FvH4_4g12490

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000435
Physical Location & Seq
Forward (+)
16157332 .. 16161631
4300 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12490.t1

Sequence Viewer

Length: 1029 bp
ATGGCGGGAGTGAGGAGGATCAAGCTGGGTTCGCAAGGCCTGGAGGTCTCGGCTCAAGGACTAGGCTGCATGGGGATGTCAGCCTTCTACGGCCCCCCGAAGCCAGAGCCAGACATGATCAAGCTCATCCACCACGCCGTCGACTCCGGCGTCACCTTCCTCGACACCTCCGACATCTACGGCCCCCACACCAACGAGATTCTCCTCGGCAAGGCTTTGAGCGGAGGAGTGAGGGACAAGGTGGAATTGGCCACCAAATTCGGGATCAGCTTTGCAGACAACAAGAGGGAGGTTCGGGGAGACCCGGCTTATGTGAGGGCTGCTTGTGAAGCAAGCTTGAAACGCCTTGGACTCAATTGTATTGATCTCTATTATCAGCATCGGATCGACACTCGTCTCCCCATTGAAGTCACGGTGGGGGAGCTCAAGAAACTAGTTGAAGAAGGTAAGATAAAGTACATTGGTCTCTCTGAGGCTTCTGCTTCCACAATAAGGAGAGCCCATGCTGTTCATCCAATAACAGCTGTACAGCTGGAGTGGTCCTTGTGGTCAAGAGATGTTGAGGAAGATATTATTCCTACTTGTCGTGAACTTGGCATCGGTATTGTTGCATACAGTCCTCTAGGAAGAGGATTCCTGTCATCTGGGGCTAAGTTCGTGGAAAATCTTGCCAACGATGATTTCCGAAAGTTTCTGCCCAGGTTCCAAGGTGAAAATCTAGAGCACAATAAATCAATATTCGAGCGGGTTAGTGATCTTGCAACAAGGAAGGGGTGCACCTCATCTCAGTTAGCTCTGGCTTGGGTTCATCACCAAGGAAATGATGTGTGCCCCATACCGGGAACCACCAAGATTGAAAATTTTAATCAAAATATTGGAGCTCTGTCTGTGAAACTGACACCAGAGGAAATGGCTGAGCTTGAATCCTTTGCTACTGCAGATGCTGTTAAGGGTGGCCGATATCAGAATGACTTCAGTACTTGGAAGAACTCTGAAACTCCACCACTGTCTTCATGGAAACCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

343

Amino Acids

37.6

Weight (kDa)

6.52

Isoelectric Point (pI)

