Rw4G013600

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000435
Physical Location & Seq
Reverse (-)
31762939 .. 31767024
4086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013600.1

Sequence Viewer

Length: 1032 bp
ATGGCGAGCATCAGGAGGATGAAGCTGGGGTCGCAAGGCCTGGAGGTCTCGGCTCAAGGGCTGGGTTGCGTGGGCATGTCCGTCTTCTCTGGCCCCCCGAAGCCTGAAGCCGACATGATCAAGCTCATCCACCACGCCATCGACGCCGGCGTCACCTTCCTTGATACCGCTGACGTTTACGGTCCCTTCACCAACGAACTTCTTCTTGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAATTGGCCACCAAATTTGGTATCAGCTTTGAGGACAACAAGAGAGAGATTATTCGAGGCGACCCGGCATATGTCAGGTCTGCTTGTGAAGGAAGCTTGAAACGACTTGGTGTAGATTCTATTGATCTCTATTATCAGCATCGGATTGACACTCGTGTCCCCATCGAAGTCACGGTAGGGGAGCTCAAGAAACTTGTTGAAGAGGGTAAGATAAAGTACATTGGTCTGTCTGAGGCGTCAGCTTCAACAATAAGAAGAGCCCATGCTGTTCATCCAATAACTGCTGTGCAGCTGGAGTGGTCCTTGTGGTCAAGAGATGTGGAGGAAGAAATAGTTCCTACTTGTCGGGAACTTGGCATTGGTATTGTTGCATACAGTCCTCTAGCAAGAGGATTTTTTTCACTGGGGGCCAAGTTCATTGAAAGTCTTCCGAACAATGATTCCCGAAAGAAGTTGCCCAGGTTCCAAGCTGAAAACCTAGAGCATAATGAAACAATATTCCAGCGGGTTAGTGAACTTGCAGCAAGGAAGGGATGTACCCCATCTCAACTTGCCCTGGCTTGGATTCATCACCAAGGAAATGATGTGTGCCCTATACCCGGAACTACCAAGATTGAGAATTTTGACCAGAACATTAAGGCTCTATCTGTAAAACTGACACCAGAAGAAATGGCTGAGCTTGAATCTTTTGCTTCGGCAGATGCTGTTAAGGGCGACAGATATCAGACTGGCATCAGTACTTGGTTGAACTCTGAAACTCCACCACTGTCTTCATGGAAAGCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

343

Amino Acids

37.69

Weight (kDa)

