Rw4G013730

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000435
Physical Location & Seq
Forward (+)
32125691 .. 32128784
3094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013730.1

Sequence Viewer

Length: 1017 bp
ATGGCGGGTGTGAGGAGGATGAAGCTCGGGTCACAAGGCCTGGAGGTCTCAGCCCAAGGACTGGGCTGCATGGGCATGTCAGCCTTCTATGGCCCCCCGAAGCCCGAAGCTGACATGATCAAGCTCATCCACCATGCTGTCGACTCCGGCGTCACCTTCCTCGACACTTCCGACGTCTACGGTCCCCACACCAACGAAATTCTCCTCGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAACTGGCCACCAAATGCGGTATCAGGTTTGCGGACAACAAAATGGAGGTTCAAGGCGACCCGGCATATGTGAGAGCTGCTTGTGAAGGAAGCTTGAAACGCCTTGGTGTTGCTTCTGTTGATCTCTATTATCAGCATAGGATTGACACCCGTGTCCCCATTGAAGTCACGGTGGGGGAGCTCAAGAAGCTAATTGAAGAGGGTAAAATAAAGTACATCGGTCTGTCTGAGGCCTCAGCTTCCACAATAAGAAGAGCCCATGCTGTTCATCCAATAACTGCTGTGCAGCTGGAGTGGTCCTTGTGGTCAAGAGATGTAGAAGAAGAAATAATCCCTACTTGTCGGGAACTTGGCATTGGCATTGTTGCATACAGTCCTCTAGGAAGAGGATTCTTATCATCGGGGGCAAATATCATCGAAAATCTTTCCAATGATGATTCTCGAAAGGATATGCCCAGGTTCCAAGCTGAAAACCTAGAGCATAATAAAACAATATTTGAGCGAGTAAGTGATCTTGCAGCAAGGAAGGGTTGCACCCCATCTCAACTAGCACTGGCCTGGGTTCATCACCAAGGAAATGATGTGTGCCCTATACCCGGAACCACCAAGATTGAGAATTTTAACGAGAACATTAAGGCTCTGTCTGTGAAACTGACACCAGAAGAAATGGCCGAGCTTGAATCTTTTGCTTCCGCAGACGCTGTTAAGGGCGACAGATATCAGTCTGGCTTCAGCACTTGGATGAACTCTGAAACTCCACCACTGTTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

338

Amino Acids

36.96

Weight (kDa)

