RchiOBHm_Chr4g0411131

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003527
Physical Location & Seq
Forward (+)
34936118 .. 34936540
423 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 342 bp
ATGGCCAACATCATCAAGAGGATAAAGCTCCGGTCACAAGGCCTGGAGGTCTCGGCTCAAGGACTGGGTTGCATGGGCATGTCCGTCCCCCTAAAGCCTGAACCCAACATGATCAATCTCATCCACCACGCCGTCGACGTTGGCATCATCTTCCTTGATACCGTTGACGTTTATGGTCCCTTCACCAACAAACTTCTTCTTGACAAGGCTTTGATTGGAGGAGAAAGAGACAAGGTTGAACTGGCCACCAAATTTGCTATCAACTTTGAGGACGACAAGAGAGAGATTATTCGTGGCGAGCCAGCATATGTGAGAGTTTCTTGTGAAGGAAGCTTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

113

Amino Acids

12.48

Weight (kDa)

6.72

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 33 - 113 1.7e-07 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AcoI YGGCCR 2 cut(s) 3, 243
AcsI RAATTY 1 cut(s) 251
AgsI TTSAA 2 cut(s) 239, 337
AjnI CCWGG 1 cut(s) 42
AluBI AGCT 2 cut(s) 28, 333
AluI AGCT 2 cut(s) 28, 333
Alw26I GTCTC 2 cut(s) 55, 222
AoxI GGCC 3 cut(s) 3, 40, 243
ApoI RAATTY 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 175
AvaII GGWCC 1 cut(s) 176
BalI TGGCCA 2 cut(s) 5, 245
BceAI ACGGC 1 cut(s) 116
BciT130I CCWGG 1 cut(s) 44
BclI TGATCA 1 cut(s) 111
BcoDI GTCTC 2 cut(s) 55, 222
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 1 cut(s) 44
BmrI ACTGGG 1 cut(s) 74
BmsI GCATC 1 cut(s) 153
BmuI ACTGGG 1 cut(s) 74
BpmI CTGGAG 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 55
BsaWI WCCGGW 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 69, 246
BseBI CCWGG 1 cut(s) 44
BseGI GGATG 1 cut(s) 120
BseNI ACTGG 2 cut(s) 69, 246
BseRI GAGGAG 1 cut(s) 234
BshFI GGCC 3 cut(s) 5, 42, 245
BsiSI CCGG 1 cut(s) 31
BslFI GGGAC 2 cut(s) 71, 162
BsmAI GTCTC 2 cut(s) 55, 222
BsmFI GGGAC 2 cut(s) 71, 162
BsnI GGCC 3 cut(s) 5, 42, 245
Bso31I GGTCTC 1 cut(s) 55
Bsp143I GATC 1 cut(s) 111
BspANI GGCC 3 cut(s) 5, 42, 245
BspLI GGNNCC 1 cut(s) 178
BspTNI GGTCTC 1 cut(s) 55
BsrI ACTGG 2 cut(s) 69, 246
BssMI GATC 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 1 cut(s) 163
BstC8I GCNNGC 2 cut(s) 299, 303
BstF5I GGATG 1 cut(s) 120
BstKTI GATC 1 cut(s) 114
BstMAI GTCTC 2 cut(s) 55, 222
BstMBI GATC 1 cut(s) 111
BstNI CCWGG 1 cut(s) 44
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 42
BsuRI GGCC 3 cut(s) 5, 42, 245
BtsCI GGATG 1 cut(s) 120
Cac8I GCNNGC 2 cut(s) 299, 303
Cfr13I GGNCC 1 cut(s) 176
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 3 cut(s) 73, 79, 109
CviJI RGCY 9 cut(s) 5, 28, 42, 56, 97, 209, 245, 301, 333
CviKI_1 RGCY 9 cut(s) 5, 28, 42, 56, 97, 209, 245, 301, 333
DpnI GATC 1 cut(s) 113
