Rmu_sc0005450.1_g000014

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005450.1
OG0002544
Physical Location & Seq
Forward (+)
52659 .. 56702
4044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005450.1_g000014.1.cds

Sequence Viewer

Length: 1356 bp
atgtgggttatgaaggattatggtatccaagagtcttggactagaagtgttgttattgaaaacttctttccaatggaacttagctttgattgttatgaacctattatcctagccttgagcaatggagaaattctgatgttcaatgatgatgatcctcgggtcgtagtttgttataatccaggagcaaagagtttcagggaagctagaattactacgactaccttgccgttgattcaacttgcttaccgtccatcctttgtttcgctctatgatgtttcaaaaagagaggaggtgaaaaggtttgctgcaatggttccaggagaacttcatcttgggaaattgcatcaagatttactggacctgcgcggtcattttgagaagttagtccttgagcttcaaaaagctctgttaaaatctcaagagcaaaagaaagatcttgagaaacaacttgacgagagcgaaaataaaagggaagagcttaacaagctagtcctggactatgaaggtaatatcaaggatcttaaggatgatcttgagcaacgtgaagctcaaacaaaggatcctaagaatagtgttgatcagagtggagatggcattaagaaagaacttgatcaggttagcttttatgagtcgaggattgaaaatatgaccaaagagcgtgatggcttaattaacaagttgaaaagcgtaggagcagagctaaaggaagcccaagaggacttggaattaggcaagaaggtttggaaggttgagaggatagcactggctgagaggcttactaatgcaaaggaggtgtgggaggctgagaagatagcactgacgaatgaaattctcgcaaaagaatgccaggactccacttgcaaatacaaccaagctttgaaggagcgagacgcagccttgagtgatctgaataacctgggaatagagctagaggaggttcgaaaggtcttgtccgagtcccaggctaactcggaattaggcaagaaggtttgggaggctgagagcatagcactggctgagaggcttactaatgcaaaggaggtgtgggaggctgagaagatggaactgactaaaaagcttaaccatgagttaaacaagtttggaatagagataaaggagctcagagaggagttgtctgacttggagaagatccacaagcagtacattcaagaaaatgagaacgcatattgctcagggtacctgactggttggttcaaggagcctcatgcctacatttacctgccaagccatggggccaaggaagttactcttctagatcatgaacctctggaggccacgtctccactctctcacactagtgccttaattaaacttttcggaagtgctgatccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

451

Amino Acids

51.82

Weight (kDa)

