Rh5BG442400

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002544
Physical Location & Seq
Reverse (-)
71024923 .. 71035410
10488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG442400.1

Sequence Viewer

Length: 1284 bp
ATGGAGCTCATAGAGGAGTTGTCTGACTTGGAGAAGATCCACAAGCAGCACATTCAAGAAAATGAGAACGCATATTGCTCAGGGTTCCTGATTGGTTGGTTCAAGGAACCTCATGCCTACATTTCCCTGCCAAGCCATGAGGCCAAGGACGTTGCTCTTCTAGATCATGAACCTCTGGAGGCCCCATCTCCACTCTCTCACACTTCTACCTTAATTAAACTTTTCGAAAGCAATTTTCATCAAGTTTTACTGAACCTACGTAGCCAGGTTGAGAAGATAGTCAATGAGGGGCAGAGAGCTCTGTTAGAATCTCCAGAGCAAAAGGAAGACCTAAGAAAACAGCTTGAGAAAAGTGAAGCTCAAAACAAAGATCTTGAGAAAGAACTTGAGAAGAGTGAGGCTCAAAATCAAGAGCTTCACAATATAGTCTTAGACCAGCATACAGCCTTGTCAAGCTATGAAGATCAAATCAAGAATCTAAAGAAAGAACTTGATCAGGCTGGCTCTCATGAGTCGAGGAGTGAAATTATGACCAAAGAGCGTGATGGCTTGATTAGCAAGTACAACCAAGCATTGAAGGAGCGAGACTCAGCCTTAGAGAAGTTGAATACCATTGGAGCAGAGCTAAAGGAGGCGCAAAAAGACTTGGAATTGAGCAAGAATGTCTGGGATGCTGAAAGGATAGCTTTGGCTGAGAGGCTTACTAAGGCGAAAAAGGCGTGGGAGGCTGAGAGGATGGCACTGACAAGTACAATTTTGACGCACCAGGACTCCACTTGGAAATACAACCAAGCTTTAAAGGAGCGAGACTCAGCCTTGAAGGAGGTCCAAAAAGATTTGTCCAAGTCCCGGGTTGAGTTGGAATCAGGAAAGAAGGACCTCAACACAGCCATGAACGAGCTGAACATTTTGGGAGCAGAGCTAAATGAGGTTCGAAAGGACTTGTCCGAGTCCCGGGCTAAGTTGCAATTAGGTAAGAAGGTGTGGGAGGCTGAGAAGATGGAACTGACTAAGAAGCTTAACAAGTTTGGAACAGGAAACTCTGATCTCAGAGAGGAGTTGTATGACTTGGCCAAGATCCACGAGAAGCACATTAAAGAAAATGAGAATGCGTATTGTACAGGGTACCTAACTGGGTGGTTCAGGGAGCCTCATGAATATATTTCCCTGCGAGGCTATGAGGCCAAGGAAGTTACTCTTCCGGTTCACAATCCTCTGGAGGCCACATCTTCACATTCTCATTCTCGTGCCACAATTAGACGTTTCGGAAGTGCTGGTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

427

Amino Acids

48.99

Weight (kDa)