6.11

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 24 - 311 4.3e-70 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10810 AT1G10810 AT1G60680 AT1G60690 AT1G60710 AT1G60730 AT1G60730 AT1G60750
fragaria_vesca FvH4_4g12490
malus_domestica MD04G1026100.v1.1 MD09G1032900.v1.1 MD13G1279900.v1.1
prunus_persica Prupe.1G175500_v2.0.a1 Prupe.1G175600_v2.0.a1 Prupe.1G175700_v2.0.a1 Prupe.1G175800_v2.0.a1 Prupe.2G050800_v2.0.a1
pyrus_communis pycom04g02210 pycom04g02230 pycom111g02620 pycom13g29370
rosa_chinensis RchiOBHm_Chr4g0409691 RchiOBHm_Chr4g0410671 RchiOBHm_Chr4g0410821 RchiOBHm_Chr4g0411011 RchiOBHm_Chr4g0411051 RchiOBHm_Chr4g0411101 RchiOBHm_Chr4g0411151 RchiOBHm_Chr4g0411171 RchiOBHm_Chr4g0411201
rosa_laevigata RLG00000008369 RLG00000008372 RLG00000008374 RLG00000008376 RLG00000008378 RLG00000008381 RLG00000008383 RLG00000008395
rosa_multiflora Rmu_co8497103.1_g000001 Rmu_co8514481.1_g000001 Rmu_sc0000543.1_g000005 Rmu_sc0000543.1_g000006 Rmu_sc0000543.1_g000024 Rmu_sc0000944.1_g000002 Rmu_sc0000944.1_g000011 Rmu_sc0001244.1_g000006 Rmu_sc0001244.1_g000014 Rmu_sc0001244.1_g000017 Rmu_sc0001244.1_g000020 Rmu_sc0003150.1_g000001 Rmu_sc0007879.1_g000001 Rmu_sc0007879.1_g000007 Rmu_sc0009499.1_g000008 Rmu_sc0029727.1_g000001
rosa_roxburghii Rroxscaffold_5G00354230 Rroxscaffold_5G00355560 Rroxscaffold_5G00355580 Rroxscaffold_5G00355600 Rroxscaffold_5G00355650 Rroxscaffold_5G00355710 Rroxscaffold_5G00355730 Rroxscaffold_5G00355760 Rroxscaffold_5G00355800 Rroxscaffold_5G00355840 Rroxscaffold_5G00355850
rosa_rugosa Rorug04G0096400 Rorug04G0105400 Rorug04G0110600 Rorug04G0110700 Rorug05G0412600 Rorug07G0180500
rosa_samantha Rh4AG157500 Rh4BG162500 Rh4BG163400 Rh4CG175300 Rh4CG176100 Rh4CG177100 Rh4CG177200 Rh4CG177400 Rh4CG177500 Rh4CG178000 Rh4CG178100 Rh4CG178300 Rh4DG159900 Rh4DG160200
rosa_wichuraiana Rw4G013600 Rw4G013610 Rw4G013660 Rw4G013690 Rw4G013700 Rw4G013710 Rw4G013720 Rw4G013730 Rw4G013740 Rw4G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 149, 398
AciI CCGC 2 cut(s) 168, 748
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 2 cut(s) 257, 862
AcuI CTGAAG 1 cut(s) 126
AcyI GRCGYC 3 cut(s) 144, 150, 479
AfaI GTAC 3 cut(s) 461, 781, 982
AfiI CCNNNNNNNGG 2 cut(s) 804, 842
AgsI TTSAA 7 cut(s) 245, 343, 443, 489, 665, 926, 991
AjnI CCWGG 3 cut(s) 39, 701, 798
AjuI GAANNNNNNNTTGG 4 cut(s) 189, 221, 585, 617
AloI GAACNNNNNNTCC 2 cut(s) 668, 700
AluBI AGCT 9 cut(s) 25, 124, 270, 339, 427, 485, 535, 713, 922
AluI AGCT 9 cut(s) 25, 124, 270, 339, 427, 485, 535, 713, 922
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 2 cut(s) 52, 228
AlwNI CAGNNNCTG 1 cut(s) 947
AoxI GGCC 4 cut(s) 37, 91, 249, 651
ApeKI GCWGC 2 cut(s) 532, 764
ApoI RAATTY 2 cut(s) 257, 862
Asp700I GAANNNNTTC 3 cut(s) 201, 576, 669
AspS9I GGNCC 4 cut(s) 92, 182, 543, 651
AsuC2I CCSGG 2 cut(s) 308, 843
AsuHPI GGTGA 4 cut(s) 145, 181, 241, 806
AvaII GGWCC 2 cut(s) 182, 543
BaeGI GKGCMC 1 cut(s) 836