5.03

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 174
Acc36I ACCTGC 2 cut(s) 369, 1248
Acc65I GGTACC 1 cut(s) 1197
AccB1I GGYRCC 1 cut(s) 1197
AccB7I CCANNNNNTGG 1 cut(s) 1250
AccII CGCG 1 cut(s) 366
AciI CCGC 1 cut(s) 366
AclWI GGATC 6 cut(s) 146, 525, 554, 567, 1144, 1343
AcsI RAATTY 2 cut(s) 129, 828
AfaI GTAC 2 cut(s) 1163, 1199
AfiI CCNNNNNNNGG 1 cut(s) 1250
AflII CTTAAG 1 cut(s) 521
AhlI ACTAGT 1 cut(s) 1316
AjiI CACGTC 1 cut(s) 1299
AjnI CCWGG 6 cut(s) 178, 316, 492, 846, 915, 960
AjuI GAANNNNNNNTTGG 2 cut(s) 315, 347
AleI CACNNNNGTG 1 cut(s) 1317
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
Alw21I GWGCWC 1 cut(s) 1122
Alw26I GTCTC 2 cut(s) 882, 1305
AlwI GGATC 6 cut(s) 146, 525, 554, 567, 1144, 1343
Ama87I CYCGRG 1 cut(s) 156
AoxI GGCC 2 cut(s) 1254, 1293
ApeKI GCWGC 2 cut(s) 305, 893
ApoI RAATTY 2 cut(s) 129, 828
Asp718I GGTACC 1 cut(s) 1197
AspLEI GCGC 1 cut(s) 366
AspS9I GGNCC 2 cut(s) 358, 1254
AsuHPI GGTGA 1 cut(s) 304
AsuII TTCGAA 1 cut(s) 940
AvaI CYCGRG 1 cut(s) 156
AvaII GGWCC 1 cut(s) 358
BamHI GGATCC 1 cut(s) 559
BanI GGYRCC 1 cut(s) 1197
BanII GRGCYC 1 cut(s) 1122
Bbv12I GWGCWC 1 cut(s) 1122
BbvI GCAGC 2 cut(s) 292, 905
BccI CCATC 4 cut(s) 259, 584, 656, 1054
BceAI ACGGC 1 cut(s) 211
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 6 cut(s) 180, 318, 494, 848, 917, 962
BciVI GTATCC 1 cut(s) 35
BclI TGATCA 2 cut(s) 577, 610
BcoDI GTCTC 2 cut(s) 882, 1305
BcuI ACTAGT 1 cut(s) 1316
BfaI CTAG 7 cut(s) 42, 110, 204, 488, 929, 1274, 1317
BfrI CTTAAG 1 cut(s) 521
BfuAI ACCTGC 2 cut(s) 369, 1248
BfuI GTATCC 1 cut(s) 35
BglII AGATCT 1 cut(s) 433
BisI GCNGC 2 cut(s) 306, 894
BlsI GCNGC 2 cut(s) 307, 895
Bme1390I CCNGG 6 cut(s) 180, 318, 494, 848, 917, 962
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 156
BmgBI CACGTC 1 cut(s) 1299
BmgT120I GGNCC 2 cut(s) 358, 1254
BmiI GGNNCC 5 cut(s) 315, 561, 1199, 1221, 1255
BmrFI CCNGG 6 cut(s) 180, 318, 494, 848, 917, 962
BmsI GCATC 1 cut(s) 352
BpmI CTGGAG 1 cut(s) 1310
Bpu10I CCTNAGC 1 cut(s) 1192
Bpu14I TTCGAA 1 cut(s) 940
BpuEI CTTGAG 6 cut(s) 136, 402, 410, 458, 554, 919
BsaBI GATNNNNATC 1 cut(s) 150
BsaJI CCNNGG 5 cut(s) 155, 916, 960, 1249, 1257
BsaXI ACNNNNNCTCC 2 cut(s) 1283, 1313
Bsc4I CCNNNNNNNGG 1 cut(s) 1250
Bse1I ACTGG 4 cut(s) 360, 768, 1017, 1210
Bse3DI GCAATG 2 cut(s) 127, 315
Bse8I GATNNNNATC 1 cut(s) 150
BseBI CCWGG 6 cut(s) 180, 318, 494, 848, 917, 962
