AT1G60730

aldo-keto reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000435
Physical Location & Seq
Reverse (-)
22357976 .. 22360277
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G60730.1

Sequence Viewer

Length: 1038 bp
ATGGCGGAAGCTTGCGGAGTGAGAAGGATTAAGCTAGGAAGCCAAGGCCTTGAGGTATCGGCGCAAGGTCTTGGTTGCATGGGTCTCTCTGCTTTCTACGGTACTCCCAAGCCGGAAACCGAAGCTATCGCTCTCATCCACCACGCCATTCACTCCGGCGTCACTTTCCTTGACACCTCCGATATCTACGGTCCTGAGACCAACGAGCTTCTCCTCAGCAAGGCTCTCAAGGATGGGGTGAGAGAAAAAGTGGAACTTGCGACCAAATATGGAATCAGGTATGCAGAGGGGAAAGTAGAGTTTAAGGGAGATCCTGCTTATGTAAGAGCTGCGTGTGAGGCTAGTTTAATGCGAGTAGATGTTGCCTGCATTGATCTCTATTATCAGCATCGAATTGATACTCGTGTCCCTATCGAAATCACTATTGGAGAGCTGAAGAAGCTAGTTGAAGAAGGTAAAATAAAGTATATCGGTTTGTCTGAAGCTTCAGCTTCAACTATCAGAAGAGCACATGCTGTTCACCCGATAACCGCTCTGCAAATAGAATGGTCCTTATGGTCGAGAGACGTGGAAGAAGATATAATTCCAACCTGCAGGGAACTTGGGATTGGAATTGTTGCTTACAGTCCTCTGGGACGAGGTTTCTTTGCTTCAGGACCCAAGCTTGTTGAGAACCTAGACAATAATGACGTCAGAAAGACTCTACCAAGATTCCAACAGGAAAACTTAGACCACAACAAGATTCTCTTTGAGAAGGTTTCTGCAATGTCTGAGAAGAAAGGATGCACTCCTGCACAACTAGCCCTTGCATGGGTTCATCACCAGGGAGATGATGTTTGCCCCATCCCAGGAACCACTAAGATTGAAAACCTCAACCAGAACATTGGAGCTTTATCAGTGAAACTTACTCCCGAAGAGATGTCTGAGCTCGAATCCCTTGCACAACCAGGTTTTGTGAAAGGAGAAAGATCCATTAGCATCCTGACCACGTTCAAAAACTCTGAGACTCCACCATTGTCTTCTTGGAAAGCCGCTTAA

Protein Analysis

345

Amino Acids

37.86

Weight (kDa)

