Rorug05G0369200

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002544
Physical Location & Seq
Reverse (-)
49433996 .. 49434886
891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0369200.1

Sequence Viewer

Length: 891 bp
ATGGCCTCCATGGATATGGAGATTCTTAGTTGGAATTGCAAAGGAATCTGTAACGATAATACTAGGAGGGCTTTGAAGGATCTCATCTCTCAAAACCGCCCTCAAATTGTTTTCCTTTGTGAAACCAAAATTAGCCTTTTATCTGATTTCAAGGCTCTTCATGCAACATTAGGGTTCCCTCATTCTCATGAGGTGCTAAGTGACGGTCAATCTGGTGGTTTGGGAATGTTTTGGAGTGAGGATGTGACGGCCACGATTGGAACTGTTTCTGCACACCATATGGATTTGTTTATTGATTGCGGGGTTGATAACCCTAGATGGAGATTGACGGGTTTCTATGGCTATGCTCGCACGGTCGAGCGAGATCGATCATGGCAATTGTTACGGGAGTTAAGCGACTTGGATACCTTGCCATGGGTCGTCATAGGGGATTTTAATGAAATTCTCAATAGCAGTGAAAAGAAGGACGGTCCAGTTCGAGAGGAGAGGCAGATGAGAGGCTTCCGTGATGCCCTAGGGTATGGAGACTTGCTGGATCTAAGGTTTCAGGGACCTCAATCTACGTGGTGGAATTCAGAGACACAACTCCGTTTAGATAGAGCGGTGTGCACGCCGACTTGGTGTGATATTTATGGCCATGCTCGTTTGCGGCACCTGCCACCTAGTGACTCTGATCATGTCCCAATCTTGTTGCATGCTAGCACGGTGCCTCTGGTAACGAGGACAAGGCATCATCGTTTCTGGTTCAAAGCGTACTGGTTACAGGATACTGAATGTGATGGCATTGTGGAGACAGAATGGGCTAGGGATATTTCTGGATCACCCATGTACCAAGTAACACTACACCAAAAACTGCATCACACGACGGTGAAAAAACGTCGTCTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

296

Amino Acids

34.18

Weight (kDa)

