pycom111g02620

reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0000435
Physical Location & Seq
Forward (+)
1576886 .. 1579233
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02620.4

Sequence Viewer

Length: 864 bp
ATGCCATCAACAGCGGCGTCACCTTCCTTGATACCTCTGACATCTACGGTCCCTTCACCAACGAAATCCTTCTTGGCAAGGCTCTCAAGGGAGGGGTACGAGACAAGGTTGAATTGGCCACTAAGTTTGGTCTCAGATTTGTGGAGAACAAGATGGAGGTGTGACCCTGCTTATGTGAGAGCTGCTCTCGAGAGTAGCTTGAAGCGCCTTGGTGTTGATTCTGTTGATCGATCTATTATCAGCATCGCATTGACAACTCCGTCCCCATTGAAGTCACGGTTTGGTAAGGTAAAGTACATTGGTCTATCCGAGGCCTCAGCTTCAACGATAAGAAGAGCCCATGCTGTTCATCCTATAACAGCTGTGCAGTTGGAGTGGTCCTTGTGGTCAAGAGATGTCGAACAAGAAATAATTCCTACTTGTCGGGAGCTTGGCATTGGTATTGTTGCGTATAGTCCTTTAGGAAGAGGATTCTTCGCATCAGGTGCCAAGTTTGTCGAAAACCTTGCCCACGATGATTCCCGAAAGAATCTACCGAGGTTCCAACCCGAAAATGTAGAGCACAACAATACACTATTTGAGCGAGTTAGTGATCTTGCAGCAAGGAAAGGGTGCACACCATCTCAGCTAGCATTGGCCTGGGTTCATCACCAAGGGAATGATGTGTGTCCTATCCCCGGAACCACCAAGATTGCAAATTTTGACCAGAATATCGCAGCTCTGTCTGTTAAACTGACACCGGAAGAGTTGGCTGAGCTTGAATCTTATGCTTCAGCAGATGCCGTTAAAGGTGATAGATATCCGAACTACTATAGCACTTGGTCGAACACCGAAACCCCGCCACTGTCTTCGTCAAACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

288

Amino Acids

31.7

Weight (kDa)

