pycom16g19360

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001434
Physical Location & Seq
Reverse (-)
16344182 .. 16350202
6021 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2151 bp
ATGAGGGCAGGTTCTTCCGACGAGCCCAATTCTCCTTCCACCTCTGTGCCGCGCGTTCCTCAGCCCAATCCGTGCAAGCACAGTGTTAGCAATGTCCTTCGATTGTTAGCACGGAGAGAGGTTTCTCCGCAAACGAAACATTGGTCAAAGAAGTTATGGGGGGAACCTCCGAAGTCTAATTCCGATTCTGTTGGCCCAAAGTTTGAAACAGCTAGAGATGCTAGGCACAGCCTTGTCTCATGGGTGGAGGCAGAGTCACTCCAGCATTTGTCTTCCAAATATTGTCCGCTTGTGCCTCCTCCACGTTCAACTATTGCAGCAGCCTTCAGCCCCGATGGAACGACACTAGCTTCAACTCACGGAGACCATACTGTAAAGATTATTGATTGCCGAACTGGGATCTGCTTAAAGGTCTTGAGTGGTCACCGTAGGACACCGTGGGTGGTTAGATTTCATCCTGTGTATCCAGACATATTGGCTAGTGGAAGTCTGGATCATGAAGTTCGCTTGTGGGATGCAAACACGGCAGAATGTATAGGATCTCGTGATTTTTTGCGTCCCATTGCATCCATAGCTTTCCATGCCCAAGGTGAGCTGCTCGCAGTTGCGTCAGGTCACAAGTTGTACATATGGCACTACAATAGGAGAGGGGAGACATCCTCACCAAGCATAGTACTGAAGACACGACGTTCACTCCGGGCTGTGCATTTTCACCCACATGGAGCTCCTTTCCTTTTAACTGCTGAGGTCAATGACATGGACTCCTCAGATTCCTCGATGACACTTGCAACTTCTCCGGGTTATTTGCGCTATCCTCCACCTACCGTATATTTGGCAGATGGTCAATCTAGTACGATCGATCGTTCCAGTTTGGCAGATGGACTACCTCTTATGTCTTTACCATTTCTGATATGGCCTTCATTTGCTAGAGATAATGGGAGAATATCTATGCAGCGTAGTGACACGGACATTGGTTCAAGTAGTGTGCAACAGAGAGTTGATCCTTCTGCTTCAGTGCGGTTGCTAACATATTCAACTCCTTCAGGGCAGTATGAACTTCTGCTGTCCCCAATTGAACCTAGTAGCTCTTCTCCAGCACTAGAAGAGACAGAAACTAATACTTTCTTGAGTGAAATGGAAACTGAAGTTTCTCAACCTGCAATGGACAGTTTAGAGGCTTTGGAAGTGCAACCTGAAGGGAGGAGCAATCATATTTTCCCATTTGGTGACTCAACATATTGGGAACTTCCTTTTTTGCAAGGGTGGTTAATTGGTCAGACCCAAGCTAGCCAACGGAATATGCGGCTGGTAGGTGATGCTACACAAGATAATCCATCTGCACGTGGTGAGATGAATCCAGCCATAACTTCCTCGGTAATACCAACCAGTGTGAGCCAATCCAGGCTTACTGGAAGATCTAGTTCCCGGCATCGTACTTCACGAACTCATATGGTGTCGACGATTGGATCTAATGACGGTGCTGGATTCAATAGTTTTGCGCATGCTGAAAGTGAGCCTCAACCAGTTGCAAGTAGAATCCAATCGGAACTTGCCAACTCACTTGCTGCAGCAGCAGCTGCAGAGTTGCCTTGCACTGTAAAGCTCAGAATTTGGCCACACGATTTGAAAAATCCTTGTTCTCCCCTTGATGCAGAAAGATGTCGCTTAACCATTCCACATGCTGTACTTTGTAGTGAGATGGGTGCCCATTTTTCCCCTTGCGGAAGGTTTTTAGCTGCTTGTGTTGCATGTATGTTGCCCCATATGGAAGCTGATCCTGGATTGCAGAGCCAGGTCAACCATGATGCGACAGGGGCATCAACTTCCCCAACTCGACATCCAATTTCAGCACACCATGTCATTTCACTTCTTAAGAGTGTTGTCATTGATGGAGAGACAACAGTGCCTATTTACACCATTCTGGAGGTCTACAGAGTTTCTGATATGGAACTTGTGAGAGTTCTTCCTAGTGCAGAGGATGAGGTCAACGTAGCTTGCTTTCATCCTTCAGTTGGAGGTGGCCTTGTCTATGGAACCAAGGAAGGGAAGTTAAGGATCCTCCAATATGATAGTTCTCATTGCATGAATCAGACAACTGCTTTTCTTGATGAAAACATGCTTGAGGTCCCGACATATGCCTTGGAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