5.91

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 26 - 313 3.4e-67 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10810 AT1G10810 AT1G60680 AT1G60690 AT1G60710 AT1G60730 AT1G60730 AT1G60750
fragaria_vesca FvH4_4g12490
malus_domestica MD04G1026100.v1.1 MD09G1032900.v1.1 MD13G1279900.v1.1
prunus_persica Prupe.1G175500_v2.0.a1 Prupe.1G175600_v2.0.a1 Prupe.1G175700_v2.0.a1 Prupe.1G175800_v2.0.a1 Prupe.2G050800_v2.0.a1
pyrus_communis pycom04g02210 pycom04g02230 pycom111g02620 pycom13g29370
rosa_chinensis RchiOBHm_Chr4g0409691 RchiOBHm_Chr4g0410671 RchiOBHm_Chr4g0410821 RchiOBHm_Chr4g0411011 RchiOBHm_Chr4g0411051 RchiOBHm_Chr4g0411101 RchiOBHm_Chr4g0411151 RchiOBHm_Chr4g0411171 RchiOBHm_Chr4g0411201
rosa_laevigata RLG00000008369 RLG00000008372 RLG00000008374 RLG00000008376 RLG00000008378 RLG00000008381 RLG00000008383 RLG00000008395
rosa_multiflora Rmu_co8497103.1_g000001 Rmu_co8514481.1_g000001 Rmu_sc0000543.1_g000005 Rmu_sc0000543.1_g000006 Rmu_sc0000543.1_g000024 Rmu_sc0000944.1_g000002 Rmu_sc0000944.1_g000011 Rmu_sc0001244.1_g000006 Rmu_sc0001244.1_g000014 Rmu_sc0001244.1_g000017 Rmu_sc0001244.1_g000020 Rmu_sc0003150.1_g000001 Rmu_sc0007879.1_g000001 Rmu_sc0007879.1_g000007 Rmu_sc0009499.1_g000008 Rmu_sc0029727.1_g000001
rosa_roxburghii Rroxscaffold_5G00354230 Rroxscaffold_5G00355560 Rroxscaffold_5G00355580 Rroxscaffold_5G00355600 Rroxscaffold_5G00355650 Rroxscaffold_5G00355710 Rroxscaffold_5G00355730 Rroxscaffold_5G00355760 Rroxscaffold_5G00355800 Rroxscaffold_5G00355840 Rroxscaffold_5G00355850
rosa_rugosa Rorug04G0096400 Rorug04G0105400 Rorug04G0110600 Rorug04G0110700 Rorug05G0412600 Rorug07G0180500
rosa_samantha Rh4AG157500 Rh4BG162500 Rh4BG163400 Rh4CG175300 Rh4CG176100 Rh4CG177100 Rh4CG177200 Rh4CG177400 Rh4CG177500 Rh4CG178000 Rh4CG178100 Rh4CG178300 Rh4DG159900 Rh4DG160200
rosa_wichuraiana Rw4G013600 Rw4G013610 Rw4G013660 Rw4G013690 Rw4G013700 Rw4G013710 Rw4G013720 Rw4G013730 Rw4G013740 Rw4G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 693
Acc36I ACCTGC 1 cut(s) 599
AccBSI CCGCTC 1 cut(s) 533
AciI CCGC 4 cut(s) 5, 15, 531, 1032
AclWI GGATC 2 cut(s) 305, 963
AcuI CTGAAG 4 cut(s) 455, 471, 501, 636
AcyI GRCGYC 2 cut(s) 159, 690
AfaI GTAC 1 cut(s) 103
AfiI CCNNNNNNNGG 4 cut(s) 220, 810, 811, 848
AgsI TTSAA 4 cut(s) 449, 495, 866, 994
AjiI CACGTC 1 cut(s) 568
AjnI CCWGG 3 cut(s) 822, 847, 946
AjuI GAANNNNNNNTTGG 4 cut(s) 408, 440, 591, 623
Alw21I GWGCWC 2 cut(s) 511, 930
Alw26I GTCTC 4 cut(s) 89, 191, 558, 998
AlwI GGATC 2 cut(s) 305, 963
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 1 cut(s) 329
AspLEI GCGC 1 cut(s) 64
AspS9I GGNCC 3 cut(s) 191, 549, 656
AsuHPI GGTGA 3 cut(s) 250, 512, 812
AvaII GGWCC 3 cut(s) 191, 549, 656
BaeI ACNNNNGTAYC 2 cut(s) 390, 423
BanII GRGCYC 1 cut(s) 930
BauI CACGAG 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 1011