5.74

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1125
AccB1I GGYRCC 1 cut(s) 1125
AclWI GGATC 2 cut(s) 31, 1072
AcoI YGGCCR 1 cut(s) 1071
AfaI GTAC 4 cut(s) 563, 751, 1120, 1127
AfiI CCNNNNNNNGG 2 cut(s) 849, 954
AgsI TTSAA 5 cut(s) 56, 103, 577, 607, 820
AjnI CCWGG 2 cut(s) 264, 765
Alw21I GWGCWC 2 cut(s) 9, 301
Alw26I GTCTC 2 cut(s) 579, 801
AlwI GGATC 2 cut(s) 31, 1072
Ama87I CYCGRG 2 cut(s) 849, 954
AoxI GGCC 5 cut(s) 141, 180, 1071, 1182, 1221
ApeKI GCWGC 1 cut(s) 46
Asp718I GGTACC 1 cut(s) 1125
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 4 cut(s) 181, 826, 877, 1277
AsuC2I CCSGG 4 cut(s) 850, 851, 955, 956
AsuII TTCGAA 2 cut(s) 225, 934
AvaI CYCGRG 2 cut(s) 849, 954
AvaII GGWCC 3 cut(s) 826, 877, 1277
BalI TGGCCA 1 cut(s) 1073
BanI GGYRCC 1 cut(s) 1125
BanII GRGCYC 2 cut(s) 9, 301
BauI CACGAG 2 cut(s) 1082, 1245
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 2 cut(s) 9, 301
BbvI GCAGC 1 cut(s) 58
BccI CCATC 4 cut(s) 193, 539, 730, 994
BciT130I CCWGG 2 cut(s) 266, 767
BclI TGATCA 1 cut(s) 493
BcnI CCSGG 4 cut(s) 850, 851, 955, 956
BcoDI GTCTC 2 cut(s) 579, 801
BfaI CTAG 1 cut(s) 161
BglII AGATCT 1 cut(s) 370
BisI GCNGC 1 cut(s) 47
BlsI GCNGC 1 cut(s) 48
Bme1390I CCNGG 6 cut(s) 266, 767, 850, 851, 955, 956
Bme18I GGWCC 3 cut(s) 826, 877, 1277
BmeT110I CYCGRG 2 cut(s) 849, 954
BmgT120I GGNCC 4 cut(s) 181, 826, 877, 1277
BmiI GGNNCC 5 cut(s) 86, 108, 183, 1127, 1149
BmrFI CCNGG 6 cut(s) 266, 767, 850, 851, 955, 956
BmrI ACTGGG 1 cut(s) 1143
BmsI GCATC 1 cut(s) 661
BmuI ACTGGG 1 cut(s) 1143
BpiI GAAGAC 1 cut(s) 333
BplI GAGNNNNNCTC 6 cut(s) 385, 417, 572, 604, 794, 826
BpmI CTGGAG 3 cut(s) 197, 297, 1238
Bpu10I CCTNAGC 1 cut(s) 79
Bpu14I TTCGAA 2 cut(s) 225, 934
BpuEI CTTGAG 3 cut(s) 365, 395, 407
BpuMI CCSGG 4 cut(s) 850, 851, 955, 956
BsaAI YACGTR 1 cut(s) 260
BsaJI CCNNGG 4 cut(s) 144, 849, 954, 1185
BsaWI WCCGGW 1 cut(s) 1201
BsaXI ACNNNNNCTCC 2 cut(s) 755, 785
Bsc4I CCNNNNNNNGG 2 cut(s) 849, 954
Bse1I ACTGG 1 cut(s) 1138
BseBI CCWGG 2 cut(s) 266, 767
BseDI CCNNGG 4 cut(s) 144, 849, 954, 1185
BseGI GGATG 2 cut(s) 676, 741
BseLI CCNNNNNNNGG 2 cut(s) 849, 954
BseMII CTCAG 7 cut(s) 93, 603, 684, 720, 825, 984, 1063
BseNI ACTGG 1 cut(s) 1138
BseRI GAGGAG 3 cut(s) 29, 532, 1070
BseXI GCAGC 1 cut(s) 58
BshFI GGCC 5 cut(s) 143, 182, 1073, 1184, 1223
BshNI GGYRCC 1 cut(s) 1125
BsiHKAI GWGCWC 2 cut(s) 9, 301
BsiHKCI CYCGRG 2 cut(s) 849, 954
BsiSI CCGG 3 cut(s) 850, 955, 1202