BalI TGGCCA 1 cut(s) 251
BanII GRGCYC 2 cut(s) 429, 505
BauI CACGAG 1 cut(s) 396
BbsI GAAGAC 3 cut(s) 76, 662, 1005
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 544, 776
BccI CCATC 3 cut(s) 146, 413, 793
BciT130I CCWGG 3 cut(s) 41, 703, 800
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 2 cut(s) 308, 843
BcoDI GTCTC 2 cut(s) 52, 228
BfaI CTAG 2 cut(s) 626, 722
BisI GCNGC 2 cut(s) 533, 765
BlpI GCTNAGC 1 cut(s) 918
BlsI GCNGC 2 cut(s) 534, 766
BmcAI AGTACT 1 cut(s) 982
Bme1390I CCNGG 5 cut(s) 41, 308, 703, 800, 843
Bme18I GGWCC 2 cut(s) 182, 543
BmgT120I GGNCC 4 cut(s) 92, 182, 543, 651
BmiI GGNNCC 4 cut(s) 94, 184, 652, 707
BmrFI CCNGG 5 cut(s) 41, 308, 703, 800, 843
BmrI ACTGGG 1 cut(s) 656
BmsI GCATC 4 cut(s) 18, 391, 934, 984
BmuI ACTGGG 1 cut(s) 656
BpiI GAAGAC 3 cut(s) 76, 662, 1005
BpmI CTGGAG 2 cut(s) 62, 557
Bpu1102I GCTNAGC 1 cut(s) 918
BpuEI CTTGAG 2 cut(s) 39, 413
BpuMI CCSGG 2 cut(s) 308, 843
BsaHI GRCGYC 3 cut(s) 144, 150, 479
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 3 cut(s) 701, 798, 817
Bsc4I CCNNNNNNNGG 2 cut(s) 804, 842
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 651, 976
BseBI CCWGG 3 cut(s) 41, 703, 800
BseDI CCNNGG 3 cut(s) 701, 798, 817
BseGI GGATG 4 cut(s) 24, 126, 514, 782
BseLI CCNNNNNNNGG 2 cut(s) 804, 842
BseMII CTCAG 2 cut(s) 465, 909
BseNI ACTGG 2 cut(s) 651, 976
BseSI GKGCMC 1 cut(s) 836
BseXI GCAGC 2 cut(s) 544, 776
BseYI CCCAGC 2 cut(s) 25, 61
BsgI GTGCAG 1 cut(s) 551
BshFI GGCC 4 cut(s) 39, 93, 251, 653
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 3 cut(s) 147, 308, 843
BslFI GGGAC 2 cut(s) 168, 386
BslI CCNNNNNNNGG 2 cut(s) 804, 842
BsmAI GTCTC 2 cut(s) 52, 228
BsmFI GGGAC 2 cut(s) 168, 386
BsnI GGCC 4 cut(s) 39, 93, 251, 653
Bso31I GGTCTC 1 cut(s) 52
Bsp1286I GDGCHC 3 cut(s) 429, 505, 836
Bsp143I GATC 2 cut(s) 117, 367
Bsp1720I GCTNAGC 1 cut(s) 918
BspACI CCGC 2 cut(s) 168, 748
BspANI GGCC 4 cut(s) 39, 93, 251, 653
BspCNI CTCAG 2 cut(s) 466, 910
BspLI GGNNCC 4 cut(s) 94, 184, 652, 707
BspQI GCTCTTC 1 cut(s) 493
BspTNI GGTCTC 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 2 cut(s) 651, 976
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 701, 798, 817
BssMI GATC 2 cut(s) 117, 367
BssNI GRCGYC 3 cut(s) 144, 150, 479
BssSI CACGAG 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 817
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 3 cut(s) 41, 703, 800
Bst4CI ACNGT 4 cut(s) 182, 418, 620, 1011
Bst6I CTCTTC 2 cut(s) 438, 493
BstACI GRCGYC 3 cut(s) 144, 150, 479
BstC8I GCNNGC 2 cut(s) 7, 148
BstDEI CTNAG 2 cut(s) 474, 918
BstF5I GGATG 4 cut(s) 24, 126, 514, 782
BstKTI GATC 2 cut(s) 120, 370
BstMAI GTCTC 2 cut(s) 52, 228
BstMBI GATC 2 cut(s) 117, 367
BstMWI GCNNNNNNNGC 2 cut(s) 31, 143
BstNI CCWGG 3 cut(s) 41, 703, 800
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 5 cut(s) 39, 306, 701, 798, 841
BstSLI GKGCMC 1 cut(s) 836
BstV1I GCAGC 2 cut(s) 544, 776