8.32

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10810 AT1G10810 AT1G60680 AT1G60690 AT1G60710 AT1G60730 AT1G60730 AT1G60750
fragaria_vesca FvH4_4g12490
malus_domestica MD04G1026100.v1.1 MD09G1032900.v1.1 MD13G1279900.v1.1
prunus_persica Prupe.1G175500_v2.0.a1 Prupe.1G175600_v2.0.a1 Prupe.1G175700_v2.0.a1 Prupe.1G175800_v2.0.a1 Prupe.2G050800_v2.0.a1
pyrus_communis pycom04g02210 pycom04g02230 pycom111g02620 pycom13g29370
rosa_chinensis RchiOBHm_Chr4g0409691 RchiOBHm_Chr4g0410671 RchiOBHm_Chr4g0410821 RchiOBHm_Chr4g0411011 RchiOBHm_Chr4g0411051 RchiOBHm_Chr4g0411101 RchiOBHm_Chr4g0411151 RchiOBHm_Chr4g0411171 RchiOBHm_Chr4g0411201
rosa_laevigata RLG00000008369 RLG00000008372 RLG00000008374 RLG00000008376 RLG00000008378 RLG00000008381 RLG00000008383 RLG00000008395
rosa_multiflora Rmu_co8497103.1_g000001 Rmu_co8514481.1_g000001 Rmu_sc0000543.1_g000005 Rmu_sc0000543.1_g000006 Rmu_sc0000543.1_g000024 Rmu_sc0000944.1_g000002 Rmu_sc0000944.1_g000011 Rmu_sc0001244.1_g000006 Rmu_sc0001244.1_g000014 Rmu_sc0001244.1_g000017 Rmu_sc0001244.1_g000020 Rmu_sc0003150.1_g000001 Rmu_sc0007879.1_g000001 Rmu_sc0007879.1_g000007 Rmu_sc0009499.1_g000008 Rmu_sc0029727.1_g000001
rosa_roxburghii Rroxscaffold_5G00354230 Rroxscaffold_5G00355560 Rroxscaffold_5G00355580 Rroxscaffold_5G00355600 Rroxscaffold_5G00355650 Rroxscaffold_5G00355710 Rroxscaffold_5G00355730 Rroxscaffold_5G00355760 Rroxscaffold_5G00355800 Rroxscaffold_5G00355840 Rroxscaffold_5G00355850
rosa_rugosa Rorug04G0096400 Rorug04G0105400 Rorug04G0110600 Rorug04G0110700 Rorug05G0412600 Rorug07G0180500
rosa_samantha Rh4AG157500 Rh4BG162500 Rh4BG163400 Rh4CG175300 Rh4CG176100 Rh4CG177100 Rh4CG177200 Rh4CG177400 Rh4CG177500 Rh4CG178000 Rh4CG178100 Rh4CG178300 Rh4DG159900 Rh4DG160200
rosa_wichuraiana Rw4G013600 Rw4G013610 Rw4G013660 Rw4G013690 Rw4G013700 Rw4G013710 Rw4G013720 Rw4G013730 Rw4G013740 Rw4G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 485
AciI CCGC 2 cut(s) 14, 839
AcoI YGGCCR 1 cut(s) 116
AcsI RAATTY 1 cut(s) 697
AcuI CTGAAG 1 cut(s) 756
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 2 cut(s) 98, 296
AfiI CCNNNNNNNGG 1 cut(s) 677
AgsI TTSAA 5 cut(s) 112, 202, 271, 324, 761
AjnI CCWGG 1 cut(s) 638
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 8 cut(s) 182, 198, 320, 362, 430, 628, 719, 757
AluI AGCT 8 cut(s) 182, 198, 320, 362, 430, 628, 719, 757
Alw21I GWGCWC 2 cut(s) 564, 617
Alw26I GTCTC 2 cut(s) 95, 136
Alw44I GTGCAC 1 cut(s) 613
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 3 cut(s) 116, 312, 636
ApaLI GTGCAC 1 cut(s) 613
ApeKI GCWGC 3 cut(s) 182, 599, 716
ApoI RAATTY 1 cut(s) 697
Asp700I GAANNNNTTC 2 cut(s) 68, 411
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 49, 378
AsuC2I CCSGG 1 cut(s) 678
AsuHPI GGTGA 4 cut(s) 12, 48, 641, 803
