pycom17g03040

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002210
Physical Location & Seq
Forward (+)
1996477 .. 1999824
3348 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3006 bp
ATGTTTTTTTTGAATTGGGAATGGTGGGGACGCGATCTACTATATCCCTGGAGGAGGATTTGGGTTCTGCACCTGGACCGGAGAAGCTTGAAGTTCCAGGGGAGAAGATCGATCAGGGAAGTTGTCAACCACTCGGGTGGGGGCCGGGCTGGCTCAGGTGGGTCCGGTCGGGGAAGTGCCGGCGGTGGAAGGAGTTTTGGGAACCTGAACGTTGATGTATCTGATGCTGAGGAGGGTTTGGTGAAGTTGAAAGGGAAGGGGAGTGTTGAGAAGTTGGAGTCGGTTTGGAGTAACGAGAAGAAGGTGGTGAGTGGCGGTGCTGAGGCGGAGAGTGGAGGGAAAGGAAGAGGGGTTGGAGAAAATGGCGGTTCTTTGGATGGGATTGGAGAAGTCCCGGATGGGACTGAGACTCGTGAGGCCGGGGCTGATGTGAACGGAGGGATTGAAGAGAATGGGAGCTGTTTGGATGGGATTGGAGAAGACCCGGATGGGAAAACTGATGAGATTACTGAGGACATGGATGATGAGGGACATGAGTTTCTTGTTGGGGACTTTGTGTGGGGAAAGATTAAGAGCCATCCGTGGTGGCCTGCACAAATTTGTGATCCTTCGGACGCTTCGGAGTATGCCCTGAAATTGAAGGCCAAAGACAGGCTCCTGGTGGCGTATTTTGGGGACGGAACATTTGCTTGGTGCAATTCTTCGCAGCTGAAACCGTTTGAGGAGAATTTTAGGGAGATGTCTCGGCAGAGTAGCTCGAAGGCGTTTGTTAATGCTGTGCAGCAGGCTGTGGATGAGGTTGGAAGGCTTGTGAGGTTGAAGATGAGTTGTATATGTGTAAAGGAAGAGTTCCTGGGTGAGGTTGGTCGGCCGTTGGCAGTAAATGCCGGAATTAAGGAAGGAGTTCGTGTGCCAGAAGGCAGGGTTGGGAAGCTTTTGGATCGTGTTTCTGAGCCAGCAGAGCTTCTTGCTGAGTTGAAACGTGTTGCAGAAGTTATGCCAATGTCTAGTGAGCTTGAGCTGAATGCTTTAAAGAGTTGGTTGTCTGCATTTTATTGTTCAAAAGGAGGCTATCGATTGCCTGTTTTCGTCGAAGCGCAGCCAGTTCCTGGTCTTGAGGATGATTGGAGGGAGGTGGATGTCCCGGTCCAAGGGCCATTTGAAGACTGGTTTTCTTCTCCCAGAAAAACAGGACAAACTGATCAGCCTTTAAACGAAAGCTCAGCTCAAGGTTTAGAGAATAGGCAACACCAAAGAAGGAAGCAGAAGAGCATTGCTGATCTTATGGAAGAAGACGATGATATTCAGGCGGAAACTAAGGAAGGAGCAACCTCAGAGAAAGCAGGGGCATCATCTGGACAGAACAAGCGGAAAGGTGGCGAGAATCATAGTGAGAGTAATTTGACTTCTGAATCCGGAAAGAGAAGGGCCAAGCTTTCAAAGACGCCAACAAGCTCGCAGATGAAAAAACTTTCTAGTGTTGAAAATGGTGCTAGTGAAACTAAGAAGGGTGTCTTAACAAGGAGCAGGAAGAAGGATGAACGTATTGCAATTGATGGCAATGGTGGCGAGACCAAAGAAGAAGCTGGCGATAGTCCTGCTTCAAGAGACGAGGAATTGCGCAGTGGCGGTTCACAAACTGACATGAAGGACCAAATTGATCACCCCTCTTCGACAAGAGAGAGGAAAAGGAGCAAGTACTTGTCCCCTCCCTTCATAAATCTAAAGACTGGAAAAAGGAGTCTAGACATAGAAGTAGAATCTCTGAAAGTTTCTAATGACAACCTTGTTGGGTCACCGAAGATGCTGAGTCCTTTCGCGGAGACATTACAGAAGAAAGACTCTACAGAACTTATTGGGAATGAGATAACTGGTGGCTCGAGTTCGAAAAAACCTTCAGAAGATGAGAAGAGCATTGATCCGATGAAAGCTAATGTATCTACTCATAAAGTGCTATCTGGACTCCGCTCTGCAGCTGTTAATCCATCATCTCGAGTAGAAAAGAAATCCTTTAAGATTGTTGGGGATTTCGTGGCCATATTCAGAGACTCGATTTATCACAATGGCTCCAACTACGAACTGTACAAGAAGAAGCAACCGCATAAGAAGAGAAAGAAGTTGGAATCTGAACCTGGCTCAATGGGGAAAGACCGAAATCAGATCACAGAAAAGCAAACTGAATCTGGGAAGAAGAGAACCAAGAAGAGCAGTGAGACAAAGTCCGATAAGTCTACACCAGGGCAAGCTACTGAGACATCAGGTTCAGAGCCTGGTAAGCGAAAATCAAAGAATGCTTCTGGAACTCCGGATTTGAAGAAAAGGCGTAAGAAGACCGATGAAACAGCTTCACCGGCATCCCTTTTTGTGACATTTGGCCCTGGGTCCTCTCTCCCCACAAAATCCGATCTTATCAAGATTTATAGTAAATTCGGAGAGCTGGACGAAACAGAAACAGAGATGTTCTACACCAATTTCTGTGCTCGTGTTTCCTTTGTAAAATTTGCCGATGCACAAGAGGCCTTCAACCATTCGCAAAATGACAGTCCGTTCGGAGCTGCCAATGTCACTTTCCGGCTTCATAATCTGGCAGCTGCTTCGAAGGTTCGCGAGCTGAGTGAAATATCCAATTCTGCTCCTGCCAAGAAGTCCAGAGGCAAGACCAGAACCCAGGCATTAGCTTCACAGCCACCCGCCGCTGTTGGCGAGGCGTCACAAGTCGACTTAATCAAGCAGAAACTGGAGAGGATGACCTCAATGCTGGGTGATTCAAATGGCCAAGTGTCGGATGTGACGAAATCGAAACTGGAGAGCGAGATAAAGGAGCTGTTGGGGACGGTAAGCACGATGTCATGTGATATAGTTAGTGAGCGAGCGCGTATGCAGGCGAAGTTAGTATATCTCCATGTAACTAGTTGCTTTGTTCAGTTTTCTTCGGTCTTCCGAACGGAACGACGCGCAAACTTTCGAGGAGATGCAGAAGCAACATGGATAATGTTTCGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1002

Amino Acids

109.8

Weight (kDa)

8.63

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 183 - 266 4e-22 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1622
AccB7I CCANNNNNTGG 1 cut(s) 1109
AccBSI CCGCTC 1 cut(s) 1968
AccI GTMKAC 2 cut(s) 2231, 2718
AccII CGCG 5 cut(s) 33, 1820, 2607, 2875, 2955
AccIII TCCGGA 2 cut(s) 1415, 2305
AclI AACGTT 1 cut(s) 210
AclWI GGATC 3 cut(s) 599, 948, 1913
AcoI YGGCCR 3 cut(s) 869, 2034, 2773
AcsI RAATTY 4 cut(s) 597, 727, 2426, 2498
AcuI CTGAAG 1 cut(s) 1881
AcyI GRCGYC 2 cut(s) 1445, 2708
AfaI GTAC 2 cut(s) 1700, 2084
AfiI CCNNNNNNNGG 6 cut(s) 54, 651, 859, 1109, 1151, 2782
AflIII ACRYGT 1 cut(s) 982
AhlI ACTAGT 1 cut(s) 2909
AjuI GAANNNNNNNTTGG 6 cut(s) 673, 705, 909, 941, 2552, 2584
AleI CACNNNNGTG 1 cut(s) 135
Alw21I GWGCWC 1 cut(s) 2482
Alw26I GTCTC 8 cut(s) 401, 747, 1565, 1602, 1817, 2040, 2207, 2246
AlwI GGATC 3 cut(s) 599, 948, 1913
AlwNI CAGNNNCTG 3 cut(s) 1109, 2270, 2737
Ama87I CYCGRG 3 cut(s) 133, 1879, 1992
Aor13HI TCCGGA 2 cut(s) 1415, 2305
