Rw4G013740

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000435
Physical Location & Seq
Forward (+)
32144827 .. 32148599
3773 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013740.1

Sequence Viewer

Length: 1029 bp
ATGGCGGGTGTGAGGAGGATGAAGCTCGGGTCACAAGGCCTGGAGGTGTCAGCTCAAGGACTGGGCTGCATGGGCATGTCAGCCTTCTATGGCCCCCCGAAGCCCGAAGCCGACATGATCAAGCTCATCCACCATGCCGTCGACTCCGGCGTCACCTTCCTCGACACCTCCGACATGTACGGTCCCCACACCAACGAAATGCTCCTCGGCAAGGCTTTGAGTGGAGGGCTGAGAGACAAGGTTGAATTGACCACCAAATTCGGTGCCAGGTTTGCGGACAACAAAATGGAGATTCGAGGCGAGCCGGCATATGTGAGAGCTTCTTGTGAAGGAAGCTTGAAACGACTTGGTGTAGATTCTATTGATCTCTATTATCAGCATAGGATTGACACCCGTGTCCCCATTGAAGTCACGGTGGGGGAGCTCAAGAAGCTAGTTGAAGAGGGTAAAATAAAGTATATTGGTTTGTCTGAGGCATCAGCTTCAGCAATAAGAAGAGCCCATTGTGTTCATCCCATAACTGCTGTGCAGCTGGAGTGGTCCTTGTGGTCAAGAGATGTAGAGGAAGAAATAATCCCTACTTGTCGGGAACTTGGCATTGGCATTGTTGCATACAGTCCTCTAGGAAGAGGATTCTTATCATCGGGGGCAAAATTCGTCGAAAATCTTCCCAATGATGATGTTCGAAAGTATATGCCCAGGTTCCAAGCTGAAAACCTAGAGCATAATAAAACAATATTTGAGCGAGTAAGTGATCTTGCAGCAAGGAAGGGTTGCATCCCATCTCAACTAGCACTGGCCTGGGTTCATCACCAAGGAAATGATGTGTGCCCTATACCCGGAACCACCAAGATTGAGAATTTTAACCAGAACATTAAGGCTCTTTCTGTGAAACTGACACCAGAAGAAATGGCTGAGCTTGAATCTTTTGCTTCCGCAGATGCTGTTAAGGGCGACAGGTATCAGACTAGCGTTAGTACTTGGAGGAACTCTGAGACTCCACCACTCTCTTCATGGAAGGCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

342

Amino Acids

37.74

Weight (kDa)