AsuNHI GCTAGC 1 cut(s) 628
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 2 cut(s) 49, 378
BaeGI GKGCMC 1 cut(s) 617
BalI TGGCCA 1 cut(s) 118
BanI GGYRCC 1 cut(s) 485
BanII GRGCYC 1 cut(s) 340
BbsI GAAGAC 1 cut(s) 840
Bbv12I GWGCWC 2 cut(s) 564, 617
BbvCI CCTCAGC 1 cut(s) 316
BbvI GCAGC 3 cut(s) 169, 611, 728
BccI CCATC 3 cut(s) 13, 147, 628
BceAI ACGGC 1 cut(s) 767
BcgI CGANNNNNNTGC 2 cut(s) 488, 522
BciT130I CCWGG 1 cut(s) 640
BcnI CCSGG 1 cut(s) 678
BcoDI GTCTC 2 cut(s) 95, 136
BfaI CTAG 1 cut(s) 629
BfmI CTRYAG 1 cut(s) 811
BfoI RGCGCY 1 cut(s) 208
BisI GCNGC 4 cut(s) 15, 183, 600, 717
BlpI GCTNAGC 1 cut(s) 753
BlsI GCNGC 4 cut(s) 16, 184, 601, 718
Bme1390I CCNGG 2 cut(s) 640, 678
Bme18I GGWCC 2 cut(s) 49, 378
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 49, 378
BmiI GGNNCC 4 cut(s) 51, 487, 542, 682
BmrFI CCNGG 2 cut(s) 640, 678
BmsI GCATC 3 cut(s) 252, 488, 769
BmtI GCTAGC 1 cut(s) 632
BpiI GAAGAC 1 cut(s) 840
BplI GAGNNNNNCTC 4 cut(s) 169, 201, 171, 203
Bpu10I CCTNAGC 1 cut(s) 316
Bpu1102I GCTNAGC 1 cut(s) 753
BpuEI CTTGAG 1 cut(s) 70
BpuMI CCSGG 1 cut(s) 678
Bsa29I ATCGAT 1 cut(s) 229
BsaBI GATNNNNATC 1 cut(s) 798
BsaHI GRCGYC 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 6 cut(s) 208, 309, 536, 639, 652, 676
BsaWI WCCGGW 1 cut(s) 739
Bsc4I CCNNNNNNNGG 1 cut(s) 677
Bse8I GATNNNNATC 1 cut(s) 798
BseBI CCWGG 1 cut(s) 640
BseCI ATCGAT 1 cut(s) 229
BseDI CCNNGG 6 cut(s) 208, 309, 536, 639, 652, 676
BseGI GGATG 1 cut(s) 349
BseJI GATNNNNATC 1 cut(s) 798
BseLI CCNNNNNNNGG 1 cut(s) 677
BseMII CTCAG 4 cut(s) 147, 330, 638, 744
BseSI GKGCMC 1 cut(s) 617
BseXI GCAGC 3 cut(s) 169, 611, 728
BsgI GTGCAG 1 cut(s) 386
BshFI GGCC 3 cut(s) 118, 314, 638
BshNI GGYRCC 1 cut(s) 485
BshVI ATCGAT 1 cut(s) 229
BsiHKAI GWGCWC 2 cut(s) 564, 617
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 2 cut(s) 678, 740
BslFI GGGAC 2 cut(s) 35, 247
BslI CCNNNNNNNGG 1 cut(s) 677
BsmAI GTCTC 2 cut(s) 95, 136
BsmFI GGGAC 2 cut(s) 35, 247
BsnI GGCC 3 cut(s) 118, 314, 638
Bso31I GGTCTC 1 cut(s) 136
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 3 cut(s) 340, 564, 617
Bsp143I GATC 3 cut(s) 226, 230, 592
Bsp1720I GCTNAGC 1 cut(s) 753
BspACI CCGC 2 cut(s) 14, 839
BspANI GGCC 3 cut(s) 118, 314, 638
BspCNI CTCAG 4 cut(s) 146, 329, 637, 745
BspDI ATCGAT 1 cut(s) 229
BspLI GGNNCC 4 cut(s) 51, 487, 542, 682
BspOI GCTAGC 1 cut(s) 632
BspQI GCTCTTC 1 cut(s) 328
BspT107I GGYRCC 1 cut(s) 485
BspTNI GGTCTC 1 cut(s) 136
BssECI CCNNGG 6 cut(s) 208, 309, 536, 639, 652, 676
BssMI GATC 3 cut(s) 226, 230, 592
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 2 cut(s) 208, 652
Bst2UI CCWGG 1 cut(s) 640
Bst4CI ACNGT 3 cut(s) 49, 279, 846
Bst6I CTCTTC 3 cut(s) 328, 460, 738
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 485
