MD09G1032900.v1.1

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000435
Physical Location & Seq
Forward (+)
1999273 .. 2001807
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1032900.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGAAGTTGGGTTCACAAGGCCTCGAGGTGTCGGCTCAAGGCCTTGGCTGCATGGGCATGTCCGCCTTCTACGGCGCTCCAAAGCCCGACCAAGACATGATCAGTCTCATCCACCATGCCATTAACAGCGGGGTCACCTTCCTTGATACCTCTGACATCTACGGTCCCTTCACCAACGAAATCCTTCTTGGCAAGGCTCTCAAGGGAGGGGTACGAGACAAGGTTGAATTGGCCACCAAGTTTGGTCTCAGATTTGTGGAGAACAAGATGGAGGTGGGGAAAAACATGGAGGTGCAAGGTGACCCTGCTTATGTGAGAGCTGCTCTCGAGAGTAGCTTGAAGCGCCTTGGTGTTGATTCTATTGATCTCTATTATCAGCATCGCATTGACACCTCCGTCCCCGTTGAAGTCACGGTTGGGGAACTGAAGAAATTAGTTGAAGAGGGTAAGGTAAAGTACATTGGTCTATCTGAGGCTTCAGCTTCAACGATAAGAAGAGCCCATGCTGTTCATCCTATAACAGCTGTGCAGTTGGAGTGGTCCTTGTGGTCAAGAGATGTCGAGCAAGAAATAATTCCTACTTGTCGGGAGCTTGGAATTGGCATTGTTGCGTATAGTCCTTTAGGAAGAGGATTCTTCGCATCAGGTGCTAAGTTTGTCGAAAACCTTGCCCACGGTGATTCCCGAAAGAATCTACCGAGGTTCCAACCCGAAAATGTAGAGCACAACAAAACACTATTCGAGCGAGTTAGTGATCTTGCTGCAAGGAAAGGGTGCACACCATCTCAGCTAGCATTGGCCTGGGTTCATCACCAAGGAAATGATGTGTGTCCTATCCCCGGAACCACCAAGATTGCGAACTTTGACCAGAATATCGCAGCTCTGTCTGTTAAACTGACACCGGAAGAGTTGGCTGAGCTTGAATCTTATGCTTCAGTAGATGCCGTTAAAGGTGAAAGATATCCGAACTATTATAGCACTTGGTCGAACTCCGAAACTCCGCCACTGTCTTCGTCAAACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

342

Amino Acids

37.36

Weight (kDa)

5.89

Isoelectric Point (pI)

31.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 17 - 309 6.8e-69 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10810 AT1G10810 AT1G60680 AT1G60690 AT1G60710 AT1G60730 AT1G60730 AT1G60750
fragaria_vesca FvH4_4g12490
malus_domestica MD04G1026100.v1.1 MD09G1032900.v1.1 MD13G1279900.v1.1
prunus_persica Prupe.1G175500_v2.0.a1 Prupe.1G175600_v2.0.a1 Prupe.1G175700_v2.0.a1 Prupe.1G175800_v2.0.a1 Prupe.2G050800_v2.0.a1
pyrus_communis pycom04g02210 pycom04g02230 pycom111g02620 pycom13g29370
rosa_chinensis RchiOBHm_Chr4g0409691 RchiOBHm_Chr4g0410671 RchiOBHm_Chr4g0410821 RchiOBHm_Chr4g0411011 RchiOBHm_Chr4g0411051 RchiOBHm_Chr4g0411101 RchiOBHm_Chr4g0411151 RchiOBHm_Chr4g0411171 RchiOBHm_Chr4g0411201
rosa_laevigata RLG00000008369 RLG00000008372 RLG00000008374 RLG00000008376 RLG00000008378 RLG00000008381 RLG00000008383 RLG00000008395
rosa_multiflora Rmu_co8497103.1_g000001 Rmu_co8514481.1_g000001 Rmu_sc0000543.1_g000005 Rmu_sc0000543.1_g000006 Rmu_sc0000543.1_g000024 Rmu_sc0000944.1_g000002 Rmu_sc0000944.1_g000011 Rmu_sc0001244.1_g000006 Rmu_sc0001244.1_g000014 Rmu_sc0001244.1_g000017 Rmu_sc0001244.1_g000020 Rmu_sc0003150.1_g000001 Rmu_sc0007879.1_g000001 Rmu_sc0007879.1_g000007 Rmu_sc0009499.1_g000008 Rmu_sc0029727.1_g000001
