Rroxscaffold_5G00355800

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000435
Physical Location & Seq
Forward (+)
34826816 .. 34830204
3389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00355800.1

Sequence Viewer

Length: 1104 bp
ATGCACCTTCCTTCACTCACTCTGCTACTACCTGCTTCTGCTCACTCACTCACAGAGAGACATTTAACACATAGACATGGCGGGTGTGAGGAGGATGAAGGTCGGGTCACAAGGCCTGGAGGTGTCAGCTCAAGGACTGGGCTGCATGGGCATGTCAGCCTTCTATGGCCCCCGAAGCCCGAAGCCGACATGATCAAGCTCATCCACCATGCTGTCGACTCCGGCGTCACCTTCCTTGACACTTCCGACGTCTACGGTCCCCACACCAACGAAATTCTCCTCGGCAAGGCTTTGAATGGAGGGGTGAGAGACAAGGTTGAACTGGCCACCAAATGCGGTATCAGGTTTGCGGACAACAAAATGGAGGTTCAAGGCGACCCGGCATATGTGAGAGCTGCTTGTGAAGGAAGCTTGAAACGCCTTGGTGTTGCTTCTGTTGATCTCTATTATCAGCATAGGATTGACACCCGTGTCCCCATTGAAGTCACGGTGGGGGAGCTCAAGAAGCTAATTGAAGAGGGTAAAATAAAGTACATCGGCCTGTCTGAGGCGTCAGCTTCCACAATAAGAAGAGCCCATGCTGTTCATCCAATAACTGCTGTGCAGCTGGAGTGGTCCTTGTGGTCAAGAGATGTAGAGGAAGAAATAATCCCTACTTGTCGGGAACTTGGCATTGGCATTGTTGCATACAGTCCTCTAGGAAGAGGATTCTTATCATCGGGGGCAAATATCATCGAAAATCTTTCCAATGATGATTCTCGAAAGGATATGCCCAGGTTCCAAGCCGACAACCTAGAGCATAATAAAACAATATTTGAGCGAGTAAGTGATCTTGCAGCAAGGAAGGGTTGCACCCCATCTCAACTAGCACTGGCCTGGGTTCATCACCAAGGAAATGATGTGTGCCCTATACCCGGAACCACCAAGATTGAGAATTTTAACCAGAACATTAAGGCTTTGTCTGTGAAACTGACACCAGAAGAAATGGCCGAGGTTGAATCTTTTGCTTCTGCAGACGCTGTTAAGGGCGACAGATATCAAACTGGCTTCAGCACTTGGATGAACTCTGAAACTCCAACACTGTCTTCATGGAAAGCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

367

Amino Acids

40.31

Weight (kDa)