BslFI GGGAC 2 cut(s) 832, 937
BslI CCNNNNNNNGG 2 cut(s) 849, 954
BsmAI GTCTC 2 cut(s) 579, 801
BsmFI GGGAC 2 cut(s) 832, 937
BsmI GAATGC 1 cut(s) 1114
BsnI GGCC 5 cut(s) 143, 182, 1073, 1184, 1223
BsoBI CYCGRG 2 cut(s) 849, 954
Bsp119I TTCGAA 2 cut(s) 225, 934
Bsp1286I GDGCHC 2 cut(s) 9, 301
Bsp1407I TGTACA 1 cut(s) 1118
Bsp143I GATC 7 cut(s) 36, 163, 370, 463, 493, 1045, 1077
BspANI GGCC 5 cut(s) 143, 182, 1073, 1184, 1223
BspCNI CTCAG 7 cut(s) 92, 602, 685, 721, 824, 985, 1062
BspHI TCATGA 3 cut(s) 166, 508, 1153
BspLI GGNNCC 5 cut(s) 86, 108, 183, 1127, 1149
BspPI GGATC 2 cut(s) 31, 1072
BspQI GCTCTTC 1 cut(s) 162
BspT104I TTCGAA 2 cut(s) 225, 934
BspT107I GGYRCC 1 cut(s) 1125
BsrGI TGTACA 1 cut(s) 1118
BsrI ACTGG 1 cut(s) 1138
BssECI CCNNGG 4 cut(s) 144, 849, 954, 1185
BssMI GATC 7 cut(s) 36, 163, 370, 463, 493, 1045, 1077
BssSI CACGAG 2 cut(s) 1082, 1245
BssT1I CCWWGG 2 cut(s) 144, 1185
Bst2BI CACGAG 2 cut(s) 1082, 1245
Bst2UI CCWGG 2 cut(s) 266, 767
Bst6I CTCTTC 3 cut(s) 162, 386, 1203
BstAUI TGTACA 1 cut(s) 1118
BstBAI YACGTR 1 cut(s) 260
BstBI TTCGAA 2 cut(s) 225, 934
BstC8I GCNNGC 1 cut(s) 502
BstF5I GGATG 2 cut(s) 676, 741
BstHHI GCGC 1 cut(s) 637
BstKTI GATC 7 cut(s) 39, 166, 373, 466, 496, 1048, 1080
BstMAI GTCTC 2 cut(s) 579, 801
BstMBI GATC 7 cut(s) 36, 163, 370, 463, 493, 1045, 1077
BstMWI GCNNNNNNNGC 3 cut(s) 555, 716, 725
BstNI CCWGG 2 cut(s) 266, 767
BstSCI CCNGG 6 cut(s) 264, 765, 848, 849, 953, 954
BstSNI TACGTA 1 cut(s) 260
BstV1I GCAGC 1 cut(s) 58
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 3 cut(s) 36, 370, 1077
BstYI RGATCY 3 cut(s) 36, 370, 1077
BsuRI GGCC 5 cut(s) 143, 182, 1073, 1184, 1223
BtsCI GGATG 2 cut(s) 676, 741
BtsIMutI CAGTG 1 cut(s) 740
Cac8I GCNNGC 1 cut(s) 502
CciI TCATGA 3 cut(s) 166, 508, 1153
CfoI GCGC 1 cut(s) 637
Cfr13I GGNCC 4 cut(s) 181, 826, 877, 1277
Cfr9I CCCGGG 2 cut(s) 849, 954
CseI GACGC 1 cut(s) 769
Csp6I GTAC 4 cut(s) 562, 750, 1119, 1126
CviAII CATG 6 cut(s) 113, 137, 167, 509, 892, 1154
CviQI GTAC 4 cut(s) 562, 750, 1119, 1126
DpnI GATC 7 cut(s) 38, 165, 372, 465, 495, 1047, 1079
DpnII GATC 7 cut(s) 36, 163, 370, 463, 493, 1045, 1077
DraI TTTAAA 1 cut(s) 798
EaeI YGGCCR 1 cut(s) 1071
Eam1104I CTCTTC 3 cut(s) 162, 386, 1203
EarI CTCTTC 3 cut(s) 162, 386, 1203
Ecl136II GAGCTC 2 cut(s) 7, 299
Eco105I TACGTA 1 cut(s) 260
Eco130I CCWWGG 2 cut(s) 144, 1185
Eco24I GRGCYC 2 cut(s) 9, 301
Eco47I GGWCC 3 cut(s) 826, 877, 1277