717

Amino Acids

78.13

Weight (kDa)

6.13

Isoelectric Point (pI)

55.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 106 - 211 5.4e-12 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 107 - 241 1.6e-19 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 108 - 252 2.3e-19 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 108 - 216 2.2e-11 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 108 - 215 2.4e-09 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 109 - 215 3.2e-06 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 112 - 242 7.2e-11 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 147 - 242 3e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1500
Acc36I ACCTGC 1 cut(s) 1165
AccB1I GGYRCC 1 cut(s) 1703
AccI GTMKAC 2 cut(s) 1457, 1929
AccII CGCG 2 cut(s) 52, 54
AciI CCGC 6 cut(s) 50, 128, 287, 1018, 1303, 1722
AclWI GGATC 8 cut(s) 407, 501, 547, 995, 1474, 1769, 2050, 2063
AcoI YGGCCR 1 cut(s) 1613
AcsI RAATTY 1 cut(s) 1608
AcuI CTGAAG 7 cut(s) 310, 698, 996, 1026, 1164, 1215, 1992
AcvI CACGTG 1 cut(s) 1343
AdeI CACNNNGTG 1 cut(s) 1346
AfaI GTAC 5 cut(s) 626, 675, 853, 1435, 1686
AfiI CCNNNNNNNGG 2 cut(s) 2012, 2043
AflII CTTAAG 1 cut(s) 1871
AgsI TTSAA 8 cut(s) 206, 309, 354, 978, 1035, 1076, 1489, 1627
AjnI CCWGG 3 cut(s) 1400, 1777, 1791
AleI CACNNNNGTG 1 cut(s) 44
Alw21I GWGCWC 1 cut(s) 727
Alw26I GTCTC 5 cut(s) 241, 357, 647, 1100, 1889
AlwI GGATC 8 cut(s) 407, 501, 547, 995, 1474, 1769, 2050, 2063
AlwNI CAGNNNCTG 1 cut(s) 1577
AoxI GGCC 4 cut(s) 193, 914, 1613, 2020
ApoI RAATTY 1 cut(s) 1608
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 3 cut(s) 54, 810, 1501
AspS9I GGNCC 2 cut(s) 194, 2125
AsuC2I CCSGG 3 cut(s) 698, 798, 1426
AsuHPI GGTGA 7 cut(s) 416, 602, 654, 704, 1238, 1325, 1358
AsuNHI GCTAGC 1 cut(s) 1286
AvaII GGWCC 1 cut(s) 2125
BaeGI GKGCMC 1 cut(s) 1708
BalI TGGCCA 1 cut(s) 1615
BamHI GGATCC 1 cut(s) 2055
BanI GGYRCC 1 cut(s) 1703
BanII GRGCYC 2 cut(s) 27, 727
BauI CACGAG 1 cut(s) 543