ApeKI GCWGC 7 cut(s) 706, 781, 1099, 1973, 2555, 2588, 2591
ApoI RAATTY 4 cut(s) 597, 727, 2426, 2498
ArsI GACNNNNNNTTYG 6 cut(s) 668, 700, 2527, 2531, 2559, 2563
Asp700I GAANNNNTTC 1 cut(s) 903
AspLEI GCGC 4 cut(s) 1099, 1623, 2875, 2957
AspS9I GGNCC 9 cut(s) 76, 142, 162, 1147, 1154, 1428, 1651, 2375, 2382
AsuC2I CCSGG 5 cut(s) 146, 395, 421, 485, 1145
AsuHPI GGTGA 7 cut(s) 253, 319, 869, 1655, 1788, 2340, 2774
AsuII TTCGAA 2 cut(s) 1886, 2597
AvaI CYCGRG 3 cut(s) 133, 1879, 1992
AvaII GGWCC 5 cut(s) 76, 162, 1147, 1651, 2382
BalI TGGCCA 2 cut(s) 2036, 2775
BarI GAAGNNNNNNTAC 2 cut(s) 1390, 1422
BauI CACGAG 2 cut(s) 411, 2481
BbsI GAAGAC 5 cut(s) 486, 1170, 1299, 2336, 2929
Bbv12I GWGCWC 1 cut(s) 2482
BbvCI CCTCAGC 2 cut(s) 228, 321
BbvI GCAGC 7 cut(s) 718, 793, 1111, 1985, 2542, 2578, 2600
BccI CCATC 7 cut(s) 371, 392, 461, 482, 585, 1550, 1993
BceAI ACGGC 1 cut(s) 856
BcgI CGANNNNNNTGC 4 cut(s) 1580, 1614, 2577, 2611
BclI TGATCA 2 cut(s) 1201, 1660
BcnI CCSGG 5 cut(s) 146, 395, 421, 485, 1145
BcoDI GTCTC 8 cut(s) 401, 747, 1565, 1602, 1817, 2040, 2207, 2246
BcuI ACTAGT 1 cut(s) 2909
BfaI CTAG 5 cut(s) 1008, 1476, 1494, 1745, 2910
BfmI CTRYAG 2 cut(s) 1845, 1971
BglI GCCNNNNNGGC 1 cut(s) 150
BisI GCNGC 8 cut(s) 707, 782, 1100, 1974, 2556, 2589, 2592, 2694
BlpI GCTNAGC 1 cut(s) 1222
BlsI GCNGC 8 cut(s) 708, 783, 1101, 1975, 2557, 2590, 2593, 2695
BmcAI AGTACT 1 cut(s) 1700
Bme18I GGWCC 5 cut(s) 76, 162, 1147, 1651, 2382
BmeT110I CYCGRG 3 cut(s) 133, 1879, 1992
BmgT120I GGNCC 9 cut(s) 76, 142, 162, 1147, 1154, 1428, 1651, 2375, 2382
BmiI GGNNCC 6 cut(s) 143, 163, 203, 656, 2068, 2383
BmsI GCATC 6 cut(s) 214, 1358, 1794, 2363, 2497, 2962
BpiI GAAGAC 5 cut(s) 486, 1170, 1299, 2336, 2929
BpmI CTGGAG 3 cut(s) 70, 2759, 2825
Bpu10I CCTNAGC 3 cut(s) 154, 228, 321
Bpu1102I GCTNAGC 1 cut(s) 1222
Bpu14I TTCGAA 2 cut(s) 1886, 2597
BpuEI CTTGAG 3 cut(s) 1037, 1136, 1212
BpuMI CCSGG 5 cut(s) 146, 395, 421, 485, 1145
Bsa29I ATCGAT 2 cut(s) 110, 1075
BsaHI GRCGYC 2 cut(s) 1445, 2708
BsaI GGTCTC 1 cut(s) 1565
BsaWI WCCGGW 4 cut(s) 78, 164, 1415, 2305
BsaXI ACNNNNNCTCC 4 cut(s) 894, 924, 2051, 2081