rosa_roxburghii Rroxscaffold_5G00354230 Rroxscaffold_5G00355560 Rroxscaffold_5G00355580 Rroxscaffold_5G00355600 Rroxscaffold_5G00355650 Rroxscaffold_5G00355710 Rroxscaffold_5G00355730 Rroxscaffold_5G00355760 Rroxscaffold_5G00355800 Rroxscaffold_5G00355840 Rroxscaffold_5G00355850
rosa_rugosa Rorug04G0096400 Rorug04G0105400 Rorug04G0110600 Rorug04G0110700 Rorug05G0412600 Rorug07G0180500
rosa_samantha Rh4AG157500 Rh4BG162500 Rh4BG163400 Rh4CG175300 Rh4CG176100 Rh4CG177100 Rh4CG177200 Rh4CG177400 Rh4CG177500 Rh4CG178000 Rh4CG178100 Rh4CG178300 Rh4DG159900 Rh4DG160200
rosa_wichuraiana Rw4G013600 Rw4G013610 Rw4G013660 Rw4G013690 Rw4G013700 Rw4G013710 Rw4G013720 Rw4G013730 Rw4G013740 Rw4G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AbsI CCTCGAGG 1 cut(s) 23
AciI CCGC 3 cut(s) 63, 129, 1001
AcoI YGGCCR 1 cut(s) 231
AcuI CTGAAG 3 cut(s) 446, 462, 918
AfaI GTAC 2 cut(s) 213, 458
AfiI CCNNNNNNNGG 1 cut(s) 839
AgsI TTSAA 6 cut(s) 227, 340, 407, 440, 486, 923
AjnI CCWGG 1 cut(s) 800
AjuI GAANNNNNNNTTGG 4 cut(s) 171, 203, 399, 431
AluBI AGCT 8 cut(s) 320, 336, 482, 524, 592, 790, 881, 919
AluI AGCT 8 cut(s) 320, 336, 482, 524, 592, 790, 881, 919
Alw21I GWGCWC 2 cut(s) 726, 779
Alw26I GTCTC 3 cut(s) 110, 210, 251
Alw44I GTGCAC 1 cut(s) 775
Ama87I CYCGRG 2 cut(s) 23, 326
AoxI GGCC 4 cut(s) 19, 40, 231, 798
ApaLI GTGCAC 1 cut(s) 775
ApeKI GCWGC 4 cut(s) 48, 320, 761, 878
Asp700I GAANNNNTTC 2 cut(s) 183, 573
AspLEI GCGC 2 cut(s) 77, 345
AspS9I GGNCC 2 cut(s) 164, 540
AsuC2I CCSGG 1 cut(s) 840
AsuHPI GGTGA 6 cut(s) 127, 163, 311, 689, 803, 965
AsuNHI GCTAGC 1 cut(s) 790
AvaI CYCGRG 2 cut(s) 23, 326
AvaII GGWCC 2 cut(s) 164, 540
BaeGI GKGCMC 1 cut(s) 779
BalI TGGCCA 1 cut(s) 233
BanII GRGCYC 1 cut(s) 502
BbsI GAAGAC 1 cut(s) 1002
Bbv12I GWGCWC 2 cut(s) 726, 779
BbvI GCAGC 4 cut(s) 35, 307, 748, 890
BccI CCATC 2 cut(s) 262, 790
BceAI ACGGC 2 cut(s) 88, 929
BcgI CGANNNNNNTGC 2 cut(s) 650, 684
BciT130I CCWGG 1 cut(s) 802
BclI TGATCA 1 cut(s) 99
BcnI CCSGG 1 cut(s) 840
BcoDI GTCTC 3 cut(s) 110, 210, 251
BfaI CTAG 1 cut(s) 791
BfoI RGCGCY 2 cut(s) 78, 346
BisI GCNGC 4 cut(s) 49, 321, 762, 879
BlpI GCTNAGC 1 cut(s) 915
BlsI GCNGC 4 cut(s) 50, 322, 763, 880
Bme1390I CCNGG 2 cut(s) 802, 840
Bme18I GGWCC 2 cut(s) 164, 540
BmeT110I CYCGRG 2 cut(s) 23, 326
BmgT120I GGNCC 2 cut(s) 164, 540
BmiI GGNNCC 3 cut(s) 166, 704, 844
BmrFI CCNGG 2 cut(s) 802, 840
BmsI GCATC 3 cut(s) 388, 650, 931
