pycom17g01880

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002827
Physical Location & Seq
Forward (+)
1190306 .. 1192385
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
ATGAACTTATGTACGGTACACACTGACCCTTTAAGACACGAGATGGGAGGCCAGAACTTACCACCCTGTTCTTTTCCGGCAATTTACAACTTCGGGGACTCAAATTCCGACACCGGCGGCATATCCGCTGCATTTGAGCCAATCAAACCTCCTAACGGCGAAGCATTCTTCCATAAGCCGGCCGGGAGGGCCGCCGATGGCCGCCTTGTCATAGACTTCATAGCTGCCGTACTTGAGTGCATATTTGAACTCACTTGGAACAAATTGCACGGTGCGAATTTCGCCACCGGCGGATCCACCGTCAGAAGGCCGAACGAAACCATCTTTGAGAACGGCATTAGTCCTTTCTCCCTCGACATGCAGACGGCGCAATTCCTGCAGTTCAAGGCCCGCACCGCCGATCTCTACCGCCAAGCCAAGAACCCTTCTCAGAGAAGCGCTCTGCCGAATCCTCAAGATTTCGCAAAGGCTCTCTACACGTTTGATATCGGGCAAAATGATCTATCCGCGGGTTTTCGGAAGCTAAGTTTCGACCAGCTACGTGCACAGCTGCCGGACATTGTCAACCAGTTAGCCATAGCAGTCCATCGGATATATGAAGAAGGAGGGAGGACATTTTGGATACACAACACAGGTCCTGTTGGTTGCTTGCCTATACAGTTATTCTACAATCTGAATCCGCCGCGCGGTTTTCTCGACGACCACGGCTGCGTCAAGGCCCAAAACGACATCGCCGTAGAGTTCAACAGGCAGCTAAGGGACAGAGTCACCAAACTCAGAGCCGAGCTCCCACAAGCTGCGATCACATATGTCGATGTCTTTGCCGCCAAGTATGGACTCATCGGCACGGCAAACACCGAAGGTACCCAATTTCTTCAACTTCATTACCACAAATTATCTTGTTCGATACCGATCAACGTTACAATTTTGTTTACAGGATTTGCCGAACCAATGAAGGTTTGCTGCGGGTATCATGTGAAATACGACCATGTCTGGTGCGGGAATAAAGGAAGTGCGAGCGGAAGGGAAGTGTACGGTGCTTCATGTCAAAACCCGTCGGCTTTTATCAGCTGGGATGGAATTCACTACACTCAGGGTGCGAACCAGTGGGTTGCTAATCGTATACTCAATGGCTCGCCTTCGTCAATCCCGGCAATTCCGATTACTAAGGCTTGTCATAGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

396

Amino Acids

43.65

Weight (kDa)

7.99

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 375 4.7e-38 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 863
AccBSI CCGCTC 1 cut(s) 1020
AccI GTMKAC 1 cut(s) 1123
AccII CGCG 3 cut(s) 509, 685, 687
AclI AACGTT 1 cut(s) 918
AclWI GGATC 2 cut(s) 288, 301
AcoI YGGCCR 2 cut(s) 180, 199
AcsI RAATTY 3 cut(s) 103, 277, 1080
AfaI GTAC 5 cut(s) 13, 18, 231, 865, 1034
AfeI AGCGCT 1 cut(s) 439
AfiI CCNNNNNNNGG 4 cut(s) 155, 178, 306, 686
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 4 cut(s) 248, 385, 745, 878
AluBI AGCT 8 cut(s) 224, 523, 538, 550, 754, 787, 797, 1071
AluI AGCT 8 cut(s) 224, 523, 538, 550, 754, 787, 797, 1071
Alw21I GWGCWC 2 cut(s) 547, 789
Alw44I GTGCAC 1 cut(s) 543
AlwI GGATC 2 cut(s) 288, 301
AlwNI CAGNNNCTG 1 cut(s) 638
Aor51HI AGCGCT 1 cut(s) 439
AoxI GGCC 7 cut(s) 49, 180, 189, 199, 308, 387, 717
ApaLI GTGCAC 1 cut(s) 543