BbrPI CACGTG 1 cut(s) 1343
BbsI GAAGAC 2 cut(s) 264, 686
Bbv12I GWGCWC 1 cut(s) 727
BbvCI CCTCAGC 2 cut(s) 60, 744
BccI CCATC 6 cut(s) 329, 833, 872, 1342, 1693, 1883
BceAI ACGGC 1 cut(s) 540
BciT130I CCWGG 3 cut(s) 1402, 1779, 1793
BciVI GTATCC 1 cut(s) 474
BcnI CCSGG 3 cut(s) 698, 798, 1426
BcoDI GTCTC 5 cut(s) 241, 357, 647, 1100, 1889
BfmI CTRYAG 3 cut(s) 1566, 1578, 1930
BfrI CTTAAG 1 cut(s) 1871
BfuAI ACCTGC 1 cut(s) 1165
BfuI GTATCC 1 cut(s) 474
BglII AGATCT 1 cut(s) 1415
BmcAI AGTACT 1 cut(s) 675
Bme1390I CCNGG 6 cut(s) 698, 798, 1402, 1426, 1779, 1793
Bme18I GGWCC 1 cut(s) 2125
BmgT120I GGNCC 2 cut(s) 194, 2125
BmiI GGNNCC 5 cut(s) 165, 1705, 2035, 2057, 2127
BmrFI CCNGG 6 cut(s) 698, 798, 1402, 1426, 1779, 1793
BmrI ACTGGG 1 cut(s) 405
BmsI GCATC 8 cut(s) 208, 505, 575, 1306, 1438, 1639, 1795, 1826
BmtI GCTAGC 1 cut(s) 1290
BmuI ACTGGG 1 cut(s) 405
BpiI GAAGAC 2 cut(s) 264, 686
BplI GAGNNNNNCTC 2 cut(s) 644, 676
BpmI CTGGAG 3 cut(s) 245, 1077, 1943
Bpu10I CCTNAGC 2 cut(s) 60, 744
BpuEI CTTGAG 3 cut(s) 436, 1147, 2141
BpuMI CCSGG 3 cut(s) 698, 798, 1426
Bsa29I ATCGAT 1 cut(s) 858
BsaAI YACGTR 1 cut(s) 1343
BsaI GGTCTC 1 cut(s) 357
BsaJI CCNNGG 5 cut(s) 437, 586, 1371, 2037, 2139
BsaXI ACNNNNNCTCC 6 cut(s) 239, 269, 678, 708, 746, 776
Bsc4I CCNNNNNNNGG 2 cut(s) 2012, 2043
Bse1I ACTGG 5 cut(s) 400, 867, 1386, 1414, 1523
Bse3DI GCAATG 4 cut(s) 97, 561, 1167, 2077
BseBI CCWGG 3 cut(s) 1402, 1779, 1793
BseCI ATCGAT 1 cut(s) 858
BseDI CCNNGG 5 cut(s) 437, 586, 1371, 2037, 2139
BseGI GGATG 7 cut(s) 454, 520, 566, 656, 1837, 1984, 2002
BseLI CCNNNNNNNGG 2 cut(s) 2012, 2043
BseMI GCAATG 4 cut(s) 97, 561, 1167, 2077
BseMII CTCAG 4 cut(s) 74, 735, 780, 1618
BseNI ACTGG 5 cut(s) 400, 867, 1386, 1414, 1523
BseRI GAGGAG 3 cut(s) 288, 754, 1216
BseSI GKGCMC 1 cut(s) 1708
BsgI GTGCAG 2 cut(s) 1323, 1992
Bsh1236I CGCG 2 cut(s) 52, 54
Bsh1285I CGRYCG 2 cut(s) 858, 862
BshFI GGCC 4 cut(s) 195, 916, 1615, 2022
BshNI GGYRCC 1 cut(s) 1703
BshVI ATCGAT 1 cut(s) 858
BsiEI CGRYCG 2 cut(s) 858, 862
BsiHKAI GWGCWC 1 cut(s) 727
BsiSI CCGG 3 cut(s) 697, 797, 1426
BslFI GGGAC 3 cut(s) 543, 1051, 2111
BslI CCNNNNNNNGG 2 cut(s) 2012, 2043
BsmAI GTCTC 5 cut(s) 241, 357, 647, 1100, 1889