Bbv12I GWGCWC 2 cut(s) 511, 930
BbvCI CCTCAGC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 316
BccI CCATC 2 cut(s) 227, 851
BcgI CGANNNNNNTGC 2 cut(s) 920, 954
BciT130I CCWGG 3 cut(s) 824, 849, 948
BcoDI GTCTC 4 cut(s) 89, 191, 558, 998
BfaI CTAG 5 cut(s) 35, 342, 443, 677, 800
BfmI CTRYAG 1 cut(s) 592
BfuAI ACCTGC 1 cut(s) 599
BisI GCNGC 2 cut(s) 330, 1032
BlsI GCNGC 2 cut(s) 331, 1033
Bme1390I CCNGG 3 cut(s) 824, 849, 948
Bme18I GGWCC 3 cut(s) 191, 549, 656
BmgBI CACGTC 1 cut(s) 568
BmgT120I GGNCC 3 cut(s) 191, 549, 656
BmiI GGNNCC 2 cut(s) 658, 853
BmrFI CCNGG 3 cut(s) 824, 849, 948
BmsI GCATC 3 cut(s) 397, 773, 987
BpiI GAAGAC 1 cut(s) 1011
Bpu10I CCTNAGC 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 71, 212
BsaHI GRCGYC 2 cut(s) 159, 690
BsaI GGTCTC 2 cut(s) 89, 191
BsaJI CCNNGG 3 cut(s) 43, 823, 847
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 4 cut(s) 220, 810, 811, 848
Bse3DI GCAATG 1 cut(s) 771
BseBI CCWGG 3 cut(s) 824, 849, 948
BseDI CCNNGG 3 cut(s) 43, 823, 847
BseGI GGATG 5 cut(s) 135, 238, 788, 843, 978
BseLI CCNNNNNNNGG 4 cut(s) 220, 810, 811, 848
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 5 cut(s) 186, 229, 762, 915, 993
BseRI GAGGAG 1 cut(s) 203
BseXI GCAGC 1 cut(s) 316
BsgI GTGCAG 1 cut(s) 777
BshFI GGCC 1 cut(s) 48
BsiHKAI GWGCWC 2 cut(s) 511, 930
BsiSI CCGG 2 cut(s) 113, 156
BslFI GGGAC 2 cut(s) 392, 648
BslI CCNNNNNNNGG 4 cut(s) 220, 810, 811, 848
BsmAI GTCTC 4 cut(s) 89, 191, 558, 998
BsmBI CGTCTC 1 cut(s) 558
BsmFI GGGAC 2 cut(s) 392, 648
BsnI GGCC 1 cut(s) 48
Bso31I GGTCTC 2 cut(s) 89, 191
Bsp1286I GDGCHC 2 cut(s) 511, 930
Bsp143I GATC 3 cut(s) 310, 373, 968
BspACI CCGC 4 cut(s) 5, 15, 531, 1032
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 5 cut(s) 187, 228, 763, 916, 994
BspLI GGNNCC 2 cut(s) 658, 853
BspMAI CTGCAG 1 cut(s) 596
BspMI ACCTGC 1 cut(s) 599
BspPI GGATC 2 cut(s) 305, 963
BspQI GCTCTTC 1 cut(s) 499
BspTNI GGTCTC 2 cut(s) 89, 191
BsrBI CCGCTC 1 cut(s) 533
BsrDI GCAATG 1 cut(s) 771
BssECI CCNNGG 3 cut(s) 43, 823, 847
BssMI GATC 3 cut(s) 310, 373, 968
BssNI GRCGYC 2 cut(s) 159, 690
BssSI CACGAG 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 43
Bst2BI CACGAG 1 cut(s) 402
Bst2UI CCWGG 3 cut(s) 824, 849, 948
Bst4CI ACNGT 3 cut(s) 101, 191, 626
Bst6I CTCTTC 2 cut(s) 499, 909
BstACI GRCGYC 2 cut(s) 159, 690
BstC8I GCNNGC 2 cut(s) 13, 367
BstDEI CTNAG 7 cut(s) 195, 215, 727, 771, 858, 924, 1002
BstENI CCTNNNNNAGG 1 cut(s) 218
BstF5I GGATG 5 cut(s) 135, 238, 788, 843, 978
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 3 cut(s) 313, 376, 971
BstMAI GTCTC 4 cut(s) 89, 191, 558, 998
BstMBI GATC 3 cut(s) 310, 373, 968
BstMWI GCNNNNNNNGC 3 cut(s) 338, 439, 800
BstNI CCWGG 3 cut(s) 824, 849, 948
BstNSI RCATGY 1 cut(s) 515
BstSCI CCNGG 3 cut(s) 822, 847, 946
BstSFI CTRYAG 1 cut(s) 592
BstV1I GCAGC 1 cut(s) 316
