Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0002242

Physical Location & Seq

Genomic Coordinates Reverse (-)

18805228 .. 18820206

Gene Structure 14979 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3513 bp

ATGGCTACTGACGAGACCAAGTTCAGCGTGCTCTGCAGCCTCTTCAACTGGATGCAGAAATCCAAAACCTCAACCCTAAAGCGCTCCAAATTCCGCAAATTTCTCGACAATTTCTGCAAACCCGCCGATTACTTCAGCTCCATTCGCCTCATTCTTCCCAGCCTCGACCGCGAGCGCGGCTCCTACGGCCTCAAGGAGTCCGTGCTTGCCACCTGCCTCATCGACGCCCTTGGCATCGCCAGAGACTCCGAGGACGCTCTCCGCCTAATTAATTGGCGCAAGGGTGGCGCCAAGACCGGCGCCAATGCCGGGAACTTCTCCCTCGTGGCGGCGGAGGTGTTGCAACGTAGGCAGGGTTTGAGTTCAGGTGGACTAACAATCAAGGAGTTAAATGACTTGCTTGATCGTTTAGCTTCGAGTGAAAACAGGGCAGAGAAGACTTCAGTTCTTTCGACCCTGATAAACAAGACAAATGCACAGGAAATGAAGTGGATTGTCATGATCATTCTTAAAGATCTGAAGCTGGGAACTAGTGAAAAGAGCATCTTTCACGAATTTCATCCGGATGCTGAAGATTTGTTTAATGTCACATGCGACTTGAAATTGGTCTGTGAAAAGCTCAGTGATAGAAATCAGCGCCATAAACGCCAGGACATTGAAGTTGGAAAAGCTGTGCGCCCACAACTAGCTATGAGAGTTGGTGATGCAAATATTGCATGGAAAAAGTTTCATGGTAAGGAAGTGGTTGTTGAGTGTAAATTTGATGGCGATCGCATCCAAATTCATAAGAACGGAAGTGAAGTACATTACTTCTCAAGGAATTTTCTTGAGCACTCTGAATACGCACATGCAATGTCAAACATTATCGTAGAAAATGTGCTGGCTGATAGGTGTATTCTTGATGGTGAGATGTTGGTCTGGGACACATCTTCTAATCGTTTTGCTGAGTTTGGTTCAAATCAAGAAATAGCCAAGGCAGCAAGGGACGGGATTGACAGTGATAGACAGTTGTGCTATGTTGCATTTGATATCCTCTATGTTGGAGATACTAGTGTGATTCACCAGAGCTTGAAGGAACGACATGAGCTTCTCCAGAAAGTTGTGAATCCATTGAAGGGTTGTTTAGAGATTTTAGTACCCAATCGTGGTCTTAACGCTCACCGTAATGAAGTCTCTTGTGATGGGAATGCAGGGGAACCTTGCTGGTCGCTTATTGCTAGTAATGTGGATGATGTTGAGAAGTTTTTCAAAGATACCATCGAAAATAGAGATGAGGGGATTGTTTTGAAGGACCTTGGTTCGAAATGGGAACCTAGTGATCGTAGTGGAAAATGGTTAAAATTAAAGCCTGACTACATTCGTGCAGGTTCTGATCTGGACGTTCTTATCATAGGAGGATACTATGGCTCTGGACGTCGTGGAGGGATGGTAGCTCAGTTCTTGGTGGGTCTTGCTGAGCGTCCGGTTTCAAATACATATCCTAGAAGGTTTGTATCCTTTTGCAGAGTGGGCACTGGACTTTCTGATGAAGAGATAGAAGCTGTTGCAACAAAATTAAAGCCGTATTTGAGGAAATATGAGTACCCAAAAAAGGCACCACCAAGTTTTTATCAAGTAACAGACAATTCTAAAGAGAGACCAGATGTTTGGGTTGAAAGCCCTGAGAAATCAATAATACTTTCTATAACGAGTGACATCAGAACTATAAAGTCTGAGGTTTTTGCTGCACCGTACAGCCTGAGATTTCCACGTATTGATCGTGTTAGATATGATAAACCTTGGCATGAATGCCTTGACGTGCAGTCATTTATAGAGCTTGTACAAGCAGGCAATGGGACCACACAAAGGGGGACGGATTATGGTGGTCTGCAGGATACTAAAAAGAGAAGCAAGAAGTCCCCTAGAAAGGAAGATAAAAGAAATCTGTCTATTGTCCCCTCTCATTTTGTTCAGACTGATGTTTCGGGCGTGAAGGAGGACACATTACTATTTTCAAATATGATGTTTTACTTTGTTAATGTGCCACCCACTCATTCTCTTGATTCCTTGCACAAGCTAGTTGTTGAGAATGGGGGTACTTTCTCTATGAATTTAAATAATGCAGTTACACACTGTGTTGCAGCTGATGGCAAAGGGATCAGGTATCAGGCAGCAAAGCGTCATGGAGATATCATTCATATCTCTTGGGTGTTGGATTGTTGTTCTCAAAAGAAACTCCTTCCATTACAGCCAAAGTACTATCTCTTCTTATCTGACTCTTCTAAGAAGAAGTTACAAGAAGAGATTGATGATTTTTCTGACTCGTACTACTGGGATCTTGACCTTGCGGAGATAAAGCAGCTTTTAAGCAACATTCATAGATCAGAGGACTCCAAAGTTGTTGACTATTACAAGAAAAAATACTGTCCGGTGGATAGATGGTCTCGCTTTCATGACTGCCGCATTTACTTTAAATCCTCAATTCATTCCTTGAAGCCGGATTGGGAAGTTGTCTTAGGACTCACACTGAGAAGGTTGAGGATTGAAGTTCTCAGGGGTGGTGGCATAGTCACTGACAGTCTTGTTCAGGCGACCCATTTGGTAGTCTTGTCCGTGCAGGGTCCCGATGGATTTGAAACTTTATTACGAAGTTTCACTGAAGGAGACAAACGATATCTGCATAGCAAAAGGTTGCATATTGTTGGATCTCAATGGTTGGAAGACTGCTTGGAAAGGGACCAAAGATTGCAAGAGGAAACATATAGCTTAAAACCCAATGGGTTGGAAGAATCCAACGTTGAAGAATGGAAACATGACATGGGTCTAGAAGAAGCACCCTGCGATGTGGATGCGGTGGAAAACCACGATTCTTCTTTTCCTAACCGGAAAGGAAAAGAGATTGAAGTAAAAGCGGCTCCAGAAAACTGTAAGATGTTGGAGTCACCCGAGAGGGGAAGCAAAAGGAAAAGGGGAAGACCTGCTCGCACAAGCTTGAACAAAGGAAAATCAACTGTCAACCAAGCTAGAAGAGCACGGGCGCGTATTGGAAGGAAGCCAGCTAAAATATCTGGCAACGAGTCTGAAGAAGGTGGCTCTCATGACAGTACATCACTTGGGGAAGAGGTTGATCTGGGAGAAAGAATTCACGGAACGGTAGGTCAGGAAAGTACAGACATCCAAGAAAATGAAGCAATGAAAGATTCTGAATCGTTACGAGGAGGCAAAGCAGTTGAGCAAGATGTCGCCGAGGACATTAGAATTGAAGAGCCTTACAAAATTGCTGAAATCAAAATGAAGGAGAAACAAAACGTCCAAGAGAGTAAAAAACGGGAGGAACTTGAAGCTACGGCTGATCCTGTTGAAGCCATGTTGTTTGACATGATTCCAAGTCTTGGTATGAAAAAAGCTGAAACTACATATACGAGTACTCGAGATGAGAAACCAGCACCGGATCCGATACCGAATGCAGGACCTTCAAAGAAAAAGAAAGTTAGCTACAAGGATATTGCTAGTGAGCTGCTAAAGGATTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005911 GO:0006139 GO:0006259 GO:0006266 GO:0006281 GO:0006289 GO:0006297 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009506 GO:0009628 GO:0009987 GO:0010165 GO:0010212 GO:0016874 GO:0016886 GO:0030054 GO:0032807 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048046 GO:0050896 GO:0051103 GO:0051716 GO:0055044 GO:0071704 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03450 map03450

