Rroxscaffold_5G00355710

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000435
Physical Location & Seq
Forward (+)
34579300 .. 34584063
4764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00355710.1

Sequence Viewer

Length: 990 bp
ATGGCGAGCATGAGGAGGATGAAGCTCGGGTCACAAGGCCTGGAGGTCTCAGCACAAGGACTGGGCTGCATGGGCATGTCTGCCTTCTATGGCGCCCCGAAACCCGAACCCGACATGATCAATCTCATCCACCACGCCATCGACTCCGGCGTCACCTTCCTCGACACCGCCGACCTGTACGGTCCCCACACCAACGAAATTCTCCTCGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAACTGGCCACCAAATTCGGTATCAGGTTTGCGGACAACAAAATGGAGGTTCAAGGCGACCCGGCATATGTGAGAGCTGCTTGTGAAGAAAGCTTGAAACGCCTTGGTGTTGCTTCTGTTGATCTCTGTTATCAGATTAGGATTGATACCCGTGTCCCCATTGAAGTCACGCCTCAGCTGCTTCGTATTGAATTGATCAAGTTTGAAGAGGTTGAGAGTGCTGAGTGTAAATGGGCCTTCACAAATCTTGCTTTAGTTGCATTGCGTCAAGTGGGAGAGCTCAAGAAGCTAATTGAAGAGGGTAAAATAAAGTACATCGGTCTGTCTGAGGCGTCAGCTTCAACAATAAGAAGAGCCCATGCTGTTCATCCAATAACTGCTGTGCAGCTGGAGTGGTCCTTGTGGTCAAGAGATGTAGAGGAAGAAATAATCCCTACTTGTCGGGAACTTGACATTGGCATTGTTGCATACAGTCCTCTAGGAAGAGGATTCTTATCATCGGGGGCAAATTTCATCGAAAATCTTCCCAATGATGATGCTCGAAAGAATATGCCCAGGTTCCAAGCTGAAAACCTAGAGCATAATAAAACAATATTTCAGCGAGTAAGTGATCTTGCAGCAAGGAAGGGTTGCACCCCATCTCAACTAGCACCGGCCTGGGTTCATCACCAAGGAAATGATGTGTGCCCTATACCTGGAACCACCAAGATTGAGAATTTTAACCAGAACATTAAGGCTCGTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

329

Amino Acids

36.38

Weight (kDa)