BseDI CCNNGG 5 cut(s) 155, 916, 960, 1249, 1257
BseGI GGATG 2 cut(s) 251, 532
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 1250
BseMI GCAATG 2 cut(s) 127, 315
BseMII CTCAG 7 cut(s) 759, 795, 990, 1008, 1044, 1135, 1206
BseNI ACTGG 4 cut(s) 360, 768, 1017, 1210
BseRI GAGGAG 3 cut(s) 302, 947, 1142
BseXI GCAGC 2 cut(s) 292, 905
Bsh1236I CGCG 1 cut(s) 366
BshFI GGCC 2 cut(s) 1256, 1295
BshNI GGYRCC 1 cut(s) 1197
BsiHKAI GWGCWC 1 cut(s) 1122
BsiHKCI CYCGRG 1 cut(s) 156
BslFI GGGAC 1 cut(s) 943
BslI CCNNNNNNNGG 1 cut(s) 1250
BsmAI GTCTC 2 cut(s) 882, 1305
BsmBI CGTCTC 2 cut(s) 882, 1305
BsmFI GGGAC 1 cut(s) 943
BsmI GAATGC 1 cut(s) 848
BsnI GGCC 2 cut(s) 1256, 1295
BsoBI CYCGRG 1 cut(s) 156
Bsp119I TTCGAA 1 cut(s) 940
Bsp1286I GDGCHC 1 cut(s) 1122
Bsp19I CCATGG 1 cut(s) 1249
BspACI CCGC 1 cut(s) 366
BspANI GGCC 2 cut(s) 1256, 1295
BspCNI CTCAG 7 cut(s) 760, 796, 991, 1009, 1045, 1134, 1205
BspFNI CGCG 1 cut(s) 366
BspHI TCATGA 1 cut(s) 1279
BspLI GGNNCC 5 cut(s) 315, 561, 1199, 1221, 1255
BspMI ACCTGC 2 cut(s) 369, 1248
BspPI GGATC 6 cut(s) 146, 525, 554, 567, 1144, 1343
BspQI GCTCTTC 1 cut(s) 468
BspT104I TTCGAA 1 cut(s) 940
BspT107I GGYRCC 1 cut(s) 1197
BspTI CTTAAG 1 cut(s) 521
BsrDI GCAATG 2 cut(s) 127, 315
BsrI ACTGG 4 cut(s) 360, 768, 1017, 1210
BssECI CCNNGG 5 cut(s) 155, 916, 960, 1249, 1257
BssT1I CCWWGG 2 cut(s) 1249, 1257
Bst2UI CCWGG 6 cut(s) 180, 318, 494, 848, 917, 962
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 2 cut(s) 468, 1275
BstAFI CTTAAG 1 cut(s) 521
BstBI TTCGAA 1 cut(s) 940
BstDEI CTNAG 9 cut(s) 80, 564, 768, 804, 999, 1017, 1053, 1121, 1192
BstDSI CCRYGG 1 cut(s) 1249
BstF5I GGATG 2 cut(s) 251, 532
BstFNI CGCG 1 cut(s) 366
BstHHI GCGC 1 cut(s) 366
BstMAI GTCTC 2 cut(s) 882, 1305
BstMWI GCNNNNNNNGC 1 cut(s) 484
BstNI CCWGG 6 cut(s) 180, 318, 494, 848, 917, 962
BstSCI CCNGG 6 cut(s) 178, 316, 492, 846, 915, 960
BstUI CGCG 1 cut(s) 366
BstV1I GCAGC 2 cut(s) 292, 905
BstX2I RGATCY 4 cut(s) 433, 517, 559, 1149
BstYI RGATCY 4 cut(s) 433, 517, 559, 1149
BsuI GTATCC 1 cut(s) 35
BsuRI GGCC 2 cut(s) 1256, 1295
BtgI CCRYGG 1 cut(s) 1249
BtrI CACGTC 1 cut(s) 1299
BtsCI GGATG 2 cut(s) 251, 532
BtsIMutI CAGTG 3 cut(s) 761, 815, 1010
BveI ACCTGC 2 cut(s) 369, 1248
CciI TCATGA 1 cut(s) 1279
CfoI GCGC 1 cut(s) 366
Cfr13I GGNCC 2 cut(s) 358, 1254
CseI GACGC 1 cut(s) 899
Csp6I GTAC 2 cut(s) 1162, 1198
CviAII CATG 4 cut(s) 1085, 1226, 1250, 1280