Pfam Domains

Protein Families

BRCT_2 DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DNA_ligase_IV

Protein Analysis

1171

Amino Acids

132.51

Weight (kDa)

7.14

Isoelectric Point (pI)

44.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_ligase_A_N	PF04675	7 - 187	8.1e-38	DNA ligase N terminus
DNA_ligase_A_M	PF01068	227 - 449	2.9e-42	ATP dependent DNA ligase domain
RNA_ligase	PF09414	246 - 448	3.4e-07	RNA ligase
DNA_ligase_A_C	PF04679	473 - 592	1.9e-11	ATP dependent DNA ligase C terminal region
BRCT	PF00533	662 - 733	2.3e-07	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	663 - 744	3.6e-12	BRCT domain
PTCB-BRCT	PF12738	679 - 722	8.7e-08	twin BRCT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002242)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57160 AT5G57160
fragaria_vesca	FvH4_4g07160
malus_domestica	MD16G1247900.v1.1
prunus_persica	Prupe.1G074600_v2.0.a1 Prupe.1G074600_v2.0.a1
pyrus_communis	pycom12g16110 pycom12g16210 pycom16g20800
rosa_chinensis	RchiOBHm_Chr0c22g0500591 RchiOBHm_Chr4g0399311 RchiOBHm_Chr5g0052491 RchiOBHm_Chr7g0206661
rosa_laevigata	RLG00000009274 RLG00000012027 RLG00000013478 RLG00000013735 RLG00000034678
rosa_multiflora	Rmu_co8101330.1_g000001 Rmu_co8329359.1_g000001 Rmu_sc0000924.1_g000031 Rmu_sc0003135.1_g000008
rosa_roxburghii	Rroxscaffold_4G00283390 Rroxscaffold_4G00329320 Rroxscaffold_5G00344240
rosa_rugosa	Rorug04G0016500
rosa_samantha	Rh2AG218900 Rh2BG192100 Rh2BG230700 Rh2CG361900 Rh2DG225100 Rh3BG254200 Rh3DG357600 Rh4AG094200 Rh4AG102100 Rh4BG091600 Rh4CG102400 Rh4CG110300 Rh4DG086300 Rh7DG237100 Rh7DG335000 Rh7DG335200
rosa_wichuraiana	Rw4G007730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	221
AatII	GACGTC	1 cut(s)	1417
Acc36I	ACCTGC	3 cut(s)	221, 1355, 2965
AccB1I	GGYRCC	3 cut(s)	287, 299, 1594
AccB7I	CCANNNNNTGG	1 cut(s)	3371
AccII	CGCG	3 cut(s)	171, 177, 3019
AccIII	TCCGGA	1 cut(s)	562
AclI	AACGTT	1 cut(s)	2775
AclWI	GGATC	6 cut(s)	2144, 2322, 2692, 3326, 3425, 3438
AcsI	RAATTY	8 cut(s)	89, 98, 554, 758, 780, 820, 2089, 3120
AcuI	CTGAAG	6 cut(s)	118, 426, 539, 591, 2658, 3081
AcyI	GRCGYC	4 cut(s)	225, 288, 300, 1414
AdeI	CACNNNGTG	1 cut(s)	2114
AfeI	AGCGCT	1 cut(s)	83
AhdI	GACNNNNNGTC	1 cut(s)	1801
AhlI	ACTAGT	2 cut(s)	530, 1049
AjiI	CACGTC	1 cut(s)	1798
AjnI	CCWGG	1 cut(s)	648
Alw21I	GWGCWC	3 cut(s)	33, 834, 3013
Alw26I	GTCTC	6 cut(s)	8, 237, 1177, 1630, 2427, 2637
AlwI	GGATC	6 cut(s)	2144, 2322, 2692, 3326, 3425, 3438
AlwNI	CAGNNNCTG	1 cut(s)	1370
Ama87I	CYCGRG	2 cut(s)	2924, 3408
Aor13HI	TCCGGA	1 cut(s)	562