6.27

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 327 1.7e-60 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10810 AT1G10810 AT1G60680 AT1G60690 AT1G60710 AT1G60730 AT1G60730 AT1G60750
fragaria_vesca FvH4_4g12490
malus_domestica MD04G1026100.v1.1 MD09G1032900.v1.1 MD13G1279900.v1.1
prunus_persica Prupe.1G175500_v2.0.a1 Prupe.1G175600_v2.0.a1 Prupe.1G175700_v2.0.a1 Prupe.1G175800_v2.0.a1 Prupe.2G050800_v2.0.a1
pyrus_communis pycom04g02210 pycom04g02230 pycom111g02620 pycom13g29370
rosa_chinensis RchiOBHm_Chr4g0409691 RchiOBHm_Chr4g0410671 RchiOBHm_Chr4g0410821 RchiOBHm_Chr4g0411011 RchiOBHm_Chr4g0411051 RchiOBHm_Chr4g0411101 RchiOBHm_Chr4g0411151 RchiOBHm_Chr4g0411171 RchiOBHm_Chr4g0411201
rosa_laevigata RLG00000008369 RLG00000008372 RLG00000008374 RLG00000008376 RLG00000008378 RLG00000008381 RLG00000008383 RLG00000008395
rosa_multiflora Rmu_co8497103.1_g000001 Rmu_co8514481.1_g000001 Rmu_sc0000543.1_g000005 Rmu_sc0000543.1_g000006 Rmu_sc0000543.1_g000024 Rmu_sc0000944.1_g000002 Rmu_sc0000944.1_g000011 Rmu_sc0001244.1_g000006 Rmu_sc0001244.1_g000014 Rmu_sc0001244.1_g000017 Rmu_sc0001244.1_g000020 Rmu_sc0003150.1_g000001 Rmu_sc0007879.1_g000001 Rmu_sc0007879.1_g000007 Rmu_sc0009499.1_g000008 Rmu_sc0029727.1_g000001
rosa_roxburghii Rroxscaffold_5G00354230 Rroxscaffold_5G00355560 Rroxscaffold_5G00355580 Rroxscaffold_5G00355600 Rroxscaffold_5G00355650 Rroxscaffold_5G00355710 Rroxscaffold_5G00355730 Rroxscaffold_5G00355760 Rroxscaffold_5G00355800 Rroxscaffold_5G00355840 Rroxscaffold_5G00355850
rosa_rugosa Rorug04G0096400 Rorug04G0105400 Rorug04G0110600 Rorug04G0110700 Rorug05G0412600 Rorug07G0180500
rosa_samantha Rh4AG157500 Rh4BG162500 Rh4BG163400 Rh4CG175300 Rh4CG176100 Rh4CG177100 Rh4CG177200 Rh4CG177400 Rh4CG177500 Rh4CG178000 Rh4CG178100 Rh4CG178300 Rh4DG159900 Rh4DG160200
rosa_wichuraiana Rw4G013600 Rw4G013610 Rw4G013660 Rw4G013690 Rw4G013700 Rw4G013710 Rw4G013720 Rw4G013730 Rw4G013740 Rw4G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AccB1I GGYRCC 1 cut(s) 92
AciI CCGC 2 cut(s) 168, 275
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 4 cut(s) 198, 257, 751, 958
AcyI GRCGYC 3 cut(s) 93, 150, 575
AfaI GTAC 2 cut(s) 179, 557
AfiI CCNNNNNNNGG 2 cut(s) 211, 938
AgsI TTSAA 8 cut(s) 245, 296, 340, 407, 434, 449, 539, 585
AjnI CCWGG 4 cut(s) 39, 797, 899, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 681, 713
AluBI AGCT 9 cut(s) 25, 320, 336, 421, 523, 532, 581, 631, 809
AluI AGCT 9 cut(s) 25, 320, 336, 421, 523, 532, 581, 631, 809
Alw21I GWGCWC 1 cut(s) 525
Alw26I GTCTC 2 cut(s) 52, 228
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 4 cut(s) 37, 249, 477, 897
ApeKI GCWGC 5 cut(s) 66, 320, 421, 628, 860
ApoI RAATTY 4 cut(s) 198, 257, 751, 958
Asp700I GAANNNNTTC 1 cut(s) 765
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 3 cut(s) 182, 477, 639
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 3 cut(s) 145, 241, 902
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 2 cut(s) 182, 639
BaeGI GKGCMC 1 cut(s) 932
BaeI ACNNNNGTAYC 2 cut(s) 381, 414