DpnII GATC 1 cut(s) 111
EaeI YGGCCR 2 cut(s) 3, 243
Eco147I AGGCCT 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 42
FaeI CATG 3 cut(s) 76, 82, 112
FaiI YATR 6 cut(s) 74, 80, 110, 174, 307, 309
FaqI GGGAC 2 cut(s) 71, 162
FatI CATG 3 cut(s) 72, 78, 108
FauNDI CATATG 1 cut(s) 307
FbaI TGATCA 1 cut(s) 111
FblI GTMKAC 1 cut(s) 135
FokI GGATG 1 cut(s) 107
GsuI CTGGAG 1 cut(s) 65
HaeIII GGCC 3 cut(s) 5, 42, 245
HapII CCGG 1 cut(s) 31
Hin1II CATG 3 cut(s) 76, 82, 112
HincII GTYRAC 2 cut(s) 136, 166
HindII GTYRAC 2 cut(s) 136, 166
HindIII AAGCTT 1 cut(s) 331
HpaII CCGG 1 cut(s) 31
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 2 cut(s) 136, 166
Hpy188III TCNNGA 2 cut(s) 16, 200
Hpy8I GTNNAC 2 cut(s) 136, 166
Hpy99I CGWCG 2 cut(s) 137, 140
HpyAV CCTTC 2 cut(s) 190, 320
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4IV ACGT 2 cut(s) 138, 168
HpyCH4V TGCA 1 cut(s) 72
HpySE526I ACGT 2 cut(s) 138, 168
Hsp92II CATG 3 cut(s) 76, 82, 112
Ksp22I TGATCA 1 cut(s) 111
Kzo9I GATC 1 cut(s) 111
LmnI GCTCC 1 cut(s) 33
LpnPI CCDG 7 cut(s) 29, 44, 50, 56, 111, 227, 315
LweI GCATC 1 cut(s) 153
MaeII ACGT 2 cut(s) 138, 168
MaeIII GTNAC 1 cut(s) 33
MalI GATC 1 cut(s) 113
MboI GATC 1 cut(s) 111
MboII GAAGA 2 cut(s) 142, 188
MlsI TGGCCA 2 cut(s) 5, 245
MluCI AATT 1 cut(s) 251
MluNI TGGCCA 2 cut(s) 5, 245
MnlI CCTC 4 cut(s) 12, 40, 212, 262
Mox20I TGGCCA 2 cut(s) 5, 245
MscI TGGCCA 2 cut(s) 5, 245
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 2 cut(s) 5, 245
MspI CCGG 1 cut(s) 31
MspR9I CCNGG 1 cut(s) 44
MvaI CCWGG 1 cut(s) 44
NdeI CATATG 1 cut(s) 307
NdeII GATC 1 cut(s) 111
NlaIII CATG 3 cut(s) 76, 82, 112
NlaIV GGNNCC 1 cut(s) 178
NmeAIII GCCGAG 1 cut(s) 32
NmuCI GTSAC 1 cut(s) 33
NspI RCATGY 1 cut(s) 82
PceI AGGCCT 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 42
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 1 cut(s) 176
RseI CAYNNNNRTG 1 cut(s) 77
SalI GTCGAC 1 cut(s) 134
Sau3AI GATC 1 cut(s) 111
Sau96I GGNCC 1 cut(s) 176
ScrFI CCNGG 1 cut(s) 44
SetI ASST 6 cut(s) 30, 51, 141, 171, 237, 335
SfaNI GCATC 1 cut(s) 153
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
Sse9I AATT 1 cut(s) 251
SseBI AGGCCT 1 cut(s) 42
StuI AGGCCT 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 42
TaaI ACNGT 1 cut(s) 163
TaiI ACGT 2 cut(s) 141, 171
TaqI TCGA 1 cut(s) 135
TasI AATT 1 cut(s) 251
TseFI GTSAC 1 cut(s) 33
Tsp45I GTSAC 1 cut(s) 33
TspGWI ACGGA 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 176
XapI RAATTY 1 cut(s) 251
XceI RCATGY 1 cut(s) 82
XmiI GTMKAC 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.