RchiOBHm_Chr5g0065211

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002544
Physical Location & Seq
Reverse (-)
71075350 .. 71077076
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 798 bp
ATGGTTCCAGGAGAACTTCATCTTGGGAAATTGCATCAAGATTTACTGGACCTGCGCGGTCATTTTGAGAAGTTAGTCCTTGAGCTTCAAAAAGCTCTGTTAAAATCTCAAGAGCAAAAGAAAGATCTTGAGAAACAACTTGAGGAGAGCGAAAACAAAAGGGAAGAGCTTAACAAGCTAGTCCTGGACTATGAAGGTAATATCAAGGATCTTAAGGATGATCTTGAGCAACGTGAAGCTCGAACAAAGGATCCTAAGAATAGTGTTGATCAGAGTGAAGATGGCATTAAGAAAGAACTTTATCAGGTTGGCTTTTATGAGTCGAGGATTGAAAATATGACCAAAGAGCGTGATGGCTTAATTAACAAGTTGAAAAGCGTTGGAGCAGAGCTAAAGGAAGCCCAAGAGGACTTGGAATTAGGCAAGAAGGTTTGGGAGGTTGAGAGGATAGCACTGGCTGAGAGGCTTACTAATGCAAAGCAGGTGTGGGAGGCTGAGAGGATGGAACTGACTAAAAAGCTTAACCATGAGTTAAACAAGTTTGGAATAGAGATAAAGGAGCTCAGAGAGGAGTTGTCTGACTTGGAGAAGATCCACAAGCAGTACATTCAAGAAAATGAGAACGCATATTGCTCAGGGTACCTGACTGGTTGGTTCAAGGAGCCTCATGCCTACATTTCCCTGCCAAGCCATGAGGCCAAGGAAGTTACTCTTCTAGATCATGAACATCTGGAGGCCACGTCTCCACTCTCTCACACTAGTGCCTTAATTAAACTTTTCGGAAGTGCTGATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

265

Amino Acids

30.78

Weight (kDa)