BalI TGGCCA 1 cut(s) 251
BanI GGYRCC 1 cut(s) 92
BanII GRGCYC 2 cut(s) 525, 601
Bbv12I GWGCWC 1 cut(s) 525
BbvCI CCTCAGC 1 cut(s) 417
BbvI GCAGC 5 cut(s) 53, 307, 408, 640, 872
BccI CCATC 2 cut(s) 146, 889
BciT130I CCWGG 4 cut(s) 41, 799, 901, 939
BclI TGATCA 2 cut(s) 117, 438
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 2 cut(s) 52, 228
BfaI CTAG 3 cut(s) 722, 818, 890
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 5 cut(s) 67, 321, 422, 629, 861
BlsI GCNGC 5 cut(s) 68, 322, 423, 630, 862
Bme1390I CCNGG 5 cut(s) 41, 305, 799, 901, 939
Bme18I GGWCC 2 cut(s) 182, 639
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 3 cut(s) 182, 477, 639
BmiI GGNNCC 4 cut(s) 94, 184, 803, 943
BmrFI CCNGG 5 cut(s) 41, 305, 799, 901, 939
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 1 cut(s) 769
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 2 cut(s) 62, 653
Bpu10I CCTNAGC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 509
BpuMI CCSGG 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 736
BsaHI GRCGYC 3 cut(s) 93, 150, 575
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 5 cut(s) 205, 346, 797, 900, 913
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 938
Bse118I RCCGGY 1 cut(s) 895
Bse1I ACTGG 2 cut(s) 66, 252
Bse3DI GCAATG 1 cut(s) 503
Bse8I GATNNNNATC 1 cut(s) 736
BseBI CCWGG 4 cut(s) 41, 799, 901, 939
BseDI CCNNGG 5 cut(s) 205, 346, 797, 900, 913
BseGI GGATG 3 cut(s) 24, 126, 610
BseJI GATNNNNATC 1 cut(s) 736
BseLI CCNNNNNNNGG 2 cut(s) 211, 938
BseMI GCAATG 1 cut(s) 503
BseMII CTCAG 4 cut(s) 63, 431, 456, 561
BseNI ACTGG 2 cut(s) 66, 252
BseRI GAGGAG 2 cut(s) 28, 194
BseSI GKGCMC 1 cut(s) 932
BseXI GCAGC 5 cut(s) 53, 307, 408, 640, 872
BsgI GTGCAG 1 cut(s) 647
BshFI GGCC 4 cut(s) 39, 251, 479, 899
BshNI GGYRCC 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 525
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 3 cut(s) 147, 305, 896
BslFI GGGAC 2 cut(s) 168, 383
BslI CCNNNNNNNGG 2 cut(s) 211, 938
BsmAI GTCTC 2 cut(s) 52, 228
BsmFI GGGAC 2 cut(s) 168, 383
BsnI GGCC 4 cut(s) 39, 251, 479, 899
Bso31I GGTCTC 1 cut(s) 52
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 3 cut(s) 525, 601, 932
Bsp143I GATC 4 cut(s) 117, 364, 438, 853
BspACI CCGC 2 cut(s) 168, 275
BspANI GGCC 4 cut(s) 39, 251, 479, 899
BspCNI CTCAG 4 cut(s) 62, 430, 457, 562
BspLI GGNNCC 4 cut(s) 94, 184, 803, 943
BspQI GCTCTTC 1 cut(s) 589
BspT107I GGYRCC 1 cut(s) 92
BspTNI GGTCTC 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 503
BsrFI RCCGGY 1 cut(s) 895
BsrI ACTGG 2 cut(s) 66, 252
BssAI RCCGGY 1 cut(s) 895
BssECI CCNNGG 5 cut(s) 205, 346, 797, 900, 913
BssMI GATC 4 cut(s) 117, 364, 438, 853
BssNI GRCGYC 3 cut(s) 93, 150, 575
BssT1I CCWWGG 2 cut(s) 346, 913
Bst2UI CCWGG 4 cut(s) 41, 799, 901, 939
Bst4CI ACNGT 2 cut(s) 182, 716
Bst6I CTCTTC 4 cut(s) 444, 534, 589, 721
BstACI GRCGYC 3 cut(s) 93, 150, 575
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 4 cut(s) 49, 417, 465, 570
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 3 cut(s) 24, 126, 610