6.15

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 56 - 335 4e-73 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10810 AT1G10810 AT1G60680 AT1G60690 AT1G60710 AT1G60730 AT1G60730 AT1G60750
fragaria_vesca FvH4_4g12490
malus_domestica MD04G1026100.v1.1 MD09G1032900.v1.1 MD13G1279900.v1.1
prunus_persica Prupe.1G175500_v2.0.a1 Prupe.1G175600_v2.0.a1 Prupe.1G175700_v2.0.a1 Prupe.1G175800_v2.0.a1 Prupe.2G050800_v2.0.a1
pyrus_communis pycom04g02210 pycom04g02230 pycom111g02620 pycom13g29370
rosa_chinensis RchiOBHm_Chr4g0409691 RchiOBHm_Chr4g0410671 RchiOBHm_Chr4g0410821 RchiOBHm_Chr4g0411011 RchiOBHm_Chr4g0411051 RchiOBHm_Chr4g0411101 RchiOBHm_Chr4g0411151 RchiOBHm_Chr4g0411171 RchiOBHm_Chr4g0411201
rosa_laevigata RLG00000008369 RLG00000008372 RLG00000008374 RLG00000008376 RLG00000008378 RLG00000008381 RLG00000008383 RLG00000008395
rosa_multiflora Rmu_co8497103.1_g000001 Rmu_co8514481.1_g000001 Rmu_sc0000543.1_g000005 Rmu_sc0000543.1_g000006 Rmu_sc0000543.1_g000024 Rmu_sc0000944.1_g000002 Rmu_sc0000944.1_g000011 Rmu_sc0001244.1_g000006 Rmu_sc0001244.1_g000014 Rmu_sc0001244.1_g000017 Rmu_sc0001244.1_g000020 Rmu_sc0003150.1_g000001 Rmu_sc0007879.1_g000001 Rmu_sc0007879.1_g000007 Rmu_sc0009499.1_g000008 Rmu_sc0029727.1_g000001
rosa_roxburghii Rroxscaffold_5G00354230 Rroxscaffold_5G00355560 Rroxscaffold_5G00355580 Rroxscaffold_5G00355600 Rroxscaffold_5G00355650 Rroxscaffold_5G00355710 Rroxscaffold_5G00355730 Rroxscaffold_5G00355760 Rroxscaffold_5G00355800 Rroxscaffold_5G00355840 Rroxscaffold_5G00355850
rosa_rugosa Rorug04G0096400 Rorug04G0105400 Rorug04G0110600 Rorug04G0110700 Rorug05G0412600 Rorug07G0180500
rosa_samantha Rh4AG157500 Rh4BG162500 Rh4BG163400 Rh4CG175300 Rh4CG176100 Rh4CG177100 Rh4CG177200 Rh4CG177400 Rh4CG177500 Rh4CG178000 Rh4CG178100 Rh4CG178300 Rh4DG159900 Rh4DG160200
rosa_wichuraiana Rw4G013600 Rw4G013610 Rw4G013660 Rw4G013690 Rw4G013700 Rw4G013710 Rw4G013720 Rw4G013730 Rw4G013740 Rw4G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 224, 470
AatII GACGTC 1 cut(s) 252
Acc36I ACCTGC 1 cut(s) 40
AccI GTMKAC 2 cut(s) 216, 252
AciI CCGC 3 cut(s) 81, 336, 350
AcoI YGGCCR 2 cut(s) 324, 987
AcsI RAATTY 2 cut(s) 273, 934
AcuI CTGAAG 1 cut(s) 1033
AcyI GRCGYC 3 cut(s) 225, 249, 551
AfaI GTAC 1 cut(s) 533
AfiI CCNNNNNNNGG 3 cut(s) 286, 547, 914
AgsI TTSAA 7 cut(s) 295, 320, 371, 415, 482, 515, 998
AjnI CCWGG 3 cut(s) 115, 773, 875
AjuI GAANNNNNNNTTGG 4 cut(s) 657, 689, 1069, 1101
AluBI AGCT 8 cut(s) 129, 199, 395, 411, 499, 508, 557, 607
AluI AGCT 8 cut(s) 129, 199, 395, 411, 499, 508, 557, 607
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 2 cut(s) 52, 303
AlwNI CAGNNNCTG 1 cut(s) 1019
AoxI GGCC 6 cut(s) 113, 167, 324, 538, 873, 987
ApeKI GCWGC 4 cut(s) 142, 395, 604, 836
ApoI RAATTY 2 cut(s) 273, 934
AspS9I GGNCC 3 cut(s) 168, 257, 615
AsuC2I CCSGG 2 cut(s) 380, 915
AsuHPI GGTGA 3 cut(s) 220, 316, 878
AvaII GGWCC 2 cut(s) 257, 615
BaeGI GKGCMC 1 cut(s) 908