BstV2I GAAGAC 1 cut(s) 1011
BstX2I RGATCY 2 cut(s) 310, 968
BstXI CCANNNNNNTGG 1 cut(s) 884
BstYI RGATCY 2 cut(s) 310, 968
BsuRI GGCC 1 cut(s) 48
BtrI CACGTC 1 cut(s) 568
BtsCI GGATG 5 cut(s) 135, 238, 788, 843, 978
BtsIMutI CAGTG 1 cut(s) 903
BveI ACCTGC 1 cut(s) 599
Cac8I GCNNGC 2 cut(s) 13, 367
CfoI GCGC 1 cut(s) 64
Cfr13I GGNCC 3 cut(s) 191, 549, 656
CseI GACGC 1 cut(s) 148
CsiI ACCWGGT 1 cut(s) 946
Csp6I GTAC 1 cut(s) 102
CspCI CAANNNNNGTGG 2 cut(s) 844, 879
CviAII CATG 3 cut(s) 79, 512, 810
CviQI GTAC 1 cut(s) 102
DdeI CTNAG 7 cut(s) 195, 215, 727, 771, 858, 924, 1002
DpnI GATC 3 cut(s) 312, 375, 970
DpnII GATC 3 cut(s) 310, 373, 968
Eam1104I CTCTTC 2 cut(s) 499, 909
EarI CTCTTC 2 cut(s) 499, 909
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 928
Eco130I CCWWGG 1 cut(s) 43
Eco147I AGGCCT 1 cut(s) 48
Eco24I GRGCYC 1 cut(s) 930
Eco31I GGTCTC 2 cut(s) 89, 191
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 3 cut(s) 191, 549, 656
Eco53kI GAGCTC 1 cut(s) 928
Eco57I CTGAAG 4 cut(s) 455, 471, 501, 636
EcoICRI GAGCTC 1 cut(s) 928
EcoNI CCTNNNNNAGG 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 656
EcoRII CCWGG 3 cut(s) 822, 847, 946
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 43
EcoT38I GRGCYC 1 cut(s) 930
ErhI CCWWGG 1 cut(s) 43
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 3 cut(s) 82, 515, 813
FaiI YATR 9 cut(s) 80, 270, 282, 321, 468, 513, 556, 581, 811
FalI AAGNNNNNCTT 4 cut(s) 240, 272, 731, 763
FaqI GGGAC 2 cut(s) 392, 648
FatI CATG 3 cut(s) 78, 511, 809
Fnu4HI GCNGC 2 cut(s) 330, 1032
FokI GGATG 5 cut(s) 122, 245, 795, 830, 965
FriOI GRGCYC 1 cut(s) 930
Fsp4HI GCNGC 2 cut(s) 330, 1032
FspBI CTAG 5 cut(s) 35, 342, 443, 677, 800
GlaI GCGC 1 cut(s) 63
GluI GCNGC 2 cut(s) 330, 1032
HaeIII GGCC 1 cut(s) 48
HapII CCGG 2 cut(s) 113, 156
HgaI GACGC 1 cut(s) 148
HhaI GCGC 1 cut(s) 64
Hin1I GRCGYC 2 cut(s) 159, 690
Hin1II CATG 3 cut(s) 82, 515, 813
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HindIII AAGCTT 3 cut(s) 9, 483, 662
HinfI GANTC 6 cut(s) 273, 700, 711, 742, 932, 1006
HpaII CCGG 2 cut(s) 113, 156
HphI GGTGA 3 cut(s) 250, 512, 812
Hpy166II GTNNAC 1 cut(s) 520
Hpy188I TCNGA 7 cut(s) 181, 481, 503, 695, 772, 925, 1003
Hpy188III TCNNGA 5 cut(s) 194, 561, 654, 911, 982
Hpy8I GTNNAC 1 cut(s) 520
HpyAV CCTTC 3 cut(s) 18, 446, 748
HpyCH4III ACNGT 3 cut(s) 101, 191, 626
HpyCH4IV ACGT 3 cut(s) 567, 690, 989
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 439, 800
HpyF3I CTNAG 7 cut(s) 195, 215, 727, 771, 858, 924, 1002
HpySE526I ACGT 3 cut(s) 567, 690, 989
Hsp92I GRCGYC 2 cut(s) 159, 690
Hsp92II CATG 3 cut(s) 82, 515, 813
HspAI GCGC 1 cut(s) 62
Kzo9I GATC 3 cut(s) 310, 373, 968
LguI GCTCTTC 1 cut(s) 499
LmnI GCTCC 1 cut(s) 887
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 3 cut(s) 397, 773, 987
MabI ACCWGGT 1 cut(s) 946
MaeI CTAG 5 cut(s) 35, 342, 443, 677, 800
