Rh4AG164300

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003527
Physical Location & Seq
Forward (+)
41553585 .. 41572073
18489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG164300.1

Sequence Viewer

Length: 465 bp
ATGAGGAGGATGAAGCTCGGGTCACAAGGCCTGGAGGTCTCAGCACAAGGACTGGGCTGCATGGGCATGTCTGCCTTCTACGGCGCCCCGAAGCCCGAACCCGACATGATCAATCTCATCCACCACGCCATCGACTCCGGTGTCACCTTCCTCGACACCGCCGACCTGTACGGTCCCCACACCAACGAAATTCTCCTCGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAACTGGCCACCAAATTCGGTATCAGGTTTGCGGACAACAAAATGGAGGTTCAAGGCGACCCGGCATATGTGAGAGCTGCTTGTGAAGAAAGCTTGAAACGCCTTGGTGTTGCTTCTGTTGATCTCTGTTATCAGATTAGGATTGACACCCGTGTCCCCATTGAAGTCACGAGATGTATAGTACATCCAAATCCTCAGAAGATGATTACATGTTCTACCATGGAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

154

Amino Acids

16.83

Weight (kDa)

6.41

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 20 - 133 4.6e-22 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 140, 386
AccB1I GGYRCC 1 cut(s) 83
AciI CCGC 2 cut(s) 159, 266
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 2 cut(s) 189, 248
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 2 cut(s) 170, 417
AfiI CCNNNNNNNGG 1 cut(s) 202
AflIII ACRYGT 1 cut(s) 443
AgsI TTSAA 4 cut(s) 236, 287, 331, 398
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 3 cut(s) 16, 311, 327
AluI AGCT 3 cut(s) 16, 311, 327
Alw26I GTCTC 2 cut(s) 43, 219
Ama87I CYCGRG 1 cut(s) 17
AoxI GGCC 2 cut(s) 28, 240
ApeKI GCWGC 2 cut(s) 57, 311
ApoI RAATTY 2 cut(s) 189, 248
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 173
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 136, 232
AvaI CYCGRG 1 cut(s) 17
AvaII GGWCC 1 cut(s) 173
BalI TGGCCA 1 cut(s) 242
BanI GGYRCC 1 cut(s) 83
BauI CACGAG 1 cut(s) 403
BbvI GCAGC 2 cut(s) 44, 298
BccI CCATC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 1 cut(s) 32
BclI TGATCA 1 cut(s) 108
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 2 cut(s) 43, 219
BfoI RGCGCY 1 cut(s) 87
BisI GCNGC 2 cut(s) 58, 312
BlsI GCNGC 2 cut(s) 59, 313
Bme1390I CCNGG 2 cut(s) 32, 296
Bme18I GGWCC 1 cut(s) 173
BmeT110I CYCGRG 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 2 cut(s) 85, 175
BmrFI CCNGG 2 cut(s) 32, 296
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
BpmI CTGGAG 1 cut(s) 53
BpuMI CCSGG 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 3 cut(s) 196, 337, 453
BsaWI WCCGGW 1 cut(s) 137
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 57, 243
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 3 cut(s) 196, 337, 453
BseGI GGATG 3 cut(s) 15, 117, 418
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMII CTCAG 2 cut(s) 54, 443
BseNI ACTGG 2 cut(s) 57, 243
BseRI GAGGAG 2 cut(s) 19, 185
BseXI GCAGC 2 cut(s) 44, 298
BshFI GGCC 2 cut(s) 30, 242
BshNI GGYRCC 1 cut(s) 83
BsiHKCI CYCGRG 1 cut(s) 17
BsiSI CCGG 2 cut(s) 138, 296
BslFI GGGAC 2 cut(s) 159, 374
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 2 cut(s) 43, 219
BsmFI GGGAC 2 cut(s) 159, 374
BsnI GGCC 2 cut(s) 30, 242
Bso31I GGTCTC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 17
Bsp143I GATC 2 cut(s) 108, 355
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 2 cut(s) 159, 266
BspANI GGCC 2 cut(s) 30, 242
BspCNI CTCAG 2 cut(s) 53, 442
BspLI GGNNCC 2 cut(s) 85, 175
BspT107I GGYRCC 1 cut(s) 83
BspTNI GGTCTC 1 cut(s) 43
BsrI ACTGG 2 cut(s) 57, 243
BssECI CCNNGG 3 cut(s) 196, 337, 453
BssMI GATC 2 cut(s) 108, 355
BssNI GRCGYC 1 cut(s) 84
BssSI CACGAG 1 cut(s) 403
BssT1I CCWWGG 2 cut(s) 337, 453
Bst2BI CACGAG 1 cut(s) 403
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 1 cut(s) 173
BstACI GRCGYC 1 cut(s) 84
BstDEI CTNAG 2 cut(s) 40, 429
BstDSI CCRYGG 1 cut(s) 453
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 3 cut(s) 15, 117, 418
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 2 cut(s) 111, 358
BstMAI GTCTC 2 cut(s) 43, 219
BstMBI GATC 2 cut(s) 108, 355
BstMWI GCNNNNNNNGC 2 cut(s) 63, 333
BstNI CCWGG 1 cut(s) 32
BstNSI RCATGY 2 cut(s) 70, 447
BstSCI CCNGG 2 cut(s) 30, 294
BstV1I GCAGC 2 cut(s) 44, 298
BsuRI GGCC 2 cut(s) 30, 242
BtgI CCRYGG 1 cut(s) 453
BtsCI GGATG 3 cut(s) 15, 117, 418