Bsc4I CCNNNNNNNGG 6 cut(s) 54, 651, 859, 1109, 1151, 2782
Bse118I RCCGGY 2 cut(s) 179, 2350
Bse1I ACTGG 6 cut(s) 1103, 1172, 1735, 1876, 2742, 2808
Bse3DI GCAATG 2 cut(s) 1272, 1567
BseAI TCCGGA 2 cut(s) 1415, 2305
BseCI ATCGAT 2 cut(s) 110, 1075
BseLI CCNNNNNNNGG 6 cut(s) 54, 651, 859, 1109, 1151, 2782
BseMI GCAATG 2 cut(s) 1272, 1567
BseNI ACTGG 6 cut(s) 1103, 1172, 1735, 1876, 2742, 2808
BseRI GAGGAG 4 cut(s) 67, 245, 737, 2982
BseX3I CGGCCG 1 cut(s) 869
BseXI GCAGC 7 cut(s) 718, 793, 1111, 1985, 2542, 2578, 2600
BseYI CCCAGC 1 cut(s) 2758
BsgI GTGCAG 3 cut(s) 53, 576, 800
Bsh1236I CGCG 5 cut(s) 33, 1820, 2607, 2875, 2955
Bsh1285I CGRYCG 2 cut(s) 169, 872
BshVI ATCGAT 2 cut(s) 110, 1075
BsiEI CGRYCG 2 cut(s) 169, 872
BsiHKAI GWGCWC 1 cut(s) 2482
BsiHKCI CYCGRG 3 cut(s) 133, 1879, 1992
BslFI GGGAC 9 cut(s) 42, 377, 415, 543, 563, 689, 1127, 1690, 2845
BslI CCNNNNNNNGG 6 cut(s) 54, 651, 859, 1109, 1151, 2782
BsmAI GTCTC 8 cut(s) 401, 747, 1565, 1602, 1817, 2040, 2207, 2246
BsmBI CGTCTC 1 cut(s) 1602
BsmFI GGGAC 9 cut(s) 42, 377, 415, 543, 563, 689, 1127, 1690, 2845
BsmI GAATGC 2 cut(s) 1030, 2296
Bso31I GGTCTC 1 cut(s) 1565
BsoBI CYCGRG 3 cut(s) 133, 1879, 1992
Bsp119I TTCGAA 2 cut(s) 1886, 2597
Bsp1286I GDGCHC 1 cut(s) 2482
Bsp13I TCCGGA 2 cut(s) 1415, 2305
Bsp1407I TGTACA 1 cut(s) 2082
Bsp1720I GCTNAGC 1 cut(s) 1222
Bsp68I TCGCGA 1 cut(s) 2607
BspDI ATCGAT 2 cut(s) 110, 1075
BspEI TCCGGA 2 cut(s) 1415, 2305
BspFNI CGCG 5 cut(s) 33, 1820, 2607, 2875, 2955
BspLI GGNNCC 6 cut(s) 143, 163, 203, 656, 2068, 2383
BspMAI CTGCAG 1 cut(s) 1975
BspPI GGATC 3 cut(s) 599, 948, 1913
BspQI GCTCTTC 3 cut(s) 1262, 1904, 2198
BspT104I TTCGAA 2 cut(s) 1886, 2597
BspTNI GGTCTC 1 cut(s) 1565
BsrBI CCGCTC 1 cut(s) 1968
BsrDI GCAATG 2 cut(s) 1272, 1567
BsrFI RCCGGY 2 cut(s) 179, 2350
BsrGI TGTACA 1 cut(s) 2082
BsrI ACTGG 6 cut(s) 1103, 1172, 1735, 1876, 2742, 2808
BssAI RCCGGY 2 cut(s) 179, 2350
BssNI GRCGYC 2 cut(s) 1445, 2708
BssSI CACGAG 2 cut(s) 411, 2481
BssT1I CCWWGG 1 cut(s) 1150
Bst2BI CACGAG 2 cut(s) 411, 2481
Bst4CI ACNGT 4 cut(s) 717, 2082, 2543, 2836
Bst6I CTCTTC 9 cut(s) 340, 441, 840, 1262, 1675, 1904, 2102, 2186, 2198
