Rroxscaffold_5G00354230

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000435
Physical Location & Seq
Forward (+)
32692008 .. 32705623
13616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00354230.1

Sequence Viewer

Length: 915 bp
ATGATCAAGCTCATCCACCATGCCGTTGACTCCGGCATCACCTTCCTCGACACCTCCGACAGCTATGGTCCACACACCAATGAAATTCTCCTCGGCAAGGTTTTGAGTGGAGGGGTGAGAGACAAGGTTGAATTGGCCACCAAATTCGGTATCAGGTTTGCGGACAACAAAATGGAGATTCGAGGCGACCCGACATATGTGAGAGCTGCTTGTGAAGCAAGCTTGAAGCGGCTCGATGTAGATTCTATTGATCTCTATTATCAGCATAGGATTGACACCCGTGTCCCCATTGAAGTCACGGTGGGGGAGCTCAAGAAGCTAATTGAAGAGGGTAAAATAAAGTACATTGGTCTGTCTGAGGCGTCAGCTTCAACAATAAGAAGAGCCCATGCTGTTCATCCAATAACTGCTGTGCAGCTGGAGTGGTCCTTGTGGTCAAGAGATGTAGAGGAAGAAATAATCCCTTCTTGTCGGGAACTTGGCATTGGCATTGTTGCATACAGTCCTCTAGGAAGAGGATTCTTATCATCGGGGGCAAATTTCATCGAAAATCTTCCCAATGATGATTATCGAAAGAATATGCCCAGGTTCCAAGCTGGAAACCTAGAGCATAATAAAACAATATTTGTGCGAGTAAGTGATCTTGCAGCAAGGAAGGGTTGCACCCCATCTCAACTAGCACTGGCCTGGGTTCATCACCAGGGAAATGATGTGTGTCCCATACCCGGAACCACCAAGATTGAGAATTTAAACCAGAATATTGGAGCTCTGTCTGTGAACCTCACATCAGAAGAATTGGCTGAGCTTGAATCCTTTGCTTCTACGGATTCTGTGAAGGGCGACCGATATCAAAATGGTATCAGTACTTGGATGAACTCTGAAACTCCACCACTGTCTTCATGGAAAGCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

304

Amino Acids

33.65

Weight (kDa)