5.78

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 310 9.4e-74 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10810 AT1G10810 AT1G60680 AT1G60690 AT1G60710 AT1G60730 AT1G60730 AT1G60750
fragaria_vesca FvH4_4g12490
malus_domestica MD04G1026100.v1.1 MD09G1032900.v1.1 MD13G1279900.v1.1
prunus_persica Prupe.1G175500_v2.0.a1 Prupe.1G175600_v2.0.a1 Prupe.1G175700_v2.0.a1 Prupe.1G175800_v2.0.a1 Prupe.2G050800_v2.0.a1
pyrus_communis pycom04g02210 pycom04g02230 pycom111g02620 pycom13g29370
rosa_chinensis RchiOBHm_Chr4g0409691 RchiOBHm_Chr4g0410671 RchiOBHm_Chr4g0410821 RchiOBHm_Chr4g0411011 RchiOBHm_Chr4g0411051 RchiOBHm_Chr4g0411101 RchiOBHm_Chr4g0411151 RchiOBHm_Chr4g0411171 RchiOBHm_Chr4g0411201
rosa_laevigata RLG00000008369 RLG00000008372 RLG00000008374 RLG00000008376 RLG00000008378 RLG00000008381 RLG00000008383 RLG00000008395
rosa_multiflora Rmu_co8497103.1_g000001 Rmu_co8514481.1_g000001 Rmu_sc0000543.1_g000005 Rmu_sc0000543.1_g000006 Rmu_sc0000543.1_g000024 Rmu_sc0000944.1_g000002 Rmu_sc0000944.1_g000011 Rmu_sc0001244.1_g000006 Rmu_sc0001244.1_g000014 Rmu_sc0001244.1_g000017 Rmu_sc0001244.1_g000020 Rmu_sc0003150.1_g000001 Rmu_sc0007879.1_g000001 Rmu_sc0007879.1_g000007 Rmu_sc0009499.1_g000008 Rmu_sc0029727.1_g000001
rosa_roxburghii Rroxscaffold_5G00354230 Rroxscaffold_5G00355560 Rroxscaffold_5G00355580 Rroxscaffold_5G00355600 Rroxscaffold_5G00355650 Rroxscaffold_5G00355710 Rroxscaffold_5G00355730 Rroxscaffold_5G00355760 Rroxscaffold_5G00355800 Rroxscaffold_5G00355840 Rroxscaffold_5G00355850
rosa_rugosa Rorug04G0096400 Rorug04G0105400 Rorug04G0110600 Rorug04G0110700 Rorug05G0412600 Rorug07G0180500
rosa_samantha Rh4AG157500 Rh4BG162500 Rh4BG163400 Rh4CG175300 Rh4CG176100 Rh4CG177100 Rh4CG177200 Rh4CG177400 Rh4CG177500 Rh4CG178000 Rh4CG178100 Rh4CG178300 Rh4DG159900 Rh4DG160200
rosa_wichuraiana Rw4G013600 Rw4G013610 Rw4G013660 Rw4G013690 Rw4G013700 Rw4G013710 Rw4G013720 Rw4G013730 Rw4G013740 Rw4G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 149, 395
AatII GACGTC 1 cut(s) 177
AccB7I CCANNNNNTGG 1 cut(s) 61
AccI GTMKAC 2 cut(s) 141, 177
AciI CCGC 4 cut(s) 5, 261, 275, 936
AcoI YGGCCR 2 cut(s) 249, 912
AcsI RAATTY 2 cut(s) 198, 859
AcuI CTGAAG 1 cut(s) 958
AcyI GRCGYC 2 cut(s) 150, 174
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 3 cut(s) 61, 211, 839
AgsI TTSAA 6 cut(s) 245, 296, 340, 407, 440, 923
AjnI CCWGG 3 cut(s) 39, 698, 800
AjuI GAANNNNNNNTTGG 2 cut(s) 582, 614
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 2 cut(s) 52, 228
AlwNI CAGNNNCTG 1 cut(s) 944
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 6 cut(s) 37, 91, 249, 474, 798, 912
ApeKI GCWGC 4 cut(s) 66, 320, 529, 761
ApoI RAATTY 2 cut(s) 198, 859
AspS9I GGNCC 3 cut(s) 92, 182, 540
AsuC2I CCSGG 2 cut(s) 305, 840
AsuHPI GGTGA 3 cut(s) 145, 241, 803
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 2 cut(s) 182, 540
BaeGI GKGCMC 1 cut(s) 833
BalI TGGCCA 1 cut(s) 251