6.39

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 9 - 223 1.8e-20 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 663
Acc36I ACCTGC 1 cut(s) 663
AccB1I GGYRCC 2 cut(s) 651, 706
AccBSI CCGCTC 1 cut(s) 602
AciI CCGC 4 cut(s) 97, 300, 602, 649
AclWI GGATC 3 cut(s) 87, 543, 826
AcoI YGGCCR 2 cut(s) 249, 634
AcsI RAATTY 2 cut(s) 441, 571
AdeI CACNNNGTG 1 cut(s) 665
AfaI GTAC 2 cut(s) 755, 830
AfiI CCNNNNNNNGG 1 cut(s) 414
AgsI TTSAA 3 cut(s) 76, 151, 748
AleI CACNNNNGTG 1 cut(s) 866
Alw21I GWGCWC 1 cut(s) 611
Alw26I GTCTC 3 cut(s) 519, 572, 785
Alw44I GTGCAC 1 cut(s) 607
AlwI GGATC 3 cut(s) 87, 543, 826
AoxI GGCC 3 cut(s) 3, 249, 634
ApaLI GTGCAC 1 cut(s) 607
ApoI RAATTY 2 cut(s) 441, 571
Asp700I GAANNNNTTC 1 cut(s) 265
AspA2I CCTAGG 1 cut(s) 514
AspS9I GGNCC 2 cut(s) 470, 551
AsuHPI GGTGA 2 cut(s) 813, 880
AsuNHI GCTAGC 1 cut(s) 698
AvaII GGWCC 2 cut(s) 470, 551
AvrII CCTAGG 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 611
BaeI ACNNNNGTAYC 2 cut(s) 812, 845
BalI TGGCCA 1 cut(s) 636
BanI GGYRCC 2 cut(s) 651, 706
Bbv12I GWGCWC 1 cut(s) 611
BccI CCATC 2 cut(s) 312, 773
BceAI ACGGC 1 cut(s) 264
BciVI GTATCC 2 cut(s) 397, 760
BclI TGATCA 1 cut(s) 673
BcoDI GTCTC 3 cut(s) 519, 572, 785
BfaI CTAG 6 cut(s) 63, 315, 515, 663, 699, 804
BfuAI ACCTGC 1 cut(s) 663
BfuI GTATCC 2 cut(s) 397, 760
BisI GCNGC 1 cut(s) 650
BlnI CCTAGG 1 cut(s) 514
BlsI GCNGC 1 cut(s) 651
Bme18I GGWCC 2 cut(s) 470, 551
BmgT120I GGNCC 2 cut(s) 470, 551
BmiI GGNNCC 4 cut(s) 176, 552, 653, 708
BmsI GCATC 3 cut(s) 499, 739, 865
BmtI GCTAGC 1 cut(s) 702
Bsa29I ATCGAT 1 cut(s) 367
BsaAI YACGTR 1 cut(s) 564
BsaJI CCNNGG 3 cut(s) 9, 413, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 414
Bse1I ACTGG 2 cut(s) 473, 761
BseCI ATCGAT 1 cut(s) 367
BseDI CCNNGG 3 cut(s) 9, 413, 514
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 414
BseNI ACTGG 2 cut(s) 473, 761
BseRI GAGGAG 1 cut(s) 497
BseSI GKGCMC 1 cut(s) 611
BsgI GTGCAG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 3 cut(s) 5, 251, 636
BshNI GGYRCC 2 cut(s) 651, 706
BshVI ATCGAT 1 cut(s) 367
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 611
BslFI GGGAC 2 cut(s) 564, 665
BslI CCNNNNNNNGG 1 cut(s) 414
BsmAI GTCTC 3 cut(s) 519, 572, 785
BsmFI GGGAC 2 cut(s) 564, 665
BsnI GGCC 3 cut(s) 5, 251, 636
Bsp1286I GDGCHC 1 cut(s) 611
Bsp143I GATC 6 cut(s) 79, 364, 368, 535, 673, 818
Bsp19I CCATGG 2 cut(s) 9, 413
BspACI CCGC 4 cut(s) 97, 300, 602, 649
BspANI GGCC 3 cut(s) 5, 251, 636
BspDI ATCGAT 1 cut(s) 367
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 4 cut(s) 176, 552, 653, 708
BspMI ACCTGC 1 cut(s) 663
BspOI GCTAGC 1 cut(s) 702
BspPI GGATC 3 cut(s) 87, 543, 826
BspQI GCTCTTC 1 cut(s) 162
BspT107I GGYRCC 2 cut(s) 651, 706
BsrBI CCGCTC 1 cut(s) 602
BsrI ACTGG 2 cut(s) 473, 761
BssECI CCNNGG 3 cut(s) 9, 413, 514
BssMI GATC 6 cut(s) 79, 364, 368, 535, 673, 818
BssT1I CCWWGG 3 cut(s) 9, 413, 514