5.88

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 2 - 272 1.9e-69 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10810 AT1G10810 AT1G60680 AT1G60690 AT1G60710 AT1G60730 AT1G60730 AT1G60750
fragaria_vesca FvH4_4g12490
malus_domestica MD04G1026100.v1.1 MD09G1032900.v1.1 MD13G1279900.v1.1
prunus_persica Prupe.1G175500_v2.0.a1 Prupe.1G175600_v2.0.a1 Prupe.1G175700_v2.0.a1 Prupe.1G175800_v2.0.a1 Prupe.2G050800_v2.0.a1
pyrus_communis pycom04g02210 pycom04g02230 pycom111g02620 pycom13g29370
rosa_chinensis RchiOBHm_Chr4g0409691 RchiOBHm_Chr4g0410671 RchiOBHm_Chr4g0410821 RchiOBHm_Chr4g0411011 RchiOBHm_Chr4g0411051 RchiOBHm_Chr4g0411101 RchiOBHm_Chr4g0411151 RchiOBHm_Chr4g0411171 RchiOBHm_Chr4g0411201
rosa_laevigata RLG00000008369 RLG00000008372 RLG00000008374 RLG00000008376 RLG00000008378 RLG00000008381 RLG00000008383 RLG00000008395
rosa_multiflora Rmu_co8497103.1_g000001 Rmu_co8514481.1_g000001 Rmu_sc0000543.1_g000005 Rmu_sc0000543.1_g000006 Rmu_sc0000543.1_g000024 Rmu_sc0000944.1_g000002 Rmu_sc0000944.1_g000011 Rmu_sc0001244.1_g000006 Rmu_sc0001244.1_g000014 Rmu_sc0001244.1_g000017 Rmu_sc0001244.1_g000020 Rmu_sc0003150.1_g000001 Rmu_sc0007879.1_g000001 Rmu_sc0007879.1_g000007 Rmu_sc0009499.1_g000008 Rmu_sc0029727.1_g000001
rosa_roxburghii Rroxscaffold_5G00354230 Rroxscaffold_5G00355560 Rroxscaffold_5G00355580 Rroxscaffold_5G00355600 Rroxscaffold_5G00355650 Rroxscaffold_5G00355710 Rroxscaffold_5G00355730 Rroxscaffold_5G00355760 Rroxscaffold_5G00355800 Rroxscaffold_5G00355840 Rroxscaffold_5G00355850
rosa_rugosa Rorug04G0096400 Rorug04G0105400 Rorug04G0110600 Rorug04G0110700 Rorug05G0412600 Rorug07G0180500
rosa_samantha Rh4AG157500 Rh4BG162500 Rh4BG163400 Rh4CG175300 Rh4CG176100 Rh4CG177100 Rh4CG177200 Rh4CG177400 Rh4CG177500 Rh4CG178000 Rh4CG178100 Rh4CG178300 Rh4DG159900 Rh4DG160200
rosa_wichuraiana Rw4G013600 Rw4G013610 Rw4G013660 Rw4G013690 Rw4G013700 Rw4G013710 Rw4G013720 Rw4G013730 Rw4G013740 Rw4G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 281
AciI CCGC 2 cut(s) 161, 229
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 4 cut(s) 84, 143, 538, 745
AcyI GRCGYC 1 cut(s) 362
AfaI GTAC 2 cut(s) 344, 865
AfiI CCNNNNNNNGG 2 cut(s) 97, 725
AgsI TTSAA 6 cut(s) 131, 226, 293, 326, 372, 809
AjnI CCWGG 3 cut(s) 584, 686, 699
AjuI GAANNNNNNNTTGG 2 cut(s) 468, 500
Alw21I GWGCWC 2 cut(s) 312, 769
Alw26I GTCTC 1 cut(s) 114
AoxI GGCC 2 cut(s) 135, 684
ApeKI GCWGC 3 cut(s) 206, 415, 647
ApoI RAATTY 4 cut(s) 84, 143, 538, 745
Asp700I GAANNNNTTC 1 cut(s) 552
AspS9I GGNCC 2 cut(s) 68, 426
AsuC2I CCSGG 1 cut(s) 726
AsuHPI GGTGA 3 cut(s) 31, 127, 689
