Rh5DG455500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002544
Physical Location & Seq
Reverse (-)
72522446 .. 72522844
399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG455500.1

Sequence Viewer

Length: 399 bp
ATGGCCTTGAACGAACTGAAGATCTTGGGAGCACAGCTAAATGGGGTCCGAAAGGACTTGTCCAAGTCCCGGGCTAAGTTGGAATTTGGTAAGAAGGTGTGGGAGACTGAGAAAATGGAACTGACTAAGAAGCTTAACAAGTTTGGAACAGAGATAAAGGAGATCAGAGAGGAGTTGTCTGACTTGGCCAAGATCCACGAGAAGCACATTCAAGAAAATGAGAATGCATATTGCGCAGGGTACCTGACTGGATGGTTCAGGGAGCCCCATGAATATGTTTCCCTGCGAAGCCATGAGGCCAAGGAAGTTACTCTTCCGGTTCATAAACCTCTGGAGGCCACATCTTCACTTTCTCATTCTCGTGCCTTAATTAGACGTTTCGGAAGTGCTGGTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

15.1

Weight (kDa)

9.19

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 235
Acc65I GGTACC 1 cut(s) 240
AccB1I GGYRCC 1 cut(s) 240
AclWI GGATC 1 cut(s) 187
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 1 cut(s) 83
AcuI CTGAAG 1 cut(s) 38
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 2 cut(s) 10, 212
AluBI AGCT 2 cut(s) 37, 133
AluI AGCT 2 cut(s) 37, 133
Alw21I GWGCWC 1 cut(s) 34
Alw26I GTCTC 1 cut(s) 98
AlwI GGATC 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 69
AoxI GGCC 4 cut(s) 3, 186, 297, 336
ApoI RAATTY 1 cut(s) 83
Asp718I GGTACC 1 cut(s) 240
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 2 cut(s) 46, 392
AsuC2I CCSGG 2 cut(s) 70, 71
AvaI CYCGRG 1 cut(s) 69
AvaII GGWCC 2 cut(s) 46, 392
BalI TGGCCA 1 cut(s) 188
BanI GGYRCC 1 cut(s) 240
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 2 cut(s) 197, 360
Bbv12I GWGCWC 1 cut(s) 34
BccI CCATC 1 cut(s) 246
BcnI CCSGG 2 cut(s) 70, 71
BcoDI GTCTC 1 cut(s) 98
BglII AGATCT 1 cut(s) 21
Bme1390I CCNGG 2 cut(s) 70, 71
Bme18I GGWCC 2 cut(s) 46, 392
BmeT110I CYCGRG 1 cut(s) 69
BmgT120I GGNCC 2 cut(s) 46, 392
BmiI GGNNCC 3 cut(s) 47, 242, 264
BmrFI CCNGG 2 cut(s) 70, 71
BpmI CTGGAG 1 cut(s) 353
BpuMI CCSGG 2 cut(s) 70, 71
BsaJI CCNNGG 2 cut(s) 69, 300
BsaWI WCCGGW 1 cut(s) 316
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse1I ACTGG 1 cut(s) 253
BseDI CCNNGG 2 cut(s) 69, 300
BseGI GGATG 1 cut(s) 257
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 99
BseNI ACTGG 1 cut(s) 253
BseRI GAGGAG 1 cut(s) 185
BshFI GGCC 4 cut(s) 5, 188, 299, 338
BshNI GGYRCC 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 34
BsiHKCI CYCGRG 1 cut(s) 69
BsiSI CCGG 2 cut(s) 70, 317
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 69
BsmAI GTCTC 1 cut(s) 98
BsmFI GGGAC 1 cut(s) 52
BsmI GAATGC 1 cut(s) 229
BsnI GGCC 4 cut(s) 5, 188, 299, 338
BsoBI CYCGRG 1 cut(s) 69
Bsp1286I GDGCHC 2 cut(s) 34, 267
Bsp143I GATC 3 cut(s) 21, 162, 192
BspANI GGCC 4 cut(s) 5, 188, 299, 338
BspCNI CTCAG 1 cut(s) 100
BspLI GGNNCC 3 cut(s) 47, 242, 264
BspPI GGATC 1 cut(s) 187
BspT107I GGYRCC 1 cut(s) 240
BsrI ACTGG 1 cut(s) 253
BssECI CCNNGG 2 cut(s) 69, 300
BssMI GATC 3 cut(s) 21, 162, 192
BssSI CACGAG 2 cut(s) 197, 360
BssT1I CCWWGG 1 cut(s) 300
Bst2BI CACGAG 2 cut(s) 197, 360
Bst6I CTCTTC 1 cut(s) 318
BstDEI CTNAG 3 cut(s) 75, 108, 126
BstF5I GGATG 1 cut(s) 257
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 3 cut(s) 24, 165, 195
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 3 cut(s) 21, 162, 192
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstSCI CCNGG 2 cut(s) 68, 69
BstX2I RGATCY 2 cut(s) 21, 192
BstYI RGATCY 2 cut(s) 21, 192