MaeII ACGT 3 cut(s) 567, 690, 989
MaeIII GTNAC 1 cut(s) 160
MalI GATC 3 cut(s) 312, 375, 970
MbiI CCGCTC 1 cut(s) 533
MboI GATC 3 cut(s) 310, 373, 968
MboII GAAGA 8 cut(s) 448, 461, 516, 584, 587, 787, 926, 1011
MflI RGATCY 2 cut(s) 310, 968
MhlI GDGCHC 2 cut(s) 511, 930
MluCI AATT 3 cut(s) 393, 582, 612
MlyI GAGTC 2 cut(s) 694, 1000
MmeI TCCRAC 2 cut(s) 611, 739
MnlI CCTC 8 cut(s) 46, 187, 224, 280, 331, 632, 639, 881
MseI TTAA 4 cut(s) 30, 303, 347, 1036
MspI CCGG 2 cut(s) 113, 156
MspR9I CCNGG 3 cut(s) 824, 849, 948
MvaI CCWGG 3 cut(s) 824, 849, 948
MwoI GCNNNNNNNGC 3 cut(s) 338, 439, 800
NdeII GATC 3 cut(s) 310, 373, 968
NlaIII CATG 3 cut(s) 82, 515, 813
NlaIV GGNNCC 2 cut(s) 658, 853
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 1 cut(s) 515
PceI AGGCCT 1 cut(s) 48
PciSI GCTCTTC 1 cut(s) 499
PfeI GAWTC 4 cut(s) 273, 711, 742, 932
PkrI GCNGC 2 cut(s) 331, 1033
PleI GAGTC 2 cut(s) 694, 1000
PpsI GAGTC 2 cut(s) 694, 1000
PpuMI RGGWCCY 1 cut(s) 656
Psp124BI GAGCTC 1 cut(s) 930
Psp5II RGGWCCY 1 cut(s) 656
Psp6I CCWGG 3 cut(s) 822, 847, 946
PspGI CCWGG 3 cut(s) 822, 847, 946
PspN4I GGNNCC 2 cut(s) 658, 853
PspPI GGNCC 3 cut(s) 191, 549, 656
PspPPI RGGWCCY 1 cut(s) 656
PstI CTGCAG 1 cut(s) 596
PsuI RGATCY 2 cut(s) 310, 968
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
SacI GAGCTC 1 cut(s) 930
SapI GCTCTTC 1 cut(s) 499
SaqAI TTAA 4 cut(s) 30, 303, 347, 1036
SatI GCNGC 2 cut(s) 330, 1032
Sau3AI GATC 3 cut(s) 310, 373, 968
Sau96I GGNCC 3 cut(s) 191, 549, 656
SbfI CCTGCAGG 1 cut(s) 596
SchI GAGTC 2 cut(s) 694, 1000
ScrFI CCNGG 3 cut(s) 824, 849, 948
SdaI CCTGCAGG 1 cut(s) 596
SduI GDGCHC 2 cut(s) 511, 930
SexAI ACCWGGT 1 cut(s) 946
SfaNI GCATC 3 cut(s) 397, 773, 987
SfcI CTRYAG 1 cut(s) 592
SinI GGWCC 3 cut(s) 191, 549, 656
SmlI CTYRAG 2 cut(s) 50, 227
SmoI CTYRAG 2 cut(s) 50, 227
Sse8387I CCTGCAGG 1 cut(s) 596
Sse9I AATT 3 cut(s) 393, 582, 612
SseBI AGGCCT 1 cut(s) 48
SsiI CCGC 4 cut(s) 5, 15, 531, 1032
SspMI CTAG 5 cut(s) 35, 342, 443, 677, 800
SstI GAGCTC 1 cut(s) 930
StuI AGGCCT 1 cut(s) 48
StyD4I CCNGG 3 cut(s) 822, 847, 946
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 3 cut(s) 101, 191, 626
TaiI ACGT 3 cut(s) 570, 693, 992
TaqI TCGA 4 cut(s) 391, 414, 560, 930
TasI AATT 3 cut(s) 393, 582, 612
TauI GCSGC 1 cut(s) 1034
TfiI GAWTC 4 cut(s) 273, 711, 742, 932
Tru1I TTAA 4 cut(s) 30, 303, 347, 1036
Tru9I TTAA 4 cut(s) 30, 303, 347, 1036
TscAI CASTG 1 cut(s) 903
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 1 cut(s) 329
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 1 cut(s) 806
TspRI CASTG 1 cut(s) 903
VpaK11BI GGWCC 3 cut(s) 191, 549, 656
XagI CCTNNNNNAGG 1 cut(s) 218
XceI RCATGY 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 1020
XspI CTAG 5 cut(s) 35, 342, 443, 677, 800
ZraI GACGTC 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.