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 4 cut(s) 120, 367, 441, 856
BstMAI GTCTC 2 cut(s) 52, 228
BstMBI GATC 4 cut(s) 117, 364, 438, 853
BstMWI GCNNNNNNNGC 5 cut(s) 72, 342, 421, 500, 529
BstNI CCWGG 4 cut(s) 41, 799, 901, 939
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 5 cut(s) 39, 303, 797, 899, 937
BstSLI GKGCMC 1 cut(s) 932
BstV1I GCAGC 5 cut(s) 53, 307, 408, 640, 872
BsuRI GGCC 4 cut(s) 39, 251, 479, 899
BtsCI GGATG 3 cut(s) 24, 126, 610
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 895
Cfr13I GGNCC 3 cut(s) 182, 477, 639
CseI GACGC 3 cut(s) 139, 497, 564
Csp6I GTAC 2 cut(s) 178, 556
CspCI CAANNNNNGTGG 2 cut(s) 934, 969
CviAII CATG 5 cut(s) 10, 70, 76, 115, 602
CviQI GTAC 2 cut(s) 178, 556
DdeI CTNAG 4 cut(s) 49, 417, 465, 570
DinI GGCGCC 1 cut(s) 94
DpnI GATC 4 cut(s) 119, 366, 440, 855
DpnII GATC 4 cut(s) 117, 364, 438, 853
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 4 cut(s) 444, 534, 589, 721
EarI CTCTTC 4 cut(s) 444, 534, 589, 721
Ecl136II GAGCTC 1 cut(s) 523
Eco130I CCWWGG 2 cut(s) 346, 913
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 2 cut(s) 525, 601
Eco31I GGTCTC 1 cut(s) 52
Eco47I GGWCC 2 cut(s) 182, 639
Eco53kI GAGCTC 1 cut(s) 523
Eco88I CYCGRG 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 523
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 4 cut(s) 39, 797, 899, 937
EcoT14I CCWWGG 2 cut(s) 346, 913
EcoT38I GRGCYC 2 cut(s) 525, 601
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 2 cut(s) 346, 913
FaeI CATG 5 cut(s) 13, 73, 79, 118, 605
FaqI GGGAC 2 cut(s) 168, 383
FatI CATG 5 cut(s) 9, 69, 75, 114, 601
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 2 cut(s) 117, 438
Fnu4HI GCNGC 5 cut(s) 67, 321, 422, 629, 861
FokI GGATG 3 cut(s) 31, 113, 597
FriOI GRGCYC 2 cut(s) 525, 601
Fsp4HI GCNGC 5 cut(s) 67, 321, 422, 629, 861
FspBI CTAG 3 cut(s) 722, 818, 890
GlaI GCGC 1 cut(s) 94
GluI GCNGC 5 cut(s) 67, 321, 422, 629, 861
GsuI CTGGAG 2 cut(s) 62, 653
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 39, 251, 479, 899
HapII CCGG 3 cut(s) 147, 305, 896
HgaI GACGC 3 cut(s) 139, 497, 564
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 3 cut(s) 93, 150, 575
Hin1II CATG 5 cut(s) 13, 73, 79, 118, 605
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 2 cut(s) 143, 732
HpaII CCGG 3 cut(s) 147, 305, 896
HphI GGTGA 3 cut(s) 145, 241, 902
Hpy188I TCNGA 2 cut(s) 378, 571
Hpy188III TCNNGA 3 cut(s) 526, 651, 686
HpyAV CCTTC 4 cut(s) 94, 166, 490, 862
HpyCH4III ACNGT 2 cut(s) 182, 716
HpyCH4V TGCA 6 cut(s) 69, 503, 628, 710, 860, 876
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 342, 421, 500, 529
HpyF3I CTNAG 4 cut(s) 49, 417, 465, 570
Hsp92I GRCGYC 3 cut(s) 93, 150, 575
Hsp92II CATG 5 cut(s) 13, 73, 79, 118, 605
HspAI GCGC 1 cut(s) 93
KasI GGCGCC 1 cut(s) 92
Ksp22I TGATCA 2 cut(s) 117, 438
Kzo9I GATC 4 cut(s) 117, 364, 438, 853
LguI GCTCTTC 1 cut(s) 589
Lsp1109I GCAGC 5 cut(s) 53, 307, 408, 640, 872
LweI GCATC 1 cut(s) 769
MaeI CTAG 3 cut(s) 722, 818, 890