BanII GRGCYC 2 cut(s) 426, 502
Bbv12I GWGCWC 1 cut(s) 426
BbvCI CCTCAGC 1 cut(s) 478
BbvI GCAGC 4 cut(s) 53, 307, 541, 773
BccI CCATC 1 cut(s) 790
BciT130I CCWGG 3 cut(s) 41, 700, 802
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 2 cut(s) 305, 840
BcoDI GTCTC 2 cut(s) 52, 228
BfaI CTAG 3 cut(s) 623, 719, 791
BisI GCNGC 4 cut(s) 67, 321, 530, 762
BlsI GCNGC 4 cut(s) 68, 322, 531, 763
Bme1390I CCNGG 5 cut(s) 41, 305, 700, 802, 840
Bme18I GGWCC 2 cut(s) 182, 540
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 3 cut(s) 92, 182, 540
BmiI GGNNCC 4 cut(s) 94, 184, 704, 844
BmrFI CCNGG 5 cut(s) 41, 305, 700, 802, 840
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 2 cut(s) 62, 554
Bpu10I CCTNAGC 1 cut(s) 478
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 2 cut(s) 305, 840
BsaBI GATNNNNATC 1 cut(s) 637
BsaHI GRCGYC 2 cut(s) 150, 174
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 6 cut(s) 55, 205, 346, 698, 801, 814
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 211, 839
Bse1I ACTGG 3 cut(s) 66, 252, 801
Bse8I GATNNNNATC 1 cut(s) 637
BseBI CCWGG 3 cut(s) 41, 700, 802
BseDI CCNNGG 6 cut(s) 55, 205, 346, 698, 801, 814
BseGI GGATG 4 cut(s) 24, 126, 511, 990
BseJI GATNNNNATC 1 cut(s) 637
BseLI CCNNNNNNNGG 3 cut(s) 61, 211, 839
BseMII CTCAG 3 cut(s) 63, 462, 492
BseNI ACTGG 3 cut(s) 66, 252, 801
BseRI GAGGAG 2 cut(s) 28, 194
BseSI GKGCMC 1 cut(s) 833
BseXI GCAGC 4 cut(s) 53, 307, 541, 773
BsgI GTGCAG 1 cut(s) 548
BshFI GGCC 6 cut(s) 39, 93, 251, 476, 800, 914
BsiHKAI GWGCWC 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 3 cut(s) 147, 305, 840
BslFI GGGAC 2 cut(s) 168, 383
BslI CCNNNNNNNGG 3 cut(s) 61, 211, 839
BsmAI GTCTC 2 cut(s) 52, 228
BsmFI GGGAC 2 cut(s) 168, 383
BsnI GGCC 6 cut(s) 39, 93, 251, 476, 800, 914
Bso31I GGTCTC 1 cut(s) 52
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 3 cut(s) 426, 502, 833
Bsp143I GATC 3 cut(s) 117, 364, 754
BspACI CCGC 4 cut(s) 5, 261, 275, 936
BspANI GGCC 6 cut(s) 39, 93, 251, 476, 800, 914
BspCNI CTCAG 3 cut(s) 62, 463, 491
BspHI TCATGA 1 cut(s) 1013
BspLI GGNNCC 4 cut(s) 94, 184, 704, 844
BspQI GCTCTTC 1 cut(s) 490
BspTNI GGTCTC 1 cut(s) 52
BsrI ACTGG 3 cut(s) 66, 252, 801
BssECI CCNNGG 6 cut(s) 55, 205, 346, 698, 801, 814
BssMI GATC 3 cut(s) 117, 364, 754
BssNI GRCGYC 2 cut(s) 150, 174
BssT1I CCWWGG 3 cut(s) 55, 346, 814
Bst2UI CCWGG 3 cut(s) 41, 700, 802
Bst4CI ACNGT 4 cut(s) 182, 415, 617, 1008
Bst6I CTCTTC 3 cut(s) 435, 490, 622
BstACI GRCGYC 2 cut(s) 150, 174
BstDEI CTNAG 3 cut(s) 49, 471, 478
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 4 cut(s) 24, 126, 511, 990
BstKTI GATC 3 cut(s) 120, 367, 757
BstMAI GTCTC 2 cut(s) 52, 228
BstMBI GATC 3 cut(s) 117, 364, 754
BstMWI GCNNNNNNNGC 3 cut(s) 72, 342, 430
BstNI CCWGG 3 cut(s) 41, 700, 802
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 5 cut(s) 39, 303, 698, 800, 838
BstSLI GKGCMC 1 cut(s) 833