Aor51HI	AGCGCT	1 cut(s)	83
AoxI	GGCC	1 cut(s)	187
ApeKI	GCWGC	7 cut(s)	36, 977, 1724, 2120, 2150, 2338, 3496
ApoI	RAATTY	8 cut(s)	89, 98, 554, 758, 780, 820, 2089, 3120
ArsI	GACNNNNNNTTYG	4 cut(s)	1629, 1661, 1945, 1977
AseI	ATTAAT	1 cut(s)	270
AsiSI	GCGATCGC	1 cut(s)	772
Asp700I	GAANNNNTTC	2 cut(s)	1244, 3120
AspLEI	GCGC	8 cut(s)	84, 177, 279, 290, 302, 639, 678, 3019
AspS9I	GGNCC	5 cut(s)	1291, 1836, 2600, 2716, 3449
AsuC2I	CCSGG	1 cut(s)	310
AsuHPI	GGTGA	5 cut(s)	713, 917, 1052, 1151, 2913
AsuII	TTCGAA	1 cut(s)	1301
AvaI	CYCGRG	2 cut(s)	2924, 3408
AvaII	GGWCC	5 cut(s)	1291, 1836, 2600, 2716, 3449
BaeGI	GKGCMC	1 cut(s)	1514
BaeI	ACNNNNGTAYC	2 cut(s)	1038, 1071
BamHI	GGATCC	1 cut(s)	3430
BanI	GGYRCC	3 cut(s)	287, 299, 1594
BarI	GAAGNNNNNNTAC	2 cut(s)	2228, 2260
BauI	CACGAG	1 cut(s)	323
BbsI	GAAGAC	3 cut(s)	443, 2706, 2959
Bbv12I	GWGCWC	3 cut(s)	33, 834, 3013
BbvI	GCAGC	7 cut(s)	48, 989, 1711, 2132, 2162, 2350, 3483
BccI	CCATC	8 cut(s)	758, 896, 1175, 1265, 1420, 2120, 2412, 2600
BceAI	ACGGC	3 cut(s)	202, 1546, 3342
BcgI	CGANNNNNNTGC	4 cut(s)	85, 119, 2810, 2844
BciT130I	CCWGG	1 cut(s)	650
BciVI	GTATCC	3 cut(s)	1391, 1504, 1867
BclI	TGATCA	1 cut(s)	501
BcnI	CCSGG	1 cut(s)	310
BcoDI	GTCTC	6 cut(s)	8, 237, 1177, 1630, 2427, 2637
BcuI	ACTAGT	2 cut(s)	530, 1049
BfmI	CTRYAG	2 cut(s)	34, 1868
BfoI	RGCGCY	4 cut(s)	85, 291, 303, 640
BfuAI	ACCTGC	3 cut(s)	221, 1355, 2965
BfuI	GTATCC	3 cut(s)	1391, 1504, 1867
BglII	AGATCT	1 cut(s)	514
BlpI	GCTNAGC	1 cut(s)	1455
BmcAI	AGTACT	2 cut(s)	2237, 3406
Bme1390I	CCNGG	2 cut(s)	310, 650
Bme18I	GGWCC	5 cut(s)	1291, 1836, 2600, 2716, 3449
BmeRI	GACNNNNNGTC	1 cut(s)	1801
BmeT110I	CYCGRG	2 cut(s)	2924, 3408
BmgBI	CACGTC	1 cut(s)	1798
BmgT120I	GGNCC	5 cut(s)	1291, 1836, 2600, 2716, 3449
BmrFI	CCNGG	2 cut(s)	310, 650
BmrI	ACTGGG	1 cut(s)	2320
BmsI	GCATC	7 cut(s)	42, 243, 552, 556, 694, 783, 2818
BmuI	ACTGGG	1 cut(s)	2320
BoxI	GACNNNNGTC	1 cut(s)	2799
BpiI	GAAGAC	3 cut(s)	443, 2706, 2959
BplI	GAGNNNNNCTC	2 cut(s)	164, 196
BpmI	CTGGAG	2 cut(s)	1076, 2880
Bpu1102I	GCTNAGC	1 cut(s)	1455
Bpu14I	TTCGAA	1 cut(s)	1301
BpuEI	CTTGAG	3 cut(s)	176, 799, 848
BpuMI	CCSGG	1 cut(s)	310
BsaAI	YACGTR	1 cut(s)	1751
BsaBI	GATNNNNATC	2 cut(s)	630, 768
BsaHI	GRCGYC	4 cut(s)	225, 288, 300, 1414
BsaI	GGTCTC	3 cut(s)	8, 1630, 2427
BsaJI	CCNNGG	6 cut(s)	229, 249, 972, 1294, 1778, 3225
BsaWI	WCCGGW	5 cut(s)	562, 1462, 2407, 2862, 3427
BsaXI	ACNNNNNCTCC	4 cut(s)	122, 152, 1413, 1443
Bse118I	RCCGGY	1 cut(s)	296
Bse1I	ACTGG	3 cut(s)	53, 1519, 2315
Bse3DI	GCAATG	3 cut(s)	858, 1837, 3177