BstV2I GAAGAC 3 cut(s) 76, 662, 1005
BsuRI GGCC 4 cut(s) 39, 93, 251, 653
BtsCI GGATG 4 cut(s) 24, 126, 514, 782
BtsIMutI CAGTG 2 cut(s) 644, 1007
Cac8I GCNNGC 2 cut(s) 7, 148
CaiI CAGNNNCTG 1 cut(s) 947
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 4 cut(s) 92, 182, 543, 651
CseI GACGC 3 cut(s) 139, 152, 468
Csp6I GTAC 3 cut(s) 460, 780, 981
CspCI CAANNNNNGTGG 4 cut(s) 241, 276, 543, 578
CviAII CATG 4 cut(s) 76, 115, 506, 1017
CviQI GTAC 3 cut(s) 460, 780, 981
DdeI CTNAG 2 cut(s) 474, 918
DpnI GATC 2 cut(s) 119, 369
DpnII GATC 2 cut(s) 117, 367
DrdI GACNNNNNNGTC 2 cut(s) 149, 398
DseDI GACNNNNNNGTC 2 cut(s) 149, 398
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 2 cut(s) 438, 493
EarI CTCTTC 2 cut(s) 438, 493
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 817
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 2 cut(s) 429, 505
Eco31I GGTCTC 1 cut(s) 52
Eco32I GATATC 1 cut(s) 965
Eco47I GGWCC 2 cut(s) 182, 543
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 427
EcoRII CCWGG 3 cut(s) 39, 701, 798
EcoRV GATATC 1 cut(s) 965
EcoT14I CCWWGG 1 cut(s) 817
EcoT38I GRGCYC 2 cut(s) 429, 505
ErhI CCWWGG 1 cut(s) 817
FaeI CATG 4 cut(s) 79, 118, 509, 1020
FaqI GGGAC 2 cut(s) 168, 386
FatI CATG 4 cut(s) 75, 114, 505, 1016
FauI CCCGC 1 cut(s) 741
FauNDI CATATG 1 cut(s) 313
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 533, 765
FokI GGATG 4 cut(s) 31, 113, 501, 789
FriOI GRGCYC 2 cut(s) 429, 505
Fsp4HI GCNGC 2 cut(s) 533, 765
FspBI CTAG 2 cut(s) 626, 722
GluI GCNGC 2 cut(s) 533, 765
GsaI CCCAGC 2 cut(s) 29, 65
GsuI CTGGAG 2 cut(s) 62, 557
HaeIII GGCC 4 cut(s) 39, 93, 251, 653
HapII CCGG 3 cut(s) 147, 308, 843
HgaI GACGC 3 cut(s) 139, 152, 468
Hin1I GRCGYC 3 cut(s) 144, 150, 479
Hin1II CATG 4 cut(s) 79, 118, 509, 1020
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 4 cut(s) 359, 683, 808, 926
HpaII CCGG 3 cut(s) 147, 308, 843
HphI GGTGA 4 cut(s) 145, 181, 241, 806
Hpy166II GTNNAC 2 cut(s) 178, 758
Hpy188I TCNGA 5 cut(s) 387, 475, 675, 969, 997
Hpy188III TCNNGA 5 cut(s) 13, 430, 555, 590, 687
Hpy8I GTNNAC 2 cut(s) 178, 758
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 4 cut(s) 166, 196, 326, 766
HpyCH4III ACNGT 4 cut(s) 182, 418, 620, 1011
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 3 cut(s) 532, 614, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 143
HpyF3I CTNAG 2 cut(s) 474, 918
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 3 cut(s) 144, 150, 479
Hsp92II CATG 4 cut(s) 79, 118, 509, 1020
KroI GCCGGC 1 cut(s) 146
KroNI GCCGGC 1 cut(s) 148
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 2 cut(s) 117, 367
LguI GCTCTTC 1 cut(s) 493
LmnI GCTCC 1 cut(s) 424
Lsp1109I GCAGC 2 cut(s) 544, 776
LweI GCATC 4 cut(s) 18, 391, 934, 984
MaeI CTAG 2 cut(s) 626, 722
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 2 cut(s) 151, 412
MalI GATC 2 cut(s) 119, 369
MboI GATC 2 cut(s) 117, 367
MboII GAAGA 8 cut(s) 76, 194, 455, 510, 581, 662, 920, 1005