BsmFI GGGAC 3 cut(s) 543, 1051, 2111
BsmI GAATGC 1 cut(s) 2150
BsnI GGCC 4 cut(s) 195, 916, 1615, 2022
Bso31I GGTCTC 1 cut(s) 357
Bsp1286I GDGCHC 3 cut(s) 27, 727, 1708
Bsp1407I TGTACA 1 cut(s) 624
BspACI CCGC 6 cut(s) 50, 128, 287, 1018, 1303, 1722
BspANI GGCC 4 cut(s) 195, 916, 1615, 2022
BspCNI CTCAG 4 cut(s) 73, 736, 779, 1617
BspDI ATCGAT 1 cut(s) 858
BspFNI CGCG 2 cut(s) 52, 54
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 5 cut(s) 165, 1705, 2035, 2057, 2127
BspMAI CTGCAG 2 cut(s) 1570, 1582
BspMI ACCTGC 1 cut(s) 1165
BspOI GCTAGC 1 cut(s) 1290
BspPI GGATC 8 cut(s) 407, 501, 547, 995, 1474, 1769, 2050, 2063
BspQI GCTCTTC 1 cut(s) 1093
BspT107I GGYRCC 1 cut(s) 1703
BspTI CTTAAG 1 cut(s) 1871
BspTNI GGTCTC 1 cut(s) 357
BsrDI GCAATG 4 cut(s) 97, 561, 1167, 2077
BsrGI TGTACA 1 cut(s) 624
BsrI ACTGG 5 cut(s) 400, 867, 1386, 1414, 1523
BssECI CCNNGG 5 cut(s) 437, 586, 1371, 2037, 2139
BssSI CACGAG 1 cut(s) 543
BssT1I CCWWGG 3 cut(s) 586, 2037, 2139
Bst2BI CACGAG 1 cut(s) 543
Bst2UI CCWGG 3 cut(s) 1402, 1779, 1793
Bst4CI ACNGT 9 cut(s) 83, 373, 428, 438, 826, 1169, 1478, 1597, 1903
Bst6I CTCTTC 2 cut(s) 1093, 1098
BstAFI CTTAAG 1 cut(s) 1871
BstAPI GCANNNNNTGC 1 cut(s) 1577
BstAUI TGTACA 1 cut(s) 624
BstBAI YACGTR 1 cut(s) 1343
BstC8I GCNNGC 5 cut(s) 77, 600, 1288, 1503, 1996
BstDEI CTNAG 4 cut(s) 60, 744, 766, 1604
BstDSI CCRYGG 1 cut(s) 437
BstEII GGTNACC 1 cut(s) 422
BstF5I GGATG 7 cut(s) 454, 520, 566, 656, 1837, 1984, 2002
BstFNI CGCG 2 cut(s) 52, 54
BstHHI GCGC 3 cut(s) 54, 810, 1501
BstMAI GTCTC 5 cut(s) 241, 357, 647, 1100, 1889
BstMCI CGRYCG 2 cut(s) 858, 862
BstMWI GCNNNNNNNGC 9 cut(s) 218, 524, 572, 581, 1571, 1574, 1577, 1745, 1814
BstNI CCWGG 3 cut(s) 1402, 1779, 1793
BstNSI RCATGY 4 cut(s) 1505, 1682, 1752, 2119
BstPI GGTNACC 1 cut(s) 422
BstSCI CCNGG 6 cut(s) 696, 796, 1400, 1424, 1777, 1791
BstSFI CTRYAG 3 cut(s) 1566, 1578, 1930
BstSLI GKGCMC 1 cut(s) 1708
BstUI CGCG 2 cut(s) 52, 54
BstV2I GAAGAC 2 cut(s) 264, 686
BstX2I RGATCY 5 cut(s) 399, 539, 1415, 1466, 2055
BstYI RGATCY 5 cut(s) 399, 539, 1415, 1466, 2055
Bsu15I ATCGAT 1 cut(s) 858
BsuI GTATCC 1 cut(s) 474
BsuRI GGCC 4 cut(s) 195, 916, 1615, 2022
BsuTUI ATCGAT 1 cut(s) 858