Bst4CI ACNGT 6 cut(s) 206, 265, 355, 470, 706, 868
Bst6I CTCTTC 1 cut(s) 162
BstBAI YACGTR 1 cut(s) 564
BstC8I GCNNGC 4 cut(s) 349, 611, 696, 700
BstDEI CTNAG 3 cut(s) 26, 197, 539
BstDSI CCRYGG 2 cut(s) 9, 413
BstF5I GGATG 1 cut(s) 247
BstKTI GATC 6 cut(s) 82, 367, 371, 538, 676, 821
BstMAI GTCTC 3 cut(s) 519, 572, 785
BstMBI GATC 6 cut(s) 79, 364, 368, 535, 673, 818
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 3 cut(s) 161, 348, 655
BstNSI RCATGY 1 cut(s) 698
BstSLI GKGCMC 1 cut(s) 611
BstX2I RGATCY 2 cut(s) 79, 535
BstXI CCANNNNNNTGG 1 cut(s) 16
BstYI RGATCY 2 cut(s) 79, 535
Bsu15I ATCGAT 1 cut(s) 367
BsuI GTATCC 2 cut(s) 397, 760
BsuRI GGCC 3 cut(s) 5, 251, 636
BsuTUI ATCGAT 1 cut(s) 367
BtgI CCRYGG 2 cut(s) 9, 413
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 460
BtsIMutI CAGTG 1 cut(s) 460
BveI ACCTGC 1 cut(s) 663
Cac8I GCNNGC 4 cut(s) 349, 611, 696, 700
CciI TCATGA 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 470, 551
ClaI ATCGAT 1 cut(s) 367
Csp6I GTAC 2 cut(s) 754, 829
CspCI CAANNNNNGTGG 2 cut(s) 545, 580
CviAII CATG 9 cut(s) 10, 161, 188, 372, 414, 638, 677, 695, 826
CviJI RGCY 9 cut(s) 5, 71, 135, 155, 251, 342, 501, 636, 803
CviKI_1 RGCY 9 cut(s) 5, 71, 135, 155, 251, 342, 501, 636, 803
CviQI GTAC 2 cut(s) 754, 829
DdeI CTNAG 3 cut(s) 26, 197, 539
DpnI GATC 6 cut(s) 81, 366, 370, 537, 675, 820
DpnII GATC 6 cut(s) 79, 364, 368, 535, 673, 818
DraIII CACNNNGTG 1 cut(s) 665
EaeI YGGCCR 2 cut(s) 249, 634
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Eco130I CCWWGG 3 cut(s) 9, 413, 514
Eco47I GGWCC 2 cut(s) 470, 551
EcoO109I RGGNCCY 1 cut(s) 551
EcoRI GAATTC 1 cut(s) 571
EcoT14I CCWWGG 3 cut(s) 9, 413, 514
ErhI CCWWGG 3 cut(s) 9, 413, 514
FaeI CATG 9 cut(s) 13, 164, 191, 375, 417, 641, 680, 698, 829
FaqI GGGAC 2 cut(s) 564, 665
FatI CATG 9 cut(s) 9, 160, 187, 371, 413, 637, 676, 694, 825
FauI CCCGC 1 cut(s) 293
FauNDI CATATG 1 cut(s) 279
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 1 cut(s) 650
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 1 cut(s) 650
FspBI CTAG 6 cut(s) 63, 315, 515, 663, 699, 804
GluI GCNGC 1 cut(s) 650
HaeIII GGCC 3 cut(s) 5, 251, 636
Hin1II CATG 9 cut(s) 13, 164, 191, 375, 417, 641, 680, 698, 829
HinfI GANTC 3 cut(s) 22, 45, 668
HphI GGTGA 2 cut(s) 813, 880
Hpy166II GTNNAC 1 cut(s) 609
Hpy188I TCNGA 4 cut(s) 145, 577, 673, 885
Hpy188III TCNNGA 3 cut(s) 188, 479, 816
Hpy8I GTNNAC 1 cut(s) 609
Hpy99I CGWCG 2 cut(s) 868, 882
HpyAV CCTTC 2 cut(s) 70, 457
HpyCH4III ACNGT 6 cut(s) 206, 265, 355, 470, 706, 868
HpyCH4IV ACGT 2 cut(s) 563, 877
HpyCH4V TGCA 6 cut(s) 39, 164, 272, 609, 694, 856
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 348, 655
HpyF3I CTNAG 3 cut(s) 26, 197, 539
HpySE526I ACGT 2 cut(s) 563, 877
Hsp92II CATG 9 cut(s) 13, 164, 191, 375, 417, 641, 680, 698, 829
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 6 cut(s) 79, 364, 368, 535, 673, 818
LguI GCTCTTC 1 cut(s) 162