BstACI GRCGYC 2 cut(s) 1445, 2708
BstAPI GCANNNNNTGC 1 cut(s) 884
BstAUI TGTACA 1 cut(s) 2082
BstBI TTCGAA 2 cut(s) 1886, 2597
BstDSI CCRYGG 1 cut(s) 581
BstEII GGTNACC 1 cut(s) 1794
BstENI CCTNNNNNAGG 2 cut(s) 52, 857
BstFNI CGCG 5 cut(s) 33, 1820, 2607, 2875, 2955
BstHHI GCGC 4 cut(s) 1099, 1623, 2875, 2957
BstMAI GTCTC 8 cut(s) 401, 747, 1565, 1602, 1817, 2040, 2207, 2246
BstMCI CGRYCG 2 cut(s) 169, 872
BstMWI GCNNNNNNNGC 7 cut(s) 150, 884, 961, 1566, 2275, 2351, 2516
BstPI GGTNACC 1 cut(s) 1794
BstSFI CTRYAG 2 cut(s) 1845, 1971
BstUI CGCG 5 cut(s) 33, 1820, 2607, 2875, 2955
BstV1I GCAGC 7 cut(s) 718, 793, 1111, 1985, 2542, 2578, 2600
BstV2I GAAGAC 5 cut(s) 486, 1170, 1299, 2336, 2929
BstXI CCANNNNNNTGG 1 cut(s) 137
BstZI CGGCCG 1 cut(s) 869
Bsu15I ATCGAT 2 cut(s) 110, 1075
BsuTUI ATCGAT 2 cut(s) 110, 1075
BtgI CCRYGG 1 cut(s) 581
BtsI GCAGTG 2 cut(s) 1630, 2215
BtsIMutI CAGTG 2 cut(s) 1630, 2215
BtuMI TCGCGA 1 cut(s) 2607
CaiI CAGNNNCTG 3 cut(s) 1109, 2270, 2737
CfoI GCGC 4 cut(s) 1099, 1623, 2875, 2957
Cfr10I RCCGGY 2 cut(s) 179, 2350
Cfr13I GGNCC 9 cut(s) 76, 142, 162, 1147, 1154, 1428, 1651, 2375, 2382
ClaI ATCGAT 2 cut(s) 110, 1075
CseI GACGC 5 cut(s) 39, 623, 1453, 2697, 2961
Csp6I GTAC 2 cut(s) 1699, 2083
CviAII CATG 6 cut(s) 517, 533, 1645, 2850, 2903, 2985
CviQI GTAC 2 cut(s) 1699, 2083
DraI TTTAAA 2 cut(s) 1032, 1212
EaeI YGGCCR 3 cut(s) 869, 2034, 2773
EagI CGGCCG 1 cut(s) 869
Eam1104I CTCTTC 9 cut(s) 340, 441, 840, 1262, 1675, 1904, 2102, 2186, 2198
EarI CTCTTC 9 cut(s) 340, 441, 840, 1262, 1675, 1904, 2102, 2186, 2198
EciI GGCGGA 2 cut(s) 341, 1325
EclXI CGGCCG 1 cut(s) 869
Eco130I CCWWGG 1 cut(s) 1150
Eco147I AGGCCT 1 cut(s) 2519
Eco31I GGTCTC 1 cut(s) 1565
Eco47I GGWCC 5 cut(s) 76, 162, 1147, 1651, 2382
Eco52I CGGCCG 1 cut(s) 869
Eco57I CTGAAG 1 cut(s) 1881
Eco88I CYCGRG 3 cut(s) 133, 1879, 1992
Eco91I GGTNACC 1 cut(s) 1794
EcoNI CCTNNNNNAGG 2 cut(s) 52, 857
EcoO109I RGGNCCY 1 cut(s) 2382
EcoO65I GGTNACC 1 cut(s) 1794
EcoT14I CCWWGG 1 cut(s) 1150
ErhI CCWWGG 1 cut(s) 1150
Esp3I CGTCTC 1 cut(s) 1602
FaeI CATG 6 cut(s) 520, 536, 1648, 2853, 2906, 2988
FalI AAGNNNNNCTT 4 cut(s) 1499, 1531, 1994, 2026
