 4 cut(s) 127, 204, 270, 321
Hin1II CATG 4 cut(s) 38, 55, 244, 466
Hin6I GCGC 4 cut(s) 127, 165, 260, 321
HinP1I GCGC 4 cut(s) 127, 165, 260, 321
HinfI GANTC 1 cut(s) 5
HpaII CCGG 2 cut(s) 352, 361
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 3 cut(s) 10, 185, 414
Hpy188III TCNNGA 2 cut(s) 107, 287
Hpy8I GTNNAC 1 cut(s) 40
Hpy99I CGWCG 3 cut(s) 62, 173, 206
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 3 cut(s) 55, 391, 468
HpyF10VI GCNNNNNNNGC 3 cut(s) 231, 329, 397
HpyF3I CTNAG 1 cut(s) 411
HpySE526I ACGT 1 cut(s) 57
Hsp92I GRCGYC 4 cut(s) 127, 204, 270, 321
Hsp92II CATG 4 cut(s) 38, 55, 244, 466
HspAI GCGC 4 cut(s) 127, 165, 260, 321
KasI GGCGCC 2 cut(s) 126, 320
KpnI GGTACC 1 cut(s) 340
Kzo9I GATC 2 cut(s) 109, 283
LmnI GCTCC 2 cut(s) 267, 289
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 1 cut(s) 54
MaeII ACGT 1 cut(s) 57
MalI GATC 2 cut(s) 111, 285
MboI GATC 2 cut(s) 109, 283
MboII GAAGA 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 1 cut(s) 513
Mly113I GGCGCC 2 cut(s) 127, 321
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 432
MslI CAYNNNNRTG 2 cut(s) 50, 428
MspI CCGG 2 cut(s) 352, 361
MspR9I CCNGG 2 cut(s) 266, 362
MvaI CCWGG 1 cut(s) 266
MvnI CGCG 2 cut(s) 165, 403
MwoI GCNNNNNNNGC 3 cut(s) 231, 329, 397
NarI GGCGCC 2 cut(s) 127, 321
NciI CCSGG 1 cut(s) 362
NdeII GATC 2 cut(s) 109, 283
NlaIII CATG 4 cut(s) 38, 55, 244, 466
NlaIV GGNNCC 3 cut(s) 128, 322, 338
NmeAIII GCCGAG 2 cut(s) 88, 135
NspI RCATGY 2 cut(s) 55, 466
PaqCI CACCTGC 2 cut(s) 239, 283
PciI ACATGT 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 432
PflFI GACNNNGTC 1 cut(s) 362
PfoI TCCNGGA 1 cut(s) 264
PkrI GCNGC 2 cut(s) 402, 442
PleI GAGTC 1 cut(s) 13
PluTI GGCGCC 2 cut(s) 130, 324
PpsI GAGTC 1 cut(s) 13
PscI ACATGT 1 cut(s) 462
Psp6I CCWGG 1 cut(s) 264
PspGI CCWGG 1 cut(s) 264
PspN4I GGNNCC 3 cut(s) 128, 322, 338
PspPI GGNCC 3 cut(s) 31, 77, 354
PsyI GACNNNGTC 1 cut(s) 362
RsaI GTAC 3 cut(s) 119, 338, 485
RsaNI GTAC 3 cut(s) 118, 337, 484
RseI CAYNNNNRTG 2 cut(s) 50, 428
SatI GCNGC 2 cut(s) 401, 441
Sau3AI GATC 2 cut(s) 109, 283
Sau96I GGNCC 3 cut(s) 31, 77, 354
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 266, 362
SduI GDGCHC 1 cut(s) 24
SfaNI GCATC 1 cut(s) 54
SfoI GGCGCC 2 cut(s) 128, 322
SinI GGWCC 1 cut(s) 31
SmiMI CAYNNNNRTG 2 cut(s) 50, 428
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 1 cut(s) 513
SsiI CCGC 3 cut(s) 150, 163, 401
SspDI GGCGCC 2 cut(s) 126, 320
StyD4I CCNGG 2 cut(s) 264, 360
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 1 cut(s) 60
TaqI TCGA 1 cut(s) 426
TasI AATT 1 cut(s) 513
TauI GCSGC 1 cut(s) 403
TseI GCWGC 1 cut(s) 440
TspDTI ATGAA 2 cut(s) 82, 412
Tth111I GACNNNGTC 1 cut(s) 362
VpaK11BI GGWCC 1 cut(s) 31
XceI RCATGY 2 cut(s) 55, 466
XmnI GAANNNNTTC 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.