Rh4DG159000

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003527
Physical Location & Seq
Reverse (-)
31671699 .. 31672357
659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG159000.1

Sequence Viewer

Length: 468 bp
ATGGCGAGCATCAGGAGGATGAAGCTGGGGTCACAAGGCCTGGAGGTCTCGGCTCAAGGGCTGGGTTGCGTGGGCATGTCCGTCTTCTCTGGCCCCCCGAAGCCTGAAGTCGACATGATCAAGCTCATCCACCACGCCATCGACGCCGGCGTCGCCTTCCTTGATACCGCTGACGTTTACGGTCCCTTCACCAACGAACTTCTTCTTGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAATTGGCCACCAAATTTGGTATCAGCTTTGAGGACAGCAAGAGAGAGATTATTCGAGGCGACCCGGCATATGTCAGGTCTGCTTGTGAAGGAAGCTTGAAACGACTTGGTGTAGATTCTATTGATCTCTATTATCAGCATCGGATTGACACTCGTGTCCCCATCGAAGTCACGGCTGTCAGTGCCTTTACAAATCTTGTTAGAGTTGTAAATGTGAGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

155

Amino Acids

16.81

Weight (kDa)

7.79

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 24 - 140 4.8e-22 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 149, 398
AccI GTMKAC 1 cut(s) 111
AciI CCGC 1 cut(s) 168
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 257
AcuI CTGAAG 1 cut(s) 126
AcyI GRCGYC 2 cut(s) 144, 150
AgsI TTSAA 2 cut(s) 245, 343
AjnI CCWGG 1 cut(s) 39
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AluBI AGCT 4 cut(s) 25, 124, 270, 339
AluI AGCT 4 cut(s) 25, 124, 270, 339
Alw26I GTCTC 2 cut(s) 52, 228
AoxI GGCC 3 cut(s) 37, 91, 249
ApoI RAATTY 1 cut(s) 257
Asp700I GAANNNNTTC 1 cut(s) 201
AspS9I GGNCC 2 cut(s) 92, 182
AsuC2I CCSGG 1 cut(s) 308
AsuHPI GGTGA 2 cut(s) 181, 241
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 251
BauI CACGAG 1 cut(s) 396
BbsI GAAGAC 1 cut(s) 76
BccI CCATC 2 cut(s) 146, 413
BceAI ACGGC 1 cut(s) 432
BciT130I CCWGG 1 cut(s) 41
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 1 cut(s) 308
BcoDI GTCTC 2 cut(s) 52, 228
BfaI CTAG 1 cut(s) 466
Bme1390I CCNGG 2 cut(s) 41, 308
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 2 cut(s) 92, 182
BmiI GGNNCC 2 cut(s) 94, 184
BmrFI CCNGG 2 cut(s) 41, 308
BmsI GCATC 2 cut(s) 18, 391
BpiI GAAGAC 1 cut(s) 76
BpmI CTGGAG 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 39
BpuMI CCSGG 1 cut(s) 308
BsaHI GRCGYC 2 cut(s) 144, 150
BsaI GGTCTC 1 cut(s) 52
Bse118I RCCGGY 1 cut(s) 146
BseBI CCWGG 1 cut(s) 41
BseGI GGATG 2 cut(s) 24, 126
BseYI CCCAGC 2 cut(s) 25, 61
BshFI GGCC 3 cut(s) 39, 93, 251
BsiSI CCGG 2 cut(s) 147, 308
BslFI GGGAC 2 cut(s) 168, 386
BsmAI GTCTC 2 cut(s) 52, 228
BsmFI GGGAC 2 cut(s) 168, 386
BsnI GGCC 3 cut(s) 39, 93, 251
Bso31I GGTCTC 1 cut(s) 52
Bsp143I GATC 2 cut(s) 117, 367
BspACI CCGC 1 cut(s) 168
BspANI GGCC 3 cut(s) 39, 93, 251
BspLI GGNNCC 2 cut(s) 94, 184
BspTNI GGTCTC 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 146
BssAI RCCGGY 1 cut(s) 146
BssMI GATC 2 cut(s) 117, 367
BssNI GRCGYC 2 cut(s) 144, 150
BssSI CACGAG 1 cut(s) 396
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 182
BstACI GRCGYC 2 cut(s) 144, 150
BstC8I GCNNGC 2 cut(s) 7, 148
BstF5I GGATG 2 cut(s) 24, 126
BstKTI GATC 2 cut(s) 120, 370
BstMAI GTCTC 2 cut(s) 52, 228
BstMBI GATC 2 cut(s) 117, 367
BstMWI GCNNNNNNNGC 3 cut(s) 143, 152, 425
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 2 cut(s) 39, 306
BstV2I GAAGAC 1 cut(s) 76
BsuRI GGCC 3 cut(s) 39, 93, 251
BtsCI GGATG 2 cut(s) 24, 126
BtsIMutI CAGTG 1 cut(s) 430
Cac8I GCNNGC 2 cut(s) 7, 148
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 92, 182
CseI GACGC 2 cut(s) 139, 152
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviAII CATG 2 cut(s) 76, 115
DpnI GATC 2 cut(s) 119, 369