31.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 310 7.2e-71 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10810 AT1G10810 AT1G60680 AT1G60690 AT1G60710 AT1G60730 AT1G60730 AT1G60750
fragaria_vesca FvH4_4g12490
malus_domestica MD04G1026100.v1.1 MD09G1032900.v1.1 MD13G1279900.v1.1
prunus_persica Prupe.1G175500_v2.0.a1 Prupe.1G175600_v2.0.a1 Prupe.1G175700_v2.0.a1 Prupe.1G175800_v2.0.a1 Prupe.2G050800_v2.0.a1
pyrus_communis pycom04g02210 pycom04g02230 pycom111g02620 pycom13g29370
rosa_chinensis RchiOBHm_Chr4g0409691 RchiOBHm_Chr4g0410671 RchiOBHm_Chr4g0410821 RchiOBHm_Chr4g0411011 RchiOBHm_Chr4g0411051 RchiOBHm_Chr4g0411101 RchiOBHm_Chr4g0411151 RchiOBHm_Chr4g0411171 RchiOBHm_Chr4g0411201
rosa_laevigata RLG00000008369 RLG00000008372 RLG00000008374 RLG00000008376 RLG00000008378 RLG00000008381 RLG00000008383 RLG00000008395
rosa_multiflora Rmu_co8497103.1_g000001 Rmu_co8514481.1_g000001 Rmu_sc0000543.1_g000005 Rmu_sc0000543.1_g000006 Rmu_sc0000543.1_g000024 Rmu_sc0000944.1_g000002 Rmu_sc0000944.1_g000011 Rmu_sc0001244.1_g000006 Rmu_sc0001244.1_g000014 Rmu_sc0001244.1_g000017 Rmu_sc0001244.1_g000020 Rmu_sc0003150.1_g000001 Rmu_sc0007879.1_g000001 Rmu_sc0007879.1_g000007 Rmu_sc0009499.1_g000008 Rmu_sc0029727.1_g000001
rosa_roxburghii Rroxscaffold_5G00354230 Rroxscaffold_5G00355560 Rroxscaffold_5G00355580 Rroxscaffold_5G00355600 Rroxscaffold_5G00355650 Rroxscaffold_5G00355710 Rroxscaffold_5G00355730 Rroxscaffold_5G00355760 Rroxscaffold_5G00355800 Rroxscaffold_5G00355840 Rroxscaffold_5G00355850
rosa_rugosa Rorug04G0096400 Rorug04G0105400 Rorug04G0110600 Rorug04G0110700 Rorug05G0412600 Rorug07G0180500
rosa_samantha Rh4AG157500 Rh4BG162500 Rh4BG163400 Rh4CG175300 Rh4CG176100 Rh4CG177100 Rh4CG177200 Rh4CG177400 Rh4CG177500 Rh4CG178000 Rh4CG178100 Rh4CG178300 Rh4DG159900 Rh4DG160200
rosa_wichuraiana Rw4G013600 Rw4G013610 Rw4G013660 Rw4G013690 Rw4G013700 Rw4G013710 Rw4G013720 Rw4G013730 Rw4G013740 Rw4G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AccBSI CCGCTC 2 cut(s) 222, 745
AccI GTMKAC 1 cut(s) 141
AciI CCGC 3 cut(s) 5, 222, 745
AclWI GGATC 3 cut(s) 26, 272, 392
AcoI YGGCCR 2 cut(s) 249, 955
AcsI RAATTY 2 cut(s) 257, 859
AcuI CTGAAG 1 cut(s) 958
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 3 cut(s) 458, 528, 979
AfiI CCNNNNNNNGG 5 cut(s) 211, 261, 492, 838, 839
AgsI TTSAA 5 cut(s) 340, 407, 440, 857, 923
AhlI ACTAGT 1 cut(s) 433
AjnI CCWGG 2 cut(s) 39, 698
AjuI GAANNNNNNNTTGG 2 cut(s) 665, 697
Alw21I GWGCWC 4 cut(s) 426, 726, 779, 883
Alw26I GTCTC 4 cut(s) 52, 294, 401, 470
Alw44I GTGCAC 1 cut(s) 775
AlwI GGATC 3 cut(s) 26, 272, 392
AlwNI CAGNNNCTG 1 cut(s) 944
AoxI GGCC 5 cut(s) 37, 91, 181, 249, 955
ApaLI GTGCAC 1 cut(s) 775
ApeKI GCWGC 2 cut(s) 66, 320
ApoI RAATTY 2 cut(s) 257, 859
Asp700I GAANNNNTTC 1 cut(s) 971
AspS9I GGNCC 3 cut(s) 92, 182, 540
AsuC2I CCSGG 2 cut(s) 305, 840
AsuHPI GGTGA 3 cut(s) 145, 722, 803
AvaII GGWCC 1 cut(s) 540
BaeGI GKGCMC 2 cut(s) 779, 833
BalI TGGCCA 1 cut(s) 251
BanII GRGCYC 3 cut(s) 426, 502, 883
BbsI GAAGAC 1 cut(s) 1002