LweI GCATC 3 cut(s) 499, 739, 865
MaeI CTAG 6 cut(s) 63, 315, 515, 663, 699, 804
MaeII ACGT 2 cut(s) 563, 877
MaeIII GTNAC 8 cut(s) 50, 200, 244, 381, 665, 715, 759, 835
MalI GATC 6 cut(s) 81, 366, 370, 537, 675, 820
MbiI CCGCTC 1 cut(s) 602
MboI GATC 6 cut(s) 79, 364, 368, 535, 673, 818
MboII GAAGA 1 cut(s) 149
MfeI CAATTG 1 cut(s) 377
MflI RGATCY 2 cut(s) 79, 535
MhlI GDGCHC 1 cut(s) 611
MlsI TGGCCA 1 cut(s) 636
MluCI AATT 6 cut(s) 34, 105, 129, 377, 441, 571
MluNI TGGCCA 1 cut(s) 636
MlyI GAGTC 1 cut(s) 662
MmeI TCCRAC 1 cut(s) 11
Mox20I TGGCCA 1 cut(s) 636
MroXI GAANNNNTTC 1 cut(s) 265
MscI TGGCCA 1 cut(s) 636
MseI TTAA 2 cut(s) 392, 435
MslI CAYNNNNRTG 3 cut(s) 14, 186, 866
Msp20I TGGCCA 1 cut(s) 636
MunI CAATTG 1 cut(s) 377
MwoI GCNNNNNNNGC 3 cut(s) 161, 348, 655
NcoI CCATGG 2 cut(s) 9, 413
NdeI CATATG 1 cut(s) 279
NdeII GATC 6 cut(s) 79, 364, 368, 535, 673, 818
NheI GCTAGC 1 cut(s) 698
NlaIII CATG 9 cut(s) 13, 164, 191, 375, 417, 641, 680, 698, 829
NlaIV GGNNCC 4 cut(s) 176, 552, 653, 708
NmuCI GTSAC 3 cut(s) 200, 244, 665
NspI RCATGY 1 cut(s) 698
OliI CACNNNNGTG 1 cut(s) 866
PaeI GCATGC 1 cut(s) 698
PagI TCATGA 1 cut(s) 187
PaqCI CACCTGC 1 cut(s) 663
PciSI GCTCTTC 1 cut(s) 162
PdmI GAANNNNTTC 1 cut(s) 265
PfeI GAWTC 2 cut(s) 22, 45
PkrI GCNGC 1 cut(s) 651
PleI GAGTC 1 cut(s) 662
PpsI GAGTC 1 cut(s) 662
Ppu21I YACGTR 1 cut(s) 564
PpuMI RGGWCCY 1 cut(s) 551
Psp5II RGGWCCY 1 cut(s) 551
PspN4I GGNNCC 4 cut(s) 176, 552, 653, 708
PspPI GGNCC 2 cut(s) 470, 551
PspPPI RGGWCCY 1 cut(s) 551
PsuI RGATCY 2 cut(s) 79, 535
RsaI GTAC 2 cut(s) 755, 830
RsaNI GTAC 2 cut(s) 754, 829
RseI CAYNNNNRTG 3 cut(s) 14, 186, 866
SapI GCTCTTC 1 cut(s) 162
SaqAI TTAA 2 cut(s) 392, 435
SatI GCNGC 1 cut(s) 650
Sau3AI GATC 6 cut(s) 79, 364, 368, 535, 673, 818
Sau96I GGNCC 2 cut(s) 470, 551
SchI GAGTC 1 cut(s) 662
SduI GDGCHC 1 cut(s) 611
SetI ASST 8 cut(s) 195, 410, 545, 556, 566, 657, 664, 880
SfaNI GCATC 3 cut(s) 499, 739, 865
SinI GGWCC 2 cut(s) 470, 551
SmiMI CAYNNNNRTG 3 cut(s) 14, 186, 866
SphI GCATGC 1 cut(s) 698
Sse9I AATT 6 cut(s) 34, 105, 129, 377, 441, 571
SsiI CCGC 4 cut(s) 97, 300, 602, 649
SspMI CTAG 6 cut(s) 63, 315, 515, 663, 699, 804
StyI CCWWGG 3 cut(s) 9, 413, 514
TaaI ACNGT 6 cut(s) 206, 265, 355, 470, 706, 868
TaiI ACGT 2 cut(s) 566, 880
TaqI TCGA 3 cut(s) 357, 367, 478
TasI AATT 6 cut(s) 34, 105, 129, 377, 441, 571
TauI GCSGC 1 cut(s) 652
TfiI GAWTC 2 cut(s) 22, 45
Tru1I TTAA 2 cut(s) 392, 435
Tru9I TTAA 2 cut(s) 392, 435
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 3 cut(s) 200, 244, 665
Tsp45I GTSAC 3 cut(s) 200, 244, 665
TspDTI ATGAA 2 cut(s) 149, 453
TspGWI ACGGA 2 cut(s) 494, 578
TspRI CASTG 1 cut(s) 460
VneI GTGCAC 1 cut(s) 607
VpaK11BI GGWCC 2 cut(s) 470, 551
XapI RAATTY 2 cut(s) 441, 571
XceI RCATGY 1 cut(s) 698
XmaJI CCTAGG 1 cut(s) 514
XmnI GAANNNNTTC 1 cut(s) 265
XspI CTAG 6 cut(s) 63, 315, 515, 663, 699, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.