CviQI GTAC 2 cut(s) 1162, 1198
DdeI CTNAG 9 cut(s) 80, 564, 768, 804, 999, 1017, 1053, 1121, 1192
Eam1104I CTCTTC 2 cut(s) 468, 1275
EarI CTCTTC 2 cut(s) 468, 1275
Ecl136II GAGCTC 1 cut(s) 1120
Eco130I CCWWGG 2 cut(s) 1249, 1257
Eco24I GRGCYC 1 cut(s) 1122
Eco47I GGWCC 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 1120
Eco88I CYCGRG 1 cut(s) 156
EcoICRI GAGCTC 1 cut(s) 1120
EcoRII CCWGG 6 cut(s) 178, 316, 492, 846, 915, 960
EcoT14I CCWWGG 2 cut(s) 1249, 1257
EcoT38I GRGCYC 1 cut(s) 1122
ErhI CCWWGG 2 cut(s) 1249, 1257
Esp3I CGTCTC 2 cut(s) 882, 1305
FaeI CATG 4 cut(s) 1088, 1229, 1253, 1283
FalI AAGNNNNNCTT 2 cut(s) 1254, 1286
FaqI GGGAC 1 cut(s) 943
FatI CATG 4 cut(s) 1084, 1225, 1249, 1279
FbaI TGATCA 2 cut(s) 577, 610
Fnu4HI GCNGC 2 cut(s) 306, 894
FokI GGATG 2 cut(s) 238, 539
FriOI GRGCYC 1 cut(s) 1122
Fsp4HI GCNGC 2 cut(s) 306, 894
FspBI CTAG 7 cut(s) 42, 110, 204, 488, 929, 1274, 1317
GlaI GCGC 1 cut(s) 365
GluI GCNGC 2 cut(s) 306, 894
GsuI CTGGAG 1 cut(s) 1310
HaeIII GGCC 2 cut(s) 1256, 1295
HgaI GACGC 1 cut(s) 899
HhaI GCGC 1 cut(s) 366
Hin1II CATG 4 cut(s) 1088, 1229, 1253, 1283
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HindIII AAGCTT 2 cut(s) 873, 1076
HinfI GANTC 5 cut(s) 32, 232, 629, 851, 956
HphI GGTGA 1 cut(s) 304
Hpy188I TCNGA 8 cut(s) 135, 582, 909, 955, 973, 1124, 1138, 1340
Hpy188III TCNNGA 8 cut(s) 347, 419, 437, 533, 1169, 1274, 1280, 1289
HpyAV CCTTC 6 cut(s) 7, 497, 730, 739, 874, 979
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4IV ACGT 2 cut(s) 541, 1298
HpyCH4V TGCA 5 cut(s) 308, 343, 785, 861, 1034
HpyF10VI GCNNNNNNNGC 1 cut(s) 484
HpyF3I CTNAG 9 cut(s) 80, 564, 768, 804, 999, 1017, 1053, 1121, 1192
HpySE526I ACGT 2 cut(s) 541, 1298
Hsp92II CATG 4 cut(s) 1088, 1229, 1253, 1283
HspAI GCGC 1 cut(s) 364
KpnI GGTACC 1 cut(s) 1201
Ksp22I TGATCA 2 cut(s) 577, 610
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 5 cut(s) 182, 692, 883, 1117, 1219
Lsp1109I GCAGC 2 cut(s) 292, 905
LweI GCATC 1 cut(s) 352
MaeI CTAG 7 cut(s) 42, 110, 204, 488, 929, 1274, 1317
MaeII ACGT 2 cut(s) 541, 1298
MaeIII GTNAC 1 cut(s) 1264
MboII GAAGA 5 cut(s) 485, 820, 1069, 1159, 1262
MflI RGATCY 4 cut(s) 433, 517, 559, 1149
MhlI GDGCHC 1 cut(s) 1122
MluCI AATT 8 cut(s) 129, 207, 338, 669, 725, 828, 974, 1326
MlyI GAGTC 4 cut(s) 41, 638, 845, 965
MslI CAYNNNNRTG 1 cut(s) 1317
MspCI CTTAAG 1 cut(s) 521