BmtI GCTAGC 1 cut(s) 794
BpiI GAAGAC 1 cut(s) 1002
BplI GAGNNNNNCTC 4 cut(s) 307, 339, 309, 341
Bpu1102I GCTNAGC 1 cut(s) 915
BpuEI CTTGAG 2 cut(s) 21, 185
BpuMI CCSGG 1 cut(s) 840
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 7 cut(s) 43, 346, 673, 698, 801, 814, 838
BsaWI WCCGGW 1 cut(s) 901
Bsc4I CCNNNNNNNGG 1 cut(s) 839
BseBI CCWGG 1 cut(s) 802
BseDI CCNNGG 7 cut(s) 43, 346, 673, 698, 801, 814, 838
BseGI GGATG 2 cut(s) 108, 511
BseLI CCNNNNNNNGG 1 cut(s) 839
BseMII CTCAG 4 cut(s) 262, 462, 800, 906
BseSI GKGCMC 1 cut(s) 779
BseXI GCAGC 4 cut(s) 35, 307, 748, 890
BsgI GTGCAG 1 cut(s) 548
BshFI GGCC 4 cut(s) 21, 42, 233, 800
BsiHKAI GWGCWC 2 cut(s) 726, 779
BsiHKCI CYCGRG 2 cut(s) 23, 326
BsiSI CCGG 2 cut(s) 840, 902
BslFI GGGAC 2 cut(s) 150, 383
BslI CCNNNNNNNGG 1 cut(s) 839
BsmAI GTCTC 3 cut(s) 110, 210, 251
BsmFI GGGAC 2 cut(s) 150, 383
BsnI GGCC 4 cut(s) 21, 42, 233, 800
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 2 cut(s) 23, 326
Bsp1286I GDGCHC 3 cut(s) 502, 726, 779
Bsp143I GATC 3 cut(s) 99, 364, 754
Bsp1720I GCTNAGC 1 cut(s) 915
BspACI CCGC 3 cut(s) 63, 129, 1001
BspANI GGCC 4 cut(s) 21, 42, 233, 800
BspCNI CTCAG 4 cut(s) 261, 463, 799, 907
BspLI GGNNCC 3 cut(s) 166, 704, 844
BspOI GCTAGC 1 cut(s) 794
BspQI GCTCTTC 1 cut(s) 490
BspTNI GGTCTC 1 cut(s) 251
BssECI CCNNGG 7 cut(s) 43, 346, 673, 698, 801, 814, 838
BssMI GATC 3 cut(s) 99, 364, 754
BssT1I CCWWGG 3 cut(s) 43, 346, 814
Bst2UI CCWGG 1 cut(s) 802
Bst4CI ACNGT 4 cut(s) 164, 415, 677, 1008
Bst6I CTCTTC 4 cut(s) 435, 490, 622, 900
BstAPI GCANNNNNTGC 1 cut(s) 647
BstC8I GCNNGC 1 cut(s) 792
BstDEI CTNAG 5 cut(s) 248, 471, 651, 786, 915
BstDSI CCRYGG 1 cut(s) 673
BstEII GGTNACC 2 cut(s) 133, 299
BstF5I GGATG 2 cut(s) 108, 511
BstH2I RGCGCY 2 cut(s) 78, 346
BstHHI GCGC 2 cut(s) 77, 345
BstKTI GATC 3 cut(s) 102, 367, 757
BstMAI GTCTC 3 cut(s) 110, 210, 251
BstMBI GATC 3 cut(s) 99, 364, 754
BstMWI GCNNNNNNNGC 4 cut(s) 48, 54, 342, 647
BstNI CCWGG 1 cut(s) 802
BstNSI RCATGY 1 cut(s) 61
BstPI GGTNACC 2 cut(s) 133, 299
BstSCI CCNGG 2 cut(s) 800, 838
BstSLI GKGCMC 1 cut(s) 779
BstV1I GCAGC 4 cut(s) 35, 307, 748, 890
BstV2I GAAGAC 1 cut(s) 1002
BsuRI GGCC 4 cut(s) 21, 42, 233, 800
BtgI CCRYGG 1 cut(s) 673
BtgZI GCGATG 1 cut(s) 365
BtsCI GGATG 2 cut(s) 108, 511
BtsIMutI CAGTG 1 cut(s) 1004
Cac8I GCNNGC 1 cut(s) 792
CfoI GCGC 2 cut(s) 77, 345
Cfr13I GGNCC 2 cut(s) 164, 540
Csp6I GTAC 2 cut(s) 212, 457
CspCI CAANNNNNGTGG 4 cut(s) 223, 258, 835, 870
CviAII CATG 6 cut(s) 52, 58, 97, 116, 286, 503