6.52

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 310 7.6e-70 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10810 AT1G10810 AT1G60680 AT1G60690 AT1G60710 AT1G60730 AT1G60730 AT1G60750
fragaria_vesca FvH4_4g12490
malus_domestica MD04G1026100.v1.1 MD09G1032900.v1.1 MD13G1279900.v1.1
prunus_persica Prupe.1G175500_v2.0.a1 Prupe.1G175600_v2.0.a1 Prupe.1G175700_v2.0.a1 Prupe.1G175800_v2.0.a1 Prupe.2G050800_v2.0.a1
pyrus_communis pycom04g02210 pycom04g02230 pycom111g02620 pycom13g29370
rosa_chinensis RchiOBHm_Chr4g0409691 RchiOBHm_Chr4g0410671 RchiOBHm_Chr4g0410821 RchiOBHm_Chr4g0411011 RchiOBHm_Chr4g0411051 RchiOBHm_Chr4g0411101 RchiOBHm_Chr4g0411151 RchiOBHm_Chr4g0411171 RchiOBHm_Chr4g0411201
rosa_laevigata RLG00000008369 RLG00000008372 RLG00000008374 RLG00000008376 RLG00000008378 RLG00000008381 RLG00000008383 RLG00000008395
rosa_multiflora Rmu_co8497103.1_g000001 Rmu_co8514481.1_g000001 Rmu_sc0000543.1_g000005 Rmu_sc0000543.1_g000006 Rmu_sc0000543.1_g000024 Rmu_sc0000944.1_g000002 Rmu_sc0000944.1_g000011 Rmu_sc0001244.1_g000006 Rmu_sc0001244.1_g000014 Rmu_sc0001244.1_g000017 Rmu_sc0001244.1_g000020 Rmu_sc0003150.1_g000001 Rmu_sc0007879.1_g000001 Rmu_sc0007879.1_g000007 Rmu_sc0009499.1_g000008 Rmu_sc0029727.1_g000001
rosa_roxburghii Rroxscaffold_5G00354230 Rroxscaffold_5G00355560 Rroxscaffold_5G00355580 Rroxscaffold_5G00355600 Rroxscaffold_5G00355650 Rroxscaffold_5G00355710 Rroxscaffold_5G00355730 Rroxscaffold_5G00355760 Rroxscaffold_5G00355800 Rroxscaffold_5G00355840 Rroxscaffold_5G00355850
rosa_rugosa Rorug04G0096400 Rorug04G0105400 Rorug04G0110600 Rorug04G0110700 Rorug05G0412600 Rorug07G0180500
rosa_samantha Rh4AG157500 Rh4BG162500 Rh4BG163400 Rh4CG175300 Rh4CG176100 Rh4CG177100 Rh4CG177200 Rh4CG177400 Rh4CG177500 Rh4CG178000 Rh4CG178100 Rh4CG178300 Rh4DG159900 Rh4DG160200
rosa_wichuraiana Rw4G013600 Rw4G013610 Rw4G013660 Rw4G013690 Rw4G013700 Rw4G013710 Rw4G013720 Rw4G013730 Rw4G013740 Rw4G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 149, 395
AccB1I GGYRCC 1 cut(s) 263
AccI GTMKAC 1 cut(s) 141
AciI CCGC 3 cut(s) 5, 275, 936
AcsI RAATTY 3 cut(s) 257, 653, 859
AcuI CTGAAG 1 cut(s) 468
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 2 cut(s) 179, 979
AfiI CCNNNNNNNGG 2 cut(s) 211, 839
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 5 cut(s) 245, 340, 407, 440, 923
AjnI CCWGG 4 cut(s) 39, 266, 698, 800
AjuI GAANNNNNNNTTGG 2 cut(s) 582, 614
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 2 cut(s) 228, 989
AlwNI CAGNNNCTG 1 cut(s) 944
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 4 cut(s) 37, 91, 798, 1020
ApeKI GCWGC 3 cut(s) 66, 529, 761
ApoI RAATTY 3 cut(s) 257, 653, 859
Asp700I GAANNNNTTC 1 cut(s) 666
AspS9I GGNCC 3 cut(s) 92, 182, 540
AsuC2I CCSGG 1 cut(s) 840
AsuHPI GGTGA 2 cut(s) 145, 803
AsuII TTCGAA 1 cut(s) 685
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 2 cut(s) 182, 540
BaeGI GKGCMC 1 cut(s) 833
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 2 cut(s) 426, 502
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 3 cut(s) 53, 541, 773
