Rroxscaffold_5G00355600

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000435
Physical Location & Seq
Forward (+)
34338607 .. 34339119
513 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_5G00355600.1

Sequence Viewer

Length: 384 bp
ATGGCGGGCGTGAGGAGGATCAAGCTCGGGTCACAGGGCCTGGAGTATCTACCCAGGTTCCAAGCTGAAAACCTAGAGCATAATCAAACAATATTTGAGCGGGTTAGTGATCTTGCAGCGAGAAAGGGGTGCACCCCATCTCAACTAGCACTAGCTTGGGTTCATCACCAAGGAAATGATGTGTGCCCCATACCCGGAACTACCAAGATTGAGAATTTTAACCAGAATATTGGAGCTTTGTCTGTGAAACTGACACCAGAGGAATTGGCTGAGCTTGAATCCTTTGCTTCTGCGGATGCTGTTAAGGGTGGCCGGTATCAGAATGACTTAAGTACTTGGAAGAACTCCGAAACTCCACCACTGTCTTCATGGAAGGCCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

127

Amino Acids

13.99

Weight (kDa)

6.28

Isoelectric Point (pI)

31.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 24 - 95 1.3e-12 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10810 AT1G10810 AT1G60680 AT1G60690 AT1G60710 AT1G60730 AT1G60730 AT1G60750
fragaria_vesca FvH4_4g12490
malus_domestica MD04G1026100.v1.1 MD09G1032900.v1.1 MD13G1279900.v1.1
prunus_persica Prupe.1G175500_v2.0.a1 Prupe.1G175600_v2.0.a1 Prupe.1G175700_v2.0.a1 Prupe.1G175800_v2.0.a1 Prupe.2G050800_v2.0.a1
pyrus_communis pycom04g02210 pycom04g02230 pycom111g02620 pycom13g29370
rosa_chinensis RchiOBHm_Chr4g0409691 RchiOBHm_Chr4g0410671 RchiOBHm_Chr4g0410821 RchiOBHm_Chr4g0411011 RchiOBHm_Chr4g0411051 RchiOBHm_Chr4g0411101 RchiOBHm_Chr4g0411151 RchiOBHm_Chr4g0411171 RchiOBHm_Chr4g0411201
rosa_laevigata RLG00000008369 RLG00000008372 RLG00000008374 RLG00000008376 RLG00000008378 RLG00000008381 RLG00000008383 RLG00000008395
rosa_multiflora Rmu_co8497103.1_g000001 Rmu_co8514481.1_g000001 Rmu_sc0000543.1_g000005 Rmu_sc0000543.1_g000006 Rmu_sc0000543.1_g000024 Rmu_sc0000944.1_g000002 Rmu_sc0000944.1_g000011 Rmu_sc0001244.1_g000006 Rmu_sc0001244.1_g000014 Rmu_sc0001244.1_g000017 Rmu_sc0001244.1_g000020 Rmu_sc0003150.1_g000001 Rmu_sc0007879.1_g000001 Rmu_sc0007879.1_g000007 Rmu_sc0009499.1_g000008 Rmu_sc0029727.1_g000001
rosa_roxburghii Rroxscaffold_5G00354230 Rroxscaffold_5G00355560 Rroxscaffold_5G00355580 Rroxscaffold_5G00355600 Rroxscaffold_5G00355650 Rroxscaffold_5G00355710 Rroxscaffold_5G00355730 Rroxscaffold_5G00355760 Rroxscaffold_5G00355800 Rroxscaffold_5G00355840 Rroxscaffold_5G00355850
rosa_rugosa Rorug04G0096400 Rorug04G0105400 Rorug04G0110600 Rorug04G0110700 Rorug05G0412600 Rorug07G0180500
rosa_samantha Rh4AG157500 Rh4BG162500 Rh4BG163400 Rh4CG175300 Rh4CG176100 Rh4CG177100 Rh4CG177200 Rh4CG177400 Rh4CG177500 Rh4CG178000 Rh4CG178100 Rh4CG178300 Rh4DG159900 Rh4DG160200
rosa_wichuraiana Rw4G013600 Rw4G013610 Rw4G013660 Rw4G013690 Rw4G013700 Rw4G013710 Rw4G013720 Rw4G013730 Rw4G013740 Rw4G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 100
AciI CCGC 3 cut(s) 5, 100, 293
AclWI GGATC 1 cut(s) 26
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 1 cut(s) 214
AfaI GTAC 1 cut(s) 334
AfiI CCNNNNNNNGG 1 cut(s) 194