Bse8I	GATNNNNATC	2 cut(s)	630, 768
BseAI	TCCGGA	1 cut(s)	562
BseBI	CCWGG	1 cut(s)	650
BseDI	CCNNGG	6 cut(s)	229, 249, 972, 1294, 1778, 3225
BseGI	GGATG	8 cut(s)	57, 559, 571, 774, 1234, 1431, 2833, 3153
BseJI	GATNNNNATC	2 cut(s)	630, 768
BseMI	GCAATG	3 cut(s)	858, 1837, 3177
BseMII	CTCAG	9 cut(s)	634, 936, 1446, 1448, 1653, 1704, 1730, 2498, 2545
BseNI	ACTGG	3 cut(s)	53, 1519, 2315
BseRI	GAGGAG	1 cut(s)	3210
BseSI	GKGCMC	1 cut(s)	1514
BseXI	GCAGC	7 cut(s)	48, 989, 1711, 2132, 2162, 2350, 3483
BseYI	CCCAGC	2 cut(s)	158, 523
BsgI	GTGCAG	4 cut(s)	1383, 1710, 1820, 2615
Bsh1236I	CGCG	3 cut(s)	171, 177, 3019
Bsh1285I	CGRYCG	2 cut(s)	169, 772
BshFI	GGCC	1 cut(s)	189
BshNI	GGYRCC	3 cut(s)	287, 299, 1594
BsiEI	CGRYCG	2 cut(s)	169, 772
BsiHKAI	GWGCWC	3 cut(s)	33, 834, 3013
BsiHKCI	CYCGRG	2 cut(s)	2924, 3408
BsiSI	CCGG	8 cut(s)	297, 309, 563, 1463, 2408, 2477, 2863, 3428
BslFI	GGGAC	8 cut(s)	935, 998, 1849, 1864, 1882, 1919, 2586, 2729
BsmAI	GTCTC	6 cut(s)	8, 237, 1177, 1630, 2427, 2637
BsmFI	GGGAC	8 cut(s)	935, 998, 1849, 1864, 1882, 1919, 2586, 2729
BsmI	GAATGC	3 cut(s)	1192, 1793, 3448
BsnI	GGCC	1 cut(s)	189
Bso31I	GGTCTC	3 cut(s)	8, 1630, 2427
BsoBI	CYCGRG	2 cut(s)	2924, 3408
Bsp119I	TTCGAA	1 cut(s)	1301
Bsp1286I	GDGCHC	4 cut(s)	33, 834, 1514, 3013
Bsp13I	TCCGGA	1 cut(s)	562
Bsp1407I	TGTACA	1 cut(s)	1819
Bsp1720I	GCTNAGC	1 cut(s)	1455
BspANI	GGCC	1 cut(s)	189
BspCNI	CTCAG	9 cut(s)	633, 937, 1447, 1447, 1654, 1705, 1731, 2499, 2544
BspEI	TCCGGA	1 cut(s)	562
BspFNI	CGCG	3 cut(s)	171, 177, 3019
BspHI	TCATGA	3 cut(s)	498, 2431, 3076
BspMAI	CTGCAG	2 cut(s)	38, 1872
BspMI	ACCTGC	3 cut(s)	221, 1355, 2965
BspPI	GGATC	6 cut(s)	2144, 2322, 2692, 3326, 3425, 3438
BspQI	GCTCTTC	2 cut(s)	3001, 3237
BspT104I	TTCGAA	1 cut(s)	1301
BspT107I	GGYRCC	3 cut(s)	287, 299, 1594
BspTNI	GGTCTC	3 cut(s)	8, 1630, 2427
BsrDI	GCAATG	3 cut(s)	858, 1837, 3177
BsrFI	RCCGGY	1 cut(s)	296
BsrGI	TGTACA	1 cut(s)	1819
BsrI	ACTGG	3 cut(s)	53, 1519, 2315
BssAI	RCCGGY	1 cut(s)	296
BssECI	CCNNGG	6 cut(s)	229, 249, 972, 1294, 1778, 3225
BssNI	GRCGYC	4 cut(s)	225, 288, 300, 1414
BssSI	CACGAG	1 cut(s)	323
BssT1I	CCWWGG	4 cut(s)	229, 972, 1294, 1778
Bst2BI	CACGAG	1 cut(s)	323
Bst2UI	CCWGG	1 cut(s)	650
Bst6I	CTCTTC	8 cut(s)	47, 1524, 2249, 2263, 2274, 3001, 3093, 3237
BstACI	GRCGYC	4 cut(s)	225, 288, 300, 1414
BstAPI	GCANNNNNTGC	1 cut(s)	713
BstAUI	TGTACA	1 cut(s)	1819
BstBAI	YACGTR	1 cut(s)	1751
BstBI	TTCGAA	1 cut(s)	1301
BstC8I	GCNNGC	7 cut(s)	29, 173, 207, 882, 1828, 2962, 3036
BstF5I	GGATG	8 cut(s)	57, 559, 571, 774, 1234, 1431, 2833, 3153