BccI CCATC 1 cut(s) 790
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 4 cut(s) 41, 268, 700, 802
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 1 cut(s) 840
BcoDI GTCTC 2 cut(s) 228, 989
BfaI CTAG 5 cut(s) 434, 623, 719, 791, 969
BisI GCNGC 3 cut(s) 67, 530, 762
BlpI GCTNAGC 1 cut(s) 915
BlsI GCNGC 3 cut(s) 68, 531, 763
BmcAI AGTACT 1 cut(s) 979
Bme1390I CCNGG 5 cut(s) 41, 268, 700, 802, 840
Bme18I GGWCC 2 cut(s) 182, 540
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 3 cut(s) 92, 182, 540
BmiI GGNNCC 5 cut(s) 94, 184, 265, 704, 844
BmrFI CCNGG 5 cut(s) 41, 268, 700, 802, 840
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 3 cut(s) 485, 786, 931
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 2 cut(s) 62, 554
Bpu1102I GCTNAGC 1 cut(s) 915
Bpu14I TTCGAA 1 cut(s) 685
BpuEI CTTGAG 2 cut(s) 39, 410
BpuMI CCSGG 1 cut(s) 840
BsaBI GATNNNNATC 1 cut(s) 637
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 4 cut(s) 205, 698, 801, 814
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 839
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 2 cut(s) 66, 801
Bse8I GATNNNNATC 1 cut(s) 637
BseBI CCWGG 4 cut(s) 41, 268, 700, 802
BseDI CCNNGG 4 cut(s) 205, 698, 801, 814
BseGI GGATG 4 cut(s) 24, 126, 511, 777
BseJI GATNNNNATC 1 cut(s) 637
BseLI CCNNNNNNNGG 2 cut(s) 211, 839
BseMII CTCAG 4 cut(s) 221, 462, 906, 984
BseNI ACTGG 2 cut(s) 66, 801
BseRI GAGGAG 2 cut(s) 28, 194
BseSI GKGCMC 1 cut(s) 833
BseXI GCAGC 3 cut(s) 53, 541, 773
BsgI GTGCAG 1 cut(s) 548
BshFI GGCC 4 cut(s) 39, 93, 800, 1022
BshNI GGYRCC 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 3 cut(s) 147, 305, 840
BslFI GGGAC 2 cut(s) 168, 383
BslI CCNNNNNNNGG 2 cut(s) 211, 839
BsmAI GTCTC 2 cut(s) 228, 989
BsmFI GGGAC 2 cut(s) 168, 383
BsnI GGCC 4 cut(s) 39, 93, 800, 1022
BsoBI CYCGRG 1 cut(s) 26
Bsp119I TTCGAA 1 cut(s) 685
Bsp1286I GDGCHC 3 cut(s) 426, 502, 833
Bsp143I GATC 3 cut(s) 117, 364, 754
Bsp1720I GCTNAGC 1 cut(s) 915
BspACI CCGC 3 cut(s) 5, 275, 936
BspANI GGCC 4 cut(s) 39, 93, 800, 1022
BspCNI CTCAG 4 cut(s) 222, 463, 907, 985
BspLI GGNNCC 5 cut(s) 94, 184, 265, 704, 844
BspQI GCTCTTC 1 cut(s) 490
BspT104I TTCGAA 1 cut(s) 685
BspT107I GGYRCC 1 cut(s) 263
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 2 cut(s) 66, 801
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 4 cut(s) 205, 698, 801, 814
BssMI GATC 3 cut(s) 117, 364, 754
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 814
Bst2UI CCWGG 4 cut(s) 41, 268, 700, 802
Bst4CI ACNGT 3 cut(s) 182, 415, 617
Bst6I CTCTTC 4 cut(s) 435, 490, 622, 1015
BstACI GRCGYC 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 685
BstC8I GCNNGC 2 cut(s) 302, 306
BstDEI CTNAG 4 cut(s) 230, 471, 915, 993
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 4 cut(s) 24, 126, 511, 777
BstKTI GATC 3 cut(s) 120, 367, 757
BstMAI GTCTC 2 cut(s) 228, 989
BstMBI GATC 3 cut(s) 117, 364, 754
BstMWI GCNNNNNNNGC 3 cut(s) 72, 272, 430
BstNI CCWGG 4 cut(s) 41, 268, 700, 802
BstNSI RCATGY 2 cut(s) 79, 178