AflII CTTAAG 1 cut(s) 328
AgsI TTSAA 1 cut(s) 278
AjnI CCWGG 2 cut(s) 39, 53
AluBI AGCT 5 cut(s) 25, 65, 155, 236, 274
AluI AGCT 5 cut(s) 25, 65, 155, 236, 274
Alw21I GWGCWC 1 cut(s) 134
Alw44I GTGCAC 1 cut(s) 130
AlwI GGATC 1 cut(s) 26
AlwNI CAGNNNCTG 1 cut(s) 40
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 3 cut(s) 37, 310, 375
ApaLI GTGCAC 1 cut(s) 130
ApeKI GCWGC 1 cut(s) 116
ApoI RAATTY 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 26
BaeGI GKGCMC 2 cut(s) 134, 188
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 1 cut(s) 128
BccI CCATC 1 cut(s) 145
BciT130I CCWGG 2 cut(s) 41, 55
BcnI CCSGG 1 cut(s) 195
BfaI CTAG 3 cut(s) 74, 146, 152
BfrI CTTAAG 1 cut(s) 328
BisI GCNGC 1 cut(s) 117
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 118
BmcAI AGTACT 1 cut(s) 334
Bme1390I CCNGG 3 cut(s) 41, 55, 195
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 59
BmrFI CCNGG 3 cut(s) 41, 55, 195
BmsI GCATC 1 cut(s) 286
BpiI GAAGAC 1 cut(s) 357
BpmI CTGGAG 1 cut(s) 62
Bpu1102I GCTNAGC 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 195
BsaJI CCNNGG 2 cut(s) 53, 169
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 1 cut(s) 312
BseBI CCWGG 2 cut(s) 41, 55
BseDI CCNNGG 2 cut(s) 53, 169
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMII CTCAG 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 28
BseSI GKGCMC 2 cut(s) 134, 188
BseXI GCAGC 1 cut(s) 128
BshFI GGCC 3 cut(s) 39, 312, 377
BsiHKAI GWGCWC 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 2 cut(s) 195, 313
BslI CCNNNNNNNGG 1 cut(s) 194
BsnI GGCC 3 cut(s) 39, 312, 377
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 134, 188
Bsp143I GATC 2 cut(s) 18, 109
Bsp1720I GCTNAGC 1 cut(s) 270
BspACI CCGC 3 cut(s) 5, 100, 293
BspANI GGCC 3 cut(s) 39, 312, 377
BspCNI CTCAG 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 59
BspPI GGATC 1 cut(s) 26
BspTI CTTAAG 1 cut(s) 328
BsrBI CCGCTC 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 312
BssAI RCCGGY 1 cut(s) 312
BssECI CCNNGG 2 cut(s) 53, 169
BssMI GATC 2 cut(s) 18, 109
BssT1I CCWWGG 1 cut(s) 169
Bst2UI CCWGG 2 cut(s) 41, 55
Bst4CI ACNGT 1 cut(s) 363
BstAFI CTTAAG 1 cut(s) 328
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 270
BstF5I GGATG 1 cut(s) 301
BstKTI GATC 2 cut(s) 21, 112
BstMBI GATC 2 cut(s) 18, 109
BstNI CCWGG 2 cut(s) 41, 55
BstSCI CCNGG 3 cut(s) 39, 53, 193
BstSLI GKGCMC 2 cut(s) 134, 188
BstV1I GCAGC 1 cut(s) 128
BstV2I GAAGAC 1 cut(s) 357
BstXI CCANNNNNNTGG 1 cut(s) 230
BsuRI GGCC 3 cut(s) 39, 312, 377
BtsCI GGATG 1 cut(s) 301
BtsIMutI CAGTG 1 cut(s) 359
Cac8I GCNNGC 1 cut(s) 7
CaiI CAGNNNCTG 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 312
Cfr13I GGNCC 1 cut(s) 37
Csp6I GTAC 1 cut(s) 333
CviAII CATG 1 cut(s) 369
CviJI RGCY 9 cut(s) 25, 39, 65, 155, 236, 269, 274, 312, 377
CviKI_1 RGCY 9 cut(s) 25, 39, 65, 155, 236, 269, 274, 312, 377
CviQI GTAC 1 cut(s) 333
DdeI CTNAG 1 cut(s) 270
DpnI GATC 2 cut(s) 20, 111