MaeIII GTNAC 3 cut(s) 30, 151, 409
MalI GATC 4 cut(s) 119, 366, 440, 855
MboI GATC 4 cut(s) 117, 364, 438, 853
MboII GAAGA 7 cut(s) 341, 461, 551, 606, 677, 738, 758
MhlI GDGCHC 3 cut(s) 525, 601, 932
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 6 cut(s) 198, 257, 434, 534, 751, 958
MluNI TGGCCA 1 cut(s) 251
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 1 cut(s) 137
Mox20I TGGCCA 1 cut(s) 251
MroXI GAANNNNTTC 1 cut(s) 765
MscI TGGCCA 1 cut(s) 251
MseI TTAA 2 cut(s) 963, 975
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 2 cut(s) 421, 631
MspI CCGG 3 cut(s) 147, 305, 896
MspR9I CCNGG 5 cut(s) 41, 305, 799, 901, 939
MvaI CCWGG 4 cut(s) 41, 799, 901, 939
MwoI GCNNNNNNNGC 5 cut(s) 72, 342, 421, 500, 529
NarI GGCGCC 1 cut(s) 93
NciI CCSGG 1 cut(s) 305
NdeI CATATG 1 cut(s) 310
NdeII GATC 4 cut(s) 117, 364, 438, 853
NlaIII CATG 5 cut(s) 13, 73, 79, 118, 605
NlaIV GGNNCC 4 cut(s) 94, 184, 803, 943
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 3 cut(s) 30, 151, 409
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 589
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PdmI GAANNNNTTC 1 cut(s) 765
PfeI GAWTC 1 cut(s) 732
PkrI GCNGC 5 cut(s) 68, 322, 423, 630, 862
PleI GAGTC 1 cut(s) 137
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 525
Psp6I CCWGG 4 cut(s) 39, 797, 899, 937
PspGI CCWGG 4 cut(s) 39, 797, 899, 937
PspN4I GGNNCC 4 cut(s) 94, 184, 803, 943
PspPI GGNCC 3 cut(s) 182, 477, 639
PvuII CAGCTG 2 cut(s) 421, 631
RsaI GTAC 2 cut(s) 179, 557
RsaNI GTAC 2 cut(s) 178, 556
RseI CAYNNNNRTG 1 cut(s) 74
SacI GAGCTC 1 cut(s) 525
SapI GCTCTTC 1 cut(s) 589
SaqAI TTAA 2 cut(s) 963, 975
SatI GCNGC 5 cut(s) 67, 321, 422, 629, 861
Sau3AI GATC 4 cut(s) 117, 364, 438, 853
Sau96I GGNCC 3 cut(s) 182, 477, 639
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 5 cut(s) 41, 305, 799, 901, 939
SduI GDGCHC 3 cut(s) 525, 601, 932
SfaNI GCATC 1 cut(s) 769
SfoI GGCGCC 1 cut(s) 94
SinI GGWCC 2 cut(s) 182, 639
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 6 cut(s) 198, 257, 434, 534, 751, 958
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 2 cut(s) 168, 275
SspDI GGCGCC 1 cut(s) 92
SspI AATATT 1 cut(s) 837
SspMI CTAG 3 cut(s) 722, 818, 890
SstI GAGCTC 1 cut(s) 525
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 5 cut(s) 39, 303, 797, 899, 937
StyI CCWWGG 2 cut(s) 346, 913
TaaI ACNGT 2 cut(s) 182, 716
TaqI TCGA 4 cut(s) 141, 162, 759, 784
TaqII GACCGA 1 cut(s) 551
TasI AATT 6 cut(s) 198, 257, 434, 534, 751, 958
TatI WGTACW 1 cut(s) 555
TfiI GAWTC 1 cut(s) 732
Tru1I TTAA 2 cut(s) 963, 975
Tru9I TTAA 2 cut(s) 963, 975
TseFI GTSAC 3 cut(s) 30, 151, 409
TseI GCWGC 5 cut(s) 66, 320, 421, 628, 860
Tsp45I GTSAC 3 cut(s) 30, 151, 409
TspDTI ATGAA 4 cut(s) 35, 599, 745, 896
TspGWI ACGGA 1 cut(s) 975
VpaK11BI GGWCC 2 cut(s) 182, 639
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 4 cut(s) 198, 257, 751, 958
XceI RCATGY 1 cut(s) 79
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 3 cut(s) 722, 818, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.