ApeKI GCWGC 7 cut(s) 128, 224, 550, 708, 751, 797, 963
ApoI RAATTY 3 cut(s) 103, 277, 1080
Asp718I GGTACC 1 cut(s) 863
AspLEI GCGC 3 cut(s) 370, 440, 687
AspS9I GGNCC 4 cut(s) 189, 388, 635, 718
AsuC2I CCSGG 2 cut(s) 184, 1151
AsuHPI GGTGA 1 cut(s) 760
AvaII GGWCC 1 cut(s) 635
BaeGI GKGCMC 1 cut(s) 547
BamHI GGATCC 1 cut(s) 293
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 1 cut(s) 789
BauI CACGAG 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 547, 789
BbvI GCAGC 7 cut(s) 115, 211, 537, 695, 763, 784, 950
BccI CCATC 5 cut(s) 37, 191, 329, 594, 1070
BceAI ACGGC 7 cut(s) 172, 212, 349, 381, 719, 721, 864
BcgI CGANNNNNNTGC 2 cut(s) 803, 837
BciVI GTATCC 1 cut(s) 615
BcnI CCSGG 2 cut(s) 184, 1151
BfmI CTRYAG 1 cut(s) 377
BfoI RGCGCY 1 cut(s) 441
BfuI GTATCC 1 cut(s) 615
BglI GCCNNNNNGGC 1 cut(s) 188
Bme1390I CCNGG 2 cut(s) 184, 1151
Bme18I GGWCC 1 cut(s) 635
BmgT120I GGNCC 4 cut(s) 189, 388, 635, 718
BmiI GGNNCC 2 cut(s) 295, 865
BmrFI CCNGG 2 cut(s) 184, 1151
BplI GAGNNNNNCTC 4 cut(s) 424, 456, 771, 803
Bpu10I CCTNAGC 1 cut(s) 755
BpuEI CTTGAG 2 cut(s) 254, 438
BpuMI CCSGG 2 cut(s) 184, 1151
BsaAI YACGTR 1 cut(s) 542
BsaBI GATNNNNATC 1 cut(s) 911
BsaJI CCNNGG 2 cut(s) 507, 703
Bsc4I CCNNNNNNNGG 4 cut(s) 155, 178, 306, 686
Bse118I RCCGGY 3 cut(s) 113, 178, 287
Bse1I ACTGG 2 cut(s) 568, 1105
Bse8I GATNNNNATC 1 cut(s) 911
BseDI CCNNGG 2 cut(s) 507, 703
BseGI GGATG 1 cut(s) 1081
BseJI GATNNNNATC 1 cut(s) 911
BseLI CCNNNNNNNGG 4 cut(s) 155, 178, 306, 686
BseMII CTCAG 3 cut(s) 443, 790, 1106
BseNI ACTGG 2 cut(s) 568, 1105
BseSI GKGCMC 1 cut(s) 547
BseX3I CGGCCG 1 cut(s) 180
BseXI GCAGC 7 cut(s) 115, 211, 537, 695, 763, 784, 950
BseYI CCCAGC 1 cut(s) 1071
Bsh1236I CGCG 3 cut(s) 509, 685, 687
Bsh1285I CGRYCG 1 cut(s) 183
BshFI GGCC 7 cut(s) 51, 182, 191, 201, 310, 389, 719
BshNI GGYRCC 1 cut(s) 863
BsiEI CGRYCG 1 cut(s) 183
BsiHKAI GWGCWC 2 cut(s) 547, 789
BsiSI CCGG 7 cut(s) 77, 114, 179, 183, 288, 554, 1151
BslFI GGGAC 2 cut(s) 110, 773
BslI CCNNNNNNNGG 4 cut(s) 155, 178, 306, 686
BsmFI GGGAC 2 cut(s) 110, 773
BsmI GAATGC 1 cut(s) 164
BsnI GGCC 7 cut(s) 51, 182, 191, 201, 310, 389, 719
Bsp1286I GDGCHC 2 cut(s) 547, 789
Bsp143I GATC 5 cut(s) 293, 400, 499, 801, 912
BspANI GGCC 7 cut(s) 51, 182, 191, 201, 310, 389, 719
BspCNI CTCAG 3 cut(s) 442, 789, 1105
BspFNI CGCG 3 cut(s) 509, 685, 687
BspLI GGNNCC 2 cut(s) 295, 865