BstC8I GCNNGC 1 cut(s) 630
BstDEI CTNAG 5 cut(s) 122, 133, 316, 624, 753
BstF5I GGATG 1 cut(s) 349
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 3 cut(s) 229, 233, 595
BstMAI GTCTC 2 cut(s) 95, 136
BstMBI GATC 3 cut(s) 226, 230, 592
BstMWI GCNNNNNNNGC 2 cut(s) 204, 485
BstNI CCWGG 1 cut(s) 640
BstSCI CCNGG 2 cut(s) 638, 676
BstSFI CTRYAG 1 cut(s) 811
BstSLI GKGCMC 1 cut(s) 617
BstV1I GCAGC 3 cut(s) 169, 611, 728
BstV2I GAAGAC 1 cut(s) 840
Bsu15I ATCGAT 1 cut(s) 229
BsuRI GGCC 3 cut(s) 118, 314, 638
BsuTUI ATCGAT 1 cut(s) 229
BtgZI GCGATG 1 cut(s) 229
BtsCI GGATG 1 cut(s) 349
BtsIMutI CAGTG 1 cut(s) 842
Cac8I GCNNGC 1 cut(s) 630
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 2 cut(s) 49, 378
ClaI ATCGAT 1 cut(s) 229
CseI GACGC 1 cut(s) 6
Csp6I GTAC 2 cut(s) 97, 295
CspCI CAANNNNNGTGG 4 cut(s) 108, 143, 673, 708
CviAII CATG 1 cut(s) 341
CviQI GTAC 2 cut(s) 97, 295
DdeI CTNAG 5 cut(s) 122, 133, 316, 624, 753
DpnI GATC 3 cut(s) 228, 232, 594
DpnII GATC 3 cut(s) 226, 230, 592
DrdI GACNNNNNNGTC 1 cut(s) 259
DseDI GACNNNNNNGTC 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 116
Eam1104I CTCTTC 3 cut(s) 328, 460, 738
EarI CTCTTC 3 cut(s) 328, 460, 738
Eco130I CCWWGG 2 cut(s) 208, 652
Eco147I AGGCCT 1 cut(s) 314
Eco24I GRGCYC 1 cut(s) 340
Eco31I GGTCTC 1 cut(s) 136
Eco32I GATATC 1 cut(s) 800
Eco47I GGWCC 2 cut(s) 49, 378
Eco57I CTGAAG 1 cut(s) 756
Eco88I CYCGRG 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 638
EcoRV GATATC 1 cut(s) 800
EcoT14I CCWWGG 2 cut(s) 208, 652
EcoT38I GRGCYC 1 cut(s) 340
ErhI CCWWGG 2 cut(s) 208, 652
FaeI CATG 1 cut(s) 344
FaiI YATR 6 cut(s) 174, 342, 356, 453, 768, 813
FaqI GGGAC 2 cut(s) 35, 247
FatI CATG 1 cut(s) 340
FauI CCCGC 1 cut(s) 846
Fnu4HI GCNGC 4 cut(s) 15, 183, 600, 717
FokI GGATG 1 cut(s) 336
FriOI GRGCYC 1 cut(s) 340
Fsp4HI GCNGC 4 cut(s) 15, 183, 600, 717
FspBI CTAG 1 cut(s) 629
GlaI GCGC 1 cut(s) 206
GluI GCNGC 4 cut(s) 15, 183, 600, 717
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 3 cut(s) 118, 314, 638
HapII CCGG 2 cut(s) 678, 740
HgaI GACGC 1 cut(s) 6
HhaI GCGC 1 cut(s) 207
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HinfI GANTC 5 cut(s) 218, 471, 518, 529, 761
HpaII CCGG 2 cut(s) 678, 740
HphI GGTGA 4 cut(s) 12, 48, 641, 803
Hpy166II GTNNAC 1 cut(s) 615
Hpy188I TCNGA 4 cut(s) 39, 136, 310, 804
Hpy188III TCNNGA 5 cut(s) 188, 190, 390, 425, 522
Hpy8I GTNNAC 1 cut(s) 615
HpyAV CCTTC 3 cut(s) 33, 63, 79
HpyCH4III ACNGT 3 cut(s) 49, 279, 846
HpyCH4V TGCA 4 cut(s) 367, 599, 615, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 204, 485
HpyF3I CTNAG 5 cut(s) 122, 133, 316, 624, 753
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 1 cut(s) 205
Kzo9I GATC 3 cut(s) 226, 230, 592
LguI GCTCTTC 1 cut(s) 328