FaqI GGGAC 9 cut(s) 42, 377, 415, 543, 563, 689, 1127, 1690, 2845
FatI CATG 6 cut(s) 516, 532, 1644, 2849, 2902, 2984
FauI CCCGC 1 cut(s) 2698
FbaI TGATCA 2 cut(s) 1201, 1660
FblI GTMKAC 2 cut(s) 2231, 2718
Fnu4HI GCNGC 8 cut(s) 707, 782, 1100, 1974, 2556, 2589, 2592, 2694
Fsp4HI GCNGC 8 cut(s) 707, 782, 1100, 1974, 2556, 2589, 2592, 2694
FspBI CTAG 5 cut(s) 1008, 1476, 1494, 1745, 2910
FspI TGCGCA 1 cut(s) 1622
GlaI GCGC 4 cut(s) 1098, 1622, 2874, 2956
GluI GCNGC 8 cut(s) 707, 782, 1100, 1974, 2556, 2589, 2592, 2694
GsaI CCCAGC 1 cut(s) 2762
GsuI CTGGAG 3 cut(s) 70, 2759, 2825
HgaI GACGC 5 cut(s) 39, 623, 1453, 2697, 2961
HhaI GCGC 4 cut(s) 1099, 1623, 2875, 2957
Hin1I GRCGYC 2 cut(s) 1445, 2708
Hin1II CATG 6 cut(s) 520, 536, 1648, 2853, 2906, 2988
Hin6I GCGC 4 cut(s) 1097, 1621, 2873, 2955
HinP1I GCGC 4 cut(s) 1097, 1621, 2873, 2955
HincII GTYRAC 2 cut(s) 127, 2719
HindII GTYRAC 2 cut(s) 127, 2719
HindIII AAGCTT 3 cut(s) 85, 932, 1433
HphI GGTGA 7 cut(s) 253, 319, 869, 1655, 1788, 2340, 2774
Hpy166II GTNNAC 5 cut(s) 127, 433, 1634, 2232, 2719
Hpy8I GTNNAC 5 cut(s) 127, 433, 1634, 2232, 2719
Hpy99I CGWCG 2 cut(s) 1094, 2955
HpyCH4III ACNGT 4 cut(s) 717, 2082, 2543, 2836
HpyCH4IV ACGT 3 cut(s) 210, 982, 1543
HpyF10VI GCNNNNNNNGC 7 cut(s) 150, 884, 961, 1566, 2275, 2351, 2516
HpySE526I ACGT 3 cut(s) 210, 982, 1543
Hsp92I GRCGYC 2 cut(s) 1445, 2708
Hsp92II CATG 6 cut(s) 520, 536, 1648, 2853, 2906, 2988
HspAI GCGC 4 cut(s) 1097, 1621, 2873, 2955
Kpn2I TCCGGA 2 cut(s) 1415, 2305
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
Ksp22I TGATCA 2 cut(s) 1201, 1660
LguI GCTCTTC 3 cut(s) 1262, 1904, 2198
LmnI GCTCC 9 cut(s) 456, 660, 1325, 1524, 1692, 2072, 2552, 2638, 2821
Lsp1109I GCAGC 7 cut(s) 718, 793, 1111, 1985, 2542, 2578, 2600
LweI GCATC 6 cut(s) 214, 1358, 1794, 2363, 2497, 2962
MaeI CTAG 5 cut(s) 1008, 1476, 1494, 1745, 2910
MaeII ACGT 3 cut(s) 210, 982, 1543
MaeIII GTNAC 7 cut(s) 290, 1794, 2365, 2563, 2709, 2788, 2905
MbiI CCGCTC 1 cut(s) 1968
MfeI CAATTG 1 cut(s) 1551
MhlI GDGCHC 1 cut(s) 2482
MlsI TGGCCA 2 cut(s) 2036, 2775
MluNI TGGCCA 2 cut(s) 2036, 2775