BspMAI CTGCAG 1 cut(s) 381
BspPI GGATC 2 cut(s) 288, 301
BspT107I GGYRCC 1 cut(s) 863
BsrBI CCGCTC 1 cut(s) 1020
BsrFI RCCGGY 3 cut(s) 113, 178, 287
BsrI ACTGG 2 cut(s) 568, 1105
BssAI RCCGGY 3 cut(s) 113, 178, 287
BssECI CCNNGG 2 cut(s) 507, 703
BssMI GATC 5 cut(s) 293, 400, 499, 801, 912
BssNAI GTATAC 1 cut(s) 1124
BssSI CACGAG 1 cut(s) 38
Bst1107I GTATAC 1 cut(s) 1124
Bst2BI CACGAG 1 cut(s) 38
Bst4CI ACNGT 5 cut(s) 16, 272, 301, 660, 1037
BstAPI GCANNNNNTGC 1 cut(s) 376
BstBAI YACGTR 1 cut(s) 542
BstC8I GCNNGC 5 cut(s) 180, 391, 650, 1018, 1136
BstDEI CTNAG 6 cut(s) 429, 524, 755, 776, 1092, 1167
BstDSI CCRYGG 2 cut(s) 507, 703
BstF5I GGATG 1 cut(s) 1081
BstFNI CGCG 3 cut(s) 509, 685, 687
BstH2I RGCGCY 1 cut(s) 441
BstHHI GCGC 3 cut(s) 370, 440, 687
BstKTI GATC 5 cut(s) 296, 403, 502, 804, 915
BstMBI GATC 5 cut(s) 293, 400, 499, 801, 912
BstMCI CGRYCG 1 cut(s) 183
BstMWI GCNNNNNNNGC 5 cut(s) 188, 281, 367, 376, 395
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 2 cut(s) 182, 1149
BstSFI CTRYAG 1 cut(s) 377
BstSLI GKGCMC 1 cut(s) 547
BstUI CGCG 3 cut(s) 509, 685, 687
BstV1I GCAGC 7 cut(s) 115, 211, 537, 695, 763, 784, 950
BstX2I RGATCY 1 cut(s) 293
BstYI RGATCY 1 cut(s) 293
BstZ17I GTATAC 1 cut(s) 1124
BstZI CGGCCG 1 cut(s) 180
BsuI GTATCC 1 cut(s) 615
BsuRI GGCC 7 cut(s) 51, 182, 191, 201, 310, 389, 719
BtgI CCRYGG 2 cut(s) 507, 703
BtgZI GCGATG 1 cut(s) 715
BtsCI GGATG 1 cut(s) 1081
BtsIMutI CAGTG 2 cut(s) 21, 1112
Cac8I GCNNGC 5 cut(s) 180, 391, 650, 1018, 1136
CaiI CAGNNNCTG 1 cut(s) 638
CfoI GCGC 3 cut(s) 370, 440, 687
Cfr10I RCCGGY 3 cut(s) 113, 178, 287
Cfr13I GGNCC 4 cut(s) 189, 388, 635, 718
Cfr42I CCGCGG 1 cut(s) 510
CseI GACGC 1 cut(s) 700
Csp6I GTAC 5 cut(s) 12, 17, 230, 864, 1033
CviAII CATG 4 cut(s) 358, 974, 989, 1044
CviQI GTAC 5 cut(s) 12, 17, 230, 864, 1033
DdeI CTNAG 6 cut(s) 429, 524, 755, 776, 1092, 1167
DpnI GATC 5 cut(s) 295, 402, 501, 803, 914
DpnII GATC 5 cut(s) 293, 400, 499, 801, 912
EaeI YGGCCR 2 cut(s) 180, 199
EagI CGGCCG 1 cut(s) 180
EciI GGCGGA 2 cut(s) 306, 669
Ecl136II GAGCTC 1 cut(s) 787
EclXI CGGCCG 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 789
Eco32I GATATC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 635
Eco47III AGCGCT 1 cut(s) 439
Eco52I CGGCCG 1 cut(s) 180
Eco53kI GAGCTC 1 cut(s) 787
EcoICRI GAGCTC 1 cut(s) 787
EcoO109I RGGNCCY 1 cut(s) 635