Eco53kI GAGCTC 2 cut(s) 7, 299
Eco88I CYCGRG 2 cut(s) 849, 954
EcoICRI GAGCTC 2 cut(s) 7, 299
EcoO109I RGGNCCY 2 cut(s) 181, 877
EcoRII CCWGG 2 cut(s) 264, 765
EcoT14I CCWWGG 2 cut(s) 144, 1185
EcoT38I GRGCYC 2 cut(s) 9, 301
ErhI CCWWGG 2 cut(s) 144, 1185
FaeI CATG 6 cut(s) 116, 140, 170, 512, 895, 1157
FalI AAGNNNNNCTT 4 cut(s) 670, 702, 1182, 1214
FaqI GGGAC 2 cut(s) 832, 937
FatI CATG 6 cut(s) 112, 136, 166, 508, 891, 1153
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 1 cut(s) 47
FokI GGATG 2 cut(s) 683, 748
FriOI GRGCYC 2 cut(s) 9, 301
Fsp4HI GCNGC 1 cut(s) 47
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 636
GluI GCNGC 1 cut(s) 47
GsuI CTGGAG 3 cut(s) 197, 297, 1238
HaeIII GGCC 5 cut(s) 143, 182, 1073, 1184, 1223
HapII CCGG 3 cut(s) 850, 955, 1202
HgaI GACGC 1 cut(s) 769
HhaI GCGC 1 cut(s) 637
Hin1II CATG 6 cut(s) 116, 140, 170, 512, 895, 1157
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HindIII AAGCTT 2 cut(s) 792, 1016
HinfI GANTC 8 cut(s) 308, 475, 512, 587, 770, 809, 863, 950
HpaII CCGG 3 cut(s) 850, 955, 1202
Hpy166II GTNNAC 1 cut(s) 1207
Hpy188I TCNGA 5 cut(s) 25, 949, 1045, 1052, 1268
Hpy8I GTNNAC 1 cut(s) 1207
HpyAV CCTTC 4 cut(s) 571, 814, 868, 973
HpyCH4IV ACGT 3 cut(s) 150, 259, 1261
HpyCH4V TGCA 1 cut(s) 967
HpyF10VI GCNNNNNNNGC 3 cut(s) 555, 716, 725
HpySE526I ACGT 3 cut(s) 150, 259, 1261
Hsp92II CATG 6 cut(s) 116, 140, 170, 512, 895, 1157
HspAI GCGC 1 cut(s) 635
KpnI GGTACC 1 cut(s) 1129
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 7 cut(s) 36, 163, 370, 463, 493, 1045, 1077
LguI GCTCTTC 1 cut(s) 162
LmnI GCTCC 6 cut(s) 4, 580, 617, 802, 914, 1147
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 1 cut(s) 661
MaeI CTAG 1 cut(s) 161
MaeII ACGT 3 cut(s) 150, 259, 1261
MaeIII GTNAC 1 cut(s) 1192
MalI GATC 7 cut(s) 38, 165, 372, 465, 495, 1047, 1079
MboI GATC 7 cut(s) 36, 163, 370, 463, 493, 1045, 1077
MboII GAAGA 9 cut(s) 46, 149, 286, 338, 403, 473, 1009, 1190, 1221
MflI RGATCY 3 cut(s) 36, 370, 1077
MhlI GDGCHC 2 cut(s) 9, 301
MlsI TGGCCA 1 cut(s) 1073
MluCI AATT 7 cut(s) 213, 232, 525, 650, 753, 968, 1254
MluNI TGGCCA 1 cut(s) 1073
MlyI GAGTC 5 cut(s) 521, 581, 764, 803, 959
MmeI TCCRAC 1 cut(s) 840
Mox20I TGGCCA 1 cut(s) 1073
MscI TGGCCA 1 cut(s) 1073
MseI TTAA 5 cut(s) 212, 216, 797, 1020, 1095
MslI CAYNNNNRTG 2 cut(s) 890, 1245
Msp20I TGGCCA 1 cut(s) 1073
MspI CCGG 3 cut(s) 850, 955, 1202