DpnII GATC 2 cut(s) 117, 367
DrdI GACNNNNNNGTC 2 cut(s) 149, 398
DseDI GACNNNNNNGTC 2 cut(s) 149, 398
EaeI YGGCCR 1 cut(s) 249
Eco147I AGGCCT 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 126
EcoRII CCWGG 1 cut(s) 39
FaeI CATG 2 cut(s) 79, 118
FaiI YATR 4 cut(s) 77, 116, 313, 315
FaqI GGGAC 2 cut(s) 168, 386
FatI CATG 2 cut(s) 75, 114
FauNDI CATATG 1 cut(s) 313
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 111
FokI GGATG 2 cut(s) 31, 113
FspBI CTAG 1 cut(s) 466
GsaI CCCAGC 2 cut(s) 29, 65
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 39, 93, 251
HapII CCGG 2 cut(s) 147, 308
HgaI GACGC 2 cut(s) 139, 152
Hin1I GRCGYC 2 cut(s) 144, 150
Hin1II CATG 2 cut(s) 79, 118
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 1 cut(s) 359
HpaII CCGG 2 cut(s) 147, 308
HphI GGTGA 2 cut(s) 181, 241
Hpy166II GTNNAC 2 cut(s) 112, 178
Hpy188I TCNGA 1 cut(s) 387
Hpy188III TCNNGA 1 cut(s) 13
Hpy8I GTNNAC 2 cut(s) 112, 178
Hpy99I CGWCG 2 cut(s) 146, 155
HpyAV CCTTC 3 cut(s) 166, 196, 326
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 174
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 152, 425
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 2 cut(s) 144, 150
Hsp92II CATG 2 cut(s) 79, 118
KroI GCCGGC 1 cut(s) 146
KroNI GCCGGC 1 cut(s) 148
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 2 cut(s) 117, 367
LpnPI CCDG 9 cut(s) 11, 26, 47, 53, 75, 117, 160, 304, 321
LweI GCATC 2 cut(s) 18, 391
MaeI CTAG 1 cut(s) 466
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 2 cut(s) 30, 412
MalI GATC 2 cut(s) 119, 369
MboI GATC 2 cut(s) 117, 367
MboII GAAGA 2 cut(s) 76, 194
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 2 cut(s) 245, 257
MluNI TGGCCA 1 cut(s) 251
MnlI CCTC 5 cut(s) 9, 37, 218, 268, 293
Mox20I TGGCCA 1 cut(s) 251
MreI CGCCGGCG 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 201
MscI TGGCCA 1 cut(s) 251
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 2 cut(s) 147, 308
MspR9I CCNGG 2 cut(s) 41, 308
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 3 cut(s) 143, 152, 425
NaeI GCCGGC 1 cut(s) 148
NciI CCSGG 1 cut(s) 308
NdeI CATATG 1 cut(s) 313
NdeII GATC 2 cut(s) 117, 367
NgoMIV GCCGGC 1 cut(s) 146
NlaIII CATG 2 cut(s) 79, 118
NlaIV GGNNCC 2 cut(s) 94, 184
NmeAIII GCCGAG 1 cut(s) 29
NmuCI GTSAC 2 cut(s) 30, 412
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PdiI GCCGGC 1 cut(s) 148
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 359
Psp6I CCWGG 1 cut(s) 39
PspFI CCCAGC 2 cut(s) 25, 61
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 94, 184
PspPI GGNCC 2 cut(s) 92, 182
SalI GTCGAC 1 cut(s) 110
Sau3AI GATC 2 cut(s) 117, 367
Sau96I GGNCC 2 cut(s) 92, 182
ScrFI CCNGG 2 cut(s) 41, 308
SetI ASST 8 cut(s) 27, 48, 126, 177, 243, 272, 323, 341
SfaNI GCATC 2 cut(s) 18, 391
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 2 cut(s) 245, 257
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 1 cut(s) 168
SspMI CTAG 1 cut(s) 466
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 39, 306
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 1 cut(s) 177
TaqI TCGA 4 cut(s) 111, 141, 298, 408
TasI AATT 2 cut(s) 245, 257
TfiI GAWTC 1 cut(s) 359
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 2 cut(s) 30, 412
Tsp45I GTSAC 2 cut(s) 30, 412
TspDTI ATGAA 1 cut(s) 35
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 1 cut(s) 430
VpaK11BI GGWCC 1 cut(s) 182
XapI RAATTY 1 cut(s) 257
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.