CviQI GTAC 2 cut(s) 212, 457
DdeI CTNAG 5 cut(s) 248, 471, 651, 786, 915
DpnI GATC 3 cut(s) 101, 366, 756
DpnII GATC 3 cut(s) 99, 364, 754
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 4 cut(s) 435, 490, 622, 900
EarI CTCTTC 4 cut(s) 435, 490, 622, 900
EciI GGCGGA 2 cut(s) 52, 990
Eco130I CCWWGG 3 cut(s) 43, 346, 814
Eco147I AGGCCT 2 cut(s) 21, 42
Eco24I GRGCYC 1 cut(s) 502
Eco31I GGTCTC 1 cut(s) 251
Eco32I GATATC 1 cut(s) 962
Eco47I GGWCC 2 cut(s) 164, 540
Eco57I CTGAAG 3 cut(s) 446, 462, 918
Eco88I CYCGRG 2 cut(s) 23, 326
Eco91I GGTNACC 2 cut(s) 133, 299
EcoO65I GGTNACC 2 cut(s) 133, 299
EcoRII CCWGG 1 cut(s) 800
EcoRV GATATC 1 cut(s) 962
EcoT14I CCWWGG 3 cut(s) 43, 346, 814
EcoT38I GRGCYC 1 cut(s) 502
ErhI CCWWGG 3 cut(s) 43, 346, 814
FaeI CATG 6 cut(s) 55, 61, 100, 119, 289, 506
FaqI GGGAC 2 cut(s) 150, 383
FatI CATG 6 cut(s) 51, 57, 96, 115, 285, 502
FauI CCCGC 1 cut(s) 122
FbaI TGATCA 1 cut(s) 99
Fnu4HI GCNGC 4 cut(s) 49, 321, 762, 879
FokI GGATG 2 cut(s) 95, 498
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 4 cut(s) 49, 321, 762, 879
FspBI CTAG 1 cut(s) 791
GlaI GCGC 2 cut(s) 76, 344
GluI GCNGC 4 cut(s) 49, 321, 762, 879
HaeII RGCGCY 2 cut(s) 78, 346
HaeIII GGCC 4 cut(s) 21, 42, 233, 800
HapII CCGG 2 cut(s) 840, 902
HhaI GCGC 2 cut(s) 77, 345
Hin1II CATG 6 cut(s) 55, 61, 100, 119, 289, 506
Hin6I GCGC 2 cut(s) 75, 343
HinP1I GCGC 2 cut(s) 75, 343
HinfI GANTC 5 cut(s) 356, 633, 680, 691, 923
HpaII CCGG 2 cut(s) 840, 902
HphI GGTGA 6 cut(s) 127, 163, 311, 689, 803, 965
Hpy166II GTNNAC 2 cut(s) 14, 777
Hpy188I TCNGA 5 cut(s) 154, 251, 472, 966, 994
Hpy188III TCNNGA 5 cut(s) 326, 328, 552, 587, 684
Hpy8I GTNNAC 2 cut(s) 14, 777
HpyAV CCTTC 4 cut(s) 76, 148, 178, 194
HpyCH4III ACNGT 4 cut(s) 164, 415, 677, 1008
HpyCH4V TGCA 5 cut(s) 51, 295, 529, 764, 777
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 54, 342, 647
HpyF3I CTNAG 5 cut(s) 248, 471, 651, 786, 915
Hsp92II CATG 6 cut(s) 55, 61, 100, 119, 289, 506
HspAI GCGC 2 cut(s) 75, 343
Ksp22I TGATCA 1 cut(s) 99
Kzo9I GATC 3 cut(s) 99, 364, 754
LguI GCTCTTC 1 cut(s) 490
LmnI GCTCC 2 cut(s) 82, 589
LpnPI CCDG 7 cut(s) 318, 630, 787, 814, 853, 881, 915
Lsp1109I GCAGC 4 cut(s) 35, 307, 748, 890
LweI GCATC 3 cut(s) 388, 650, 931
MaeI CTAG 1 cut(s) 791
MaeIII GTNAC 3 cut(s) 133, 299, 409
MalI GATC 3 cut(s) 101, 366, 756
MboI GATC 3 cut(s) 99, 364, 754
MboII GAAGA 7 cut(s) 439, 452, 507, 628, 639, 917, 1002
MhlI GDGCHC 3 cut(s) 502, 726, 779
MlsI TGGCCA 1 cut(s) 233
MluCI AATT 4 cut(s) 227, 431, 573, 597