DpnII GATC 2 cut(s) 18, 109
EaeI YGGCCR 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 169
Eco88I CYCGRG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 37
EcoRII CCWGG 2 cut(s) 39, 53
EcoT14I CCWWGG 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 1 cut(s) 372
FaiI YATR 3 cut(s) 81, 191, 370
FatI CATG 1 cut(s) 368
FauI CCCGC 1 cut(s) 93
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 1 cut(s) 117
FspBI CTAG 3 cut(s) 74, 146, 152
GluI GCNGC 1 cut(s) 117
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 39, 312, 377
HapII CCGG 2 cut(s) 195, 313
Hin1II CATG 1 cut(s) 372
HinfI GANTC 1 cut(s) 278
HpaII CCGG 2 cut(s) 195, 313
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 2 cut(s) 321, 349
Hpy8I GTNNAC 1 cut(s) 132
HpyAV CCTTC 1 cut(s) 367
HpyCH4III ACNGT 1 cut(s) 363
HpyCH4V TGCA 2 cut(s) 116, 132
HpyF3I CTNAG 1 cut(s) 270
Hsp92II CATG 1 cut(s) 372
Kzo9I GATC 2 cut(s) 18, 109
LmnI GCTCC 1 cut(s) 233
LpnPI CCDG 9 cut(s) 20, 26, 40, 53, 67, 208, 236, 270, 326
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 1 cut(s) 286
MaeI CTAG 3 cut(s) 74, 146, 152
MaeIII GTNAC 1 cut(s) 30
MalI GATC 2 cut(s) 20, 111
MbiI CCGCTC 1 cut(s) 100
MboI GATC 2 cut(s) 18, 109
MboII GAAGA 2 cut(s) 352, 357
MhlI GDGCHC 2 cut(s) 134, 188
MluCI AATT 2 cut(s) 214, 263
MnlI CCTC 3 cut(s) 6, 9, 253
MseI TTAA 3 cut(s) 219, 303, 329
MspCI CTTAAG 1 cut(s) 328
MspI CCGG 2 cut(s) 195, 313
MspR9I CCNGG 3 cut(s) 41, 55, 195
MvaI CCWGG 2 cut(s) 41, 55
NciI CCSGG 1 cut(s) 195
NdeII GATC 2 cut(s) 18, 109
NlaIII CATG 1 cut(s) 372
NlaIV GGNNCC 1 cut(s) 59
NmuCI GTSAC 1 cut(s) 30
PfeI GAWTC 1 cut(s) 278
PkrI GCNGC 1 cut(s) 118
Psp6I CCWGG 2 cut(s) 39, 53
PspGI CCWGG 2 cut(s) 39, 53
PspN4I GGNNCC 1 cut(s) 59
PspPI GGNCC 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 40
RsaI GTAC 1 cut(s) 334
RsaNI GTAC 1 cut(s) 333
SaqAI TTAA 3 cut(s) 219, 303, 329
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 2 cut(s) 18, 109
Sau96I GGNCC 1 cut(s) 37
ScaI AGTACT 1 cut(s) 334
ScrFI CCNGG 3 cut(s) 41, 55, 195
SduI GDGCHC 2 cut(s) 134, 188
SetI ASST 8 cut(s) 27, 59, 67, 75, 157, 238, 276, 383
SfaNI GCATC 1 cut(s) 286
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
Sse9I AATT 2 cut(s) 214, 263
SsiI CCGC 3 cut(s) 5, 100, 293
SspI AATATT 2 cut(s) 93, 229
SspMI CTAG 3 cut(s) 74, 146, 152
StyD4I CCNGG 3 cut(s) 39, 53, 193
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 1 cut(s) 363
TasI AATT 2 cut(s) 214, 263
TatI WGTACW 1 cut(s) 332
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 3 cut(s) 219, 303, 329
Tru9I TTAA 3 cut(s) 219, 303, 329
TscAI CASTG 1 cut(s) 366
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 2 cut(s) 152, 357
TspRI CASTG 1 cut(s) 366
Vha464I CTTAAG 1 cut(s) 328
VneI GTGCAC 1 cut(s) 130
XapI RAATTY 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 1 cut(s) 366
XspI CTAG 3 cut(s) 74, 146, 152
ZrmI AGTACT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.