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 173
Csp6I GTAC 2 cut(s) 169, 416
CviAII CATG 5 cut(s) 61, 67, 106, 444, 454
CviJI RGCY 8 cut(s) 16, 30, 57, 94, 206, 242, 311, 327
CviKI_1 RGCY 8 cut(s) 16, 30, 57, 94, 206, 242, 311, 327
CviQI GTAC 2 cut(s) 169, 416
DdeI CTNAG 2 cut(s) 40, 429
DinI GGCGCC 1 cut(s) 85
DpnI GATC 2 cut(s) 110, 357
DpnII GATC 2 cut(s) 108, 355
DrdI GACNNNNNNGTC 2 cut(s) 140, 386
DseDI GACNNNNNNGTC 2 cut(s) 140, 386
EaeI YGGCCR 1 cut(s) 240
Eco130I CCWWGG 2 cut(s) 337, 453
Eco147I AGGCCT 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 17
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 2 cut(s) 337, 453
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
ErhI CCWWGG 2 cut(s) 337, 453
FaeI CATG 5 cut(s) 64, 70, 109, 447, 457
FaiI YATR 8 cut(s) 62, 68, 107, 301, 303, 413, 445, 455
FaqI GGGAC 2 cut(s) 159, 374
FatI CATG 5 cut(s) 60, 66, 105, 443, 453
FauNDI CATATG 1 cut(s) 301
FbaI TGATCA 1 cut(s) 108
Fnu4HI GCNGC 2 cut(s) 58, 312
FokI GGATG 3 cut(s) 22, 104, 405
Fsp4HI GCNGC 2 cut(s) 58, 312
GlaI GCGC 1 cut(s) 85
GluI GCNGC 2 cut(s) 58, 312
GsuI CTGGAG 1 cut(s) 53
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 2 cut(s) 30, 242
HapII CCGG 2 cut(s) 138, 296
HhaI GCGC 1 cut(s) 86
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 5 cut(s) 64, 70, 109, 447, 457
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 1 cut(s) 134
HpaII CCGG 2 cut(s) 138, 296
HphI GGTGA 2 cut(s) 136, 232
Hpy188I TCNGA 2 cut(s) 369, 432
Hpy188III TCNNGA 1 cut(s) 403
HpyAV CCTTC 2 cut(s) 85, 157
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 1 cut(s) 60
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 333
HpyF3I CTNAG 2 cut(s) 40, 429
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 5 cut(s) 64, 70, 109, 447, 457
HspAI GCGC 1 cut(s) 84
KasI GGCGCC 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 108
Kzo9I GATC 2 cut(s) 108, 355
LpnPI CCDG 8 cut(s) 17, 38, 44, 151, 179, 224, 244, 309
Lsp1109I GCAGC 2 cut(s) 44, 298
MaeIII GTNAC 3 cut(s) 21, 142, 400
MalI GATC 2 cut(s) 110, 357
MboI GATC 2 cut(s) 108, 355
MboII GAAGA 2 cut(s) 332, 445
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 2 cut(s) 189, 248
MluNI TGGCCA 1 cut(s) 242
Mly113I GGCGCC 1 cut(s) 84
MlyI GAGTC 1 cut(s) 128
MnlI CCTC 7 cut(s) 28, 161, 206, 209, 274, 438, 451
Mox20I TGGCCA 1 cut(s) 242
MscI TGGCCA 1 cut(s) 242
MslI CAYNNNNRTG 1 cut(s) 65
Msp20I TGGCCA 1 cut(s) 242
MspI CCGG 2 cut(s) 138, 296
MspR9I CCNGG 2 cut(s) 32, 296
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 2 cut(s) 63, 333
NarI GGCGCC 1 cut(s) 84
NciI CCSGG 1 cut(s) 296
NcoI CCATGG 1 cut(s) 453
NdeI CATATG 1 cut(s) 301
NdeII GATC 2 cut(s) 108, 355
NlaIII CATG 5 cut(s) 64, 70, 109, 447, 457
NlaIV GGNNCC 2 cut(s) 85, 175
NmeAIII GCCGAG 1 cut(s) 177
NmuCI GTSAC 3 cut(s) 21, 142, 400
NspI RCATGY 2 cut(s) 70, 447
PceI AGGCCT 1 cut(s) 30
PciI ACATGT 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PkrI GCNGC 2 cut(s) 59, 313
PleI GAGTC 1 cut(s) 128
PluTI GGCGCC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 128
PscI ACATGT 1 cut(s) 443
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 85, 175
PspPI GGNCC 1 cut(s) 173
RsaI GTAC 2 cut(s) 170, 417
RsaNI GTAC 2 cut(s) 169, 416
RseI CAYNNNNRTG 1 cut(s) 65
SatI GCNGC 2 cut(s) 58, 312
Sau3AI GATC 2 cut(s) 108, 355
Sau96I GGNCC 1 cut(s) 173
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 2 cut(s) 32, 296
SfoI GGCGCC 1 cut(s) 85
SinI GGWCC 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 65
Sse9I AATT 2 cut(s) 189, 248
SseBI AGGCCT 1 cut(s) 30
SsiI CCGC 2 cut(s) 159, 266
SspDI GGCGCC 1 cut(s) 83
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 2 cut(s) 30, 294
StyI CCWWGG 2 cut(s) 337, 453
TaaI ACNGT 1 cut(s) 173
TaqI TCGA 2 cut(s) 132, 153
TasI AATT 2 cut(s) 189, 248
TatI WGTACW 1 cut(s) 415
TseFI GTSAC 3 cut(s) 21, 142, 400
TseI GCWGC 2 cut(s) 57, 311
Tsp45I GTSAC 3 cut(s) 21, 142, 400
TspDTI ATGAA 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 173
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 2 cut(s) 189, 248
XceI RCATGY 2 cut(s) 70, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.