MhlI GDGCHC 3 cut(s) 429, 505, 836
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 3 cut(s) 245, 257, 862
MluNI TGGCCA 1 cut(s) 251
Mox20I TGGCCA 1 cut(s) 251
MreI CGCCGGCG 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 146
MroXI GAANNNNTTC 3 cut(s) 201, 576, 669
MscI TGGCCA 1 cut(s) 251
MseI TTAA 2 cut(s) 879, 951
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 3 cut(s) 170, 535, 748
MspI CCGG 3 cut(s) 147, 308, 843
MspR9I CCNGG 5 cut(s) 41, 308, 703, 800, 843
MvaI CCWGG 3 cut(s) 41, 703, 800
MwoI GCNNNNNNNGC 2 cut(s) 31, 143
NaeI GCCGGC 1 cut(s) 148
NciI CCSGG 2 cut(s) 308, 843
NdeI CATATG 1 cut(s) 313
NdeII GATC 2 cut(s) 117, 367
NgoMIV GCCGGC 1 cut(s) 146
NlaIII CATG 4 cut(s) 79, 118, 509, 1020
NlaIV GGNNCC 4 cut(s) 94, 184, 652, 707
NmeAIII GCCGAG 1 cut(s) 29
NmuCI GTSAC 2 cut(s) 151, 412
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 493
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PdiI GCCGGC 1 cut(s) 148
PdmI GAANNNNTTC 3 cut(s) 201, 576, 669
PfeI GAWTC 4 cut(s) 359, 683, 808, 926
PkrI GCNGC 2 cut(s) 534, 766
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 3 cut(s) 39, 701, 798
PspFI CCCAGC 2 cut(s) 25, 61
PspGI CCWGG 3 cut(s) 39, 701, 798
PspN4I GGNNCC 4 cut(s) 94, 184, 652, 707
PspPI GGNCC 4 cut(s) 92, 182, 543, 651
PstNI CAGNNNCTG 1 cut(s) 947
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 3 cut(s) 461, 781, 982
RsaNI GTAC 3 cut(s) 460, 780, 981
SacI GAGCTC 1 cut(s) 429
SapI GCTCTTC 1 cut(s) 493
SaqAI TTAA 2 cut(s) 879, 951
SatI GCNGC 2 cut(s) 533, 765
Sau3AI GATC 2 cut(s) 117, 367
Sau96I GGNCC 4 cut(s) 92, 182, 543, 651
ScaI AGTACT 1 cut(s) 982
ScrFI CCNGG 5 cut(s) 41, 308, 703, 800, 843
SduI GDGCHC 3 cut(s) 429, 505, 836
SfaNI GCATC 4 cut(s) 18, 391, 934, 984
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 2 cut(s) 182, 543
SmlI CTYRAG 2 cut(s) 54, 428
SmoI CTYRAG 2 cut(s) 54, 428
Sse9I AATT 3 cut(s) 245, 257, 862
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 2 cut(s) 168, 748
SspI AATATT 1 cut(s) 741
SspMI CTAG 2 cut(s) 626, 722
SstI GAGCTC 1 cut(s) 429
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 5 cut(s) 39, 306, 701, 798, 841
StyI CCWWGG 1 cut(s) 817
TaaI ACNGT 4 cut(s) 182, 418, 620, 1011
TaiI ACGT 1 cut(s) 177
TaqI TCGA 3 cut(s) 141, 298, 408
TasI AATT 3 cut(s) 245, 257, 862
TatI WGTACW 2 cut(s) 459, 980
TfiI GAWTC 4 cut(s) 359, 683, 808, 926
Tru1I TTAA 2 cut(s) 879, 951
Tru9I TTAA 2 cut(s) 879, 951
TscAI CASTG 2 cut(s) 651, 1014
TseFI GTSAC 2 cut(s) 151, 412
TseI GCWGC 2 cut(s) 532, 764
Tsp45I GTSAC 2 cut(s) 151, 412
TspDTI ATGAA 6 cut(s) 35, 503, 649, 747, 800, 1005
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 2 cut(s) 651, 1014
VpaK11BI GGWCC 2 cut(s) 182, 543
XapI RAATTY 2 cut(s) 257, 862
XceI RCATGY 1 cut(s) 79
XcmI CCANNNNNNNNNTGG 1 cut(s) 1014
XmnI GAANNNNTTC 3 cut(s) 201, 576, 669
XspI CTAG 2 cut(s) 626, 722
ZrmI AGTACT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.