Bbv12I GWGCWC 4 cut(s) 426, 726, 779, 883
BbvI GCAGC 2 cut(s) 53, 307
BceAI ACGGC 3 cut(s) 106, 122, 196
BciT130I CCWGG 2 cut(s) 41, 700
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 2 cut(s) 305, 840
BcoDI GTCTC 4 cut(s) 52, 294, 401, 470
BcuI ACTAGT 1 cut(s) 433
BfaI CTAG 4 cut(s) 62, 434, 623, 719
BfmI CTRYAG 1 cut(s) 936
BisI GCNGC 2 cut(s) 67, 321
BlpI GCTNAGC 1 cut(s) 915
BlsI GCNGC 2 cut(s) 68, 322
BmcAI AGTACT 1 cut(s) 979
Bme1390I CCNGG 4 cut(s) 41, 305, 700, 840
Bme18I GGWCC 1 cut(s) 540
BmgT120I GGNCC 3 cut(s) 92, 182, 540
BmiI GGNNCC 4 cut(s) 94, 184, 704, 844
BmrFI CCNGG 4 cut(s) 41, 305, 700, 840
BmsI GCATC 3 cut(s) 388, 606, 931
BoxI GACNNNNGTC 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 1002
BpmI CTGGAG 2 cut(s) 62, 554
Bpu1102I GCTNAGC 1 cut(s) 915
BpuEI CTTGAG 2 cut(s) 39, 410
BpuMI CCSGG 2 cut(s) 305, 840
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 3 cut(s) 52, 294, 470
BsaJI CCNNGG 5 cut(s) 205, 346, 698, 706, 814
Bsc4I CCNNNNNNNGG 5 cut(s) 211, 261, 492, 838, 839
BseBI CCWGG 2 cut(s) 41, 700
BseDI CCNNGG 5 cut(s) 205, 346, 698, 706, 814
BseGI GGATG 3 cut(s) 81, 126, 511
BseLI CCNNNNNNNGG 5 cut(s) 211, 261, 492, 838, 839
BseMII CTCAG 3 cut(s) 462, 800, 906
BseRI GAGGAG 3 cut(s) 28, 194, 240
BseSI GKGCMC 2 cut(s) 779, 833
BseXI GCAGC 2 cut(s) 53, 307
BseYI CCCAGC 1 cut(s) 25
BshFI GGCC 5 cut(s) 39, 93, 183, 251, 957
BsiHKAI GWGCWC 4 cut(s) 426, 726, 779, 883
BsiSI CCGG 3 cut(s) 147, 305, 839
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 5 cut(s) 211, 261, 492, 838, 839
BsmAI GTCTC 4 cut(s) 52, 294, 401, 470
BsmBI CGTCTC 1 cut(s) 401
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 5 cut(s) 39, 93, 183, 251, 957
Bso31I GGTCTC 3 cut(s) 52, 294, 470
Bsp1286I GDGCHC 6 cut(s) 426, 502, 726, 779, 833, 883
Bsp1407I TGTACA 1 cut(s) 526
Bsp143I GATC 6 cut(s) 18, 117, 264, 364, 384, 754
Bsp1720I GCTNAGC 1 cut(s) 915
BspACI CCGC 3 cut(s) 5, 222, 745
BspANI GGCC 5 cut(s) 39, 93, 183, 251, 957
BspCNI CTCAG 3 cut(s) 463, 799, 907
BspLI GGNNCC 4 cut(s) 94, 184, 704, 844
BspMAI CTGCAG 1 cut(s) 940
BspPI GGATC 3 cut(s) 26, 272, 392
BspTNI GGTCTC 3 cut(s) 52, 294, 470
BsrBI CCGCTC 2 cut(s) 222, 745
BsrGI TGTACA 1 cut(s) 526
BssECI CCNNGG 5 cut(s) 205, 346, 698, 706, 814
BssMI GATC 6 cut(s) 18, 117, 264, 364, 384, 754
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 3 cut(s) 346, 706, 814
Bst2UI CCWGG 2 cut(s) 41, 700
Bst4CI ACNGT 3 cut(s) 415, 617, 1008
Bst6I CTCTTC 1 cut(s) 622
BstACI GRCGYC 1 cut(s) 150
BstAUI TGTACA 1 cut(s) 526
BstC8I GCNNGC 1 cut(s) 334
BstDEI CTNAG 4 cut(s) 471, 651, 786, 915
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 3 cut(s) 81, 126, 511
BstKTI GATC 6 cut(s) 21, 120, 267, 367, 387, 757
BstMAI GTCTC 4 cut(s) 52, 294, 401, 470
BstMBI GATC 6 cut(s) 18, 117, 264, 364, 384, 754
BstMWI GCNNNNNNNGC 3 cut(s) 31, 329, 342
BstNI CCWGG 2 cut(s) 41, 700
BstPAI GACNNNNGTC 1 cut(s) 393
BstSCI CCNGG 4 cut(s) 39, 303, 698, 838
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 2 cut(s) 779, 833
BstV1I GCAGC 2 cut(s) 53, 307