AvaII GGWCC 2 cut(s) 68, 426
BalI TGGCCA 1 cut(s) 137
BanII GRGCYC 3 cut(s) 312, 388, 769
BbsI GAAGAC 1 cut(s) 888
Bbv12I GWGCWC 2 cut(s) 312, 769
BbvI GCAGC 3 cut(s) 193, 427, 659
BccI CCATC 1 cut(s) 676
BceAI ACGGC 1 cut(s) 8
BciT130I CCWGG 3 cut(s) 586, 688, 701
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 726
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 3 cut(s) 509, 605, 677
BisI GCNGC 4 cut(s) 207, 230, 416, 648
BlpI GCTNAGC 1 cut(s) 801
BlsI GCNGC 4 cut(s) 208, 231, 417, 649
BmcAI AGTACT 1 cut(s) 865
Bme1390I CCNGG 4 cut(s) 586, 688, 701, 726
Bme18I GGWCC 2 cut(s) 68, 426
BmgT120I GGNCC 2 cut(s) 68, 426
BmiI GGNNCC 2 cut(s) 590, 730
BmrFI CCNGG 4 cut(s) 586, 688, 701, 726
BmsI GCATC 1 cut(s) 45
BpiI GAAGAC 1 cut(s) 888
BpmI CTGGAG 1 cut(s) 440
Bpu1102I GCTNAGC 1 cut(s) 801
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 726
BsaBI GATNNNNATC 2 cut(s) 523, 567
BsaHI GRCGYC 1 cut(s) 362
BsaJI CCNNGG 4 cut(s) 91, 584, 687, 700
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 725
Bse1I ACTGG 1 cut(s) 687
Bse8I GATNNNNATC 2 cut(s) 523, 567
BseBI CCWGG 3 cut(s) 586, 688, 701
BseDI CCNNGG 4 cut(s) 91, 584, 687, 700
BseGI GGATG 3 cut(s) 12, 397, 876
BseJI GATNNNNATC 2 cut(s) 523, 567
BseLI CCNNNNNNNGG 2 cut(s) 97, 725
BseMII CTCAG 2 cut(s) 348, 792
BseNI ACTGG 1 cut(s) 687
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 3 cut(s) 193, 427, 659
BsgI GTGCAG 1 cut(s) 434
Bsh1285I CGRYCG 1 cut(s) 844
BshFI GGCC 2 cut(s) 137, 686
BsiEI CGRYCG 1 cut(s) 844
BsiHKAI GWGCWC 2 cut(s) 312, 769
BsiSI CCGG 2 cut(s) 33, 726
BslFI GGGAC 2 cut(s) 269, 702
BslI CCNNNNNNNGG 2 cut(s) 97, 725
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 2 cut(s) 269, 702
BsnI GGCC 2 cut(s) 137, 686
Bsp1286I GDGCHC 3 cut(s) 312, 388, 769
Bsp143I GATC 3 cut(s) 3, 250, 640
Bsp1720I GCTNAGC 1 cut(s) 801
BspACI CCGC 2 cut(s) 161, 229
BspANI GGCC 2 cut(s) 137, 686
BspCNI CTCAG 2 cut(s) 349, 793
BspLI GGNNCC 2 cut(s) 590, 730
BspQI GCTCTTC 1 cut(s) 376
BsrI ACTGG 1 cut(s) 687
BssECI CCNNGG 4 cut(s) 91, 584, 687, 700
BssMI GATC 3 cut(s) 3, 250, 640
BssNI GRCGYC 1 cut(s) 362
Bst2UI CCWGG 3 cut(s) 586, 688, 701
Bst4CI ACNGT 3 cut(s) 301, 503, 894
Bst6I CTCTTC 3 cut(s) 321, 376, 508
BstACI GRCGYC 1 cut(s) 362
BstC8I GCNNGC 1 cut(s) 220
BstDEI CTNAG 2 cut(s) 357, 801
BstENI CCTNNNNNAGG 1 cut(s) 95
BstF5I GGATG 3 cut(s) 12, 397, 876
BstKTI GATC 3 cut(s) 6, 253, 643
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 3 cut(s) 3, 250, 640
BstMCI CGRYCG 1 cut(s) 844
BstMWI GCNNNNNNNGC 2 cut(s) 215, 316
BstNI CCWGG 3 cut(s) 586, 688, 701
BstSCI CCNGG 4 cut(s) 584, 686, 699, 724
