RchiOBHm_Chr6g0312401

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002236
Physical Location & Seq
Reverse (-)
69008605 .. 69011004
2400 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 498 bp
ATGGCTGTGACGGTGAAGCAGATGGCTATGATAGTGGCCTTTTTTGGTGTACTGTCTTTCATCTTCGGGATTATTGCGGAAAACAAGAAGCTGGAAAACCAGTGCCGTGGAAAGATGTTGTTATTTGCAAGGAAGTCCCTTCCGCAAGCTGCTTTGTTCCGGAGCAAGAGTTTCGTCGTCTTCTTCAACATTTCTTTGCTTACGGCAGGACTAGCTGCAGCTCTGTTGCTGTGGACTACAATAACGGAGCAACTTCATCTATCACGAAATGTCCATCACAATCTTAAAACAGACTGCCCCACTGCTAAGACTGGTCTCCTTGGTGGCGGTGCATTTGTATCCCTTGATTCAGCCCTTTTCTGGTTGGTTGCCCTCATGCTGGCCGACAATGCCCGGGAGGACTACTTTGAGGAGTCGGATAAGGATGTGAAGGGTAGTGGGTATACAGAGGTACACGGAGCTGATTATGGACAACATGTTGTAAAAGGTAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

165

Amino Acids

18.03

Weight (kDa)

8.57

Isoelectric Point (pI)