BstV2I GAAGAC 1 cut(s) 1002
BsuRI GGCC 5 cut(s) 39, 93, 183, 251, 957
BtsCI GGATG 3 cut(s) 81, 126, 511
BtsIMutI CAGTG 1 cut(s) 1004
Cac8I GCNNGC 1 cut(s) 334
CaiI CAGNNNCTG 1 cut(s) 944
Cfr13I GGNCC 3 cut(s) 92, 182, 540
CseI GACGC 1 cut(s) 139
Csp6I GTAC 3 cut(s) 457, 527, 978
CspCI CAANNNNNGTGG 2 cut(s) 835, 870
CviAII CATG 4 cut(s) 70, 115, 503, 1014
CviQI GTAC 3 cut(s) 457, 527, 978
DdeI CTNAG 4 cut(s) 471, 651, 786, 915
DpnI GATC 6 cut(s) 20, 119, 266, 366, 386, 756
DpnII GATC 6 cut(s) 18, 117, 264, 364, 384, 754
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 2 cut(s) 249, 955
Eam1104I CTCTTC 1 cut(s) 622
EarI CTCTTC 1 cut(s) 622
Ecl136II GAGCTC 2 cut(s) 424, 881
Eco130I CCWWGG 3 cut(s) 346, 706, 814
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 3 cut(s) 426, 502, 883
Eco31I GGTCTC 3 cut(s) 52, 294, 470
Eco32I GATATC 1 cut(s) 962
Eco47I GGWCC 1 cut(s) 540
Eco53kI GAGCTC 2 cut(s) 424, 881
Eco57I CTGAAG 1 cut(s) 958
EcoICRI GAGCTC 2 cut(s) 424, 881
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 2 cut(s) 39, 698
EcoRV GATATC 1 cut(s) 962
EcoT14I CCWWGG 3 cut(s) 346, 706, 814
EcoT38I GRGCYC 3 cut(s) 426, 502, 883
ErhI CCWWGG 3 cut(s) 346, 706, 814
Esp3I CGTCTC 1 cut(s) 401
FaeI CATG 4 cut(s) 73, 118, 506, 1017
FaiI YATR 8 cut(s) 71, 116, 312, 504, 613, 836, 1015, 1027
FaqI GGGAC 1 cut(s) 248
FatI CATG 4 cut(s) 69, 114, 502, 1013
FauI CCCGC 1 cut(s) 738
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 2 cut(s) 67, 321
FokI GGATG 3 cut(s) 88, 113, 498
FriOI GRGCYC 3 cut(s) 426, 502, 883
Fsp4HI GCNGC 2 cut(s) 67, 321
FspBI CTAG 4 cut(s) 62, 434, 623, 719
GluI GCNGC 2 cut(s) 67, 321
GsaI CCCAGC 1 cut(s) 29
GsuI CTGGAG 2 cut(s) 62, 554
HaeIII GGCC 5 cut(s) 39, 93, 183, 251, 957
HapII CCGG 3 cut(s) 147, 305, 839
HgaI GACGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 4 cut(s) 73, 118, 506, 1017
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 5 cut(s) 143, 199, 351, 633, 923
HpaII CCGG 3 cut(s) 147, 305, 839
HphI GGTGA 3 cut(s) 145, 722, 803
Hpy166II GTNNAC 3 cut(s) 142, 590, 777
Hpy188I TCNGA 6 cut(s) 172, 384, 472, 686, 966, 994
Hpy188III TCNNGA 5 cut(s) 262, 427, 552, 587, 719
Hpy8I GTNNAC 3 cut(s) 142, 590, 777
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 4 cut(s) 94, 166, 437, 763
HpyCH4III ACNGT 3 cut(s) 415, 617, 1008
HpyCH4V TGCA 6 cut(s) 69, 275, 611, 761, 777, 938
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 329, 342
HpyF3I CTNAG 4 cut(s) 471, 651, 786, 915
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 4 cut(s) 73, 118, 506, 1017
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 6 cut(s) 18, 117, 264, 364, 384, 754
LmnI GCTCC 2 cut(s) 421, 878
Lsp1109I GCAGC 2 cut(s) 53, 307
LweI GCATC 3 cut(s) 388, 606, 931
MaeI CTAG 4 cut(s) 62, 434, 623, 719
MaeIII GTNAC 2 cut(s) 151, 409
MalI GATC 6 cut(s) 20, 119, 266, 366, 386, 756
MbiI CCGCTC 2 cut(s) 222, 745
MboI GATC 6 cut(s) 18, 117, 264, 364, 384, 754
MboII GAAGA 5 cut(s) 452, 578, 639, 997, 1002
MfeI CAATTG 1 cut(s) 355
MhlI GDGCHC 6 cut(s) 426, 502, 726, 779, 833, 883