BstSCI CCNGG 5 cut(s) 39, 266, 698, 800, 838
BstSLI GKGCMC 1 cut(s) 833
BstV1I GCAGC 3 cut(s) 53, 541, 773
BsuRI GGCC 4 cut(s) 39, 93, 800, 1022
BtsCI GGATG 4 cut(s) 24, 126, 511, 777
BtsIMutI CAGTG 1 cut(s) 794
Cac8I GCNNGC 2 cut(s) 302, 306
CaiI CAGNNNCTG 1 cut(s) 944
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 3 cut(s) 92, 182, 540
CseI GACGC 1 cut(s) 139
Csp6I GTAC 2 cut(s) 178, 978
CspCI CAANNNNNGTGG 2 cut(s) 835, 870
CviAII CATG 6 cut(s) 70, 76, 115, 134, 175, 1014
CviQI GTAC 2 cut(s) 178, 978
DdeI CTNAG 4 cut(s) 230, 471, 915, 993
DpnI GATC 3 cut(s) 119, 366, 756
DpnII GATC 3 cut(s) 117, 364, 754
DrdI GACNNNNNNGTC 2 cut(s) 149, 395
DseDI GACNNNNNNGTC 2 cut(s) 149, 395
Eam1104I CTCTTC 4 cut(s) 435, 490, 622, 1015
EarI CTCTTC 4 cut(s) 435, 490, 622, 1015
Ecl136II GAGCTC 1 cut(s) 424
Eco130I CCWWGG 1 cut(s) 814
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 2 cut(s) 426, 502
Eco47I GGWCC 2 cut(s) 182, 540
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 468
Eco88I CYCGRG 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 424
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 4 cut(s) 39, 266, 698, 800
EcoT14I CCWWGG 1 cut(s) 814
EcoT38I GRGCYC 2 cut(s) 426, 502
ErhI CCWWGG 1 cut(s) 814
FaeI CATG 6 cut(s) 73, 79, 118, 137, 178, 1017
FaqI GGGAC 2 cut(s) 168, 383
FatI CATG 6 cut(s) 69, 75, 114, 133, 174, 1013
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 3 cut(s) 67, 530, 762
FokI GGATG 4 cut(s) 31, 113, 498, 764
FriOI GRGCYC 2 cut(s) 426, 502
Fsp4HI GCNGC 3 cut(s) 67, 530, 762
FspBI CTAG 5 cut(s) 434, 623, 719, 791, 969
GluI GCNGC 3 cut(s) 67, 530, 762
GsuI CTGGAG 2 cut(s) 62, 554
HaeIII GGCC 4 cut(s) 39, 93, 800, 1022
HapII CCGG 3 cut(s) 147, 305, 840
HgaI GACGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 6 cut(s) 73, 79, 118, 137, 178, 1017
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 6 cut(s) 143, 292, 356, 633, 923, 997
HpaII CCGG 3 cut(s) 147, 305, 840
HphI GGTGA 2 cut(s) 145, 803
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 4 cut(s) 172, 472, 966, 994
Hpy188III TCNNGA 3 cut(s) 427, 552, 587
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 2 cut(s) 143, 662
HpyAV CCTTC 5 cut(s) 94, 166, 323, 763, 1012
HpyCH4III ACNGT 3 cut(s) 182, 415, 617
HpyCH4V TGCA 5 cut(s) 69, 529, 611, 761, 777
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 272, 430
HpyF3I CTNAG 4 cut(s) 230, 471, 915, 993
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 6 cut(s) 73, 79, 118, 137, 178, 1017
KroI GCCGGC 1 cut(s) 304
KroNI GCCGGC 1 cut(s) 306
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 117, 364, 754
LguI GCTCTTC 1 cut(s) 490
LmnI GCTCC 2 cut(s) 207, 421
Lsp1109I GCAGC 3 cut(s) 53, 541, 773
LweI GCATC 3 cut(s) 485, 786, 931
MaeI CTAG 5 cut(s) 434, 623, 719, 791, 969
MaeIII GTNAC 3 cut(s) 30, 151, 409
MalI GATC 3 cut(s) 119, 366, 756
MboI GATC 3 cut(s) 117, 364, 754
MboII GAAGA 7 cut(s) 452, 507, 578, 639, 659, 917, 1002
MhlI GDGCHC 3 cut(s) 426, 502, 833
MluCI AATT 4 cut(s) 245, 257, 653, 859
MlyI GAGTC 2 cut(s) 137, 991