EcoRI GAATTC 1 cut(s) 1080
EcoRV GATATC 1 cut(s) 487
EcoT38I GRGCYC 1 cut(s) 789
FaeI CATG 4 cut(s) 361, 977, 992, 1047
FaqI GGGAC 2 cut(s) 110, 773
FatI CATG 4 cut(s) 357, 973, 988, 1043
FauI CCCGC 4 cut(s) 398, 502, 959, 992
FauNDI CATATG 1 cut(s) 808
FblI GTMKAC 1 cut(s) 1123
FokI GGATG 1 cut(s) 1088
FriOI GRGCYC 1 cut(s) 789
GlaI GCGC 3 cut(s) 369, 439, 686
GsaI CCCAGC 1 cut(s) 1075
HaeII RGCGCY 1 cut(s) 441
HaeIII GGCC 7 cut(s) 51, 182, 191, 201, 310, 389, 719
HapII CCGG 7 cut(s) 77, 114, 179, 183, 288, 554, 1151
HgaI GACGC 1 cut(s) 700
HhaI GCGC 3 cut(s) 370, 440, 687
Hin1II CATG 4 cut(s) 361, 977, 992, 1047
Hin6I GCGC 3 cut(s) 368, 438, 685
HinP1I GCGC 3 cut(s) 368, 438, 685
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HinfI GANTC 5 cut(s) 98, 448, 676, 765, 837
HpaII CCGG 7 cut(s) 77, 114, 179, 183, 288, 554, 1151
HphI GGTGA 1 cut(s) 760
Hpy166II GTNNAC 6 cut(s) 19, 545, 565, 933, 1033, 1124
Hpy188I TCNGA 8 cut(s) 109, 305, 432, 519, 591, 675, 779, 1161
Hpy188III TCNNGA 2 cut(s) 455, 695
Hpy8I GTNNAC 6 cut(s) 19, 545, 565, 933, 1033, 1124
Hpy99I CGWCG 2 cut(s) 701, 1060
HpyAV CCTTC 7 cut(s) 300, 435, 596, 854, 949, 1017, 1149
HpyCH4III ACNGT 5 cut(s) 16, 272, 301, 660, 1037
HpyCH4IV ACGT 3 cut(s) 479, 541, 918
HpyCH4V TGCA 6 cut(s) 131, 240, 268, 361, 379, 545
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 281, 367, 376, 395
HpyF3I CTNAG 6 cut(s) 429, 524, 755, 776, 1092, 1167
HpySE526I ACGT 3 cut(s) 479, 541, 918
Hsp92II CATG 4 cut(s) 361, 977, 992, 1047
HspAI GCGC 3 cut(s) 368, 438, 685
KpnI GGTACC 1 cut(s) 867
KroI GCCGGC 1 cut(s) 178
KroNI GCCGGC 1 cut(s) 180
KspI CCGCGG 1 cut(s) 510
Kzo9I GATC 5 cut(s) 293, 400, 499, 801, 912
LmnI GCTCC 1 cut(s) 792
Lsp1109I GCAGC 7 cut(s) 115, 211, 537, 695, 763, 784, 950
MaeII ACGT 3 cut(s) 479, 541, 918
MaeIII GTNAC 2 cut(s) 766, 919
MalI GATC 5 cut(s) 295, 402, 501, 803, 914
MbiI CCGCTC 1 cut(s) 1020
MboI GATC 5 cut(s) 293, 400, 499, 801, 912
MboII GAAGA 3 cut(s) 160, 611, 866
MflI RGATCY 1 cut(s) 293
MhlI GDGCHC 2 cut(s) 547, 789
MlyI GAGTC 3 cut(s) 92, 774, 831
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 7 cut(s) 41, 159, 180, 362, 462, 599, 603
MroNI GCCGGC 1 cut(s) 178
MseI TTAA 1 cut(s) 32
MspA1I CMGCKG 4 cut(s) 128, 509, 550, 1071
MspI CCGG 7 cut(s) 77, 114, 179, 183, 288, 554, 1151
MspR9I CCNGG 2 cut(s) 184, 1151