28.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 44 - 119 8.7e-17 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 443
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 3 cut(s) 77, 143, 327
AcoI YGGCCR 1 cut(s) 381
AfaI GTAC 2 cut(s) 51, 453
AfiI CCNNNNNNNGG 2 cut(s) 360, 379
AflIII ACRYGT 1 cut(s) 475
AgsI TTSAA 1 cut(s) 187
AluBI AGCT 5 cut(s) 91, 149, 215, 221, 461
AluI AGCT 5 cut(s) 91, 149, 215, 221, 461
Alw26I GTCTC 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 393
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 2 cut(s) 36, 381
ApeKI GCWGC 3 cut(s) 149, 215, 218
AsuC2I CCSGG 2 cut(s) 394, 395
AsuHPI GGTGA 1 cut(s) 25
AvaI CYCGRG 1 cut(s) 393
BbsI GAAGAC 1 cut(s) 172
BbvI GCAGC 3 cut(s) 136, 202, 230
BccI CCATC 2 cut(s) 16, 282
BceAI ACGGC 2 cut(s) 90, 219
BcgI CGANNNNNNTGC 2 cut(s) 154, 188
BciVI GTATCC 1 cut(s) 349
BcnI CCSGG 2 cut(s) 394, 395
BcoDI GTCTC 1 cut(s) 320
BfaI CTAG 1 cut(s) 212
BfmI CTRYAG 1 cut(s) 216
BfuI GTATCC 1 cut(s) 349
BisI GCNGC 3 cut(s) 150, 216, 219
BlsI GCNGC 3 cut(s) 151, 217, 220
Bme1390I CCNGG 2 cut(s) 394, 395
BmeT110I CYCGRG 1 cut(s) 393
BmrFI CCNGG 2 cut(s) 394, 395
BpiI GAAGAC 1 cut(s) 172
BpuMI CCSGG 2 cut(s) 394, 395
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 3 cut(s) 106, 319, 393
BsaWI WCCGGW 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bsc4I CCNNNNNNNGG 2 cut(s) 360, 379
Bse1I ACTGG 2 cut(s) 100, 316
BseAI TCCGGA 1 cut(s) 159
BseDI CCNNGG 3 cut(s) 106, 319, 393
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 2 cut(s) 360, 379
BseNI ACTGG 2 cut(s) 100, 316
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 3 cut(s) 136, 202, 230
BshFI GGCC 2 cut(s) 38, 383
BsiHKCI CYCGRG 1 cut(s) 393
BsiSI CCGG 2 cut(s) 160, 394
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 2 cut(s) 360, 379
BsmAI GTCTC 1 cut(s) 320
BsmFI GGGAC 1 cut(s) 121
BsnI GGCC 2 cut(s) 38, 383
Bso31I GGTCTC 1 cut(s) 320
BsoBI CYCGRG 1 cut(s) 393
Bsp13I TCCGGA 1 cut(s) 159
BspACI CCGC 3 cut(s) 77, 143, 327
BspANI GGCC 2 cut(s) 38, 383
BspEI TCCGGA 1 cut(s) 159
BspMAI CTGCAG 1 cut(s) 220
BspTNI GGTCTC 1 cut(s) 320
BsrI ACTGG 2 cut(s) 100, 316
BssECI CCNNGG 3 cut(s) 106, 319, 393
BssNAI GTATAC 1 cut(s) 444
BssT1I CCWWGG 1 cut(s) 319
Bst1107I GTATAC 1 cut(s) 444
Bst4CI ACNGT 2 cut(s) 13, 54
BstC8I GCNNGC 2 cut(s) 147, 381
BstDEI CTNAG 1 cut(s) 306
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 430
BstMAI GTCTC 1 cut(s) 320
BstMWI GCNNNNNNNGC 2 cut(s) 212, 389
BstNSI RCATGY 1 cut(s) 479
BstSCI CCNGG 2 cut(s) 392, 393
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 3 cut(s) 136, 202, 230
BstV2I GAAGAC 1 cut(s) 172
BstXI CCANNNNNNTGG 1 cut(s) 107
BstZ17I GTATAC 1 cut(s) 444
BsuI GTATCC 1 cut(s) 349
BsuRI GGCC 2 cut(s) 38, 383
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 300
BtsIMutI CAGTG 2 cut(s) 107, 300
Cac8I GCNNGC 2 cut(s) 147, 381
Cfr9I CCCGGG 1 cut(s) 393
Csp6I GTAC 2 cut(s) 50, 452
CviAII CATG 2 cut(s) 376, 476
CviQI GTAC 2 cut(s) 50, 452
DdeI CTNAG 1 cut(s) 306
EaeI YGGCCR 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 319
Eco31I GGTCTC 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 393
EcoT14I CCWWGG 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 2 cut(s) 379, 479
FaiI YATR 5 cut(s) 29, 377, 444, 468, 477
FaqI GGGAC 1 cut(s) 121
FatI CATG 2 cut(s) 375, 475
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 3 cut(s) 150, 216, 219
FokI GGATG 1 cut(s) 437
Fsp4HI GCNGC 3 cut(s) 150, 216, 219
FspBI CTAG 1 cut(s) 212
GluI GCNGC 3 cut(s) 150, 216, 219
HaeIII GGCC 2 cut(s) 38, 383
HapII CCGG 2 cut(s) 160, 394
Hin1II CATG 2 cut(s) 379, 479
HinfI GANTC 2 cut(s) 347, 413
HpaII CCGG 2 cut(s) 160, 394
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 4 cut(s) 50, 234, 444, 454
Hpy188I TCNGA 1 cut(s) 418
Hpy188III TCNNGA 3 cut(s) 67, 160, 264
Hpy8I GTNNAC 4 cut(s) 50, 234, 444, 454
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 2 cut(s) 149, 424
HpyCH4III ACNGT 2 cut(s) 13, 54
HpyCH4V TGCA 3 cut(s) 128, 218, 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 389
HpyF3I CTNAG 1 cut(s) 306
Hsp92II CATG 2 cut(s) 379, 479
Kpn2I TCCGGA 1 cut(s) 159
LmnI GCTCC 3 cut(s) 162, 247, 458
LpnPI CCDG 8 cut(s) 77, 113, 173, 192, 297, 346, 365, 407
Lsp1109I GCAGC 3 cut(s) 136, 202, 230
MaeI CTAG 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 7
MboII GAAGA 3 cut(s) 55, 172, 175
MlyI GAGTC 1 cut(s) 422
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 4 cut(s) 383, 391, 403, 442
MroI TCCGGA 1 cut(s) 159
MseI TTAA 1 cut(s) 285
MspI CCGG 2 cut(s) 160, 394
MspR9I CCNGG 2 cut(s) 394, 395
MwoI GCNNNNNNNGC 2 cut(s) 212, 389
NciI CCSGG 2 cut(s) 394, 395
NlaIII CATG 2 cut(s) 379, 479
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 479
PciI ACATGT 1 cut(s) 475
PfeI GAWTC 1 cut(s) 347
PkrI GCNGC 3 cut(s) 151, 217, 220
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
PscI ACATGT 1 cut(s) 475
PstI CTGCAG 1 cut(s) 220
RsaI GTAC 2 cut(s) 51, 453
RsaNI GTAC 2 cut(s) 50, 452
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 3 cut(s) 150, 216, 219
SchI GAGTC 1 cut(s) 422
ScrFI CCNGG 2 cut(s) 394, 395
SetI ASST 7 cut(s) 93, 151, 217, 223, 453, 463, 490
SfcI CTRYAG 1 cut(s) 216
SmaI CCCGGG 1 cut(s) 395
SsiI CCGC 3 cut(s) 77, 143, 327
SspMI CTAG 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 392, 393
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 2 cut(s) 13, 54
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 2 cut(s) 107, 307
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 3 cut(s) 149, 215, 218
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 2 cut(s) 49, 245
TspGWI ACGGA 2 cut(s) 260, 471
TspMI CCCGGG 1 cut(s) 393
TspRI CASTG 2 cut(s) 107, 307
XceI RCATGY 1 cut(s) 479
XmaI CCCGGG 1 cut(s) 393
XmiI GTMKAC 1 cut(s) 443
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.