BstV1I GCAGC 3 cut(s) 193, 427, 659
BstV2I GAAGAC 1 cut(s) 888
BstXI CCANNNNNNTGG 1 cut(s) 761
BsuRI GGCC 2 cut(s) 137, 686
BtsCI GGATG 3 cut(s) 12, 397, 876
BtsIMutI CAGTG 2 cut(s) 680, 890
Cac8I GCNNGC 1 cut(s) 220
Cfr13I GGNCC 2 cut(s) 68, 426
CseI GACGC 1 cut(s) 351
Csp6I GTAC 2 cut(s) 343, 864
CspCI CAANNNNNGTGG 2 cut(s) 721, 756
CviAII CATG 3 cut(s) 20, 389, 900
CviQI GTAC 2 cut(s) 343, 864
DdeI CTNAG 2 cut(s) 357, 801
DpnI GATC 3 cut(s) 5, 252, 642
DpnII GATC 3 cut(s) 3, 250, 640
DraI TTTAAA 1 cut(s) 750
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
EaeI YGGCCR 1 cut(s) 135
Eam1104I CTCTTC 3 cut(s) 321, 376, 508
EarI CTCTTC 3 cut(s) 321, 376, 508
Ecl136II GAGCTC 2 cut(s) 310, 767
Eco24I GRGCYC 3 cut(s) 312, 388, 769
Eco32I GATATC 1 cut(s) 848
Eco47I GGWCC 2 cut(s) 68, 426
Eco53kI GAGCTC 2 cut(s) 310, 767
EcoICRI GAGCTC 2 cut(s) 310, 767
EcoNI CCTNNNNNAGG 1 cut(s) 95
EcoRII CCWGG 3 cut(s) 584, 686, 699
EcoRV GATATC 1 cut(s) 848
EcoT38I GRGCYC 3 cut(s) 312, 388, 769
FaeI CATG 3 cut(s) 23, 392, 903
FaqI GGGAC 2 cut(s) 269, 702
FatI CATG 3 cut(s) 19, 388, 899
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 4 cut(s) 207, 230, 416, 648
FokI GGATG 2 cut(s) 384, 883
FriOI GRGCYC 3 cut(s) 312, 388, 769
Fsp4HI GCNGC 4 cut(s) 207, 230, 416, 648
FspBI CTAG 3 cut(s) 509, 605, 677
GluI GCNGC 4 cut(s) 207, 230, 416, 648
GsuI CTGGAG 1 cut(s) 440
HaeIII GGCC 2 cut(s) 137, 686
HapII CCGG 2 cut(s) 33, 726
HgaI GACGC 1 cut(s) 351
Hin1I GRCGYC 1 cut(s) 362
Hin1II CATG 3 cut(s) 23, 392, 903
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HindIII AAGCTT 1 cut(s) 220
HinfI GANTC 6 cut(s) 29, 178, 242, 519, 809, 827
HpaII CCGG 2 cut(s) 33, 726
HphI GGTGA 3 cut(s) 31, 127, 689
Hpy166II GTNNAC 3 cut(s) 28, 71, 778
Hpy188I TCNGA 4 cut(s) 58, 358, 790, 880
Hpy188III TCNNGA 3 cut(s) 313, 438, 473
Hpy8I GTNNAC 3 cut(s) 28, 71, 778
HpyAV CCTTC 4 cut(s) 52, 474, 649, 829
HpyCH4III ACNGT 3 cut(s) 301, 503, 894
HpyCH4V TGCA 4 cut(s) 415, 497, 647, 663
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 316
HpyF3I CTNAG 2 cut(s) 357, 801
Hsp92I GRCGYC 1 cut(s) 362
Hsp92II CATG 3 cut(s) 23, 392, 903
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 250, 640
LguI GCTCTTC 1 cut(s) 376
LmnI GCTCC 2 cut(s) 307, 764
Lsp1109I GCAGC 3 cut(s) 193, 427, 659
LweI GCATC 1 cut(s) 45
MaeI CTAG 3 cut(s) 509, 605, 677
MaeIII GTNAC 1 cut(s) 295
MalI GATC 3 cut(s) 5, 252, 642
MboI GATC 3 cut(s) 3, 250, 640
MboII GAAGA 7 cut(s) 338, 393, 464, 525, 545, 803, 888
MhlI GDGCHC 3 cut(s) 312, 388, 769
MlsI TGGCCA 1 cut(s) 137
MluCI AATT 7 cut(s) 84, 131, 143, 321, 538, 745, 794
MluNI TGGCCA 1 cut(s) 137