BsuRI GGCC 4 cut(s) 5, 188, 299, 338
BtsCI GGATG 1 cut(s) 257
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 2 cut(s) 46, 392
Cfr9I CCCGGG 1 cut(s) 69
Csp6I GTAC 1 cut(s) 241
CviAII CATG 2 cut(s) 269, 293
CviJI RGCY 9 cut(s) 5, 37, 74, 133, 188, 265, 291, 299, 338
CviKI_1 RGCY 9 cut(s) 5, 37, 74, 133, 188, 265, 291, 299, 338
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 3 cut(s) 75, 108, 126
DpnI GATC 3 cut(s) 23, 164, 194
DpnII GATC 3 cut(s) 21, 162, 192
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 2 cut(s) 46, 392
Eco57I CTGAAG 1 cut(s) 38
Eco88I CYCGRG 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 300
EcoT22I ATGCAT 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 2 cut(s) 272, 296
FaiI YATR 6 cut(s) 229, 270, 276, 294, 324, 397
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FaqI GGGAC 1 cut(s) 52
FatI CATG 2 cut(s) 268, 292
FokI GGATG 1 cut(s) 264
FriOI GRGCYC 1 cut(s) 267
FspI TGCGCA 1 cut(s) 235
GlaI GCGC 1 cut(s) 235
GsuI CTGGAG 1 cut(s) 353
HaeIII GGCC 4 cut(s) 5, 188, 299, 338
HapII CCGG 2 cut(s) 70, 317
HhaI GCGC 1 cut(s) 236
Hin1II CATG 2 cut(s) 272, 296
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 131
HpaII CCGG 2 cut(s) 70, 317
Hpy188I TCNGA 4 cut(s) 50, 167, 181, 383
Hpy188III TCNNGA 2 cut(s) 212, 332
HpyAV CCTTC 1 cut(s) 88
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 1 cut(s) 227
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 3 cut(s) 75, 108, 126
HpySE526I ACGT 1 cut(s) 376
Hsp92II CATG 2 cut(s) 272, 296
HspAI GCGC 1 cut(s) 234
KpnI GGTACC 1 cut(s) 244
Kzo9I GATC 3 cut(s) 21, 162, 192
LmnI GCTCC 2 cut(s) 29, 262
LpnPI CCDG 9 cut(s) 83, 222, 234, 244, 257, 296, 317, 330, 375
MaeII ACGT 1 cut(s) 376
MaeIII GTNAC 1 cut(s) 307
MalI GATC 3 cut(s) 23, 164, 194
MboI GATC 3 cut(s) 21, 162, 192
MboII GAAGA 3 cut(s) 31, 305, 336
MflI RGATCY 2 cut(s) 21, 192
MhlI GDGCHC 2 cut(s) 34, 267
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 2 cut(s) 83, 369
MluNI TGGCCA 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 4 cut(s) 163, 289, 328, 339
Mox20I TGGCCA 1 cut(s) 188
Mph1103I ATGCAT 1 cut(s) 229
MscI TGGCCA 1 cut(s) 188
MseI TTAA 2 cut(s) 135, 368
MslI CAYNNNNRTG 2 cut(s) 273, 360
Msp20I TGGCCA 1 cut(s) 188
MspI CCGG 2 cut(s) 70, 317
MspR9I CCNGG 2 cut(s) 70, 71
Mva1269I GAATGC 1 cut(s) 229
MwoI GCNNNNNNNGC 1 cut(s) 233
NciI CCSGG 2 cut(s) 70, 71
NdeII GATC 3 cut(s) 21, 162, 192
NlaIII CATG 2 cut(s) 272, 296
NlaIV GGNNCC 3 cut(s) 47, 242, 264
NsbI TGCGCA 1 cut(s) 235
NsiI ATGCAT 1 cut(s) 229
PctI GAATGC 1 cut(s) 229
PspN4I GGNNCC 3 cut(s) 47, 242, 264
PspPI GGNCC 2 cut(s) 46, 392
PsuI RGATCY 2 cut(s) 21, 192
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 2 cut(s) 273, 360
SaqAI TTAA 2 cut(s) 135, 368
Sau3AI GATC 3 cut(s) 21, 162, 192
Sau96I GGNCC 2 cut(s) 46, 392
ScrFI CCNGG 2 cut(s) 70, 71
SduI GDGCHC 2 cut(s) 34, 267
SetI ASST 6 cut(s) 39, 99, 135, 246, 331, 379
SinI GGWCC 2 cut(s) 46, 392
SmaI CCCGGG 1 cut(s) 71
SmiMI CAYNNNNRTG 2 cut(s) 273, 360
Sse9I AATT 2 cut(s) 83, 369
StyD4I CCNGG 2 cut(s) 68, 69
StyI CCWWGG 1 cut(s) 300
TaiI ACGT 1 cut(s) 379
TasI AATT 2 cut(s) 83, 369
Tru1I TTAA 2 cut(s) 135, 368
Tru9I TTAA 2 cut(s) 135, 368
TspDTI ATGAA 2 cut(s) 285, 311
TspMI CCCGGG 1 cut(s) 69
VpaK11BI GGWCC 2 cut(s) 46, 392
XapI RAATTY 1 cut(s) 83
XmaI CCCGGG 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.