Mva1269I GAATGC 1 cut(s) 164
MvnI CGCG 3 cut(s) 509, 685, 687
MwoI GCNNNNNNNGC 5 cut(s) 188, 281, 367, 376, 395
NaeI GCCGGC 1 cut(s) 180
NciI CCSGG 2 cut(s) 184, 1151
NdeI CATATG 1 cut(s) 808
NdeII GATC 5 cut(s) 293, 400, 499, 801, 912
NgoMIV GCCGGC 1 cut(s) 178
NlaIII CATG 4 cut(s) 361, 977, 992, 1047
NlaIV GGNNCC 2 cut(s) 295, 865
NmeAIII GCCGAG 1 cut(s) 808
NmuCI GTSAC 1 cut(s) 766
NspI RCATGY 1 cut(s) 361
PcsI WCGNNNNNNNCGW 1 cut(s) 732
PctI GAATGC 1 cut(s) 164
PdiI GCCGGC 1 cut(s) 180
PfeI GAWTC 2 cut(s) 448, 676
PflFI GACNNNGTC 3 cut(s) 560, 764, 989
PleI GAGTC 3 cut(s) 92, 773, 831
PpsI GAGTC 3 cut(s) 92, 773, 831
Ppu21I YACGTR 1 cut(s) 542
PpuMI RGGWCCY 1 cut(s) 635
Psp124BI GAGCTC 1 cut(s) 789
Psp1406I AACGTT 1 cut(s) 918
Psp5II RGGWCCY 1 cut(s) 635
PspFI CCCAGC 1 cut(s) 1071
PspN4I GGNNCC 2 cut(s) 295, 865
PspPI GGNCC 4 cut(s) 189, 388, 635, 718
PspPPI RGGWCCY 1 cut(s) 635
PstI CTGCAG 1 cut(s) 381
PstNI CAGNNNCTG 1 cut(s) 638
PsuI RGATCY 1 cut(s) 293
PsyI GACNNNGTC 3 cut(s) 560, 764, 989
PvuII CAGCTG 2 cut(s) 550, 1071
RsaI GTAC 5 cut(s) 13, 18, 231, 865, 1034
RsaNI GTAC 5 cut(s) 12, 17, 230, 864, 1033
SacI GAGCTC 1 cut(s) 789
SacII CCGCGG 1 cut(s) 510
SaqAI TTAA 1 cut(s) 32
Sau3AI GATC 5 cut(s) 293, 400, 499, 801, 912
Sau96I GGNCC 4 cut(s) 189, 388, 635, 718
SchI GAGTC 3 cut(s) 92, 774, 831
ScrFI CCNGG 2 cut(s) 184, 1151
SduI GDGCHC 2 cut(s) 547, 789
SfcI CTRYAG 1 cut(s) 377
SfiI GGCCNNNNNGGCC 1 cut(s) 188
Sfr303I CCGCGG 1 cut(s) 510
SgrAI CRCCGGYG 2 cut(s) 113, 287
SgrBI CCGCGG 1 cut(s) 510
SinI GGWCC 1 cut(s) 635
SmlI CTYRAG 2 cut(s) 233, 453
SmoI CTYRAG 2 cut(s) 233, 453
SstI GAGCTC 1 cut(s) 789
StyD4I CCNGG 2 cut(s) 182, 1149
TaaI ACNGT 5 cut(s) 16, 272, 301, 660, 1037
TaiI ACGT 3 cut(s) 482, 544, 921
TaqI TCGA 5 cut(s) 354, 531, 696, 813, 905
TauI GCSGC 5 cut(s) 120, 194, 204, 685, 827
TfiI GAWTC 2 cut(s) 448, 676
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 2 cut(s) 28, 1112
TseFI GTSAC 1 cut(s) 766
TseI GCWGC 7 cut(s) 128, 224, 550, 708, 751, 797, 963
Tsp45I GTSAC 1 cut(s) 766
TspDTI ATGAA 6 cut(s) 17, 208, 612, 872, 968, 1032
TspRI CASTG 2 cut(s) 28, 1112
Tth111I GACNNNGTC 3 cut(s) 560, 764, 989
VneI GTGCAC 1 cut(s) 543
VpaK11BI GGWCC 1 cut(s) 635
XapI RAATTY 3 cut(s) 103, 277, 1080
XceI RCATGY 1 cut(s) 361
XmiI GTMKAC 1 cut(s) 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.