BtgI CCRYGG 1 cut(s) 437
BtsCI GGATG 7 cut(s) 454, 520, 566, 656, 1837, 1984, 2002
BtsIMutI CAGTG 5 cut(s) 88, 1020, 1393, 1593, 1908
BveI ACCTGC 1 cut(s) 1165
Cac8I GCNNGC 5 cut(s) 77, 600, 1288, 1503, 1996
CaiI CAGNNNCTG 1 cut(s) 1577
CciI TCATGA 1 cut(s) 496
CfoI GCGC 3 cut(s) 54, 810, 1501
Cfr13I GGNCC 2 cut(s) 194, 2125
ClaI ATCGAT 1 cut(s) 858
CseI GACGC 2 cut(s) 545, 597
Csp6I GTAC 5 cut(s) 625, 674, 852, 1434, 1685
CspCI CAANNNNNGTGG 2 cut(s) 1605, 1640
CviQI GTAC 5 cut(s) 625, 674, 852, 1434, 1685
DdeI CTNAG 4 cut(s) 60, 744, 766, 1604
DraIII CACNNNGTG 1 cut(s) 1346
EaeI YGGCCR 1 cut(s) 1613
Eam1104I CTCTTC 2 cut(s) 1093, 1098
EarI CTCTTC 2 cut(s) 1093, 1098
Ecl136II GAGCTC 1 cut(s) 725
Eco130I CCWWGG 3 cut(s) 586, 2037, 2139
Eco24I GRGCYC 2 cut(s) 27, 727
Eco31I GGTCTC 1 cut(s) 357
Eco47I GGWCC 1 cut(s) 2125
Eco53kI GAGCTC 1 cut(s) 725
Eco57I CTGAAG 7 cut(s) 310, 698, 996, 1026, 1164, 1215, 1992
Eco72I CACGTG 1 cut(s) 1343
Eco91I GGTNACC 1 cut(s) 422
EcoICRI GAGCTC 1 cut(s) 725
EcoO109I RGGNCCY 1 cut(s) 2125
EcoO65I GGTNACC 1 cut(s) 422
EcoRII CCWGG 3 cut(s) 1400, 1777, 1791
EcoT14I CCWWGG 3 cut(s) 586, 2037, 2139
EcoT38I GRGCYC 2 cut(s) 27, 727
ErhI CCWWGG 3 cut(s) 586, 2037, 2139
FaqI GGGAC 3 cut(s) 543, 1051, 2111
FauNDI CATATG 4 cut(s) 629, 1449, 1764, 2134
FblI GTMKAC 2 cut(s) 1457, 1929
FokI GGATG 7 cut(s) 441, 527, 553, 643, 1824, 1989, 1991
FriOI GRGCYC 2 cut(s) 27, 727
FspI TGCGCA 1 cut(s) 1500
GlaI GCGC 3 cut(s) 53, 809, 1500
GsuI CTGGAG 3 cut(s) 245, 1077, 1943
HaeIII GGCC 4 cut(s) 195, 916, 1615, 2022
HapII CCGG 3 cut(s) 697, 797, 1426
HgaI GACGC 2 cut(s) 545, 597
HhaI GCGC 3 cut(s) 54, 810, 1501
Hin6I GCGC 3 cut(s) 52, 808, 1499
HinP1I GCGC 3 cut(s) 52, 808, 1499
HincII GTYRAC 3 cut(s) 1458, 1798, 1987
HindII GTYRAC 3 cut(s) 1458, 1798, 1987
HinfI GANTC 9 cut(s) 185, 254, 761, 770, 1229, 1354, 1485, 1536, 2086
HpaII CCGG 3 cut(s) 697, 797, 1426
HphI GGTGA 7 cut(s) 416, 602, 654, 704, 1238, 1325, 1358
Hpy166II GTNNAC 5 cut(s) 692, 1458, 1798, 1930, 1987
Hpy8I GTNNAC 5 cut(s) 692, 1458, 1798, 1930, 1987
Hpy99I CGWCG 3 cut(s) 23, 690, 1462
HpyCH4III ACNGT 9 cut(s) 83, 373, 428, 438, 826, 1169, 1478, 1597, 1903
HpyCH4IV ACGT 4 cut(s) 304, 688, 1342, 1989