5.25

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 472
Acc36I ACCTGC 2 cut(s) 60, 472
Acc65I GGTACC 1 cut(s) 639
AccB1I GGYRCC 1 cut(s) 639
AccII CGCG 1 cut(s) 57
AciI CCGC 1 cut(s) 57
AclWI GGATC 5 cut(s) 216, 245, 258, 586, 785
AfaI GTAC 2 cut(s) 605, 641
AflII CTTAAG 1 cut(s) 212
AgsI TTSAA 5 cut(s) 89, 332, 373, 611, 658
AhlI ACTAGT 1 cut(s) 758
AjiI CACGTC 1 cut(s) 741
AjnI CCWGG 2 cut(s) 7, 183
AjuI GAANNNNNNNTTGG 1 cut(s) 38
AleI CACNNNNGTG 1 cut(s) 759
AloI GAACNNNNNNTCC 2 cut(s) 235, 267
AluBI AGCT 8 cut(s) 85, 95, 169, 178, 239, 391, 520, 562
AluI AGCT 8 cut(s) 85, 95, 169, 178, 239, 391, 520, 562
Alw21I GWGCWC 1 cut(s) 564
Alw26I GTCTC 1 cut(s) 747
AlwI GGATC 5 cut(s) 216, 245, 258, 586, 785
AoxI GGCC 2 cut(s) 696, 735
Asp718I GGTACC 1 cut(s) 639
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 49
AvaII GGWCC 1 cut(s) 49
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 639
BanII GRGCYC 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 564
BccI CCATC 3 cut(s) 275, 347, 496
BciT130I CCWGG 2 cut(s) 9, 185
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 1 cut(s) 747
BcuI ACTAGT 1 cut(s) 758
BfaI CTAG 3 cut(s) 179, 716, 759
BfrI CTTAAG 1 cut(s) 212
BfuAI ACCTGC 2 cut(s) 60, 472
BglII AGATCT 1 cut(s) 124
Bme1390I CCNGG 2 cut(s) 9, 185
Bme18I GGWCC 1 cut(s) 49
BmgBI CACGTC 1 cut(s) 741
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 4 cut(s) 6, 252, 641, 663
BmrFI CCNGG 2 cut(s) 9, 185
BmsI GCATC 1 cut(s) 43
BpmI CTGGAG 1 cut(s) 752
Bpu10I CCTNAGC 1 cut(s) 634
BpuEI CTTGAG 5 cut(s) 93, 101, 149, 161, 245
BsaJI CCNNGG 1 cut(s) 699
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bse1I ACTGG 3 cut(s) 51, 459, 652
BseBI CCWGG 2 cut(s) 9, 185
BseDI CCNNGG 1 cut(s) 699
BseGI GGATG 2 cut(s) 223, 507
BseMII CTCAG 4 cut(s) 450, 486, 577, 648
BseNI ACTGG 3 cut(s) 51, 459, 652
BseRI GAGGAG 2 cut(s) 158, 584
Bsh1236I CGCG 1 cut(s) 57
BshFI GGCC 2 cut(s) 698, 737
BshNI GGYRCC 1 cut(s) 639
BsiHKAI GWGCWC 1 cut(s) 564
BsmAI GTCTC 1 cut(s) 747
BsmBI CGTCTC 1 cut(s) 747
BsnI GGCC 2 cut(s) 698, 737
Bsp1286I GDGCHC 1 cut(s) 564
Bsp143I GATC 8 cut(s) 124, 208, 220, 250, 268, 591, 718, 790
BspACI CCGC 1 cut(s) 57
BspANI GGCC 2 cut(s) 698, 737
BspCNI CTCAG 4 cut(s) 451, 487, 576, 647
BspFNI CGCG 1 cut(s) 57
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 4 cut(s) 6, 252, 641, 663
BspMI ACCTGC 2 cut(s) 60, 472
BspPI GGATC 5 cut(s) 216, 245, 258, 586, 785
BspQI GCTCTTC 1 cut(s) 159
BspT107I GGYRCC 1 cut(s) 639
BspTI CTTAAG 1 cut(s) 212
BsrI ACTGG 3 cut(s) 51, 459, 652
BssECI CCNNGG 1 cut(s) 699
BssMI GATC 8 cut(s) 124, 208, 220, 250, 268, 591, 718, 790
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 2 cut(s) 9, 185
Bst6I CTCTTC 2 cut(s) 159, 717
BstAFI CTTAAG 1 cut(s) 212
BstDEI CTNAG 5 cut(s) 255, 459, 495, 563, 634
BstF5I GGATG 2 cut(s) 223, 507
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 8 cut(s) 127, 211, 223, 253, 271, 594, 721, 793
BstMAI GTCTC 1 cut(s) 747
BstMBI GATC 8 cut(s) 124, 208, 220, 250, 268, 591, 718, 790
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 2 cut(s) 9, 185
BstSCI CCNGG 2 cut(s) 7, 183
BstUI CGCG 1 cut(s) 57
BstX2I RGATCY 4 cut(s) 124, 208, 250, 591
BstYI RGATCY 4 cut(s) 124, 208, 250, 591
BsuRI GGCC 2 cut(s) 698, 737
BtrI CACGTC 1 cut(s) 741
BtsCI GGATG 2 cut(s) 223, 507
BtsIMutI CAGTG 1 cut(s) 452
BveI ACCTGC 2 cut(s) 60, 472
CciI TCATGA 1 cut(s) 721
CfoI GCGC 1 cut(s) 57
Cfr13I GGNCC 1 cut(s) 49
Csp6I GTAC 2 cut(s) 604, 640
CviAII CATG 4 cut(s) 527, 668, 692, 722
CviQI GTAC 2 cut(s) 604, 640
DdeI CTNAG 5 cut(s) 255, 459, 495, 563, 634
DpnI GATC 8 cut(s) 126, 210, 222, 252, 270, 593, 720, 792
DpnII GATC 8 cut(s) 124, 208, 220, 250, 268, 591, 718, 790