MlyI GAGTC 7 cut(s) 287, 403, 1750, 1819, 1835, 1956, 2042
MmeI TCCRAC 6 cut(s) 255, 334, 781, 2094, 2100, 2763
Mox20I TGGCCA 2 cut(s) 2036, 2775
MroI TCCGGA 2 cut(s) 1415, 2305
MroNI GCCGGC 1 cut(s) 179
MroXI GAANNNNTTC 1 cut(s) 903
MscI TGGCCA 2 cut(s) 2036, 2775
MseI TTAA 9 cut(s) 570, 771, 894, 1031, 1211, 1517, 1980, 2013, 2723
MslI CAYNNNNRTG 1 cut(s) 135
Msp20I TGGCCA 2 cut(s) 2036, 2775
MspA1I CMGCKG 4 cut(s) 709, 1976, 2591, 2696
MunI CAATTG 1 cut(s) 1551
Mva1269I GAATGC 2 cut(s) 1030, 2296
MvnI CGCG 5 cut(s) 33, 1820, 2607, 2875, 2955
MwoI GCNNNNNNNGC 7 cut(s) 150, 884, 961, 1566, 2275, 2351, 2516
NaeI GCCGGC 1 cut(s) 181
NciI CCSGG 5 cut(s) 146, 395, 421, 485, 1145
NgoMIV GCCGGC 1 cut(s) 179
NlaIII CATG 6 cut(s) 520, 536, 1648, 2853, 2906, 2988
NlaIV GGNNCC 6 cut(s) 143, 163, 203, 656, 2068, 2383
NmeAIII GCCGAG 1 cut(s) 724
NmuCI GTSAC 5 cut(s) 1794, 2365, 2563, 2709, 2788
NruI TCGCGA 1 cut(s) 2607
NsbI TGCGCA 1 cut(s) 1622
NspV TTCGAA 2 cut(s) 1886, 2597
OliI CACNNNNGTG 1 cut(s) 135
PaeR7I CTCGAG 2 cut(s) 1879, 1992
PasI CCCWGGG 1 cut(s) 2378
PceI AGGCCT 1 cut(s) 2519
PciSI GCTCTTC 3 cut(s) 1262, 1904, 2198
PcsI WCGNNNNNNNCGW 2 cut(s) 2789, 2840
PctI GAATGC 2 cut(s) 1030, 2296
PdiI GCCGGC 1 cut(s) 181
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 6 cut(s) 1384, 1412, 1760, 2123, 2180, 2765
PflFI GACNNNGTC 1 cut(s) 2218
PflMI CCANNNNNTGG 1 cut(s) 1109
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 8 cut(s) 708, 783, 1101, 1975, 2557, 2590, 2593, 2695
PleI GAGTC 7 cut(s) 286, 403, 1749, 1818, 1835, 1956, 2042
PpsI GAGTC 7 cut(s) 286, 403, 1749, 1818, 1835, 1956, 2042
PpuMI RGGWCCY 1 cut(s) 2382
Psp1406I AACGTT 1 cut(s) 210
Psp5II RGGWCCY 1 cut(s) 2382
PspEI GGTNACC 1 cut(s) 1794
PspFI CCCAGC 1 cut(s) 2758
PspN4I GGNNCC 6 cut(s) 143, 163, 203, 656, 2068, 2383
PspPI GGNCC 9 cut(s) 76, 142, 162, 1147, 1154, 1428, 1651, 2375, 2382
PspPPI RGGWCCY 1 cut(s) 2382
PspXI VCTCGAGB 1 cut(s) 1879
PstI CTGCAG 1 cut(s) 1975
PstNI CAGNNNCTG 3 cut(s) 1109, 2270, 2737
PsyI GACNNNGTC 1 cut(s) 2218
PvuII CAGCTG 3 cut(s) 709, 1976, 2591
RruI TCGCGA 1 cut(s) 2607
RsaI GTAC 2 cut(s) 1700, 2084
RsaNI GTAC 2 cut(s) 1699, 2083