BstFNI	CGCG	3 cut(s)	171, 177, 3019
BstH2I	RGCGCY	4 cut(s)	85, 291, 303, 640
BstHHI	GCGC	8 cut(s)	84, 177, 279, 290, 302, 639, 678, 3019
BstMAI	GTCTC	6 cut(s)	8, 237, 1177, 1630, 2427, 2637
BstMCI	CGRYCG	2 cut(s)	169, 772
BstNI	CCWGG	1 cut(s)	650
BstNSI	RCATGY	2 cut(s)	594, 851
BstPAI	GACNNNNGTC	1 cut(s)	2799
BstSCI	CCNGG	2 cut(s)	308, 648
BstSFI	CTRYAG	2 cut(s)	34, 1868
BstSLI	GKGCMC	1 cut(s)	1514
BstUI	CGCG	3 cut(s)	171, 177, 3019
BstV1I	GCAGC	7 cut(s)	48, 989, 1711, 2132, 2162, 2350, 3483
BstV2I	GAAGAC	3 cut(s)	443, 2706, 2959
BstX2I	RGATCY	4 cut(s)	514, 2314, 2684, 3430
BstXI	CCANNNNNNTGG	2 cut(s)	1647, 2761
BstYI	RGATCY	4 cut(s)	514, 2314, 2684, 3430
BsuI	GTATCC	3 cut(s)	1391, 1504, 1867
BsuRI	GGCC	1 cut(s)	189
BtgZI	GCGATG	2 cut(s)	220, 2835
BtrI	CACGTC	1 cut(s)	1798
BtsCI	GGATG	8 cut(s)	57, 559, 571, 774, 1234, 1431, 2833, 3153
BtsIMutI	CAGTG	7 cut(s)	628, 1003, 1512, 2110, 2504, 2550, 2634
BveI	ACCTGC	3 cut(s)	221, 1355, 2965
Cac8I	GCNNGC	7 cut(s)	29, 173, 207, 882, 1828, 2962, 3036
CaiI	CAGNNNCTG	1 cut(s)	1370
CciI	TCATGA	3 cut(s)	498, 2431, 3076
CfoI	GCGC	8 cut(s)	84, 177, 279, 290, 302, 639, 678, 3019
Cfr10I	RCCGGY	1 cut(s)	296
Cfr13I	GGNCC	5 cut(s)	1291, 1836, 2600, 2716, 3449
CseI	GACGC	4 cut(s)	233, 263, 1448, 2147
DinI	GGCGCC	2 cut(s)	289, 301
DraI	TTTAAA	2 cut(s)	2094, 2452
DraIII	CACNNNGTG	1 cut(s)	2114
DriI	GACNNNNNGTC	1 cut(s)	1801
Eam1104I	CTCTTC	8 cut(s)	47, 1524, 2249, 2263, 2274, 3001, 3093, 3237
Eam1105I	GACNNNNNGTC	1 cut(s)	1801
EarI	CTCTTC	8 cut(s)	47, 1524, 2249, 2263, 2274, 3001, 3093, 3237
EciI	GGCGGA	2 cut(s)	251, 347
Eco130I	CCWWGG	4 cut(s)	229, 972, 1294, 1778
Eco31I	GGTCTC	3 cut(s)	8, 1630, 2427
Eco32I	GATATC	3 cut(s)	1030, 2170, 2654
Eco47I	GGWCC	5 cut(s)	1291, 1836, 2600, 2716, 3449
Eco47III	AGCGCT	1 cut(s)	83
Eco57I	CTGAAG	6 cut(s)	118, 426, 539, 591, 2658, 3081
Eco88I	CYCGRG	2 cut(s)	2924, 3408
EcoO109I	RGGNCCY	3 cut(s)	1291, 2600, 3449
EcoRI	GAATTC	1 cut(s)	3120
EcoRII	CCWGG	1 cut(s)	648
EcoRV	GATATC	3 cut(s)	1030, 2170, 2654
EcoT14I	CCWWGG	4 cut(s)	229, 972, 1294, 1778
EgeI	GGCGCC	2 cut(s)	289, 301
EheI	GGCGCC	2 cut(s)	289, 301
ErhI	CCWWGG	4 cut(s)	229, 972, 1294, 1778
FalI	AAGNNNNNCTT	2 cut(s)	530, 562
FaqI	GGGAC	8 cut(s)	935, 998, 1849, 1864, 1882, 1919, 2586, 2729
FauI	CCCGC	1 cut(s)	130
FbaI	TGATCA	1 cut(s)	501
FokI	GGATG	8 cut(s)	64, 546, 578, 761, 1241, 1438, 2840, 3140
GlaI	GCGC	8 cut(s)	83, 176, 278, 289, 301, 638, 677, 3018
GsaI	CCCAGC	2 cut(s)	162, 527
GsuI	CTGGAG	2 cut(s)	1076, 2880
HaeII	RGCGCY	4 cut(s)	85, 291, 303, 640