MluNI TGGCCA 1 cut(s) 233
MmeI TCCRAC 2 cut(s) 513, 730
Mox20I TGGCCA 1 cut(s) 233
MroXI GAANNNNTTC 2 cut(s) 183, 573
MscI TGGCCA 1 cut(s) 233
MseI TTAA 3 cut(s) 123, 891, 948
MslI CAYNNNNRTG 2 cut(s) 56, 290
Msp20I TGGCCA 1 cut(s) 233
MspA1I CMGCKG 2 cut(s) 129, 524
MspI CCGG 2 cut(s) 840, 902
MspR9I CCNGG 2 cut(s) 802, 840
MvaI CCWGG 1 cut(s) 802
MwoI GCNNNNNNNGC 4 cut(s) 48, 54, 342, 647
NciI CCSGG 1 cut(s) 840
NdeII GATC 3 cut(s) 99, 364, 754
NheI GCTAGC 1 cut(s) 790
NlaIII CATG 6 cut(s) 55, 61, 100, 119, 289, 506
NlaIV GGNNCC 3 cut(s) 166, 704, 844
NmuCI GTSAC 3 cut(s) 133, 299, 409
NspI RCATGY 1 cut(s) 61
PaeR7I CTCGAG 2 cut(s) 23, 326
PceI AGGCCT 2 cut(s) 21, 42
PciSI GCTCTTC 1 cut(s) 490
PdmI GAANNNNTTC 2 cut(s) 183, 573
PfeI GAWTC 5 cut(s) 356, 633, 680, 691, 923
PkrI GCNGC 4 cut(s) 50, 322, 763, 880
Psp6I CCWGG 1 cut(s) 800
PspEI GGTNACC 2 cut(s) 133, 299
PspGI CCWGG 1 cut(s) 800
PspN4I GGNNCC 3 cut(s) 166, 704, 844
PspPI GGNCC 2 cut(s) 164, 540
PspXI VCTCGAGB 1 cut(s) 23
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 2 cut(s) 213, 458
RsaNI GTAC 2 cut(s) 212, 457
RseI CAYNNNNRTG 2 cut(s) 56, 290
SapI GCTCTTC 1 cut(s) 490
SaqAI TTAA 3 cut(s) 123, 891, 948
SatI GCNGC 4 cut(s) 49, 321, 762, 879
Sau3AI GATC 3 cut(s) 99, 364, 754
Sau96I GGNCC 2 cut(s) 164, 540
ScrFI CCNGG 2 cut(s) 802, 840
SduI GDGCHC 3 cut(s) 502, 726, 779
SfaNI GCATC 3 cut(s) 388, 650, 931
Sfr274I CTCGAG 2 cut(s) 23, 326
SinI GGWCC 2 cut(s) 164, 540
SlaI CTCGAG 2 cut(s) 23, 326
SmiMI CAYNNNNRTG 2 cut(s) 56, 290
SmlI CTYRAG 4 cut(s) 23, 36, 200, 326
SmoI CTYRAG 4 cut(s) 23, 36, 200, 326
Sse9I AATT 4 cut(s) 227, 431, 573, 597
SseBI AGGCCT 2 cut(s) 21, 42
SsiI CCGC 3 cut(s) 63, 129, 1001
SspMI CTAG 1 cut(s) 791
StuI AGGCCT 2 cut(s) 21, 42
StyD4I CCNGG 2 cut(s) 800, 838
StyI CCWWGG 3 cut(s) 43, 346, 814
TaaI ACNGT 4 cut(s) 164, 415, 677, 1008
TaqI TCGA 6 cut(s) 24, 327, 561, 660, 741, 986
TasI AATT 4 cut(s) 227, 431, 573, 597
TatI WGTACW 1 cut(s) 456
TfiI GAWTC 5 cut(s) 356, 633, 680, 691, 923
Tru1I TTAA 3 cut(s) 123, 891, 948
Tru9I TTAA 3 cut(s) 123, 891, 948
TscAI CASTG 1 cut(s) 1011
TseFI GTSAC 3 cut(s) 133, 299, 409
TseI GCWGC 4 cut(s) 48, 320, 761, 878
Tsp45I GTSAC 3 cut(s) 133, 299, 409
TspDTI ATGAA 3 cut(s) 17, 500, 797
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 1 cut(s) 1011
VneI GTGCAC 1 cut(s) 775
VpaK11BI GGWCC 2 cut(s) 164, 540
XceI RCATGY 1 cut(s) 61
XhoI CTCGAG 2 cut(s) 23, 326
XmnI GAANNNNTTC 2 cut(s) 183, 573
XspI CTAG 1 cut(s) 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.