BalI TGGCCA 1 cut(s) 326
BanII GRGCYC 2 cut(s) 501, 577
BbsI GAAGAC 1 cut(s) 1077
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 4 cut(s) 129, 382, 616, 848
BccI CCATC 1 cut(s) 865
BciT130I CCWGG 3 cut(s) 117, 775, 877
BclI TGATCA 1 cut(s) 192
BcnI CCSGG 2 cut(s) 380, 915
BcoDI GTCTC 2 cut(s) 52, 303
BfaI CTAG 3 cut(s) 698, 794, 866
BfmI CTRYAG 1 cut(s) 1011
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 4 cut(s) 143, 396, 605, 837
BlsI GCNGC 4 cut(s) 144, 397, 606, 838
Bme1390I CCNGG 5 cut(s) 117, 380, 775, 877, 915
Bme18I GGWCC 2 cut(s) 257, 615
BmgT120I GGNCC 3 cut(s) 168, 257, 615
BmiI GGNNCC 4 cut(s) 170, 259, 779, 919
BmrFI CCNGG 5 cut(s) 117, 380, 775, 877, 915
BmrI ACTGGG 1 cut(s) 147
BmuI ACTGGG 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 1077
BpmI CTGGAG 2 cut(s) 138, 629
BpuEI CTTGAG 2 cut(s) 115, 485
BpuMI CCSGG 2 cut(s) 380, 915
BsaBI GATNNNNATC 1 cut(s) 712
BsaHI GRCGYC 3 cut(s) 225, 249, 551
BsaJI CCNNGG 6 cut(s) 280, 421, 773, 876, 889, 990
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 547, 914
Bse1I ACTGG 4 cut(s) 142, 327, 876, 1048
Bse8I GATNNNNATC 1 cut(s) 712
BseBI CCWGG 3 cut(s) 117, 775, 877
BseDI CCNNGG 6 cut(s) 280, 421, 773, 876, 889, 990
BseGI GGATG 4 cut(s) 100, 201, 586, 1065
BseJI GATNNNNATC 1 cut(s) 712
BseLI CCNNNNNNNGG 3 cut(s) 286, 547, 914
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 4 cut(s) 142, 327, 876, 1048
BseRI GAGGAG 2 cut(s) 104, 269
BseSI GKGCMC 1 cut(s) 908
BseXI GCAGC 4 cut(s) 129, 382, 616, 848
BsgI GTGCAG 1 cut(s) 623
BshFI GGCC 6 cut(s) 115, 169, 326, 540, 875, 989
BsiHKAI GWGCWC 1 cut(s) 501
BsiSI CCGG 3 cut(s) 222, 380, 915
BslFI GGGAC 2 cut(s) 243, 458
BslI CCNNNNNNNGG 3 cut(s) 286, 547, 914
BsmAI GTCTC 2 cut(s) 52, 303
BsmFI GGGAC 2 cut(s) 243, 458
BsnI GGCC 6 cut(s) 115, 169, 326, 540, 875, 989
Bsp1286I GDGCHC 3 cut(s) 501, 577, 908
Bsp143I GATC 3 cut(s) 192, 439, 829
BspACI CCGC 3 cut(s) 81, 336, 350
BspANI GGCC 6 cut(s) 115, 169, 326, 540, 875, 989
BspCNI CTCAG 1 cut(s) 538
BspLI GGNNCC 4 cut(s) 170, 259, 779, 919
BspMAI CTGCAG 1 cut(s) 1015
BspMI ACCTGC 1 cut(s) 40
BspQI GCTCTTC 1 cut(s) 565
BsrI ACTGG 4 cut(s) 142, 327, 876, 1048
BssECI CCNNGG 6 cut(s) 280, 421, 773, 876, 889, 990
BssMI GATC 3 cut(s) 192, 439, 829
BssNI GRCGYC 3 cut(s) 225, 249, 551
BssT1I CCWWGG 2 cut(s) 421, 889
Bst2UI CCWGG 3 cut(s) 117, 775, 877
Bst4CI ACNGT 4 cut(s) 257, 490, 692, 1083
Bst6I CTCTTC 3 cut(s) 510, 565, 697
BstACI GRCGYC 3 cut(s) 225, 249, 551
BstDEI CTNAG 1 cut(s) 546
BstENI CCTNNNNNAGG 2 cut(s) 284, 545
BstF5I GGATG 4 cut(s) 100, 201, 586, 1065
BstKTI GATC 3 cut(s) 195, 442, 832
BstMAI GTCTC 2 cut(s) 52, 303
BstMBI GATC 3 cut(s) 192, 439, 829
BstMWI GCNNNNNNNGC 4 cut(s) 148, 175, 417, 505
BstNI CCWGG 3 cut(s) 117, 775, 877
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 5 cut(s) 115, 378, 773, 875, 913
BstSFI CTRYAG 1 cut(s) 1011
BstSLI GKGCMC 1 cut(s) 908
BstV1I GCAGC 4 cut(s) 129, 382, 616, 848