MspR9I CCNGG 6 cut(s) 266, 767, 850, 851, 955, 956
Mva1269I GAATGC 1 cut(s) 1114
MvaI CCWGG 2 cut(s) 266, 767
MwoI GCNNNNNNNGC 3 cut(s) 555, 716, 725
NciI CCSGG 4 cut(s) 850, 851, 955, 956
NdeII GATC 7 cut(s) 36, 163, 370, 463, 493, 1045, 1077
NlaIII CATG 6 cut(s) 116, 140, 170, 512, 895, 1157
NlaIV GGNNCC 5 cut(s) 86, 108, 183, 1127, 1149
NspV TTCGAA 2 cut(s) 225, 934
PacI TTAATTAA 1 cut(s) 216
PagI TCATGA 3 cut(s) 166, 508, 1153
PciSI GCTCTTC 1 cut(s) 162
PctI GAATGC 1 cut(s) 1114
PfeI GAWTC 3 cut(s) 308, 475, 863
PkrI GCNGC 1 cut(s) 48
PleI GAGTC 5 cut(s) 520, 581, 764, 803, 958
PpsI GAGTC 5 cut(s) 520, 581, 764, 803, 958
Ppu21I YACGTR 1 cut(s) 260
PpuMI RGGWCCY 1 cut(s) 877
Psp124BI GAGCTC 2 cut(s) 9, 301
Psp5II RGGWCCY 1 cut(s) 877
Psp6I CCWGG 2 cut(s) 264, 765
PspGI CCWGG 2 cut(s) 264, 765
PspN4I GGNNCC 5 cut(s) 86, 108, 183, 1127, 1149
PspPI GGNCC 4 cut(s) 181, 826, 877, 1277
PspPPI RGGWCCY 1 cut(s) 877
PsuI RGATCY 3 cut(s) 36, 370, 1077
RsaI GTAC 4 cut(s) 563, 751, 1120, 1127
RsaNI GTAC 4 cut(s) 562, 750, 1119, 1126
RseI CAYNNNNRTG 2 cut(s) 890, 1245
SacI GAGCTC 2 cut(s) 9, 301
SapI GCTCTTC 1 cut(s) 162
SaqAI TTAA 5 cut(s) 212, 216, 797, 1020, 1095
SatI GCNGC 1 cut(s) 47
Sau3AI GATC 7 cut(s) 36, 163, 370, 463, 493, 1045, 1077
Sau96I GGNCC 4 cut(s) 181, 826, 877, 1277
SchI GAGTC 5 cut(s) 521, 581, 764, 803, 959
ScrFI CCNGG 6 cut(s) 266, 767, 850, 851, 955, 956
SduI GDGCHC 2 cut(s) 9, 301
SfaNI GCATC 1 cut(s) 661
SfuI TTCGAA 2 cut(s) 225, 934
SinI GGWCC 3 cut(s) 826, 877, 1277
SmaI CCCGGG 2 cut(s) 851, 956
SmiMI CAYNNNNRTG 2 cut(s) 890, 1245
SmlI CTYRAG 3 cut(s) 344, 374, 386
SmoI CTYRAG 3 cut(s) 344, 374, 386
SnaBI TACGTA 1 cut(s) 260
Sse9I AATT 7 cut(s) 213, 232, 525, 650, 753, 968, 1254
SspMI CTAG 1 cut(s) 161
SstI GAGCTC 2 cut(s) 9, 301
StyD4I CCNGG 6 cut(s) 264, 765, 848, 849, 953, 954
StyI CCWWGG 2 cut(s) 144, 1185
TaiI ACGT 3 cut(s) 153, 262, 1264
TaqI TCGA 3 cut(s) 225, 515, 934
TasI AATT 7 cut(s) 213, 232, 525, 650, 753, 968, 1254
TatI WGTACW 3 cut(s) 561, 749, 1118
TfiI GAWTC 3 cut(s) 308, 475, 863
Tru1I TTAA 5 cut(s) 212, 216, 797, 1020, 1095
Tru9I TTAA 5 cut(s) 212, 216, 797, 1020, 1095
TscAI CASTG 1 cut(s) 747
TseI GCWGC 1 cut(s) 46
TspDTI ATGAA 5 cut(s) 183, 227, 474, 908, 1170
TspMI CCCGGG 2 cut(s) 849, 954
TspRI CASTG 1 cut(s) 747
VpaK11BI GGWCC 3 cut(s) 826, 877, 1277
XbaI TCTAGA 1 cut(s) 160
XmaI CCCGGG 2 cut(s) 849, 954
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.