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 81
Mox20I TGGCCA 1 cut(s) 137
MroXI GAANNNNTTC 1 cut(s) 552
MscI TGGCCA 1 cut(s) 137
MseI TTAA 1 cut(s) 749
MslI CAYNNNNRTG 1 cut(s) 78
Msp20I TGGCCA 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 418
MspI CCGG 2 cut(s) 33, 726
MspR9I CCNGG 4 cut(s) 586, 688, 701, 726
MvaI CCWGG 3 cut(s) 586, 688, 701
MwoI GCNNNNNNNGC 2 cut(s) 215, 316
NciI CCSGG 1 cut(s) 726
NdeI CATATG 1 cut(s) 196
NdeII GATC 3 cut(s) 3, 250, 640
NlaIII CATG 3 cut(s) 23, 392, 903
NlaIV GGNNCC 2 cut(s) 590, 730
NmeAIII GCCGAG 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 295
PciSI GCTCTTC 1 cut(s) 376
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PdmI GAANNNNTTC 1 cut(s) 552
PfeI GAWTC 5 cut(s) 178, 242, 519, 809, 827
PkrI GCNGC 4 cut(s) 208, 231, 417, 649
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
Psp124BI GAGCTC 2 cut(s) 312, 769
Psp6I CCWGG 3 cut(s) 584, 686, 699
PspGI CCWGG 3 cut(s) 584, 686, 699
PspN4I GGNNCC 2 cut(s) 590, 730
PspPI GGNCC 2 cut(s) 68, 426
PvuII CAGCTG 1 cut(s) 418
RsaI GTAC 2 cut(s) 344, 865
RsaNI GTAC 2 cut(s) 343, 864
RseI CAYNNNNRTG 1 cut(s) 78
SacI GAGCTC 2 cut(s) 312, 769
SapI GCTCTTC 1 cut(s) 376
SaqAI TTAA 1 cut(s) 749
SatI GCNGC 4 cut(s) 207, 230, 416, 648
Sau3AI GATC 3 cut(s) 3, 250, 640
Sau96I GGNCC 2 cut(s) 68, 426
ScaI AGTACT 1 cut(s) 865
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 4 cut(s) 586, 688, 701, 726
SduI GDGCHC 3 cut(s) 312, 388, 769
SfaNI GCATC 1 cut(s) 45
SinI GGWCC 2 cut(s) 68, 426
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 7 cut(s) 84, 131, 143, 321, 538, 745, 794
SsiI CCGC 2 cut(s) 161, 229
SspI AATATT 2 cut(s) 624, 760
SspMI CTAG 3 cut(s) 509, 605, 677
SstI GAGCTC 2 cut(s) 312, 769
StyD4I CCNGG 4 cut(s) 584, 686, 699, 724
TaaI ACNGT 3 cut(s) 301, 503, 894
TaqI TCGA 5 cut(s) 48, 181, 234, 546, 571
TaqII GACCGA 1 cut(s) 858
TasI AATT 7 cut(s) 84, 131, 143, 321, 538, 745, 794
TatI WGTACW 2 cut(s) 342, 863
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 5 cut(s) 178, 242, 519, 809, 827
Tru1I TTAA 1 cut(s) 749
Tru9I TTAA 1 cut(s) 749
TscAI CASTG 2 cut(s) 687, 897
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 3 cut(s) 206, 415, 647
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 6 cut(s) 96, 386, 532, 683, 887, 888
TspGWI ACGGA 1 cut(s) 839
TspRI CASTG 2 cut(s) 687, 897
VpaK11BI GGWCC 2 cut(s) 68, 426
XagI CCTNNNNNAGG 1 cut(s) 95
XapI RAATTY 4 cut(s) 84, 143, 538, 745
XcmI CCANNNNNNNNNTGG 1 cut(s) 897
XmnI GAANNNNTTC 1 cut(s) 552
XspI CTAG 3 cut(s) 509, 605, 677
ZrmI AGTACT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.