HaeIII	GGCC	1 cut(s)	189
HapII	CCGG	8 cut(s)	297, 309, 563, 1463, 2408, 2477, 2863, 3428
HgaI	GACGC	4 cut(s)	233, 263, 1448, 2147
HhaI	GCGC	8 cut(s)	84, 177, 279, 290, 302, 639, 678, 3019
Hin1I	GRCGYC	4 cut(s)	225, 288, 300, 1414
Hin6I	GCGC	8 cut(s)	82, 175, 277, 288, 300, 637, 676, 3017
HinP1I	GCGC	8 cut(s)	82, 175, 277, 288, 300, 637, 676, 3017
HincII	GTYRAC	2 cut(s)	2383, 2995
HindII	GTYRAC	2 cut(s)	2383, 2995
HindIII	AAGCTT	1 cut(s)	2968
HpaII	CCGG	8 cut(s)	297, 309, 563, 1463, 2408, 2477, 2863, 3428
HphI	GGTGA	5 cut(s)	713, 917, 1052, 1151, 2913
Hpy166II	GTNNAC	3 cut(s)	371, 2383, 2995
Hpy8I	GTNNAC	3 cut(s)	371, 2383, 2995
Hpy99I	CGWCG	2 cut(s)	227, 1419
HpyCH4IV	ACGT	7 cut(s)	346, 1380, 1414, 1750, 1797, 2775, 3288
HpySE526I	ACGT	7 cut(s)	346, 1380, 1414, 1750, 1797, 2775, 3288
Hsp92I	GRCGYC	4 cut(s)	225, 288, 300, 1414
HspAI	GCGC	8 cut(s)	82, 175, 277, 288, 300, 637, 676, 3017
KasI	GGCGCC	2 cut(s)	287, 299
KflI	GGGWCCC	1 cut(s)	2600
Kpn2I	TCCGGA	1 cut(s)	562
Ksp22I	TGATCA	1 cut(s)	501
LguI	GCTCTTC	2 cut(s)	3001, 3237
LmnI	GCTCC	4 cut(s)	89, 143, 185, 2899
Lsp1109I	GCAGC	7 cut(s)	48, 989, 1711, 2132, 2162, 2350, 3483
LweI	GCATC	7 cut(s)	42, 243, 552, 556, 694, 783, 2818
MaeII	ACGT	7 cut(s)	346, 1380, 1414, 1750, 1797, 2775, 3288
MaeIII	GTNAC	8 cut(s)	586, 1615, 1691, 2104, 2271, 2548, 2919, 3189
MflI	RGATCY	4 cut(s)	514, 2314, 2684, 3430
MhlI	GDGCHC	4 cut(s)	33, 834, 1514, 3013
Mly113I	GGCGCC	2 cut(s)	288, 300
MlyI	GAGTC	8 cut(s)	206, 239, 2249, 2294, 2363, 2493, 2927, 3065
MmeI	TCCRAC	8 cut(s)	643, 1021, 2172, 2662, 2676, 2742, 2796, 2895
MroI	TCCGGA	1 cut(s)	562
MroXI	GAANNNNTTC	2 cut(s)	1244, 3120
MslI	CAYNNNNRTG	2 cut(s)	564, 1164
MspA1I	CMGCKG	1 cut(s)	2123
MspI	CCGG	8 cut(s)	297, 309, 563, 1463, 2408, 2477, 2863, 3428
MspR9I	CCNGG	2 cut(s)	310, 650
Mva1269I	GAATGC	3 cut(s)	1192, 1793, 3448
MvaI	CCWGG	1 cut(s)	650
MvnI	CGCG	3 cut(s)	171, 177, 3019
NarI	GGCGCC	2 cut(s)	288, 300
NciI	CCSGG	1 cut(s)	310
NmeAIII	GCCGAG	1 cut(s)	3250
NmuCI	GTSAC	4 cut(s)	586, 1691, 2548, 2919
NspI	RCATGY	2 cut(s)	594, 851
NspV	TTCGAA	1 cut(s)	1301
PaeR7I	CTCGAG	1 cut(s)	3408
PagI	TCATGA	3 cut(s)	498, 2431, 3076
PaqCI	CACCTGC	1 cut(s)	221
PciSI	GCTCTTC	2 cut(s)	3001, 3237
PcsI	WCGNNNNNNNCGW	1 cut(s)	1756
PctI	GAATGC	3 cut(s)	1192, 1793, 3448
PdmI	GAANNNNTTC	2 cut(s)	1244, 3120
PfeI	GAWTC	8 cut(s)	1057, 1105, 2042, 2768, 2846, 3179, 3185, 3361
PflMI	CCANNNNNTGG	1 cut(s)	3371
Ple19I	CGATCG	1 cut(s)	772
PleI	GAGTC	8 cut(s)	205, 239, 2249, 2294, 2363, 2493, 2926, 3064
PluTI	GGCGCC	2 cut(s)	291, 303