MspR9I CCNGG 6 cut(s) 180, 318, 494, 848, 917, 962
Mva1269I GAATGC 1 cut(s) 848
MvaI CCWGG 6 cut(s) 180, 318, 494, 848, 917, 962
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 1 cut(s) 484
NcoI CCATGG 1 cut(s) 1249
NlaIII CATG 4 cut(s) 1088, 1229, 1253, 1283
NlaIV GGNNCC 5 cut(s) 315, 561, 1199, 1221, 1255
NspV TTCGAA 1 cut(s) 940
OliI CACNNNNGTG 1 cut(s) 1317
PacI TTAATTAA 2 cut(s) 672, 1329
PagI TCATGA 1 cut(s) 1279
PciSI GCTCTTC 1 cut(s) 468
PctI GAATGC 1 cut(s) 848
PfeI GAWTC 1 cut(s) 232
PflMI CCANNNNNTGG 1 cut(s) 1250
PfoI TCCNGGA 3 cut(s) 178, 316, 492
PkrI GCNGC 2 cut(s) 307, 895
PleI GAGTC 4 cut(s) 40, 637, 845, 964
PpsI GAGTC 4 cut(s) 40, 637, 845, 964
PsiI TTATAA 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 1122
Psp6I CCWGG 6 cut(s) 178, 316, 492, 846, 915, 960
PspGI CCWGG 6 cut(s) 178, 316, 492, 846, 915, 960
PspN4I GGNNCC 5 cut(s) 315, 561, 1199, 1221, 1255
PspPI GGNCC 2 cut(s) 358, 1254
PsuI RGATCY 4 cut(s) 433, 517, 559, 1149
RsaI GTAC 2 cut(s) 1163, 1199
RsaNI GTAC 2 cut(s) 1162, 1198
RseI CAYNNNNRTG 1 cut(s) 1317
SacI GAGCTC 1 cut(s) 1122
SapI GCTCTTC 1 cut(s) 468
SatI GCNGC 2 cut(s) 306, 894
Sau96I GGNCC 2 cut(s) 358, 1254
SchI GAGTC 4 cut(s) 41, 638, 845, 965
ScrFI CCNGG 6 cut(s) 180, 318, 494, 848, 917, 962
SduI GDGCHC 1 cut(s) 1122
SfaNI GCATC 1 cut(s) 352
SfuI TTCGAA 1 cut(s) 940
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 1317
SmlI CTYRAG 7 cut(s) 115, 389, 417, 437, 521, 533, 898
SmoI CTYRAG 7 cut(s) 115, 389, 417, 437, 521, 533, 898
SpeI ACTAGT 1 cut(s) 1316
Sse9I AATT 8 cut(s) 129, 207, 338, 669, 725, 828, 974, 1326
SsiI CCGC 1 cut(s) 366
SspMI CTAG 7 cut(s) 42, 110, 204, 488, 929, 1274, 1317
SstI GAGCTC 1 cut(s) 1122
StyD4I CCNGG 6 cut(s) 178, 316, 492, 846, 915, 960
StyI CCWWGG 2 cut(s) 1249, 1257
TaaI ACNGT 1 cut(s) 248
TaiI ACGT 2 cut(s) 544, 1301
TaqI TCGA 2 cut(s) 632, 940
TasI AATT 8 cut(s) 129, 207, 338, 669, 725, 828, 974, 1326
TatI WGTACW 1 cut(s) 1161
TfiI GAWTC 1 cut(s) 232
TscAI CASTG 3 cut(s) 768, 822, 1017
TseI GCWGC 2 cut(s) 305, 893
TspDTI ATGAA 6 cut(s) 26, 111, 317, 516, 840, 1296
TspRI CASTG 3 cut(s) 768, 822, 1017
Van91I CCANNNNNTGG 1 cut(s) 1250
Vha464I CTTAAG 1 cut(s) 521
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 2 cut(s) 129, 828
XbaI TCTAGA 1 cut(s) 1273
XspI CTAG 7 cut(s) 42, 110, 204, 488, 929, 1274, 1317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.