BstV1I GCAGC 4 cut(s) 53, 307, 541, 773
BsuRI GGCC 6 cut(s) 39, 93, 251, 476, 800, 914
BtsCI GGATG 4 cut(s) 24, 126, 511, 990
BtsIMutI CAGTG 2 cut(s) 794, 1004
CaiI CAGNNNCTG 1 cut(s) 944
CciI TCATGA 1 cut(s) 1013
Cfr13I GGNCC 3 cut(s) 92, 182, 540
CseI GACGC 2 cut(s) 139, 950
Csp6I GTAC 1 cut(s) 457
CspCI CAANNNNNGTGG 2 cut(s) 835, 870
CviAII CATG 6 cut(s) 70, 76, 115, 134, 503, 1014
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 3 cut(s) 49, 471, 478
DpnI GATC 3 cut(s) 119, 366, 756
DpnII GATC 3 cut(s) 117, 364, 754
DrdI GACNNNNNNGTC 2 cut(s) 149, 395
DseDI GACNNNNNNGTC 2 cut(s) 149, 395
EaeI YGGCCR 2 cut(s) 249, 912
Eam1104I CTCTTC 3 cut(s) 435, 490, 622
EarI CTCTTC 3 cut(s) 435, 490, 622
Ecl136II GAGCTC 1 cut(s) 424
Eco130I CCWWGG 3 cut(s) 55, 346, 814
Eco147I AGGCCT 2 cut(s) 39, 476
Eco24I GRGCYC 2 cut(s) 426, 502
Eco31I GGTCTC 1 cut(s) 52
Eco32I GATATC 1 cut(s) 962
Eco47I GGWCC 2 cut(s) 182, 540
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 958
Eco88I CYCGRG 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 424
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 3 cut(s) 39, 698, 800
EcoRV GATATC 1 cut(s) 962
EcoT14I CCWWGG 3 cut(s) 55, 346, 814
EcoT38I GRGCYC 2 cut(s) 426, 502
ErhI CCWWGG 3 cut(s) 55, 346, 814
FaeI CATG 6 cut(s) 73, 79, 118, 137, 506, 1017
FaqI GGGAC 2 cut(s) 168, 383
FatI CATG 6 cut(s) 69, 75, 114, 133, 502, 1013
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 2 cut(s) 141, 177
Fnu4HI GCNGC 4 cut(s) 67, 321, 530, 762
FokI GGATG 4 cut(s) 31, 113, 498, 997
FriOI GRGCYC 2 cut(s) 426, 502
Fsp4HI GCNGC 4 cut(s) 67, 321, 530, 762
FspBI CTAG 3 cut(s) 623, 719, 791
GluI GCNGC 4 cut(s) 67, 321, 530, 762
GsuI CTGGAG 2 cut(s) 62, 554
HaeIII GGCC 6 cut(s) 39, 93, 251, 476, 800, 914
HapII CCGG 3 cut(s) 147, 305, 840
HgaI GACGC 2 cut(s) 139, 950
Hin1I GRCGYC 2 cut(s) 150, 174
Hin1II CATG 6 cut(s) 73, 79, 118, 137, 506, 1017
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 4 cut(s) 143, 633, 680, 923
HpaII CCGG 3 cut(s) 147, 305, 840
HphI GGTGA 3 cut(s) 145, 241, 803
Hpy166II GTNNAC 2 cut(s) 142, 178
Hpy188I TCNGA 3 cut(s) 172, 472, 994
Hpy188III TCNNGA 5 cut(s) 427, 552, 587, 684, 1014
Hpy8I GTNNAC 2 cut(s) 142, 178
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 4 cut(s) 94, 166, 323, 763
HpyCH4III ACNGT 4 cut(s) 182, 415, 617, 1008
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 5 cut(s) 69, 529, 611, 761, 777
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 342, 430
HpyF3I CTNAG 3 cut(s) 49, 471, 478
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 2 cut(s) 150, 174
Hsp92II CATG 6 cut(s) 73, 79, 118, 137, 506, 1017
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 117, 364, 754
LguI GCTCTTC 1 cut(s) 490
LmnI GCTCC 1 cut(s) 421
Lsp1109I GCAGC 4 cut(s) 53, 307, 541, 773
MaeI CTAG 3 cut(s) 623, 719, 791
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 3 cut(s) 30, 151, 409
MalI GATC 3 cut(s) 119, 366, 756