PpsI	GAGTC	8 cut(s)	205, 239, 2249, 2294, 2363, 2493, 2926, 3064
Ppu21I	YACGTR	1 cut(s)	1751
PpuMI	RGGWCCY	3 cut(s)	1291, 2600, 3449
PshAI	GACNNNNGTC	1 cut(s)	2799
PshBI	ATTAAT	1 cut(s)	270
Psp1406I	AACGTT	1 cut(s)	2775
Psp5II	RGGWCCY	3 cut(s)	1291, 2600, 3449
Psp6I	CCWGG	1 cut(s)	648
PspFI	CCCAGC	2 cut(s)	158, 523
PspGI	CCWGG	1 cut(s)	648
PspPI	GGNCC	5 cut(s)	1291, 1836, 2600, 2716, 3449
PspPPI	RGGWCCY	3 cut(s)	1291, 2600, 3449
PstI	CTGCAG	2 cut(s)	38, 1872
PstNI	CAGNNNCTG	1 cut(s)	1370
PsuI	RGATCY	4 cut(s)	514, 2314, 2684, 3430
PvuI	CGATCG	1 cut(s)	772
PvuII	CAGCTG	1 cut(s)	2123
RgaI	GCGATCGC	1 cut(s)	772
RseI	CAYNNNNRTG	2 cut(s)	564, 1164
SapI	GCTCTTC	2 cut(s)	3001, 3237
Sau96I	GGNCC	5 cut(s)	1291, 1836, 2600, 2716, 3449
ScaI	AGTACT	2 cut(s)	2237, 3406
SchI	GAGTC	8 cut(s)	206, 239, 2249, 2294, 2363, 2493, 2927, 3065
ScrFI	CCNGG	2 cut(s)	310, 650
SduI	GDGCHC	4 cut(s)	33, 834, 1514, 3013
SfaAI	GCGATCGC	1 cut(s)	772
SfaNI	GCATC	7 cut(s)	42, 243, 552, 556, 694, 783, 2818
SfcI	CTRYAG	2 cut(s)	34, 1868
SfoI	GGCGCC	2 cut(s)	289, 301
Sfr274I	CTCGAG	1 cut(s)	3408
SfuI	TTCGAA	1 cut(s)	1301
SgfI	GCGATCGC	1 cut(s)	772
SinI	GGWCC	5 cut(s)	1291, 1836, 2600, 2716, 3449
SlaI	CTCGAG	1 cut(s)	3408
SmiI	ATTTAAAT	1 cut(s)	2094
SmiMI	CAYNNNNRTG	2 cut(s)	564, 1164
SmlI	CTYRAG	4 cut(s)	191, 814, 827, 3408
SmoI	CTYRAG	4 cut(s)	191, 814, 827, 3408
SpeI	ACTAGT	2 cut(s)	530, 1049
SspDI	GGCGCC	2 cut(s)	287, 299
SspI	AATATT	1 cut(s)	712
StyD4I	CCNGG	2 cut(s)	308, 648
StyI	CCWWGG	4 cut(s)	229, 972, 1294, 1778
SwaI	ATTTAAAT	1 cut(s)	2094
TaiI	ACGT	7 cut(s)	349, 1383, 1417, 1753, 1800, 2778, 3291
TaqI	TCGA	8 cut(s)	105, 165, 222, 416, 452, 1260, 1301, 3409
TatI	WGTACW	6 cut(s)	802, 1819, 2235, 3083, 3146, 3404
TauI	GCSGC	4 cut(s)	180, 332, 2442, 2894
TfiI	GAWTC	8 cut(s)	1057, 1105, 2042, 2768, 2846, 3179, 3185, 3361
TscAI	CASTG	7 cut(s)	628, 1003, 1519, 2117, 2511, 2557, 2641
TseFI	GTSAC	4 cut(s)	586, 1691, 2548, 2919
TseI	GCWGC	7 cut(s)	36, 977, 1724, 2120, 2150, 2338, 3496
Tsp45I	GTSAC	4 cut(s)	586, 1691, 2548, 2919
TspGWI	ACGGA	5 cut(s)	190, 807, 1868, 2581, 3141
TspRI	CASTG	7 cut(s)	628, 1003, 1519, 2117, 2511, 2557, 2641
Van91I	CCANNNNNTGG	1 cut(s)	3371
VpaK11BI	GGWCC	5 cut(s)	1291, 1836, 2600, 2716, 3449
VspI	ATTAAT	1 cut(s)	270
XapI	RAATTY	8 cut(s)	89, 98, 554, 758, 780, 820, 2089, 3120
XbaI	TCTAGA	1 cut(s)	2803
XceI	RCATGY	2 cut(s)	594, 851
XhoI	CTCGAG	1 cut(s)	3408
XmnI	GAANNNNTTC	2 cut(s)	1244, 3120
ZraI	GACGTC	1 cut(s)	1415
ZrmI	AGTACT	2 cut(s)	2237, 3406

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.