BstV2I GAAGAC 1 cut(s) 1077
BsuRI GGCC 6 cut(s) 115, 169, 326, 540, 875, 989
BtsCI GGATG 4 cut(s) 100, 201, 586, 1065
BtsIMutI CAGTG 2 cut(s) 869, 1079
BveI ACCTGC 1 cut(s) 40
CaiI CAGNNNCTG 1 cut(s) 1019
Cfr13I GGNCC 3 cut(s) 168, 257, 615
CseI GACGC 3 cut(s) 214, 540, 1025
Csp6I GTAC 1 cut(s) 532
CspCI CAANNNNNGTGG 2 cut(s) 910, 945
CviAII CATG 7 cut(s) 77, 146, 152, 190, 209, 578, 1089
CviQI GTAC 1 cut(s) 532
DdeI CTNAG 1 cut(s) 546
DpnI GATC 3 cut(s) 194, 441, 831
DpnII GATC 3 cut(s) 192, 439, 829
DrdI GACNNNNNNGTC 2 cut(s) 224, 470
DseDI GACNNNNNNGTC 2 cut(s) 224, 470
EaeI YGGCCR 2 cut(s) 324, 987
Eam1104I CTCTTC 3 cut(s) 510, 565, 697
EarI CTCTTC 3 cut(s) 510, 565, 697
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 2 cut(s) 421, 889
Eco147I AGGCCT 1 cut(s) 115
Eco24I GRGCYC 2 cut(s) 501, 577
Eco32I GATATC 1 cut(s) 1037
Eco47I GGWCC 2 cut(s) 257, 615
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 1 cut(s) 1033
EcoICRI GAGCTC 1 cut(s) 499
EcoNI CCTNNNNNAGG 2 cut(s) 284, 545
EcoRII CCWGG 3 cut(s) 115, 773, 875
EcoRV GATATC 1 cut(s) 1037
EcoT14I CCWWGG 2 cut(s) 421, 889
EcoT38I GRGCYC 2 cut(s) 501, 577
ErhI CCWWGG 2 cut(s) 421, 889
FaeI CATG 7 cut(s) 80, 149, 155, 193, 212, 581, 1092
FaqI GGGAC 2 cut(s) 243, 458
FatI CATG 7 cut(s) 76, 145, 151, 189, 208, 577, 1088
FauI CCCGC 1 cut(s) 74
FauNDI CATATG 1 cut(s) 385
FbaI TGATCA 1 cut(s) 192
FblI GTMKAC 2 cut(s) 216, 252
Fnu4HI GCNGC 4 cut(s) 143, 396, 605, 837
FokI GGATG 4 cut(s) 107, 188, 573, 1072
FriOI GRGCYC 2 cut(s) 501, 577
Fsp4HI GCNGC 4 cut(s) 143, 396, 605, 837
FspBI CTAG 3 cut(s) 698, 794, 866
GluI GCNGC 4 cut(s) 143, 396, 605, 837
GsuI CTGGAG 2 cut(s) 138, 629
HaeIII GGCC 6 cut(s) 115, 169, 326, 540, 875, 989
HapII CCGG 3 cut(s) 222, 380, 915
HgaI GACGC 3 cut(s) 214, 540, 1025
Hin1I GRCGYC 3 cut(s) 225, 249, 551
Hin1II CATG 7 cut(s) 80, 149, 155, 193, 212, 581, 1092
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 409
HinfI GANTC 4 cut(s) 218, 708, 755, 998
HpaII CCGG 3 cut(s) 222, 380, 915
HphI GGTGA 3 cut(s) 220, 316, 878
Hpy166II GTNNAC 2 cut(s) 217, 253
Hpy188I TCNGA 3 cut(s) 247, 547, 1069
Hpy188III TCNNGA 4 cut(s) 502, 627, 662, 759
Hpy8I GTNNAC 2 cut(s) 217, 253
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 7 cut(s) 17, 21, 92, 170, 241, 398, 838
HpyCH4III ACNGT 4 cut(s) 257, 490, 692, 1083
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 7 cut(s) 4, 145, 604, 686, 836, 852, 1013
HpyF10VI GCNNNNNNNGC 4 cut(s) 148, 175, 417, 505
HpyF3I CTNAG 1 cut(s) 546
HpySE526I ACGT 1 cut(s) 249
Hsp92I GRCGYC 3 cut(s) 225, 249, 551
Hsp92II CATG 7 cut(s) 80, 149, 155, 193, 212, 581, 1092
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 3 cut(s) 192, 439, 829
LguI GCTCTTC 1 cut(s) 565
LmnI GCTCC 1 cut(s) 496
Lsp1109I GCAGC 4 cut(s) 129, 382, 616, 848
MaeI CTAG 3 cut(s) 698, 794, 866
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 3 cut(s) 106, 226, 484
MalI GATC 3 cut(s) 194, 441, 831
MboI GATC 3 cut(s) 192, 439, 829