LmnI GCTCC 1 cut(s) 427
LpnPI CCDG 7 cut(s) 180, 468, 625, 652, 691, 719, 753
Lsp1109I GCAGC 3 cut(s) 169, 611, 728
LweI GCATC 3 cut(s) 252, 488, 769
MaeI CTAG 1 cut(s) 629
MaeIII GTNAC 3 cut(s) 18, 161, 273
MalI GATC 3 cut(s) 228, 232, 594
MboI GATC 3 cut(s) 226, 230, 592
MboII GAAGA 5 cut(s) 345, 466, 477, 755, 840
MhlI GDGCHC 3 cut(s) 340, 564, 617
MlsI TGGCCA 1 cut(s) 118
MluCI AATT 3 cut(s) 112, 411, 697
MluNI TGGCCA 1 cut(s) 118
MmeI TCCRAC 2 cut(s) 351, 568
MnlI CCTC 7 cut(s) 45, 85, 150, 304, 325, 461, 531
Mox20I TGGCCA 1 cut(s) 118
MroXI GAANNNNTTC 2 cut(s) 68, 411
MscI TGGCCA 1 cut(s) 118
MseI TTAA 3 cut(s) 729, 786, 862
Msp20I TGGCCA 1 cut(s) 118
MspA1I CMGCKG 2 cut(s) 14, 362
MspI CCGG 2 cut(s) 678, 740
MspR9I CCNGG 2 cut(s) 640, 678
MvaI CCWGG 1 cut(s) 640
MwoI GCNNNNNNNGC 2 cut(s) 204, 485
NciI CCSGG 1 cut(s) 678
NdeII GATC 3 cut(s) 226, 230, 592
NheI GCTAGC 1 cut(s) 628
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 4 cut(s) 51, 487, 542, 682
NmuCI GTSAC 3 cut(s) 18, 161, 273
PaeR7I CTCGAG 1 cut(s) 188
PceI AGGCCT 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 328
PdmI GAANNNNTTC 2 cut(s) 68, 411
PfeI GAWTC 5 cut(s) 218, 471, 518, 529, 761
PkrI GCNGC 4 cut(s) 16, 184, 601, 718
Psp6I CCWGG 1 cut(s) 638
PspGI CCWGG 1 cut(s) 638
PspN4I GGNNCC 4 cut(s) 51, 487, 542, 682
PspPI GGNCC 2 cut(s) 49, 378
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 2 cut(s) 98, 296
RsaNI GTAC 2 cut(s) 97, 295
SapI GCTCTTC 1 cut(s) 328
SaqAI TTAA 3 cut(s) 729, 786, 862
SatI GCNGC 4 cut(s) 15, 183, 600, 717
Sau3AI GATC 3 cut(s) 226, 230, 592
Sau96I GGNCC 2 cut(s) 49, 378
ScrFI CCNGG 2 cut(s) 640, 678
SduI GDGCHC 3 cut(s) 340, 564, 617
SfaNI GCATC 3 cut(s) 252, 488, 769
SfcI CTRYAG 1 cut(s) 811
Sfr274I CTCGAG 1 cut(s) 188
SinI GGWCC 2 cut(s) 49, 378
SlaI CTCGAG 1 cut(s) 188
SmlI CTYRAG 2 cut(s) 85, 188
SmoI CTYRAG 2 cut(s) 85, 188
Sse9I AATT 3 cut(s) 112, 411, 697
SseBI AGGCCT 1 cut(s) 314
SsiI CCGC 2 cut(s) 14, 839
SspMI CTAG 1 cut(s) 629
StuI AGGCCT 1 cut(s) 314
StyD4I CCNGG 2 cut(s) 638, 676
StyI CCWWGG 2 cut(s) 208, 652
TaaI ACNGT 3 cut(s) 49, 279, 846
TaqI TCGA 5 cut(s) 189, 229, 399, 498, 824
TasI AATT 3 cut(s) 112, 411, 697
TatI WGTACW 1 cut(s) 294
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 5 cut(s) 218, 471, 518, 529, 761
Tru1I TTAA 3 cut(s) 729, 786, 862
Tru9I TTAA 3 cut(s) 729, 786, 862
TscAI CASTG 1 cut(s) 849
TseFI GTSAC 3 cut(s) 18, 161, 273
TseI GCWGC 3 cut(s) 182, 599, 716
Tsp45I GTSAC 3 cut(s) 18, 161, 273
TspDTI ATGAA 2 cut(s) 338, 635
TspGWI ACGGA 1 cut(s) 249
TspRI CASTG 1 cut(s) 849
VneI GTGCAC 1 cut(s) 613
VpaK11BI GGWCC 2 cut(s) 49, 378
XapI RAATTY 1 cut(s) 697
XhoI CTCGAG 1 cut(s) 188
XmnI GAANNNNTTC 2 cut(s) 68, 411
XspI CTAG 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.