Eam1104I CTCTTC 2 cut(s) 159, 717
EarI CTCTTC 2 cut(s) 159, 717
Ecl136II GAGCTC 1 cut(s) 562
Eco130I CCWWGG 1 cut(s) 699
Eco24I GRGCYC 1 cut(s) 564
Eco47I GGWCC 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 562
EcoICRI GAGCTC 1 cut(s) 562
EcoRII CCWGG 2 cut(s) 7, 183
EcoT14I CCWWGG 1 cut(s) 699
EcoT38I GRGCYC 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 699
Esp3I CGTCTC 1 cut(s) 747
FaeI CATG 4 cut(s) 530, 671, 695, 725
FaiI YATR 9 cut(s) 192, 318, 338, 528, 628, 669, 693, 723, 796
FalI AAGNNNNNCTT 2 cut(s) 696, 728
FatI CATG 4 cut(s) 526, 667, 691, 721
FbaI TGATCA 1 cut(s) 268
FokI GGATG 2 cut(s) 230, 514
FriOI GRGCYC 1 cut(s) 564
FspBI CTAG 3 cut(s) 179, 716, 759
GlaI GCGC 1 cut(s) 56
GsuI CTGGAG 1 cut(s) 752
HaeIII GGCC 2 cut(s) 698, 737
HhaI GCGC 1 cut(s) 57
Hin1II CATG 4 cut(s) 530, 671, 695, 725
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 1 cut(s) 320
Hpy188I TCNGA 4 cut(s) 273, 566, 580, 782
Hpy188III TCNNGA 8 cut(s) 38, 110, 128, 224, 611, 716, 722, 731
HpyAV CCTTC 2 cut(s) 188, 421
HpyCH4IV ACGT 2 cut(s) 232, 740
HpyCH4V TGCA 2 cut(s) 34, 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 5 cut(s) 255, 459, 495, 563, 634
HpySE526I ACGT 2 cut(s) 232, 740
Hsp92II CATG 4 cut(s) 530, 671, 695, 725
HspAI GCGC 1 cut(s) 55
KpnI GGTACC 1 cut(s) 643
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 8 cut(s) 124, 208, 220, 250, 268, 591, 718, 790
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 3 cut(s) 383, 559, 661
LweI GCATC 1 cut(s) 43
MaeI CTAG 3 cut(s) 179, 716, 759
MaeII ACGT 2 cut(s) 232, 740
MaeIII GTNAC 1 cut(s) 706
MalI GATC 8 cut(s) 126, 210, 222, 252, 270, 593, 720, 792
MboI GATC 8 cut(s) 124, 208, 220, 250, 268, 591, 718, 790
MboII GAAGA 4 cut(s) 176, 290, 601, 704
MflI RGATCY 4 cut(s) 124, 208, 250, 591
MhlI GDGCHC 1 cut(s) 564
MluCI AATT 4 cut(s) 29, 360, 416, 768
MlyI GAGTC 1 cut(s) 329
MmeI TCCRAC 1 cut(s) 361
MslI CAYNNNNRTG 1 cut(s) 759
MspCI CTTAAG 1 cut(s) 212
MspR9I CCNGG 2 cut(s) 9, 185
MvaI CCWGG 2 cut(s) 9, 185
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 175
NdeII GATC 8 cut(s) 124, 208, 220, 250, 268, 591, 718, 790
NlaIII CATG 4 cut(s) 530, 671, 695, 725
NlaIV GGNNCC 4 cut(s) 6, 252, 641, 663
OliI CACNNNNGTG 1 cut(s) 759
PacI TTAATTAA 2 cut(s) 363, 771
PagI TCATGA 1 cut(s) 721
PaqCI CACCTGC 1 cut(s) 472
PciSI GCTCTTC 1 cut(s) 159
PcsI WCGNNNNNNNCGW 1 cut(s) 238
PfoI TCCNGGA 2 cut(s) 7, 183
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
Psp124BI GAGCTC 1 cut(s) 564
Psp6I CCWGG 2 cut(s) 7, 183
PspGI CCWGG 2 cut(s) 7, 183
PspN4I GGNNCC 4 cut(s) 6, 252, 641, 663
PspPI GGNCC 1 cut(s) 49
PsuI RGATCY 4 cut(s) 124, 208, 250, 591
RsaI GTAC 2 cut(s) 605, 641
RsaNI GTAC 2 cut(s) 604, 640
RseI CAYNNNNRTG 1 cut(s) 759
SacI GAGCTC 1 cut(s) 564
SapI GCTCTTC 1 cut(s) 159
Sau3AI GATC 8 cut(s) 124, 208, 220, 250, 268, 591, 718, 790
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 9, 185
SduI GDGCHC 1 cut(s) 564
SfaNI GCATC 1 cut(s) 43
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 759
SmlI CTYRAG 6 cut(s) 80, 108, 128, 140, 212, 224
SmoI CTYRAG 6 cut(s) 80, 108, 128, 140, 212, 224
SpeI ACTAGT 1 cut(s) 758
Sse9I AATT 4 cut(s) 29, 360, 416, 768
SsiI CCGC 1 cut(s) 57
SspMI CTAG 3 cut(s) 179, 716, 759
SstI GAGCTC 1 cut(s) 564
StyD4I CCNGG 2 cut(s) 7, 183
StyI CCWWGG 1 cut(s) 699
TaiI ACGT 2 cut(s) 235, 743
TaqI TCGA 2 cut(s) 241, 323
TasI AATT 4 cut(s) 29, 360, 416, 768
TatI WGTACW 1 cut(s) 603
TscAI CASTG 1 cut(s) 459
TspDTI ATGAA 3 cut(s) 8, 207, 738
TspRI CASTG 1 cut(s) 459
Vha464I CTTAAG 1 cut(s) 212
VpaK11BI GGWCC 1 cut(s) 49
XbaI TCTAGA 1 cut(s) 715
XspI CTAG 3 cut(s) 179, 716, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.