HpyF10VI GCNNNNNNNGC 9 cut(s) 218, 524, 572, 581, 1571, 1574, 1577, 1745, 1814
HpyF3I CTNAG 4 cut(s) 60, 744, 766, 1604
HpySE526I ACGT 4 cut(s) 304, 688, 1342, 1989
HspAI GCGC 3 cut(s) 52, 808, 1499
LguI GCTCTTC 1 cut(s) 1093
LmnI GCTCC 3 cut(s) 722, 730, 1203
LweI GCATC 8 cut(s) 208, 505, 575, 1306, 1438, 1639, 1795, 1826
MaeII ACGT 4 cut(s) 304, 688, 1342, 1989
MaeIII GTNAC 5 cut(s) 255, 422, 614, 959, 1226
MboII GAAGA 7 cut(s) 6, 264, 691, 1080, 1115, 1425, 1955
MfeI CAATTG 1 cut(s) 1071
MflI RGATCY 5 cut(s) 399, 539, 1415, 1466, 2055
MhlI GDGCHC 3 cut(s) 27, 727, 1708
MlsI TGGCCA 1 cut(s) 1615
MluCI AATT 6 cut(s) 28, 178, 1071, 1269, 1608, 1842
MluNI TGGCCA 1 cut(s) 1615
MlyI GAGTC 3 cut(s) 263, 755, 1223
MmeI TCCRAC 2 cut(s) 42, 1993
Mox20I TGGCCA 1 cut(s) 1615
MscI TGGCCA 1 cut(s) 1615
MseI TTAA 6 cut(s) 407, 737, 1268, 1667, 1872, 2051
MslI CAYNNNNRTG 2 cut(s) 44, 717
Msp20I TGGCCA 1 cut(s) 1615
MspA1I CMGCKG 1 cut(s) 1577
MspCI CTTAAG 1 cut(s) 1871
MspI CCGG 3 cut(s) 697, 797, 1426
MspR9I CCNGG 6 cut(s) 698, 798, 1402, 1426, 1779, 1793
MunI CAATTG 1 cut(s) 1071
Mva1269I GAATGC 1 cut(s) 2150
MvaI CCWGG 3 cut(s) 1402, 1779, 1793
MvnI CGCG 2 cut(s) 52, 54
MwoI GCNNNNNNNGC 9 cut(s) 218, 524, 572, 581, 1571, 1574, 1577, 1745, 1814
NciI CCSGG 3 cut(s) 698, 798, 1426
NdeI CATATG 4 cut(s) 629, 1449, 1764, 2134
NheI GCTAGC 1 cut(s) 1286
NlaIV GGNNCC 5 cut(s) 165, 1705, 2035, 2057, 2127
NmuCI GTSAC 5 cut(s) 255, 422, 614, 959, 1226
NsbI TGCGCA 1 cut(s) 1500
NspI RCATGY 4 cut(s) 1505, 1682, 1752, 2119
OliI CACNNNNGTG 1 cut(s) 44
PaeI GCATGC 1 cut(s) 1505
PagI TCATGA 1 cut(s) 496
PciSI GCTCTTC 1 cut(s) 1093
PcsI WCGNNNNNNNCGW 1 cut(s) 1438
PctI GAATGC 1 cut(s) 2150
PfeI GAWTC 6 cut(s) 185, 770, 1354, 1485, 1536, 2086
PfoI TCCNGGA 1 cut(s) 1777
Ple19I CGATCG 2 cut(s) 858, 862
PleI GAGTC 3 cut(s) 262, 755, 1223
PmaCI CACGTG 1 cut(s) 1343
PmlI CACGTG 1 cut(s) 1343
PpsI GAGTC 3 cut(s) 262, 755, 1223
Ppu21I YACGTR 1 cut(s) 1343
PpuMI RGGWCCY 1 cut(s) 2125
Psp124BI GAGCTC 1 cut(s) 727
Psp5II RGGWCCY 1 cut(s) 2125
Psp6I CCWGG 3 cut(s) 1400, 1777, 1791
PspCI CACGTG 1 cut(s) 1343
PspEI GGTNACC 1 cut(s) 422
PspGI CCWGG 3 cut(s) 1400, 1777, 1791