RseI CAYNNNNRTG 1 cut(s) 135
SalI GTCGAC 1 cut(s) 2717
SapI GCTCTTC 3 cut(s) 1262, 1904, 2198
SaqAI TTAA 9 cut(s) 570, 771, 894, 1031, 1211, 1517, 1980, 2013, 2723
SatI GCNGC 8 cut(s) 707, 782, 1100, 1974, 2556, 2589, 2592, 2694
Sau96I GGNCC 9 cut(s) 76, 142, 162, 1147, 1154, 1428, 1651, 2375, 2382
ScaI AGTACT 1 cut(s) 1700
SchI GAGTC 7 cut(s) 287, 403, 1750, 1819, 1835, 1956, 2042
SduI GDGCHC 1 cut(s) 2482
SfaNI GCATC 6 cut(s) 214, 1358, 1794, 2363, 2497, 2962
SfcI CTRYAG 2 cut(s) 1845, 1971
Sfr274I CTCGAG 2 cut(s) 1879, 1992
SfuI TTCGAA 2 cut(s) 1886, 2597
SinI GGWCC 5 cut(s) 76, 162, 1147, 1651, 2382
SlaI CTCGAG 2 cut(s) 1879, 1992
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 5 cut(s) 1016, 1115, 1227, 1879, 1992
SmoI CTYRAG 5 cut(s) 1016, 1115, 1227, 1879, 1992
SpeI ACTAGT 1 cut(s) 2909
SseBI AGGCCT 1 cut(s) 2519
SspMI CTAG 5 cut(s) 1008, 1476, 1494, 1745, 2910
StuI AGGCCT 1 cut(s) 2519
StyI CCWWGG 1 cut(s) 1150
TaaI ACNGT 4 cut(s) 717, 2082, 2543, 2836
TaiI ACGT 3 cut(s) 213, 985, 1546
TaqII GACCGA 3 cut(s) 2166, 2348, 2923
TatI WGTACW 2 cut(s) 1698, 2082
TauI GCSGC 1 cut(s) 2696
TfiI GAWTC 6 cut(s) 1384, 1412, 1760, 2123, 2180, 2765
Tru1I TTAA 9 cut(s) 570, 771, 894, 1031, 1211, 1517, 1980, 2013, 2723
Tru9I TTAA 9 cut(s) 570, 771, 894, 1031, 1211, 1517, 1980, 2013, 2723
TscAI CASTG 2 cut(s) 1630, 2215
TseFI GTSAC 5 cut(s) 1794, 2365, 2563, 2709, 2788
TseI GCWGC 7 cut(s) 706, 781, 1099, 1973, 2555, 2588, 2591
Tsp45I GTSAC 5 cut(s) 1794, 2365, 2563, 2709, 2788
TspDTI ATGAA 7 cut(s) 1478, 1554, 1661, 1705, 1940, 2352, 2567
TspGWI ACGGA 5 cut(s) 450, 570, 693, 2535, 2960
TspRI CASTG 2 cut(s) 1630, 2215
Tth111I GACNNNGTC 1 cut(s) 2218
Van91I CCANNNNNTGG 1 cut(s) 1109
VpaK11BI GGWCC 5 cut(s) 76, 162, 1147, 1651, 2382
XagI CCTNNNNNAGG 2 cut(s) 52, 857
XapI RAATTY 4 cut(s) 597, 727, 2426, 2498
XbaI TCTAGA 1 cut(s) 1744
XcmI CCANNNNNNNNNTGG 1 cut(s) 1164
XhoI CTCGAG 2 cut(s) 1879, 1992
XmiI GTMKAC 2 cut(s) 2231, 2718
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 5 cut(s) 1008, 1476, 1494, 1745, 2910
ZrmI AGTACT 1 cut(s) 1700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.