MmeI TCCRAC 1 cut(s) 195
MroNI GCCGGC 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 666
MseI TTAA 3 cut(s) 864, 876, 948
MslI CAYNNNNRTG 1 cut(s) 74
MspA1I CMGCKG 1 cut(s) 532
MspI CCGG 3 cut(s) 147, 305, 840
MspR9I CCNGG 5 cut(s) 41, 268, 700, 802, 840
MvaI CCWGG 4 cut(s) 41, 268, 700, 802
MwoI GCNNNNNNNGC 3 cut(s) 72, 272, 430
NaeI GCCGGC 1 cut(s) 306
NciI CCSGG 1 cut(s) 840
NdeI CATATG 1 cut(s) 310
NdeII GATC 3 cut(s) 117, 364, 754
NgoMIV GCCGGC 1 cut(s) 304
NlaIII CATG 6 cut(s) 73, 79, 118, 137, 178, 1017
NlaIV GGNNCC 5 cut(s) 94, 184, 265, 704, 844
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 3 cut(s) 30, 151, 409
NspI RCATGY 2 cut(s) 79, 178
NspV TTCGAA 1 cut(s) 685
PceI AGGCCT 1 cut(s) 39
PciI ACATGT 1 cut(s) 174
PciSI GCTCTTC 1 cut(s) 490
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PdiI GCCGGC 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 666
PfeI GAWTC 4 cut(s) 292, 356, 633, 923
PkrI GCNGC 3 cut(s) 68, 531, 763
PleI GAGTC 2 cut(s) 137, 991
PpsI GAGTC 2 cut(s) 137, 991
PscI ACATGT 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 426
Psp6I CCWGG 4 cut(s) 39, 266, 698, 800
PspGI CCWGG 4 cut(s) 39, 266, 698, 800
PspN4I GGNNCC 5 cut(s) 94, 184, 265, 704, 844
PspPI GGNCC 3 cut(s) 92, 182, 540
PstNI CAGNNNCTG 1 cut(s) 944
PvuII CAGCTG 1 cut(s) 532
RsaI GTAC 2 cut(s) 179, 979
RsaNI GTAC 2 cut(s) 178, 978
RseI CAYNNNNRTG 1 cut(s) 74
SacI GAGCTC 1 cut(s) 426
SalI GTCGAC 1 cut(s) 140
SapI GCTCTTC 1 cut(s) 490
SaqAI TTAA 3 cut(s) 864, 876, 948
SatI GCNGC 3 cut(s) 67, 530, 762
Sau3AI GATC 3 cut(s) 117, 364, 754
Sau96I GGNCC 3 cut(s) 92, 182, 540
ScaI AGTACT 1 cut(s) 979
SchI GAGTC 2 cut(s) 137, 991
ScrFI CCNGG 5 cut(s) 41, 268, 700, 802, 840
SduI GDGCHC 3 cut(s) 426, 502, 833
SfaNI GCATC 3 cut(s) 485, 786, 931
SfuI TTCGAA 1 cut(s) 685
SinI GGWCC 2 cut(s) 182, 540
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 2 cut(s) 54, 425
SmoI CTYRAG 2 cut(s) 54, 425
Sse9I AATT 4 cut(s) 245, 257, 653, 859
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 3 cut(s) 5, 275, 936
SspI AATATT 1 cut(s) 738
SspMI CTAG 5 cut(s) 434, 623, 719, 791, 969
SstI GAGCTC 1 cut(s) 426
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 5 cut(s) 39, 266, 698, 800, 838
StyI CCWWGG 1 cut(s) 814
TaaI ACNGT 3 cut(s) 182, 415, 617
TaqI TCGA 5 cut(s) 141, 162, 295, 660, 685
TasI AATT 4 cut(s) 245, 257, 653, 859
TatI WGTACW 1 cut(s) 977
TfiI GAWTC 4 cut(s) 292, 356, 633, 923
Tru1I TTAA 3 cut(s) 864, 876, 948
Tru9I TTAA 3 cut(s) 864, 876, 948
TscAI CASTG 1 cut(s) 801
TseFI GTSAC 3 cut(s) 30, 151, 409
TseI GCWGC 3 cut(s) 66, 529, 761
Tsp45I GTSAC 3 cut(s) 30, 151, 409
TspDTI ATGAA 4 cut(s) 35, 500, 797, 1002
TspRI CASTG 1 cut(s) 801
VpaK11BI GGWCC 2 cut(s) 182, 540
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 3 cut(s) 257, 653, 859
XceI RCATGY 2 cut(s) 79, 178
XcmI CCANNNNNNNNNTGG 1 cut(s) 1011
XmiI GTMKAC 1 cut(s) 141
XmnI GAANNNNTTC 1 cut(s) 666
XspI CTAG 5 cut(s) 434, 623, 719, 791, 969
ZrmI AGTACT 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.