MboII GAAGA 6 cut(s) 527, 582, 653, 714, 992, 1077
MhlI GDGCHC 3 cut(s) 501, 577, 908
MlsI TGGCCA 1 cut(s) 326
MluCI AATT 3 cut(s) 273, 510, 934
MluNI TGGCCA 1 cut(s) 326
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 2 cut(s) 270, 1100
Mox20I TGGCCA 1 cut(s) 326
MscI TGGCCA 1 cut(s) 326
MseI TTAA 4 cut(s) 65, 939, 951, 1023
MslI CAYNNNNRTG 3 cut(s) 75, 150, 1058
Msp20I TGGCCA 1 cut(s) 326
MspA1I CMGCKG 1 cut(s) 607
MspI CCGG 3 cut(s) 222, 380, 915
MspR9I CCNGG 5 cut(s) 117, 380, 775, 877, 915
MvaI CCWGG 3 cut(s) 117, 775, 877
MwoI GCNNNNNNNGC 4 cut(s) 148, 175, 417, 505
NciI CCSGG 2 cut(s) 380, 915
NdeI CATATG 1 cut(s) 385
NdeII GATC 3 cut(s) 192, 439, 829
NlaIII CATG 7 cut(s) 80, 149, 155, 193, 212, 581, 1092
NlaIV GGNNCC 4 cut(s) 170, 259, 779, 919
NmeAIII GCCGAG 2 cut(s) 261, 1015
NmuCI GTSAC 3 cut(s) 106, 226, 484
NspI RCATGY 1 cut(s) 155
PceI AGGCCT 1 cut(s) 115
PciSI GCTCTTC 1 cut(s) 565
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 3 cut(s) 708, 755, 998
PkrI GCNGC 4 cut(s) 144, 397, 606, 838
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 501
Psp6I CCWGG 3 cut(s) 115, 773, 875
PspGI CCWGG 3 cut(s) 115, 773, 875
PspN4I GGNNCC 4 cut(s) 170, 259, 779, 919
PspPI GGNCC 3 cut(s) 168, 257, 615
PstI CTGCAG 1 cut(s) 1015
PstNI CAGNNNCTG 1 cut(s) 1019
PvuII CAGCTG 1 cut(s) 607
RsaI GTAC 1 cut(s) 533
RsaNI GTAC 1 cut(s) 532
RseI CAYNNNNRTG 3 cut(s) 75, 150, 1058
SacI GAGCTC 1 cut(s) 501
SalI GTCGAC 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 565
SaqAI TTAA 4 cut(s) 65, 939, 951, 1023
SatI GCNGC 4 cut(s) 143, 396, 605, 837
Sau3AI GATC 3 cut(s) 192, 439, 829
Sau96I GGNCC 3 cut(s) 168, 257, 615
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 5 cut(s) 117, 380, 775, 877, 915
SduI GDGCHC 3 cut(s) 501, 577, 908
SfcI CTRYAG 1 cut(s) 1011
SinI GGWCC 2 cut(s) 257, 615
SmiMI CAYNNNNRTG 3 cut(s) 75, 150, 1058
SmlI CTYRAG 2 cut(s) 130, 500
SmoI CTYRAG 2 cut(s) 130, 500
Sse9I AATT 3 cut(s) 273, 510, 934
SseBI AGGCCT 1 cut(s) 115
SsiI CCGC 3 cut(s) 81, 336, 350
SspI AATATT 1 cut(s) 813
SspMI CTAG 3 cut(s) 698, 794, 866
SstI GAGCTC 1 cut(s) 501
StuI AGGCCT 1 cut(s) 115
StyD4I CCNGG 5 cut(s) 115, 378, 773, 875, 913
StyI CCWWGG 2 cut(s) 421, 889
TaaI ACNGT 4 cut(s) 257, 490, 692, 1083
TaiI ACGT 1 cut(s) 252
TaqI TCGA 3 cut(s) 216, 735, 760
TasI AATT 3 cut(s) 273, 510, 934
TatI WGTACW 1 cut(s) 531
TfiI GAWTC 3 cut(s) 708, 755, 998
Tru1I TTAA 4 cut(s) 65, 939, 951, 1023
Tru9I TTAA 4 cut(s) 65, 939, 951, 1023
TscAI CASTG 2 cut(s) 876, 1086
TseFI GTSAC 3 cut(s) 106, 226, 484
TseI GCWGC 4 cut(s) 142, 395, 604, 836
Tsp45I GTSAC 3 cut(s) 106, 226, 484
TspDTI ATGAA 5 cut(s) 111, 575, 872, 1076, 1077
TspRI CASTG 2 cut(s) 876, 1086
VpaK11BI GGWCC 2 cut(s) 257, 615
XagI CCTNNNNNAGG 2 cut(s) 284, 545
XapI RAATTY 2 cut(s) 273, 934
XceI RCATGY 1 cut(s) 155
XmiI GTMKAC 2 cut(s) 216, 252
XspI CTAG 3 cut(s) 698, 794, 866
ZraI GACGTC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.