PspN4I GGNNCC 5 cut(s) 165, 1705, 2035, 2057, 2127
PspPI GGNCC 2 cut(s) 194, 2125
PspPPI RGGWCCY 1 cut(s) 2125
PstI CTGCAG 2 cut(s) 1570, 1582
PstNI CAGNNNCTG 1 cut(s) 1577
PsuI RGATCY 5 cut(s) 399, 539, 1415, 1466, 2055
PvuI CGATCG 2 cut(s) 858, 862
PvuII CAGCTG 1 cut(s) 1577
RsaI GTAC 5 cut(s) 626, 675, 853, 1435, 1686
RsaNI GTAC 5 cut(s) 625, 674, 852, 1434, 1685
RseI CAYNNNNRTG 2 cut(s) 44, 717
SacI GAGCTC 1 cut(s) 727
SalI GTCGAC 1 cut(s) 1456
SapI GCTCTTC 1 cut(s) 1093
SaqAI TTAA 6 cut(s) 407, 737, 1268, 1667, 1872, 2051
Sau96I GGNCC 2 cut(s) 194, 2125
ScaI AGTACT 1 cut(s) 675
SchI GAGTC 3 cut(s) 263, 755, 1223
ScrFI CCNGG 6 cut(s) 698, 798, 1402, 1426, 1779, 1793
SduI GDGCHC 3 cut(s) 27, 727, 1708
SfaNI GCATC 8 cut(s) 208, 505, 575, 1306, 1438, 1639, 1795, 1826
SfcI CTRYAG 3 cut(s) 1566, 1578, 1930
SinI GGWCC 1 cut(s) 2125
SmiMI CAYNNNNRTG 2 cut(s) 44, 717
SmlI CTYRAG 4 cut(s) 415, 1126, 1871, 2120
SmoI CTYRAG 4 cut(s) 415, 1126, 1871, 2120
SphI GCATGC 1 cut(s) 1505
Sse9I AATT 6 cut(s) 28, 178, 1071, 1269, 1608, 1842
SsiI CCGC 6 cut(s) 50, 128, 287, 1018, 1303, 1722
SspI AATATT 1 cut(s) 281
SstI GAGCTC 1 cut(s) 727
StyD4I CCNGG 6 cut(s) 696, 796, 1400, 1424, 1777, 1791
StyI CCWWGG 3 cut(s) 586, 2037, 2139
TaaI ACNGT 9 cut(s) 83, 373, 428, 438, 826, 1169, 1478, 1597, 1903
TaiI ACGT 4 cut(s) 307, 691, 1345, 1992
TaqI TCGA 5 cut(s) 100, 776, 858, 1457, 1834
TasI AATT 6 cut(s) 28, 178, 1071, 1269, 1608, 1842
TatI WGTACW 3 cut(s) 624, 673, 1684
TauI GCSGC 2 cut(s) 52, 1306
TfiI GAWTC 6 cut(s) 185, 770, 1354, 1485, 1536, 2086
Tru1I TTAA 6 cut(s) 407, 737, 1268, 1667, 1872, 2051
Tru9I TTAA 6 cut(s) 407, 737, 1268, 1667, 1872, 2051
TscAI CASTG 5 cut(s) 88, 1020, 1393, 1600, 1908
TseFI GTSAC 5 cut(s) 255, 422, 614, 959, 1226
Tsp45I GTSAC 5 cut(s) 255, 422, 614, 959, 1226
TspDTI ATGAA 8 cut(s) 443, 513, 909, 1068, 1367, 1991, 2099, 2124
TspGWI ACGGA 5 cut(s) 60, 127, 375, 980, 1309
TspRI CASTG 5 cut(s) 88, 1020, 1393, 1600, 1908
Vha464I CTTAAG 1 cut(s) 1871
VpaK11BI GGWCC 1 cut(s) 2125
XapI RAATTY 1 cut(s) 1608
XceI RCATGY 4 cut(s) 1505, 1682, 1752, 2119
XcmI CCANNNNNNNNNTGG 1 cut(s) 909
XmiI GTMKAC 2 cut(s) 1457, 1929
ZrmI AGTACT 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.