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 4 cut(s) 245, 257, 355, 859
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 2 cut(s) 137, 345
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 251
MroXI GAANNNNTTC 1 cut(s) 971
MscI TGGCCA 1 cut(s) 251
MseI TTAA 2 cut(s) 864, 948
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 2 cut(s) 524, 532
MspI CCGG 3 cut(s) 147, 305, 839
MspR9I CCNGG 4 cut(s) 41, 305, 700, 840
MunI CAATTG 1 cut(s) 355
MvaI CCWGG 2 cut(s) 41, 700
MwoI GCNNNNNNNGC 3 cut(s) 31, 329, 342
NciI CCSGG 2 cut(s) 305, 840
NdeII GATC 6 cut(s) 18, 117, 264, 364, 384, 754
NlaIII CATG 4 cut(s) 73, 118, 506, 1017
NlaIV GGNNCC 4 cut(s) 94, 184, 704, 844
NmeAIII GCCGAG 2 cut(s) 29, 186
NmuCI GTSAC 2 cut(s) 151, 409
PceI AGGCCT 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PdmI GAANNNNTTC 1 cut(s) 971
PfeI GAWTC 3 cut(s) 199, 633, 923
PkrI GCNGC 2 cut(s) 68, 322
PleI GAGTC 2 cut(s) 137, 345
PpsI GAGTC 2 cut(s) 137, 345
PshAI GACNNNNGTC 1 cut(s) 393
Psp124BI GAGCTC 2 cut(s) 426, 883
Psp6I CCWGG 2 cut(s) 39, 698
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 2 cut(s) 39, 698
PspN4I GGNNCC 4 cut(s) 94, 184, 704, 844
PspPI GGNCC 3 cut(s) 92, 182, 540
PstI CTGCAG 1 cut(s) 940
PstNI CAGNNNCTG 1 cut(s) 944
PvuII CAGCTG 2 cut(s) 524, 532
RsaI GTAC 3 cut(s) 458, 528, 979
RsaNI GTAC 3 cut(s) 457, 527, 978
RseI CAYNNNNRTG 1 cut(s) 74
SacI GAGCTC 2 cut(s) 426, 883
SalI GTCGAC 1 cut(s) 140
SaqAI TTAA 2 cut(s) 864, 948
SatI GCNGC 2 cut(s) 67, 321
Sau3AI GATC 6 cut(s) 18, 117, 264, 364, 384, 754
Sau96I GGNCC 3 cut(s) 92, 182, 540
ScaI AGTACT 1 cut(s) 979
SchI GAGTC 2 cut(s) 137, 345
ScrFI CCNGG 4 cut(s) 41, 305, 700, 840
SduI GDGCHC 6 cut(s) 426, 502, 726, 779, 833, 883
SfaNI GCATC 3 cut(s) 388, 606, 931
SfcI CTRYAG 1 cut(s) 936
SinI GGWCC 1 cut(s) 540
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 2 cut(s) 54, 425
SmoI CTYRAG 2 cut(s) 54, 425
SpeI ACTAGT 1 cut(s) 433
Sse9I AATT 4 cut(s) 245, 257, 355, 859
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 3 cut(s) 5, 222, 745
SspI AATATT 2 cut(s) 738, 874
SspMI CTAG 4 cut(s) 62, 434, 623, 719
SstI GAGCTC 2 cut(s) 426, 883
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 4 cut(s) 39, 303, 698, 838
StyI CCWWGG 3 cut(s) 346, 706, 814
TaaI ACNGT 3 cut(s) 415, 617, 1008
TaqI TCGA 4 cut(s) 141, 162, 387, 741
TasI AATT 4 cut(s) 245, 257, 355, 859
TatI WGTACW 3 cut(s) 456, 526, 977
TfiI GAWTC 3 cut(s) 199, 633, 923
Tru1I TTAA 2 cut(s) 864, 948
Tru9I TTAA 2 cut(s) 864, 948
TscAI CASTG 1 cut(s) 1011
TseFI GTSAC 2 cut(s) 151, 409
TseI GCWGC 2 cut(s) 66, 320
Tsp45I GTSAC 2 cut(s) 151, 409
TspDTI ATGAA 3 cut(s) 500, 797, 1002
TspRI CASTG 1 cut(s) 1011
VneI GTGCAC 1 cut(s) 775
VpaK11BI GGWCC 1 cut(s) 540
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 2 cut(s) 257, 859
XbaI TCTAGA 1 cut(s) 718
XcmI CCANNNNNNNNNTGG 1 cut(s) 1011
XmiI GTMKAC 1 cut(s) 141
XmnI GAANNNNTTC 1 cut(s) 971
XspI CTAG 4 cut(s) 62, 434, 623, 719
ZrmI AGTACT 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.