MboI GATC 3 cut(s) 117, 364, 754
MboII GAAGA 6 cut(s) 452, 507, 575, 578, 639, 917
MhlI GDGCHC 3 cut(s) 426, 502, 833
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 3 cut(s) 198, 435, 859
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MseI TTAA 3 cut(s) 864, 876, 948
MslI CAYNNNNRTG 2 cut(s) 74, 983
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 532
MspI CCGG 3 cut(s) 147, 305, 840
MspR9I CCNGG 5 cut(s) 41, 305, 700, 802, 840
MvaI CCWGG 3 cut(s) 41, 700, 802
MwoI GCNNNNNNNGC 3 cut(s) 72, 342, 430
NciI CCSGG 2 cut(s) 305, 840
NdeI CATATG 1 cut(s) 310
NdeII GATC 3 cut(s) 117, 364, 754
NlaIII CATG 6 cut(s) 73, 79, 118, 137, 506, 1017
NlaIV GGNNCC 4 cut(s) 94, 184, 704, 844
NmeAIII GCCGAG 2 cut(s) 186, 940
NmuCI GTSAC 3 cut(s) 30, 151, 409
NspI RCATGY 1 cut(s) 79
PagI TCATGA 1 cut(s) 1013
PceI AGGCCT 2 cut(s) 39, 476
PciSI GCTCTTC 1 cut(s) 490
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PfeI GAWTC 3 cut(s) 633, 680, 923
PflMI CCANNNNNTGG 1 cut(s) 61
PkrI GCNGC 4 cut(s) 68, 322, 531, 763
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 426
Psp6I CCWGG 3 cut(s) 39, 698, 800
PspGI CCWGG 3 cut(s) 39, 698, 800
PspN4I GGNNCC 4 cut(s) 94, 184, 704, 844
PspPI GGNCC 3 cut(s) 92, 182, 540
PstNI CAGNNNCTG 1 cut(s) 944
PvuII CAGCTG 1 cut(s) 532
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
RseI CAYNNNNRTG 2 cut(s) 74, 983
SacI GAGCTC 1 cut(s) 426
SalI GTCGAC 1 cut(s) 140
SapI GCTCTTC 1 cut(s) 490
SaqAI TTAA 3 cut(s) 864, 876, 948
SatI GCNGC 4 cut(s) 67, 321, 530, 762
Sau3AI GATC 3 cut(s) 117, 364, 754
Sau96I GGNCC 3 cut(s) 92, 182, 540
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 5 cut(s) 41, 305, 700, 802, 840
SduI GDGCHC 3 cut(s) 426, 502, 833
SinI GGWCC 2 cut(s) 182, 540
SmiMI CAYNNNNRTG 2 cut(s) 74, 983
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 3 cut(s) 198, 435, 859
SseBI AGGCCT 2 cut(s) 39, 476
SsiI CCGC 4 cut(s) 5, 261, 275, 936
SspI AATATT 1 cut(s) 738
SspMI CTAG 3 cut(s) 623, 719, 791
SstI GAGCTC 1 cut(s) 426
StuI AGGCCT 2 cut(s) 39, 476
StyD4I CCNGG 5 cut(s) 39, 303, 698, 800, 838
StyI CCWWGG 3 cut(s) 55, 346, 814
TaaI ACNGT 4 cut(s) 182, 415, 617, 1008
TaiI ACGT 1 cut(s) 177
TaqI TCGA 4 cut(s) 141, 162, 660, 685
TaqII GACCGA 1 cut(s) 452
TasI AATT 3 cut(s) 198, 435, 859
TatI WGTACW 1 cut(s) 456
TfiI GAWTC 3 cut(s) 633, 680, 923
Tru1I TTAA 3 cut(s) 864, 876, 948
Tru9I TTAA 3 cut(s) 864, 876, 948
TscAI CASTG 2 cut(s) 801, 1011
TseFI GTSAC 3 cut(s) 30, 151, 409
TseI GCWGC 4 cut(s) 66, 320, 529, 761
Tsp45I GTSAC 3 cut(s) 30, 151, 409
TspDTI ATGAA 5 cut(s) 35, 500, 797, 1001, 1002
TspRI CASTG 2 cut(s) 801, 1011
Van91I CCANNNNNTGG 1 cut(s) 61
VpaK11BI GGWCC 2 cut(s) 182, 540
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 2 cut(s) 198, 859
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 2 cut(s) 141, 177
XspI CTAG 3 cut(s) 623, 719, 791
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.